The Experts below are selected from a list of 15 Experts worldwide ranked by ideXlab platform
Juan M. Tomás - One of the best experts on this subject based on the ideXlab platform.
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The role of the O-antigen lipopolysaccharide on the colonizationin vivoof the Germfree Chicken gut byAeromonas hydrophilaserogroup O:34
Microbial pathogenesis, 1996Co-Authors: Susana Merino, Xavier Rubires, Alicia Aguillar, Jean Guillot, Juan M. TomásAbstract:Abstract We compared the ability of different Aeromonas hydrophila strains from serogroup O:34 grown at different temperatures to colonize in vivo the Germfree Chicken gut. We found a good colonization when the strains were grown at 20°C but not when they were grown at 37°C. We previously described that these strains were able to form the O-antigen lipopolysaccharide (LPS) when they grow at low temperature but not at high temperature. We also obtained by transposon mutagenesis mutants only devoid of the O-antigen LPS ( rfb mutants), and showed that they were unable to colonize the Germfree Chicken gut. All these results prompted us to conclude that the O-antigen LPS, in these strains, is a main factor for colonization in this animal model system.
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The role of the O-antigen lipopolysaccharide on the colonization in vivo of the Germfree Chicken gut by Klebsiella pneumoniae
Microbial pathogenesis, 1993Co-Authors: Silvia Camprubi, Susana Merino, Jean F. Guillot, Juan M. TomásAbstract:We isolated lipopolysaccharide and capsular polysaccharide (K antigen)-defective mutants from two Klebsiella pneumoniae parental strains, and compared their ability to colonize in vivo the Germfree Chicken gut. The high-molecular weight lipopolysaccharide (LPS) (O antigen) was found necessary for the colonization while the capsular polysaccharide (K2 or K29) was not of importance.
Surang Chankhamhaengdecha - One of the best experts on this subject based on the ideXlab platform.
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Gut Microbial Dynamics during Conventionalization of Germfree Chicken
mSphere, 2019Co-Authors: Milton Thomas, Supapit Wongkuna, Sudeep Ghimire, Roshan Kumar, Linto Antony, Kinchel C. Doerner, Aaron Singery, Eric A. Nelson, Tofuko A Woyengo, Surang ChankhamhaengdechaAbstract:ABSTRACT A gnotobiotic Gallus gallus (Chicken) model was developed to study the dynamics of intestinal microflora from hatching to 18 days of age employing metagenomics. Intestinal samples were collected from a local population of feral Chickens and administered orally to Germfree 3-day-old chicks. Animals were euthanized on days 9 and 18 postinoculation, and intestinal samples were collected and subjected to metagenomic analysis. On day 18, the five most prevalent phyla were Bacteroidetes (43.03 ± 3.19%), Firmicutes (38.51 ± 2.67%), Actinobacteria (6.77 ± 0.7%), Proteobacteria (6.38 ± 0.7%), and Spirochaetes (2.71 ± 0.55%). Principal-coordinate analysis showed that the day 18 variables clustered more closely than the day 9 variables, suggesting that the microbial communities had changed temporally. The Morista-Horn index values ranged from 0.7 to 1, indicating that the communities in the inoculum and in the day 9 and day 18 samples were more similar than dissimilar. The predicted functional profiles of the microbiomes of the inoculum and the day 9 and day 18 samples were also similar (values of 0.98 to 1). These results indicate that the gnotobiotic chicks stably maintained the phylogenetic diversity and predicted metabolic functionality of the inoculum community. IMPORTANCE The domestic Chicken is the cornerstone of animal agriculture worldwide, with a flock population exceeding 40 billion birds/year. It serves as an economically valuable source of protein globally. The microbiome of poultry has important effects on Chicken growth, feed conversion, immune status, and pathogen resistance. The aim of our research was to develop a gnotobiotic Chicken model appropriate for the study Chicken gut microbiota function. Our experimental model shows that young Germfree chicks are able to colonize diverse sets of gut bacteria. Therefore, besides the use of this model to study mechanisms of gut microbiota interactions in the Chicken gut, it could be also used for applied aspects such as determining the safety and efficacy of new probiotic strains derived from Chicken gut microbiota.
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Gut Microbial Dynamics during Conventionalization of Germfree Chicken
American Society for Microbiology, 2019Co-Authors: Milton Thomas, Supapit Wongkuna, Sudeep Ghimire, Roshan Kumar, Linto Antony, Kinchel C. Doerner, Aaron Singery, Tofuko A Woyengo, Eric Nelson, Surang ChankhamhaengdechaAbstract:The domestic Chicken is the cornerstone of animal agriculture worldwide, with a flock population exceeding 40 billion birds/year. It serves as an economically valuable source of protein globally. The microbiome of poultry has important effects on Chicken growth, feed conversion, immune status, and pathogen resistance. The aim of our research was to develop a gnotobiotic Chicken model appropriate for the study Chicken gut microbiota function. Our experimental model shows that young Germfree chicks are able to colonize diverse sets of gut bacteria. Therefore, besides the use of this model to study mechanisms of gut microbiota interactions in the Chicken gut, it could be also used for applied aspects such as determining the safety and efficacy of new probiotic strains derived from Chicken gut microbiota.A gnotobiotic Gallus gallus (Chicken) model was developed to study the dynamics of intestinal microflora from hatching to 18 days of age employing metagenomics. Intestinal samples were collected from a local population of feral Chickens and administered orally to Germfree 3-day-old chicks. Animals were euthanized on days 9 and 18 postinoculation, and intestinal samples were collected and subjected to metagenomic analysis. On day 18, the five most prevalent phyla were Bacteroidetes (43.03 ± 3.19%), Firmicutes (38.51 ± 2.67%), Actinobacteria (6.77 ± 0.7%), Proteobacteria (6.38 ± 0.7%), and Spirochaetes (2.71 ± 0.55%). Principal-coordinate analysis showed that the day 18 variables clustered more closely than the day 9 variables, suggesting that the microbial communities had changed temporally. The Morista-Horn index values ranged from 0.7 to 1, indicating that the communities in the inoculum and in the day 9 and day 18 samples were more similar than dissimilar. The predicted functional profiles of the microbiomes of the inoculum and the day 9 and day 18 samples were also similar (values of 0.98 to 1). These results indicate that the gnotobiotic chicks stably maintained the phylogenetic diversity and predicted metabolic functionality of the inoculum community
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Gut Microbial succession during conventionalization of Germfree Chicken
2018Co-Authors: Milton Thomas, Supapit Wongkuna, Sudeep Ghimire, Kinchel C. Doerner, Aaron Singery, Eric A. Nelson, Tofuko A Woyengo, Surang Chankhamhaengdecha, Tavan Janvilisri, Joy ScariaAbstract:A gnotobiotic Chicken model was developed to study the succession of intestinal microflora from hatching to 18 days of age. Intestinal samples were collected from a local population of feral Chickens and administered orally to germ-free 3 day old chicks. Animals were enthanized on 0, 9 and 18 days of age and intestinal samples were collected and subjected to genomic analysis. The five most prevalent phyla were Bacteroidetes (45.73%), Firmicutes (36.47%), Proteobacteria (8.28%), Actinobacteria (5.09%), and Spriochetes (2.10%). Principle coordinate analysis indicated the 0, 9 day and 18 day variables clustered together and the microbial communities changed temporally. The Morista-Horn index values ranged from 0.72 to 1, indicating the communities at 0, 9 or 18 days were more similar than dissimilar. The predicted functional profiles of the microbiomes of 0, 9 and 18 days were also similar. These results indicate the gnotobiotic chicks stably maintain the phylogentic diversity and predicted metabolic functionality of the inoculum community. Importance: The domestic Chicken is the cornerstone of animal agriculture worldwide with a flock population exceeding 40 billion birds/year. It serves as the economically valuable source of protein globally. Microbiome of poultry has important effects on Chicken growth, feed conversion, immune status and pathogen resistance. The significance of our research is in developing a gnotobiotic Chicken model to study Chicken gut microbiota function. Our experimental model shows that young Germfree chicks are able to colonize diverse set of gut bacteria. Therefore, besides using this model to study mechanisms of gut microbiota interactions in the Chicken gut, our model could be also used for applied aspects such as determining the safety and efficacy of new probiotic strains derived from Chicken gut microbiota.
Milton Thomas - One of the best experts on this subject based on the ideXlab platform.
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Gut Microbial Dynamics during Conventionalization of Germfree Chicken
mSphere, 2019Co-Authors: Milton Thomas, Supapit Wongkuna, Sudeep Ghimire, Roshan Kumar, Linto Antony, Kinchel C. Doerner, Aaron Singery, Eric A. Nelson, Tofuko A Woyengo, Surang ChankhamhaengdechaAbstract:ABSTRACT A gnotobiotic Gallus gallus (Chicken) model was developed to study the dynamics of intestinal microflora from hatching to 18 days of age employing metagenomics. Intestinal samples were collected from a local population of feral Chickens and administered orally to Germfree 3-day-old chicks. Animals were euthanized on days 9 and 18 postinoculation, and intestinal samples were collected and subjected to metagenomic analysis. On day 18, the five most prevalent phyla were Bacteroidetes (43.03 ± 3.19%), Firmicutes (38.51 ± 2.67%), Actinobacteria (6.77 ± 0.7%), Proteobacteria (6.38 ± 0.7%), and Spirochaetes (2.71 ± 0.55%). Principal-coordinate analysis showed that the day 18 variables clustered more closely than the day 9 variables, suggesting that the microbial communities had changed temporally. The Morista-Horn index values ranged from 0.7 to 1, indicating that the communities in the inoculum and in the day 9 and day 18 samples were more similar than dissimilar. The predicted functional profiles of the microbiomes of the inoculum and the day 9 and day 18 samples were also similar (values of 0.98 to 1). These results indicate that the gnotobiotic chicks stably maintained the phylogenetic diversity and predicted metabolic functionality of the inoculum community. IMPORTANCE The domestic Chicken is the cornerstone of animal agriculture worldwide, with a flock population exceeding 40 billion birds/year. It serves as an economically valuable source of protein globally. The microbiome of poultry has important effects on Chicken growth, feed conversion, immune status, and pathogen resistance. The aim of our research was to develop a gnotobiotic Chicken model appropriate for the study Chicken gut microbiota function. Our experimental model shows that young Germfree chicks are able to colonize diverse sets of gut bacteria. Therefore, besides the use of this model to study mechanisms of gut microbiota interactions in the Chicken gut, it could be also used for applied aspects such as determining the safety and efficacy of new probiotic strains derived from Chicken gut microbiota.
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Gut Microbial Dynamics during Conventionalization of Germfree Chicken
American Society for Microbiology, 2019Co-Authors: Milton Thomas, Supapit Wongkuna, Sudeep Ghimire, Roshan Kumar, Linto Antony, Kinchel C. Doerner, Aaron Singery, Tofuko A Woyengo, Eric Nelson, Surang ChankhamhaengdechaAbstract:The domestic Chicken is the cornerstone of animal agriculture worldwide, with a flock population exceeding 40 billion birds/year. It serves as an economically valuable source of protein globally. The microbiome of poultry has important effects on Chicken growth, feed conversion, immune status, and pathogen resistance. The aim of our research was to develop a gnotobiotic Chicken model appropriate for the study Chicken gut microbiota function. Our experimental model shows that young Germfree chicks are able to colonize diverse sets of gut bacteria. Therefore, besides the use of this model to study mechanisms of gut microbiota interactions in the Chicken gut, it could be also used for applied aspects such as determining the safety and efficacy of new probiotic strains derived from Chicken gut microbiota.A gnotobiotic Gallus gallus (Chicken) model was developed to study the dynamics of intestinal microflora from hatching to 18 days of age employing metagenomics. Intestinal samples were collected from a local population of feral Chickens and administered orally to Germfree 3-day-old chicks. Animals were euthanized on days 9 and 18 postinoculation, and intestinal samples were collected and subjected to metagenomic analysis. On day 18, the five most prevalent phyla were Bacteroidetes (43.03 ± 3.19%), Firmicutes (38.51 ± 2.67%), Actinobacteria (6.77 ± 0.7%), Proteobacteria (6.38 ± 0.7%), and Spirochaetes (2.71 ± 0.55%). Principal-coordinate analysis showed that the day 18 variables clustered more closely than the day 9 variables, suggesting that the microbial communities had changed temporally. The Morista-Horn index values ranged from 0.7 to 1, indicating that the communities in the inoculum and in the day 9 and day 18 samples were more similar than dissimilar. The predicted functional profiles of the microbiomes of the inoculum and the day 9 and day 18 samples were also similar (values of 0.98 to 1). These results indicate that the gnotobiotic chicks stably maintained the phylogenetic diversity and predicted metabolic functionality of the inoculum community
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Gut Microbial succession during conventionalization of Germfree Chicken
2018Co-Authors: Milton Thomas, Supapit Wongkuna, Sudeep Ghimire, Kinchel C. Doerner, Aaron Singery, Eric A. Nelson, Tofuko A Woyengo, Surang Chankhamhaengdecha, Tavan Janvilisri, Joy ScariaAbstract:A gnotobiotic Chicken model was developed to study the succession of intestinal microflora from hatching to 18 days of age. Intestinal samples were collected from a local population of feral Chickens and administered orally to germ-free 3 day old chicks. Animals were enthanized on 0, 9 and 18 days of age and intestinal samples were collected and subjected to genomic analysis. The five most prevalent phyla were Bacteroidetes (45.73%), Firmicutes (36.47%), Proteobacteria (8.28%), Actinobacteria (5.09%), and Spriochetes (2.10%). Principle coordinate analysis indicated the 0, 9 day and 18 day variables clustered together and the microbial communities changed temporally. The Morista-Horn index values ranged from 0.72 to 1, indicating the communities at 0, 9 or 18 days were more similar than dissimilar. The predicted functional profiles of the microbiomes of 0, 9 and 18 days were also similar. These results indicate the gnotobiotic chicks stably maintain the phylogentic diversity and predicted metabolic functionality of the inoculum community. Importance: The domestic Chicken is the cornerstone of animal agriculture worldwide with a flock population exceeding 40 billion birds/year. It serves as the economically valuable source of protein globally. Microbiome of poultry has important effects on Chicken growth, feed conversion, immune status and pathogen resistance. The significance of our research is in developing a gnotobiotic Chicken model to study Chicken gut microbiota function. Our experimental model shows that young Germfree chicks are able to colonize diverse set of gut bacteria. Therefore, besides using this model to study mechanisms of gut microbiota interactions in the Chicken gut, our model could be also used for applied aspects such as determining the safety and efficacy of new probiotic strains derived from Chicken gut microbiota.
Susana Merino - One of the best experts on this subject based on the ideXlab platform.
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The role of the O-antigen lipopolysaccharide on the colonizationin vivoof the Germfree Chicken gut byAeromonas hydrophilaserogroup O:34
Microbial pathogenesis, 1996Co-Authors: Susana Merino, Xavier Rubires, Alicia Aguillar, Jean Guillot, Juan M. TomásAbstract:Abstract We compared the ability of different Aeromonas hydrophila strains from serogroup O:34 grown at different temperatures to colonize in vivo the Germfree Chicken gut. We found a good colonization when the strains were grown at 20°C but not when they were grown at 37°C. We previously described that these strains were able to form the O-antigen lipopolysaccharide (LPS) when they grow at low temperature but not at high temperature. We also obtained by transposon mutagenesis mutants only devoid of the O-antigen LPS ( rfb mutants), and showed that they were unable to colonize the Germfree Chicken gut. All these results prompted us to conclude that the O-antigen LPS, in these strains, is a main factor for colonization in this animal model system.
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The role of the O-antigen lipopolysaccharide on the colonization in vivo of the Germfree Chicken gut by Klebsiella pneumoniae
Microbial pathogenesis, 1993Co-Authors: Silvia Camprubi, Susana Merino, Jean F. Guillot, Juan M. TomásAbstract:We isolated lipopolysaccharide and capsular polysaccharide (K antigen)-defective mutants from two Klebsiella pneumoniae parental strains, and compared their ability to colonize in vivo the Germfree Chicken gut. The high-molecular weight lipopolysaccharide (LPS) (O antigen) was found necessary for the colonization while the capsular polysaccharide (K2 or K29) was not of importance.
Tofuko A Woyengo - One of the best experts on this subject based on the ideXlab platform.
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Gut Microbial Dynamics during Conventionalization of Germfree Chicken
mSphere, 2019Co-Authors: Milton Thomas, Supapit Wongkuna, Sudeep Ghimire, Roshan Kumar, Linto Antony, Kinchel C. Doerner, Aaron Singery, Eric A. Nelson, Tofuko A Woyengo, Surang ChankhamhaengdechaAbstract:ABSTRACT A gnotobiotic Gallus gallus (Chicken) model was developed to study the dynamics of intestinal microflora from hatching to 18 days of age employing metagenomics. Intestinal samples were collected from a local population of feral Chickens and administered orally to Germfree 3-day-old chicks. Animals were euthanized on days 9 and 18 postinoculation, and intestinal samples were collected and subjected to metagenomic analysis. On day 18, the five most prevalent phyla were Bacteroidetes (43.03 ± 3.19%), Firmicutes (38.51 ± 2.67%), Actinobacteria (6.77 ± 0.7%), Proteobacteria (6.38 ± 0.7%), and Spirochaetes (2.71 ± 0.55%). Principal-coordinate analysis showed that the day 18 variables clustered more closely than the day 9 variables, suggesting that the microbial communities had changed temporally. The Morista-Horn index values ranged from 0.7 to 1, indicating that the communities in the inoculum and in the day 9 and day 18 samples were more similar than dissimilar. The predicted functional profiles of the microbiomes of the inoculum and the day 9 and day 18 samples were also similar (values of 0.98 to 1). These results indicate that the gnotobiotic chicks stably maintained the phylogenetic diversity and predicted metabolic functionality of the inoculum community. IMPORTANCE The domestic Chicken is the cornerstone of animal agriculture worldwide, with a flock population exceeding 40 billion birds/year. It serves as an economically valuable source of protein globally. The microbiome of poultry has important effects on Chicken growth, feed conversion, immune status, and pathogen resistance. The aim of our research was to develop a gnotobiotic Chicken model appropriate for the study Chicken gut microbiota function. Our experimental model shows that young Germfree chicks are able to colonize diverse sets of gut bacteria. Therefore, besides the use of this model to study mechanisms of gut microbiota interactions in the Chicken gut, it could be also used for applied aspects such as determining the safety and efficacy of new probiotic strains derived from Chicken gut microbiota.
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Gut Microbial Dynamics during Conventionalization of Germfree Chicken
American Society for Microbiology, 2019Co-Authors: Milton Thomas, Supapit Wongkuna, Sudeep Ghimire, Roshan Kumar, Linto Antony, Kinchel C. Doerner, Aaron Singery, Tofuko A Woyengo, Eric Nelson, Surang ChankhamhaengdechaAbstract:The domestic Chicken is the cornerstone of animal agriculture worldwide, with a flock population exceeding 40 billion birds/year. It serves as an economically valuable source of protein globally. The microbiome of poultry has important effects on Chicken growth, feed conversion, immune status, and pathogen resistance. The aim of our research was to develop a gnotobiotic Chicken model appropriate for the study Chicken gut microbiota function. Our experimental model shows that young Germfree chicks are able to colonize diverse sets of gut bacteria. Therefore, besides the use of this model to study mechanisms of gut microbiota interactions in the Chicken gut, it could be also used for applied aspects such as determining the safety and efficacy of new probiotic strains derived from Chicken gut microbiota.A gnotobiotic Gallus gallus (Chicken) model was developed to study the dynamics of intestinal microflora from hatching to 18 days of age employing metagenomics. Intestinal samples were collected from a local population of feral Chickens and administered orally to Germfree 3-day-old chicks. Animals were euthanized on days 9 and 18 postinoculation, and intestinal samples were collected and subjected to metagenomic analysis. On day 18, the five most prevalent phyla were Bacteroidetes (43.03 ± 3.19%), Firmicutes (38.51 ± 2.67%), Actinobacteria (6.77 ± 0.7%), Proteobacteria (6.38 ± 0.7%), and Spirochaetes (2.71 ± 0.55%). Principal-coordinate analysis showed that the day 18 variables clustered more closely than the day 9 variables, suggesting that the microbial communities had changed temporally. The Morista-Horn index values ranged from 0.7 to 1, indicating that the communities in the inoculum and in the day 9 and day 18 samples were more similar than dissimilar. The predicted functional profiles of the microbiomes of the inoculum and the day 9 and day 18 samples were also similar (values of 0.98 to 1). These results indicate that the gnotobiotic chicks stably maintained the phylogenetic diversity and predicted metabolic functionality of the inoculum community
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Gut Microbial succession during conventionalization of Germfree Chicken
2018Co-Authors: Milton Thomas, Supapit Wongkuna, Sudeep Ghimire, Kinchel C. Doerner, Aaron Singery, Eric A. Nelson, Tofuko A Woyengo, Surang Chankhamhaengdecha, Tavan Janvilisri, Joy ScariaAbstract:A gnotobiotic Chicken model was developed to study the succession of intestinal microflora from hatching to 18 days of age. Intestinal samples were collected from a local population of feral Chickens and administered orally to germ-free 3 day old chicks. Animals were enthanized on 0, 9 and 18 days of age and intestinal samples were collected and subjected to genomic analysis. The five most prevalent phyla were Bacteroidetes (45.73%), Firmicutes (36.47%), Proteobacteria (8.28%), Actinobacteria (5.09%), and Spriochetes (2.10%). Principle coordinate analysis indicated the 0, 9 day and 18 day variables clustered together and the microbial communities changed temporally. The Morista-Horn index values ranged from 0.72 to 1, indicating the communities at 0, 9 or 18 days were more similar than dissimilar. The predicted functional profiles of the microbiomes of 0, 9 and 18 days were also similar. These results indicate the gnotobiotic chicks stably maintain the phylogentic diversity and predicted metabolic functionality of the inoculum community. Importance: The domestic Chicken is the cornerstone of animal agriculture worldwide with a flock population exceeding 40 billion birds/year. It serves as the economically valuable source of protein globally. Microbiome of poultry has important effects on Chicken growth, feed conversion, immune status and pathogen resistance. The significance of our research is in developing a gnotobiotic Chicken model to study Chicken gut microbiota function. Our experimental model shows that young Germfree chicks are able to colonize diverse set of gut bacteria. Therefore, besides using this model to study mechanisms of gut microbiota interactions in the Chicken gut, our model could be also used for applied aspects such as determining the safety and efficacy of new probiotic strains derived from Chicken gut microbiota.