The Experts below are selected from a list of 1455 Experts worldwide ranked by ideXlab platform
La´szlo´ Hornok - One of the best experts on this subject based on the ideXlab platform.
-
Genetic Interrelationships and Genome Organization of Double-Stranded RNA Elements of Fusarium poae
Virus Genes, 1999Co-Authors: Peter Compel, Istva´n Papp, Ma´rton Bibo´, Csaba Fekete, La´szlo´ HornokAbstract:The similar-sized double-stranded RNA (dsRNA) elements present in vegetatively compatible strains of Fusarium poae were always genetically related, while vegetatively incompatible strains of the fungus contained either homologous or non-homologous dsRNAs of the same size. Electron microscopic observations revealed the co-existence of encapsidated and naked dsRNA elements in the same host. A mycovirus, named FUPO-1 was purified from strain A-11 and was found to contain two kinds of dsRNA segments, dsRNA 1 and dsRNA 2. The dsRNA genome of these segments was converted to cDNA clones by reverse transcription and the clones were subjected to sequence analysis. The single long open reading frame deduced from the sequence of dsRNA 1 showed similarities to the putative coat protein genes known from other mycoviruses, while conserved motifs of an RNA-dependent RNA polymerase were identified in the predicted amino acid sequence of dsRNA 2. The genome organization and certain sequence motifs of FUPO-1 show similarities to that of the Atkinsonella hypoxylon 2H virus and the FusoV mycovirus, members of the Partitiviridae family.
-
high frequency occurrence of virus like particles with double stranded rna genome in Fusarium poae
Fems Microbiology Letters, 1995Co-Authors: Csaba Fekete, Gabor Giczey, Istvan Papp, Laszlo Szabo, La´szlo´ HornokAbstract:Fifty-five geographically different strains of Fusarium poae were assayed for the presence of extrachromosomal nucleic acid elements. All strains were found to harbour double-stranded RNA (dsRNA) elements and encapsidated virus-like particles (VLP). There were great individual differences in dsRNA patterns of the various strains, but numbers and sizes characteristic for a given isolate remained unchanged after repeated subculturing of the fungi. Morphological alterations or signs of degeneration were not observed in dsRNA-containing isolates. This is the first report on the ubiquitous occurrence of dsRNAs in a hyphomycete fungus species.
-
Research letterHigh-frequency occurrence of virus-like particles with double-stranded RNA genome in Fusarium poae
FEMS Microbiology Letters, 1995Co-Authors: Csaba Fekete, Gabor Giczey, Istvan Papp, Laszlo Szabo, La´szlo´ HornokAbstract:Fifty-five geographically different strains of Fusarium poae were assayed for the presence of extrachromosomal nucleic acid elements. All strains were found to harbour double-stranded RNA (dsRNA) elements and encapsidated virus-like particles (VLP). There were great individual differences in dsRNA patterns of the various strains, but numbers and sizes characteristic for a given isolate remained unchanged after repeated subculturing of the fungi. Morphological alterations or signs of degeneration were not observed in dsRNA-containing isolates. This is the first report on the ubiquitous occurrence of dsRNAs in a hyphomycete fungus species.
Csaba Fekete - One of the best experts on this subject based on the ideXlab platform.
-
Genetic Interrelationships and Genome Organization of Double-Stranded RNA Elements of Fusarium poae
Virus Genes, 1999Co-Authors: Peter Compel, Istva´n Papp, Ma´rton Bibo´, Csaba Fekete, La´szlo´ HornokAbstract:The similar-sized double-stranded RNA (dsRNA) elements present in vegetatively compatible strains of Fusarium poae were always genetically related, while vegetatively incompatible strains of the fungus contained either homologous or non-homologous dsRNAs of the same size. Electron microscopic observations revealed the co-existence of encapsidated and naked dsRNA elements in the same host. A mycovirus, named FUPO-1 was purified from strain A-11 and was found to contain two kinds of dsRNA segments, dsRNA 1 and dsRNA 2. The dsRNA genome of these segments was converted to cDNA clones by reverse transcription and the clones were subjected to sequence analysis. The single long open reading frame deduced from the sequence of dsRNA 1 showed similarities to the putative coat protein genes known from other mycoviruses, while conserved motifs of an RNA-dependent RNA polymerase were identified in the predicted amino acid sequence of dsRNA 2. The genome organization and certain sequence motifs of FUPO-1 show similarities to that of the Atkinsonella hypoxylon 2H virus and the FusoV mycovirus, members of the Partitiviridae family.
-
high frequency occurrence of virus like particles with double stranded rna genome in Fusarium poae
Fems Microbiology Letters, 1995Co-Authors: Csaba Fekete, Gabor Giczey, Istvan Papp, Laszlo Szabo, La´szlo´ HornokAbstract:Fifty-five geographically different strains of Fusarium poae were assayed for the presence of extrachromosomal nucleic acid elements. All strains were found to harbour double-stranded RNA (dsRNA) elements and encapsidated virus-like particles (VLP). There were great individual differences in dsRNA patterns of the various strains, but numbers and sizes characteristic for a given isolate remained unchanged after repeated subculturing of the fungi. Morphological alterations or signs of degeneration were not observed in dsRNA-containing isolates. This is the first report on the ubiquitous occurrence of dsRNAs in a hyphomycete fungus species.
-
Research letterHigh-frequency occurrence of virus-like particles with double-stranded RNA genome in Fusarium poae
FEMS Microbiology Letters, 1995Co-Authors: Csaba Fekete, Gabor Giczey, Istvan Papp, Laszlo Szabo, La´szlo´ HornokAbstract:Fifty-five geographically different strains of Fusarium poae were assayed for the presence of extrachromosomal nucleic acid elements. All strains were found to harbour double-stranded RNA (dsRNA) elements and encapsidated virus-like particles (VLP). There were great individual differences in dsRNA patterns of the various strains, but numbers and sizes characteristic for a given isolate remained unchanged after repeated subculturing of the fungi. Morphological alterations or signs of degeneration were not observed in dsRNA-containing isolates. This is the first report on the ubiquitous occurrence of dsRNAs in a hyphomycete fungus species.
Antonio Logrieco - One of the best experts on this subject based on the ideXlab platform.
-
Volatile Metabolite Profiling of Durum Wheat Kernels Contaminated by Fusarium poae
Metabolites, 2014Co-Authors: Barbara Laddomada, D.s. Presicce, Pietro Siciliano, Laura Del Coco, Miriana Durante, Francesco P. Fanizzi, Antonio LogriecoAbstract:Volatile metabolites from mold contamination have been proposed for the early identification of toxigenic fungi to prevent toxicological risks, but there are no such data available for Fusarium poae. F. poae is one of the species complexes involved in Fusarium head blight, a cereal disease that results in significant yield losses and quality reductions. The identification of volatile organic compounds associated with F. poae metabolism could provide good markers to indicate early fungal contamination. To this aim, we evaluated the volatile profile of healthy and F. poae-infected durum wheat kernels by SPME-GC/MS analysis. The production of volatile metabolites was monitored for seven days, and the time course analysis of key volatiles was determined. A total of 29 volatile markers were selected among the detected compounds, and multivariate analysis was applied to establish the relationship between potential volatile markers and fungal contamination. A range of volatile compounds, including alcohols, ketones, esters, furans and aromatics, were identified, both in contaminated and in healthy kernels. However, the overall volatile profile of infected samples and controls differed, indicating that the whole volatile profile, rather than individual volatile compounds, could be used to identify F. poae contamination of durum wheat grains.
-
trichothecene and beauvericin mycotoxin production and genetic variability in Fusarium poae isolated from wheat kernels from northern italy
Food Additives and Contaminants Part A-chemistry Analysis Control Exposure & Risk Assessment, 2010Co-Authors: Stefania Somma, Alberto Ritieni, Antonio Logrieco, C Alvarez, V Ricci, L Ferracane, Antonio MorettiAbstract:The importance and widespread incidence of Fusarium poae as a natural contaminant of wheat in different climatic areas warrants investigation into the genetic diversity and toxin profile of a northern Italy population. Eighty-one strains of F. poae isolated from durum wheat kernels, identified by species-specific polymerase chain reaction and translation elongation factor-1alpha gene sequence analysis, were genetically characterized by the amplified fragment length polymorphism (AFLP) technique and analysed by high-pressure liquid chromatography for their ability to produce the beauvericin (BEA) and trichothecene mycotoxins. A high level of variability was observed by using AFLP analyses, with the lowest level of genetic similarity among the strains being approximately 61%. Most of the strains, 95%, produced BEA at <2655 microg g(-1); 88% produced the trichothecene nivalenol at <865 microg g(-1) and 76% produced the trichothecene fusarenon-X at <167 microg g(-1). These data show that F. poae can produce high amounts of BEA together with trichothecenes, and can represent a high potential mycotoxin risk in Italy for wheat colonized by this species.
-
Trichothecene and beauvericin mycotoxin production and genetic variability in Fusarium poae isolated from wheat kernels from northern Italy.
Food Additives & Contaminants: Part A, 2010Co-Authors: Stefania Somma, Alberto Ritieni, Antonio Logrieco, C Alvarez, V Ricci, L Ferracane, Antonio MorettiAbstract:The importance and widespread incidence of Fusarium poae as a natural contaminant of wheat in different climatic areas warrants investigation into the genetic diversity and toxin profile of a northern Italy population. Eighty-one strains of F. poae isolated from durum wheat kernels, identified by species-specific polymerase chain reaction and translation elongation factor-1alpha gene sequence analysis, were genetically characterized by the amplified fragment length polymorphism (AFLP) technique and analysed by high-pressure liquid chromatography for their ability to produce the beauvericin (BEA) and trichothecene mycotoxins. A high level of variability was observed by using AFLP analyses, with the lowest level of genetic similarity among the strains being approximately 61%. Most of the strains, 95%, produced BEA at
-
Occurrence of Toxic Hexadepsipeptides in Preharvest Maize Ear Rot Infected by Fusarium poae in Poland
Journal of Phytopathology, 2007Co-Authors: Jerzy Chełkowski, Alberto Ritieni, H. Wiśniewska, Giuseppina Mulè, Antonio LogriecoAbstract:Twenty-seven preharvest maize ears affected by Fusarium poae rot (disease score 36-100%) were selected in 1998 and 1999 in Poland and examined for the occurrence of toxic hexadepsipeptides: beauvericin (BEA), enniatin A, enniatin B and enniatin B 1 The identification of F. poae was confirmed by sequence analysis of variable internal transcribed spacer regions and compared with NCBI gene bank DNA sequences. Chemical analyses were performed by HPLC-MS. In 27 ears infected by F. poae were detected: BEA (trace to 46 μg/g) in 18 samples, enniatin A (trace to 37 μg/g) in nine samples, enniatin B (trace to 47 μg/g) in 15 samples and enniatin B, (trace to 25 μg/g) in 12 samples. When 20 strains of F. poae isolated from these samples were cultured on rice, all produced BEA (1.9-75 μg/g), three enniatin A (1.8-2μg/g), 12 enniatin B (1.1-5.1 μg/g) and eight enniatin B 1 (1.2-5.2 μg/g). Occurrence and quantification of enniatin A, enniatin B and enniatin B 1 and their co-occurrence with BEA in maize kernels is reported for the first time.
-
Response evaluation of an E-nose towards contaminated wheat by Fusarium poae fungi
Sensors and Actuators B-chemical, 2006Co-Authors: D.s. Presicce, A. Forleo, Antonella M. Taurino, M. Zuppa, Pietro Siciliano, Barbara Laddomada, Antonio Logrieco, A. ViscontiAbstract:Cereals contaminated by toxigenic fungi can be analysed by detecting and quantifying the related mycotoxins by using complex extraction procedures and analytical techniques. In this work, we studied the complex headspace of wheat samples contaminated by Fusarium poae fungi by SPME-GC-MS, searching the presence of non-grain volatile metabolites due to fungi contamination and their temporal evolution after inoculation. The chemical information on the headspace composition of fungi-contaminated wheat samples were used for the analysis by an electronic nose, evaluating its discrimination ability among differently contaminated samples.
Sebastian Alberto Stenglein - One of the best experts on this subject based on the ideXlab platform.
-
Effects of Fusarium graminearum and Fusarium poae on disease parameters, grain quality and mycotoxins contamination in bread wheat (Part I)
Journal of the Science of Food and Agriculture, 2019Co-Authors: Mauro Martinez, María Inés Dinolfo, D Ramirez Albuquerque, Fernando Biganzoli, Virginia Fernández Pinto, Sebastian Alberto StengleinAbstract:BACKGROUND Wheat is the most important winter crop in the world, being affected by the presence of fungal, mainly those belonging to the Fusarium genus. Fusarium head blight (FHB) is a serious disease that causes important economic damage and quantitative/qualitative losses, with Fusarium graminearum and Fusarium poae being two of the most isolated species worldwide. The present study aimed to evaluate the interaction between F. graminearum and F. poae and the effects on disease parameters, grain quality and mycotoxin contamination on five wheat genotypes under field conditions during three growing seasons. RESULTS Statistical differences between Fusarium treatments were found for disease parameters, grain quality and mycotoxin contamination during the 2014/2015 growing season. High values of incidence (58.00 ± 8.00%), severity (6.28 ± 1.51%) and FHB index (4.72 ± 1.35) were observed for F. graminearum + F. poae treatment. Regarding grain quality, the results showed that the degradation of different protein fractions depends on each Fusarium species: glutenins were degraded preferably by F. graminearum (-70.82%), gliadins were degraded preferably by F. poae (-29.42%), whereas both protein fractions were degraded when both Fusarium species were present (-60.91% and -16.51%, respectively). Significant differences were observed for mycotoxin contamination between genotypes, with Proteo being the most affected (DON = 12.01 ± 3.67 μg g-1 ). In addition, we report that 3-ADON predominated over 15-ADON in the three seasons evaluated. CONCLUSION Variations in plant-pathogen interaction (Fusarium-wheat pathosystem) should be considered at least in years with favorable climatic conditions for FHB development, as a result of the potential impact of this disease on grain quality and mycotoxin contamination. © 2019 Society of Chemical Industry.
-
effects of waterlogging stress on plant pathogen interaction between Fusarium poae and wheat barley
Acta Scientiarum-agronomy, 2019Co-Authors: Mauro Martinez, Sebastian Alberto Stenglein, Agustin Francisco Arata, Laura Lazaro, María Inés DinolfoAbstract:Waterlogging stress is one of the abiotic factors which causes damage to crops affecting yield components and grain quality of wheat and barley. On the other hand, Fusarium poae is one of the most common Fusarium species isolated from wheat and barley. The aim of this study was to evaluate the effects of waterlogging and F. poae on disease parameters, yield components and grain quality of durum and bread wheat and barley. The experiment was carried out using pots under greenhouse conditions. Four treatments were applied: control/control (W0F0), control/ F. poae (W0F1), waterlogging/control (W1F0) and waterlogging/ F. poae (W1F1). The results showed that incidence, severity and FHB index of F. poae were higher in W0F1 compared to W1F1 suggesting that waterlogging treatment would be generating no favorable conditions for fungal growth. Therefore, yield components and grain composition and quality were significantly affected by the Fusarium presence and waterlogging treatment which could induce changes in parameters mainly related to the industrial quality of wheat and barley. These results highlight the behavior of wheat and barley under the combination of abiotic and biotic stress.
-
Resistance of Fusarium poae in Arabidopsis leaves requires mainly functional JA and ET signaling pathways
Fungal Biology, 2017Co-Authors: María Inés Dinolfo, Eliana Castañares, Sebastian Alberto StengleinAbstract:Fusarium poae has been considered as a minor species among those that cause the FHB disease but in recent years several researchers have documented a high frequency of occurrence in several crops. We evaluated the ability of F. poae to produce symptoms in A. thaliana leaves. Moreover, we analyzed the defense of A. thaliana against F. poae using SA, JA, and ET mutants and we monitored the expression level of genes involved in the main signaling pathways related to plant defense. Symptoms were observed in the inoculated leaves demonstrating the ability of F. poae to infect A. thaliana leaves. Moreover, the npr1-1 mutants presented low symptoms compared to Col-0, etr2-1, and coi1-1 and that the coi1-1 mutant was the most susceptible genotypes followed by etr2-1 genotypes. The RT-PCR revealed that PDF1.2, CHI/PR3, and ERF1, three important JA-ET responsive genes and NPR1 and PR1, which are regulated by SA signaling, were expressed upon F. poae inoculation. Our results suggest that JA and ET could play a key role in Arabidopsis leaves defense against F. poae representing the first evaluation of the response of the main A. thaliana phytohormones involved in plant defense in the presence of F. poae.
-
Characterization of a Fusarium poae world-wide collection by using molecular markers
European Journal of Plant Pathology, 2014Co-Authors: María Inés Dinolfo, Eliana Castañares, Sebastian Alberto StengleinAbstract:Fusarium poae has been considered as a minor species among those that cause Fusarium Head Blight (FHB) disease but in recent years several researchers have documented a high frequency of occurrence of this species. In this study, a total of 173 F. poae isolates from Argentina, Belgium, Canada, England, Finland, France, Germany, Hungary, Italy, Luxembourg, Poland, Switzerland and Uruguay were evaluated by using inter simple sequence repeats (ISSR) and amplified fragment length polymorphism (AFLP) to evaluate genetic variability within F. poae and to amplify MAT idiomorphs as a possible mechanism that could explain part of the variability found in this species. The molecular analysis obtained from both molecular markers showed a high intraspecific variability. However, a partial clustering between F. poae isolates and their geographic origin was obtained by ISSR markers while AFLP showed isolates from different geographic locations distributed throughout the dendrogram. Moreover, ISSR grouped all the F. poae isolates into a different cluster from the F. langsethiae and F. sporotrichioides isolates used as outgroups compared with the dendrogram obtained using AFLP markers. Analysis of molecular variance (AMOVA) indicated a high genetic variability in the F. poae collection, with most of the genetic variability resulting from differences within, rather than between, American and European populations by using both molecular markers. Regarding MAT idiomorphs, for most F. poae isolates both MAT-1 and MAT-2 were present from each isolate.
-
Fusarium poae Pathogenicity and Mycotoxin Accumulation on Selected Wheat and Barley Genotypes at a Single Location in Argentina
Plant Disease, 2014Co-Authors: Sebastian Alberto Stenglein, María Inés Dinolfo, Germán Barros, Fabricio Bongiorno, Sofia Noemi Chulze, Maria Virginia MorenoAbstract:Abstract Fusarium poae is a relatively weak pathogen with increasing importance in cereal grains, principally due to its capacity to produce several mycotoxins. In this study, we evaluated the pathogenicity and toxin accumulation of individual F. poae isolates on wheat and barley under natural conditions for 3 years. Analysis of variance demonstrated significant differences for year–genotype, year–isolate, genotype–isolate, and year–genotype–isolate interactions for both incidence and disease severity. Based on contrast analysis, ‘Apogee’ was more susceptible than the other wheat genotypes, wheat genotypes were more susceptible than barley genotypes, durum wheat genotypes were more susceptible than bread wheat genotypes, and barley genotype ‘Scarlett’ had greater symptom development per spike than the other barley genotypes. Neither HT-2 nor T-2 toxins were detected in the grain samples. However, high levels of nivalenol were found in both wheat and barley samples. The increased reported isolation of F. p...
Hailong Zhang - One of the best experts on this subject based on the ideXlab platform.
-
two novel relative double stranded rna mycoviruses infecting Fusarium poae strain sx63
International Journal of Molecular Sciences, 2016Co-Authors: Luan Wang, Jingze Zhang, Hailong ZhangAbstract:Two novel double-stranded RNA (dsRNA) mycoviruses, termed Fusarium poae dsRNA virus 2 (FpV2) and Fusarium poae dsRNA virus 3 (FpV3), were isolated from the plant pathogenic fungus, Fusarium poae strain SX63, and molecularly characterized. FpV2 and FpV3, with respective genome sequences of 9518 and 9419 base pairs (bps), are both predicted to contain two discontinuous open reading frames (ORFs), ORF1 and ORF2. A hypothetical polypeptide (P1) and a RNA-dependent RNA polymerase (RdRp) are encoded by ORF1 and ORF2, respectively. Phytoreo_S7 domain (pfam07236) homologs were detected downstream of the RdRp domain (RdRp_4; pfam02123) of the ORF2-coded proteins of both FpV2 and FpV3. The same shifty heptamers (GGAAAAC) were both found immediately before the stop codon UAG of ORF1 in FpV2 and FpV3, which could mediate programmed –1 ribosomal frameshifting (–1 PRF). Phylogenetic analysis based on RdRp sequences clearly place FpV2 and FpV3 in a taxonomically unassigned dsRNA mycovirus group. Together, with a comparison of genome organization, a new taxonomic family termed Fusagraviridae is proposed to be created to include FpV2- and FpV3-related dsRNA mycoviruses, within which FpV2 and FpV3 would represent two distinct virus species.
