The Experts below are selected from a list of 291 Experts worldwide ranked by ideXlab platform
Hiroyuki Okada - One of the best experts on this subject based on the ideXlab platform.
-
technique for single step lymphocyte isolation from an endoscopic biopsy specimen for the diagnosis of Gastrointestinal Lymphoma
MethodsX, 2020Co-Authors: Masaya Iwamuro, Takahide Takahashi, Natsuki Watanabe, Sizuma Omote, Katsunori Matsueda, Takehiro Tanaka, Daisuke Ennishi, Fumio Otsuka, Tadashi Yoshino, Hiroyuki OkadaAbstract:ABSTRACT In this paper, we introduce a simplified, one-step procedure for lymphocyte isolation from an endoscopically biopsied fragment. For lymphocyte isolation, an endoscopically harvested specimen and 5 mL of normal saline solution were placed in a wire mesh strainer set in a porcelain bowl. To obtain the lymphocyte suspension, the solid specimen was crushed using the rubber portion of a plunger of a 10 mL injection syringe. Flow cytometry was performed using the lymphocyte suspension. For validating our methods, the one-step lymphocyte isolation technique was used to perform flow cytometry on samples from 23 patients with (n = 12) or without (n = 11) Gastrointestinal Lymphoma. Flow cytometry of light chain expression was performed in all patient samples (feasibility: 100%). Sensitivity was 83.3% (10/12) and specificity was 100% (11/11). In conclusion, lymphocytes isolated from a single endoscopic biopsy specimen using our simplified and quick procedure are suitable for flow cytometry. Considering that flow cytometry has an important advantage of providing the results on the examination day itself, the results of this study suggest that flow cytometric analysis using our single-step lymphocyte isolation technique can be potentially used to diagnose Lymphoma in the Gastrointestinal mucosa. Bullet points We introduce a simplified, one-step procedure for lymphocyte isolation from an endoscopically biopsied fragment. Our technique is feasible for flow cytometric analysis in patients with Gastrointestinal Lymphoma as well as those with Gastrointestinal lesions that are suspected to be Lymphoma.
-
an endoscopic biopsy specimen contains adequate lymphocytes for flow cytometric analysis of light chain expression in the Gastrointestinal mucosa
Annals of Clinical and Laboratory Science, 2020Co-Authors: Masaya Iwamuro, Takahide Takahashi, Sizuma Omote, Katsunori Matsueda, Takehiro Tanaka, Daisuke Ennishi, Fumio Otsuka, Tadashi Yoshino, Hiroyuki OkadaAbstract:OBJECTIVE Flow cytometry has not been widely used in routine clinical practice for the diagnosis of Gastrointestinal Lymphoma; this is mainly because of the absence of an appropriate protocol. Here, we established a protocol for flow cytometric analysis of a single biopsy specimen from the Gastrointestinal mucosa and investigated its sensitivity and specificity. DESIGN In this prospective study, we enrolled patients with previously diagnosed Gastrointestinal Lymphoma and patients with Gastrointestinal lesions that were suspected to be Lymphoma. RESULTS Overall, 15 patients with gastric extranodal marginal zone Lymphoma of mucosa-associated lymphoid tissue (N=8), duodenal follicular Lymphoma (grade 1; N=5), and benign lymphoid hyperplasia (ileum, N=1, and rectum, N=1) were included in this study. Of these, lymphocytes were isolated from 14 patients (93.3%). There were 200,000-1,500,000 viable cells per patient. Biopsy specimens from 10 out of the 12 patients with Lymphoma were positive for light chain restriction; the two patients with benign lymphoid hyperplasia showed negative results. CONCLUSIONS An adequate number of lymphocytes for flow cytometry could be isolated from a single specimen of endoscopic mucosal biopsy from 93.3% of the patients. Overall, the sensitivity of flow cytometric analysis of light chain expression for the diagnosis of B-cell Lymphoma was 83.3%, and the specificity was 100%. Although further investigation is required as the sample size of the present study was small, our study suggests a potential option for diagnosing B-cell Lymphoma in the Gastrointestinal mucosa.
Leonidas G. Koniaris - One of the best experts on this subject based on the ideXlab platform.
-
Surgery does not adversely affect survival in primary Gastrointestinal Lymphoma.
Journal of surgical oncology, 2009Co-Authors: Michael C. Cheung, Nadine Housri, Michael P. Ogilvie, Juan E. Sola, Leonidas G. KoniarisAbstract:Objective To evaluate the impact of surgery on Gastrointestinal Lymphoma. Methods The Surveillance, Epidemiology, and End Results (SEER) database was queried from 1973 to 2005. Results A total of 17,222 cases of PGIL were identified. The overall incidence of PGIL was approximately 1.505 cases per 100,000. A significantly increasing incidence for PGIL was observed (APC = +4.67, P
-
surgery does not adversely affect survival in primary Gastrointestinal Lymphoma
Journal of Surgical Oncology, 2009Co-Authors: Michael C. Cheung, Nadine Housri, Michael P. Ogilvie, Juan E. Sola, Leonidas G. KoniarisAbstract:Objective To evaluate the impact of surgery on Gastrointestinal Lymphoma. Methods The Surveillance, Epidemiology, and End Results (SEER) database was queried from 1973 to 2005. Results A total of 17,222 cases of PGIL were identified. The overall incidence of PGIL was approximately 1.505 cases per 100,000. A significantly increasing incidence for PGIL was observed (APC = +4.67, P < 0.05). In the cases for which treatment data was available, resection occurred in roughly half of the patients. In univariate analysis, surgical extirpation did not improve survival (47 months vs. 76 months, P < 0.001), while radiation treatment improved median survival (77 months vs. 59 months, P < 0.001). Multivariate analysis revealed increasing age and male gender as independent predictors of decreased overall survival. Tumor location also was a significant predictor of outcome. Large B-cell Lymphoma type PGIL had a poorer prognosis than marginal zone B-cell Lymphoma. By multivariate analysis, surgery was not found to increase the risk of death (HR = 0.99). Conclusions No associated survival benefit for surgery in the treatment in Gastrointestinal Lymphoma was observed. Determination of Lymphoma should preclude surgical resection. Nonetheless, inadvertent extirpative surgery or in association with perforation does not appear to increase mortality. J. Surg. Oncol. 2009;100:59–64. © 2009 Wiley-Liss, Inc.
-
management of Gastrointestinal Lymphoma
Journal of The American College of Surgeons, 2003Co-Authors: Leonidas G. Koniaris, George T Drugas, Philip J Katzman, Rabih M SalloumAbstract:Despite numerous effective chemotherapeutic regimens developed to treat non-Hodgkin’s Lymphoma (NHL), it remains the sixth most common cause of cancerassociated deaths in the United States. Estimates from the American Cancer Society project that about 53,000 new cases of NHL will be diagnosed in the United States in 2002 and approximately half of those afflicted will die of their disease. The incidence of NHL has increased greatly since the early 1980s, in large part from increased HIV disease, though ubiquitous environmental and toxic exposures have also been implicated. NHL is more common in men (M/F ratio 1.5/1). The incidence of NHL in younger white men (ages 18 to 45) has stabilized since 1997 but might be increasing in both women and African Americans. Extranodal Lymphoma, or Lymphoma arising within solid organs, occurs in up to 40% of all cases. The Gastrointestinal (GI) tract is the most frequently involved extranodal site, accounting for up to half of all extranodal cases. Lymphomas can involve any part of the GI tract from oral cavity to rectum. Surgical resection can play an important role in the diagnosis and treatment of NHL involving the GI tract. The surgeon should maintain a high index of suspicion for GI Lymphomas and appreciate the critical role surgery might play in obtaining an accurate diagnosis and successfully managing these malignancies. This article reviews GI Lymphomas with particular emphasis on the role of surgery as part of a multidisciplinary approach to the diagnosis and management of NHL.
I. A. Al-mofleh - One of the best experts on this subject based on the ideXlab platform.
-
Endoscopic features of primary upper Gastrointestinal Lymphoma.
Journal of clinical gastroenterology, 1994Co-Authors: I. A. Al-moflehAbstract:Primary upper Gastrointestinal Lymphoma (PUGIL) is relatively common in the Middle East, and upper Gastrointestinal (UGI) endoscopy is very useful for its diagnosis. Analysis of 33 patients with PUGIL revealed that ulceration, mainly superficial and/or multicentric, in 24 (73%) patients was the most
-
Complications of Primary Upper Gastrointestinal Lymphoma
Annals of Saudi medicine, 1992Co-Authors: I. A. Al-moflehAbstract:ABSTRACT This study reports the Gastrointestinal complications of primary upper Gastrointestinal Lymphoma (PUGIL). Of thirty-three patients with PUGIL, twenty-one (64%) had complications. These inc...
Damon Choy - One of the best experts on this subject based on the ideXlab platform.
-
Prognostic factors for primary Gastrointestinal Lymphoma
Hematological oncology, 1995Co-Authors: Raymond Liang, David Todd, T. K. Chan, Edmond Chiu, Albert K. W. Lie, Yok-lam Kwong, Damon ChoyAbstract:The Gastrointestinal tract is a common primary extranodal site for non-Hodgkin's Lymphoma. There is however no uniform consensus on its pathological classification, clinical staging system and management. This paper reports the experience in the management of 425 Chinese patients with primary Gastrointestinal Lymphoma in Hong Kong from January 1975 to June 1993. There were 230 (54 per cent) males and 195 (46 per cent) females. Their median age was 53 years. The primary sites were: the esophagus in three (1 per cent), stomach in 238 (56 per cent), small intestine in 131 (31 per cent) and large intestine in 53 (12 per cent). According to the Working Formulation, there were 20 (4.7 per cent) small lymphocytic, 10 (2.4 per cent) follicular small cleaved cell, 15 (3.5 per cent) follicular mixed, five (1.2 per cent) follicular large cell, 40 (9.4 per cent) diffuse small cleaved cell, 50 (12 per cent) diffuse mixed, 181 (43 per cent) diffuse large cell, 30 (7.1 per cent) immunoblastic, five (1.2 per cent) lymphoblastic, 10 (2.4 per cent) diffuse small non-cleaved cell and 50 (14 per cent) unclassifiable Lymphoma. Immunophenotyping was performed in 199 (47 per cent) patients: 90 per cent B-cell, 7 per cent T-cell and 3 per cent uncertain. According to a Manchester system, 81 (19 per cent) patients had stage I disease, 44 (10 per cent) stage II, 85 (20 per cent) stage III and 215 (51 per cent) stage IV. B symptoms were present in 275 (65 per cent) patients and bulky disease in 104 (25 per cent). Surgery followed by chemotherapy was the mainstay of treatment. Of the 408 patients treated, 63 per cent had a complete remission with relapse rate of 42 per cent. For those with complete remission, 47 per cent were free from disease at 5 years. The overall median survival of all patients was 45 per cent at 5 years. Multivariate analysis revealed that significant independent prognostic factors predicting better survival were young age of < 60 years, low grade histology, stage I and II disease and absence of bulky tumour. For gastric Lymphoma, aggressive surgery did not significantly improve their outcome. Chemotherapy appears to play an important role in the management of Gastrointestinal Lymphoma. Better classification of the primary Gastrointestinal Lymphoma and more refined stratification of the patients according to the prognostic variables may allow individualization of treatment. Prospective randomized studies are essential to define the relative roles of surgery, chemotherapy and radiotherapy.
Masaya Iwamuro - One of the best experts on this subject based on the ideXlab platform.
-
technique for single step lymphocyte isolation from an endoscopic biopsy specimen for the diagnosis of Gastrointestinal Lymphoma
MethodsX, 2020Co-Authors: Masaya Iwamuro, Takahide Takahashi, Natsuki Watanabe, Sizuma Omote, Katsunori Matsueda, Takehiro Tanaka, Daisuke Ennishi, Fumio Otsuka, Tadashi Yoshino, Hiroyuki OkadaAbstract:ABSTRACT In this paper, we introduce a simplified, one-step procedure for lymphocyte isolation from an endoscopically biopsied fragment. For lymphocyte isolation, an endoscopically harvested specimen and 5 mL of normal saline solution were placed in a wire mesh strainer set in a porcelain bowl. To obtain the lymphocyte suspension, the solid specimen was crushed using the rubber portion of a plunger of a 10 mL injection syringe. Flow cytometry was performed using the lymphocyte suspension. For validating our methods, the one-step lymphocyte isolation technique was used to perform flow cytometry on samples from 23 patients with (n = 12) or without (n = 11) Gastrointestinal Lymphoma. Flow cytometry of light chain expression was performed in all patient samples (feasibility: 100%). Sensitivity was 83.3% (10/12) and specificity was 100% (11/11). In conclusion, lymphocytes isolated from a single endoscopic biopsy specimen using our simplified and quick procedure are suitable for flow cytometry. Considering that flow cytometry has an important advantage of providing the results on the examination day itself, the results of this study suggest that flow cytometric analysis using our single-step lymphocyte isolation technique can be potentially used to diagnose Lymphoma in the Gastrointestinal mucosa. Bullet points We introduce a simplified, one-step procedure for lymphocyte isolation from an endoscopically biopsied fragment. Our technique is feasible for flow cytometric analysis in patients with Gastrointestinal Lymphoma as well as those with Gastrointestinal lesions that are suspected to be Lymphoma.
-
an endoscopic biopsy specimen contains adequate lymphocytes for flow cytometric analysis of light chain expression in the Gastrointestinal mucosa
Annals of Clinical and Laboratory Science, 2020Co-Authors: Masaya Iwamuro, Takahide Takahashi, Sizuma Omote, Katsunori Matsueda, Takehiro Tanaka, Daisuke Ennishi, Fumio Otsuka, Tadashi Yoshino, Hiroyuki OkadaAbstract:OBJECTIVE Flow cytometry has not been widely used in routine clinical practice for the diagnosis of Gastrointestinal Lymphoma; this is mainly because of the absence of an appropriate protocol. Here, we established a protocol for flow cytometric analysis of a single biopsy specimen from the Gastrointestinal mucosa and investigated its sensitivity and specificity. DESIGN In this prospective study, we enrolled patients with previously diagnosed Gastrointestinal Lymphoma and patients with Gastrointestinal lesions that were suspected to be Lymphoma. RESULTS Overall, 15 patients with gastric extranodal marginal zone Lymphoma of mucosa-associated lymphoid tissue (N=8), duodenal follicular Lymphoma (grade 1; N=5), and benign lymphoid hyperplasia (ileum, N=1, and rectum, N=1) were included in this study. Of these, lymphocytes were isolated from 14 patients (93.3%). There were 200,000-1,500,000 viable cells per patient. Biopsy specimens from 10 out of the 12 patients with Lymphoma were positive for light chain restriction; the two patients with benign lymphoid hyperplasia showed negative results. CONCLUSIONS An adequate number of lymphocytes for flow cytometry could be isolated from a single specimen of endoscopic mucosal biopsy from 93.3% of the patients. Overall, the sensitivity of flow cytometric analysis of light chain expression for the diagnosis of B-cell Lymphoma was 83.3%, and the specificity was 100%. Although further investigation is required as the sample size of the present study was small, our study suggests a potential option for diagnosing B-cell Lymphoma in the Gastrointestinal mucosa.
