The Experts below are selected from a list of 360 Experts worldwide ranked by ideXlab platform
Savitri Sharma - One of the best experts on this subject based on the ideXlab platform.
-
diagnosis of herpes simplex virus 1 keratitis using Giemsa Stain immunofluorescence assay and polymerase chain reaction assay on corneal scrapings
British Journal of Ophthalmology, 2004Co-Authors: S Farhatullah, S Kaza, Sreedharan Athmanathan, Prashant Garg, Sesha B Reddy, Savitri SharmaAbstract:Aims: To evaluate three tests used routinely for the diagnosis of herpes simplex virus (HSV) keratitis. Methods: Corneal scrapings from 28 patients with clinically typical dendritic corneal ulcer suggestive of HSV keratitis, and 30 patients with clinically non-viral corneal ulcers, were tested by (i) Giemsa Stain for multinucleated giant cells, (ii) immunofluorescence assay (IFA) for HSV-1 antigen, and (iii) polymerase chain reaction (PCR) for HSV-1 DNA, by investigators masked to clinical diagnosis. The control subjects were also investigated by smears and cultures for bacteria, fungus, and Acanthamoeba. Results: The specificity and positive predictive values of all three tests for the diagnosis of HSV keratitis were between 95–100%. The sensitivity of IFA and PCR was 78.6% and 81.2%, respectively, and the difference was not significant; however, their sensitivity and negative predictive value were significantly higher than Giemsa Stain. Conclusions: While a combination of IFA and PCR constitute the choice of tests in clinically suspected cases of HSV keratitis, multinucleated giant cells in Giemsa Stain can pre-empt testing by IFA and PCR in otherwise atypical cases of HSV keratitis.
-
diagnosis of herpes simplex virus 1 keratitis comparison of Giemsa Stain immunofluorescence assay and polymerase chain reaction
Current Eye Research, 2004Co-Authors: Shaheen Subhan, Sesha B Reddy, Roby J Jose, Aparna Duggirala, R Hari, P V Krishna, Savitri SharmaAbstract:Purpose. To evaluate three different diagnostic tests against the gold standard of viral isolation, in the diagnosis of HSV1 keratitis. Methods. Corneal scrapings from 170 patients with clinically suspected HSV keratitis were tested by; 1) Giemsa Staining procedure for the presence of multinucleated giant cells and lymphocytes, 2) immunofluorescence assay for HSV-1 antigen, 3) polymerase chain reaction (PCR) for HSV-1 DNA and 4) virus isolation by shell vial culture in SIRC (Rabbit corneal epithelial cell line). The results of the former three tests were compared among 14 cases that were culture positive and 156 cases that were culture negative for HSV-1. Results. The sensitivity of PCR was 100%, while IFA and Giemsa had sensitivities of 85.7% and 57.1% respectively. The specificity of PCR, IFA and Giemsa were found to be 67.9%, 85.3% and 85.9% respectively. Conclusions. In the present study, a combination of PCR and immunofluorescence assay appears to be the most suitable choice of tests for diagnosis of HSV-1 keratitis, while detection of MNGC by Giemsa Staining procedure may give us a presumptive diagnosis of suspected viral infection.
S Farhatullah - One of the best experts on this subject based on the ideXlab platform.
-
diagnosis of herpes simplex virus 1 keratitis using Giemsa Stain immunofluorescence assay and polymerase chain reaction assay on corneal scrapings
British Journal of Ophthalmology, 2004Co-Authors: S Farhatullah, S Kaza, Sreedharan Athmanathan, Prashant Garg, Sesha B Reddy, Savitri SharmaAbstract:Aims: To evaluate three tests used routinely for the diagnosis of herpes simplex virus (HSV) keratitis. Methods: Corneal scrapings from 28 patients with clinically typical dendritic corneal ulcer suggestive of HSV keratitis, and 30 patients with clinically non-viral corneal ulcers, were tested by (i) Giemsa Stain for multinucleated giant cells, (ii) immunofluorescence assay (IFA) for HSV-1 antigen, and (iii) polymerase chain reaction (PCR) for HSV-1 DNA, by investigators masked to clinical diagnosis. The control subjects were also investigated by smears and cultures for bacteria, fungus, and Acanthamoeba. Results: The specificity and positive predictive values of all three tests for the diagnosis of HSV keratitis were between 95–100%. The sensitivity of IFA and PCR was 78.6% and 81.2%, respectively, and the difference was not significant; however, their sensitivity and negative predictive value were significantly higher than Giemsa Stain. Conclusions: While a combination of IFA and PCR constitute the choice of tests in clinically suspected cases of HSV keratitis, multinucleated giant cells in Giemsa Stain can pre-empt testing by IFA and PCR in otherwise atypical cases of HSV keratitis.
Sesha B Reddy - One of the best experts on this subject based on the ideXlab platform.
-
diagnosis of herpes simplex virus 1 keratitis using Giemsa Stain immunofluorescence assay and polymerase chain reaction assay on corneal scrapings
British Journal of Ophthalmology, 2004Co-Authors: S Farhatullah, S Kaza, Sreedharan Athmanathan, Prashant Garg, Sesha B Reddy, Savitri SharmaAbstract:Aims: To evaluate three tests used routinely for the diagnosis of herpes simplex virus (HSV) keratitis. Methods: Corneal scrapings from 28 patients with clinically typical dendritic corneal ulcer suggestive of HSV keratitis, and 30 patients with clinically non-viral corneal ulcers, were tested by (i) Giemsa Stain for multinucleated giant cells, (ii) immunofluorescence assay (IFA) for HSV-1 antigen, and (iii) polymerase chain reaction (PCR) for HSV-1 DNA, by investigators masked to clinical diagnosis. The control subjects were also investigated by smears and cultures for bacteria, fungus, and Acanthamoeba. Results: The specificity and positive predictive values of all three tests for the diagnosis of HSV keratitis were between 95–100%. The sensitivity of IFA and PCR was 78.6% and 81.2%, respectively, and the difference was not significant; however, their sensitivity and negative predictive value were significantly higher than Giemsa Stain. Conclusions: While a combination of IFA and PCR constitute the choice of tests in clinically suspected cases of HSV keratitis, multinucleated giant cells in Giemsa Stain can pre-empt testing by IFA and PCR in otherwise atypical cases of HSV keratitis.
-
diagnosis of herpes simplex virus 1 keratitis comparison of Giemsa Stain immunofluorescence assay and polymerase chain reaction
Current Eye Research, 2004Co-Authors: Shaheen Subhan, Sesha B Reddy, Roby J Jose, Aparna Duggirala, R Hari, P V Krishna, Savitri SharmaAbstract:Purpose. To evaluate three different diagnostic tests against the gold standard of viral isolation, in the diagnosis of HSV1 keratitis. Methods. Corneal scrapings from 170 patients with clinically suspected HSV keratitis were tested by; 1) Giemsa Staining procedure for the presence of multinucleated giant cells and lymphocytes, 2) immunofluorescence assay for HSV-1 antigen, 3) polymerase chain reaction (PCR) for HSV-1 DNA and 4) virus isolation by shell vial culture in SIRC (Rabbit corneal epithelial cell line). The results of the former three tests were compared among 14 cases that were culture positive and 156 cases that were culture negative for HSV-1. Results. The sensitivity of PCR was 100%, while IFA and Giemsa had sensitivities of 85.7% and 57.1% respectively. The specificity of PCR, IFA and Giemsa were found to be 67.9%, 85.3% and 85.9% respectively. Conclusions. In the present study, a combination of PCR and immunofluorescence assay appears to be the most suitable choice of tests for diagnosis of HSV-1 keratitis, while detection of MNGC by Giemsa Staining procedure may give us a presumptive diagnosis of suspected viral infection.
Prashant Garg - One of the best experts on this subject based on the ideXlab platform.
-
diagnosis of herpes simplex virus 1 keratitis using Giemsa Stain immunofluorescence assay and polymerase chain reaction assay on corneal scrapings
British Journal of Ophthalmology, 2004Co-Authors: S Farhatullah, S Kaza, Sreedharan Athmanathan, Prashant Garg, Sesha B Reddy, Savitri SharmaAbstract:Aims: To evaluate three tests used routinely for the diagnosis of herpes simplex virus (HSV) keratitis. Methods: Corneal scrapings from 28 patients with clinically typical dendritic corneal ulcer suggestive of HSV keratitis, and 30 patients with clinically non-viral corneal ulcers, were tested by (i) Giemsa Stain for multinucleated giant cells, (ii) immunofluorescence assay (IFA) for HSV-1 antigen, and (iii) polymerase chain reaction (PCR) for HSV-1 DNA, by investigators masked to clinical diagnosis. The control subjects were also investigated by smears and cultures for bacteria, fungus, and Acanthamoeba. Results: The specificity and positive predictive values of all three tests for the diagnosis of HSV keratitis were between 95–100%. The sensitivity of IFA and PCR was 78.6% and 81.2%, respectively, and the difference was not significant; however, their sensitivity and negative predictive value were significantly higher than Giemsa Stain. Conclusions: While a combination of IFA and PCR constitute the choice of tests in clinically suspected cases of HSV keratitis, multinucleated giant cells in Giemsa Stain can pre-empt testing by IFA and PCR in otherwise atypical cases of HSV keratitis.
Sreedharan Athmanathan - One of the best experts on this subject based on the ideXlab platform.
-
diagnosis of herpes simplex virus 1 keratitis using Giemsa Stain immunofluorescence assay and polymerase chain reaction assay on corneal scrapings
British Journal of Ophthalmology, 2004Co-Authors: S Farhatullah, S Kaza, Sreedharan Athmanathan, Prashant Garg, Sesha B Reddy, Savitri SharmaAbstract:Aims: To evaluate three tests used routinely for the diagnosis of herpes simplex virus (HSV) keratitis. Methods: Corneal scrapings from 28 patients with clinically typical dendritic corneal ulcer suggestive of HSV keratitis, and 30 patients with clinically non-viral corneal ulcers, were tested by (i) Giemsa Stain for multinucleated giant cells, (ii) immunofluorescence assay (IFA) for HSV-1 antigen, and (iii) polymerase chain reaction (PCR) for HSV-1 DNA, by investigators masked to clinical diagnosis. The control subjects were also investigated by smears and cultures for bacteria, fungus, and Acanthamoeba. Results: The specificity and positive predictive values of all three tests for the diagnosis of HSV keratitis were between 95–100%. The sensitivity of IFA and PCR was 78.6% and 81.2%, respectively, and the difference was not significant; however, their sensitivity and negative predictive value were significantly higher than Giemsa Stain. Conclusions: While a combination of IFA and PCR constitute the choice of tests in clinically suspected cases of HSV keratitis, multinucleated giant cells in Giemsa Stain can pre-empt testing by IFA and PCR in otherwise atypical cases of HSV keratitis.