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Sergio Sanchez - One of the best experts on this subject based on the ideXlab platform.
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The new allelic variant of the subtilase cytotoxin (subAB2) is common among Shiga toxin-producing Escherichia coli strains from large Game Animals and their meat and meat products.
Veterinary microbiology, 2013Co-Authors: Sergio Sanchez, María Teresa Llorente, Silvia Herrera-león, Remigio Martínez, Sandra Díaz-sánchez, Dolors VidalAbstract:Subtilase cytotoxin (SubAB) is an AB5 toxin produced by Shiga toxin (Stx)-producing Escherichia coli (STEC) strains usually lacking the eae gene product intimin. Two allelic variants of SubAB encoding genes have been described: subAB1, located on a plasmid, and subAB2, located on a pathogenicity island (PAI) together with tia gene. While subAB1 has been reported to be more frequent among bovine strains, subAB2 has been mainly associated with strains from small ruminants. We investigated the presence of the two variants of subAB among 59 eae-negative STEC from large Game Animals (deer and wild boar) and their meat and meat products in order to assess the role of other species in the epidemiology of subAB-positive, eae-negative STEC. For this approach, the strains were PCR-screened for the presence of subAB, including the specific detection of both allelic variants, for the presence of saa, tia and sab, and for stx subtyping. Overall, subAB genes were detected in 71.2% of the strains: 84.1% of the strains from deer and 33.3% of the strains from wild boar. Most of them (97.6%) possessed subAB2 and most of these subAB2-positive strains (92.7%) were also positive for tia and negative for saa, suggesting the presence of the subAB2-harbouring PAI. Subtype stx2b was present in most of the strains (67.8%) and a statistically significant association could be established between subAB2 and stx2b. Our results suggest that large Game Animals, mainly deer, may represent an important animal reservoir of subAB2-positive, eae-negative STEC, and also highlight the risk of human infection posed by the consumption of large Game meat and meat products.
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prevalence of shiga toxin producing escherichia coli salmonella spp and campylobacter spp in large Game Animals intended for consumption relationship with management practices and livestock influence
Veterinary Microbiology, 2013Co-Authors: Sandra Diazsanchez, Sergio Sanchez, Rafael Mateo, Silvia Herreraleon, Concepcion Porrero, Juan Blanco, Ghizlane Dahbi, Jesus E Blanco, Azucena Mora, Irene HanningAbstract:Although wild ruminants have been identified as reservoirs of Shiga-toxin producing Escherichia coli (STEC), little information is available concerning the role of Salmonella spp. and Campylobacter spp. in large Game species. We evaluated the presence of these pathogens in faeces (N=574) and carcasses (N=585) sampled from red deer (N=295), wild boar (N=333) and other ungulates (fallow deer, mouflon) (N=9). Animal sampling was done in situ from 33 hunting estates during two hunting seasons. Salmonella spp. and Campylobacter spp. strains associated with human campylobacteriosis were infrequently detected indicating that both pathogens had a limited zoonotic risk in our study area. The overall STEC prevalence in Animals was 21% (134/637), being significantly higher in faeces from red deer (90 out of 264). A total of 58 isolates were serotyped. Serotypes O146:H- and O27:H30 were the most frequent in red deer and the majority of isolates from red deer and wild boar were from serotypes previously found in STEC strains associated with human infection, including the serotype O157:H7. The STEC prevalence in red deer faeces was significantly higher with the presence of livestock (p<0, 01) where high densities of red deer (p<0.001) were present. To the best of our knowledge, this is the first study reporting the occurrence of Salmonella spp. and STEC in carcasses of large Game Animals. Furthermore, this study confirmed by pulsed-field gel electrophoresis (PFGE) that cross contamination of STEC during carcass dressing occurred, implying the likelihood of these pathogens entering into the food chain.
Matthias Contzen - One of the best experts on this subject based on the ideXlab platform.
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reliable differentiation of a non toxigenic tox gene bearing corynebacterium ulcerans variant frequently isolated from Game Animals using maldi tof ms
Veterinary Microbiology, 2019Co-Authors: Tobias Eisenberg, Peter Kutzer, Andreas Sing, Martin Peters, Anja Berger, Heimo Lassnig, Helmut Hotzel, R Sting, Matthias ContzenAbstract:Abstract Corynebacterium (C.) ulcerans is a zoonotic member of the C. diphtheriae group and is known to cause abscesses in humans and several animal species. Toxigenic strains, expressing the tox gene encoding diphtheria toxin, are also able to cause diphtheria in humans. In recent years, a n on- t oxigenic but t ox gene- b earing (NTTB) variant of C. ulcerans has been identified that was frequently isolated from clinically healthy as well as from diseased wildlife Animals, especially wild boars (Sus scrofa scrofa) in Germany and Austria. The described clinical cases showed similar signs of disease and the isolated corynebacteria displayed common genetic features as well as similar spectroscopic characteristics, therefore being assigned to a so called wild boar cluster (WBC). This study describes the establishment and validation of a method using MALDI-TOF mass spectrometry for a reliable differentiation between various members of the C. diphtheriae group at species level as well as a reliable sub-level identification of C. ulcerans isolates of the WBC variant. For this study 93 C. ulcerans isolates from wildlife Animals, 41 C. ulcerans isolates from other Animals and humans, and 53 isolates from further representatives of the C. diphtheriae group, as well as 26 non-diphtheriae group Corynebacteria collected via the MALDI user platform from seven MALDI users were used. By assigning 86 C. ulcerans isolates to the WBC the extensive geographical distribution of this previously less noticed variant in two Central European countries could be shown.
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nontoxigenic tox bearing corynebacterium ulcerans infection among Game Animals germany
Emerging Infectious Diseases, 2014Co-Authors: Tobias Eisenberg, Andreas Sing, Peter Kutzer, Martin Peters, Matthias ContzenAbstract:Corynebacterium ulcerans may cause diphtheria in humans and caseous lymphadenitis in Animals. We isolated nontoxigenic tox-bearing C. ulcerans from 13 Game Animals in Germany. Our results indicate a role for Game Animals as reservoirs for zoonotic C. ulcerans.
Irene Hanning - One of the best experts on this subject based on the ideXlab platform.
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prevalence of shiga toxin producing escherichia coli salmonella spp and campylobacter spp in large Game Animals intended for consumption relationship with management practices and livestock influence
Veterinary Microbiology, 2013Co-Authors: Sandra Diazsanchez, Sergio Sanchez, Rafael Mateo, Silvia Herreraleon, Concepcion Porrero, Juan Blanco, Ghizlane Dahbi, Jesus E Blanco, Azucena Mora, Irene HanningAbstract:Although wild ruminants have been identified as reservoirs of Shiga-toxin producing Escherichia coli (STEC), little information is available concerning the role of Salmonella spp. and Campylobacter spp. in large Game species. We evaluated the presence of these pathogens in faeces (N=574) and carcasses (N=585) sampled from red deer (N=295), wild boar (N=333) and other ungulates (fallow deer, mouflon) (N=9). Animal sampling was done in situ from 33 hunting estates during two hunting seasons. Salmonella spp. and Campylobacter spp. strains associated with human campylobacteriosis were infrequently detected indicating that both pathogens had a limited zoonotic risk in our study area. The overall STEC prevalence in Animals was 21% (134/637), being significantly higher in faeces from red deer (90 out of 264). A total of 58 isolates were serotyped. Serotypes O146:H- and O27:H30 were the most frequent in red deer and the majority of isolates from red deer and wild boar were from serotypes previously found in STEC strains associated with human infection, including the serotype O157:H7. The STEC prevalence in red deer faeces was significantly higher with the presence of livestock (p<0, 01) where high densities of red deer (p<0.001) were present. To the best of our knowledge, this is the first study reporting the occurrence of Salmonella spp. and STEC in carcasses of large Game Animals. Furthermore, this study confirmed by pulsed-field gel electrophoresis (PFGE) that cross contamination of STEC during carcass dressing occurred, implying the likelihood of these pathogens entering into the food chain.
Dolors Vidal - One of the best experts on this subject based on the ideXlab platform.
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The new allelic variant of the subtilase cytotoxin (subAB2) is common among Shiga toxin-producing Escherichia coli strains from large Game Animals and their meat and meat products.
Veterinary microbiology, 2013Co-Authors: Sergio Sanchez, María Teresa Llorente, Silvia Herrera-león, Remigio Martínez, Sandra Díaz-sánchez, Dolors VidalAbstract:Subtilase cytotoxin (SubAB) is an AB5 toxin produced by Shiga toxin (Stx)-producing Escherichia coli (STEC) strains usually lacking the eae gene product intimin. Two allelic variants of SubAB encoding genes have been described: subAB1, located on a plasmid, and subAB2, located on a pathogenicity island (PAI) together with tia gene. While subAB1 has been reported to be more frequent among bovine strains, subAB2 has been mainly associated with strains from small ruminants. We investigated the presence of the two variants of subAB among 59 eae-negative STEC from large Game Animals (deer and wild boar) and their meat and meat products in order to assess the role of other species in the epidemiology of subAB-positive, eae-negative STEC. For this approach, the strains were PCR-screened for the presence of subAB, including the specific detection of both allelic variants, for the presence of saa, tia and sab, and for stx subtyping. Overall, subAB genes were detected in 71.2% of the strains: 84.1% of the strains from deer and 33.3% of the strains from wild boar. Most of them (97.6%) possessed subAB2 and most of these subAB2-positive strains (92.7%) were also positive for tia and negative for saa, suggesting the presence of the subAB2-harbouring PAI. Subtype stx2b was present in most of the strains (67.8%) and a statistically significant association could be established between subAB2 and stx2b. Our results suggest that large Game Animals, mainly deer, may represent an important animal reservoir of subAB2-positive, eae-negative STEC, and also highlight the risk of human infection posed by the consumption of large Game meat and meat products.
Tobias Eisenberg - One of the best experts on this subject based on the ideXlab platform.
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reliable differentiation of a non toxigenic tox gene bearing corynebacterium ulcerans variant frequently isolated from Game Animals using maldi tof ms
Veterinary Microbiology, 2019Co-Authors: Tobias Eisenberg, Peter Kutzer, Andreas Sing, Martin Peters, Anja Berger, Heimo Lassnig, Helmut Hotzel, R Sting, Matthias ContzenAbstract:Abstract Corynebacterium (C.) ulcerans is a zoonotic member of the C. diphtheriae group and is known to cause abscesses in humans and several animal species. Toxigenic strains, expressing the tox gene encoding diphtheria toxin, are also able to cause diphtheria in humans. In recent years, a n on- t oxigenic but t ox gene- b earing (NTTB) variant of C. ulcerans has been identified that was frequently isolated from clinically healthy as well as from diseased wildlife Animals, especially wild boars (Sus scrofa scrofa) in Germany and Austria. The described clinical cases showed similar signs of disease and the isolated corynebacteria displayed common genetic features as well as similar spectroscopic characteristics, therefore being assigned to a so called wild boar cluster (WBC). This study describes the establishment and validation of a method using MALDI-TOF mass spectrometry for a reliable differentiation between various members of the C. diphtheriae group at species level as well as a reliable sub-level identification of C. ulcerans isolates of the WBC variant. For this study 93 C. ulcerans isolates from wildlife Animals, 41 C. ulcerans isolates from other Animals and humans, and 53 isolates from further representatives of the C. diphtheriae group, as well as 26 non-diphtheriae group Corynebacteria collected via the MALDI user platform from seven MALDI users were used. By assigning 86 C. ulcerans isolates to the WBC the extensive geographical distribution of this previously less noticed variant in two Central European countries could be shown.
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nontoxigenic tox bearing corynebacterium ulcerans infection among Game Animals germany
Emerging Infectious Diseases, 2014Co-Authors: Tobias Eisenberg, Andreas Sing, Peter Kutzer, Martin Peters, Matthias ContzenAbstract:Corynebacterium ulcerans may cause diphtheria in humans and caseous lymphadenitis in Animals. We isolated nontoxigenic tox-bearing C. ulcerans from 13 Game Animals in Germany. Our results indicate a role for Game Animals as reservoirs for zoonotic C. ulcerans.
