Gastrointestinal System

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Ali Mobasheri - One of the best experts on this subject based on the ideXlab platform.

Tonghui Ma - One of the best experts on this subject based on the ideXlab platform.

  • Cloning and characterization of porcine aquaporin 1 water channel expressed extensively in Gastrointestinal System
    World Journal of Gastroenterology, 2006
    Co-Authors: Li-na Xu, Yong Jiang, Ying Wang, Baoxue Yang, Hong Yang, Tonghui Ma
    Abstract:

    AIM: To clone and characterize the porcine aquaporins (AQPs) in the Gastrointestinal System. METHODS: A PCR-based cloning strategy and RACE were used to clone full-length AQP coding sequence from reversely transcribed pig liver cDNA. Stopped-flow light scattering and a YFP-based fluorescence method were used to measure the osmotic water permeability of erythrocytes and the stably transfected CHO cells. RT-PCR, Northern blot, and immunohistochemistry were used to determine the Gastrointestinal expression and localization of cloned AQPs. Protein expression in transfected cells and red blood cells was analyzed by Western blot. RESULTS: An 813 bp cDNA encoding a 271 amino acid porcine aquaporin (designated pAQP1) was cloned from liver mRNA (pAQP1 has a 93% identity with human AQP1 and contains two NPA motifs conserved in AQP family, one consensus sequence for N-linked glycosylation, and one mercury-sensitive site at cysteine 191). RT-PCR analysis revealed extensive expression of pAQP1 mRNA in porcine digestive glands and gut. Northern blot showed a single 3.0 kb transcript in selected digestive organs. pAQP1 protein was localized at central lacteals of the small intestine, microvessles of salivary glands, as well as epithelium of intrahepatic bile ducts by immunoperoxydase. High osmotic water permeability that is inhibitable by HgCl2 was detected in porcine erythrocytes and CHO cells stably transfected with pAQP1 cDNA. Immunoblot analysis of porcine erythrocytes and pAQP-transfected CHO cells revealed an unglycosylated 28 ku band and larger glycosylated proteins. CONCLUSION: pAQP1 is the first porcine aquaporin that can be molecularly identified so far. The broad distribution of pAQP1 in epithelium and endothelium of porcine digestive organs may suggest an important role of channel-mediated water transport in fluid secretion/absorption as well as in digestive function and pathophysiology of the Gastrointestinal System.

Nevin A Öztürk - One of the best experts on this subject based on the ideXlab platform.

  • Üst Gastrointestinal Sistem Kanamali Hastalarin Epidemiyolojisi ve Endoskopik Bulgulari Epidemiology and Endoscopic Findings of the Patients Suffering from Upper Gastrointestinal System Bleeding
    2020
    Co-Authors: Banu Kara, Nevin A Öztürk
    Abstract:

    Objective: In the present study, we aim to investigate general and endoscopic findings of the patients who were hospitalized in our clinic because of upper Gastrointestinal System bleeding (UGSB). Methods: The files of 403 patients who applied to our clinic between January 2014 and December 2014 with UGSB diagnosis were scanned retrospectively. The demographic, laboratorial and endoscopic findings of the patients were examined. Results: The average age of 403 patients were 61.12±17.1 (min. 17- max. 96) and while 263 of these patients were male (65.3(%), 140 of them were female (34.7%). Of all, 234 patients had an additional disease. The most frequently observed diseases were hypertension, diabetes mellitus and coronary artery. 259 (64.3%) of the patients used to take at least one drug and 212 (52.6%) of the patients used to get non-steroid anti-inflammatory drugs and/or aspirin. The most common reasons of UGSB were duodenal ulcer in 158 patients (39.2%), stomach ulcer in 97 patients (24%), erosive gastroduodenitis in 66 patients (16.3%) and esophageal varices in 38 patients (9.4%). Unfortunately, 18 of the patients died. Conclusion: The most common reason of UGSB is duodenal ulcer bleeding. In spite of the technological development nowadays, it is a disease which has mortality.

  • Epidemiology and Endoscopic Findings of the Patients Suffering from Upper Gastrointestinal System Bleeding
    Dicle Medical Journal Dicle Tip Dergisi, 2016
    Co-Authors: Mehmet Suat Yalçın, Banu Kara, Nevin A Öztürk, Şehmus Ölmez, Burcak Evren Tasdogan
    Abstract:

    Objective: In the present study, we aim to investigate general and endoscopic findings of the patients who were hospitalized in our clinic because of upper Gastrointestinal System bleeding (UGSB). Methods: The files of 403 patients who applied to our clinic between January 2014 and December 2014 with UGSB diagnosis were scanned retrospectively. The demographic, laboratorial and endoscopic findings of the patients were examined. Results: The average age of 403 patients were 61.12±17.1 (min. 17- max. 96) and while 263 of these patients were male (65.3(%), 140 of them were female (34.7%). Of all, 234 patients had an additional disease. The most frequently observed diseases were hypertension, diabetes mellitus and coronary artery. 259 (64.3%) of the patients used to take at least one drug and 212 (52.6%) of the patients used to get non-steroid anti-inflammatory drugs and/or aspirin. The most common reasons of UGSB were duodenal ulcer in 158 patients (39.2%), stomach ulcer in 97 patients (24%), erosive gastroduodenitis in 66 patients (16.3%) and esophageal varices in 38 patients (9.4%). Unfortunately, 18 of the patients died. Conclusion: The most common reason of UGSB is duodenal ulcer bleeding. In spite of the technological development nowadays, it is a disease which has mortality. Key words: Upper Gastrointestinal System bleeding, duodenal ulcer, gastric ulcer, variceal hemorrhage

Tevfik Sabuncu - One of the best experts on this subject based on the ideXlab platform.

  • The effect of upper Gastrointestinal System endoscopy process on serum oxidative stress levels.
    Wiener Klinische Wochenschrift, 2016
    Co-Authors: Mehmet Nuri Turan, Mehmet Aslan, Filiz F. Bolukbas, Cengiz Bolukbas, Sahbettin Selek, Tevfik Sabuncu
    Abstract:

    Some authors have investigated the effects of oxidative stress in some process such as undergoing laparoscopic. However, the effect of upper Gastrointestinal System endoscopy process on oxidative stress is unclear. We evaluated the short-term effect of upper Gastrointestinal System endoscopy process on oxidative stress. Thirty patients who underwent endoscopy process and 20 healthy controls were enrolled in the prospective study. Serum total antioxidant capacity and total oxidant status measurements were measured before and after endoscopy process. The ratio percentage of total oxidant status to total antioxidant capacity was regarded as oxidative stress index. Before endoscopy process, serum total antioxidant capacity levels were higher, while serum total oxidant status levels and oxidative stress index values were lower in patients than controls, but this difference was not statistically significant (all, p > 0.05). After endoscopy process, serum total antioxidant capacity and total oxidant status levels were significantly higher in patients than before endoscopy process (both, p 0.05). We observed that serum TAC and TOS levels were increased in patients who underwent endoscopy process after endoscopy process. However, short-time upper Gastrointestinal System endoscopy process did not cause an important change in the oxidative stress index. Further studies enrolling a larger number of patients are required to clarify the results obtained here.

Laura W. Lamps - One of the best experts on this subject based on the ideXlab platform.

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