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Sanjay P. Govindwar - One of the best experts on this subject based on the ideXlab platform.
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monitoring the gradual biodegradation of dyes in a simulated textile effluent and development of a novel triple layered fixed bed reactor using a bacterium yeast consortium
Chemical Engineering Journal, 2017Co-Authors: Tatoba R. Waghmode, Byonghun Jeon, Mayur B Kurade, Swapnil M Patil, Sanjay P. GovindwarAbstract:Abstract Textile industry effluents contain a variety of dyes, which are normally resistant to biodegradation. A bacterial-yeast consortium ( Brevibacillus laterosporus and Galactomyces geotrichum ) was used for decolorization of two real textile effluents (RTE) and a simulated synthetic effluent (SSE). It showed enhanced decolorization compared to that of individual microorganisms with decolorization efficiency of 89, 60 and 69% for RTE-1, RTE-2 and SSE respectively, within 48 h. The cumulative action of oxido-reductive enzyme in the consortium was responsible for improved decolorization. Spectroscopic analysis suggested effective biodegradation of dyes present in the SSE by the consortium contrarily to the individual strains. The gradual biodegradation of each dye present in the SSE was monitored using high performance thin layer chromatography (HPTLC). The consortium biodegraded all of the dyes within 1 h as compared to that of partial biodegradation by the individual microorganisms. A novel, triple layered fixed bed reactor was designed for continuous decolorization of effluent. It showed >80% decolorization (at 100 mL h −1 flow-rate), for a period of 7 days, along with ∼78% reduction in COD. The reproducibility of the bioreactor could be maintained for three consecutive cycles (7 days each).
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biodegradation of rubine gfl by Galactomyces geotrichum mtcc 1360 and subsequent toxicological analysis by using cytotoxicity genotoxicity and oxidative stress studies
Microbiology, 2012Co-Authors: Tatoba R. Waghmode, Akhil N Kabra, Mayur B Kurade, Sanjay P. GovindwarAbstract:Galactomyces geotrichum MTCC 1360 showed 87 % decolorization of the azo dye Rubine GFL (50 mg l−1) within 96 h at 30 °C and pH 7.0 under static conditions, with significant reduction of chemical oxygen demand (67 %) and total organic carbon (59 %). Examination of oxidoreductive enzymes, namely laccase, tyrosinase and azo reductase, confirmed their role in decolorization and degradation of Rubine GFL. Biodegradation of Rubine GFL into different metabolites was confirmed using high-performance TLC, HPLC, Fourier transform IR spectroscopy and GC-MS analysis. During toxicological studies, cell death was observed in Rubine GFL-treated Allium cepa root cells. Toxicological studies before and after microbial treatment were done with respect to cytotoxicity, genotoxicity, oxidative stress, antioxidant enzyme status, protein oxidation and lipid peroxidation using root cells of A. cepa. The analysis with A. cepa showed that the dye exerts oxidative stress and subsequently has a toxic effect on the root cells, whereas its metabolites are less toxic. Phytotoxicity studies revealed the less toxic nature of the metabolites as compared with Rubine GFL.
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time dependent degradation of mixture of structurally different azo and non azo dyes by using Galactomyces geotrichum mtcc 1360
International Biodeterioration & Biodegradation, 2011Co-Authors: Tatoba R. Waghmode, Mayur B Kurade, Sanjay P. GovindwarAbstract:Abstract Galactomyces geotrichum MTCC 1360, a yeast species showed 88% ADMI (American dye manufacturing institute) removal of mixture of structurally different dyes (Remazol red, Golden yellow HER, Rubine GFL, Scarlet RR, Methyl red, Brown 3 REL, Brilliant blue) (70 mg l−1) within 24 h at 30 °C and pH 7.0 under shaking condition (120 rpm). Glucose (0.5%) as a carbon source was found to be more effective than other sources used. The medium with metal salt (CaCl2, ZnSO4, FeCl3, MgCl2, CuSO4) (0.5 mM) showed less ADMI removal as compared to control, but did not inhibit complete decolorization. The presence of tyrosinase, NADH-DCIP reductase and induction in laccase activity during decolorization indicated their role in degradation. HPTLC (High performance thin layer chromatography) analysis revealed the removal of individual dyes at different time intervals from dye mixture, indicating preferential degradation of dyes. FTIR (Fourier transform infrared spectroscopy) and HPLC (High performance liquid chromatography) analysis of samples before and after decolorization confirmed the biotransformation of dye. The reduction of COD (Chemical oxygen demand) (69%), TOC (Total organic carbon) (43%), and phytotoxicity study indicated the conversion of complex dye molecules into simpler oxidizable products having less toxic nature.
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degradation and detoxification of disperse dye scarlet rr by Galactomyces geotrichum mtcc 1360
Journal of Microbiology and Biotechnology, 2009Co-Authors: S. U. Jadhav, Dhawal P Tamboli, Gajanan Ghodake, Amar A Telke, Sanjay P. GovindwarAbstract:oC, respectively. Enzymatic studies revealed an induction of the enzymes, including flavin reductase during the initial stage and lignin peroxidase after complete decolorization of the dye. Decolorization of the dye was induced by the addition of CaCO3 to the medium. EDTA had an inhibitory effect on the dye decolorization along with the laccase activity. The metabolites formed after complete decolorization were analyzed by UV-VIS, HPLC, and FTIR. The GC/MS identification of 3 H quinazolin-4one, 2-ethylamino-acetamide, 1-chloro-4-nitro-benzene, N(4-chloro-phenyl)-hydroxylamine, and 4-chloro-pheny-lamine as the final metabolites corroborated with the degradation of Scarlet RR. The phytotoxicity study revealed the nontoxic nature of the final metabolites. A possible degradation pathway is suggested to understand the mechanism used by G. geotrichum and thereby aiding development of technologies for the application of this organism to the cleaning-up of aquatic and terrestrial environments.
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biodegradation of methyl red by Galactomyces geotrichum mtcc 1360
International Biodeterioration & Biodegradation, 2008Co-Authors: S. U. Jadhav, Satish Kalme, Sanjay P. GovindwarAbstract:Abstract Galactomyces geotrichum MTCC 1360 can decolorize triphenylmethane, azo and reactive high exhaust textile dyes. At shaking condition this strain showed 100% decolorization of a toxic azo dye Methyl red (100 m gl −1 ) within 1 h in deionized water at 30 °C. The degradation of Methyl red was possible through a broad pH (3–12) and temperature (5–50 °C) range. Glucose and mycelium concentration had increased the decolorization rate, but the addition of 1 gl −1 molasses in deionized water made decolorization possible in only 10 min. Induction in the NADH–dichloro phenol indophenol (NADH–DCIP) reductase, Malachite green reductase, laccase and lignin peroxidase (Lip) activities were observed in the cells obtained after complete decolorization, showing that there is direct involvement in the degradation of Methyl red. The absence of N - N ′-dimethyl-p-phenylenediamine (DMPD) in 5 °C, 2-aminobenzoic acid (ABA) in 50 °C and both the compounds in 30 °C sample have shown the differences in the metabolic fate of Methyl red at different temperatures. The untreated dye at 300 mg l −1 concentration showed 88% germination inhibition in Sorghum bicolor , whereas it was 72% in Triticum aestivum . There was no germination inhibition for both the plants by Methyl red metabolites at 300 mg l −1 concentration. The scientific relevance of the paper The azo dye Methyl red (100 mg l −1 ) was decolorized by G. geotrichum MTCC 1360 within 1 h at shaking condition in deionized water. This organism could decolorize Methyl red at wide pH and temperature ranges. Decolorization time was reduced to 10 min by the addition of molasses to deionized water. There was induction in laccase and Lip, NADH–DCIP reductase and Malachite green reductase activities. The metabolic fate of Methyl red changes with temperature which can be evidenced by the formation of 2-ABA at 5 °C, N - N ′-DMPD at 50 °C and both the compounds were absent at 30 °C. Phytotoxicity showed that metabolites of dye had induced shoot and root length of both the tested plants.
Tatoba R. Waghmode - One of the best experts on this subject based on the ideXlab platform.
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monitoring the gradual biodegradation of dyes in a simulated textile effluent and development of a novel triple layered fixed bed reactor using a bacterium yeast consortium
Chemical Engineering Journal, 2017Co-Authors: Tatoba R. Waghmode, Byonghun Jeon, Mayur B Kurade, Swapnil M Patil, Sanjay P. GovindwarAbstract:Abstract Textile industry effluents contain a variety of dyes, which are normally resistant to biodegradation. A bacterial-yeast consortium ( Brevibacillus laterosporus and Galactomyces geotrichum ) was used for decolorization of two real textile effluents (RTE) and a simulated synthetic effluent (SSE). It showed enhanced decolorization compared to that of individual microorganisms with decolorization efficiency of 89, 60 and 69% for RTE-1, RTE-2 and SSE respectively, within 48 h. The cumulative action of oxido-reductive enzyme in the consortium was responsible for improved decolorization. Spectroscopic analysis suggested effective biodegradation of dyes present in the SSE by the consortium contrarily to the individual strains. The gradual biodegradation of each dye present in the SSE was monitored using high performance thin layer chromatography (HPTLC). The consortium biodegraded all of the dyes within 1 h as compared to that of partial biodegradation by the individual microorganisms. A novel, triple layered fixed bed reactor was designed for continuous decolorization of effluent. It showed >80% decolorization (at 100 mL h −1 flow-rate), for a period of 7 days, along with ∼78% reduction in COD. The reproducibility of the bioreactor could be maintained for three consecutive cycles (7 days each).
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decolorization and degradation of xenobiotic azo dye reactive yellow 84a and textile effluent by Galactomyces geotrichum
Chemosphere, 2014Co-Authors: Mayur B Kurade, Tatoba R. Waghmode, Akhil N Kabra, Dhawal P Tamboli, Pil Joo KimAbstract:Abstract Galactomyces geotrichum MTCC 1360 exhibited 86% decolorization of azo dye Reactive Yellow-84A (50 mg L −1 ) within 30 h at 30 °C and pH 7.0 under static condition. Examination of azoreductase, laccase and tyrosinase enzyme activities confirmed their prominent role in Reactive Yellow-84A degradation. Considerable reduction of COD (73%) and TOC (62%) during degradation of the dye was indicative of conversion of complex dye into simple products, which were further analyzed by HPLC, FTIR, GC–MS and HPTLC. The degradation products were identified as 4(5-hydroxy, 4-amino cyclopentane) sulfobenzene and 4(5-hydroxy cyclopentane) sulfobenzene by GC–MS. In addition, when G. geotrichum was applied to decolorize textile effluent, it showed 85% of true color removal (ADMI removal) within 72 h, along with a significant reduction in TOC and COD. Phytotoxicity studies revealed the less toxic nature of degraded Reactive Yellow-84A as compared to original dye.
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biodegradation of rubine gfl by Galactomyces geotrichum mtcc 1360 and subsequent toxicological analysis by using cytotoxicity genotoxicity and oxidative stress studies
Microbiology, 2012Co-Authors: Tatoba R. Waghmode, Akhil N Kabra, Mayur B Kurade, Sanjay P. GovindwarAbstract:Galactomyces geotrichum MTCC 1360 showed 87 % decolorization of the azo dye Rubine GFL (50 mg l−1) within 96 h at 30 °C and pH 7.0 under static conditions, with significant reduction of chemical oxygen demand (67 %) and total organic carbon (59 %). Examination of oxidoreductive enzymes, namely laccase, tyrosinase and azo reductase, confirmed their role in decolorization and degradation of Rubine GFL. Biodegradation of Rubine GFL into different metabolites was confirmed using high-performance TLC, HPLC, Fourier transform IR spectroscopy and GC-MS analysis. During toxicological studies, cell death was observed in Rubine GFL-treated Allium cepa root cells. Toxicological studies before and after microbial treatment were done with respect to cytotoxicity, genotoxicity, oxidative stress, antioxidant enzyme status, protein oxidation and lipid peroxidation using root cells of A. cepa. The analysis with A. cepa showed that the dye exerts oxidative stress and subsequently has a toxic effect on the root cells, whereas its metabolites are less toxic. Phytotoxicity studies revealed the less toxic nature of the metabolites as compared with Rubine GFL.
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time dependent degradation of mixture of structurally different azo and non azo dyes by using Galactomyces geotrichum mtcc 1360
International Biodeterioration & Biodegradation, 2011Co-Authors: Tatoba R. Waghmode, Mayur B Kurade, Sanjay P. GovindwarAbstract:Abstract Galactomyces geotrichum MTCC 1360, a yeast species showed 88% ADMI (American dye manufacturing institute) removal of mixture of structurally different dyes (Remazol red, Golden yellow HER, Rubine GFL, Scarlet RR, Methyl red, Brown 3 REL, Brilliant blue) (70 mg l−1) within 24 h at 30 °C and pH 7.0 under shaking condition (120 rpm). Glucose (0.5%) as a carbon source was found to be more effective than other sources used. The medium with metal salt (CaCl2, ZnSO4, FeCl3, MgCl2, CuSO4) (0.5 mM) showed less ADMI removal as compared to control, but did not inhibit complete decolorization. The presence of tyrosinase, NADH-DCIP reductase and induction in laccase activity during decolorization indicated their role in degradation. HPTLC (High performance thin layer chromatography) analysis revealed the removal of individual dyes at different time intervals from dye mixture, indicating preferential degradation of dyes. FTIR (Fourier transform infrared spectroscopy) and HPLC (High performance liquid chromatography) analysis of samples before and after decolorization confirmed the biotransformation of dye. The reduction of COD (Chemical oxygen demand) (69%), TOC (Total organic carbon) (43%), and phytotoxicity study indicated the conversion of complex dye molecules into simpler oxidizable products having less toxic nature.
Pereira Ermelinda - One of the best experts on this subject based on the ideXlab platform.
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Perfil enzimático de leveduras isoladas durante o processamento de azeitonas de mesa da cultivar Negrinha de Freixo
Associação Portuguesa de Horticultura (APH), 2020Co-Authors: Tatiane Cristina Gonçalves De ,oliveira, Colla Eliane, Pereira J.a., Baptista Paula, Pereira ErmelindaAbstract:No presente trabalho, avaliou-se o perfil enzimático de leveduras isoladas durante o processo de fermentação natural de azeitonas de mesa da cultivar Negrinha de Freixo, a mais importante da região do Nordeste de Portugal. Foram testados onze isolados de leveduras, pertencentes aos géneros Saccharomyces, Candida, Pichia, Debaromyces, Rhodotorula e Galactomyces, através de métodos qualitativos, para a produção de lipase, protease, amilase, xilanase, catalase e β-glucosidase. A atividade da catalase foi observada em todas as espécies isoladas (C. tropicalis, P. membranifaciens, S. cerevesiae, C. norvegica, D. hansenii, R. graminis, C. boidinii, P. guilliermondii, R. glutinis, P. manshurica e G. ressii). A atividade da amilase foi observada apenas em G. ressii. Todas as espécies testadas apresentaram reduzida ou nula atividade para as enzimas xilanase e protease. R. graminis, R. glutinis e G. ressi apresentaram elevada atividade da enzima β-glucosidase e S. cerevisae e P. manshurica elevada atividade da enzima lipase. Os resultados obtidos demonstram o potencial de uso de algumas leveduras para fins biotecnológicos, designadamente como culturas starters durante o processamento de azeitona de mesa.info:eu-repo/semantics/publishedVersio
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Probiotic potential of indigenous yeasts isolated during the fermentation of table olives from Northeast of Portugal
'Elsevier BV', 2017Co-Authors: Tatiane Cristina Gonçalves De ,oliveira, Ramalhosa Elsa, Colla Eliane, Pereira J.a., Nunes Luís, Pereira ErmelindaAbstract:The aim of this study was to evaluate the probiotic potential of 16 yeast strains previously isolated during the natural fermentation of table olives Negrinha de Freixo cultivar, in relation to enzymatic activities; ability to grow at 37 °C; antimicrobial activity; autoaggregation capacity; antioxidant activity and survival in gastrointestinal tract conditions. The highest antioxidant activity was observed for Saccharomyces cerevisiae, similar to the reference strain S. boulardii. Candida norvegica 7A and Galactomyces reessii 34A showed antifungal ability to the pathogenic microorganism Cryptococcus neoformans. Regarding the autoaggregation capacity, S. cerevisiae 15A, C. tropicalis 1A and C. norvegica 7A showed > 80% after 24 h. Pichia guilliermondii 25A and C. norvegica 7A were the most resistant to the simulated digestive conditions, similar to the reference strain (S. boulardii). Thus, these results suggest that some yeast strains involved in the fermentation of table olives have probiotic potential. Industrial relevance In this study, we highlight the probiotic potential of yeast microbiota usually found in green table olives. These yeast strains could be used as culture starters for the development of new functional products.info:eu-repo/semantics/publishedVersio
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Antioxidant activity of indigenous yeasts isolated during the fermentation oftabíe otïves from Northeast of Portugal
2016Co-Authors: Tatiane Cristina Gonçalves De ,oliveira, Ramalhosa Elsa, Colla Eliane, Pereira J.a., Pereira ErmelindaAbstract:Yeasts have an important role in fermented foods, including table olives. These microorganisms can be a new source of natural antioxidants. Free-radical-scavenging antioxidants have potential as protective agents against various degenerative diseases caused by oxidative damage. In the present study, antioxidant activity of indigenous yeasts isolated during the natural fermentation of table olives (Negrinha Freixo cv) was evaluated. These strains had previously been identified by sequencing the ITS region of rDNA. The antioxidant activity was performed by the percentage of reduction of the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical. The studied strains are included in the genera Saccharomyces, Candida, Pichia, Debaryomyces, Rhodotorula and Galactomyces. Saccharomyces boulardii was included as probiotic reference strain. All strains showed distinct antioxidant activity. The ability to scavenge DPPH radical indicated that S. cerevisiae isolated from table olives may be a promising candidate strain for use as probiotics with antioxidant activityinfo:eu-repo/semantics/publishedVersio
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Antioxidant activity of indigenous yeasts isolated during the fermentation of table olives from Northeast of Portugal
2016Co-Authors: Tatiane Cristina Gonçalves De ,oliveira, Ramalhosa Elsa, Colla Eliane, Pereira J.a., Pereira ErmelindaAbstract:Yeasts have an important role in fermented foods, including table olives. These microorganisms can be a new source of natural antioxidants. Free-radical-scavenging antioxidants have potential as protective agents against various degenerative diseases caused by oxidative damage. In the present study, antioxidant activity of indigenous yeasts isolated during the natural fermentation of table olives (Negrinha Freixo cv.) was evaluated. These strains were previously identified by rDNA sequences of the ITS region. The antioxidant activity was performed by the percentage of reduction of the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical. The studied strains are included in the genera Saccharomyces, Candida, Pichia, Debaryomyces, Rhodotorula and Galactomyces. Saccharomyces boulardii was included as probiotic reference strain. All strains showed distinct antioxidant activity. The ability to scavenge DPPH radical indicated that S. cerevisiae isolated from table olives may be a promising candidate strain for use as probiotics with antioxidant activity.info:eu-repo/semantics/publishedVersio
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Perfil enzimático de leveduras isoladas durante o processamento de azeitonas de mesa da cultivar Negrinha de Freixo
2016Co-Authors: Tatiane Cristina Gonçalves De ,oliveira, Colla Eliane, Pereira J.a., Baptista Paula, Pereira ErmelindaAbstract:O presente trabalho pretendeu avaliar o perfil enzimático de leveduras isoladas durante o processo de fermentação natural de azeitonas de mesa da cultivar Negrinha de Freixo, a mais importante da região do Nordeste de Portugal. Foram isoladas e identificadas molecularmente onze leveduras pertencentes aos géneros Saccharomyces, Candida, Pichia, Debaromyces, Rhodotorula e Galactomyces, que posteriormente foram testadas através de métodos qualitativos para a produção das enzimas lipase, protease, amilase, xilanase, catalase e β-glucosidase. A actividade da catalase foi observada em todas as espécies isoladas (C. tropicalis, P. membranifaciens, S. cerevesiae, C. norvegica, D. hansenii, R. graminis, C. boidinii, P. guilliermondii, R. glutinis, P. manshurica e G. ressii). A atividade da amilase foi observada apenas em G. ressii. Todas as espécies testadas apresentaram reduzida ou nula atividade para as enzimas xilanase e protease. R. graminis, R. glutinis e G. ressi apresentaram elevada atividade da enzima β-glucosidase e S. cerevisae e P. manshurica elevada atividade lipolítica. Os resultados obtidos demonstram o potencial de uso de algumas leveduras para fins biotecnológicos, designadamente como culturas starters durante o processamento de azeitona de mesa.info:eu-repo/semantics/publishedVersio
Mayur B Kurade - One of the best experts on this subject based on the ideXlab platform.
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monitoring the gradual biodegradation of dyes in a simulated textile effluent and development of a novel triple layered fixed bed reactor using a bacterium yeast consortium
Chemical Engineering Journal, 2017Co-Authors: Tatoba R. Waghmode, Byonghun Jeon, Mayur B Kurade, Swapnil M Patil, Sanjay P. GovindwarAbstract:Abstract Textile industry effluents contain a variety of dyes, which are normally resistant to biodegradation. A bacterial-yeast consortium ( Brevibacillus laterosporus and Galactomyces geotrichum ) was used for decolorization of two real textile effluents (RTE) and a simulated synthetic effluent (SSE). It showed enhanced decolorization compared to that of individual microorganisms with decolorization efficiency of 89, 60 and 69% for RTE-1, RTE-2 and SSE respectively, within 48 h. The cumulative action of oxido-reductive enzyme in the consortium was responsible for improved decolorization. Spectroscopic analysis suggested effective biodegradation of dyes present in the SSE by the consortium contrarily to the individual strains. The gradual biodegradation of each dye present in the SSE was monitored using high performance thin layer chromatography (HPTLC). The consortium biodegraded all of the dyes within 1 h as compared to that of partial biodegradation by the individual microorganisms. A novel, triple layered fixed bed reactor was designed for continuous decolorization of effluent. It showed >80% decolorization (at 100 mL h −1 flow-rate), for a period of 7 days, along with ∼78% reduction in COD. The reproducibility of the bioreactor could be maintained for three consecutive cycles (7 days each).
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decolorization and degradation of xenobiotic azo dye reactive yellow 84a and textile effluent by Galactomyces geotrichum
Chemosphere, 2014Co-Authors: Mayur B Kurade, Tatoba R. Waghmode, Akhil N Kabra, Dhawal P Tamboli, Pil Joo KimAbstract:Abstract Galactomyces geotrichum MTCC 1360 exhibited 86% decolorization of azo dye Reactive Yellow-84A (50 mg L −1 ) within 30 h at 30 °C and pH 7.0 under static condition. Examination of azoreductase, laccase and tyrosinase enzyme activities confirmed their prominent role in Reactive Yellow-84A degradation. Considerable reduction of COD (73%) and TOC (62%) during degradation of the dye was indicative of conversion of complex dye into simple products, which were further analyzed by HPLC, FTIR, GC–MS and HPTLC. The degradation products were identified as 4(5-hydroxy, 4-amino cyclopentane) sulfobenzene and 4(5-hydroxy cyclopentane) sulfobenzene by GC–MS. In addition, when G. geotrichum was applied to decolorize textile effluent, it showed 85% of true color removal (ADMI removal) within 72 h, along with a significant reduction in TOC and COD. Phytotoxicity studies revealed the less toxic nature of degraded Reactive Yellow-84A as compared to original dye.
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biodegradation of rubine gfl by Galactomyces geotrichum mtcc 1360 and subsequent toxicological analysis by using cytotoxicity genotoxicity and oxidative stress studies
Microbiology, 2012Co-Authors: Tatoba R. Waghmode, Akhil N Kabra, Mayur B Kurade, Sanjay P. GovindwarAbstract:Galactomyces geotrichum MTCC 1360 showed 87 % decolorization of the azo dye Rubine GFL (50 mg l−1) within 96 h at 30 °C and pH 7.0 under static conditions, with significant reduction of chemical oxygen demand (67 %) and total organic carbon (59 %). Examination of oxidoreductive enzymes, namely laccase, tyrosinase and azo reductase, confirmed their role in decolorization and degradation of Rubine GFL. Biodegradation of Rubine GFL into different metabolites was confirmed using high-performance TLC, HPLC, Fourier transform IR spectroscopy and GC-MS analysis. During toxicological studies, cell death was observed in Rubine GFL-treated Allium cepa root cells. Toxicological studies before and after microbial treatment were done with respect to cytotoxicity, genotoxicity, oxidative stress, antioxidant enzyme status, protein oxidation and lipid peroxidation using root cells of A. cepa. The analysis with A. cepa showed that the dye exerts oxidative stress and subsequently has a toxic effect on the root cells, whereas its metabolites are less toxic. Phytotoxicity studies revealed the less toxic nature of the metabolites as compared with Rubine GFL.
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time dependent degradation of mixture of structurally different azo and non azo dyes by using Galactomyces geotrichum mtcc 1360
International Biodeterioration & Biodegradation, 2011Co-Authors: Tatoba R. Waghmode, Mayur B Kurade, Sanjay P. GovindwarAbstract:Abstract Galactomyces geotrichum MTCC 1360, a yeast species showed 88% ADMI (American dye manufacturing institute) removal of mixture of structurally different dyes (Remazol red, Golden yellow HER, Rubine GFL, Scarlet RR, Methyl red, Brown 3 REL, Brilliant blue) (70 mg l−1) within 24 h at 30 °C and pH 7.0 under shaking condition (120 rpm). Glucose (0.5%) as a carbon source was found to be more effective than other sources used. The medium with metal salt (CaCl2, ZnSO4, FeCl3, MgCl2, CuSO4) (0.5 mM) showed less ADMI removal as compared to control, but did not inhibit complete decolorization. The presence of tyrosinase, NADH-DCIP reductase and induction in laccase activity during decolorization indicated their role in degradation. HPTLC (High performance thin layer chromatography) analysis revealed the removal of individual dyes at different time intervals from dye mixture, indicating preferential degradation of dyes. FTIR (Fourier transform infrared spectroscopy) and HPLC (High performance liquid chromatography) analysis of samples before and after decolorization confirmed the biotransformation of dye. The reduction of COD (Chemical oxygen demand) (69%), TOC (Total organic carbon) (43%), and phytotoxicity study indicated the conversion of complex dye molecules into simpler oxidizable products having less toxic nature.
Carlos A Rosa - One of the best experts on this subject based on the ideXlab platform.
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Geotrichum silvicola sp. nov., a novel asexual arthroconidial yeast species related to the genus Galactomyces.
International journal of systematic and evolutionary microbiology, 2005Co-Authors: Raphael S Pimenta, Priscila D D Alves, Ary Corrêa, Marc-andré Lachance, G S Prasad, Rajaram, B R R P Sinha, Carlos A RosaAbstract:Four strains of an asexual arthroconidial yeast species were isolated from Drosophila flies in two Atlantic rain forest sites in Brazil and two strains from oak tasar silkworm larvae (Antheraea proylei) in India. Analysis of the sequences of the D1/D2 large subunit rRNA gene showed that this yeast represented a novel species of the genus Geotrichum, described as Geotrichum silvicola sp. nov. The novel species was related to the ascogenous genus Galactomyces. The closest relatives of Geotrichum silvicola were Galactomyces sp. strain NRRL Y-6418 and Galactomyces geotrichum. The type culture of Geotrichum silvicola is UFMG-354-2T (=CBS 9194T=NRRL Y-27641T).
