The Experts below are selected from a list of 15072 Experts worldwide ranked by ideXlab platform
Joanne L Parrott - One of the best experts on this subject based on the ideXlab platform.
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oil sands tailings pond sediment toxicity to early life stages of northern pike esox lucius
Science of The Total Environment, 2018Co-Authors: Jason C Raine, Leszek Romanowski, Daniel Turcotte, Joanne L ParrottAbstract:Abstract The Athabasca River in Alberta flows through natural sources of eroding oil sands bitumen and oil sands mining operations that may result in low level contamination of surface waters. Northern pike (Esox lucius) are apex predators and important food and Game Fish species native to the Athabasca River system. This species has the potential to be exposed to both natural and anthropogenic sources of contamination from oil sands related materials throughout its life cycle. Pike are difficult to rear in the laboratory and little information exists on the toxicity of oil sands related materials to this key indigenous Fish species. In this study, the potential effects of two sediment samples collected from different areas of one tailings pond in the Athabasca oil sands area are assessed in a daily renewal bioassay on early life stages of northern pike. Gametes were collected from spawning wild pike captured from a reference site outside of the oil sands area. Fertilized eggs were exposed to control water or increasing concentrations of tailings pond sediments for 21 days, coinciding with initiation of exogenous feeding and completion of yolk absorption. Developing Fish were examined for survival and changes in body weight, length, and development. Embryos exhibited increased developmental abnormalities and decreased growth and survival with increasing sediment concentration. Both sediment samples had similar levels of naphthenic acids and similar types of PAHs, with alkylated PAHs dominating. However, concentrations of total and alkylated PAHs differed between sediment samples and were related to increasing developmental abnormalities and decreased growth and survival. This is consistent with developmental changes observed with exposure to PAHs in other Fish species. These results provide information on the effects of tailings pond sediments comprising mixtures of PAHs and alkylated PAHs on the development and survival of a key species in the northern aquatic ecosystem.
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oil sands tailings pond sediment toxicity to early life stages of northern pike esox lucius
Science of The Total Environment, 2018Co-Authors: Jason C Raine, Leszek Romanowski, Daniel Turcotte, Joanne L ParrottAbstract:Abstract The Athabasca River in Alberta flows through natural sources of eroding oil sands bitumen and oil sands mining operations that may result in low level contamination of surface waters. Northern pike (Esox lucius) are apex predators and important food and Game Fish species native to the Athabasca River system. This species has the potential to be exposed to both natural and anthropogenic sources of contamination from oil sands related materials throughout its life cycle. Pike are difficult to rear in the laboratory and little information exists on the toxicity of oil sands related materials to this key indigenous Fish species. In this study, the potential effects of two sediment samples collected from different areas of one tailings pond in the Athabasca oil sands area are assessed in a daily renewal bioassay on early life stages of northern pike. Gametes were collected from spawning wild pike captured from a reference site outside of the oil sands area. Fertilized eggs were exposed to control water or increasing concentrations of tailings pond sediments for 21 days, coinciding with initiation of exogenous feeding and completion of yolk absorption. Developing Fish were examined for survival and changes in body weight, length, and development. Embryos exhibited increased developmental abnormalities and decreased growth and survival with increasing sediment concentration. Both sediment samples had similar levels of naphthenic acids and similar types of PAHs, with alkylated PAHs dominating. However, concentrations of total and alkylated PAHs differed between sediment samples and were related to increasing developmental abnormalities and decreased growth and survival. This is consistent with developmental changes observed with exposure to PAHs in other Fish species. These results provide information on the effects of tailings pond sediments comprising mixtures of PAHs and alkylated PAHs on the development and survival of a key species in the northern aquatic ecosystem.
Jason C Raine - One of the best experts on this subject based on the ideXlab platform.
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oil sands tailings pond sediment toxicity to early life stages of northern pike esox lucius
Science of The Total Environment, 2018Co-Authors: Jason C Raine, Leszek Romanowski, Daniel Turcotte, Joanne L ParrottAbstract:Abstract The Athabasca River in Alberta flows through natural sources of eroding oil sands bitumen and oil sands mining operations that may result in low level contamination of surface waters. Northern pike (Esox lucius) are apex predators and important food and Game Fish species native to the Athabasca River system. This species has the potential to be exposed to both natural and anthropogenic sources of contamination from oil sands related materials throughout its life cycle. Pike are difficult to rear in the laboratory and little information exists on the toxicity of oil sands related materials to this key indigenous Fish species. In this study, the potential effects of two sediment samples collected from different areas of one tailings pond in the Athabasca oil sands area are assessed in a daily renewal bioassay on early life stages of northern pike. Gametes were collected from spawning wild pike captured from a reference site outside of the oil sands area. Fertilized eggs were exposed to control water or increasing concentrations of tailings pond sediments for 21 days, coinciding with initiation of exogenous feeding and completion of yolk absorption. Developing Fish were examined for survival and changes in body weight, length, and development. Embryos exhibited increased developmental abnormalities and decreased growth and survival with increasing sediment concentration. Both sediment samples had similar levels of naphthenic acids and similar types of PAHs, with alkylated PAHs dominating. However, concentrations of total and alkylated PAHs differed between sediment samples and were related to increasing developmental abnormalities and decreased growth and survival. This is consistent with developmental changes observed with exposure to PAHs in other Fish species. These results provide information on the effects of tailings pond sediments comprising mixtures of PAHs and alkylated PAHs on the development and survival of a key species in the northern aquatic ecosystem.
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oil sands tailings pond sediment toxicity to early life stages of northern pike esox lucius
Science of The Total Environment, 2018Co-Authors: Jason C Raine, Leszek Romanowski, Daniel Turcotte, Joanne L ParrottAbstract:Abstract The Athabasca River in Alberta flows through natural sources of eroding oil sands bitumen and oil sands mining operations that may result in low level contamination of surface waters. Northern pike (Esox lucius) are apex predators and important food and Game Fish species native to the Athabasca River system. This species has the potential to be exposed to both natural and anthropogenic sources of contamination from oil sands related materials throughout its life cycle. Pike are difficult to rear in the laboratory and little information exists on the toxicity of oil sands related materials to this key indigenous Fish species. In this study, the potential effects of two sediment samples collected from different areas of one tailings pond in the Athabasca oil sands area are assessed in a daily renewal bioassay on early life stages of northern pike. Gametes were collected from spawning wild pike captured from a reference site outside of the oil sands area. Fertilized eggs were exposed to control water or increasing concentrations of tailings pond sediments for 21 days, coinciding with initiation of exogenous feeding and completion of yolk absorption. Developing Fish were examined for survival and changes in body weight, length, and development. Embryos exhibited increased developmental abnormalities and decreased growth and survival with increasing sediment concentration. Both sediment samples had similar levels of naphthenic acids and similar types of PAHs, with alkylated PAHs dominating. However, concentrations of total and alkylated PAHs differed between sediment samples and were related to increasing developmental abnormalities and decreased growth and survival. This is consistent with developmental changes observed with exposure to PAHs in other Fish species. These results provide information on the effects of tailings pond sediments comprising mixtures of PAHs and alkylated PAHs on the development and survival of a key species in the northern aquatic ecosystem.
Leszek Romanowski - One of the best experts on this subject based on the ideXlab platform.
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oil sands tailings pond sediment toxicity to early life stages of northern pike esox lucius
Science of The Total Environment, 2018Co-Authors: Jason C Raine, Leszek Romanowski, Daniel Turcotte, Joanne L ParrottAbstract:Abstract The Athabasca River in Alberta flows through natural sources of eroding oil sands bitumen and oil sands mining operations that may result in low level contamination of surface waters. Northern pike (Esox lucius) are apex predators and important food and Game Fish species native to the Athabasca River system. This species has the potential to be exposed to both natural and anthropogenic sources of contamination from oil sands related materials throughout its life cycle. Pike are difficult to rear in the laboratory and little information exists on the toxicity of oil sands related materials to this key indigenous Fish species. In this study, the potential effects of two sediment samples collected from different areas of one tailings pond in the Athabasca oil sands area are assessed in a daily renewal bioassay on early life stages of northern pike. Gametes were collected from spawning wild pike captured from a reference site outside of the oil sands area. Fertilized eggs were exposed to control water or increasing concentrations of tailings pond sediments for 21 days, coinciding with initiation of exogenous feeding and completion of yolk absorption. Developing Fish were examined for survival and changes in body weight, length, and development. Embryos exhibited increased developmental abnormalities and decreased growth and survival with increasing sediment concentration. Both sediment samples had similar levels of naphthenic acids and similar types of PAHs, with alkylated PAHs dominating. However, concentrations of total and alkylated PAHs differed between sediment samples and were related to increasing developmental abnormalities and decreased growth and survival. This is consistent with developmental changes observed with exposure to PAHs in other Fish species. These results provide information on the effects of tailings pond sediments comprising mixtures of PAHs and alkylated PAHs on the development and survival of a key species in the northern aquatic ecosystem.
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oil sands tailings pond sediment toxicity to early life stages of northern pike esox lucius
Science of The Total Environment, 2018Co-Authors: Jason C Raine, Leszek Romanowski, Daniel Turcotte, Joanne L ParrottAbstract:Abstract The Athabasca River in Alberta flows through natural sources of eroding oil sands bitumen and oil sands mining operations that may result in low level contamination of surface waters. Northern pike (Esox lucius) are apex predators and important food and Game Fish species native to the Athabasca River system. This species has the potential to be exposed to both natural and anthropogenic sources of contamination from oil sands related materials throughout its life cycle. Pike are difficult to rear in the laboratory and little information exists on the toxicity of oil sands related materials to this key indigenous Fish species. In this study, the potential effects of two sediment samples collected from different areas of one tailings pond in the Athabasca oil sands area are assessed in a daily renewal bioassay on early life stages of northern pike. Gametes were collected from spawning wild pike captured from a reference site outside of the oil sands area. Fertilized eggs were exposed to control water or increasing concentrations of tailings pond sediments for 21 days, coinciding with initiation of exogenous feeding and completion of yolk absorption. Developing Fish were examined for survival and changes in body weight, length, and development. Embryos exhibited increased developmental abnormalities and decreased growth and survival with increasing sediment concentration. Both sediment samples had similar levels of naphthenic acids and similar types of PAHs, with alkylated PAHs dominating. However, concentrations of total and alkylated PAHs differed between sediment samples and were related to increasing developmental abnormalities and decreased growth and survival. This is consistent with developmental changes observed with exposure to PAHs in other Fish species. These results provide information on the effects of tailings pond sediments comprising mixtures of PAHs and alkylated PAHs on the development and survival of a key species in the northern aquatic ecosystem.
Daniel Turcotte - One of the best experts on this subject based on the ideXlab platform.
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oil sands tailings pond sediment toxicity to early life stages of northern pike esox lucius
Science of The Total Environment, 2018Co-Authors: Jason C Raine, Leszek Romanowski, Daniel Turcotte, Joanne L ParrottAbstract:Abstract The Athabasca River in Alberta flows through natural sources of eroding oil sands bitumen and oil sands mining operations that may result in low level contamination of surface waters. Northern pike (Esox lucius) are apex predators and important food and Game Fish species native to the Athabasca River system. This species has the potential to be exposed to both natural and anthropogenic sources of contamination from oil sands related materials throughout its life cycle. Pike are difficult to rear in the laboratory and little information exists on the toxicity of oil sands related materials to this key indigenous Fish species. In this study, the potential effects of two sediment samples collected from different areas of one tailings pond in the Athabasca oil sands area are assessed in a daily renewal bioassay on early life stages of northern pike. Gametes were collected from spawning wild pike captured from a reference site outside of the oil sands area. Fertilized eggs were exposed to control water or increasing concentrations of tailings pond sediments for 21 days, coinciding with initiation of exogenous feeding and completion of yolk absorption. Developing Fish were examined for survival and changes in body weight, length, and development. Embryos exhibited increased developmental abnormalities and decreased growth and survival with increasing sediment concentration. Both sediment samples had similar levels of naphthenic acids and similar types of PAHs, with alkylated PAHs dominating. However, concentrations of total and alkylated PAHs differed between sediment samples and were related to increasing developmental abnormalities and decreased growth and survival. This is consistent with developmental changes observed with exposure to PAHs in other Fish species. These results provide information on the effects of tailings pond sediments comprising mixtures of PAHs and alkylated PAHs on the development and survival of a key species in the northern aquatic ecosystem.
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oil sands tailings pond sediment toxicity to early life stages of northern pike esox lucius
Science of The Total Environment, 2018Co-Authors: Jason C Raine, Leszek Romanowski, Daniel Turcotte, Joanne L ParrottAbstract:Abstract The Athabasca River in Alberta flows through natural sources of eroding oil sands bitumen and oil sands mining operations that may result in low level contamination of surface waters. Northern pike (Esox lucius) are apex predators and important food and Game Fish species native to the Athabasca River system. This species has the potential to be exposed to both natural and anthropogenic sources of contamination from oil sands related materials throughout its life cycle. Pike are difficult to rear in the laboratory and little information exists on the toxicity of oil sands related materials to this key indigenous Fish species. In this study, the potential effects of two sediment samples collected from different areas of one tailings pond in the Athabasca oil sands area are assessed in a daily renewal bioassay on early life stages of northern pike. Gametes were collected from spawning wild pike captured from a reference site outside of the oil sands area. Fertilized eggs were exposed to control water or increasing concentrations of tailings pond sediments for 21 days, coinciding with initiation of exogenous feeding and completion of yolk absorption. Developing Fish were examined for survival and changes in body weight, length, and development. Embryos exhibited increased developmental abnormalities and decreased growth and survival with increasing sediment concentration. Both sediment samples had similar levels of naphthenic acids and similar types of PAHs, with alkylated PAHs dominating. However, concentrations of total and alkylated PAHs differed between sediment samples and were related to increasing developmental abnormalities and decreased growth and survival. This is consistent with developmental changes observed with exposure to PAHs in other Fish species. These results provide information on the effects of tailings pond sediments comprising mixtures of PAHs and alkylated PAHs on the development and survival of a key species in the northern aquatic ecosystem.
Carol A Stepien - One of the best experts on this subject based on the ideXlab platform.
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accurate detection and quantification of the Fish viral hemorrhagic septicemia virus vhsv with a two color fluorometric real time pcr assay
PLOS ONE, 2013Co-Authors: Lindsey R Pierce, James C Willey, Vrushalee V Palsule, Jiyoun Yeo, Brian S Shepherd, Erin L Crawford, Carol A StepienAbstract:Viral Hemorrhagic Septicemia virus (VHSv) is one of the world’s most serious Fish pathogens, infecting .80 marine, freshwater, and estuarine Fish species from Eurasia and North America. A novel and especially virulent strain – IVb – appeared in the Great Lakes in 2003, has killed many Game Fish species in a series of outbreaks in subsequent years, and shut down interstate transport of baitFish. Cell culture is the diagnostic method approved by the USDA-APHIS, which takes a month or longer, lacks sensitivity, and does not quantify the amount of virus. We thus present a novel, easy, rapid, and highly sensitive real-time quantitative reverse transcription PCR (qRT-PCR) assay that incorporates synthetic competitive template internal standards for quality control to circumvent false negative results. Results demonstrate high signal-toanalyte response (slope=1.0060.02) and a linear dynamic range that spans seven orders of magnitude (R 2 =0.99), ranging from 6 to 6,000,000 molecules. Infected Fishes are found to harbor levels of virus that range to 1,200,000 VHSv molecules/ 10 6 actb1 molecules with 1,000 being a rough cut-off for clinical signs of disease. This new assay is rapid, inexpensive, and has significantly greater accuracy than other published qRT-PCR tests and traditional cell culture diagnostics.
