The Experts below are selected from a list of 7332 Experts worldwide ranked by ideXlab platform
Vincenzo Fogliano - One of the best experts on this subject based on the ideXlab platform.
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Garlic Extract prevents CCl4-induced liver fibrosis in rats: The role of tissue transglutaminase
Digestive and Liver Disease, 2009Co-Authors: Giuseppe D'argenio, D.c. Amoruso, Paola Vitaglione, Antonietta Romano, Maria T. Ribecco, Maria D'armiento, Filomena Morisco, Ernesto Mezza, Gloria Mazzone, Vincenzo FoglianoAbstract:BACKGROUND AND AIM: Tissue transglutaminase contributes to liver damage in the development of hepatic fibrosis. In a model of neurodegeneration, the therapeutic benefit of cystamine has been partly attributed to its inhibition of transglutaminase activity. Garlic Extract contains many compounds structurally related to cystamine. We investigated the anti-fibrotic effect of Garlic Extract and cystamine as specific tissue transglutaminase inhibitors. METHODS: Rat liver fibrosis was induced by intraperitoneal injection of carbon tetrachloride (CCl(4)) for 7 weeks. Cystamine or Garlic Extract was administrated by daily intraperitoneal injection, starting from the day after the first administration of CCl(4). Hepatic function, histology, tissue transglutaminase immunostaining and image analysis to quantify Red Sirius stained collagen deposition were examined. Reverse transcription-polymerase chain reaction to detect alpha-SMA, IL-1beta and tissue transglutaminase expression and Western blot for tissue transglutaminase protein amount were performed. Transglutaminase activity was assayed on liver homogenates by a radio-enzymatic method. RESULTS: Transglutaminase activity was increased in CCl(4) group and reduced by cystamine and Garlic Extract (p
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Garlic Extract prevents CCl4-induced liver fibrosis in rats: The role of tissue transglutaminase
Digestive and liver disease : official journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver, 2009Co-Authors: Giuseppe D'argenio, D.c. Amoruso, Paola Vitaglione, Antonietta Romano, Maria T. Ribecco, Maria D'armiento, Filomena Morisco, Ernesto Mezza, Gloria Mazzone, Vincenzo FoglianoAbstract:Background and aim: Tissue transglutaminase contributes to liver damage in the development of hepatic fibrosis. In a model of neurodegeneration, the therapeutic benefit of cystamine has been partly attributed to its inhibition of transglutaminase activity. Garlic Extract contains many compounds structurally related to cystamine. We investigated the anti-fibrotic effect of Garlic Extract and cystamine as specific tissue transglutaminase inhibitors. Methods: Rat liver fibrosis was induced by intraperitoneal injection of carbon tetrachloride (CCl4) for 7 weeks. Cystamine or Garlic Extract was administrated by daily intraperitoneal injection, starting from the day after the first administration of CCl4. Hepatic function, histology, tissue transglutaminase immunostaining and image analysis to quantify Red Sirius stained collagen deposition were examined. Reverse transcription-polymerase chain reaction to detect alpha-SMA, IL-1 and tissue transglutaminase expression and Western blot for tissue transglutaminase protein amount were performed. Transglutaminase activity was assayed on liver homogenates by a radio-enzymatic method. Results: Transglutaminase activity was increased in CCl4 group and reduced by cystamine and Garlic Extract (p < 0.05). Treatment with cystamine and Garlic Extract reduced the liver fibrosis and collagen deposition, particularly in the Garlic Extract group (p < 0.01). Moreover, the liver damage improved and serum alanine aminotransferase was decreased (p < 0.05). Tissue transglutaminase immunolocalised with collagen fibres and is mainly found in the ECM of damaged liver. Alpha-SMA, IL-1, tissue transglutaminase mRNA and tissue transglutaminase protein were down-regulated in the cystamine and Garlic Extract groups compared to controls. Conclusion: These findings concurrently suggest that transglutaminase may play a pivotal role in the pathogenesis of liver fibrosis and may identify Garlic cystamine-like molecules as a potential therapeutic strategy in the treatment of liver injury.
Azim Akbarzadeh - One of the best experts on this subject based on the ideXlab platform.
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Effects of Garlic Extract treatment in normal and streptozotocin diabetic rats infected with Candida albicans.
Indian Journal of Clinical Biochemistry, 2010Co-Authors: Mohammad Bokaeian, Alireza Nakhaee, Bita Moodi, Ali Farhangi, Azim AkbarzadehAbstract:The anti-candidial effect of Garlic Extract (Allium sativum L.) was investigated in normal and streptozotocin-induced diabetic rats. Diabetes was induced after a single intraperitoneal injection of streptozotocin (60 mg/kg). Rats were divided into six groups with fifteen rats in each group: (1) Normal control rats (2) Control rats + C. albicans (3) Control rats + Garlic Extract + C. albicans (4) Diabetic control rats (5) Diabetic rats + C. albicans (6) Diabetic rats + Garlic Extract + C. albicans. The concerned groups were inoculated with C.albicans on the 15 th day. At the end of one month experiment, fasted rats were killed by cervical decapitation. Blood was collected for estimation of glucose and C. albicans concentrations were estimated in liver and kidneys homogenates. A significant increase was observed in serum glucose levels in diabetic rats. A loss of bodyweight, polydipsia and polyphagia were observed in diabetic rats. Administration of alcoholic Extract of Garlic (0.25 g/kg body weight) reduced the hyperglycemia, polydipsia, polyphagia and associated weight loss of streptozotocin-treated rats. Administration of Garlic Extract significantly reduced C. albicans concentrations in liver and kidneys homogenates in infected control and diabetic rats. It is concluded that Garlic Extract improves candidia infection in diabetic rats.
Hyung Joo Suh - One of the best experts on this subject based on the ideXlab platform.
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physical stability antioxidative properties and photoprotective effects of a functionalized formulation containing black Garlic Extract
Journal of Photochemistry and Photobiology B-biology, 2012Co-Authors: Seon Hee Kim, Eun Young Jung, Duk Ho Kang, Un Jae Chang, Yang Hee Hong, Hyung Joo SuhAbstract:Abstract When Garlic is fermented, certain aspects of its bioactivity are changed. Black Garlic is a type of fermented Garlic used as a food ingredient in Asian cuisine. Black Garlic’s popularity has spread around the world as it has become a sought-after ingredient used in high-end cuisine. The formulations containing 10% black Garlic Extract or 10% normal Garlic Extract showed stable pH, color, precipitation, and organoleptic features, although these characteristics changed slightly. The 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging activities of the black Garlic formulation were significantly ( p p p
Eun Young Jung - One of the best experts on this subject based on the ideXlab platform.
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physical stability antioxidative properties and photoprotective effects of a functionalized formulation containing black Garlic Extract
Journal of Photochemistry and Photobiology B-biology, 2012Co-Authors: Seon Hee Kim, Eun Young Jung, Duk Ho Kang, Un Jae Chang, Yang Hee Hong, Hyung Joo SuhAbstract:Abstract When Garlic is fermented, certain aspects of its bioactivity are changed. Black Garlic is a type of fermented Garlic used as a food ingredient in Asian cuisine. Black Garlic’s popularity has spread around the world as it has become a sought-after ingredient used in high-end cuisine. The formulations containing 10% black Garlic Extract or 10% normal Garlic Extract showed stable pH, color, precipitation, and organoleptic features, although these characteristics changed slightly. The 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging activities of the black Garlic formulation were significantly ( p p p
Giuseppe D'argenio - One of the best experts on this subject based on the ideXlab platform.
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Garlic Extract prevents CCl4-induced liver fibrosis in rats: The role of tissue transglutaminase
Digestive and Liver Disease, 2009Co-Authors: Giuseppe D'argenio, D.c. Amoruso, Paola Vitaglione, Antonietta Romano, Maria T. Ribecco, Maria D'armiento, Filomena Morisco, Ernesto Mezza, Gloria Mazzone, Vincenzo FoglianoAbstract:BACKGROUND AND AIM: Tissue transglutaminase contributes to liver damage in the development of hepatic fibrosis. In a model of neurodegeneration, the therapeutic benefit of cystamine has been partly attributed to its inhibition of transglutaminase activity. Garlic Extract contains many compounds structurally related to cystamine. We investigated the anti-fibrotic effect of Garlic Extract and cystamine as specific tissue transglutaminase inhibitors. METHODS: Rat liver fibrosis was induced by intraperitoneal injection of carbon tetrachloride (CCl(4)) for 7 weeks. Cystamine or Garlic Extract was administrated by daily intraperitoneal injection, starting from the day after the first administration of CCl(4). Hepatic function, histology, tissue transglutaminase immunostaining and image analysis to quantify Red Sirius stained collagen deposition were examined. Reverse transcription-polymerase chain reaction to detect alpha-SMA, IL-1beta and tissue transglutaminase expression and Western blot for tissue transglutaminase protein amount were performed. Transglutaminase activity was assayed on liver homogenates by a radio-enzymatic method. RESULTS: Transglutaminase activity was increased in CCl(4) group and reduced by cystamine and Garlic Extract (p
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Garlic Extract prevents CCl4-induced liver fibrosis in rats: The role of tissue transglutaminase
Digestive and liver disease : official journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver, 2009Co-Authors: Giuseppe D'argenio, D.c. Amoruso, Paola Vitaglione, Antonietta Romano, Maria T. Ribecco, Maria D'armiento, Filomena Morisco, Ernesto Mezza, Gloria Mazzone, Vincenzo FoglianoAbstract:Background and aim: Tissue transglutaminase contributes to liver damage in the development of hepatic fibrosis. In a model of neurodegeneration, the therapeutic benefit of cystamine has been partly attributed to its inhibition of transglutaminase activity. Garlic Extract contains many compounds structurally related to cystamine. We investigated the anti-fibrotic effect of Garlic Extract and cystamine as specific tissue transglutaminase inhibitors. Methods: Rat liver fibrosis was induced by intraperitoneal injection of carbon tetrachloride (CCl4) for 7 weeks. Cystamine or Garlic Extract was administrated by daily intraperitoneal injection, starting from the day after the first administration of CCl4. Hepatic function, histology, tissue transglutaminase immunostaining and image analysis to quantify Red Sirius stained collagen deposition were examined. Reverse transcription-polymerase chain reaction to detect alpha-SMA, IL-1 and tissue transglutaminase expression and Western blot for tissue transglutaminase protein amount were performed. Transglutaminase activity was assayed on liver homogenates by a radio-enzymatic method. Results: Transglutaminase activity was increased in CCl4 group and reduced by cystamine and Garlic Extract (p < 0.05). Treatment with cystamine and Garlic Extract reduced the liver fibrosis and collagen deposition, particularly in the Garlic Extract group (p < 0.01). Moreover, the liver damage improved and serum alanine aminotransferase was decreased (p < 0.05). Tissue transglutaminase immunolocalised with collagen fibres and is mainly found in the ECM of damaged liver. Alpha-SMA, IL-1, tissue transglutaminase mRNA and tissue transglutaminase protein were down-regulated in the cystamine and Garlic Extract groups compared to controls. Conclusion: These findings concurrently suggest that transglutaminase may play a pivotal role in the pathogenesis of liver fibrosis and may identify Garlic cystamine-like molecules as a potential therapeutic strategy in the treatment of liver injury.
