The Experts below are selected from a list of 70452 Experts worldwide ranked by ideXlab platform
Richard S Hanson - One of the best experts on this subject based on the ideXlab platform.
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soluble methane monooxygenase component b Gene Probe for identification of methanotrophs that rapidly degrade trichloroethylene
Applied and Environmental Microbiology, 1992Co-Authors: Hsienchyang Tsien, Richard S HansonAbstract:Restriction fragment length polymorphisms, Western blot (immunoblot) analysis, and fluorescence-labelled signature Probes were used for the characterization of methanotrophic bacteria as well as for the identification of methanotrophs which contained the soluble methane monooxygenase (MMO) Gene and were able to degrade trichloroethylene (TCE). The Gene encoding a soluble MMO component B protein from Methylosinus trichosporium OB3b was cloned. It contained a 2.2-kb EcoRI fragment. With this cloned component B Gene as Probe, methanotroph types I, II, and X and environmental and bioreactor samples were screened for the presence of the Gene encoding soluble MMO. Among twelve pure or mixed cultures, DNA fragments of seven methanotrophs hybridized with the soluble MMO B Gene Probe. When grown in media with limited copper, all of these bacteria degraded TCE. All of them are type II methanotrophs. The soluble MMO component B Gene of the type X methanotroph, Methylococcus capsulatus Bath, did not hybridize to the M. trichosporium OB3b soluble MMO component B Gene Probe, although M. capsulatus Baath also produces a soluble MMO.
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soluble methane monooxygenase component b Gene Probe for identification of methanotrophs that rapidly degrade trichloroethylene
Applied and Environmental Microbiology, 1992Co-Authors: Hsienchyang Tsien, Richard S HansonAbstract:Restriction fragment length polymorphisms, Western blot (immunoblot) analysis, and fluorescence-labelled signature Probes were used for the characterization of methanotrophic bacteria as well as for the identification of methanotrophs which contained the soluble methane monooxygenase (MMO) Gene and were able to degrade trichloroethylene (TCE). The Gene encoding a soluble MMO component B protein from Methylosinus trichosporium OB3b was cloned. It contained a 2.2-kb EcoRI fragment. With this cloned component B Gene as Probe, methanotroph types I, II, and X and environmental and bioreactor samples were screened for the presence of the Gene encoding soluble MMO. Among twelve pure or mixed cultures, DNA fragments of seven methanotrophs hybridized with the soluble MMO B Gene Probe. When grown in media with limited copper, all of these bacteria degraded TCE. All of them are type II methanotrophs. The soluble MMO component B Gene of the type X methanotroph, Methylococcus capsulatus Bath, did not hybridize to the M. trichosporium OB3b soluble MMO component B Gene Probe, although M. capsulatus Baath also produces a soluble MMO.
James B Heale - One of the best experts on this subject based on the ideXlab platform.
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restriction fragment length polymorphisms in mitochondrial dna and ribosomal rna Gene complexes as an aid to the characterization of species and sub species populations in the genus verticillium
Fems Microbiology Letters, 1992Co-Authors: Milton A Typas, Alison M Griffen, Brian W Bainbridge, James B HealeAbstract:Genomic DNA was extracted from seven species of Verticillium and digested with the restriction endonucleases EcoRI or HaeIII. Hybridization with an homologous V. albo-atrum ribosomal RNA Gene Probe revealed restriction fragment length polymorphisms (RFLPs) which could differentiate V. lateritium, V. lecanii, V. nigrescens, V. nubilum and V. tricorpus. Digestion with EcoRI did not provide RFLPs which could distinguish between V. albo-atrum and V. dahliae. Digestion of genomic and mitochondrial DNA with HaeIII showed distinctive patterns on ethidium bromide gels which allowed each species to be distinguished. Some intra-species variation in patterns occurred and a combination of mitochondrial and ribosomal RNA Gene complex RFLPs has potential as an aid for the characterization of species and sub-species populations in the Genes Verticillium.
Hsienchyang Tsien - One of the best experts on this subject based on the ideXlab platform.
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soluble methane monooxygenase component b Gene Probe for identification of methanotrophs that rapidly degrade trichloroethylene
Applied and Environmental Microbiology, 1992Co-Authors: Hsienchyang Tsien, Richard S HansonAbstract:Restriction fragment length polymorphisms, Western blot (immunoblot) analysis, and fluorescence-labelled signature Probes were used for the characterization of methanotrophic bacteria as well as for the identification of methanotrophs which contained the soluble methane monooxygenase (MMO) Gene and were able to degrade trichloroethylene (TCE). The Gene encoding a soluble MMO component B protein from Methylosinus trichosporium OB3b was cloned. It contained a 2.2-kb EcoRI fragment. With this cloned component B Gene as Probe, methanotroph types I, II, and X and environmental and bioreactor samples were screened for the presence of the Gene encoding soluble MMO. Among twelve pure or mixed cultures, DNA fragments of seven methanotrophs hybridized with the soluble MMO B Gene Probe. When grown in media with limited copper, all of these bacteria degraded TCE. All of them are type II methanotrophs. The soluble MMO component B Gene of the type X methanotroph, Methylococcus capsulatus Bath, did not hybridize to the M. trichosporium OB3b soluble MMO component B Gene Probe, although M. capsulatus Baath also produces a soluble MMO.
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soluble methane monooxygenase component b Gene Probe for identification of methanotrophs that rapidly degrade trichloroethylene
Applied and Environmental Microbiology, 1992Co-Authors: Hsienchyang Tsien, Richard S HansonAbstract:Restriction fragment length polymorphisms, Western blot (immunoblot) analysis, and fluorescence-labelled signature Probes were used for the characterization of methanotrophic bacteria as well as for the identification of methanotrophs which contained the soluble methane monooxygenase (MMO) Gene and were able to degrade trichloroethylene (TCE). The Gene encoding a soluble MMO component B protein from Methylosinus trichosporium OB3b was cloned. It contained a 2.2-kb EcoRI fragment. With this cloned component B Gene as Probe, methanotroph types I, II, and X and environmental and bioreactor samples were screened for the presence of the Gene encoding soluble MMO. Among twelve pure or mixed cultures, DNA fragments of seven methanotrophs hybridized with the soluble MMO B Gene Probe. When grown in media with limited copper, all of these bacteria degraded TCE. All of them are type II methanotrophs. The soluble MMO component B Gene of the type X methanotroph, Methylococcus capsulatus Bath, did not hybridize to the M. trichosporium OB3b soluble MMO component B Gene Probe, although M. capsulatus Baath also produces a soluble MMO.
David M Collins - One of the best experts on this subject based on the ideXlab platform.
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molecular identification of rrna group 3 bacilli ash farrow wallbanks and collins using a pcr Probe test
Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology, 1994Co-Authors: Carol Ash, Fergus G Priest, David M CollinsAbstract:Comparative 16S rRNA sequence analysis has demonstrated that the genusBacillus consists of at least five phyletic lines. rRNA group 3 bacilli of Ash, Farrow, Wallbanks and Collins (1991) comprisingBacillus polymyxa and close relatives is phyloGenetically so removed fromBacillus subtilis, the type species of the genus and other aerobic, endospore-forming bacilli that they warrant reclassification in a new genusPaenibacillus. The genusPaenibacillus can be readily distinguished from otherBacillus groups using a battery of phenotypic characters and a highly specific Gene Probe based on 16S rRNA.
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molecular identification of rrna group 3 bacilli ash farrow wallbanks and collins using a pcr Probe test proposal for the creation of a new genus paenibacillus
Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology, 1994Co-Authors: Carol Ash, Fergus G Priest, David M CollinsAbstract:Comparative 16S rRNA sequence analysis has demonstrated that the genusBacillus consists of at least five phyletic lines. rRNA group 3 bacilli of Ash, Farrow, Wallbanks and Collins (1991) comprisingBacillus polymyxa and close relatives is phyloGenetically so removed fromBacillus subtilis, the type species of the genus and other aerobic, endospore-forming bacilli that they warrant reclassification in a new genusPaenibacillus. The genusPaenibacillus can be readily distinguished from otherBacillus groups using a battery of phenotypic characters and a highly specific Gene Probe based on 16S rRNA.
Milton A Typas - One of the best experts on this subject based on the ideXlab platform.
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restriction fragment length polymorphisms in mitochondrial dna and ribosomal rna Gene complexes as an aid to the characterization of species and sub species populations in the genus verticillium
Fems Microbiology Letters, 1992Co-Authors: Milton A Typas, Alison M Griffen, Brian W Bainbridge, James B HealeAbstract:Genomic DNA was extracted from seven species of Verticillium and digested with the restriction endonucleases EcoRI or HaeIII. Hybridization with an homologous V. albo-atrum ribosomal RNA Gene Probe revealed restriction fragment length polymorphisms (RFLPs) which could differentiate V. lateritium, V. lecanii, V. nigrescens, V. nubilum and V. tricorpus. Digestion with EcoRI did not provide RFLPs which could distinguish between V. albo-atrum and V. dahliae. Digestion of genomic and mitochondrial DNA with HaeIII showed distinctive patterns on ethidium bromide gels which allowed each species to be distinguished. Some intra-species variation in patterns occurred and a combination of mitochondrial and ribosomal RNA Gene complex RFLPs has potential as an aid for the characterization of species and sub-species populations in the Genes Verticillium.