The Experts below are selected from a list of 360 Experts worldwide ranked by ideXlab platform
Hafize Dilek Tepe - One of the best experts on this subject based on the ideXlab platform.
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effects of Galium Aparine extract on the cell viability cell cycle and cell death in breast cancer cell lines
Journal of Ethnopharmacology, 2016Co-Authors: Harika Atmaca, Emir Bozkurt, Mustafa Cittan, Hafize Dilek TepeAbstract:Abstract Ethnopharmacological relevance Galium species have been traditionally used for its anti-cancer, antioxidant, anti-inflammatory, antimicrobial and cardioprotective effects in the folk medicine. Galium Aparine (GA) is a typical climbing plant growing widespread in Anatolia. Aim of the study To investigate the potential anti-proliferative and apoptotic effect of GA methanol (MeOH) extract on MCF-7 and MDA-MB-231 human breast cancer cells and MCF-10A untransformed breast epithelial cells. Materials and methods First, the extract was characterized by both liquid chromatography/quadrupole time-of-flight mass spectrometry (LC/Q-TOF/MS) and gas chromatography-mass spectrometry (GC-MS) analyses. Then, cell viability and cell cycle distribution were investigated by XTT assay and PI staining by flow cytometry, respectively. Cell death was determined by Annexin V FITC/7-AAD staining. Results A total of 14 major phytochemicals were identified by LC/Q-TOF/MS and 34 volatile compounds were determined by GC-MS. The extract was cytotoxic in both breast cancer cell lines in a concentration and time dependent manner and showed G1 block after 72 h extract treatment. However, it was not cytotoxic to MCF-10A breast epithelial cells. Flow cytometry analyses revealed that apoptosis was induced in MDA-MB-231 cells; however, necrosis was induced in MCF-7 cells. Conclusion Our study suggests that GA MeOH extract may have potential anti-cancer effects against breast cancer cells without impairing normal breast epithelial cells. Ability to induction of non-apoptotic cell death besides apoptotic cell death by this complex plant-derived mixture may enable the killing of apoptosis resistant breast cancer cells but further studies should be conducted to investigate the bioavailability and metabolism of it in vivo .
A H Cobb - One of the best experts on this subject based on the ideXlab platform.
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the modification of phytosterol profiles and in vitro photosynthetic electron transport of Galium Aparine l cleavers treated with the fungicide epoxiconazole
Plant Growth Regulation, 1997Co-Authors: J M Benton, A H CobbAbstract:Foliar application of the triazole fungicide, epoxiconazole, retarded the growth of Galium Aparine L. (cleavers). GC-MS and GC analysis clearly indicated that phytosterol biosynthesis in stem and leaflet tissue was significantly affected by this treatment. For example, in leaflet tissues, 125 g ai ha-1 (field rate) caused reductions in campesterol and sitosterol of 81percnt; and 75percnt; respectively. C14-methyl phytosterols such as 14agr;-methylergost-8-enol, obtusifoliol and dihydroobtusifoliol were detected in treated tissues indicating that epoxiconazole inhibits the cytochrome P-450 dependent obtusifoliol 14agr;-demethylase. In addition, ratios of campesterol to sitosterol were reduced. Stigmasterol was not detected in control or treated tissues. Preliminary determination of photosynthetic characteristics of isolated thylakoids from treated plants indicated that electron transport and oxygen evolution were impaired by epoxiconazole and these effects were dose-related. Ten days after treatment, oxygen evolution from thylakoids (determined as electron flow from water to ferricyanide) isolated from control plants was 24.2 micro;mol mg-1 chl h-1, whilst treatment with 125 g and 250 g ai ha-1 reduced this rate to 15.2 micro;mol and 8.2 micro;mol mg-1 chl h-1 an inhibition of 37 and 67percnt; respectively. These results suggest that epoxiconazole influences thylakoid integrity and function in addition to phytosterol biosynthesis in G. Aparine.
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the plant growth regulator activity of the fungicide epoxiconazole on Galium Aparine l cleavers
Plant Growth Regulation, 1995Co-Authors: J M Benton, A H CobbAbstract:The plant growth regulator activity of epoxiconazole, a new triazole fungicide, was investigated by time-course, dose-response and histology experiments with Galium Aparine L. (cleavers). Seven days after treatment with 125g ai ha−1 epoxiconazole (field rate), plant height was reduced by 43%. After seventeen days, leaflet area was reduced by 27% but leaflet fresh weight was not significantly influenced. This was partly because leaflet thickness had increased by 20% following epoxiconazole application. Chlorophyll concentrations were also increased on a unit area basis. Examination of leaflet anatomy showed that epoxiconazole elongated palisade, spongy mesophyll and upper epidermal cells. For example, 125g ai ha−1 caused a 35% increase in the length of spongy mesophyll cells. Epoxiconazole also prevented cell separation as there were significantly more palisade and spongy mesophyll cells per unit area than in leaflets sprayed with water. Stem development was reduced and 125g ai ha−1 inhibited the elongation of pith cells in stem tissue by 53%. However, the simultaneous application of gibberellin A3 (GA3) with epoxiconazole resulted in stem elongation similar to that of control plants. These observations are consistent with the expected effects following the inhibition of cytochrome P-450 dependent enzyme activity.
Harika Atmaca - One of the best experts on this subject based on the ideXlab platform.
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effects of Galium Aparine extract on the angiogenic cytokines and erk1 2 proteins in human breast cancer cells
Celal Bayar Universitesi Fen Bilimleri Dergisi, 2017Co-Authors: Harika AtmacaAbstract:Tumor angiogenesis is a multiple step process regulated by a range of pro- and anti-angiogenic factors, cytokines and several signaling cascades, including the ERK1/2 MAP kinase pathway. Galium Aparine (GA) is a perennial plant of the family Rubiaceae, growing widespread in Anatolia. It has been shown that GA methanol (MeOH) extract has anti-proliferative and apoptotic effects on human breast cancer cells. To investigate the underlying mechanisms that might account for the anti-proliferative effect of GA extract, changes in angiogenic cytokines and ERK1/2 proteins were investigated in human MCF-7 and MDA-MB-231 breast cancer cells. Viability of breast cancer cells after treatment of GA were determined by Cell Proliferation Kit II. Changes in angiogenic cytokines in breast cancer cells was done by a Human Angiogenesis Antibody Array. Protein levels of phospho ERK1 and 2 were evaluated by western blot analysis. GA induced cytotoxicity and apoptosis in a time- and concentration-dependent manner in both cancer cells. Dipeptidyl peptidase-4 (DPPIV), insulin-like growth factor-binding protein 1 (IBP-1) and transforming growth factor beta 1 (TGF-β1) levels were significantly decreased; however, secretion of serpin E1 was significantly increased by GA extract in MCF-7 cells. In MDA-MB-231 cells, the levels of amphiregulin (AR), interleukin 8 (IL-8), insulin-like growth factor-binding protein 3 (IBP-3), metalloproteinase-8 (MMP-8), heparin-binding EGF-like growth factor (HB-EGF) and platelet-derived growth factor (PDGF) were decreased by GA extract treatment. The secretion of pro-angiogenic cytokines such as neuregulin-1 (NRG1-β1), vascular endothelial growth factor (VEGF) and tissue factor (TF) were significantly decreased by GA treatment in both breast cancer cells. There was also a significant decrease in protein levels of both ERK1 and ERK2 in MCF-7 and MDA-MB-231 breast cancer cells by GA treatment. These data suggest that GA might be a promising candidate for anti-tumor and anti-angiogenic treatment of breast cancer.
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effects of Galium Aparine extract on the cell viability cell cycle and cell death in breast cancer cell lines
Journal of Ethnopharmacology, 2016Co-Authors: Harika Atmaca, Emir Bozkurt, Mustafa Cittan, Hafize Dilek TepeAbstract:Abstract Ethnopharmacological relevance Galium species have been traditionally used for its anti-cancer, antioxidant, anti-inflammatory, antimicrobial and cardioprotective effects in the folk medicine. Galium Aparine (GA) is a typical climbing plant growing widespread in Anatolia. Aim of the study To investigate the potential anti-proliferative and apoptotic effect of GA methanol (MeOH) extract on MCF-7 and MDA-MB-231 human breast cancer cells and MCF-10A untransformed breast epithelial cells. Materials and methods First, the extract was characterized by both liquid chromatography/quadrupole time-of-flight mass spectrometry (LC/Q-TOF/MS) and gas chromatography-mass spectrometry (GC-MS) analyses. Then, cell viability and cell cycle distribution were investigated by XTT assay and PI staining by flow cytometry, respectively. Cell death was determined by Annexin V FITC/7-AAD staining. Results A total of 14 major phytochemicals were identified by LC/Q-TOF/MS and 34 volatile compounds were determined by GC-MS. The extract was cytotoxic in both breast cancer cell lines in a concentration and time dependent manner and showed G1 block after 72 h extract treatment. However, it was not cytotoxic to MCF-10A breast epithelial cells. Flow cytometry analyses revealed that apoptosis was induced in MDA-MB-231 cells; however, necrosis was induced in MCF-7 cells. Conclusion Our study suggests that GA MeOH extract may have potential anti-cancer effects against breast cancer cells without impairing normal breast epithelial cells. Ability to induction of non-apoptotic cell death besides apoptotic cell death by this complex plant-derived mixture may enable the killing of apoptosis resistant breast cancer cells but further studies should be conducted to investigate the bioavailability and metabolism of it in vivo .
J M Benton - One of the best experts on this subject based on the ideXlab platform.
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the modification of phytosterol profiles and in vitro photosynthetic electron transport of Galium Aparine l cleavers treated with the fungicide epoxiconazole
Plant Growth Regulation, 1997Co-Authors: J M Benton, A H CobbAbstract:Foliar application of the triazole fungicide, epoxiconazole, retarded the growth of Galium Aparine L. (cleavers). GC-MS and GC analysis clearly indicated that phytosterol biosynthesis in stem and leaflet tissue was significantly affected by this treatment. For example, in leaflet tissues, 125 g ai ha-1 (field rate) caused reductions in campesterol and sitosterol of 81percnt; and 75percnt; respectively. C14-methyl phytosterols such as 14agr;-methylergost-8-enol, obtusifoliol and dihydroobtusifoliol were detected in treated tissues indicating that epoxiconazole inhibits the cytochrome P-450 dependent obtusifoliol 14agr;-demethylase. In addition, ratios of campesterol to sitosterol were reduced. Stigmasterol was not detected in control or treated tissues. Preliminary determination of photosynthetic characteristics of isolated thylakoids from treated plants indicated that electron transport and oxygen evolution were impaired by epoxiconazole and these effects were dose-related. Ten days after treatment, oxygen evolution from thylakoids (determined as electron flow from water to ferricyanide) isolated from control plants was 24.2 micro;mol mg-1 chl h-1, whilst treatment with 125 g and 250 g ai ha-1 reduced this rate to 15.2 micro;mol and 8.2 micro;mol mg-1 chl h-1 an inhibition of 37 and 67percnt; respectively. These results suggest that epoxiconazole influences thylakoid integrity and function in addition to phytosterol biosynthesis in G. Aparine.
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the plant growth regulator activity of the fungicide epoxiconazole on Galium Aparine l cleavers
Plant Growth Regulation, 1995Co-Authors: J M Benton, A H CobbAbstract:The plant growth regulator activity of epoxiconazole, a new triazole fungicide, was investigated by time-course, dose-response and histology experiments with Galium Aparine L. (cleavers). Seven days after treatment with 125g ai ha−1 epoxiconazole (field rate), plant height was reduced by 43%. After seventeen days, leaflet area was reduced by 27% but leaflet fresh weight was not significantly influenced. This was partly because leaflet thickness had increased by 20% following epoxiconazole application. Chlorophyll concentrations were also increased on a unit area basis. Examination of leaflet anatomy showed that epoxiconazole elongated palisade, spongy mesophyll and upper epidermal cells. For example, 125g ai ha−1 caused a 35% increase in the length of spongy mesophyll cells. Epoxiconazole also prevented cell separation as there were significantly more palisade and spongy mesophyll cells per unit area than in leaflets sprayed with water. Stem development was reduced and 125g ai ha−1 inhibited the elongation of pith cells in stem tissue by 53%. However, the simultaneous application of gibberellin A3 (GA3) with epoxiconazole resulted in stem elongation similar to that of control plants. These observations are consistent with the expected effects following the inhibition of cytochrome P-450 dependent enzyme activity.
Jawaid Ahmed Zai - One of the best experts on this subject based on the ideXlab platform.
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evaluation of diverse antioxidant activities of Galium Aparine
Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 2013Co-Authors: Jasia Bokhari, Muhammad Rashid Khan, Maria Shabbir, Umbreen Rashid, Shumaila Jan, Jawaid Ahmed ZaiAbstract:Methanol extract and its n-hexane, ethyl acetate, butanol and aqueous fraction of Galium Aparine L. (Rubiacea) were evaluated in vitro for their antioxidant capacity (DPPH, superoxide radical, phosphomolybdate assay); reducing power (ABTS, hydroxyl, hydrogen peroxide, to reduce Fe(3+) to Fe(2+) ions) and to estimate total flavonoid and phenolic contents. All the free radical generating assay models depicted differential positive scavenging activity but considerable magnitude for all the fractions. The results showed that aqueous fraction strongly scavenge the DPPH, ABTS, hydroxyl, hydrogen peroxide and superoxide radicals. A significantly high correlation coefficient existed between IC(50) values of DPPH and superoxide radical with total phenolic content and phosphomolybdate assay with total flavonoid contents, respectively. These results suggested that aqueous fraction can be a good source of antioxidant therapeutic in oxidative stress damages.
