The Experts below are selected from a list of 222 Experts worldwide ranked by ideXlab platform

Mitsuru Furusawa - One of the best experts on this subject based on the ideXlab platform.

  • Immuno‐Localization of DEAD Family Proteins in Germ Line Cells of Xenopus Embryos.
    Development Growth and Differentiation, 1992
    Co-Authors: Masanao Watanabe, Keiji Itoh, Kuniya Abe, Toshifumi Akizawa, Kohji Ikenishi, Mitsuru Furusawa
    Abstract:

    In order to investigate whether a vasa-like protein is present in Germ Line Cells of Xenopus, antibodies were produced which react specifically with synthetic oligopeptides of sequences from near the N- or C-termini or with one including the DEAD box of the Drosophila vasa protein. Only the antibody against the oligopeptide including the DEAD box reacted strongly with Germ plasm (GP) or with cytoplasm of Germ Line Cells of Xenopus embryos by immunofluorescence microscopy. By immunoelectron microscopy, the antibody was demonstrated to react with the GP-specific structure, Germinal granules, in cleaving embryos, and with their derivatives in the Germ Line Cells of embryos at stages extending from gastrula to feeding tadpole. It also reacted with mitochondria not only in the GP and the Germ Line Cells but also in somatic Cells, and with myofibrils in muscle Cells. By Western blotting, the antibody was shown to react with several bands of Mr 42–69 ± 103 in protein samples from Xenopus embryos. In samples from Drosophila ovaries, it reacted with a Mr 71 ± 103 band which was probably the vasa protein. This indicates the possibility that Xenopus embryos contain several DEAD family proteins. One of these is present on Germinal granules, resembling the vasa protein on polar granules of Drosophila.

  • Immuno-Localization of DEAD Family Proteins in Germ Line Cells of Xenopus Embryos. (DEAD protein/vasa-likelGerm Line Cells/Germinal granuleslxenopus)
    1992
    Co-Authors: Masanao Watanabe, Keiji Itoh, Kuniya Abe, Toshifumi Akizawa, Kohji Ikeni, Mitsuru Furusawa
    Abstract:

    ~~ ~~ ~~ ~ In order to investigate whether a vasa-like protein is present in Germ Line Cells of Xenopus, antibodies were produced which react specifically with synthetic oligopeptides of sequences from near the N- or Ctermini or with one including the DEAD box of the Drosophila vasa protein. Only the antibody against the oligopeptide including the DEAD box reacted strongly with Germ plasm (GP) or with cytoplasm of Germ Jine Cells of Xenopus embryos by immunofluorescence mrcroscopy. By immunoelectron microscopy, the antibody was demonstrated to react with the GP-specific structure, Germinal granules, in cleaving embryos, and with their derivatives in the Germ Line Cells of embryos at stages extending from gastrula to feeding tadpole. It also reacted with rnitochondria not only in the GP and the Germ Line Cells but also in somatic Cells, and with myofibrils in muscle Cells. By Western blotting, the antibody was shown to react with several bands of Mr 42-69 x 1 O3 in protein samples from Xenopus embryos. In samples from Drosophila ovaries, it reacted with a Mr 71 x 103 band which was probably the vasa protein. This indicates the possibility that Xenopus embryos contain several DEAD family proteins. One of these is present on Germinal granules, resembling the vasa protein on polar granules of Drosophila.

Niraj K. Tripathy - One of the best experts on this subject based on the ideXlab platform.

  • Genotoxicity testing of two red dyes in the somatic and Germ Line Cells of Drosophila.
    Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, 1995
    Co-Authors: Niraj K. Tripathy, Nabi, Gupta P. Sahu, A. Anand Kumar
    Abstract:

    Two red dyes, rhodamine B and amaranth, were tested for their genotoxic effects in the somatic (wing primordia) and Germ Line Cells of Drosophila melanogaster following the wing spot and the sex-linked recessive lethal tests. Second- and third-instar larvae, carrying suitable genetic markers, were subjected to chronic exposure to different concentrations of the test dyes. The results indicate that rhodamine is genotoxic in both somatic and Germ Line Cells and amaranth is non-genotoxic.

  • Genotoxicity of 2,4-dichlorophenoxyacetic acid tested in somatic and Germ-Line Cells of Drosophila.
    Mutation research, 1993
    Co-Authors: Niraj K. Tripathy, Gupta P. Sahu, Prabhat K. Routray, A. Anand Kumar
    Abstract:

    The genotoxic potential of 2,4-dichlorophenoxyacetic acid, a commonly used chlorophenoxy herbicide, was tested in Drosophila somatic and Germ-Line Cells following the protocols of the wing spot test and the sex-linked recessive lethal test. In the wing spot test second- and third-instar larvae, carrying genetic markers mwh and flr3, were exposed to different concentrations of the herbicide so that induced genetic changes would be phenotypically expressed as mosaic spots on the wings of eclosing adults. The Basc (Muller-5) standard technique but with larval exposure was followed for the sex-linked recessive lethal test. The results obtained indicate that the test compound is genotoxic both in the somatic and Germ-Line Cells of Drosophila.

  • Farm-grade chlorpyrifos (Durmet) is genotoxic in somatic and Germ-Line Cells of Drosophila
    Mutation research, 1992
    Co-Authors: Kalyani K. Patnaik, Niraj K. Tripathy
    Abstract:

    The mutagenic potential of Durmet, a farm-grade formulation of chlorpyrifos, was studied in the Drosophila wing mosaic and sex-linked recessive lethal tests. Larvae of the 2nd or 3rd instar carrying suitable recessive genetic markers on chromosome 3 were exposed to different concentrations of the insecticide and the frequency of induction of mutant mosaic spots on the wings was noted. The Basc technique was followed to study the induction of sex-linked recessive lethals. On the basis of the frequency of induction of mosaic wing spots and sex-linked recessive lethals, it is concluded that Durmet is genotoxic in somatic Cells as well as Germ Cells of Drosophila.

  • Studies on the genotoxicity of monocrotophos in somatic and Germ-Line Cells of drosophila
    Mutation research, 1992
    Co-Authors: Niraj K. Tripathy, Kalyani K. Patnaik
    Abstract:

    Abstract Parryfos, a farm-grade formulation of monocrotophos, was tested for genotoxicity in the wing primordial Cells and the male Germ-Line Cells of Drosophila melanogaster . Larvae of the 2nd or 3rd instar. heterozygous for the wing-cell marker mutations mwh and flr 3 were exposed to different concentrations of the insecticide in the food. The wings of the hatched flies were screened for the presence of mutant mosaic spots exhibiting the marker phenotypes. The frequency of induction of sex-linked recessive lethal mutations was used to assess genotoxic effects in male Germ-Line Cells. The tested compound was genotoxic in both the somatic and the Germ-Line Cells of Drosophila.

Toshiaki Noce - One of the best experts on this subject based on the ideXlab platform.

  • differences and similarities in the developmental status of embryo derived stem Cells and primordial Germ Cells revealed by global expression profiling
    Genes to Cells, 2008
    Co-Authors: Nathan Mise, Takuya Fuchikami, Michihiko Sugimoto, Satoru Kobayakawa, Takehiko Ogawa, Takashi Tada, Shigehiko Kanaya, Toshiaki Noce
    Abstract:

    Embryonic Germ-Line Cells are unipotent Cells that give rise to either sperm or oocytes. However, pluripotent stem Cells can be derived from primordial Germ Cells (PGCs) or spermatogonia, suggesting that Germ-Line Cells retain a capacity for pluripotency. Here, we made genome-wide comparisons of the gene expression profiles of freshly isolated PGCs, in vitro-formed PGCs (iPGCs), and other stem cell Lines, including embryonic stem Cells (ESCs), embryonic Germ Cells (EGCs) and Germ-Line stem (GS) Cells. Comparing PGC with ESC, 382 genes/transcripts were significantly up-regulated in ESC, while 188 were elevated in PGC. This suggests that PGCs possess transcription program distinct from that of ESC, although both share expression of many pluripotency-associated genes. Our micro-array analysis showed that the analyzed samples could be classified into two groups: one consisting of all the ESCs and most of EGCs, and the other containing PGC samples, iPGC, one type of female EGC and GS Cells. We then identified “signature” genes for the two groups, and used them to characterize GS Cells, EGC, and iPGCs, and revealed developmental status of each cell type. The relationships between PGCs and stem Cells derived from embryos or Germ Cells are discussed in light of these findings.

Hannu Alho - One of the best experts on this subject based on the ideXlab platform.

  • the transcriptional and translational control of diazepam binding inhibitor expression in rat male Germ Line Cells
    DNA and Cell Biology, 1997
    Co-Authors: Meelis Kolmer, Markku Peltohuikko, Martti Parvinen, Christer Hoog, Hannu Alho
    Abstract:

    The diazepam binding inhibitor [DBI, also known as acyl-CoA-binding protein, (ACBP), or endozepine] is a 10-kD protein that has been suggested to be involved in the regulation of several biological processes such as acyl-CoA metabolism, steroidogenesis, insulin secretion, and γ-aminobutyric acid type A (GABAA)/benzodiazepine receptor modulation. DBI has been cloned from vertebrates, insects, plants, and yeasts. In mammals, DBI is expressed in almost all the tissues studied. Nevertheless, DBI expression is restricted to specific cell types. Here we have studied DBI gene expression in the Germ-Line Cells of rat testis. The DBI gene was intensively transcribed in postmeiotic round spermatids from stages VI to VIII of the seminiferous epithelial cycle. A prominent, spermatid-specific upstream transcription initiation site was identified in addition to the multiple common transcriptional initiation sites found in the somatic tissues. However, no DBI protein was detected in round spermatids, suggesting that the...

Masanao Watanabe - One of the best experts on this subject based on the ideXlab platform.

  • Immuno‐Localization of DEAD Family Proteins in Germ Line Cells of Xenopus Embryos.
    Development Growth and Differentiation, 1992
    Co-Authors: Masanao Watanabe, Keiji Itoh, Kuniya Abe, Toshifumi Akizawa, Kohji Ikenishi, Mitsuru Furusawa
    Abstract:

    In order to investigate whether a vasa-like protein is present in Germ Line Cells of Xenopus, antibodies were produced which react specifically with synthetic oligopeptides of sequences from near the N- or C-termini or with one including the DEAD box of the Drosophila vasa protein. Only the antibody against the oligopeptide including the DEAD box reacted strongly with Germ plasm (GP) or with cytoplasm of Germ Line Cells of Xenopus embryos by immunofluorescence microscopy. By immunoelectron microscopy, the antibody was demonstrated to react with the GP-specific structure, Germinal granules, in cleaving embryos, and with their derivatives in the Germ Line Cells of embryos at stages extending from gastrula to feeding tadpole. It also reacted with mitochondria not only in the GP and the Germ Line Cells but also in somatic Cells, and with myofibrils in muscle Cells. By Western blotting, the antibody was shown to react with several bands of Mr 42–69 ± 103 in protein samples from Xenopus embryos. In samples from Drosophila ovaries, it reacted with a Mr 71 ± 103 band which was probably the vasa protein. This indicates the possibility that Xenopus embryos contain several DEAD family proteins. One of these is present on Germinal granules, resembling the vasa protein on polar granules of Drosophila.

  • Immuno-Localization of DEAD Family Proteins in Germ Line Cells of Xenopus Embryos. (DEAD protein/vasa-likelGerm Line Cells/Germinal granuleslxenopus)
    1992
    Co-Authors: Masanao Watanabe, Keiji Itoh, Kuniya Abe, Toshifumi Akizawa, Kohji Ikeni, Mitsuru Furusawa
    Abstract:

    ~~ ~~ ~~ ~ In order to investigate whether a vasa-like protein is present in Germ Line Cells of Xenopus, antibodies were produced which react specifically with synthetic oligopeptides of sequences from near the N- or Ctermini or with one including the DEAD box of the Drosophila vasa protein. Only the antibody against the oligopeptide including the DEAD box reacted strongly with Germ plasm (GP) or with cytoplasm of Germ Jine Cells of Xenopus embryos by immunofluorescence mrcroscopy. By immunoelectron microscopy, the antibody was demonstrated to react with the GP-specific structure, Germinal granules, in cleaving embryos, and with their derivatives in the Germ Line Cells of embryos at stages extending from gastrula to feeding tadpole. It also reacted with rnitochondria not only in the GP and the Germ Line Cells but also in somatic Cells, and with myofibrils in muscle Cells. By Western blotting, the antibody was shown to react with several bands of Mr 42-69 x 1 O3 in protein samples from Xenopus embryos. In samples from Drosophila ovaries, it reacted with a Mr 71 x 103 band which was probably the vasa protein. This indicates the possibility that Xenopus embryos contain several DEAD family proteins. One of these is present on Germinal granules, resembling the vasa protein on polar granules of Drosophila.