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Tamara N. Nazina - One of the best experts on this subject based on the ideXlab platform.

  • detection of n alkane biodegradation genes alkb and lada in thermophilic hydrocarbon oxidizing bacteria of the genera aeribacillus and Geobacillus
    Microbiology, 2016
    Co-Authors: Tatiana P. Tourova, Sh D Sokolova, E M Semenova, E S Shumkova, Alena V Korshunova, T L Babich, A B Poltaraus, Tamara N. Nazina
    Abstract:

    Ability to degrade crude oil n-alkanes was revealed in new strains of thermophilic bacilli isolated from petroleum reservoirs and a hot spring: Geobacillus toebii В-1024, Geobacillus sp. 1017, and Aeribacillus pallidus 8m3. The strains utilized С10–С30 n-alkanes (В-1024), С10, C11, and С13–С19,22 n-alkanes (1017), and C11–C29 n-alkanes (8m3). In all three strains, PCR amplification with specific degenerate oligonucleotide primers revealed the alkB gene encoding rubredoxin-dependent alkane monooxygenase. In strains В-1024 and 1017, fragments of the genes homologous to the ladA gene determining flavin-dependent alkane monooxygenase were also amplified. Nucleotide sequences of these genes were practically identical to those of the genes ladAαB23, ladAβB23, and ladB B23, which were revealed previously in Geobacillus thermoleovorans strain B23. For the latter strain, activity of respective enzymes in the oxidation of long-chain n-alkanes has been shown. Thus, simultaneous presence of the alkB and ladA genes coding alkane monooxygenases responsible for oxidation of medium-chain and long-chain n-alkanes in thermophilic bacilli was revealed for the first time.

  • characterization of the aerobic hydrocarbon oxidizing enrichments from a high temperature petroleum reservoir by comparative analysis of dna and rna derived clone libraries
    Microbiology, 2011
    Co-Authors: N M Shestakova, Sh D Sokolova, A B Poltaraus, Tatiana P. Tourova, S S Belyaev, A V Korshunova, E M Mikhailova, Tamara N. Nazina
    Abstract:

    Enrichment cultures of aerobic hydrocarbon-oxidizing bacteria obtained from the injection and production wells of the Dagang oil field (China) were studied by molecular biological and microbiological methods. This work is the first to report simultaneous isolation of DNA and RNA from enrichment cultures of microorganisms from oil strata with further construction of clone libraries of 16S rRNA genes and 16S crDNA (complementary rDNA). Comparative analysis of the DNA- and RNA-derived clone libraries made it possible to determine the total genomic diversity of microorganisms, as well as to reveal metabolically active microorganisms in these cultures. Phylotypes of bacteria of the genus Geobacillus were found to be dominant in the DNA and RNA clone libraries of the enrichment cultures from the production well. Phylotypes of bacteria belonging to Geobacillus, Pseudomonas, Tepidiphilus, and other genera were detected in the DNA and RNA libraries obtained from the culture from the injection well. Phylotypes of bacteria of the genus Geobacillus were predominant in the RNA library and represented the second-largest group (after pseudomonads) in the DNA library. In the RNA libraries of the alkB genes of both enrichments, three homologs close to alkB-geo1, alkB-geo2, and alkB-geo4 of bacteria of the genus Geobacillus were detected. The occurrence pattern of the alkB transcripts, ribosomal RNA, and the 16S rRNA genes of bacteria of the genus Geobacillus indicates the predominance and functional activity of geobacilli in the enrichment cultures of hydrocarbon-oxidizing bacteria from high-temperature petroleum reservoir.

  • Geobacillus jurassicus sp nov a new thermophilic bacterium isolated from a high temperature petroleum reservoir and the validation of the Geobacillus species
    Systematic and Applied Microbiology, 2005
    Co-Authors: Tamara N. Nazina, A B Poltaraus, Tatiana P. Tourova, G A Osipov, Alexandre A Grigoryan, Diana Sh Sokolova, Nataliya M Shestakova, Ekaterina M Mikhailova, Anatolii M Lysenko, Sergey S Belyaev
    Abstract:

    Four thermophilic, spore-forming bacterial strains, DS1(T), DS2, 46 and 49, were isolated from the high-temperature Dagang oilfield, located in China. The strains were identified by using the polyphasic taxonomy approach. These were aerobic, gram-positive, rod-shaped, moderately thermophilic (with an optimum growth temperature of 60-65 degrees C), chemoorganotrophic bacteria capable of growing on various sugars, carboxylic acids and crude oil. Two strains, DS1(T) and DS2, were capable of growing on individual saturated hydrocarbons. The G + C content of the DNA of strains DS1(T) and DS2 was 54.5 and 53.8 mol%, respectively. The phylogenetic analysis of the 16S rDNA of strains DS1(T) and DS2 showed that they form a separate cluster within the genus Geobacillus. The cellular fatty acids of the isolates were dominated by iso-15:0, iso-16:0 and iso-17:0 acids, which are the typical fatty acids of bacteria from the genus Geobacillus. The DNA-DNA hybridization study and the comparative analysis of the morphological and chemotaxonomic characteristics of strains DS1(T) and DS2 showed that they differ from the previously described Geobacillus species and belong to a new species, which was called Geobacillus jurassicus. DS1(T) (=VKM B2301(T), = DSM 15726(T)) is the type strain of this species. According to both DNA-DNA reassociation studies and 16S rDNA sequence analysis, two other strains, 46 and 49, were assigned to the species G. stearothermophilus. In this paper, we provide evidence that the new combinations G. stearothermophilus, G. thermoleovorans, G. kaustophilus, G. thermoglucosidasius and G. thermodenitrificans may be considered to be valid.

  • Geobacillus gargensis sp nov a novel thermophile from a hot spring and the reclassification of bacillus vulcani as Geobacillus vulcani comb nov
    International Journal of Systematic and Evolutionary Microbiology, 2004
    Co-Authors: Tamara N. Nazina, A B Poltaraus, Tatiana P. Tourova, Elena V Lebedeva, Alexandre A Grigorya, Diyana S Sokolova, Anatoliy M Lysenko, G A Osipov
    Abstract:

    A novel thermophilic spore-forming strain, GaT, was isolated from the Garga hot spring located in the northern part of the Transbaikal region (Russia). Strain GaT was found to be an aerobic, Gram-positive, rod-shaped, thermophilic (optimum growth temperature is 60–65 °C), chemo-organotrophic bacterium that grows on various sugars, carboxylic acids and hydrocarbons. The G+C content of its DNA is 52·9 mol%. The 16S rRNA gene sequence similarity data show that strain GaT is closely related to members of the genus Geobacillus. Relevant chemotaxonomic data (in particular, the major fatty acid profile of strain GaT, which includes iso-C15 : 0, iso-C16 : 0 and iso-C17 : 0 acids) support the assignment of this strain to the genus Geobacillus. The physiological, biochemical and DNA–DNA hybridization studies of strain GaT showed that it differs both genotypically and phenotypically from the recognized Geobacillus species. Based on these data, strain GaT belongs to a novel species, Geobacillus gargensis sp. nov. (type strain, GaT=VKM B-2300T=DSM 15378T). The analysis of the phenotypic characteristics (additional to those given in the original description) of the type strain of Bacillus vulcani (DSM 13174T) showed that they are very similar to the major phenotypic characteristics of the genus Geobacillus. The low DNA–DNA reassociation values of strain DSM 13174T with various species of this genus (from 38 to 54 %) clearly demonstrate a sufficient genomic distinction of this strain and its taxonomic status as a species. The physiological characteristics, phylogenetic position and DNA–DNA reassociation values of B. vulcani allow this species to be reclassified as Geobacillus vulcani comb. nov. The main properties that differentiate G. vulcani from the other species of the genus are its ability to produce acids from glycerol, lactose and ribose.

  • Geobacillus gargensis sp nov a novel thermophile from a hot spring and the reclassification of bacillus vulcani as Geobacillus vulcani comb nov
    International Journal of Systematic and Evolutionary Microbiology, 2004
    Co-Authors: Tamara N. Nazina, A B Poltaraus, Tatiana P. Tourova, Elena V Lebedeva, Diyana S Sokolova, Anatoliy M Lysenko, Alexandre A Grigoryan, G A Osipov
    Abstract:

    A novel thermophilic spore-forming strain, Ga(T), was isolated from the Garga hot spring located in the northern part of the Transbaikal region (Russia). Strain Ga(T) was found to be an aerobic, Gram-positive, rod-shaped, thermophilic (optimum growth temperature is 60-65 degrees C), chemo-organotrophic bacterium that grows on various sugars, carboxylic acids and hydrocarbons. The G+C content of its DNA is 52.9 mol%. The 16S rRNA gene sequence similarity data show that strain Ga(T) is closely related to members of the genus Geobacillus. Relevant chemotaxonomic data (in particular, the major fatty acid profile of strain Ga(T), which includes iso-C15 : 0, iso-C16 : 0 and iso-C17 : 0 acids) support the assignment of this strain to the genus Geobacillus. The physiological, biochemical and DNA-DNA hybridization studies of strain Ga(T) showed that it differs both genotypically and phenotypically from the recognized Geobacillus species. Based on these data, strain Ga(T) belongs to a novel species, Geobacillus gargensis sp. nov. (type strain, Ga(T)=VKM B-2300(T)=DSM 15378(T)). The analysis of the phenotypic characteristics (additional to those given in the original description) of the type strain of Bacillus vulcani (DSM 13174(T)) showed that they are very similar to the major phenotypic characteristics of the genus Geobacillus. The low DNA-DNA reassociation values of strain DSM 13174(T) with various species of this genus (from 38 to 54 %) clearly demonstrate a sufficient genomic distinction of this strain and its taxonomic status as a species. The physiological characteristics, phylogenetic position and DNA-DNA reassociation values of B. vulcani allow this species to be reclassified as Geobacillus vulcani comb. nov. The main properties that differentiate G. vulcani from the other species of the genus are its ability to produce acids from glycerol, lactose and ribose.

G A Osipov - One of the best experts on this subject based on the ideXlab platform.

  • Geobacillus jurassicus sp nov a new thermophilic bacterium isolated from a high temperature petroleum reservoir and the validation of the Geobacillus species
    Systematic and Applied Microbiology, 2005
    Co-Authors: Tamara N. Nazina, A B Poltaraus, Tatiana P. Tourova, G A Osipov, Alexandre A Grigoryan, Diana Sh Sokolova, Nataliya M Shestakova, Ekaterina M Mikhailova, Anatolii M Lysenko, Sergey S Belyaev
    Abstract:

    Four thermophilic, spore-forming bacterial strains, DS1(T), DS2, 46 and 49, were isolated from the high-temperature Dagang oilfield, located in China. The strains were identified by using the polyphasic taxonomy approach. These were aerobic, gram-positive, rod-shaped, moderately thermophilic (with an optimum growth temperature of 60-65 degrees C), chemoorganotrophic bacteria capable of growing on various sugars, carboxylic acids and crude oil. Two strains, DS1(T) and DS2, were capable of growing on individual saturated hydrocarbons. The G + C content of the DNA of strains DS1(T) and DS2 was 54.5 and 53.8 mol%, respectively. The phylogenetic analysis of the 16S rDNA of strains DS1(T) and DS2 showed that they form a separate cluster within the genus Geobacillus. The cellular fatty acids of the isolates were dominated by iso-15:0, iso-16:0 and iso-17:0 acids, which are the typical fatty acids of bacteria from the genus Geobacillus. The DNA-DNA hybridization study and the comparative analysis of the morphological and chemotaxonomic characteristics of strains DS1(T) and DS2 showed that they differ from the previously described Geobacillus species and belong to a new species, which was called Geobacillus jurassicus. DS1(T) (=VKM B2301(T), = DSM 15726(T)) is the type strain of this species. According to both DNA-DNA reassociation studies and 16S rDNA sequence analysis, two other strains, 46 and 49, were assigned to the species G. stearothermophilus. In this paper, we provide evidence that the new combinations G. stearothermophilus, G. thermoleovorans, G. kaustophilus, G. thermoglucosidasius and G. thermodenitrificans may be considered to be valid.

  • Geobacillus gargensis sp nov a novel thermophile from a hot spring and the reclassification of bacillus vulcani as Geobacillus vulcani comb nov
    International Journal of Systematic and Evolutionary Microbiology, 2004
    Co-Authors: Tamara N. Nazina, A B Poltaraus, Tatiana P. Tourova, Elena V Lebedeva, Alexandre A Grigorya, Diyana S Sokolova, Anatoliy M Lysenko, G A Osipov
    Abstract:

    A novel thermophilic spore-forming strain, GaT, was isolated from the Garga hot spring located in the northern part of the Transbaikal region (Russia). Strain GaT was found to be an aerobic, Gram-positive, rod-shaped, thermophilic (optimum growth temperature is 60–65 °C), chemo-organotrophic bacterium that grows on various sugars, carboxylic acids and hydrocarbons. The G+C content of its DNA is 52·9 mol%. The 16S rRNA gene sequence similarity data show that strain GaT is closely related to members of the genus Geobacillus. Relevant chemotaxonomic data (in particular, the major fatty acid profile of strain GaT, which includes iso-C15 : 0, iso-C16 : 0 and iso-C17 : 0 acids) support the assignment of this strain to the genus Geobacillus. The physiological, biochemical and DNA–DNA hybridization studies of strain GaT showed that it differs both genotypically and phenotypically from the recognized Geobacillus species. Based on these data, strain GaT belongs to a novel species, Geobacillus gargensis sp. nov. (type strain, GaT=VKM B-2300T=DSM 15378T). The analysis of the phenotypic characteristics (additional to those given in the original description) of the type strain of Bacillus vulcani (DSM 13174T) showed that they are very similar to the major phenotypic characteristics of the genus Geobacillus. The low DNA–DNA reassociation values of strain DSM 13174T with various species of this genus (from 38 to 54 %) clearly demonstrate a sufficient genomic distinction of this strain and its taxonomic status as a species. The physiological characteristics, phylogenetic position and DNA–DNA reassociation values of B. vulcani allow this species to be reclassified as Geobacillus vulcani comb. nov. The main properties that differentiate G. vulcani from the other species of the genus are its ability to produce acids from glycerol, lactose and ribose.

  • Geobacillus gargensis sp nov a novel thermophile from a hot spring and the reclassification of bacillus vulcani as Geobacillus vulcani comb nov
    International Journal of Systematic and Evolutionary Microbiology, 2004
    Co-Authors: Tamara N. Nazina, A B Poltaraus, Tatiana P. Tourova, Elena V Lebedeva, Diyana S Sokolova, Anatoliy M Lysenko, Alexandre A Grigoryan, G A Osipov
    Abstract:

    A novel thermophilic spore-forming strain, Ga(T), was isolated from the Garga hot spring located in the northern part of the Transbaikal region (Russia). Strain Ga(T) was found to be an aerobic, Gram-positive, rod-shaped, thermophilic (optimum growth temperature is 60-65 degrees C), chemo-organotrophic bacterium that grows on various sugars, carboxylic acids and hydrocarbons. The G+C content of its DNA is 52.9 mol%. The 16S rRNA gene sequence similarity data show that strain Ga(T) is closely related to members of the genus Geobacillus. Relevant chemotaxonomic data (in particular, the major fatty acid profile of strain Ga(T), which includes iso-C15 : 0, iso-C16 : 0 and iso-C17 : 0 acids) support the assignment of this strain to the genus Geobacillus. The physiological, biochemical and DNA-DNA hybridization studies of strain Ga(T) showed that it differs both genotypically and phenotypically from the recognized Geobacillus species. Based on these data, strain Ga(T) belongs to a novel species, Geobacillus gargensis sp. nov. (type strain, Ga(T)=VKM B-2300(T)=DSM 15378(T)). The analysis of the phenotypic characteristics (additional to those given in the original description) of the type strain of Bacillus vulcani (DSM 13174(T)) showed that they are very similar to the major phenotypic characteristics of the genus Geobacillus. The low DNA-DNA reassociation values of strain DSM 13174(T) with various species of this genus (from 38 to 54 %) clearly demonstrate a sufficient genomic distinction of this strain and its taxonomic status as a species. The physiological characteristics, phylogenetic position and DNA-DNA reassociation values of B. vulcani allow this species to be reclassified as Geobacillus vulcani comb. nov. The main properties that differentiate G. vulcani from the other species of the genus are its ability to produce acids from glycerol, lactose and ribose.

  • taxonomic study of aerobic thermophilic bacilli descriptions of Geobacillus subterraneus gen nov sp nov and Geobacillus uzenensis sp nov from petroleum reservoirs and transfer of bacillus stearothermophilus bacillus thermocatenulatus bacillus thermoleovorans bacillus kaustophilus bacillus thermodenitrificans to Geobacillus as the new combinations g stearothermophilus g th
    International Journal of Systematic and Evolutionary Microbiology, 2001
    Co-Authors: Tamara N. Nazina, A B Poltaraus, Tatiana P. Tourova, G A Osipov, E V Novikova, A A Grigorya, A E Ivanova, A M Lysenko, V V Petrunyaka, S S Elyaev
    Abstract:

    Five hydrocarbon-oxidizing strains were isolated from formation waters of oilfields in Russia, Kazakhstan and China. These strains were moderately thermophilic, neutrophilic, motile, spore-forming rods, aerobic or facultatively anaerobic. The G+C content of their DNA ranged from 49.7 to 52.3 mol%. The major isoprenoid quinone was menaquinone-7; cellular fatty acid profiles consisted of significant amounts of iso-15:0, iso-16:0 and iso-17:0 fatty acids (61.7-86.8% of the total). Based on data from 16S rDNA analysis and DNA-DNA hybridization, the subsurface isolates could be divided into two groups, one of which consisted of strains UT and X and the other of which consisted of strains K, Sam and 34T. The new strains exhibited a close phylogenetic relationship to thermophilic bacilli of 'Group 5' of Ash et al. [Ash, C., Farrow, J. A. E., Wallbanks, S. & Collins, M. D. (1991). Lett Appl Microbiol 13, 202-206] and a set of corresponding signature positions of 16S rRNA. Comparative analysis of the 16S rDNA sequences and fatty acid compositions of the novel isolates and established species of thermophilic bacilli indicated that the subsurface strains represent two new species within a new genus, for which the names Geobacillus subterraneus gen. nov., sp. nov., and Geobacillus uzenensis sp. nov. are proposed. It is also proposed that Bacillus stearothermophilus, Bacillus thermoleovorans, Bacillus thermocatenulatus, Bacillus kaustophilus, Bacillus thermoglucosidasius and Bacillus thermodenitrificans be transferred to this new genus, with Geobacillus stearothermophilus (formerly Bacillus stearothermophilus) as the type species.

Nitu Sood - One of the best experts on this subject based on the ideXlab platform.

  • isolation and characterization of a potential paraffin wax degrading thermophilic bacterial strain Geobacillus kaustophilus teri nsm for application in oil wells with paraffin deposition problems
    Chemosphere, 2008
    Co-Authors: Nitu Sood
    Abstract:

    Abstract Paraffin deposition problems, that have plagued the oil industry, are currently remediated by mechanical and chemical means. However, since these methods are problematic, a microbiological approach has been considered. The bacteria, required for the mitigation of paraffin deposition problems, should be able to survive the high temperatures of oil wells and degrade the paraffins under low oxygen and nutrient conditions while sparing the low carbon chain paraffins. In this study, a thermophilic paraffinic wax degrading bacterial strain was isolated from a soil sample contaminated with paraffinic crude oil. The selected strain, Geobacillus TERI NSM , could degrade 600 mg of paraffinic wax as the sole carbon source in 1000 ml minimal salts medium in 7 d at 55 °C. This strain was identified as Geobacillus kaustophilus by fatty acid methyl esters analysis and 16S rRNA full gene sequencing. G. kaustophilus TERI NSM showed 97% degradation of eicosane, 85% degradation of pentacosane and 77% degradation of triacontane in 10 d when used as the carbon source. The strain TERI NSM could also degrade the paraffins of crude oil collected from oil wells that had a history of paraffin deposition problems.

  • isolation and characterization of a potential paraffin wax degrading thermophilic bacterial strain Geobacillus kaustophilus teri nsm for application in oil wells with paraffin deposition problems
    Chemosphere, 2008
    Co-Authors: Nitu Sood, Banwari Lal
    Abstract:

    Paraffin deposition problems, that have plagued the oil industry, are currently remediated by mechanical and chemical means. However, since these methods are problematic, a microbiological approach has been considered. The bacteria, required for the mitigation of paraffin deposition problems, should be able to survive the high temperatures of oil wells and degrade the paraffins under low oxygen and nutrient conditions while sparing the low carbon chain paraffins. In this study, a thermophilic paraffinic wax degrading bacterial strain was isolated from a soil sample contaminated with paraffinic crude oil. The selected strain, Geobacillus TERI NSM, could degrade 600mg of paraffinic wax as the sole carbon source in 1000ml minimal salts medium in 7d at 55 degrees C. This strain was identified as Geobacillus kaustophilus by fatty acid methyl esters analysis and 16S rRNA full gene sequencing. G. kaustophilus TERI NSM showed 97% degradation of eicosane, 85% degradation of pentacosane and 77% degradation of triacontane in 10d when used as the carbon source. The strain TERI NSM could also degrade the paraffins of crude oil collected from oil wells that had a history of paraffin deposition problems.

A B Poltaraus - One of the best experts on this subject based on the ideXlab platform.

  • detection of n alkane biodegradation genes alkb and lada in thermophilic hydrocarbon oxidizing bacteria of the genera aeribacillus and Geobacillus
    Microbiology, 2016
    Co-Authors: Tatiana P. Tourova, Sh D Sokolova, E M Semenova, E S Shumkova, Alena V Korshunova, T L Babich, A B Poltaraus, Tamara N. Nazina
    Abstract:

    Ability to degrade crude oil n-alkanes was revealed in new strains of thermophilic bacilli isolated from petroleum reservoirs and a hot spring: Geobacillus toebii В-1024, Geobacillus sp. 1017, and Aeribacillus pallidus 8m3. The strains utilized С10–С30 n-alkanes (В-1024), С10, C11, and С13–С19,22 n-alkanes (1017), and C11–C29 n-alkanes (8m3). In all three strains, PCR amplification with specific degenerate oligonucleotide primers revealed the alkB gene encoding rubredoxin-dependent alkane monooxygenase. In strains В-1024 and 1017, fragments of the genes homologous to the ladA gene determining flavin-dependent alkane monooxygenase were also amplified. Nucleotide sequences of these genes were practically identical to those of the genes ladAαB23, ladAβB23, and ladB B23, which were revealed previously in Geobacillus thermoleovorans strain B23. For the latter strain, activity of respective enzymes in the oxidation of long-chain n-alkanes has been shown. Thus, simultaneous presence of the alkB and ladA genes coding alkane monooxygenases responsible for oxidation of medium-chain and long-chain n-alkanes in thermophilic bacilli was revealed for the first time.

  • characterization of the aerobic hydrocarbon oxidizing enrichments from a high temperature petroleum reservoir by comparative analysis of dna and rna derived clone libraries
    Microbiology, 2011
    Co-Authors: N M Shestakova, Sh D Sokolova, A B Poltaraus, Tatiana P. Tourova, S S Belyaev, A V Korshunova, E M Mikhailova, Tamara N. Nazina
    Abstract:

    Enrichment cultures of aerobic hydrocarbon-oxidizing bacteria obtained from the injection and production wells of the Dagang oil field (China) were studied by molecular biological and microbiological methods. This work is the first to report simultaneous isolation of DNA and RNA from enrichment cultures of microorganisms from oil strata with further construction of clone libraries of 16S rRNA genes and 16S crDNA (complementary rDNA). Comparative analysis of the DNA- and RNA-derived clone libraries made it possible to determine the total genomic diversity of microorganisms, as well as to reveal metabolically active microorganisms in these cultures. Phylotypes of bacteria of the genus Geobacillus were found to be dominant in the DNA and RNA clone libraries of the enrichment cultures from the production well. Phylotypes of bacteria belonging to Geobacillus, Pseudomonas, Tepidiphilus, and other genera were detected in the DNA and RNA libraries obtained from the culture from the injection well. Phylotypes of bacteria of the genus Geobacillus were predominant in the RNA library and represented the second-largest group (after pseudomonads) in the DNA library. In the RNA libraries of the alkB genes of both enrichments, three homologs close to alkB-geo1, alkB-geo2, and alkB-geo4 of bacteria of the genus Geobacillus were detected. The occurrence pattern of the alkB transcripts, ribosomal RNA, and the 16S rRNA genes of bacteria of the genus Geobacillus indicates the predominance and functional activity of geobacilli in the enrichment cultures of hydrocarbon-oxidizing bacteria from high-temperature petroleum reservoir.

  • Geobacillus jurassicus sp nov a new thermophilic bacterium isolated from a high temperature petroleum reservoir and the validation of the Geobacillus species
    Systematic and Applied Microbiology, 2005
    Co-Authors: Tamara N. Nazina, A B Poltaraus, Tatiana P. Tourova, G A Osipov, Alexandre A Grigoryan, Diana Sh Sokolova, Nataliya M Shestakova, Ekaterina M Mikhailova, Anatolii M Lysenko, Sergey S Belyaev
    Abstract:

    Four thermophilic, spore-forming bacterial strains, DS1(T), DS2, 46 and 49, were isolated from the high-temperature Dagang oilfield, located in China. The strains were identified by using the polyphasic taxonomy approach. These were aerobic, gram-positive, rod-shaped, moderately thermophilic (with an optimum growth temperature of 60-65 degrees C), chemoorganotrophic bacteria capable of growing on various sugars, carboxylic acids and crude oil. Two strains, DS1(T) and DS2, were capable of growing on individual saturated hydrocarbons. The G + C content of the DNA of strains DS1(T) and DS2 was 54.5 and 53.8 mol%, respectively. The phylogenetic analysis of the 16S rDNA of strains DS1(T) and DS2 showed that they form a separate cluster within the genus Geobacillus. The cellular fatty acids of the isolates were dominated by iso-15:0, iso-16:0 and iso-17:0 acids, which are the typical fatty acids of bacteria from the genus Geobacillus. The DNA-DNA hybridization study and the comparative analysis of the morphological and chemotaxonomic characteristics of strains DS1(T) and DS2 showed that they differ from the previously described Geobacillus species and belong to a new species, which was called Geobacillus jurassicus. DS1(T) (=VKM B2301(T), = DSM 15726(T)) is the type strain of this species. According to both DNA-DNA reassociation studies and 16S rDNA sequence analysis, two other strains, 46 and 49, were assigned to the species G. stearothermophilus. In this paper, we provide evidence that the new combinations G. stearothermophilus, G. thermoleovorans, G. kaustophilus, G. thermoglucosidasius and G. thermodenitrificans may be considered to be valid.

  • Geobacillus gargensis sp nov a novel thermophile from a hot spring and the reclassification of bacillus vulcani as Geobacillus vulcani comb nov
    International Journal of Systematic and Evolutionary Microbiology, 2004
    Co-Authors: Tamara N. Nazina, A B Poltaraus, Tatiana P. Tourova, Elena V Lebedeva, Alexandre A Grigorya, Diyana S Sokolova, Anatoliy M Lysenko, G A Osipov
    Abstract:

    A novel thermophilic spore-forming strain, GaT, was isolated from the Garga hot spring located in the northern part of the Transbaikal region (Russia). Strain GaT was found to be an aerobic, Gram-positive, rod-shaped, thermophilic (optimum growth temperature is 60–65 °C), chemo-organotrophic bacterium that grows on various sugars, carboxylic acids and hydrocarbons. The G+C content of its DNA is 52·9 mol%. The 16S rRNA gene sequence similarity data show that strain GaT is closely related to members of the genus Geobacillus. Relevant chemotaxonomic data (in particular, the major fatty acid profile of strain GaT, which includes iso-C15 : 0, iso-C16 : 0 and iso-C17 : 0 acids) support the assignment of this strain to the genus Geobacillus. The physiological, biochemical and DNA–DNA hybridization studies of strain GaT showed that it differs both genotypically and phenotypically from the recognized Geobacillus species. Based on these data, strain GaT belongs to a novel species, Geobacillus gargensis sp. nov. (type strain, GaT=VKM B-2300T=DSM 15378T). The analysis of the phenotypic characteristics (additional to those given in the original description) of the type strain of Bacillus vulcani (DSM 13174T) showed that they are very similar to the major phenotypic characteristics of the genus Geobacillus. The low DNA–DNA reassociation values of strain DSM 13174T with various species of this genus (from 38 to 54 %) clearly demonstrate a sufficient genomic distinction of this strain and its taxonomic status as a species. The physiological characteristics, phylogenetic position and DNA–DNA reassociation values of B. vulcani allow this species to be reclassified as Geobacillus vulcani comb. nov. The main properties that differentiate G. vulcani from the other species of the genus are its ability to produce acids from glycerol, lactose and ribose.

  • Geobacillus gargensis sp nov a novel thermophile from a hot spring and the reclassification of bacillus vulcani as Geobacillus vulcani comb nov
    International Journal of Systematic and Evolutionary Microbiology, 2004
    Co-Authors: Tamara N. Nazina, A B Poltaraus, Tatiana P. Tourova, Elena V Lebedeva, Diyana S Sokolova, Anatoliy M Lysenko, Alexandre A Grigoryan, G A Osipov
    Abstract:

    A novel thermophilic spore-forming strain, Ga(T), was isolated from the Garga hot spring located in the northern part of the Transbaikal region (Russia). Strain Ga(T) was found to be an aerobic, Gram-positive, rod-shaped, thermophilic (optimum growth temperature is 60-65 degrees C), chemo-organotrophic bacterium that grows on various sugars, carboxylic acids and hydrocarbons. The G+C content of its DNA is 52.9 mol%. The 16S rRNA gene sequence similarity data show that strain Ga(T) is closely related to members of the genus Geobacillus. Relevant chemotaxonomic data (in particular, the major fatty acid profile of strain Ga(T), which includes iso-C15 : 0, iso-C16 : 0 and iso-C17 : 0 acids) support the assignment of this strain to the genus Geobacillus. The physiological, biochemical and DNA-DNA hybridization studies of strain Ga(T) showed that it differs both genotypically and phenotypically from the recognized Geobacillus species. Based on these data, strain Ga(T) belongs to a novel species, Geobacillus gargensis sp. nov. (type strain, Ga(T)=VKM B-2300(T)=DSM 15378(T)). The analysis of the phenotypic characteristics (additional to those given in the original description) of the type strain of Bacillus vulcani (DSM 13174(T)) showed that they are very similar to the major phenotypic characteristics of the genus Geobacillus. The low DNA-DNA reassociation values of strain DSM 13174(T) with various species of this genus (from 38 to 54 %) clearly demonstrate a sufficient genomic distinction of this strain and its taxonomic status as a species. The physiological characteristics, phylogenetic position and DNA-DNA reassociation values of B. vulcani allow this species to be reclassified as Geobacillus vulcani comb. nov. The main properties that differentiate G. vulcani from the other species of the genus are its ability to produce acids from glycerol, lactose and ribose.

Tatiana P. Tourova - One of the best experts on this subject based on the ideXlab platform.

  • detection of n alkane biodegradation genes alkb and lada in thermophilic hydrocarbon oxidizing bacteria of the genera aeribacillus and Geobacillus
    Microbiology, 2016
    Co-Authors: Tatiana P. Tourova, Sh D Sokolova, E M Semenova, E S Shumkova, Alena V Korshunova, T L Babich, A B Poltaraus, Tamara N. Nazina
    Abstract:

    Ability to degrade crude oil n-alkanes was revealed in new strains of thermophilic bacilli isolated from petroleum reservoirs and a hot spring: Geobacillus toebii В-1024, Geobacillus sp. 1017, and Aeribacillus pallidus 8m3. The strains utilized С10–С30 n-alkanes (В-1024), С10, C11, and С13–С19,22 n-alkanes (1017), and C11–C29 n-alkanes (8m3). In all three strains, PCR amplification with specific degenerate oligonucleotide primers revealed the alkB gene encoding rubredoxin-dependent alkane monooxygenase. In strains В-1024 and 1017, fragments of the genes homologous to the ladA gene determining flavin-dependent alkane monooxygenase were also amplified. Nucleotide sequences of these genes were practically identical to those of the genes ladAαB23, ladAβB23, and ladB B23, which were revealed previously in Geobacillus thermoleovorans strain B23. For the latter strain, activity of respective enzymes in the oxidation of long-chain n-alkanes has been shown. Thus, simultaneous presence of the alkB and ladA genes coding alkane monooxygenases responsible for oxidation of medium-chain and long-chain n-alkanes in thermophilic bacilli was revealed for the first time.

  • characterization of the aerobic hydrocarbon oxidizing enrichments from a high temperature petroleum reservoir by comparative analysis of dna and rna derived clone libraries
    Microbiology, 2011
    Co-Authors: N M Shestakova, Sh D Sokolova, A B Poltaraus, Tatiana P. Tourova, S S Belyaev, A V Korshunova, E M Mikhailova, Tamara N. Nazina
    Abstract:

    Enrichment cultures of aerobic hydrocarbon-oxidizing bacteria obtained from the injection and production wells of the Dagang oil field (China) were studied by molecular biological and microbiological methods. This work is the first to report simultaneous isolation of DNA and RNA from enrichment cultures of microorganisms from oil strata with further construction of clone libraries of 16S rRNA genes and 16S crDNA (complementary rDNA). Comparative analysis of the DNA- and RNA-derived clone libraries made it possible to determine the total genomic diversity of microorganisms, as well as to reveal metabolically active microorganisms in these cultures. Phylotypes of bacteria of the genus Geobacillus were found to be dominant in the DNA and RNA clone libraries of the enrichment cultures from the production well. Phylotypes of bacteria belonging to Geobacillus, Pseudomonas, Tepidiphilus, and other genera were detected in the DNA and RNA libraries obtained from the culture from the injection well. Phylotypes of bacteria of the genus Geobacillus were predominant in the RNA library and represented the second-largest group (after pseudomonads) in the DNA library. In the RNA libraries of the alkB genes of both enrichments, three homologs close to alkB-geo1, alkB-geo2, and alkB-geo4 of bacteria of the genus Geobacillus were detected. The occurrence pattern of the alkB transcripts, ribosomal RNA, and the 16S rRNA genes of bacteria of the genus Geobacillus indicates the predominance and functional activity of geobacilli in the enrichment cultures of hydrocarbon-oxidizing bacteria from high-temperature petroleum reservoir.

  • Geobacillus jurassicus sp nov a new thermophilic bacterium isolated from a high temperature petroleum reservoir and the validation of the Geobacillus species
    Systematic and Applied Microbiology, 2005
    Co-Authors: Tamara N. Nazina, A B Poltaraus, Tatiana P. Tourova, G A Osipov, Alexandre A Grigoryan, Diana Sh Sokolova, Nataliya M Shestakova, Ekaterina M Mikhailova, Anatolii M Lysenko, Sergey S Belyaev
    Abstract:

    Four thermophilic, spore-forming bacterial strains, DS1(T), DS2, 46 and 49, were isolated from the high-temperature Dagang oilfield, located in China. The strains were identified by using the polyphasic taxonomy approach. These were aerobic, gram-positive, rod-shaped, moderately thermophilic (with an optimum growth temperature of 60-65 degrees C), chemoorganotrophic bacteria capable of growing on various sugars, carboxylic acids and crude oil. Two strains, DS1(T) and DS2, were capable of growing on individual saturated hydrocarbons. The G + C content of the DNA of strains DS1(T) and DS2 was 54.5 and 53.8 mol%, respectively. The phylogenetic analysis of the 16S rDNA of strains DS1(T) and DS2 showed that they form a separate cluster within the genus Geobacillus. The cellular fatty acids of the isolates were dominated by iso-15:0, iso-16:0 and iso-17:0 acids, which are the typical fatty acids of bacteria from the genus Geobacillus. The DNA-DNA hybridization study and the comparative analysis of the morphological and chemotaxonomic characteristics of strains DS1(T) and DS2 showed that they differ from the previously described Geobacillus species and belong to a new species, which was called Geobacillus jurassicus. DS1(T) (=VKM B2301(T), = DSM 15726(T)) is the type strain of this species. According to both DNA-DNA reassociation studies and 16S rDNA sequence analysis, two other strains, 46 and 49, were assigned to the species G. stearothermophilus. In this paper, we provide evidence that the new combinations G. stearothermophilus, G. thermoleovorans, G. kaustophilus, G. thermoglucosidasius and G. thermodenitrificans may be considered to be valid.

  • Geobacillus gargensis sp nov a novel thermophile from a hot spring and the reclassification of bacillus vulcani as Geobacillus vulcani comb nov
    International Journal of Systematic and Evolutionary Microbiology, 2004
    Co-Authors: Tamara N. Nazina, A B Poltaraus, Tatiana P. Tourova, Elena V Lebedeva, Alexandre A Grigorya, Diyana S Sokolova, Anatoliy M Lysenko, G A Osipov
    Abstract:

    A novel thermophilic spore-forming strain, GaT, was isolated from the Garga hot spring located in the northern part of the Transbaikal region (Russia). Strain GaT was found to be an aerobic, Gram-positive, rod-shaped, thermophilic (optimum growth temperature is 60–65 °C), chemo-organotrophic bacterium that grows on various sugars, carboxylic acids and hydrocarbons. The G+C content of its DNA is 52·9 mol%. The 16S rRNA gene sequence similarity data show that strain GaT is closely related to members of the genus Geobacillus. Relevant chemotaxonomic data (in particular, the major fatty acid profile of strain GaT, which includes iso-C15 : 0, iso-C16 : 0 and iso-C17 : 0 acids) support the assignment of this strain to the genus Geobacillus. The physiological, biochemical and DNA–DNA hybridization studies of strain GaT showed that it differs both genotypically and phenotypically from the recognized Geobacillus species. Based on these data, strain GaT belongs to a novel species, Geobacillus gargensis sp. nov. (type strain, GaT=VKM B-2300T=DSM 15378T). The analysis of the phenotypic characteristics (additional to those given in the original description) of the type strain of Bacillus vulcani (DSM 13174T) showed that they are very similar to the major phenotypic characteristics of the genus Geobacillus. The low DNA–DNA reassociation values of strain DSM 13174T with various species of this genus (from 38 to 54 %) clearly demonstrate a sufficient genomic distinction of this strain and its taxonomic status as a species. The physiological characteristics, phylogenetic position and DNA–DNA reassociation values of B. vulcani allow this species to be reclassified as Geobacillus vulcani comb. nov. The main properties that differentiate G. vulcani from the other species of the genus are its ability to produce acids from glycerol, lactose and ribose.

  • Geobacillus gargensis sp nov a novel thermophile from a hot spring and the reclassification of bacillus vulcani as Geobacillus vulcani comb nov
    International Journal of Systematic and Evolutionary Microbiology, 2004
    Co-Authors: Tamara N. Nazina, A B Poltaraus, Tatiana P. Tourova, Elena V Lebedeva, Diyana S Sokolova, Anatoliy M Lysenko, Alexandre A Grigoryan, G A Osipov
    Abstract:

    A novel thermophilic spore-forming strain, Ga(T), was isolated from the Garga hot spring located in the northern part of the Transbaikal region (Russia). Strain Ga(T) was found to be an aerobic, Gram-positive, rod-shaped, thermophilic (optimum growth temperature is 60-65 degrees C), chemo-organotrophic bacterium that grows on various sugars, carboxylic acids and hydrocarbons. The G+C content of its DNA is 52.9 mol%. The 16S rRNA gene sequence similarity data show that strain Ga(T) is closely related to members of the genus Geobacillus. Relevant chemotaxonomic data (in particular, the major fatty acid profile of strain Ga(T), which includes iso-C15 : 0, iso-C16 : 0 and iso-C17 : 0 acids) support the assignment of this strain to the genus Geobacillus. The physiological, biochemical and DNA-DNA hybridization studies of strain Ga(T) showed that it differs both genotypically and phenotypically from the recognized Geobacillus species. Based on these data, strain Ga(T) belongs to a novel species, Geobacillus gargensis sp. nov. (type strain, Ga(T)=VKM B-2300(T)=DSM 15378(T)). The analysis of the phenotypic characteristics (additional to those given in the original description) of the type strain of Bacillus vulcani (DSM 13174(T)) showed that they are very similar to the major phenotypic characteristics of the genus Geobacillus. The low DNA-DNA reassociation values of strain DSM 13174(T) with various species of this genus (from 38 to 54 %) clearly demonstrate a sufficient genomic distinction of this strain and its taxonomic status as a species. The physiological characteristics, phylogenetic position and DNA-DNA reassociation values of B. vulcani allow this species to be reclassified as Geobacillus vulcani comb. nov. The main properties that differentiate G. vulcani from the other species of the genus are its ability to produce acids from glycerol, lactose and ribose.