The Experts below are selected from a list of 10908 Experts worldwide ranked by ideXlab platform

Xuesi Chen - One of the best experts on this subject based on the ideXlab platform.

  • a strategy of killing three birds with one stone for cancer therapy through regulating the tumor microenvironment by h2o2 responsive Gene Delivery System
    ACS Applied Materials & Interfaces, 2019
    Co-Authors: Jie Chen, Huayu Tian, Xuesi Chen, Huapan Fang
    Abstract:

    Constructing an efficient in vivo Gene Delivery System has always been extremely challenging. Herein, a highly efficient H2O2-responsive in vivo polycationic Gene Delivery System is developed for t...

  • a ph responsive detachable peg shielding strategy for Gene Delivery System in cancer therapy
    Biomacromolecules, 2017
    Co-Authors: Xiuwen Guan, Jie Chen, Huayu Tian, Tinghong Wang, Xuesi Chen
    Abstract:

    In this study, a pH-responsive detachable polyethylene glycol (PEG) shielding strategy was designed for Gene Delivery in cancer therapy. Polyethylenimine/DNA complex (PEI/DNA) was in situ shielded by aldehyde group-modified PEG derivatives. The aldehyde groups of PEG could react with the amino groups of PEI by Schiff base reaction. The Schiff base bond was stable in neutral pH but labile in slightly acidic pH, which made the PEG sheddable in tumors. PEG-coated nanoparticles (NPs) had distinct advantages compared to their mPEG counterpart, possessing decreased zeta potential, more compressed size, and enhanced stability. PEG/PEI/DNA NPs showed not only high tumor cell uptake and transfection efficiency in vitro but also efficient accumulation and Gene expression in solid tumors in vivo. This pH-responsive detachable PEG shielding System has the potential to be applied to other polycationic nanoparticles that contain amino groups on their surfaces, which will have broad prospects in cancer therapy.

  • ultrasensitive ph triggered charge size dual rebound Gene Delivery System
    Nano Letters, 2016
    Co-Authors: Xiuwen Guan, Jie Chen, Huayu Tian, Xuesi Chen
    Abstract:

    A facile strategy is developed to construct an ultrasensitive pH triggered charge/size dual-rebound Gene Delivery System for efficient tumor treatment. The therapeutic Gene is complexed by polyethylenimine (PEI) and poly-l-glutamate (PLG), further in situ tightened by aldehyde modified polyethylene glycol (PEG) via Schiff base reaction. The Generated Schiff base bonds are stable in neutral pH but cleavable in tumor extracellular pH. This Gene Delivery System possesses following favorable properties: (1) the tunable Gene Delivery System is constructed by chemical bench-free “green” and fast process which is favored by clinician, (2) PEG cross-linking shields the surface positive charges and tightens the complex particles, leading to decreased cytotoxicity, improved stability, and prolonged circulation, (3) PEG shielding can be rapidly peeled off by acidic pH as soon as arriving tumors, (4) dual charge/size ultrasensitively rebounding to higher positive potential and bigger size enhances tumor cell uptake e...

  • Ultrasensitive pH Triggered Charge/Size Dual-Rebound Gene Delivery System
    Nano Letters, 2016
    Co-Authors: Xiuwen Guan, Jie Chen, Huayu Tian, Xuesi Chen
    Abstract:

    A facile strategy is developed to construct an ultrasensitive pH triggered charge/size dual-rebound Gene Delivery System for efficient tumor treatment. The therapeutic Gene is complexed by polyethylenimine (PEI) and poly-l-glutamate (PLG), further in situ tightened by aldehyde modified polyethylene glycol (PEG) via Schiff base reaction. The Generated Schiff base bonds are stable in neutral pH but cleavable in tumor extracellular pH. This Gene Delivery System possesses following favorable properties: (1) the tunable Gene Delivery System is constructed by chemical bench-free “green” and fast process which is favored by clinician, (2) PEG cross-linking shields the surface positive charges and tightens the complex particles, leading to decreased cytotoxicity, improved stability, and prolonged circulation, (3) PEG shielding can be rapidly peeled off by acidic pH as soon as arriving tumors, (4) dual charge/size ultrasensitively rebounding to higher positive potential and bigger size enhances tumor cell uptake e...

Hideyoshi Harashima - One of the best experts on this subject based on the ideXlab platform.

  • A Novel Nonviral Gene Delivery System: Multifunctional Envelope-Type Nano Device
    Advances in Biochemical Engineering \ Biotechnology, 2009
    Co-Authors: Hiroto Hatakeyama, Hidetaka Akita, Kentaro Kogure, Hideyoshi Harashima
    Abstract:

    In this review we introduce a new concept for developing a nonviral Gene Delivery System which we call “Programmed Packaging.” Based on this concept, we succeeded in developing a multifunctional envelope-type nano device (MEND), which exerts high transfection activities equivalent to those of an adenovirus in a dividing cell. The use of MEND has been extended to in vivo applications. PEG/peptide/DOPE ternary conjugate (PPD)-MEND, a new in vivo Gene Delivery System for the targeting of tumor cells that dissociates surface-modified PEG in tumor tissue by matrix metalloproteinase (MMP) and exerts significant transfection activities, was developed. In parallel with the development of MEND, a quantitative Gene Delivery System, Confocal Image-assisted 3-dimensionally integrated quantification (CIDIQ), also was developed. This method identified the rate-limiting step of the nonviral Gene Delivery System by comparing it with adenoviral-mediated Gene Delivery. The results of this analysis provide a new direction for the development of rational nonviral Gene Delivery Systems.

  • multifunctional envelope type nano device mend as a non viral Gene Delivery System
    Advanced Drug Delivery Reviews, 2008
    Co-Authors: Kentaro Kogure, Yuma Yamada, Hidetaka Akita, Hideyoshi Harashima
    Abstract:

    Abstract In this review, we describe a key role of octaarginine (R8) in developing our new concept of “Programmed Packaging”, by which we succeeded in creating a multifunctional envelope-type nano device (MEND) as a non-viral Gene-Delivery System. This concept can be applied not only to nuclear targeting of plasmid DNA (pDNA) but also to cytosolic Delivery of functional nucleic acids such as oligonucleotides or siRNA. This concept has been extended to other organelles such as mitochondria as a foundation for innovative nanomedicine. Finally, we discuss the rate-limiting step in Gene Delivery by comparing non-viral and viral Gene Delivery Systems, which clearly indicates the importance of nuclear disposition of pDNA for efficient transfection.

  • development of multifunctional envelope type artificial viral like Gene Delivery System
    Yakugaku Zasshi-journal of The Pharmaceutical Society of Japan, 2007
    Co-Authors: Hideyoshi Harashima, Kentaro Kogure, Yuma Yamada, Hidetaka Akita, Hiroyuki Kamiya
    Abstract:

    : This review introduces a new concept "Programmed Packaging" to develop a non-viral Gene Delivery System. Based on this concept, multifunctional envelope type nano devices (MEND) were developed for in vitro, in situ and in vivo conditions. A quantitative study to identify a rate limiting step in intracellular trafficking was also shown between viral and non-viral vectors, which indicated an important role of controlled intranuclear disposition for development a safe and efficient non-viral Gene Delivery System. This review will provide a future direction of non-viral Gene Delivery System.

  • development of a novel Systemic Gene Delivery System for cancer therapy with a tumor specific cleavable peg lipid
    Gene Therapy, 2007
    Co-Authors: Hiroto Hatakeyama, Kentaro Kogure, Hidetaka Akita, Masaharu Ueno, Motoi Oishi, Yukio Nagasaki, Y Kihira, Hideo Kobayashi, Hiroshi Kikuchi, Hideyoshi Harashima
    Abstract:

    For successful cancer Gene therapy via intravenous (i.v.) administration, it is essential to optimize the stability of carriers in the Systemic circulation and the cellular association after the accumulation of the carrier in tumor tissue. However, a dilemma exists regarding the use of poly(ethylene glycol) (PEG), which is useful for conferring stability in the Systemic circulation, but is undesirable for the cellular uptake and the following processes. We report the development of a PEG-peptide-lipid ternary conjugate (PEG-Peptide-DOPE conjugate (PPD)). In this strategy, the PEG is removed from the carriers via cleavage by a matrix metalloproteinase (MMP), which is specifically expressed in tumor tissues. An in vitro study revealed that the PPD-modified Gene carrier (Multifunctional Envelope-type Nano Device: MEND) exhibited pDNA expression activity that was dependent on the MMP expression level in the host cells. In vivo studies further revealed that the PPD was potent in stabilizing MEND in the Systemic circulation and facilitating tumor accumulation. Moreover, the i.v. administration of PPD or PEG/PPD dually-modified MEND resulted in the stimulation of pDNA expression in tumor tissue, as compared with a conventional PEG-modified MEND. Thus, MEND modified with PPD is a promising device, which has the potential to make in vivo cancer Gene therapy achievable.

  • development of a non viral multifunctional envelope type nano device by a novel lipid film hydration method
    Journal of Controlled Release, 2004
    Co-Authors: Kentaro Kogure, Shiroh Futaki, Rumiko Moriguchi, Kentaro Sasaki, Masaharu Ueno, Hideyoshi Harashima
    Abstract:

    Abstract The development of a multifunctional envelope-type nano device (MEND) for use in a non-viral Gene Delivery System using a novel lipid film hydration method is described. This packaging method involves three steps: (i) DNA condensation with a polycation, (ii) lipid film hydration for the electrostatic binding of the condensed DNA, and (iii) sonication to package the condensed DNA with lipids. For DNA condensation, the optimum ratio of DNA to poly- l -lysine (PLL) was determined by titrating DNA and PLL. The condensed DNA/PLL complex (DPC) was coated with a lipid bilayer by lipid film hydration followed by sonication, which was confirmed by sucrose density gradient centrifugation. Packaging of DPC with lipids increased the transfection activity 10-fold over that of DPC. MEND, having octaarginine on the envelope as a device for membrane penetration to enhance cellular uptake, showed a 1000-fold higher transfection activity than DPC. The MEND developed in this study represents a promising non-viral Gene Delivery System.

Huayu Tian - One of the best experts on this subject based on the ideXlab platform.

  • a strategy of killing three birds with one stone for cancer therapy through regulating the tumor microenvironment by h2o2 responsive Gene Delivery System
    ACS Applied Materials & Interfaces, 2019
    Co-Authors: Jie Chen, Huayu Tian, Xuesi Chen, Huapan Fang
    Abstract:

    Constructing an efficient in vivo Gene Delivery System has always been extremely challenging. Herein, a highly efficient H2O2-responsive in vivo polycationic Gene Delivery System is developed for t...

  • a ph responsive detachable peg shielding strategy for Gene Delivery System in cancer therapy
    Biomacromolecules, 2017
    Co-Authors: Xiuwen Guan, Jie Chen, Huayu Tian, Tinghong Wang, Xuesi Chen
    Abstract:

    In this study, a pH-responsive detachable polyethylene glycol (PEG) shielding strategy was designed for Gene Delivery in cancer therapy. Polyethylenimine/DNA complex (PEI/DNA) was in situ shielded by aldehyde group-modified PEG derivatives. The aldehyde groups of PEG could react with the amino groups of PEI by Schiff base reaction. The Schiff base bond was stable in neutral pH but labile in slightly acidic pH, which made the PEG sheddable in tumors. PEG-coated nanoparticles (NPs) had distinct advantages compared to their mPEG counterpart, possessing decreased zeta potential, more compressed size, and enhanced stability. PEG/PEI/DNA NPs showed not only high tumor cell uptake and transfection efficiency in vitro but also efficient accumulation and Gene expression in solid tumors in vivo. This pH-responsive detachable PEG shielding System has the potential to be applied to other polycationic nanoparticles that contain amino groups on their surfaces, which will have broad prospects in cancer therapy.

  • ultrasensitive ph triggered charge size dual rebound Gene Delivery System
    Nano Letters, 2016
    Co-Authors: Xiuwen Guan, Jie Chen, Huayu Tian, Xuesi Chen
    Abstract:

    A facile strategy is developed to construct an ultrasensitive pH triggered charge/size dual-rebound Gene Delivery System for efficient tumor treatment. The therapeutic Gene is complexed by polyethylenimine (PEI) and poly-l-glutamate (PLG), further in situ tightened by aldehyde modified polyethylene glycol (PEG) via Schiff base reaction. The Generated Schiff base bonds are stable in neutral pH but cleavable in tumor extracellular pH. This Gene Delivery System possesses following favorable properties: (1) the tunable Gene Delivery System is constructed by chemical bench-free “green” and fast process which is favored by clinician, (2) PEG cross-linking shields the surface positive charges and tightens the complex particles, leading to decreased cytotoxicity, improved stability, and prolonged circulation, (3) PEG shielding can be rapidly peeled off by acidic pH as soon as arriving tumors, (4) dual charge/size ultrasensitively rebounding to higher positive potential and bigger size enhances tumor cell uptake e...

  • Ultrasensitive pH Triggered Charge/Size Dual-Rebound Gene Delivery System
    Nano Letters, 2016
    Co-Authors: Xiuwen Guan, Jie Chen, Huayu Tian, Xuesi Chen
    Abstract:

    A facile strategy is developed to construct an ultrasensitive pH triggered charge/size dual-rebound Gene Delivery System for efficient tumor treatment. The therapeutic Gene is complexed by polyethylenimine (PEI) and poly-l-glutamate (PLG), further in situ tightened by aldehyde modified polyethylene glycol (PEG) via Schiff base reaction. The Generated Schiff base bonds are stable in neutral pH but cleavable in tumor extracellular pH. This Gene Delivery System possesses following favorable properties: (1) the tunable Gene Delivery System is constructed by chemical bench-free “green” and fast process which is favored by clinician, (2) PEG cross-linking shields the surface positive charges and tightens the complex particles, leading to decreased cytotoxicity, improved stability, and prolonged circulation, (3) PEG shielding can be rapidly peeled off by acidic pH as soon as arriving tumors, (4) dual charge/size ultrasensitively rebounding to higher positive potential and bigger size enhances tumor cell uptake e...

Jie Chen - One of the best experts on this subject based on the ideXlab platform.

  • a strategy of killing three birds with one stone for cancer therapy through regulating the tumor microenvironment by h2o2 responsive Gene Delivery System
    ACS Applied Materials & Interfaces, 2019
    Co-Authors: Jie Chen, Huayu Tian, Xuesi Chen, Huapan Fang
    Abstract:

    Constructing an efficient in vivo Gene Delivery System has always been extremely challenging. Herein, a highly efficient H2O2-responsive in vivo polycationic Gene Delivery System is developed for t...

  • a ph responsive detachable peg shielding strategy for Gene Delivery System in cancer therapy
    Biomacromolecules, 2017
    Co-Authors: Xiuwen Guan, Jie Chen, Huayu Tian, Tinghong Wang, Xuesi Chen
    Abstract:

    In this study, a pH-responsive detachable polyethylene glycol (PEG) shielding strategy was designed for Gene Delivery in cancer therapy. Polyethylenimine/DNA complex (PEI/DNA) was in situ shielded by aldehyde group-modified PEG derivatives. The aldehyde groups of PEG could react with the amino groups of PEI by Schiff base reaction. The Schiff base bond was stable in neutral pH but labile in slightly acidic pH, which made the PEG sheddable in tumors. PEG-coated nanoparticles (NPs) had distinct advantages compared to their mPEG counterpart, possessing decreased zeta potential, more compressed size, and enhanced stability. PEG/PEI/DNA NPs showed not only high tumor cell uptake and transfection efficiency in vitro but also efficient accumulation and Gene expression in solid tumors in vivo. This pH-responsive detachable PEG shielding System has the potential to be applied to other polycationic nanoparticles that contain amino groups on their surfaces, which will have broad prospects in cancer therapy.

  • ultrasensitive ph triggered charge size dual rebound Gene Delivery System
    Nano Letters, 2016
    Co-Authors: Xiuwen Guan, Jie Chen, Huayu Tian, Xuesi Chen
    Abstract:

    A facile strategy is developed to construct an ultrasensitive pH triggered charge/size dual-rebound Gene Delivery System for efficient tumor treatment. The therapeutic Gene is complexed by polyethylenimine (PEI) and poly-l-glutamate (PLG), further in situ tightened by aldehyde modified polyethylene glycol (PEG) via Schiff base reaction. The Generated Schiff base bonds are stable in neutral pH but cleavable in tumor extracellular pH. This Gene Delivery System possesses following favorable properties: (1) the tunable Gene Delivery System is constructed by chemical bench-free “green” and fast process which is favored by clinician, (2) PEG cross-linking shields the surface positive charges and tightens the complex particles, leading to decreased cytotoxicity, improved stability, and prolonged circulation, (3) PEG shielding can be rapidly peeled off by acidic pH as soon as arriving tumors, (4) dual charge/size ultrasensitively rebounding to higher positive potential and bigger size enhances tumor cell uptake e...

  • Ultrasensitive pH Triggered Charge/Size Dual-Rebound Gene Delivery System
    Nano Letters, 2016
    Co-Authors: Xiuwen Guan, Jie Chen, Huayu Tian, Xuesi Chen
    Abstract:

    A facile strategy is developed to construct an ultrasensitive pH triggered charge/size dual-rebound Gene Delivery System for efficient tumor treatment. The therapeutic Gene is complexed by polyethylenimine (PEI) and poly-l-glutamate (PLG), further in situ tightened by aldehyde modified polyethylene glycol (PEG) via Schiff base reaction. The Generated Schiff base bonds are stable in neutral pH but cleavable in tumor extracellular pH. This Gene Delivery System possesses following favorable properties: (1) the tunable Gene Delivery System is constructed by chemical bench-free “green” and fast process which is favored by clinician, (2) PEG cross-linking shields the surface positive charges and tightens the complex particles, leading to decreased cytotoxicity, improved stability, and prolonged circulation, (3) PEG shielding can be rapidly peeled off by acidic pH as soon as arriving tumors, (4) dual charge/size ultrasensitively rebounding to higher positive potential and bigger size enhances tumor cell uptake e...

  • A novel Gene Delivery System using magnetic nanodarts
    2009 IEEE NIH Life Science Systems and Applications Workshop, 2009
    Co-Authors: Weibing Lu, Peng Xu, James Xing, Jian Zhang, Jie Chen
    Abstract:

    One aspect of Gene therapy is the efficient transfection of a cell population with DNAs or RNAs (including siRNAs) in order to modulate the expression of Genes, to produce cell types for use in Gene therapy, or to meet other very important unmet medical needs. Gene therapy has a huge potential in modifying the blueprints of a human cell and giving additional tools for modern medicine. Gene therapy also offers hope in combating lethal Genetic diseases, cancer and certain infectious diseases. According to the report ldquoMeeting the challenge: U.S. Industry faces the 21st century: The U.S. biotechnology industryrdquo, the office of technology policy, U.S. Department of Commerce predicted the growth of this industrial sector to be an estimated $5 Billion within the next decade.

Xiuwen Guan - One of the best experts on this subject based on the ideXlab platform.

  • a ph responsive detachable peg shielding strategy for Gene Delivery System in cancer therapy
    Biomacromolecules, 2017
    Co-Authors: Xiuwen Guan, Jie Chen, Huayu Tian, Tinghong Wang, Xuesi Chen
    Abstract:

    In this study, a pH-responsive detachable polyethylene glycol (PEG) shielding strategy was designed for Gene Delivery in cancer therapy. Polyethylenimine/DNA complex (PEI/DNA) was in situ shielded by aldehyde group-modified PEG derivatives. The aldehyde groups of PEG could react with the amino groups of PEI by Schiff base reaction. The Schiff base bond was stable in neutral pH but labile in slightly acidic pH, which made the PEG sheddable in tumors. PEG-coated nanoparticles (NPs) had distinct advantages compared to their mPEG counterpart, possessing decreased zeta potential, more compressed size, and enhanced stability. PEG/PEI/DNA NPs showed not only high tumor cell uptake and transfection efficiency in vitro but also efficient accumulation and Gene expression in solid tumors in vivo. This pH-responsive detachable PEG shielding System has the potential to be applied to other polycationic nanoparticles that contain amino groups on their surfaces, which will have broad prospects in cancer therapy.

  • ultrasensitive ph triggered charge size dual rebound Gene Delivery System
    Nano Letters, 2016
    Co-Authors: Xiuwen Guan, Jie Chen, Huayu Tian, Xuesi Chen
    Abstract:

    A facile strategy is developed to construct an ultrasensitive pH triggered charge/size dual-rebound Gene Delivery System for efficient tumor treatment. The therapeutic Gene is complexed by polyethylenimine (PEI) and poly-l-glutamate (PLG), further in situ tightened by aldehyde modified polyethylene glycol (PEG) via Schiff base reaction. The Generated Schiff base bonds are stable in neutral pH but cleavable in tumor extracellular pH. This Gene Delivery System possesses following favorable properties: (1) the tunable Gene Delivery System is constructed by chemical bench-free “green” and fast process which is favored by clinician, (2) PEG cross-linking shields the surface positive charges and tightens the complex particles, leading to decreased cytotoxicity, improved stability, and prolonged circulation, (3) PEG shielding can be rapidly peeled off by acidic pH as soon as arriving tumors, (4) dual charge/size ultrasensitively rebounding to higher positive potential and bigger size enhances tumor cell uptake e...

  • Ultrasensitive pH Triggered Charge/Size Dual-Rebound Gene Delivery System
    Nano Letters, 2016
    Co-Authors: Xiuwen Guan, Jie Chen, Huayu Tian, Xuesi Chen
    Abstract:

    A facile strategy is developed to construct an ultrasensitive pH triggered charge/size dual-rebound Gene Delivery System for efficient tumor treatment. The therapeutic Gene is complexed by polyethylenimine (PEI) and poly-l-glutamate (PLG), further in situ tightened by aldehyde modified polyethylene glycol (PEG) via Schiff base reaction. The Generated Schiff base bonds are stable in neutral pH but cleavable in tumor extracellular pH. This Gene Delivery System possesses following favorable properties: (1) the tunable Gene Delivery System is constructed by chemical bench-free “green” and fast process which is favored by clinician, (2) PEG cross-linking shields the surface positive charges and tightens the complex particles, leading to decreased cytotoxicity, improved stability, and prolonged circulation, (3) PEG shielding can be rapidly peeled off by acidic pH as soon as arriving tumors, (4) dual charge/size ultrasensitively rebounding to higher positive potential and bigger size enhances tumor cell uptake e...