The Experts below are selected from a list of 27588 Experts worldwide ranked by ideXlab platform
Rodríguez Gerzaín - One of the best experts on this subject based on the ideXlab platform.
-
Hombre de 18 años con síndrome verrugoso tropical: ¿leishmaniasis o esporotricosis?
'Instituto Nacional de Salud (Colombia)', 2021Co-Authors: Macías Paola, Ordóñez Juliana, Arenas Claudia M., Rodríguez GerzaínAbstract:Tropical verrucous syndrome (TVS) covers infectious, chronic, granulomatous skin conditions. They appear with plaques, nodules or ulcers with a warty surface which give the name to the syndrome. It includes forms of chromoblastomycosis, sporotrichosis, paracoccidioidomycosis, lobomycosis, leishmaniasis and tuberculosis verrucosa cutis pathologies with ample distribution in tropical and subtropical areas. The diagnosis may be difficult with confusión among them, which is more frequent between sporotrichosis and leishmaniasis. In order to differentiate them one must accede clinical, epidemiologic, intradermal rections, direct smears, skin biopsies, Cultures, immunofluorescence and PCR techniques, several of which are not commonly used. We present a 18 year old man with extensive verrucous placques in one knee, interpreted by clinic, epidemiology and by biopsy as verrucous cutaneous leishmaniasis. He was treated with Glucantime R for twenty days without improvement. A new biopsy was made that was also diagnosed as cutaneous leishmaniasis. Revision of both biopsies showed inflammation with abscedated granulomas with presence of asteroid spototrichotic bodies at the center of the granulomas, that led to the diagnosis of sporotrichosis, which was confirmed by Fungus Culture. The patient cured with Itraconazol® treatment. Clinical and epidemiological findings of leishmaniasis and sporotrichosis can be similar. For this reason, skin biopsy and other paraclinic studies are necesary to stablish the proper diagnosis. The asteroid sporotrichotic body is patognomonic of this mycosis. We review essentials concepts of leishmaniasis and sporotrichosis and criteria for differentiate them.El síndrome verrugoso tropical (SVT) comprende entidades cutáneas infecciosas, crónicas y granulomatosas. Cursan con placas, nódulos o úlceras verrugosas, que dan el nombre al síndrome. Incluye: cromoblastomicosis, esporotricosis, paracoccidioidomicosis, lobomicosis, leishmaniasis y tuberculosis verrucosa cutis, patologías con amplia distribución en áreas tropicales y subtropicales. Pueden ser de diagnóstico difícil y confundirse entre sí, lo cual es más frecuente entre esporotricosis y leishmaniasis. Para distinguirlas se recurre a criterios clínicos, epidemiológicos, intradermorreacciones, exámenes directos, biopsias, cultivos, inmunofluorescencia y PCR, varios de los cuales no son de uso común. El diagnóstico preciso conduce al tratamiento adecuado. Presentamos un hombre de 18 años con extensas placas verrugosas de una rodilla, interpretadas por clínica, epidemiología y biopsia como leishmaniasis verrucosa. Se trató con Glucantime® durante 20 días. No presentó mejoría por lo cual se tomó nueva biopsia que también se diagnosticó como LC. La revisión de ambas demostró inflamación con granulomas abscedados, con presencia de cuerpos asteroides esporotricósicos, que condujeron al diagnóstico de esporotricosis, que se confirmó con cultivo del hongo. El paciente curó con tratamiento con Itraconazol. La clínica y la epidemiología de la leishmaniasis y la esporotricosis pueden ser semejantes por lo cual la biopsia y los estudios paraclínicos son esenciales para establecer el diagnóstico. El cuerpo asteroide esporotricósico es patognomónico de esta entidad. Revisamos conceptos esenciales de estas entidades y los criterios para diferenciarlas
-
Hombre de 18 años con síndrome verrugoso tropical: ¿leishmaniasis o esporotricosis?
'Instituto Nacional de Salud (Colombia)', 2021Co-Authors: Macías Paola, Ordóñez Juliana, Arenas Claudia M., Rodríguez GerzaínAbstract:The tropical verrucous syndrome includes infectious, chronic, and granulomatous skin conditions appearing with plaques, nodules, or ulcers with a warty surface which gives name to the syndrome. It includes forms of chromoblastomycosis, sporotrichosis, paracoccidioidomycosis, lobomycosis, leishmaniasis, and tuberculosis verrucosa cutis with ample distribution in tropical and subtropical areas. The diagnoses may be difficult and confused among them, especially between sporotrichosis and leishmaniasis. Clinical, epidemiologic, intradermal reactions, direct smears, skin biopsies, Cultures, immunofluorescence, and PCR are used to differentiate them, although several of these methods are not commonly used.We present an 18-year-old man with extensive verrucous plaques in one knee interpreted by clinic, epidemiology, and biopsy as verrucous cutaneous leishmaniasis. He was treated with Glucantime® for 20 days without improvement. A new biopsy was made that was also interpreted as cutaneous leishmaniasis. The revision of both biopsies showed inflammation with abscessed granulomas and asteroid sporotrichotic bodies at the center of the granulomas that led to the diagnosis of sporotrichosis later confirmed by the Fungus Culture. The patient responded to the treatment with itraconazole. As clinical and epidemiological findings of leishmaniasis and sporotrichosis can be similar, skin biopsy and other paraclinical studies are necessary to establish a proper diagnosis. The asteroid sporotrichotic body is pathognomonic of this mycosis. We review here the essential concepts of leishmaniasis and sporotrichosis and the criteria to differentiate them.El síndrome verrugoso tropical comprende condiciones cutáneas infecciosas, crónicas y granulomatosas que cursan con placas, nódulos o úlceras verrugosas, de ahí su nombre. Este síndrome incluye la cromoblastomicosis, la esporotricosis, la paracoccidioidomicosis, la lobomicosis, la leishmaniasis y la tuberculosis cutánea verrugosa, todas ellas enfermedades de amplia distribución en áreas tropicales y subtropicales. Sus diagnósticos pueden ser difíciles y confundirse entre sí, lo cual es más frecuente entre la esporotricosis y la leishmaniasis. Para distinguirlas se recurre a criterios clínicos y epidemiológicos, y a métodos diagnósticos como intradermorreacción, examen directo, biopsia, cultivo, inmunofluorescencia y PCR, algunos de los cuales no son de uso común. El diagnóstico preciso conduce al tratamiento adecuado. Se presenta el caso de un hombre de 18 años con extensas placas verrugosas en una rodilla, inicialmente interpretadas como leishmaniasis verrugosa por la clínica, la epidemiología y la biopsia. Se le trató con Glucantime® durante 20 días, pero no presentó mejoría, por lo que se tomó una nueva biopsia que también se interpretó como leishmaniasis cutánea. La revisión de ambas biopsias evidenció inflamación con granulomas abscedados y presencia de cuerpos asteroides esporotricósicos, que condujeron al diagnóstico de esporotricosis, el cual se confirmó luego con el cultivo del hongo. Las lesiones remitieron con la administración de itraconazol.La clínica y la epidemiología de la leishmaniasis y las de la esporotricosis pueden ser semejantes, por lo que la biopsia y los estudios de laboratorio son esenciales para establecer el diagnóstico. El cuerpo asteroide esporotricósico es patognomónico de esta entidad
Brian Reavy - One of the best experts on this subject based on the ideXlab platform.
-
association of sequences in the coat protein readthrough domain of potato mop top virus with transmission by spongospora subterranea
Journal of General Virology, 1998Co-Authors: Brian Reavy, G H Cowan, Lesley TorranceAbstract:A monofungal Culture of Spongospora subterranea was unable to acquire and transmit the T isolate of potato mop-top pomovirus (PMTV-T), which has been maintained by manual transmission in the laboratory for 30 years. A recently obtained field isolate (PMTV-S) was efficiently acquired and transmitted by the same Fungus Culture. Sequence analysis of the readthrough (RT) protein-coding region of PMTV-S showed the presence of an additional 543 nt in the 3' half of the coding region relative to that of PMTV-T. These additional nucleotides preserved the reading frame of the RT protein and inserted 181 amino acids into the RT protein. This was confirmed by a comparison by immunoblotting of the sizes of the RT protein of PMTV-T and other recent isolates of PMTV.
-
acquisition and transmission of potato mop to furovirus by a Culture of spongospora subterranea f sp subterranea derived from a single cystosorus
Annals of Applied Biology, 1995Co-Authors: Lesley Torrance, Brian ReavyAbstract:SUMMARY Potato mop-top virus (PMTV) was detected by ELISA in primary zoospores from four out of six isolates of Spongospora subterranea f.sp. subterranea. One virus-free isolate (N) of S. subterranea was used to acquire PMTV from potato roots and to transmit the virus to healthy plants. A mono-fungal Culture of S. subterranea (isolate N) was derived by infecting tomato plant roots with a single cystosorus. The Culture was used successfully to acquire PMTV from the roots of infected Nicotiana debneyi plants that had been manually inoculated with virus isolates, and subsequently to transmit the virus to healthy bait plants. These experiments confirm that S. subterranea is a vector of PMTV. Two PMTV isolates that had been maintained by manual inoculation for 19 and 21 passages were also acquired and transmitted by the Fungus Culture.
Lesley Torrance - One of the best experts on this subject based on the ideXlab platform.
-
association of sequences in the coat protein readthrough domain of potato mop top virus with transmission by spongospora subterranea
Journal of General Virology, 1998Co-Authors: Brian Reavy, G H Cowan, Lesley TorranceAbstract:A monofungal Culture of Spongospora subterranea was unable to acquire and transmit the T isolate of potato mop-top pomovirus (PMTV-T), which has been maintained by manual transmission in the laboratory for 30 years. A recently obtained field isolate (PMTV-S) was efficiently acquired and transmitted by the same Fungus Culture. Sequence analysis of the readthrough (RT) protein-coding region of PMTV-S showed the presence of an additional 543 nt in the 3' half of the coding region relative to that of PMTV-T. These additional nucleotides preserved the reading frame of the RT protein and inserted 181 amino acids into the RT protein. This was confirmed by a comparison by immunoblotting of the sizes of the RT protein of PMTV-T and other recent isolates of PMTV.
-
acquisition and transmission of potato mop to furovirus by a Culture of spongospora subterranea f sp subterranea derived from a single cystosorus
Annals of Applied Biology, 1995Co-Authors: Lesley Torrance, Brian ReavyAbstract:SUMMARY Potato mop-top virus (PMTV) was detected by ELISA in primary zoospores from four out of six isolates of Spongospora subterranea f.sp. subterranea. One virus-free isolate (N) of S. subterranea was used to acquire PMTV from potato roots and to transmit the virus to healthy plants. A mono-fungal Culture of S. subterranea (isolate N) was derived by infecting tomato plant roots with a single cystosorus. The Culture was used successfully to acquire PMTV from the roots of infected Nicotiana debneyi plants that had been manually inoculated with virus isolates, and subsequently to transmit the virus to healthy bait plants. These experiments confirm that S. subterranea is a vector of PMTV. Two PMTV isolates that had been maintained by manual inoculation for 19 and 21 passages were also acquired and transmitted by the Fungus Culture.
Paul E Verweij - One of the best experts on this subject based on the ideXlab platform.
-
in vitro activity of chlorhexidine compared with seven antifungal agents against 98 fusarium isolates recovered from fungal keratitis patients
Antimicrobial Agents and Chemotherapy, 2019Co-Authors: Claudy Oliveira Dos Santos, Eva Kolwijck, Marlou Tehupeiorykooreman, Abdullah M S Alhatmi, Einoti Matayan, Matthew J Burton, Cathrien A Eggink, Paul E VerweijAbstract:Fungal keratitis is a common but severe eye infection in tropical and subtropical areas of the world. In regions with a temperate climate, the frequency of infection is rising in patients with contact lenses and following trauma. Early and adequate therapy is important to prevent disease progression and loss of vision. The management of Fusarium keratitis is complex, and the optimal treatment is not well defined. We investigated the in vitro activity of chlorhexidine and seven antifungal agents against a well-characterized collection of Fusarium isolates recovered from patients with Fusarium keratitis. The Fungus Culture collection of the Center of Expertise in Mycology Radboudumc/CWZ was searched for Fusariurn isolates that were Cultured from cornea scrapings, ocular biopsy specimens, eye swabs, and contact lens fluid containers from patients with suspected keratitis. The Fusariurn isolates that were Cultured from patients with confirmed keratitis were all identified using conventional and molecular techniques. Antifungal susceptibility testing was performed according to the EUCAST broth microdilution reference method. The antifungal agents tested included amphotericin B, voriconazole, posaconazole, miconazole, natamycin, 5-fluorocytosine, and caspofungin. In addition, the activity of chlorhexidine was determined. The fungal Culture collection contained 98 Fusarium isolates of confirmed fungal keratitis cases from 83 Dutch patients and 15 Tanzanian patients. The isolates were collected between 2007 and 2017. Fusarium oxysporurn (n = 24, 24.5%) was the most frequently isolated species followed by Fusarium solani sense stricto (n = 18, 18.4%) and Fusarium petroliphilum (n = 11, 11.2%). Amphotericin B showed the most favorable in vitro inhibition of Fusariurn species followed by natamycin, voriconazole, and chlorhexidine, while 5-fluorocytosine, posaconazole, miconazole, and caspofungin showed no relevant inhibiting effect. However, chlorhexidine showed fungicidal activity against 90% of F. oxysporum strains and 100% of the F. solani strains. Our study supports the clinical efficacy of chlorhexidine and therefore warrants its further clinical evaluation for primary therapy of fungal keratitis, particularly in low and middle income countries where fungal keratitis is much more frequent and, currently, antifungal eye drops are often unavailable.
Macías Paola - One of the best experts on this subject based on the ideXlab platform.
-
Hombre de 18 años con síndrome verrugoso tropical: ¿leishmaniasis o esporotricosis?
'Instituto Nacional de Salud (Colombia)', 2021Co-Authors: Macías Paola, Ordóñez Juliana, Arenas Claudia M., Rodríguez GerzaínAbstract:Tropical verrucous syndrome (TVS) covers infectious, chronic, granulomatous skin conditions. They appear with plaques, nodules or ulcers with a warty surface which give the name to the syndrome. It includes forms of chromoblastomycosis, sporotrichosis, paracoccidioidomycosis, lobomycosis, leishmaniasis and tuberculosis verrucosa cutis pathologies with ample distribution in tropical and subtropical areas. The diagnosis may be difficult with confusión among them, which is more frequent between sporotrichosis and leishmaniasis. In order to differentiate them one must accede clinical, epidemiologic, intradermal rections, direct smears, skin biopsies, Cultures, immunofluorescence and PCR techniques, several of which are not commonly used. We present a 18 year old man with extensive verrucous placques in one knee, interpreted by clinic, epidemiology and by biopsy as verrucous cutaneous leishmaniasis. He was treated with Glucantime R for twenty days without improvement. A new biopsy was made that was also diagnosed as cutaneous leishmaniasis. Revision of both biopsies showed inflammation with abscedated granulomas with presence of asteroid spototrichotic bodies at the center of the granulomas, that led to the diagnosis of sporotrichosis, which was confirmed by Fungus Culture. The patient cured with Itraconazol® treatment. Clinical and epidemiological findings of leishmaniasis and sporotrichosis can be similar. For this reason, skin biopsy and other paraclinic studies are necesary to stablish the proper diagnosis. The asteroid sporotrichotic body is patognomonic of this mycosis. We review essentials concepts of leishmaniasis and sporotrichosis and criteria for differentiate them.El síndrome verrugoso tropical (SVT) comprende entidades cutáneas infecciosas, crónicas y granulomatosas. Cursan con placas, nódulos o úlceras verrugosas, que dan el nombre al síndrome. Incluye: cromoblastomicosis, esporotricosis, paracoccidioidomicosis, lobomicosis, leishmaniasis y tuberculosis verrucosa cutis, patologías con amplia distribución en áreas tropicales y subtropicales. Pueden ser de diagnóstico difícil y confundirse entre sí, lo cual es más frecuente entre esporotricosis y leishmaniasis. Para distinguirlas se recurre a criterios clínicos, epidemiológicos, intradermorreacciones, exámenes directos, biopsias, cultivos, inmunofluorescencia y PCR, varios de los cuales no son de uso común. El diagnóstico preciso conduce al tratamiento adecuado. Presentamos un hombre de 18 años con extensas placas verrugosas de una rodilla, interpretadas por clínica, epidemiología y biopsia como leishmaniasis verrucosa. Se trató con Glucantime® durante 20 días. No presentó mejoría por lo cual se tomó nueva biopsia que también se diagnosticó como LC. La revisión de ambas demostró inflamación con granulomas abscedados, con presencia de cuerpos asteroides esporotricósicos, que condujeron al diagnóstico de esporotricosis, que se confirmó con cultivo del hongo. El paciente curó con tratamiento con Itraconazol. La clínica y la epidemiología de la leishmaniasis y la esporotricosis pueden ser semejantes por lo cual la biopsia y los estudios paraclínicos son esenciales para establecer el diagnóstico. El cuerpo asteroide esporotricósico es patognomónico de esta entidad. Revisamos conceptos esenciales de estas entidades y los criterios para diferenciarlas
-
Hombre de 18 años con síndrome verrugoso tropical: ¿leishmaniasis o esporotricosis?
'Instituto Nacional de Salud (Colombia)', 2021Co-Authors: Macías Paola, Ordóñez Juliana, Arenas Claudia M., Rodríguez GerzaínAbstract:The tropical verrucous syndrome includes infectious, chronic, and granulomatous skin conditions appearing with plaques, nodules, or ulcers with a warty surface which gives name to the syndrome. It includes forms of chromoblastomycosis, sporotrichosis, paracoccidioidomycosis, lobomycosis, leishmaniasis, and tuberculosis verrucosa cutis with ample distribution in tropical and subtropical areas. The diagnoses may be difficult and confused among them, especially between sporotrichosis and leishmaniasis. Clinical, epidemiologic, intradermal reactions, direct smears, skin biopsies, Cultures, immunofluorescence, and PCR are used to differentiate them, although several of these methods are not commonly used.We present an 18-year-old man with extensive verrucous plaques in one knee interpreted by clinic, epidemiology, and biopsy as verrucous cutaneous leishmaniasis. He was treated with Glucantime® for 20 days without improvement. A new biopsy was made that was also interpreted as cutaneous leishmaniasis. The revision of both biopsies showed inflammation with abscessed granulomas and asteroid sporotrichotic bodies at the center of the granulomas that led to the diagnosis of sporotrichosis later confirmed by the Fungus Culture. The patient responded to the treatment with itraconazole. As clinical and epidemiological findings of leishmaniasis and sporotrichosis can be similar, skin biopsy and other paraclinical studies are necessary to establish a proper diagnosis. The asteroid sporotrichotic body is pathognomonic of this mycosis. We review here the essential concepts of leishmaniasis and sporotrichosis and the criteria to differentiate them.El síndrome verrugoso tropical comprende condiciones cutáneas infecciosas, crónicas y granulomatosas que cursan con placas, nódulos o úlceras verrugosas, de ahí su nombre. Este síndrome incluye la cromoblastomicosis, la esporotricosis, la paracoccidioidomicosis, la lobomicosis, la leishmaniasis y la tuberculosis cutánea verrugosa, todas ellas enfermedades de amplia distribución en áreas tropicales y subtropicales. Sus diagnósticos pueden ser difíciles y confundirse entre sí, lo cual es más frecuente entre la esporotricosis y la leishmaniasis. Para distinguirlas se recurre a criterios clínicos y epidemiológicos, y a métodos diagnósticos como intradermorreacción, examen directo, biopsia, cultivo, inmunofluorescencia y PCR, algunos de los cuales no son de uso común. El diagnóstico preciso conduce al tratamiento adecuado. Se presenta el caso de un hombre de 18 años con extensas placas verrugosas en una rodilla, inicialmente interpretadas como leishmaniasis verrugosa por la clínica, la epidemiología y la biopsia. Se le trató con Glucantime® durante 20 días, pero no presentó mejoría, por lo que se tomó una nueva biopsia que también se interpretó como leishmaniasis cutánea. La revisión de ambas biopsias evidenció inflamación con granulomas abscedados y presencia de cuerpos asteroides esporotricósicos, que condujeron al diagnóstico de esporotricosis, el cual se confirmó luego con el cultivo del hongo. Las lesiones remitieron con la administración de itraconazol.La clínica y la epidemiología de la leishmaniasis y las de la esporotricosis pueden ser semejantes, por lo que la biopsia y los estudios de laboratorio son esenciales para establecer el diagnóstico. El cuerpo asteroide esporotricósico es patognomónico de esta entidad
