The Experts below are selected from a list of 276 Experts worldwide ranked by ideXlab platform
Christine Carapito - One of the best experts on this subject based on the ideXlab platform.
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Tube-Gel: A Fast and Effective Sample Preparation Method for High-Throughput Quantitative Proteomics
2019Co-Authors: Leslie Muller, Luc Fornecker, Sarah Cianférani, Christine CarapitoAbstract:Sample preparation is a key step in proteomics workflows. Tube-Gel (TG) is a fast and repeatable Sample preparation method that consists in the instantaneous trapping of the Sample in a polyacrylamide Gel matrix. It takes advantage of in-Gel Sample preparations by allowing the use of high concentrations of sodium-dodecyl sulfate but avoids the time-consuming step of electrophoresis. Therefore, TG limits the Sample handling and is thus particularly suitable for high-throughput quantitative proteomics when large Sample numbers have to be processed, as it is often the case in biomarker research and clinical proteomics projects.
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extended investigation of tube Gel Sample preparation a versatile and simple choice for high throughput quantitative proteomics
Scientific Reports, 2018Co-Authors: Leslie Muller, Luc Fornecker, Sarah Cianférani, Marie Chion, Alain Van Dorsselaer, Thierry Rabilloud, Christine CarapitoAbstract:Sample preparation for quantitative proteomics is a crucial step to ensure the repeatability and the accuracy of the results. However, there is no universal method compatible with the wide variety of protein extraction buffers currently used. We have recently demonstrated the compatibility of tube-Gel with SDS-based buffers and its efficiency for label-free quantitative proteomics by comparing it to stacking Gel and liquid digestion. Here, we investigated the compatibility of tube-Gel with alternatives to SDS-based buffers allowing notably the extraction of proteins in various pH conditions. We also explored the use of photopolymerization to extend the number of possibilities, as it is compatible with a wide range of pH and is non-oxidative. To achieve this goal, we compared six extraction buffers in combination with two polymerization conditions to further optimize the tube-Gel protocol and evaluate its versatility. Identification and quantitative results demonstrated the compatibility of tube-Gel with all tested conditions by overall raising quite comparable results. In conclusion, tube-Gel is a versatile and simple Sample preparation method for large-scale quantitative proteomics applications. Complete datasets are available via ProteomeXchange with identifier PXD008656.
Leslie Muller - One of the best experts on this subject based on the ideXlab platform.
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Tube-Gel: A Fast and Effective Sample Preparation Method for High-Throughput Quantitative Proteomics
2019Co-Authors: Leslie Muller, Luc Fornecker, Sarah Cianférani, Christine CarapitoAbstract:Sample preparation is a key step in proteomics workflows. Tube-Gel (TG) is a fast and repeatable Sample preparation method that consists in the instantaneous trapping of the Sample in a polyacrylamide Gel matrix. It takes advantage of in-Gel Sample preparations by allowing the use of high concentrations of sodium-dodecyl sulfate but avoids the time-consuming step of electrophoresis. Therefore, TG limits the Sample handling and is thus particularly suitable for high-throughput quantitative proteomics when large Sample numbers have to be processed, as it is often the case in biomarker research and clinical proteomics projects.
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extended investigation of tube Gel Sample preparation a versatile and simple choice for high throughput quantitative proteomics
Scientific Reports, 2018Co-Authors: Leslie Muller, Luc Fornecker, Sarah Cianférani, Marie Chion, Alain Van Dorsselaer, Thierry Rabilloud, Christine CarapitoAbstract:Sample preparation for quantitative proteomics is a crucial step to ensure the repeatability and the accuracy of the results. However, there is no universal method compatible with the wide variety of protein extraction buffers currently used. We have recently demonstrated the compatibility of tube-Gel with SDS-based buffers and its efficiency for label-free quantitative proteomics by comparing it to stacking Gel and liquid digestion. Here, we investigated the compatibility of tube-Gel with alternatives to SDS-based buffers allowing notably the extraction of proteins in various pH conditions. We also explored the use of photopolymerization to extend the number of possibilities, as it is compatible with a wide range of pH and is non-oxidative. To achieve this goal, we compared six extraction buffers in combination with two polymerization conditions to further optimize the tube-Gel protocol and evaluate its versatility. Identification and quantitative results demonstrated the compatibility of tube-Gel with all tested conditions by overall raising quite comparable results. In conclusion, tube-Gel is a versatile and simple Sample preparation method for large-scale quantitative proteomics applications. Complete datasets are available via ProteomeXchange with identifier PXD008656.
Tung Sheng Shih - One of the best experts on this subject based on the ideXlab platform.
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Application of microwave-assisted desorption/headspace solid-phase microextraction as pretreatment step in the gas chromatographic determination of 1-naphthylamine in silica Gel adsorbent.
Talanta, 2006Co-Authors: Tung Sheng ShihAbstract:Abstract Pretreatment of silica Gel Sample containing 1-naphthylamine by microwave-assisted desorption (MAD) coupled to in situ headspace solid phase microextraction (HS-SPME) has been investigated as a possible alternative to conventional methods prior to gas chromatographic (GC) analysis. The 1-naphthylamine desorbs from silica Gel to headspace under microwave irradiation, and directly absorbs onto a SPME fiber located in a controlled-temperature headspace area. After being collected on the SPME fiber, and desorbed in the GC injection port, 1-naphthylamine is analyzed by GC-FID. Parameters that influence the extraction efficiency of the MAD/HS-SPME, such as the extraction media and its pH, the microwave irradiation power and irradiation time as well as desorption conditions of the GC injector, have been investigated. Experimental results indicate that the extraction of a 150 mg silica Gel Sample by using 0.8 ml of 1.0 M NaOH solution and a PDMS/DVB fiber under high-powered irradiation (477 W) for 5 min maximizes the extraction efficiency. Desorption of 1-naphthylamine from the SPME fiber in GC injector is optimal at 250 °C held for 3 min. The detection limit of method is 8.30 ng. The detected quantity of 1-naphthylamine obtained by the proposed method is 33.3 times of that obtained by the conventional solvent extraction method for the silica Gel Sample containing 100 ng of 1-naphthylamine. It provides a simple, fast, sensitive and organic-solvent-free pretreatment procedure prior to the analysis of 1-naphthylamine collected on a silica Gel adsorbent.
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Application of microwave-assisted desorption/headspace solid-phase microextraction as pretreatment step in the gas chromatographic determination of 1-naphthylamine in silica Gel adsorbent.
Talanta, 2006Co-Authors: Cheing-tong Yan, Jen-fon Jen, Tung Sheng ShihAbstract:Pretreatment of silica Gel Sample containing 1-naphthylamine by microwave-assisted desorption (MAD) coupled to in situ headspace solid phase microextraction (HS-SPME) has been investigated as a possible alternative to conventional methods prior to gas chromatographic (GC) analysis. The 1-naphthylamine desorbs from silica Gel to headspace under microwave irradiation, and directly absorbs onto a SPME fiber located in a controlled-temperature headspace area. After being collected on the SPME fiber, and desorbed in the GC injection port, 1-naphthylamine is analyzed by GC-FID. Parameters that influence the extraction efficiency of the MAD/HS-SPME, such as the extraction media and its pH, the microwave irradiation power and irradiation time as well as desorption conditions of the GC injector, have been investigated. Experimental results indicate that the extraction of a 150mg silica Gel Sample by using 0.8ml of 1.0M NaOH solution and a PDMS/DVB fiber under high-powered irradiation (477W) for 5min maximizes the extraction efficiency. Desorption of 1-naphthylamine from the SPME fiber in GC injector is optimal at 250 degrees C held for 3min. The detection limit of method is 8.30ng. The detected quantity of 1-naphthylamine obtained by the proposed method is 33.3 times of that obtained by the conventional solvent extraction method for the silica Gel Sample containing 100ng of 1-naphthylamine. It provides a simple, fast, sensitive and organic-solvent-free pretreatment procedure prior to the analysis of 1-naphthylamine collected on a silica Gel adsorbent.
Changsuk Kong - One of the best experts on this subject based on the ideXlab platform.
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effects of lubrication and Sample dimensions on compression property of fish meat Gels
Food Research International, 2005Co-Authors: Changsuk KongAbstract:Abstract Effects of lubrication and Sample dimension on compression property of fish–meat Gel were investigated using the modified Mooney–Rivlin equation. Cylindrical fish–meat Gels were cut into 26 mm in diameter and 10, 20 and 30 mm in heights, respectively. Lubrication of Samples led to the lower stress–strain curve. Stress at fracture was dependent on Sample dimension in non-lubricated test. In lubricated compression, the dependant was reduced and the agreement of stress–strain data for all Samples was observed at the low strain region. The similar results were observed in comparing compression properties of non-lubricated and lubricated fish–meat Gels according to the modified Mooney–Rivlin equation. In order to reduce friction problems in compression test of fish–meat Gels, the experiment setting can be suggested as follows: for the Samples with small dimensions, the surface of Gel Sample has to be lubricated, or Gel Samples have to be made with large dimensions.
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Effects of lubrication and Sample dimensions on compression property of fish–meat Gels
Food Research International, 2005Co-Authors: Changsuk KongAbstract:Abstract Effects of lubrication and Sample dimension on compression property of fish–meat Gel were investigated using the modified Mooney–Rivlin equation. Cylindrical fish–meat Gels were cut into 26 mm in diameter and 10, 20 and 30 mm in heights, respectively. Lubrication of Samples led to the lower stress–strain curve. Stress at fracture was dependent on Sample dimension in non-lubricated test. In lubricated compression, the dependant was reduced and the agreement of stress–strain data for all Samples was observed at the low strain region. The similar results were observed in comparing compression properties of non-lubricated and lubricated fish–meat Gels according to the modified Mooney–Rivlin equation. In order to reduce friction problems in compression test of fish–meat Gels, the experiment setting can be suggested as follows: for the Samples with small dimensions, the surface of Gel Sample has to be lubricated, or Gel Samples have to be made with large dimensions.
Sarah Cianférani - One of the best experts on this subject based on the ideXlab platform.
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Tube-Gel: A Fast and Effective Sample Preparation Method for High-Throughput Quantitative Proteomics
2019Co-Authors: Leslie Muller, Luc Fornecker, Sarah Cianférani, Christine CarapitoAbstract:Sample preparation is a key step in proteomics workflows. Tube-Gel (TG) is a fast and repeatable Sample preparation method that consists in the instantaneous trapping of the Sample in a polyacrylamide Gel matrix. It takes advantage of in-Gel Sample preparations by allowing the use of high concentrations of sodium-dodecyl sulfate but avoids the time-consuming step of electrophoresis. Therefore, TG limits the Sample handling and is thus particularly suitable for high-throughput quantitative proteomics when large Sample numbers have to be processed, as it is often the case in biomarker research and clinical proteomics projects.
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extended investigation of tube Gel Sample preparation a versatile and simple choice for high throughput quantitative proteomics
Scientific Reports, 2018Co-Authors: Leslie Muller, Luc Fornecker, Sarah Cianférani, Marie Chion, Alain Van Dorsselaer, Thierry Rabilloud, Christine CarapitoAbstract:Sample preparation for quantitative proteomics is a crucial step to ensure the repeatability and the accuracy of the results. However, there is no universal method compatible with the wide variety of protein extraction buffers currently used. We have recently demonstrated the compatibility of tube-Gel with SDS-based buffers and its efficiency for label-free quantitative proteomics by comparing it to stacking Gel and liquid digestion. Here, we investigated the compatibility of tube-Gel with alternatives to SDS-based buffers allowing notably the extraction of proteins in various pH conditions. We also explored the use of photopolymerization to extend the number of possibilities, as it is compatible with a wide range of pH and is non-oxidative. To achieve this goal, we compared six extraction buffers in combination with two polymerization conditions to further optimize the tube-Gel protocol and evaluate its versatility. Identification and quantitative results demonstrated the compatibility of tube-Gel with all tested conditions by overall raising quite comparable results. In conclusion, tube-Gel is a versatile and simple Sample preparation method for large-scale quantitative proteomics applications. Complete datasets are available via ProteomeXchange with identifier PXD008656.
