The Experts below are selected from a list of 35487 Experts worldwide ranked by ideXlab platform

Wolfgang Witte - One of the best experts on this subject based on the ideXlab platform.

Guido Werner - One of the best experts on this subject based on the ideXlab platform.

John A Leigh - One of the best experts on this subject based on the ideXlab platform.

  • functional conservation between the argininosuccinate lyase of the archaeon methanococcus maripaludis and the corresponding bacterial and eukaryal genes
    Fems Microbiology Letters, 1999
    Co-Authors: Rachel Cohenkupiec, K Sandbeck, Martin Kupiec, John A Leigh
    Abstract:

    The argH gene encoding argininosuccinate lyase (ASL) of Methanococcus maripaludis was cloned on a 4.7-kb HindIII Genomic Fragment. The gene is preceded by a short open reading frame (ORF149), which encodes a polypeptide with an unknown function. The two genes are co-transcribed. The ASL of M. maripaludis shares a high amino acid identity with ASLs from both bacterial and eukaryal origins and was able to complement both an argH Escherichia coli mutant and an arg4 yeast mutant, showing its extraordinary evolutionary conservation. Attempts to create an argH auxotroph of M. maripaludis by disrupting the Genomic allele were unsuccessful: although a knockout allele of argH was integrated into the M. maripaludis chromosome by homologous recombination, the intact copy was not excluded, suggesting that the argH gene is essential.

  • functional conservation between the argininosuccinate lyase of the archaeon methanococcus maripaludis and the corresponding bacterial and eukaryal genes
    Fems Microbiology Letters, 1999
    Co-Authors: Rachel Cohenkupiec, K Sandbeck, Martin Kupiec, John A Leigh
    Abstract:

    The argH gene encoding argininosuccinate lyase (ASL) of Methanococcus maripaludis was cloned on a 4.7-kb HindIII Genomic Fragment. The gene is preceded by a short open reading frame (ORF149), which encodes a polypeptide with an unknown function. The two genes are co-transcribed. The ASL of M. maripaludis shares a high amino acid identity with ASLs from both bacterial and eukaryal origins and was able to complement both an argH Escherichia coli mutant and an arg4 yeast mutant, showing its extraordinary evolutionary conservation. Attempts to create an argH auxotroph of M. maripaludis by disrupting the Genomic allele were unsuccessful: although a knockout allele of argH was integrated into the M. maripaludis chromosome by homologous recombination, the intact copy was not excluded, suggesting that the argH gene is essential.

William R Jacobs - One of the best experts on this subject based on the ideXlab platform.

  • use of in vivo complementation in mycobacterium tuberculosis to identify a Genomic Fragment associated with virulence
    Infection and Immunity, 1994
    Co-Authors: Lisa Pascopella, F M Collins, J M Martin, Barry R Bloom, Mong Hong Lee, Graham F Hatfull, Charles K Stover, William R Jacobs
    Abstract:

    Novel molecular tools and genetic methods were developed to isolate Genomic Fragments of Mycobacterium tuberculosis that may be associated with virulence. We sought to restore virulence, a characteristic of M. tuberculosis that is correlated with growth rate in mouse spleen and lung tissue, to the avirulent strain H37Ra by complementation. A representative library of the virulent M. tuberculosis strain H37Rv was constructed and transformed into H37Ra. Enrichment for individual faster-growing recombinants was achieved by passage of pools of H37Ra transformants harboring the H37Rv library through mice. A molecular strategy was devised to isolate and clone the H37Rv Genomic DNA Fragment ivg, which conferred a more rapid in vivo growth rate to H37Ra.

  • identification of a Genomic Fragment of mycobacterium tuberculosis responsible for in vivo growth advantage
    Infectious agents and disease, 1993
    Co-Authors: Lisa Pascopella, F M Collins, J M Martin, William R Jacobs, Barry R Bloom
    Abstract:

    The purpose of this work was to develop a system to identify virulence determinants of M. tuberculosis by genetic complementation. The ability to grow in mouse spleen and/or lung was defined as a potential phenotype for virulence. Enrichment for growing recombinant clones from a pool of H37Ra transformants containing the integrating pYUB178::H37Rv cosmid library was accomplished by in vivo selection. A molecular strategy was devised to isolate and clone the 25-kb H37Rv Genomic Fragment ivg that conferred in vivo growth advantage to H37Ra. This study is a first step toward understanding the genetics of virulence in M. tuberculosis. A detailed description of these experiments has been submitted for publication in Infection and Immunity.

Bianca Hildebrandt - One of the best experts on this subject based on the ideXlab platform.