The Experts below are selected from a list of 279 Experts worldwide ranked by ideXlab platform
Mohamed N. Rahaman - One of the best experts on this subject based on the ideXlab platform.
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In vitro bioactivity, cytocompatibility, and antibiotic release profile of Gentamicin Sulfate-loaded borate bioactive glass/chitosan composites
Journal of materials science. Materials in medicine, 2013Co-Authors: Xu Cui, Hui Wang, Zhongping Xie, Shihua Luo, Nai Zhou, Wenhai Huang, Mohamed N. RahamanAbstract:Borate bioactive glass-based composites have been attracting interest recently as an osteoconductive carrier material for local antibiotic delivery. In the present study, composites composed of borate bioactive glass particles bonded with a chitosan matrix were prepared and evaluated in vitro as a carrier for Gentamicin Sulfate. The bioactivity, degradation, drug release profile, and compressive strength of the composite carrier system were studied as a function of immersion time in phosphate-buffered saline at 37 °C. The cytocompatibility of the Gentamicin Sulfate-loaded composite carrier was evaluated using assays of cell proliferation and alkaline phosphatase activity of osteogenic MC3T3-E1 cells. Sustained release of Gentamicin Sulfate occurred over ~28 days in PBS, while the bioactive glass converted continuously to hydroxyapatite. The compressive strength of the composite loaded with Gentamicin Sulfate decreased from the as-fabricated value of 24 ± 3 MPa to ~8 MPa after immersion for 14 days in PBS. Extracts of the soluble ionic products of the borate glass/chitosan composites enhanced the proliferation and alkaline phosphatase activity of MC3T3-E1 cells. These results indicate that the Gentamicin Sulfate-loaded composite composed of chitosan-bonded borate bioactive glass particles could be useful clinically as an osteoconductive carrier material for treating bone infection.
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in vitro bioactivity cytocompatibility and antibiotic release profile of Gentamicin Sulfate loaded borate bioactive glass chitosan composites
Journal of Materials Science: Materials in Medicine, 2013Co-Authors: Xu Cui, Hui Wang, Zhongping Xie, Shihua Luo, Nai Zhou, Wenhai Huang, Mohamed N. RahamanAbstract:Borate bioactive glass-based composites have been attracting interest recently as an osteoconductive carrier material for local antibiotic delivery. In the present study, composites composed of borate bioactive glass particles bonded with a chitosan matrix were prepared and evaluated in vitro as a carrier for Gentamicin Sulfate. The bioactivity, degradation, drug release profile, and compressive strength of the composite carrier system were studied as a function of immersion time in phosphate-buffered saline at 37 °C. The cytocompatibility of the Gentamicin Sulfate-loaded composite carrier was evaluated using assays of cell proliferation and alkaline phosphatase activity of osteogenic MC3T3-E1 cells. Sustained release of Gentamicin Sulfate occurred over ~28 days in PBS, while the bioactive glass converted continuously to hydroxyapatite. The compressive strength of the composite loaded with Gentamicin Sulfate decreased from the as-fabricated value of 24 ± 3 MPa to ~8 MPa after immersion for 14 days in PBS. Extracts of the soluble ionic products of the borate glass/chitosan composites enhanced the proliferation and alkaline phosphatase activity of MC3T3-E1 cells. These results indicate that the Gentamicin Sulfate-loaded composite composed of chitosan-bonded borate bioactive glass particles could be useful clinically as an osteoconductive carrier material for treating bone infection.
R. G. Volz - One of the best experts on this subject based on the ideXlab platform.
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Gentamicin Sulfate attachment and release from anodized ti 6al 4v orthopedic materials
Journal of Biomedical Materials Research, 1993Co-Authors: D. S. Dunn, Srini Raghavan, R. G. VolzAbstract:A novel method has been developed to attach, retain, and release antibiotics from titanium based materials. This technique consists of forming porous surface coatings by anodizing and using the surface chemical properties of the oxide coatings to attach antibiotics. Coatings with pores in the size range 0.1–0.5 μm have been formed in acid solutions. The attachment and retainment of Gentamicin Sulfate, a cationic antibiotic, to the coatings has been investigated using microbiological methods. In vitro test results have shown that the duration of antimicrobial activity on the surface of anodized materials is dependent on the porosity and isoelectric point of the coatings. Using microporous oxide coatings formed in phosphoric acid solutions, it has been found that antimicrobial activity could be retained for more than 2 weeks. © 1993 John Wiley & Sons, Inc.
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Gentamicin Sulfate attachment and release from anodized Ti‐6Al‐4V orthopedic materials
Journal of biomedical materials research, 1993Co-Authors: D. S. Dunn, Srini Raghavan, R. G. VolzAbstract:A novel method has been developed to attach, retain, and release antibiotics from titanium based materials. This technique consists of forming porous surface coatings by anodizing and using the surface chemical properties of the oxide coatings to attach antibiotics. Coatings with pores in the size range 0.1–0.5 μm have been formed in acid solutions. The attachment and retainment of Gentamicin Sulfate, a cationic antibiotic, to the coatings has been investigated using microbiological methods. In vitro test results have shown that the duration of antimicrobial activity on the surface of anodized materials is dependent on the porosity and isoelectric point of the coatings. Using microporous oxide coatings formed in phosphoric acid solutions, it has been found that antimicrobial activity could be retained for more than 2 weeks. © 1993 John Wiley & Sons, Inc.
Xu Cui - One of the best experts on this subject based on the ideXlab platform.
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In vitro bioactivity, cytocompatibility, and antibiotic release profile of Gentamicin Sulfate-loaded borate bioactive glass/chitosan composites
Journal of materials science. Materials in medicine, 2013Co-Authors: Xu Cui, Hui Wang, Zhongping Xie, Shihua Luo, Nai Zhou, Wenhai Huang, Mohamed N. RahamanAbstract:Borate bioactive glass-based composites have been attracting interest recently as an osteoconductive carrier material for local antibiotic delivery. In the present study, composites composed of borate bioactive glass particles bonded with a chitosan matrix were prepared and evaluated in vitro as a carrier for Gentamicin Sulfate. The bioactivity, degradation, drug release profile, and compressive strength of the composite carrier system were studied as a function of immersion time in phosphate-buffered saline at 37 °C. The cytocompatibility of the Gentamicin Sulfate-loaded composite carrier was evaluated using assays of cell proliferation and alkaline phosphatase activity of osteogenic MC3T3-E1 cells. Sustained release of Gentamicin Sulfate occurred over ~28 days in PBS, while the bioactive glass converted continuously to hydroxyapatite. The compressive strength of the composite loaded with Gentamicin Sulfate decreased from the as-fabricated value of 24 ± 3 MPa to ~8 MPa after immersion for 14 days in PBS. Extracts of the soluble ionic products of the borate glass/chitosan composites enhanced the proliferation and alkaline phosphatase activity of MC3T3-E1 cells. These results indicate that the Gentamicin Sulfate-loaded composite composed of chitosan-bonded borate bioactive glass particles could be useful clinically as an osteoconductive carrier material for treating bone infection.
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in vitro bioactivity cytocompatibility and antibiotic release profile of Gentamicin Sulfate loaded borate bioactive glass chitosan composites
Journal of Materials Science: Materials in Medicine, 2013Co-Authors: Xu Cui, Hui Wang, Zhongping Xie, Shihua Luo, Nai Zhou, Wenhai Huang, Mohamed N. RahamanAbstract:Borate bioactive glass-based composites have been attracting interest recently as an osteoconductive carrier material for local antibiotic delivery. In the present study, composites composed of borate bioactive glass particles bonded with a chitosan matrix were prepared and evaluated in vitro as a carrier for Gentamicin Sulfate. The bioactivity, degradation, drug release profile, and compressive strength of the composite carrier system were studied as a function of immersion time in phosphate-buffered saline at 37 °C. The cytocompatibility of the Gentamicin Sulfate-loaded composite carrier was evaluated using assays of cell proliferation and alkaline phosphatase activity of osteogenic MC3T3-E1 cells. Sustained release of Gentamicin Sulfate occurred over ~28 days in PBS, while the bioactive glass converted continuously to hydroxyapatite. The compressive strength of the composite loaded with Gentamicin Sulfate decreased from the as-fabricated value of 24 ± 3 MPa to ~8 MPa after immersion for 14 days in PBS. Extracts of the soluble ionic products of the borate glass/chitosan composites enhanced the proliferation and alkaline phosphatase activity of MC3T3-E1 cells. These results indicate that the Gentamicin Sulfate-loaded composite composed of chitosan-bonded borate bioactive glass particles could be useful clinically as an osteoconductive carrier material for treating bone infection.
D. S. Dunn - One of the best experts on this subject based on the ideXlab platform.
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Gentamicin Sulfate attachment and release from anodized ti 6al 4v orthopedic materials
Journal of Biomedical Materials Research, 1993Co-Authors: D. S. Dunn, Srini Raghavan, R. G. VolzAbstract:A novel method has been developed to attach, retain, and release antibiotics from titanium based materials. This technique consists of forming porous surface coatings by anodizing and using the surface chemical properties of the oxide coatings to attach antibiotics. Coatings with pores in the size range 0.1–0.5 μm have been formed in acid solutions. The attachment and retainment of Gentamicin Sulfate, a cationic antibiotic, to the coatings has been investigated using microbiological methods. In vitro test results have shown that the duration of antimicrobial activity on the surface of anodized materials is dependent on the porosity and isoelectric point of the coatings. Using microporous oxide coatings formed in phosphoric acid solutions, it has been found that antimicrobial activity could be retained for more than 2 weeks. © 1993 John Wiley & Sons, Inc.
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Gentamicin Sulfate attachment and release from anodized Ti‐6Al‐4V orthopedic materials
Journal of biomedical materials research, 1993Co-Authors: D. S. Dunn, Srini Raghavan, R. G. VolzAbstract:A novel method has been developed to attach, retain, and release antibiotics from titanium based materials. This technique consists of forming porous surface coatings by anodizing and using the surface chemical properties of the oxide coatings to attach antibiotics. Coatings with pores in the size range 0.1–0.5 μm have been formed in acid solutions. The attachment and retainment of Gentamicin Sulfate, a cationic antibiotic, to the coatings has been investigated using microbiological methods. In vitro test results have shown that the duration of antimicrobial activity on the surface of anodized materials is dependent on the porosity and isoelectric point of the coatings. Using microporous oxide coatings formed in phosphoric acid solutions, it has been found that antimicrobial activity could be retained for more than 2 weeks. © 1993 John Wiley & Sons, Inc.
Hai Bang Lee - One of the best experts on this subject based on the ideXlab platform.
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Effect of glycolide monomer on release behavior of Gentamicin Sulfate‐loaded PLGA microparticles
Journal of Applied Polymer Science, 2007Co-Authors: Je Young Yoo, Joon Hyun Shin, Gilson Khang, Hyung Sik Shin, Soon Hong Yuk, Yong Sik Kim, Moon Suk Kim, John M. Rhee, Hai Bang LeeAbstract:For the treatment of osteomyelitis and the prevention of infections after orthopedic surgery, topically implantable Gentamicin Sulfate (GS)-loaded poly(D,L-lactide- co-glycolide) microparticles (GSMP) containing glycolide monomer (GM), as a biodegradable and nontoxic material, were prepared by melt-extrusion method without organic solvent for controlled release. After preparation of polymer blend, the powders of different size (90-1200 mm) were obtained by means of freezer-mill. The influences of GM and particle size were investigated on the GS release pat- terns. GSMP containing GM (in case of 10% loaded) showed a near-zero order release from 2 to 7 days with the initial burst. GM affected to increase of GS release rate during the in vitro release test. The pH variations of the media were investigated to determine effect of GM on pH drop of media. The morphological evaluations, change of molecular weight, and thermal property of microparticles were characterized by scanning electron microscope, gel permeation chromatography, and differential scanning cal- orimeter, respectively. Bacterial inhibition zone test was established to identify antibiosis of GS. It showed anti- biotic areas except control sample. From these results, the authors expected that GSMP containing GM would be a good dosage form as a topically implantable device that can get rid of lag period of GSMP. 2007 Wiley Periodicals,
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effect of glycolide monomer on release behavior of Gentamicin Sulfate loaded plga microparticles
Journal of Applied Polymer Science, 2007Co-Authors: Je Young Yoo, Joon Hyun Shin, Gilson Khang, Hyung Sik Shin, Soon Hong Yuk, Yong Sik Kim, Moon Suk Kim, John M. Rhee, Hai Bang LeeAbstract:For the treatment of osteomyelitis and the prevention of infections after orthopedic surgery, topically implantable Gentamicin Sulfate (GS)-loaded poly(D,L-lactide- co-glycolide) microparticles (GSMP) containing glycolide monomer (GM), as a biodegradable and nontoxic material, were prepared by melt-extrusion method without organic solvent for controlled release. After preparation of polymer blend, the powders of different size (90-1200 mm) were obtained by means of freezer-mill. The influences of GM and particle size were investigated on the GS release pat- terns. GSMP containing GM (in case of 10% loaded) showed a near-zero order release from 2 to 7 days with the initial burst. GM affected to increase of GS release rate during the in vitro release test. The pH variations of the media were investigated to determine effect of GM on pH drop of media. The morphological evaluations, change of molecular weight, and thermal property of microparticles were characterized by scanning electron microscope, gel permeation chromatography, and differential scanning cal- orimeter, respectively. Bacterial inhibition zone test was established to identify antibiosis of GS. It showed anti- biotic areas except control sample. From these results, the authors expected that GSMP containing GM would be a good dosage form as a topically implantable device that can get rid of lag period of GSMP. 2007 Wiley Periodicals,
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Effect of lactide/glycolide monomers on release behaviors of Gentamicin Sulfate-loaded PLGA discs.
International journal of pharmaceutics, 2004Co-Authors: Je Young Yoo, Gilson Khang, Moon Suk Kim, Hai Bang Lee, Jong Min Kim, Sun Hang Cho, Yong Sik KimAbstract:In order to develop the desirable drug release patterns such as no lag time and exact zero-order release rate, Gentamicin Sulfate (GS)-loaded poly(D,L-lactide-co-glycolide) (PLGA) discs containing lactide monomer (LM) or glycolide monomer (GM) were prepared. LM or GM was applied for the controlling drug release pattern due to its non-toxic and biodegradable nature. Water absorption, mass loss, pH change, and morphology of discs were examined to study the effect of LM or GM addition. GS release showed near zero-order profile in the GS-loaded polymeric discs prepared in the presence of LM or GM (10%). The channel of GS-loaded PLGA containing LM or GM was formed by the dissolution of LM or GM. Water uptake of disc increased till 21 days from the beginning of the test. The pH variations of media declined in the same manner with the result of mass loss. The antibiosis of GS was also confirmed by bacterial inhibition zone test using the prepared polymeric discs. From these results, we expected that the polymeric discs containing LM or GM would be a good dosage form as a topically implantable device which can get rid of lag period from PLGA matrix.
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effect of lactide glycolide monomers on release behaviors of Gentamicin Sulfate loaded plga discs
International Journal of Pharmaceutics, 2004Co-Authors: Je Young Yoo, Gilson Khang, Moon Suk Kim, Hai Bang Lee, Jong Min Kim, Sun Hang Cho, Yong Sik KimAbstract:In order to develop the desirable drug release patterns such as no lag time and exact zero-order release rate, Gentamicin Sulfate (GS)-loaded poly(D,L-lactide-co-glycolide) (PLGA) discs containing lactide monomer (LM) or glycolide monomer (GM) were prepared. LM or GM was applied for the controlling drug release pattern due to its non-toxic and biodegradable nature. Water absorption, mass loss, pH change, and morphology of discs were examined to study the effect of LM or GM addition. GS release showed near zero-order profile in the GS-loaded polymeric discs prepared in the presence of LM or GM (10%). The channel of GS-loaded PLGA containing LM or GM was formed by the dissolution of LM or GM. Water uptake of disc increased till 21 days from the beginning of the test. The pH variations of media declined in the same manner with the result of mass loss. The antibiosis of GS was also confirmed by bacterial inhibition zone test using the prepared polymeric discs. From these results, we expected that the polymeric discs containing LM or GM would be a good dosage form as a topically implantable device which can get rid of lag period from PLGA matrix.
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controlled release of Gentamicin Sulfate from poly 3 hydroxybutyrate co 3 hydroxyvalerate wafers for the tretment of osteomyelitis
Korea Polymer Journal, 2000Co-Authors: Gilson Khang, John M. Rhee, Jin Cheol Cho, Hak Soo Choi, Sung Chul Yoon, Hai Bang LeeAbstract:Biodegradable wafers were prepared with poly (hydroxybutyrate-co-hydroxyvalerate) (PHBV;5, 10, and 15 mole% for 3-hydroxyvalerate) by simple heat pressing method for the sustained release of antibiotic agent, Gentamicin Sulfate (GS) to investigate the possibility of the treatment for osteomyelitis. The effects of hydroxyvalerate (HV) content, thickness of wafers, various types of additives such as sodium dodecyl Sulfate (SDS), microcrystalline cellulose, polyvinylpyrrolidone, and hydroxypropylcellulose (HPC), and different initial drug loading ratio on the release profile have been investigated. In vitro release studies showed that different release patterns and rates could be achieved by simply modifying factors in the preparation conditions. PHBV wafers with 3 mm thickness, 10% of GS initial loading, 15% of HV content and addition of 5% of SDS and HPC were free from initial burst and a near-zero-order sustained release was observed for over 30 days. It might be suggested that the mechanisms of G5 release may be more predominant simple dissolution and diffusion of GS than erosion of PHBV in our system.