The Experts below are selected from a list of 6 Experts worldwide ranked by ideXlab platform
Gil Benard - One of the best experts on this subject based on the ideXlab platform.
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the role of interleukin 10 in the differential expression of interleukin 12p70 and its β2 receptor on patients with active or treated paracoccidioidomycosis and healthy infected subjects
Clinical Immunology, 2005Co-Authors: Carla C Romano, Alberto Jose Da Silva Duarte, Maria Jose Soares Mendesgiannini, Gil BenardAbstract:Abstract Paracoccidioidomycosis patients present an Antigen-specific Th1 immunosuppression. To better understand this phenomenon, we evaluated the interleukin (IL)-12 pathway by measuring IL-12p70 production and CD3 + T cell expression of the IL-12 receptor (IL-12R)β1/β2 chains, induced with the main Fungus Antigen (gp43) and a control Antigen, from Candida albicans (CMA). We showed that gp43-induced IL-12p70 production and IL-12Rβ2 expression were significantly decreased in acute and chronic patients as compared to healthy subjects cured from PCM or healthy infected subjects from endemic areas. Interestingly, the healthy infected subjects had higher gp43-induced IL-12p70 production and β2 expression than the cured subjects. The addition of a neutralizing anti-IL-10 antibody to the cultures increased IL-12p70 levels and β2 expression in acute and chronic patients to levels observed in cured subjects. Conversely, addition of the cytokine IL-10 strongly inhibited both parameters in the latter group. In conclusion, we have shown that paracoccidioidomycosis-related Th1 immunosuppression is associated with down-modulation of the IL-12 pathway, that IL-10 may participate in this process, and that patients cured from paracoccidioidomycosis may not fully recover their immune responsiveness.
Carla C Romano - One of the best experts on this subject based on the ideXlab platform.
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the role of interleukin 10 in the differential expression of interleukin 12p70 and its β2 receptor on patients with active or treated paracoccidioidomycosis and healthy infected subjects
Clinical Immunology, 2005Co-Authors: Carla C Romano, Alberto Jose Da Silva Duarte, Maria Jose Soares Mendesgiannini, Gil BenardAbstract:Abstract Paracoccidioidomycosis patients present an Antigen-specific Th1 immunosuppression. To better understand this phenomenon, we evaluated the interleukin (IL)-12 pathway by measuring IL-12p70 production and CD3 + T cell expression of the IL-12 receptor (IL-12R)β1/β2 chains, induced with the main Fungus Antigen (gp43) and a control Antigen, from Candida albicans (CMA). We showed that gp43-induced IL-12p70 production and IL-12Rβ2 expression were significantly decreased in acute and chronic patients as compared to healthy subjects cured from PCM or healthy infected subjects from endemic areas. Interestingly, the healthy infected subjects had higher gp43-induced IL-12p70 production and β2 expression than the cured subjects. The addition of a neutralizing anti-IL-10 antibody to the cultures increased IL-12p70 levels and β2 expression in acute and chronic patients to levels observed in cured subjects. Conversely, addition of the cytokine IL-10 strongly inhibited both parameters in the latter group. In conclusion, we have shown that paracoccidioidomycosis-related Th1 immunosuppression is associated with down-modulation of the IL-12 pathway, that IL-10 may participate in this process, and that patients cured from paracoccidioidomycosis may not fully recover their immune responsiveness.
Alberto Jose Da Silva Duarte - One of the best experts on this subject based on the ideXlab platform.
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the role of interleukin 10 in the differential expression of interleukin 12p70 and its β2 receptor on patients with active or treated paracoccidioidomycosis and healthy infected subjects
Clinical Immunology, 2005Co-Authors: Carla C Romano, Alberto Jose Da Silva Duarte, Maria Jose Soares Mendesgiannini, Gil BenardAbstract:Abstract Paracoccidioidomycosis patients present an Antigen-specific Th1 immunosuppression. To better understand this phenomenon, we evaluated the interleukin (IL)-12 pathway by measuring IL-12p70 production and CD3 + T cell expression of the IL-12 receptor (IL-12R)β1/β2 chains, induced with the main Fungus Antigen (gp43) and a control Antigen, from Candida albicans (CMA). We showed that gp43-induced IL-12p70 production and IL-12Rβ2 expression were significantly decreased in acute and chronic patients as compared to healthy subjects cured from PCM or healthy infected subjects from endemic areas. Interestingly, the healthy infected subjects had higher gp43-induced IL-12p70 production and β2 expression than the cured subjects. The addition of a neutralizing anti-IL-10 antibody to the cultures increased IL-12p70 levels and β2 expression in acute and chronic patients to levels observed in cured subjects. Conversely, addition of the cytokine IL-10 strongly inhibited both parameters in the latter group. In conclusion, we have shown that paracoccidioidomycosis-related Th1 immunosuppression is associated with down-modulation of the IL-12 pathway, that IL-10 may participate in this process, and that patients cured from paracoccidioidomycosis may not fully recover their immune responsiveness.
Maria Jose Soares Mendesgiannini - One of the best experts on this subject based on the ideXlab platform.
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the role of interleukin 10 in the differential expression of interleukin 12p70 and its β2 receptor on patients with active or treated paracoccidioidomycosis and healthy infected subjects
Clinical Immunology, 2005Co-Authors: Carla C Romano, Alberto Jose Da Silva Duarte, Maria Jose Soares Mendesgiannini, Gil BenardAbstract:Abstract Paracoccidioidomycosis patients present an Antigen-specific Th1 immunosuppression. To better understand this phenomenon, we evaluated the interleukin (IL)-12 pathway by measuring IL-12p70 production and CD3 + T cell expression of the IL-12 receptor (IL-12R)β1/β2 chains, induced with the main Fungus Antigen (gp43) and a control Antigen, from Candida albicans (CMA). We showed that gp43-induced IL-12p70 production and IL-12Rβ2 expression were significantly decreased in acute and chronic patients as compared to healthy subjects cured from PCM or healthy infected subjects from endemic areas. Interestingly, the healthy infected subjects had higher gp43-induced IL-12p70 production and β2 expression than the cured subjects. The addition of a neutralizing anti-IL-10 antibody to the cultures increased IL-12p70 levels and β2 expression in acute and chronic patients to levels observed in cured subjects. Conversely, addition of the cytokine IL-10 strongly inhibited both parameters in the latter group. In conclusion, we have shown that paracoccidioidomycosis-related Th1 immunosuppression is associated with down-modulation of the IL-12 pathway, that IL-10 may participate in this process, and that patients cured from paracoccidioidomycosis may not fully recover their immune responsiveness.
David Gottlieb - One of the best experts on this subject based on the ideXlab platform.
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the identification and use of a three Fungus Antigen combination to generate t cell products with activity against pathogenic filamentous fungi and yeasts for clinical cell therapy
Blood, 2014Co-Authors: Shiva Deo, Balaji Virassamy, Catriona Halliday, Leighton Clancy, Wieland Meyer, Sharon C A Chen, Tania C Sorrell, David GottliebAbstract:Abstract Invasive fungal diseases (IFDs) caused by the filamentous fungal pathogens of the genera Aspergillus, fusarium, zygomycetes, scedosporium and the yeast candida are the most important causes of Fungus related deaths in immunosuppressed hematology patients1. Clinical efficacy of A. fumigatus specific T cells has been demonstrated in haploidentical hematopoietic stem cell transplant (HSCT) recipients2. We recently published a method to culture A. fumigatus specific T cells from normal allogeneic donors using procedures compliant with the code of Good Manufacturing Practice3. The safety and efficacy of cells generated using our method is currently being tested in a Phase 1 study in HSCT recipients. Here, we investigated the possibility of manufacturing a single T cell product with activity against a broad target range of fungal pathogens for clinical adoptive immunotherapy. We made water-soluble lysates from germinated spores of fungal pathogens, A. flavus, A. terreus, C. albicans, C. krusei, F. oxysporum, F. solani, R. oryzae and S. prolificans and used these to pulse monocyte derived dendritic cells (MoDC) from PBMC of 4 normal donors. Pulsed MoDC were washed, irradiated and used to stimulate autologous PBMC on Days 0 and 7. Cells were expanded with the addition of IL-2, IL-7 and IL-15 from Days 7 to 21. Cell numbers, phenotype and Fungus-specificity were assessed on Day 21. Cross-reactivity of cultured T cells was tested against lysates of other fungi to identify a combination of Antigens likely to induce broad anti-fungal T cell reactivity. Products were generated from PBMC using either no selection or TNFα capture on Day 7 of culture to select Antigen-specific cells. Three products were generated from G-CSF mobilized peripheral blood stem cell products (PBSC) without selection. Antifungal activity was mapped to specific HLA molecules using blocking antibodies to HLA-DR, -DP and –DQ. In cultures from normal donor PBMC, expansion occurred with lysates from all fungi (range 2.3-109.6 fold) generating 85-97% T cells of which >80% were CD4+ T cells. The percentage of Fungus-specific (TNFα+) CD4+ cells were A flavus 6.8±5.5%, A terreus 13.2±12.8%, C albicans 10.5±8.5%, C krusei 6.4±6.8%, F oxysporum 6.4±3.8%, F solani 5.2±7.8%, R oryzae 7.6±6.4 and S prolificans 4.4±3.8% (n=4). T cells also produced IFNγ and IL-2. T cells from cultures generated with Aspergillus, Fusarium and Scedosporium cross-reacted with one another and with lysates from most other fungi. We selected a combination of A. terreus, C. krusei and R. oryzae to generate multiFungus T cell products from PBMC and PBSC. All but one multiFungus culture generated >89% T cells. The majority of T cells had terminally differentiated effector and effector memory phenotypes. Regulatory T cells were Blocking HLA-DR, but not -DP or -DQ on Antigen presenting cells abrogated Fungus-specific cytokine production by cultured T cells (n=7). Antifungal activity was maintained when MoDC from partially HLA-DRB1 matched allogeneic donors were used to present fungal Antigens to cultured T cells (n=7). In contrast, no antifungal activity was observed when MoDC from completely HLA-DRB1 mismatched donors were used to stimulate fungal T cells (n=4). We demonstrate that similarly to A. fumigatus specific T cells, T cells specific for other clinically relevant fungi can be expanded in vitro. We have developed a method to manufacture a T cell product with activity against multiple clinically relevant fungi, using blood or stem cells of healthy donors as starting material. The use of TNFα capture did not increase the number or purity of Fungus-specific cells in cultures. The clinical utility of infusions of multiFungus responsive T cell products in prophylaxis and treatment of invasive fungal disease needs to be tested clinically. References 1Neofytos D, Horn D et al, Clinical Infectious Diseases 2009 2Perruccio K, Tosti A et al, Blood 2005 3Gaundar S, Clancy L et al, Cytotherapy 2012 Disclosures No relevant conflicts of interest to declare.
