The Experts below are selected from a list of 5667 Experts worldwide ranked by ideXlab platform
Fujun Shen - One of the best experts on this subject based on the ideXlab platform.
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transcriptome derived tetranucleotide microsatellites and their associated genes from the Giant Panda ailuropoda melanoleuca
Journal of Heredity, 2016Co-Authors: Xuhao Song, Fujun Shen, Chengdong Wang, Jie Huang, Yan Huang, Lianming Du, Xiuyue ZhangAbstract:: Recently, an increasing number of microsatellites or simple sequence repeats (SSRs) have been found and characterized from transcriptomes. Such SSRs can be employed as putative functional markers to easily tag corresponding genes, which play an important role in biomedical studies and genetic analysis. However, the transcriptome-derived SSRs for Giant Panda (Ailuropoda melanoleuca) are not yet available. In this work, we identified and characterized 20 tetranucleotide microsatellite loci from a transcript database generated from the blood of Giant Panda. Furthermore, we assigned their predicted transcriptome locations: 16 loci were assigned to untranslated regions (UTRs) and 4 loci were assigned to coding regions (CDSs). Gene identities of 14 transcripts contained corresponding microsatellites were determined, which provide useful information to study the potential contribution of SSRs to gene regulation in Giant Panda. The polymorphic information content (PIC) values ranged from 0.293 to 0.789 with an average of 0.603 for the 16 UTRs-derived SSRs. Interestingly, 4 CDS-derived microsatellites developed in our study were also polymorphic, and the instability of these 4 CDS-derived SSRs was further validated by re-genotyping and sequencing. The genes containing these 4 CDS-derived SSRs were embedded with various types of repeat motifs. The interaction of all the length-changing SSRs might provide a way against coding region frameshift caused by microsatellite instability. We hope these newly gene-associated biomarkers will pave the way for genetic and biomedical studies for Giant Panda in the future. In sum, this set of transcriptome-derived markers complements the genetic resources available for Giant Panda.
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Development of new tetranucleotide microsatellite loci and assessment of genetic variation of Giant Panda in two largest Giant Panda captive breeding populations
Journal of Zoology, 2010Co-Authors: Fujun Shen, Zhihe Zhang, Wenping Zhang, Hou Rong, Bisong YueAbstract:The Giant Panda Ailuropoda melanoleuca is a critically endangered species endemic to China. In order to carry out effective genetic management for the Giant Panda population, sufficient and reliable polymorphic genetic markers are required to provide essential information on the genetic diversity survey of this species. Seven new tetranucleotide microsatellite loci were isolated and characterized in this study and presented here as a tool for evaluating the genetic variation of Giant Pandas in the world's two largest captive populations (Chengdu Research Base of Giant Panda Breeding, Sichuan Province and the China Research and Conservation Center for the Giant Panda in Wolong, Sichuan Province). A total of 45 alleles were identified from these seven new microsatellite loci on the basis of 48 Giant Panda individuals, including 19 private alleles (six from the Chengdu population, 13 from the Wolong population) and 26 shared alleles. The average number of alleles, the average allelic richness the and mean observed heterozygosity were 4.6, 4.367 and 0.649, respectively in the Chengdu population and 5.6, 5.697 and 0.675 in the Wolong population, suggesting that the Chengdu population has a much lower allelic diversity than the Wolong population. Thus, we proposed a better strategy for the captive breeding of Giant Pandas.
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Di-, tri- and tetranucleotide microsatellite loci for the Giant Panda, Ailuropoda melanoleuca
Molecular Ecology Notes, 2007Co-Authors: Fujun Shen, Anju. Zhang, Stephanie Sanderson, Phillip C. Watts, Wei He, Zhihe Zhang, Stephen J. KempAbstract:We describe 10 polymorphic microsatellite loci for the Giant Panda, Ailuropoda melanoleuca . Microsatellite sequences were isolated from three partial genomic libraries of Giant Panda DNA that were enriched for (i) (GT), (ii) (GAA) & (CAA), and (iii) (GATA) repeat sequences. The markers were tested for polymorphism in up to 82 Pandas. Number of alleles at each locus varied between four and 11, and the observed and expected heterozygosities varied between 0.267 and 0.732, and between 0.601 and 0.799, respectively.
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Paternity assignment of Giant Panda by microsatellite genotyping
Yi chuan = Hereditas, 2003Co-Authors: Zhihe Zhang, Anju. Zhang, Fujun Shen, Shan Sun, Victor A. David, Stephen J. O'brienAbstract:We used genotype data across 18 microsatellite loci to establish the paternity of Giant Panda cubs at the Chengdu Zoo and the Chengdu Research Base of Giant Panda Breeding. The results demonstrate that the combined exclusion probability using these 18 microsatellite loci is 0.999921 while confidence is 95 % when the mother is known; if mother is unknown then the exclusion probability is 0.994109, but the confidence of this is less than 80%. Since the mother-offspring relationship is known in captive populations, the results could resolve unknown paternities in the Chengdu ex situ populations. However, to establish accurately the genetic relationships of wild Giant Pandas,more microsatellite loci may be required.
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Pedigree analysis of captive Giant Panda
Yi chuan xue bao = Acta genetica Sinica, 2002Co-Authors: Fujun Shen, Zhihe Zhang, Anju. ZhangAbstract:Abstract Pedigree analysis of captive Giant Panda was conducted by Sparks Ver1.4 Software. The result shows that genetic drift has a strong effect on the loss of genetic diversity. And the dispersal of captive Giant Panda is an acute dangerous factor when all of these small populations would be declined if inbreeding happened. For this reason, all these small populations should be managed as a whole unit to approach the goal for long-term conservation.
Dongwei Kang - One of the best experts on this subject based on the ideXlab platform.
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Giant Panda protection: challenges and hopes.
Environmental science and pollution research international, 2019Co-Authors: Dongwei KangAbstract:In this paper, we discussed some major issues that hinder Giant Panda protection, such as diverse and mixed threats, habitat fragmentation, as well as the survey method to be improved, and some new protection actions appeared, such as the pilot program for the Giant Panda national park system and the administration of the Giant Panda national park was established. These information could provide important information for Giant Panda protection.
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Role of nature reserves in Giant Panda protection.
Environmental science and pollution research international, 2017Co-Authors: Dongwei KangAbstract:Giant Panda (Ailuropoda melanoleuca) is a flagship species in nature conservation of the world; to protect this species, 67 nature reserves have been established in China. To evaluate the protection effect of Giant Panda nature reserves, we analyzed the variation of Giant Panda number and habitat area of 23 Giant Panda nature reserves of Sichuan province based on the national survey data released by State Forestry Administration and Sichuan Forestry Department. Results showed that from the third national survey to the fourth, Giant Panda number and habitat area of 23 Giant Panda nature reserves of Sichuan province failed to realize the significant increase. Furthermore, we found that the total population growth rate of 23 nature reserves in the last 12 years was lower than those of the province total of Sichuan and the national total of China, and the total habitat area of the 23 nature reserves was decreasing in the last 12 years, but the province total and national total were all increasing. We propose that Giant Panda protection should pay more attention to how to improve the protective effects of nature reserves.
Zhihe Zhang - One of the best experts on this subject based on the ideXlab platform.
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Development of new tetranucleotide microsatellite loci and assessment of genetic variation of Giant Panda in two largest Giant Panda captive breeding populations
Journal of Zoology, 2010Co-Authors: Fujun Shen, Zhihe Zhang, Wenping Zhang, Hou Rong, Bisong YueAbstract:The Giant Panda Ailuropoda melanoleuca is a critically endangered species endemic to China. In order to carry out effective genetic management for the Giant Panda population, sufficient and reliable polymorphic genetic markers are required to provide essential information on the genetic diversity survey of this species. Seven new tetranucleotide microsatellite loci were isolated and characterized in this study and presented here as a tool for evaluating the genetic variation of Giant Pandas in the world's two largest captive populations (Chengdu Research Base of Giant Panda Breeding, Sichuan Province and the China Research and Conservation Center for the Giant Panda in Wolong, Sichuan Province). A total of 45 alleles were identified from these seven new microsatellite loci on the basis of 48 Giant Panda individuals, including 19 private alleles (six from the Chengdu population, 13 from the Wolong population) and 26 shared alleles. The average number of alleles, the average allelic richness the and mean observed heterozygosity were 4.6, 4.367 and 0.649, respectively in the Chengdu population and 5.6, 5.697 and 0.675 in the Wolong population, suggesting that the Chengdu population has a much lower allelic diversity than the Wolong population. Thus, we proposed a better strategy for the captive breeding of Giant Pandas.
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Di-, tri- and tetranucleotide microsatellite loci for the Giant Panda, Ailuropoda melanoleuca
Molecular Ecology Notes, 2007Co-Authors: Fujun Shen, Anju. Zhang, Stephanie Sanderson, Phillip C. Watts, Wei He, Zhihe Zhang, Stephen J. KempAbstract:We describe 10 polymorphic microsatellite loci for the Giant Panda, Ailuropoda melanoleuca . Microsatellite sequences were isolated from three partial genomic libraries of Giant Panda DNA that were enriched for (i) (GT), (ii) (GAA) & (CAA), and (iii) (GATA) repeat sequences. The markers were tested for polymorphism in up to 82 Pandas. Number of alleles at each locus varied between four and 11, and the observed and expected heterozygosities varied between 0.267 and 0.732, and between 0.601 and 0.799, respectively.
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Paternity assignment of Giant Panda by microsatellite genotyping
Yi chuan = Hereditas, 2003Co-Authors: Zhihe Zhang, Anju. Zhang, Fujun Shen, Shan Sun, Victor A. David, Stephen J. O'brienAbstract:We used genotype data across 18 microsatellite loci to establish the paternity of Giant Panda cubs at the Chengdu Zoo and the Chengdu Research Base of Giant Panda Breeding. The results demonstrate that the combined exclusion probability using these 18 microsatellite loci is 0.999921 while confidence is 95 % when the mother is known; if mother is unknown then the exclusion probability is 0.994109, but the confidence of this is less than 80%. Since the mother-offspring relationship is known in captive populations, the results could resolve unknown paternities in the Chengdu ex situ populations. However, to establish accurately the genetic relationships of wild Giant Pandas,more microsatellite loci may be required.
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Pedigree analysis of captive Giant Panda
Yi chuan xue bao = Acta genetica Sinica, 2002Co-Authors: Fujun Shen, Zhihe Zhang, Anju. ZhangAbstract:Abstract Pedigree analysis of captive Giant Panda was conducted by Sparks Ver1.4 Software. The result shows that genetic drift has a strong effect on the loss of genetic diversity. And the dispersal of captive Giant Panda is an acute dangerous factor when all of these small populations would be declined if inbreeding happened. For this reason, all these small populations should be managed as a whole unit to approach the goal for long-term conservation.
Bisong Yue - One of the best experts on this subject based on the ideXlab platform.
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Development of new tetranucleotide microsatellite loci and assessment of genetic variation of Giant Panda in two largest Giant Panda captive breeding populations
Journal of Zoology, 2010Co-Authors: Fujun Shen, Zhihe Zhang, Wenping Zhang, Hou Rong, Bisong YueAbstract:The Giant Panda Ailuropoda melanoleuca is a critically endangered species endemic to China. In order to carry out effective genetic management for the Giant Panda population, sufficient and reliable polymorphic genetic markers are required to provide essential information on the genetic diversity survey of this species. Seven new tetranucleotide microsatellite loci were isolated and characterized in this study and presented here as a tool for evaluating the genetic variation of Giant Pandas in the world's two largest captive populations (Chengdu Research Base of Giant Panda Breeding, Sichuan Province and the China Research and Conservation Center for the Giant Panda in Wolong, Sichuan Province). A total of 45 alleles were identified from these seven new microsatellite loci on the basis of 48 Giant Panda individuals, including 19 private alleles (six from the Chengdu population, 13 from the Wolong population) and 26 shared alleles. The average number of alleles, the average allelic richness the and mean observed heterozygosity were 4.6, 4.367 and 0.649, respectively in the Chengdu population and 5.6, 5.697 and 0.675 in the Wolong population, suggesting that the Chengdu population has a much lower allelic diversity than the Wolong population. Thus, we proposed a better strategy for the captive breeding of Giant Pandas.
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Interferon-γ of the Giant Panda (Ailuropoda melanoleuca): Complementary DNA Cloning, Expression, and Phylogenetic Analysis
DNA and cell biology, 2010Co-Authors: Yaqiong Tao, Bo Zeng, Bisong Yue, Dong Yang, Fangdong ZouAbstract:Interferon-γ (IFN-γ) is the only member of type II IFN and is vital in the regulation of immune and inflammatory responses. Herein we report the cloning, expression, and sequence analysis of IFN-γ from the Giant Panda (Ailuropoda melanoleuca). The open reading frame of this gene is 501 base pair in length and encodes a polypeptide consisting of 166 amino acids. All conserved N-linked glycosylation sites and cysteine residues among carnivores were found in the predicted amino acid sequence of the Giant Panda. Recombinant Giant Panda IFN-γ with a V5 epitope and polyhistidine tag was expressed in HEK293 host cells and confirmed by Western blotting. Phylogenetic analysis of mammalian IFN-γ–coding sequences indicated that the Giant Panda IFN-γ was closest to that of carnivores, then to ungulates and dolphin, and shared a distant relationship with mouse and human. These results represent a first step into the study of IFN-γ in Giant Panda.
Stephen J. Kemp - One of the best experts on this subject based on the ideXlab platform.
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Di-, tri- and tetranucleotide microsatellite loci for the Giant Panda, Ailuropoda melanoleuca
Molecular Ecology Notes, 2007Co-Authors: Fujun Shen, Anju. Zhang, Stephanie Sanderson, Phillip C. Watts, Wei He, Zhihe Zhang, Stephen J. KempAbstract:We describe 10 polymorphic microsatellite loci for the Giant Panda, Ailuropoda melanoleuca . Microsatellite sequences were isolated from three partial genomic libraries of Giant Panda DNA that were enriched for (i) (GT), (ii) (GAA) & (CAA), and (iii) (GATA) repeat sequences. The markers were tested for polymorphism in up to 82 Pandas. Number of alleles at each locus varied between four and 11, and the observed and expected heterozygosities varied between 0.267 and 0.732, and between 0.601 and 0.799, respectively.