The Experts below are selected from a list of 291 Experts worldwide ranked by ideXlab platform
Farhad Valizadegan - One of the best experts on this subject based on the ideXlab platform.
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The effects of hippocampal opioidergic and septal GABAergic System interactions on anxiety-like behavior in rats.
Life Sciences, 2011Co-Authors: Ghorbangol Ashabi, Shahrbanoo Oryan, Ramesh Ahmadi, Farhad ValizadeganAbstract:Abstract Aims Previous studies have shown that the septum and hippocampus are connected and play a key role in anxiety-related behavior. In the present study, the effects of the interactions between the opioidergic System in the dorsal hippocampus (CA1) and the GABAergic System in the medial septum on anxiety in male Wistar rats were investigated. Main methods An elevated plus maze was used to study the effects of anxiogenic and anxiolytic drugs in rodents. Male Wistar rats were used for this test. We injected morphine (2.5, 5.0, and 7.5 μg/rat) into the CA1 and GABAA agonist muscimol (2.5, 5.0, and 10.0 ng/rat) into the intra-medial septum (MS). Conversely, we detected the microinjection of bicuculline, a GABAA antagonist (10, 20, and 30 ng/rat), into the MS. OAT%, OAE% and locomotor activity were determined by this behavioral test. Key findings We found that the simultaneous administration of intra-CA1 morphine (2.5 μg/rat) and intra-MS muscimol (2.5 ng/rat) increased the magnitude of anxiolysis. On the other hand, simultaneous administration of intra-CA1 morphine (7.5 μg/rat) and intra-MS bicuculline decreased the anxiolytic effect. Significance In conclusion, our data suggest that the opioidergic System in the CA1 is involved in anxiety-related behavior and influences the GABAergic System within the MS. In addition, we observed that the interactions between the opioidergic and GABAergic Systems within the septohippocampus increased anxiety-related behavior compared to stimulation of these receptor Systems independently.
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The effects of hippocampal opioidergic and septal GABAergic System interactions on anxiety-like behavior in rats.
Life sciences, 2011Co-Authors: Ghorbangol Ashabi, Shahrbanoo Oryan, Ramesh Ahmadi, Farhad ValizadeganAbstract:Previous studies have shown that the septum and hippocampus are connected and play a key role in anxiety-related behavior. In the present study, the effects of the interactions between the opioidergic System in the dorsal hippocampus (CA1) and the GABAergic System in the medial septum on anxiety in male Wistar rats were investigated. An elevated plus maze was used to study the effects of anxiogenic and anxiolytic drugs in rodents. Male Wistar rats were used for this test. We injected morphine (2.5, 5.0, and 7.5 μg/rat) into the CA1 and GABA(A) agonist muscimol (2.5, 5.0, and 10.0 ng/rat) into the intra-medial septum (MS). Conversely, we detected the microinjection of bicuculline, a GABA(A) antagonist (10, 20, and 30 ng/rat), into the MS. OAT%, OAE% and locomotor activity were determined by this behavioral test. We found that the simultaneous administration of intra-CA1 morphine (2.5 μg/rat) and intra-MS muscimol (2.5 ng/rat) increased the magnitude of anxiolysis. On the other hand, simultaneous administration of intra-CA1 morphine (7.5 μg/rat) and intra-MS bicuculline decreased the anxiolytic effect. In conclusion, our data suggest that the opioidergic System in the CA1 is involved in anxiety-related behavior and influences the GABAergic System within the MS. In addition, we observed that the interactions between the opioidergic and GABAergic Systems within the septohippocampus increased anxiety-related behavior compared to stimulation of these receptor Systems independently. Crown Copyright © 2011. Published by Elsevier Inc. All rights reserved.
Wei Liu - One of the best experts on this subject based on the ideXlab platform.
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bmscs transplantation improves cognitive impairment via up regulation of hippocampal GABAergic System in a rat model of chronic cerebral hypoperfusion
Neuroscience, 2015Co-Authors: Q Long, Yue Hei, Q Luo, Y Tian, J Yang, Lichun Wei, Wei LiuAbstract:Bone marrow mesenchymal stem cells (BMSCs) transplantation can ameliorate cognitive impairment in chronic ischemic brain injury, but the underlying mechanism is poorly understood. It is considered that the hippocampus holds the capabilities of memory consolidation and spatial navigation, and the gamma amino butyric acid (GABA)ergic System plays an important role in the control of learning and memory processes. Herein, we investigated whether transplantation of BMSCs could improve cognitive impairment via regulating the hippocampal GABAergic System in a rat model of chronic cerebral hypoperfusion. Animals treated with permanent bilateral occlusion of the common carotid arteries (two-vessel occlusion, 2VO) (a rat model of chronic cerebral hypoperfusion) received intravenous injections of BMSCs or saline as experimental group and control group I, the sham-operated rats received intravenous injections of BMSCs or saline as the sham group and control group II. Four weeks later, the Morris Water Maze was employed to evaluate the cognitive changes of each group, immunohistochemistry and western blotting was used to investigate the GABAergic System expression including GABA, glutamic acid decarboxylase 67 (GAD67) or GABA(B) receptor 1 (GABA(B)R1) in the hippocampus. Our results showed that the 2VO model presented decreased capacities of learning and memory and down-regulated the expression of GABA, GAD67 or GABA(B)R1 in the hippocampal CA1 subfield in comparison to the sham group (P<0.05), while administration of BMSCs (experimental group) manifested increased performances of learning sessions and probe tasks, as well as up-regulated expression of GABA, GAD67 or GABA(B)R1 compared with the control group I (P<0.05). Collectively, these findings suggest that transplantation of BMSCs is capable of improving cognitive impairment via up-regulating the hippocampal GABAergic System in a rat model of chronic cerebral hypoperfusion. Hence, BMSCs transplantation could serve as an important tool for cell therapy in chronic cerebral hypoperfusion disorders.
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BMSCs transplantation improves cognitive impairment via up-regulation of hippocampal GABAergic System in a rat model of chronic cerebral hypoperfusion
Neuroscience, 2015Co-Authors: Q Long, Yue Hei, Q Luo, Y Tian, J Yang, Lichun Wei, Wei LiuAbstract:Bone marrow mesenchymal stem cells (BMSCs) transplantation can ameliorate cognitive impairment in chronic ischemic brain injury, but the underlying mechanism is poorly understood. It is considered that the hippocampus holds the capabilities of memory consolidation and spatial navigation, and the gamma amino butyric acid (GABA)ergic System plays an important role in the control of learning and memory processes. Herein, we investigated whether transplantation of BMSCs could improve cognitive impairment via regulating the hippocampal GABAergic System in a rat model of chronic cerebral hypoperfusion. Animals treated with permanent bilateral occlusion of the common carotid arteries (two-vessel occlusion, 2VO) (a rat model of chronic cerebral hypoperfusion) received intravenous injections of BMSCs or saline as experimental group and control group I, the sham-operated rats received intravenous injections of BMSCs or saline as the sham group and control group II. Four weeks later, the Morris Water Maze was employed to evaluate the cognitive changes of each group, immunohistochemistry and western blotting was used to investigate the GABAergic System expression including GABA, glutamic acid decarboxylase 67 (GAD67) or GABA(B) receptor 1 (GABA(B)R1) in the hippocampus. Our results showed that the 2VO model presented decreased capacities of learning and memory and down-regulated the expression of GABA, GAD67 or GABA(B)R1 in the hippocampal CA1 subfield in comparison to the sham group (P
Shahrbanoo Oryan - One of the best experts on this subject based on the ideXlab platform.
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Interaction between Angiotensinergic System and GABAergic System on Thirst in Adult Male Rats
Journal of Behavioral and Brain Science, 2012Co-Authors: Marzieh Shirazi-nejad, Nasser Naghdi, Shahrbanoo OryanAbstract:Thirst is a subjective perception that provides the urge for human and animals to drink fluids and it is important for maintaining body fluid homeostasis and may arise from deficits in either intracellular or extracellular fluid volume. Gamma-aminobutyric acid (GABA) and Angiotensin (Ang) receptors in the brain are involved with thirst, water intake and balance of body liquid. The present study investigated the interaction between Angiotensinergic and GABAergic Systems on water intake in adult male rats. Intracerebroventricular (i.c.v.) injections were carried out in all experiments after 24 h deprivation of water intake. After deprivation the volume of consumed water was measured for 1 h. Administration of Losartan (45 μg/rat), Muscimol (0.1 μg/rat) significantly decreased water intake while, i.c.v. microinjection of Bicuculline (1 μg/rat) significantly increased it as compared to Saline-treated controls. I.c.v. microinjection of Muscimol 15 min after Losartan administration decreased water intake significantly, while, i.c.v. microinjection of Bicuculline 15 min after Losartan administration could attenuate increasing effect of Bicuculline on water intake. It is concluded that Angiotensinergic System have interaction with GABAergic System on water intake.
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The effects of hippocampal opioidergic and septal GABAergic System interactions on anxiety-like behavior in rats.
Life Sciences, 2011Co-Authors: Ghorbangol Ashabi, Shahrbanoo Oryan, Ramesh Ahmadi, Farhad ValizadeganAbstract:Abstract Aims Previous studies have shown that the septum and hippocampus are connected and play a key role in anxiety-related behavior. In the present study, the effects of the interactions between the opioidergic System in the dorsal hippocampus (CA1) and the GABAergic System in the medial septum on anxiety in male Wistar rats were investigated. Main methods An elevated plus maze was used to study the effects of anxiogenic and anxiolytic drugs in rodents. Male Wistar rats were used for this test. We injected morphine (2.5, 5.0, and 7.5 μg/rat) into the CA1 and GABAA agonist muscimol (2.5, 5.0, and 10.0 ng/rat) into the intra-medial septum (MS). Conversely, we detected the microinjection of bicuculline, a GABAA antagonist (10, 20, and 30 ng/rat), into the MS. OAT%, OAE% and locomotor activity were determined by this behavioral test. Key findings We found that the simultaneous administration of intra-CA1 morphine (2.5 μg/rat) and intra-MS muscimol (2.5 ng/rat) increased the magnitude of anxiolysis. On the other hand, simultaneous administration of intra-CA1 morphine (7.5 μg/rat) and intra-MS bicuculline decreased the anxiolytic effect. Significance In conclusion, our data suggest that the opioidergic System in the CA1 is involved in anxiety-related behavior and influences the GABAergic System within the MS. In addition, we observed that the interactions between the opioidergic and GABAergic Systems within the septohippocampus increased anxiety-related behavior compared to stimulation of these receptor Systems independently.
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The effects of hippocampal opioidergic and septal GABAergic System interactions on anxiety-like behavior in rats.
Life sciences, 2011Co-Authors: Ghorbangol Ashabi, Shahrbanoo Oryan, Ramesh Ahmadi, Farhad ValizadeganAbstract:Previous studies have shown that the septum and hippocampus are connected and play a key role in anxiety-related behavior. In the present study, the effects of the interactions between the opioidergic System in the dorsal hippocampus (CA1) and the GABAergic System in the medial septum on anxiety in male Wistar rats were investigated. An elevated plus maze was used to study the effects of anxiogenic and anxiolytic drugs in rodents. Male Wistar rats were used for this test. We injected morphine (2.5, 5.0, and 7.5 μg/rat) into the CA1 and GABA(A) agonist muscimol (2.5, 5.0, and 10.0 ng/rat) into the intra-medial septum (MS). Conversely, we detected the microinjection of bicuculline, a GABA(A) antagonist (10, 20, and 30 ng/rat), into the MS. OAT%, OAE% and locomotor activity were determined by this behavioral test. We found that the simultaneous administration of intra-CA1 morphine (2.5 μg/rat) and intra-MS muscimol (2.5 ng/rat) increased the magnitude of anxiolysis. On the other hand, simultaneous administration of intra-CA1 morphine (7.5 μg/rat) and intra-MS bicuculline decreased the anxiolytic effect. In conclusion, our data suggest that the opioidergic System in the CA1 is involved in anxiety-related behavior and influences the GABAergic System within the MS. In addition, we observed that the interactions between the opioidergic and GABAergic Systems within the septohippocampus increased anxiety-related behavior compared to stimulation of these receptor Systems independently. Crown Copyright © 2011. Published by Elsevier Inc. All rights reserved.
Ghorbangol Ashabi - One of the best experts on this subject based on the ideXlab platform.
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The effects of hippocampal opioidergic and septal GABAergic System interactions on anxiety-like behavior in rats.
Life Sciences, 2011Co-Authors: Ghorbangol Ashabi, Shahrbanoo Oryan, Ramesh Ahmadi, Farhad ValizadeganAbstract:Abstract Aims Previous studies have shown that the septum and hippocampus are connected and play a key role in anxiety-related behavior. In the present study, the effects of the interactions between the opioidergic System in the dorsal hippocampus (CA1) and the GABAergic System in the medial septum on anxiety in male Wistar rats were investigated. Main methods An elevated plus maze was used to study the effects of anxiogenic and anxiolytic drugs in rodents. Male Wistar rats were used for this test. We injected morphine (2.5, 5.0, and 7.5 μg/rat) into the CA1 and GABAA agonist muscimol (2.5, 5.0, and 10.0 ng/rat) into the intra-medial septum (MS). Conversely, we detected the microinjection of bicuculline, a GABAA antagonist (10, 20, and 30 ng/rat), into the MS. OAT%, OAE% and locomotor activity were determined by this behavioral test. Key findings We found that the simultaneous administration of intra-CA1 morphine (2.5 μg/rat) and intra-MS muscimol (2.5 ng/rat) increased the magnitude of anxiolysis. On the other hand, simultaneous administration of intra-CA1 morphine (7.5 μg/rat) and intra-MS bicuculline decreased the anxiolytic effect. Significance In conclusion, our data suggest that the opioidergic System in the CA1 is involved in anxiety-related behavior and influences the GABAergic System within the MS. In addition, we observed that the interactions between the opioidergic and GABAergic Systems within the septohippocampus increased anxiety-related behavior compared to stimulation of these receptor Systems independently.
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The effects of hippocampal opioidergic and septal GABAergic System interactions on anxiety-like behavior in rats.
Life sciences, 2011Co-Authors: Ghorbangol Ashabi, Shahrbanoo Oryan, Ramesh Ahmadi, Farhad ValizadeganAbstract:Previous studies have shown that the septum and hippocampus are connected and play a key role in anxiety-related behavior. In the present study, the effects of the interactions between the opioidergic System in the dorsal hippocampus (CA1) and the GABAergic System in the medial septum on anxiety in male Wistar rats were investigated. An elevated plus maze was used to study the effects of anxiogenic and anxiolytic drugs in rodents. Male Wistar rats were used for this test. We injected morphine (2.5, 5.0, and 7.5 μg/rat) into the CA1 and GABA(A) agonist muscimol (2.5, 5.0, and 10.0 ng/rat) into the intra-medial septum (MS). Conversely, we detected the microinjection of bicuculline, a GABA(A) antagonist (10, 20, and 30 ng/rat), into the MS. OAT%, OAE% and locomotor activity were determined by this behavioral test. We found that the simultaneous administration of intra-CA1 morphine (2.5 μg/rat) and intra-MS muscimol (2.5 ng/rat) increased the magnitude of anxiolysis. On the other hand, simultaneous administration of intra-CA1 morphine (7.5 μg/rat) and intra-MS bicuculline decreased the anxiolytic effect. In conclusion, our data suggest that the opioidergic System in the CA1 is involved in anxiety-related behavior and influences the GABAergic System within the MS. In addition, we observed that the interactions between the opioidergic and GABAergic Systems within the septohippocampus increased anxiety-related behavior compared to stimulation of these receptor Systems independently. Crown Copyright © 2011. Published by Elsevier Inc. All rights reserved.
Q Long - One of the best experts on this subject based on the ideXlab platform.
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bmscs transplantation improves cognitive impairment via up regulation of hippocampal GABAergic System in a rat model of chronic cerebral hypoperfusion
Neuroscience, 2015Co-Authors: Q Long, Yue Hei, Q Luo, Y Tian, J Yang, Lichun Wei, Wei LiuAbstract:Bone marrow mesenchymal stem cells (BMSCs) transplantation can ameliorate cognitive impairment in chronic ischemic brain injury, but the underlying mechanism is poorly understood. It is considered that the hippocampus holds the capabilities of memory consolidation and spatial navigation, and the gamma amino butyric acid (GABA)ergic System plays an important role in the control of learning and memory processes. Herein, we investigated whether transplantation of BMSCs could improve cognitive impairment via regulating the hippocampal GABAergic System in a rat model of chronic cerebral hypoperfusion. Animals treated with permanent bilateral occlusion of the common carotid arteries (two-vessel occlusion, 2VO) (a rat model of chronic cerebral hypoperfusion) received intravenous injections of BMSCs or saline as experimental group and control group I, the sham-operated rats received intravenous injections of BMSCs or saline as the sham group and control group II. Four weeks later, the Morris Water Maze was employed to evaluate the cognitive changes of each group, immunohistochemistry and western blotting was used to investigate the GABAergic System expression including GABA, glutamic acid decarboxylase 67 (GAD67) or GABA(B) receptor 1 (GABA(B)R1) in the hippocampus. Our results showed that the 2VO model presented decreased capacities of learning and memory and down-regulated the expression of GABA, GAD67 or GABA(B)R1 in the hippocampal CA1 subfield in comparison to the sham group (P<0.05), while administration of BMSCs (experimental group) manifested increased performances of learning sessions and probe tasks, as well as up-regulated expression of GABA, GAD67 or GABA(B)R1 compared with the control group I (P<0.05). Collectively, these findings suggest that transplantation of BMSCs is capable of improving cognitive impairment via up-regulating the hippocampal GABAergic System in a rat model of chronic cerebral hypoperfusion. Hence, BMSCs transplantation could serve as an important tool for cell therapy in chronic cerebral hypoperfusion disorders.
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BMSCs transplantation improves cognitive impairment via up-regulation of hippocampal GABAergic System in a rat model of chronic cerebral hypoperfusion
Neuroscience, 2015Co-Authors: Q Long, Yue Hei, Q Luo, Y Tian, J Yang, Lichun Wei, Wei LiuAbstract:Bone marrow mesenchymal stem cells (BMSCs) transplantation can ameliorate cognitive impairment in chronic ischemic brain injury, but the underlying mechanism is poorly understood. It is considered that the hippocampus holds the capabilities of memory consolidation and spatial navigation, and the gamma amino butyric acid (GABA)ergic System plays an important role in the control of learning and memory processes. Herein, we investigated whether transplantation of BMSCs could improve cognitive impairment via regulating the hippocampal GABAergic System in a rat model of chronic cerebral hypoperfusion. Animals treated with permanent bilateral occlusion of the common carotid arteries (two-vessel occlusion, 2VO) (a rat model of chronic cerebral hypoperfusion) received intravenous injections of BMSCs or saline as experimental group and control group I, the sham-operated rats received intravenous injections of BMSCs or saline as the sham group and control group II. Four weeks later, the Morris Water Maze was employed to evaluate the cognitive changes of each group, immunohistochemistry and western blotting was used to investigate the GABAergic System expression including GABA, glutamic acid decarboxylase 67 (GAD67) or GABA(B) receptor 1 (GABA(B)R1) in the hippocampus. Our results showed that the 2VO model presented decreased capacities of learning and memory and down-regulated the expression of GABA, GAD67 or GABA(B)R1 in the hippocampal CA1 subfield in comparison to the sham group (P
