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Huajun Wang - One of the best experts on this subject based on the ideXlab platform.

  • Sustained Release SDF-1α/TGF-β1-Loaded Silk Fibroin-Porous Gelatin Scaffold Promotes Cartilage Repair.
    ACS applied materials & interfaces, 2019
    Co-Authors: Yuanfeng Chen, Huang Shusen, Chun-wai Wade Suen, Xin Cheng, Huige Hou, Guorong She, Huan-tian Zhang, Huajun Wang
    Abstract:

    Continuous delivery of growth factors to the injury site is crucial to creating a favorable microenvironment for cartilage injury repair. In the present study, we fabricated a novel sustained-release Scaffold, stromal-derived factor-1α (SDF-1α)/transforming growth factor-β1 (TGF-β1)-loaded silk fibroin-porous Gelatin Scaffold (GSTS). GSTS persistently releases SDF-1α and TGF-β1, which enhance cartilage repair by facilitating cell homing and chondrogenic differentiation. Scanning electron microscopy showed that GSTS is a porous microstructure and the protein release assay demonstrated the sustainable release of SDF-1α and TGF-β1 from GSTS. Bone marrow-derived mesenchymal stem cells (MSCs) maintain high in vitro cell activity and excellent cell distribution and phenotype after seeding into GSTS. Furthermore, MSCs acquired enhanced chondrogenic differentiation capability in the TGF-β1-loaded Scaffolds (GSTS or GST: loading TGF-β1 only) and the conditioned medium from SDF-1α-loaded Scaffolds (GSTS or GSS: loa...

  • sustained release sdf 1α tgf β1 loaded silk fibroin porous Gelatin Scaffold promotes cartilage repair
    ACS Applied Materials & Interfaces, 2019
    Co-Authors: Yuanfeng Chen, Chun-wai Wade Suen, Xin Cheng, Huige Hou, Guorong She, Huan-tian Zhang, Huajun Wang, Shusen Huang, Xiaofei Zheng, Zhengang Zha
    Abstract:

    Continuous delivery of growth factors to the injury site is crucial to creating a favorable microenvironment for cartilage injury repair. In the present study, we fabricated a novel sustained-release Scaffold, stromal-derived factor-1α (SDF-1α)/transforming growth factor-β1 (TGF-β1)-loaded silk fibroin-porous Gelatin Scaffold (GSTS). GSTS persistently releases SDF-1α and TGF-β1, which enhance cartilage repair by facilitating cell homing and chondrogenic differentiation. Scanning electron microscopy showed that GSTS is a porous microstructure and the protein release assay demonstrated the sustainable release of SDF-1α and TGF-β1 from GSTS. Bone marrow-derived mesenchymal stem cells (MSCs) maintain high in vitro cell activity and excellent cell distribution and phenotype after seeding into GSTS. Furthermore, MSCs acquired enhanced chondrogenic differentiation capability in the TGF-β1-loaded Scaffolds (GSTS or GST: loading TGF-β1 only) and the conditioned medium from SDF-1α-loaded Scaffolds (GSTS or GSS: loa...

Yuanfeng Chen - One of the best experts on this subject based on the ideXlab platform.

  • Sustained Release SDF-1α/TGF-β1-Loaded Silk Fibroin-Porous Gelatin Scaffold Promotes Cartilage Repair.
    ACS applied materials & interfaces, 2019
    Co-Authors: Yuanfeng Chen, Huang Shusen, Chun-wai Wade Suen, Xin Cheng, Huige Hou, Guorong She, Huan-tian Zhang, Huajun Wang
    Abstract:

    Continuous delivery of growth factors to the injury site is crucial to creating a favorable microenvironment for cartilage injury repair. In the present study, we fabricated a novel sustained-release Scaffold, stromal-derived factor-1α (SDF-1α)/transforming growth factor-β1 (TGF-β1)-loaded silk fibroin-porous Gelatin Scaffold (GSTS). GSTS persistently releases SDF-1α and TGF-β1, which enhance cartilage repair by facilitating cell homing and chondrogenic differentiation. Scanning electron microscopy showed that GSTS is a porous microstructure and the protein release assay demonstrated the sustainable release of SDF-1α and TGF-β1 from GSTS. Bone marrow-derived mesenchymal stem cells (MSCs) maintain high in vitro cell activity and excellent cell distribution and phenotype after seeding into GSTS. Furthermore, MSCs acquired enhanced chondrogenic differentiation capability in the TGF-β1-loaded Scaffolds (GSTS or GST: loading TGF-β1 only) and the conditioned medium from SDF-1α-loaded Scaffolds (GSTS or GSS: loa...

  • sustained release sdf 1α tgf β1 loaded silk fibroin porous Gelatin Scaffold promotes cartilage repair
    ACS Applied Materials & Interfaces, 2019
    Co-Authors: Yuanfeng Chen, Chun-wai Wade Suen, Xin Cheng, Huige Hou, Guorong She, Huan-tian Zhang, Huajun Wang, Shusen Huang, Xiaofei Zheng, Zhengang Zha
    Abstract:

    Continuous delivery of growth factors to the injury site is crucial to creating a favorable microenvironment for cartilage injury repair. In the present study, we fabricated a novel sustained-release Scaffold, stromal-derived factor-1α (SDF-1α)/transforming growth factor-β1 (TGF-β1)-loaded silk fibroin-porous Gelatin Scaffold (GSTS). GSTS persistently releases SDF-1α and TGF-β1, which enhance cartilage repair by facilitating cell homing and chondrogenic differentiation. Scanning electron microscopy showed that GSTS is a porous microstructure and the protein release assay demonstrated the sustainable release of SDF-1α and TGF-β1 from GSTS. Bone marrow-derived mesenchymal stem cells (MSCs) maintain high in vitro cell activity and excellent cell distribution and phenotype after seeding into GSTS. Furthermore, MSCs acquired enhanced chondrogenic differentiation capability in the TGF-β1-loaded Scaffolds (GSTS or GST: loading TGF-β1 only) and the conditioned medium from SDF-1α-loaded Scaffolds (GSTS or GSS: loa...

Siriporn Damrongsakkul - One of the best experts on this subject based on the ideXlab platform.

  • balanced electrostatic blending approach an alternative to chemical crosslinking of thai silk fibroin Gelatin Scaffold
    International Journal of Biological Macromolecules, 2012
    Co-Authors: Panida Jetbumpenkul, Phakdee Amornsudthiwat, Sorada Kanokpanont, Siriporn Damrongsakkul
    Abstract:

    In tissue engineering, chemical crosslinking is widely used for conjugating two or more biomaterials to mainly control biodegradability and strength. For example, Thai silk fibroin/Gelatin Scaffold will offer mechanical strength from Thai silk fibroin and cell attraction from Gelatin. However, chemical crosslinking requires crosslinking agent which could potentially pose negative impact from remaining trace amount of chemicals especially in medical application. Here we present an alternative approach to chemical crosslinking—a balance electrostatic blending approach. In this approach, two opposite charge biomaterials were selected for blending, with different ratios. Both materials were bound together with electrostatic force. The maximum binding was achieved when mixture electric potential approaches zero. In this work, we compared this approach with traditionally chemical crosslinking in terms of physical appearance, binding effectiveness, mechanical strength (in dry/wet conditions), in vitro biodegradation, and cell proliferation. We found that 50/50 weight ratio of Thai silk fibroin/Gelatin Scaffold had almost comparable properties to chemical crosslinked Scaffold. It has similar appearance, binding effectiveness, and affinity for cell proliferation. For mechanical properties, even this approach yields lower dry compressive modulus compared with chemical crosslinking. But in wet condition, the compressive modulus from both methods is similar. However, the biodegradation time of non-crosslinked Scaffolds is slightly faster than that of chemical crosslinked ones. These results demonstrate that a balance electrostatic approach is an alternative approach to chemical crosslinking when there is a concern of remaining trace amount of crosslinking agent in medical application.

  • Balanced electrostatic blending approach – An alternative to chemical crosslinking of Thai silk fibroin/Gelatin Scaffold
    International journal of biological macromolecules, 2011
    Co-Authors: Panida Jetbumpenkul, Phakdee Amornsudthiwat, Sorada Kanokpanont, Siriporn Damrongsakkul
    Abstract:

    In tissue engineering, chemical crosslinking is widely used for conjugating two or more biomaterials to mainly control biodegradability and strength. For example, Thai silk fibroin/Gelatin Scaffold will offer mechanical strength from Thai silk fibroin and cell attraction from Gelatin. However, chemical crosslinking requires crosslinking agent which could potentially pose negative impact from remaining trace amount of chemicals especially in medical application. Here we present an alternative approach to chemical crosslinking—a balance electrostatic blending approach. In this approach, two opposite charge biomaterials were selected for blending, with different ratios. Both materials were bound together with electrostatic force. The maximum binding was achieved when mixture electric potential approaches zero. In this work, we compared this approach with traditionally chemical crosslinking in terms of physical appearance, binding effectiveness, mechanical strength (in dry/wet conditions), in vitro biodegradation, and cell proliferation. We found that 50/50 weight ratio of Thai silk fibroin/Gelatin Scaffold had almost comparable properties to chemical crosslinked Scaffold. It has similar appearance, binding effectiveness, and affinity for cell proliferation. For mechanical properties, even this approach yields lower dry compressive modulus compared with chemical crosslinking. But in wet condition, the compressive modulus from both methods is similar. However, the biodegradation time of non-crosslinked Scaffolds is slightly faster than that of chemical crosslinked ones. These results demonstrate that a balance electrostatic approach is an alternative approach to chemical crosslinking when there is a concern of remaining trace amount of crosslinking agent in medical application.

Hongquan Zhang - One of the best experts on this subject based on the ideXlab platform.

  • bone tissue engineering using bone marrow stromal cells and an injectable sodium alginate Gelatin Scaffold
    Journal of Biomedical Materials Research Part A, 2012
    Co-Authors: Yang Xia, Fang Mei, Yongli Duan, Ying Gao, Zhuo Xiong, Ting Zhang, Hongquan Zhang
    Abstract:

    To investigate the potential application of bone marrow stromal cells (BMSCs) and an injectable sodium alginate/Gelatin Scaffold for bone tissue engineering (BTE). The phenotype of osteogenic BMSCs was examined by mineralized nodules formation and type I collagen expression. Cell proliferation was evaluated by MTT assay. The biocompatibility of Scaffold and osteogenic cells were examined by hematoxylin and eosin (H&E) staining. Ectopic bone formation as well as closure of rabbit calvarial critical-sized defects following Scaffold-cell implantation were analyzed by histological examination and computed tomography (CT) scanning. Spindle-shaped osteogenic cells of high purity were derived from BMSCs. The osteogenic cells and sodium alginate/Gelatin (2:3) Scaffold presented fine biocompatibility following cross-linking with 0.6% of CaCl2. After implantation, the Scaffold-cell construct promoted both ectopic bone formation and bone healing in the rabbit calvarial critical-sized defect model. Our data demonstrated that the sodium alginate/Gelatin Scaffold could be a suitable biomaterial for bone engineering, and the Scaffold-osteogenic cells construct is a promising alternative approach for the bone healing process. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A:, 2012.

  • Bone tissue engineering using bone marrow stromal cells and an injectable sodium alginate/Gelatin Scaffold.
    Journal of biomedical materials research. Part A, 2012
    Co-Authors: Yang Xia, Fang Mei, Yongli Duan, Ying Gao, Zhuo Xiong, Ting Zhang, Hongquan Zhang
    Abstract:

    To investigate the potential application of bone marrow stromal cells (BMSCs) and an injectable sodium alginate/Gelatin Scaffold for bone tissue engineering (BTE). The phenotype of osteogenic BMSCs was examined by mineralized nodules formation and type I collagen expression. Cell proliferation was evaluated by MTT assay. The biocompatibility of Scaffold and osteogenic cells were examined by hematoxylin and eosin (H&E) staining. Ectopic bone formation as well as closure of rabbit calvarial critical-sized defects following Scaffold-cell implantation were analyzed by histological examination and computed tomography (CT) scanning. Spindle-shaped osteogenic cells of high purity were derived from BMSCs. The osteogenic cells and sodium alginate/Gelatin (2:3) Scaffold presented fine biocompatibility following cross-linking with 0.6% of CaCl2. After implantation, the Scaffold-cell construct promoted both ectopic bone formation and bone healing in the rabbit calvarial critical-sized defect model. Our data demonstrated that the sodium alginate/Gelatin Scaffold could be a suitable biomaterial for bone engineering, and the Scaffold-osteogenic cells construct is a promising alternative approach for the bone healing process. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A:, 2012.

Zhengang Zha - One of the best experts on this subject based on the ideXlab platform.

  • sustained release sdf 1α tgf β1 loaded silk fibroin porous Gelatin Scaffold promotes cartilage repair
    ACS Applied Materials & Interfaces, 2019
    Co-Authors: Yuanfeng Chen, Chun-wai Wade Suen, Xin Cheng, Huige Hou, Guorong She, Huan-tian Zhang, Huajun Wang, Shusen Huang, Xiaofei Zheng, Zhengang Zha
    Abstract:

    Continuous delivery of growth factors to the injury site is crucial to creating a favorable microenvironment for cartilage injury repair. In the present study, we fabricated a novel sustained-release Scaffold, stromal-derived factor-1α (SDF-1α)/transforming growth factor-β1 (TGF-β1)-loaded silk fibroin-porous Gelatin Scaffold (GSTS). GSTS persistently releases SDF-1α and TGF-β1, which enhance cartilage repair by facilitating cell homing and chondrogenic differentiation. Scanning electron microscopy showed that GSTS is a porous microstructure and the protein release assay demonstrated the sustainable release of SDF-1α and TGF-β1 from GSTS. Bone marrow-derived mesenchymal stem cells (MSCs) maintain high in vitro cell activity and excellent cell distribution and phenotype after seeding into GSTS. Furthermore, MSCs acquired enhanced chondrogenic differentiation capability in the TGF-β1-loaded Scaffolds (GSTS or GST: loading TGF-β1 only) and the conditioned medium from SDF-1α-loaded Scaffolds (GSTS or GSS: loa...