The Experts below are selected from a list of 87 Experts worldwide ranked by ideXlab platform
Hao Wu - One of the best experts on this subject based on the ideXlab platform.
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antibacterial activity and mechanism of a laccase catalyzed chitosan Gallic Acid Derivative against escherichia coli and staphylococcus aureus
Food Control, 2019Co-Authors: Kangjing Li, Guilin Guan, Hao WuAbstract:Abstract In this paper, the antimicrobial activity and mechanism of a laccase-catalyzed chitosan–Gallic Acid Derivative were evaluated. The results showed that compared to the parent chitosan, the Derivative could significantly inhibit the growth of Escherichia coli and Staphylococcus aureus, wherein the latter was more sensitive to the Derivative than the former. The chitosan–Gallic Acid Derivative could disrupt the cell membrane of E. coli and S. aureus, resulting in leakage of cytoplasm and an increase in relative conductivity. The morphological changes were manifested by the aggregation of bacterial cells and formation of an irregular shape. Agarose gel electrophoresis showed that chitosan Derivatives could enter bacterial cells through damaged cell membranes and inhibit DNA synthesis in the nucleus. In addition, the cytotoxicity was significantly reduced by proper modification of chitosan with Gallic Acid. The above results indicated that there was a great potential for laccase-catalyzed chitosan–Gallic Acid Derivative for use as a food preservative.
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Antibacterial activity and mechanism of a laccase-catalyzed chitosan–Gallic Acid Derivative against Escherichia coli and Staphylococcus aureus
Food Control, 2019Co-Authors: Kangjing Li, Guilin Guan, Hao WuAbstract:Abstract In this paper, the antimicrobial activity and mechanism of a laccase-catalyzed chitosan–Gallic Acid Derivative were evaluated. The results showed that compared to the parent chitosan, the Derivative could significantly inhibit the growth of Escherichia coli and Staphylococcus aureus, wherein the latter was more sensitive to the Derivative than the former. The chitosan–Gallic Acid Derivative could disrupt the cell membrane of E. coli and S. aureus, resulting in leakage of cytoplasm and an increase in relative conductivity. The morphological changes were manifested by the aggregation of bacterial cells and formation of an irregular shape. Agarose gel electrophoresis showed that chitosan Derivatives could enter bacterial cells through damaged cell membranes and inhibit DNA synthesis in the nucleus. In addition, the cytotoxicity was significantly reduced by proper modification of chitosan with Gallic Acid. The above results indicated that there was a great potential for laccase-catalyzed chitosan–Gallic Acid Derivative for use as a food preservative.
Kangjing Li - One of the best experts on this subject based on the ideXlab platform.
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antibacterial activity and mechanism of a laccase catalyzed chitosan Gallic Acid Derivative against escherichia coli and staphylococcus aureus
Food Control, 2019Co-Authors: Kangjing Li, Guilin Guan, Hao WuAbstract:Abstract In this paper, the antimicrobial activity and mechanism of a laccase-catalyzed chitosan–Gallic Acid Derivative were evaluated. The results showed that compared to the parent chitosan, the Derivative could significantly inhibit the growth of Escherichia coli and Staphylococcus aureus, wherein the latter was more sensitive to the Derivative than the former. The chitosan–Gallic Acid Derivative could disrupt the cell membrane of E. coli and S. aureus, resulting in leakage of cytoplasm and an increase in relative conductivity. The morphological changes were manifested by the aggregation of bacterial cells and formation of an irregular shape. Agarose gel electrophoresis showed that chitosan Derivatives could enter bacterial cells through damaged cell membranes and inhibit DNA synthesis in the nucleus. In addition, the cytotoxicity was significantly reduced by proper modification of chitosan with Gallic Acid. The above results indicated that there was a great potential for laccase-catalyzed chitosan–Gallic Acid Derivative for use as a food preservative.
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Antibacterial activity and mechanism of a laccase-catalyzed chitosan–Gallic Acid Derivative against Escherichia coli and Staphylococcus aureus
Food Control, 2019Co-Authors: Kangjing Li, Guilin Guan, Hao WuAbstract:Abstract In this paper, the antimicrobial activity and mechanism of a laccase-catalyzed chitosan–Gallic Acid Derivative were evaluated. The results showed that compared to the parent chitosan, the Derivative could significantly inhibit the growth of Escherichia coli and Staphylococcus aureus, wherein the latter was more sensitive to the Derivative than the former. The chitosan–Gallic Acid Derivative could disrupt the cell membrane of E. coli and S. aureus, resulting in leakage of cytoplasm and an increase in relative conductivity. The morphological changes were manifested by the aggregation of bacterial cells and formation of an irregular shape. Agarose gel electrophoresis showed that chitosan Derivatives could enter bacterial cells through damaged cell membranes and inhibit DNA synthesis in the nucleus. In addition, the cytotoxicity was significantly reduced by proper modification of chitosan with Gallic Acid. The above results indicated that there was a great potential for laccase-catalyzed chitosan–Gallic Acid Derivative for use as a food preservative.
Guilin Guan - One of the best experts on this subject based on the ideXlab platform.
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antibacterial activity and mechanism of a laccase catalyzed chitosan Gallic Acid Derivative against escherichia coli and staphylococcus aureus
Food Control, 2019Co-Authors: Kangjing Li, Guilin Guan, Hao WuAbstract:Abstract In this paper, the antimicrobial activity and mechanism of a laccase-catalyzed chitosan–Gallic Acid Derivative were evaluated. The results showed that compared to the parent chitosan, the Derivative could significantly inhibit the growth of Escherichia coli and Staphylococcus aureus, wherein the latter was more sensitive to the Derivative than the former. The chitosan–Gallic Acid Derivative could disrupt the cell membrane of E. coli and S. aureus, resulting in leakage of cytoplasm and an increase in relative conductivity. The morphological changes were manifested by the aggregation of bacterial cells and formation of an irregular shape. Agarose gel electrophoresis showed that chitosan Derivatives could enter bacterial cells through damaged cell membranes and inhibit DNA synthesis in the nucleus. In addition, the cytotoxicity was significantly reduced by proper modification of chitosan with Gallic Acid. The above results indicated that there was a great potential for laccase-catalyzed chitosan–Gallic Acid Derivative for use as a food preservative.
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Antibacterial activity and mechanism of a laccase-catalyzed chitosan–Gallic Acid Derivative against Escherichia coli and Staphylococcus aureus
Food Control, 2019Co-Authors: Kangjing Li, Guilin Guan, Hao WuAbstract:Abstract In this paper, the antimicrobial activity and mechanism of a laccase-catalyzed chitosan–Gallic Acid Derivative were evaluated. The results showed that compared to the parent chitosan, the Derivative could significantly inhibit the growth of Escherichia coli and Staphylococcus aureus, wherein the latter was more sensitive to the Derivative than the former. The chitosan–Gallic Acid Derivative could disrupt the cell membrane of E. coli and S. aureus, resulting in leakage of cytoplasm and an increase in relative conductivity. The morphological changes were manifested by the aggregation of bacterial cells and formation of an irregular shape. Agarose gel electrophoresis showed that chitosan Derivatives could enter bacterial cells through damaged cell membranes and inhibit DNA synthesis in the nucleus. In addition, the cytotoxicity was significantly reduced by proper modification of chitosan with Gallic Acid. The above results indicated that there was a great potential for laccase-catalyzed chitosan–Gallic Acid Derivative for use as a food preservative.
Suresh Kumar Karri - One of the best experts on this subject based on the ideXlab platform.
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amelioration of carbon tetrachloride induced hepatotoxicity by β glucogallin a Gallic Acid Derivative of emblica officinalis gaertn euphorbiaceae
International journal of scientific research, 2016Co-Authors: Muhammed Majeed, Kalyanam Nagabhushanam, Beena Bhat, Anurag Pande, Shaheen Majeed, Suresh Kumar KarriAbstract:For the first time we evaluated hepatoprotective activity of aqueous fruit extracts of E. officinalis standardized for 10% β-Glucogallin in female Wistar rats. Animals were divided into 6 groups, 6 rats in each. Hepatotoxicity was induced using carbon tetrachloride (CCl 4 ) in Group 2 to 6, while Group 1 served as normal control. Animals in group 2, 3 and 4 were administered the test substance orally with different doses (300, 600, 1200 mg/kg body weight), 3 h after treatment with CCl 4, once daily for 10 days. Group 5 received standard drug (Silymarin 25 mg/kg), Group 6 was left untreated (negative control). A suite of ORAC (oxygen radical absorbance capacity) assayswere also performed invitro to test the antioxidant potential of β–Glucogallin.Group 2 animals that received a dosage of 300mg/kg body weight of extract showed statistically significant (p<0.01) changes in the serum antioxidant markers, transaminases and liver cells recovery from CCl 4 toxicity, confirmed by histopathological studies. It is concluded that hepatoprotective activity of aqueous amla fruit extract was due to the presence of Gallic Acid Derivatives like β-Glucogallin, and may not be ascribed to ascorbic Acid alone.
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Amelioration of Carbon Tetrachloride Induced Hepatotoxicity by Β-Glucogallin, a Gallic Acid Derivative of Emblica Officinalis Gaertn.(Euphorbiaceae)
International journal of scientific research, 2016Co-Authors: Muhammed Majeed, Kalyanam Nagabhushanam, Beena Bhat, Anurag Pande, Shaheen Majeed, Suresh Kumar KarriAbstract:For the first time we evaluated hepatoprotective activity of aqueous fruit extracts of E. officinalis standardized for 10% β-Glucogallin in female Wistar rats. Animals were divided into 6 groups, 6 rats in each. Hepatotoxicity was induced using carbon tetrachloride (CCl 4 ) in Group 2 to 6, while Group 1 served as normal control. Animals in group 2, 3 and 4 were administered the test substance orally with different doses (300, 600, 1200 mg/kg body weight), 3 h after treatment with CCl 4, once daily for 10 days. Group 5 received standard drug (Silymarin 25 mg/kg), Group 6 was left untreated (negative control). A suite of ORAC (oxygen radical absorbance capacity) assayswere also performed invitro to test the antioxidant potential of β–Glucogallin.Group 2 animals that received a dosage of 300mg/kg body weight of extract showed statistically significant (p
Tomomasa Yano - One of the best experts on this subject based on the ideXlab platform.
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chemotherapeutic potential of two Gallic Acid Derivative compounds from leaves of casearia sylvestris sw flacourtiaceae
European Journal of Pharmacology, 2009Co-Authors: Saulo L Da Silva, Jamal Da Silva Chaar, Tomomasa YanoAbstract:Abstract Casearia sylvestris is a plant used in the treatment of several diseases, including cancer. Studies have shown that C. sylvestris presents an interesting antitumoral potential, due to the presence of casearins and some sesquiterpens with antitumoral activity. In this work, we tested the potential chemotherapeutic of two Gallic Acid-derived compounds isolated from C. sylvestris leaves: isobutyl gallate-3,5-dimethyl ether (IGDE) and methyl gallate-3,5-dimethyl ether (MGDE). We utilized two tumoral models: Ehrlich ascites tumor cells (EAT)/BALB/c mice and Lewis lung cancer cells (LLC1)/C57bl/6 mice. MGDE and IGDE increased the survival of mice inoculated with EAT cells and decreased the tumor volume in the LLC1 model, compared to control groups. Both compounds presented similar and low in vitro cytotoxicity against Ehrlich ascites tumor cells and did not present any significant toxicity against Lewis lung cancer cells. Since the direct in vitro activity against Ehrlich tumor and Lewis lung cancer cells was low, we investigated the effects of MGDE or IGDE treatment on the activity of total natural killer cells from Ehrlich ascites tumor-bearing mice, as a possible explanation for the mechanisms of these compounds in vivo. MGDE and IGDE improved NK cell cytotoxicity against Ehrlich ascites cells. As expected, tumor growth in non-treated mice markedly suppressed NK cell cytolysis while, IGDE completely reversed this effect, when mice were treated with 0.5 mg/kg dosages of these compounds for 4 days. The pharmacokinetic studies showed that IGDE remains in the organism for a long period of time, possibly explaining the higher compound efficiency.
