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Sephra N Rampersad - One of the best experts on this subject based on the ideXlab platform.
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signatures of tri5 tri8 and tri11 protein sequences of Fusarium incarnatum equiseti species complex fiesc indicate differential trichothecene analogue production
Toxins, 2020Co-Authors: Ria T Villafana, Sephra N RampersadAbstract:The variability and phylogeny among TRI5, TRI8 and TRI11 nucleotide and translated protein sequences of isolates from Trinidad belonging to Fusarium incarnatum-equiseti species complex (FIESC) were compared with FIESC reference sequences. Taxa appeared to be more divergent when DNA sequences were analyzed compared to protein sequences. Neutral and non-neutral mutations in TRI protein sequences that may correspond to variability in the function and structure of the selected TRI proteins were identified. TRI5p had the lowest amino acid diversity with zero predicted non-neutral mutations. TRI5p had potentially three protein disorder regions compared to TRI8p with five protein disorder regions. The deduced TRI11p was more conserved than TRI8p of the same strains. Amino acid substitutions that may be non-neutral to protein function were only detected in diacetoxyscirpenol (DAS) and fusarenon-X (FUS-X) producers of the reference sequence subset for TRI8p and TRI11p. The deduced TRI5 and TRI8 amino acid sequences were mapped to known 3D-structure models and indicated that variations in specific protein order/disorder regions exist in these sequences which affect the overall structural conservation of TRI proteins. Assigning single or combination non-neutral mutations to a particular toxicogenic phenotype may be more representative of potential compared to using genotypic data alone, especially in the absence of wet-lab, experimental validation.
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Three-Locus Sequence Identification and Differential Tebuconazole Sensitivity Suggest Novel Fusarium equiseti Haplotype from Trinidad.
Pathogenetics, 2020Co-Authors: Ria T Villafana, Sephra N RampersadAbstract:The Fusarium incarnatum-equiseti species complex (FIESC) consists of 33 phylogenetic species according to multi-locus sequence typing (MLST) and Genealogical Concordance Phylogenetic Species Recognition (GCPSR). A multi-locus dataset consisting of nucleotide sequences of the translation elongation factor (EF-1α), calmodulin (CAM), partial RNA polymerase largest subunit (RPB1), and partial RNA polymerase second largest subunit (RPB2), was generated to distinguish among phylogenetic species within the FIESC isolates infecting bell pepper in Trinidad. Three phylogenetic species belonged to the incarnatum clade (FIESC-15, FIESC-16, and FIESC-26), and one species belonged to the Equiseti clade (FIESC-14). Specific MLST types were sensitive to 10 µg/mL of tebuconazole fungicide as a discriminatory dose. The EC50 values were significantly different among the four MLST groups, which were separated into two homogeneous groups: FIESC-26a and FIESC-14a, demonstrating the “sensitive” azole phenotype and FIESC-15a and FIESC-16a as the “less sensitive” azole phenotype. CYP51C sequences of the Trinidad isolates, although under positive selection, were without any signatures of recombination, were highly conserved, and were not correlated with these azole phenotypes. CYP51C sequences were unable to resolve the FIESC isolates as phylogenetic inference indicated polytomic branching for these sequences. This data is important to different research communities, including those studying Fusarium phytopathology, mycotoxins, and public health impacts.
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fungicide sensitivity among isolates of colletotrichum truncatum and Fusarium incarnatum equiseti species complex infecting bell pepper in trinidad
Plant Pathology Journal, 2017Co-Authors: H Ramdial, Kathryn De Abreu, Sephra N RampersadAbstract:: Bell pepper is an economically important crop worldwide; however, production is restricted by a number of fungal diseases that cause significant yield loss. Chemical control is the most common approach adopted by growers to manage a number of these diseases. Monitoring for the development to resistance to fungicides in pathogenic fungal populations is central to devising integrated pest management strategies. Two fungal species, Fusarium incarnatum-equiseti species complex (FIESC) and Colletotrichum truncatum are important pathogens of bell pepper in Trinidad. This study was carried out to determine the sensitivity of 71 isolates belonging to these two fungal species to fungicides with different modes of action based on in vitro bioassays. There was no significant difference in log effective concentration required to achieve 50% colony growth inhibition (LogEC50) values when field location and fungicide were considered for each species separately based on ANOVA analyses. However, the LogEC50 value for the Aranguez-Antracol location-fungicide combination was almost twice the value for the Maloney/Macoya-Antracol location-fungicide combination regardless of fungal species. LogEC50 values for Benomyl fungicide was also higher for C. truncatum isolates than for FIESC isolates and for any other fungicide. Cropping practices in these locations may explain the fungicide sensitivity data obtained.
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phylogeny and haplotype analysis of fungi within the Fusarium incarnatum equiseti species complex
Phytopathology, 2017Co-Authors: H Ramdial, R K Latchoo, F N Hosein, Sephra N RampersadAbstract:Fusarium spp. are ranked among the top 10 most economically and scientifically important plant-pathogenic fungi in the world and are associated with plant diseases that include fruit decay of a number of crops. Fusarium isolates infecting bell pepper in Trinidad were identified based on sequence comparisons of the translation elongation factor gene (EF-1a) with sequences of Fusarium incarnatum-equiseti species complex (FIESC) verified in the Fusarium-ID database. Eighty-two isolates were identified as belonging to one of four phylogenetic species within the subclades FIESC-1, FIESC-15, FIESC-16, and FIESC-26, with the majority of isolates belonging to FIESC-15. A comparison of the level of DNA polymorphism and phylogenetic inference for sequences of the internal transcribed spacer region (ITS1-5.8S-ITS2) and EF-1a sequences for Trinidad and Fusarium-ID type species was carried out. The ITS sequences were less informative, had lower haplotype diversity and restricted haplotype distribution, and resulted in...
Arindam Kuila - One of the best experts on this subject based on the ideXlab platform.
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Lipase production from mutagenic strain of Fusarium incarnatum KU377454 and its immobilization using Au@Ag core shells nanoparticles for application in waste cooking oil degradation
3 Biotech, 2019Co-Authors: Ritika Joshi, Rekha Sharma, Rupam Bhunia, Anand Prakash, Arindam KuilaAbstract:In the present study, lipase production from mutated strain of Fusarium incarnatum KU377454 was optimized through central composite design (CCD) based response surface methodology (RSM). The maximum lipase production (4.01 IU/mL) was obtained within 4 days of incubation using 0.1% CaCl_2 concentration and 8% wheat bran concentration. Further, salting out technique was applied for partial purification of lipase. The partially purified lipase was immobilized using Au@Ag bimetallic nanoshell. The characterization of immobilized lipase was carried out by transmission electron microscopy (TEM), field emission scanning electron microscopy (FE-SEM), Fourier transformed infrared (FTIR), energy dispersive X-ray (EDX), X-ray diffraction (XRD) and thermo gravimetric analysis (TGA). The immobilized lipase could retain its 95% of activity after 15 days of storage at 4 °C. Subsequently, Au@Ag immobilized lipase was used for the degradation of waste cooking oil (WCO), which showed higher WCO degradation (85%) compared to the free lipase mediated waste cooking oil degradation (71%). The immobilized lipase could be reused for five times without any loss of its activity.
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lipase production from Fusarium incarnatum ku377454 and its immobilization using fe3o4 nps for application in waste cooking oil degradation
Bioresource Technology Reports, 2019Co-Authors: Ritika Joshi, Rekha Sharma, Arindam KuilaAbstract:Abstract The main aim of this work was production and optimization of lipase from Fusarium incarnatum KU377454 by central composite design (CCD) based response surface methodology (RSM). The maximum lipase activity (37.80 ± 0.55 IU/mL) was obtained within 5 d of incubation. The produced lipase was partially purified by salting out technique. Then purified lipase was immobilized using Fe3O4 NPs and further nanoparticles immobilized lipase was evaluated by different characterization study (FTIR, XRD, FESEM, EDX, Zeta potential, UV-spectroscopy, and BET). Then, Fe3O4 immobilized lipase was applied for waste cooking oil (WCO) degradation. It showed 90% biodiesel product yield with major fatty acid yields of linoleic acid, oleic acid, palmitic acid, and linolenic acid. The reusability study of immobilized lipase showed that it could retain 75% of its activity after 5 cycle of waste cooking oil degradation.
Neiva Tinti Oliveira - One of the best experts on this subject based on the ideXlab platform.
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morphology phylogeny and sexual stage of Fusarium caatingaense and Fusarium pernambucanum new species of the Fusarium incarnatum equiseti species complex associated with insects in brazil
Mycologia, 2019Co-Authors: Ana Carla Silva Santos, Antonio Félix Costa, Patricia Vieira Tiago, Jose Vinicius Correia Trindade, Cristiano Souza Lima, Renan Do Nascimento Barbosa, Neiva Tinti OliveiraAbstract:ABSTRACTBased on morphological and molecular phylogenetic markers and the fertility of sexual crosses, two novel species of Fusarium associated with Dactylopius opuntiae (Hemiptera: Dactylopiidae) ...
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Dactylopius opuntiae: control by the Fusarium incarnatum–equiseti species complex and confirmation of mortality by DNA fingerprinting
Journal of Pest Science, 2017Co-Authors: Mariele Porto Carneiro-leão, Antonio Félix Costa, Patricia Vieira Tiago, Lílian Vieira Medeiros, Neiva Tinti OliveiraAbstract:Cultivation of the prickly pear cactus, Opuntia ficus - indica , an important cattle food in the semiarid region of Brazil, has been increasingly compromised by predation by the cochineal scale Dactylopius opuntiae . Entomopathogenic fungi represent a low environmental impact alternative to controlling this insect pest, and the genus Fusarium has been described as a promising agent for that task. As such, we selected isolates of the Fusarium incarnatum – equiseti species complex (FIESC) to evaluate their potential biological control of D. opuntiae and confirmed their efficiency/presence in the field through morphological and molecular characteristics. The pathogenicities of 25 isolates of FIESC obtained from D. opuntiae were evaluated. The isolates URM6782, URM6778, and URM6811 demonstrated kill rates above 45%, and good sporulation characteristics, and were thus selected for field testing. The isolate URM6782 showed the highest kill rate in the field, and amplification profiles obtained using the ISSR UBC834 marker confirmed that the isolates released in the field were in fact the causal agents of the high mortality of D. opuntiae . We also observed natural biological control by native Fusarium species present in the field. Our results suggest that the isolates URM6782 and URM6778 show significant promise for controlling D. opuntiae and that the ISSR primer UBC834 can be used to monitor those isolates when released into the field. This work represents an initial study directed toward the biological control of D. opuntiae using novel isolates from the FIESC.
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dactylopius opuntiae control by the Fusarium incarnatum equiseti species complex and confirmation of mortality by dna fingerprinting
Journal of Pest Science, 2017Co-Authors: Mariele Porto Carneiroleao, Antonio Félix Costa, Patricia Vieira Tiago, Lílian Vieira Medeiros, Neiva Tinti OliveiraAbstract:Cultivation of the prickly pear cactus, Opuntia ficus-indica, an important cattle food in the semiarid region of Brazil, has been increasingly compromised by predation by the cochineal scale Dactylopius opuntiae. Entomopathogenic fungi represent a low environmental impact alternative to controlling this insect pest, and the genus Fusarium has been described as a promising agent for that task. As such, we selected isolates of the Fusarium incarnatum–equiseti species complex (FIESC) to evaluate their potential biological control of D. opuntiae and confirmed their efficiency/presence in the field through morphological and molecular characteristics. The pathogenicities of 25 isolates of FIESC obtained from D. opuntiae were evaluated. The isolates URM6782, URM6778, and URM6811 demonstrated kill rates above 45%, and good sporulation characteristics, and were thus selected for field testing. The isolate URM6782 showed the highest kill rate in the field, and amplification profiles obtained using the ISSR UBC834 marker confirmed that the isolates released in the field were in fact the causal agents of the high mortality of D. opuntiae. We also observed natural biological control by native Fusarium species present in the field. Our results suggest that the isolates URM6782 and URM6778 show significant promise for controlling D. opuntiae and that the ISSR primer UBC834 can be used to monitor those isolates when released into the field. This work represents an initial study directed toward the biological control of D. opuntiae using novel isolates from the FIESC.
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Dactylopius opuntiae : control by the Fusarium incarnatum – equiseti species complex and confirmation of mortality by DNA fingerprinting
Journal of Pest Science, 2017Co-Authors: Mariele Porto Carneiro-leão, Antonio Félix Costa, Patricia Vieira Tiago, Lílian Vieira Medeiros, Neiva Tinti OliveiraAbstract:Cultivation of the prickly pear cactus, Opuntia ficus-indica, an important cattle food in the semiarid region of Brazil, has been increasingly compromised by predation by the cochineal scale Dactylopius opuntiae. Entomopathogenic fungi represent a low environmental impact alternative to controlling this insect pest, and the genus Fusarium has been described as a promising agent for that task. As such, we selected isolates of the Fusarium incarnatum–equiseti species complex (FIESC) to evaluate their potential biological control of D. opuntiae and confirmed their efficiency/presence in the field through morphological and molecular characteristics. The pathogenicities of 25 isolates of FIESC obtained from D. opuntiae were evaluated. The isolates URM6782, URM6778, and URM6811 demonstrated kill rates above 45%, and good sporulation characteristics, and were thus selected for field testing. The isolate URM6782 showed the highest kill rate in the field, and amplification profiles obtained using the ISSR UBC834 marker confirmed that the isolates released in the field were in fact the causal agents of the high mortality of D. opuntiae. We also observed natural biological control by native Fusarium species present in the field. Our results suggest that the isolates URM6782 and URM6778 show significant promise for controlling D. opuntiae and that the ISSR primer UBC834 can be used to monitor those isolates when released into the field. This work represents an initial study directed toward the biological control of D. opuntiae using novel isolates from the FIESC.
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Polymorphisms in entomopathogenic fusaria based on inter simple sequence repeats
Biocontrol Science and Technology, 2016Co-Authors: Patricia Vieira Tiago, Antonio Félix Costa, Ana Carla Silva Santos, Lílian Vieira Medeiros, Mariele Porto Carneiro Leão, Neiva Tinti OliveiraAbstract:ABSTRACTIsolates of Fusarium obtained from Dactylopius opuntiae (Cockerell) (Hemiptera: Dactylopiidae) demonstrated potential as biological control agents against that same insect, which is a pest on Opuntia ficus-indica L Miller. The isolates belong to two species complexes: Fusarium incarnatum-equiseti (FIESC – five species) and Fusarium fujikuroi (FFSC – one species). Twenty-eight isolates of these fungi were characterised using seven Inter Simple Sequence Repeats (ISSR) primers. The UBC841 primer differentiated all the FIESC isolates studied and the single isolate of Fusarium pseudocircinatum O’Donnell & Nirenberg at a level greater than 90% similarity for the fragment sizes. The results indicated high genetic variability among those isolates, an important characteristic for biological control, increasing the chances of finding efficient fungi for insect control. The ISSR markers UBC834 and UBC841 were found to be efficient for characterising and differentiating (DNA fingerprinting) those fungi, and c...
Ritika Joshi - One of the best experts on this subject based on the ideXlab platform.
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Lipase production from mutagenic strain of Fusarium incarnatum KU377454 and its immobilization using Au@Ag core shells nanoparticles for application in waste cooking oil degradation
3 Biotech, 2019Co-Authors: Ritika Joshi, Rekha Sharma, Rupam Bhunia, Anand Prakash, Arindam KuilaAbstract:In the present study, lipase production from mutated strain of Fusarium incarnatum KU377454 was optimized through central composite design (CCD) based response surface methodology (RSM). The maximum lipase production (4.01 IU/mL) was obtained within 4 days of incubation using 0.1% CaCl_2 concentration and 8% wheat bran concentration. Further, salting out technique was applied for partial purification of lipase. The partially purified lipase was immobilized using Au@Ag bimetallic nanoshell. The characterization of immobilized lipase was carried out by transmission electron microscopy (TEM), field emission scanning electron microscopy (FE-SEM), Fourier transformed infrared (FTIR), energy dispersive X-ray (EDX), X-ray diffraction (XRD) and thermo gravimetric analysis (TGA). The immobilized lipase could retain its 95% of activity after 15 days of storage at 4 °C. Subsequently, Au@Ag immobilized lipase was used for the degradation of waste cooking oil (WCO), which showed higher WCO degradation (85%) compared to the free lipase mediated waste cooking oil degradation (71%). The immobilized lipase could be reused for five times without any loss of its activity.
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lipase production from Fusarium incarnatum ku377454 and its immobilization using fe3o4 nps for application in waste cooking oil degradation
Bioresource Technology Reports, 2019Co-Authors: Ritika Joshi, Rekha Sharma, Arindam KuilaAbstract:Abstract The main aim of this work was production and optimization of lipase from Fusarium incarnatum KU377454 by central composite design (CCD) based response surface methodology (RSM). The maximum lipase activity (37.80 ± 0.55 IU/mL) was obtained within 5 d of incubation. The produced lipase was partially purified by salting out technique. Then purified lipase was immobilized using Fe3O4 NPs and further nanoparticles immobilized lipase was evaluated by different characterization study (FTIR, XRD, FESEM, EDX, Zeta potential, UV-spectroscopy, and BET). Then, Fe3O4 immobilized lipase was applied for waste cooking oil (WCO) degradation. It showed 90% biodiesel product yield with major fatty acid yields of linoleic acid, oleic acid, palmitic acid, and linolenic acid. The reusability study of immobilized lipase showed that it could retain 75% of its activity after 5 cycle of waste cooking oil degradation.
Rekha Sharma - One of the best experts on this subject based on the ideXlab platform.
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Lipase production from mutagenic strain of Fusarium incarnatum KU377454 and its immobilization using Au@Ag core shells nanoparticles for application in waste cooking oil degradation
3 Biotech, 2019Co-Authors: Ritika Joshi, Rekha Sharma, Rupam Bhunia, Anand Prakash, Arindam KuilaAbstract:In the present study, lipase production from mutated strain of Fusarium incarnatum KU377454 was optimized through central composite design (CCD) based response surface methodology (RSM). The maximum lipase production (4.01 IU/mL) was obtained within 4 days of incubation using 0.1% CaCl_2 concentration and 8% wheat bran concentration. Further, salting out technique was applied for partial purification of lipase. The partially purified lipase was immobilized using Au@Ag bimetallic nanoshell. The characterization of immobilized lipase was carried out by transmission electron microscopy (TEM), field emission scanning electron microscopy (FE-SEM), Fourier transformed infrared (FTIR), energy dispersive X-ray (EDX), X-ray diffraction (XRD) and thermo gravimetric analysis (TGA). The immobilized lipase could retain its 95% of activity after 15 days of storage at 4 °C. Subsequently, Au@Ag immobilized lipase was used for the degradation of waste cooking oil (WCO), which showed higher WCO degradation (85%) compared to the free lipase mediated waste cooking oil degradation (71%). The immobilized lipase could be reused for five times without any loss of its activity.
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lipase production from Fusarium incarnatum ku377454 and its immobilization using fe3o4 nps for application in waste cooking oil degradation
Bioresource Technology Reports, 2019Co-Authors: Ritika Joshi, Rekha Sharma, Arindam KuilaAbstract:Abstract The main aim of this work was production and optimization of lipase from Fusarium incarnatum KU377454 by central composite design (CCD) based response surface methodology (RSM). The maximum lipase activity (37.80 ± 0.55 IU/mL) was obtained within 5 d of incubation. The produced lipase was partially purified by salting out technique. Then purified lipase was immobilized using Fe3O4 NPs and further nanoparticles immobilized lipase was evaluated by different characterization study (FTIR, XRD, FESEM, EDX, Zeta potential, UV-spectroscopy, and BET). Then, Fe3O4 immobilized lipase was applied for waste cooking oil (WCO) degradation. It showed 90% biodiesel product yield with major fatty acid yields of linoleic acid, oleic acid, palmitic acid, and linolenic acid. The reusability study of immobilized lipase showed that it could retain 75% of its activity after 5 cycle of waste cooking oil degradation.
