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Goran Roos - One of the best experts on this subject based on the ideXlab platform.
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immunoglobulin heavy chain Gene Rearrangement in peripheral blood mononuclear cells in non hodgkin s lymphomas correlation with kappa lambda analysis and clinical features
European Journal of Haematology, 2009Co-Authors: M Jack D Lindh, Anita Lindstrom, Per Lenner, Erik Lundgren, Goran RoosAbstract:41 patients with non-Hodgkin's lymphomas were analysed to determine occurrence of B-cell monoclonality in peripheral blood mononuclear cells using two different methods: determination of kappa:lambda ratio by light microscopic immunofluorescence, and heavy-chain Gene Rearrangement by DNA-technique. In 21 patients (51%) clonal heavy-chain Rearrangement was found in blood, whilst 18 of the patients (44%) showed and abnormal kappa:lambda ratio. Discordant results between the methods were observed in 5 cases. Clones with Gene Rearrangements suggesting blood involvement were found in 16/25 (64%) patients with low grade lymphomas, in 5/16 (31%) patients with high grade lymphoma, in 17/21 (81%) patients with bone marrow involvement, in 20/27 (74%) of stage III-IV lymphomas and in all of the 14 patients with a high lymphocyte count (greater than or equal to 5.0 X 10(9]. The conclusion was that clonal analysis by the DNA-technique is a more sensitive method than the kappa:lambda determination using immunofluorescence. Even though the method is time-consuming, it could prove to be valuable in selected cases.
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immunoglobulin heavy chain Gene Rearrangement in peripheral blood mononuclear cells in non hodgkin s lymphomas correlation with kappa lambda analysis and clinical features
European Journal of Haematology, 2009Co-Authors: M Jack D Lindh, Anita Lindstrom, Per Lenner, Erik Lundgren, Goran RoosAbstract:: 41 patients with non-Hodgkin's lymphomas were analysed to determine occurrence of B-cell monoclonality in peripheral blood mononuclear cells using two different methods: determination of kappa:lambda ratio by light microscopic immunofluorescence, and heavy-chain Gene Rearrangement by DNA-technique. In 21 patients (51%) clonal heavy-chain Rearrangement was found in blood, whilst 18 of the patients (44%) showed and abnormal kappa:lambda ratio. Discordant results between the methods were observed in 5 cases. Clones with Gene Rearrangements suggesting blood involvement were found in 16/25 (64%) patients with low grade lymphomas, in 5/16 (31%) patients with high grade lymphoma, in 17/21 (81%) patients with bone marrow involvement, in 20/27 (74%) of stage III-IV lymphomas and in all of the 14 patients with a high lymphocyte count (greater than or equal to 5.0 X 10(9]. The conclusion was that clonal analysis by the DNA-technique is a more sensitive method than the kappa:lambda determination using immunofluorescence. Even though the method is time-consuming, it could prove to be valuable in selected cases.
Chichao Chan - One of the best experts on this subject based on the ideXlab platform.
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microdissection and Gene Rearrangement analysis of paraffin embedded specimens of orbital malignant lymphoma
Japanese Journal of Ophthalmology, 2004Co-Authors: Masaru Miyanaga, Chichao Chan, Defen Shen, Motohiro Kiyosawa, Hiroshi Takase, Yoshinobu Eishi, Manabu MochizukiAbstract:To determine whether a definite diagnosis of malignant lymphoma can be made from paraffin-embedded archived orbital specimens by Gene Rearrangement analysis using microdissection and polymerase chain reaction (PCR).
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primary intraocular lymphoma with a low interleukin 10 to interleukin 6 ratio and heteroGeneous igh Gene Rearrangement
Archives of Ophthalmology, 1999Co-Authors: Ronald Buggage, Gisela Velez, Brenda Myerspowell, Scott M Whitcup, Defen Shen, Chichao ChanAbstract:Primary intraocular lymphoma is almost always a central nervous system B-cell non–Hodgkin lymphoma. Primary intraocular lymphoma is commonly diagnosed by demonstrating lymphoma cells in the vitreous or cerebrospinal fluid. An interleukin (IL) 10 to IL-6 ratio greater than 1.0 in these fluids and the detection of immunoglobulin Gene Rearrangement are useful adjuncts in the diagnosis of primary intraocular lymphoma. We report a case of primary intraocular lymphoma diagnosed by chorioretinal biopsy in which no malignant cells were identified in the vitreous and in which the IL-10 to IL-6 ratio was less than 1.0. The detection of IgH Gene Rearrangement heteroGeneity in the tumor cells by polymerase chain reaction, a high tumor mitotic figure rate, and the rapid onset of multiple brain lesions suggest an aggressive malignant neoplasm.
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utility of microdissection and polymerase chain reaction for the detection of immunoglobulin Gene Rearrangement and translocation in primary intraocular lymphoma
Ophthalmology, 1998Co-Authors: Defen Shen, Zhengping Zhuang, P Lehoang, Roland Boni, Sherman Zheng, Robert B Nussenblatt, Chichao ChanAbstract:Abstract Objective Primary intraocular lymphoma, a non-Hodgkin's lymphoma, is a primary central nervous system lymphoma (PCNSL). Diagnosis is usually made by identifying malignant, large B lymphocytes in the vitreous, eye, brain, and cerebral spinal fluid; however, these cells are few, friable, and difficult to recognize. Recently, clonal heavy chain immunoglobulin (IgH) Gene Rearrangement and bcl-2 Gene translocation have been reported in systemic B-cell lymphoma and are used for the detection of malignant cells and in making a diagnosis. The authors investigated the molecular changes in three eyes and a chorioretinal biopsy specimen of four patients with PCNSL. Design Human tissue study. Materials Five ocular specimens of PCNSL were collected. Intervention The first patient had a diagnostic enucleation of the left eye. The second patient underwent diagnostic chorioretinal biopsy. In the third case, a pair of autopsied eyes with reactive lymphoplasmacytic infiltrates of a patient with acquired immune deficiency syndrome (AIDS) were studied. In the fourth case, an enucleated eye of a patient with AIDS-associated lymphoma was sampled. Main outcome measures The bcl-2 and IgH Genes of the lymphoma cells from routine, paraffin-embedded, formaldehyde-fixed, or frozen histologic tissue sections were analyzed using microdissection and polymerase chain reaction (PCR) technique. Results Lymphoma cells obtained from the above four cases showed IgH Rearrangement Gene in the third framework of the V H region. Bcl-2-associated translocation also was detected in three cases (cases 1, 2, and 4). Conclusion Rearrangement of the IgH Gene can serve as a molecular marker for PCNSL. Microdissection allows for procurement and analysis of specific, selected, minute cell populations that are obtained from histologic sections of the complex, heteroGeneous tissue. Translocation of IgH and bcl-2, the apoptotic "survival" signal and proto-oncoGene, could contribute to the pathoGenesis of PCNSL. The combination of microdissection and PCR is a powerful tool for studies of small lesions and cell populations and for understanding disease mechanisms.
Claudeagnes Reynaud - One of the best experts on this subject based on the ideXlab platform.
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immunoglobulin κ light chain Gene Rearrangement is impaired in mice deficient for dna polymerase mu
Immunity, 2003Co-Authors: Barbara Bertocci, Jeanclaude Weill, Annie De Smet, Claudia Berek, Claudeagnes ReynaudAbstract:Abstract DNA polymerase mu (pol μ) is a template-dependent polymerase closely related to the lymphoid-specific enzyme terminal deoxynucleotidyl transferase (TdT). We report here the phenotype of pol μ-deficient mice. Such animals display an abnormal B cell differentiation, with a specific alteration in the IgM − to IgM + transition in bone marrow. In all mice, Ig light chain Gene Rearrangement is impaired at the level of the Vκ-Jκ and Vλ-Jλ junctions, which show extensive nibbling of both coding extremities. These alterations lead to a profound defect in the peripheral B cell compartment which, although variable between animals, results in an average 40% reduction in the splenic B cell fraction. Pol μ appears, therefore, as a key element contributing to the relative homoGeneity in size of light chain CDR3 and taking part in Ig Gene Rearrangement at a stage where TdT is no longer expressed.
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formation of the chicken b cell repertoire ontoGenesis regulation of ig Gene Rearrangement and diversification by Gene conversion
Advances in Immunology, 1994Co-Authors: Claudeagnes Reynaud, Barbara Bertocci, Auriel Dahan, Jeanclaude WeillAbstract:Publisher Summary The chapter focuses on the actual knowledge of the formation of the B-cell repertoire, including data on early B-cell commitment and the regulation of Rearrangement obtained with chicken substrates in transgenic mice. The chicken B-cell immune system is attractive because of its apparent simplicity. During B-cell expansion in the bursa, Gene conversion Generates a diversified B-cell repertoire. Rabbits use Gene conversion to Generate B-cell diversity, and this process may well occur during a short period of development in gut-associated lymphoid tissues (GALT). Sheep, and probably ruminants in General, use gut-associated lymphoid tissues (GALT) that are only present during early development to Generate their B cell repertoire. In chickens, sheep, and rabbits, immunoglobulin (Ig) Gene Rearrangement is not the key event for Ig diversity: The post Rearrangement diversification processes taking place during an early phase of B-cell amplification Generate the B-cell repertoire in these three species. The molecular mechanisms differ, however, among them: in the chicken, Gene conversion diversifies a unique rearranged Gene at both heavy- and light-chain loci by recombination with a pool of pseudo-Gene elements; in the sheep, several functional light-chain V Genes undergo extensive modification by untemplated somatic mutations; and for the rabbit heavy chain, a major rearranged Gene undergoes Gene conversion with possibly extensive somatic mutation of the D region. The organization of Ig Genes in the chicken and the Generation of the chicken B-Cell repertoire by Gene conversion are also discussed in the chapter.
M Jack D Lindh - One of the best experts on this subject based on the ideXlab platform.
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immunoglobulin heavy chain Gene Rearrangement in peripheral blood mononuclear cells in non hodgkin s lymphomas correlation with kappa lambda analysis and clinical features
European Journal of Haematology, 2009Co-Authors: M Jack D Lindh, Anita Lindstrom, Per Lenner, Erik Lundgren, Goran RoosAbstract:41 patients with non-Hodgkin's lymphomas were analysed to determine occurrence of B-cell monoclonality in peripheral blood mononuclear cells using two different methods: determination of kappa:lambda ratio by light microscopic immunofluorescence, and heavy-chain Gene Rearrangement by DNA-technique. In 21 patients (51%) clonal heavy-chain Rearrangement was found in blood, whilst 18 of the patients (44%) showed and abnormal kappa:lambda ratio. Discordant results between the methods were observed in 5 cases. Clones with Gene Rearrangements suggesting blood involvement were found in 16/25 (64%) patients with low grade lymphomas, in 5/16 (31%) patients with high grade lymphoma, in 17/21 (81%) patients with bone marrow involvement, in 20/27 (74%) of stage III-IV lymphomas and in all of the 14 patients with a high lymphocyte count (greater than or equal to 5.0 X 10(9]. The conclusion was that clonal analysis by the DNA-technique is a more sensitive method than the kappa:lambda determination using immunofluorescence. Even though the method is time-consuming, it could prove to be valuable in selected cases.
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immunoglobulin heavy chain Gene Rearrangement in peripheral blood mononuclear cells in non hodgkin s lymphomas correlation with kappa lambda analysis and clinical features
European Journal of Haematology, 2009Co-Authors: M Jack D Lindh, Anita Lindstrom, Per Lenner, Erik Lundgren, Goran RoosAbstract:: 41 patients with non-Hodgkin's lymphomas were analysed to determine occurrence of B-cell monoclonality in peripheral blood mononuclear cells using two different methods: determination of kappa:lambda ratio by light microscopic immunofluorescence, and heavy-chain Gene Rearrangement by DNA-technique. In 21 patients (51%) clonal heavy-chain Rearrangement was found in blood, whilst 18 of the patients (44%) showed and abnormal kappa:lambda ratio. Discordant results between the methods were observed in 5 cases. Clones with Gene Rearrangements suggesting blood involvement were found in 16/25 (64%) patients with low grade lymphomas, in 5/16 (31%) patients with high grade lymphoma, in 17/21 (81%) patients with bone marrow involvement, in 20/27 (74%) of stage III-IV lymphomas and in all of the 14 patients with a high lymphocyte count (greater than or equal to 5.0 X 10(9]. The conclusion was that clonal analysis by the DNA-technique is a more sensitive method than the kappa:lambda determination using immunofluorescence. Even though the method is time-consuming, it could prove to be valuable in selected cases.
Judith Radnay - One of the best experts on this subject based on the ideXlab platform.
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lymphoma with multi Gene Rearrangement on the level of immunoglobulin heavy chain light chains and t cell receptor β chain
American Journal of Hematology, 1997Co-Authors: Ami Klein, Ruth Zemer, Yosef Manor, Hava Shapiro, Mario Cordoba, Isabella Spivak, Judith RadnayAbstract:A unique case with diffuse mixed malignant lymphoma was investigated for Gene Rearrangement on the level of T-cell receptor (TCR), heavy chain immunoglobulin (Ig), and both light chains. Cell phenotype was examined with immunofluorescence techniques using antibodies against surface immunoglobulins (Slg) and the kappa and lambda light chains. Monoclonal antibodies were used against CD3, CD4, CD5, CD8, CD10, CD19, CD22, HLA-DR, and TdT. Gene Rearrangement analysis for monoclonality determination was carried out with restricted DNA (EcoR I and Hind III) hybridized with one of the following 32P-labelled probes: T-cell receptor (TCR |gb), immunoglobulin heavy chain (JH), k light chain, and |gl light chain. Phenotyping of the cell population from the excised lymph node (LN) revealed the presence of 66% B-cells and 35% T-cells. Most of the B cells (94%) expressed μ heavy chain only. Expression of both light chains was negligible (k = 7% and λ = 2%). Gene Rearrangement, which indicates monoclonality, was positive on the level of TCR, Ig heavy chain, and both light chains. The data obtained suggests a neoplastic transforming event in lymphoid stem cells, which preceded the subsequent differentiation process into either B or T lymphoma. Am. J. Hematol. 56:219–223, 1997. © 1997 Wiley-Liss, Inc.