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Steven F Perry - One of the best experts on this subject based on the ideXlab platform.
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morphometric partitioning of the respiratory surface area and diffusion capacity of the Gills and swim bladder in juvenile amazonian air breathing fish arapaima gigas
Micron, 2012Co-Authors: M N Fernandes, Andre Luis Da Cruz, Oscar Tadeu Ferreira Da Costa, Steven F PerryAbstract:a b s t r a c t The Gills and the respiratory swim bladders of juvenile specimens (mean body mass 100 g) of the basal teleost Arapaima gigas (Cuvier 1829) were evaluated using stereological methods in vertical sections. The surface areas, harmonic mean barrier thicknesses and morphometric diffusing capacities for oxygen and carbon dioxide were estimated. The average respiratory surface area of the swim bladder (2173 cm2 kg−1) exceeded that of the Gills (780 cm 2 kg −1 ) by a factor of 2.79. Due to the extremely thin air-blood barrier in the swim bladder (harmonic mean 0.22 m) and the much thicker water-blood barrier of the Gills (9.61 m), the morphometric diffusing capacity for oxygen and carbon dioxide was 88 times greater in the swim bladder than in the Gills. These data clearly indicate the importance of the swim bladder, even in juvenile A. gigas that still engage in aquatic respiration. Because of the much greater diffusion constant of CO2 than O2 in water, the Gills also remain important for CO2 release. © 2012 Elsevier Ltd. All rights reserved.
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morphometric partitioning of the respiratory surface area and diffusion capacity of the Gills and swim bladder in juvenile amazonian air breathing fish arapaima gigas
Micron, 2012Co-Authors: M N Fernandes, Andre Luis Da Cruz, Oscar Tadeu Ferreira Da Costa, Steven F PerryAbstract:a b s t r a c t The Gills and the respiratory swim bladders of juvenile specimens (mean body mass 100 g) of the basal teleost Arapaima gigas (Cuvier 1829) were evaluated using stereological methods in vertical sections. The surface areas, harmonic mean barrier thicknesses and morphometric diffusing capacities for oxygen and carbon dioxide were estimated. The average respiratory surface area of the swim bladder (2173 cm2 kg−1) exceeded that of the Gills (780 cm 2 kg −1 ) by a factor of 2.79. Due to the extremely thin air-blood barrier in the swim bladder (harmonic mean 0.22 m) and the much thicker water-blood barrier of the Gills (9.61 m), the morphometric diffusing capacity for oxygen and carbon dioxide was 88 times greater in the swim bladder than in the Gills. These data clearly indicate the importance of the swim bladder, even in juvenile A. gigas that still engage in aquatic respiration. Because of the much greater diffusion constant of CO2 than O2 in water, the Gills also remain important for CO2 release. © 2012 Elsevier Ltd. All rights reserved.
Tsunghan Lee - One of the best experts on this subject based on the ideXlab platform.
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the effect of environmental salinity on the protein expression of na k atpase na k 2cl cotransporter cystic fibrosis transmembrane conductance regulator anion exchanger 1 and chloride channel 3 in Gills of a euryhaline teleost tetraodon nigroviridis
Comparative Biochemistry and Physiology A-molecular & Integrative Physiology, 2007Co-Authors: Chenghao Tang, Tsunghan LeeAbstract:Abstract Chloride transport mechanisms in the Gills of the estuarine spotted green pufferfish ( Tetraodon nigroviridis ) were investigated. Protein abundance of Na + /K + -ATPase (NKA) and the other four chloride transporters, i.e., Na + /K + /2Cl − cotransporter (NKCC), cystic fibrosis transmembrane conductance regulator (CFTR), Cl − /HCO 3 − anion exchanger 1 (AE1), and chloride channel 3 (CLC-3) in Gills of the seawater- (SW; 35‰) or freshwater (FW)-acclimatized fish were examined by immunoblot analysis. Appropriate negative controls were used to confirm the specificity of the antibodies to the target proteins. The relative protein abundance of NKA was higher (i.e., 2-fold) in Gills of the SW group compared to the FW group. NKCC and CFTR were expressed in Gills of the SW group but not in the FW group. In contrast, the levels of relative protein abundance of branchial AE1 and CLC-3 in the FW group were 23-fold and 2.7-fold higher, respectively, compared to those of the SW group. This study is first of its kind to provide direct in vivo evidence of the protein expression of CLC-3 in teleostean Gills, as well as to examine the simultaneous protein expression of the Cl − transporters, especially AE1 and CLC-3 of FW- and SW-acclimatized teleosts. The differential protein expression of NKA, chloride transporters in Gills of the FW- and SW-acclimatized T. nigroviridis observed in the present study shows their close relationship to the physiological homeostasis (stable blood osmolality), as well as explains the impressive ionoregulatory ability of this euryhaline species in response to salinity challenges.
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the effect of environmental salinity on the protein expression of na k atpase na k 2cl cotransporter cystic fibrosis transmembrane conductance regulator anion exchanger 1 and chloride channel 3 in Gills of a euryhaline teleost tetraodon nigroviridis
Comparative Biochemistry and Physiology A-molecular & Integrative Physiology, 2007Co-Authors: Chenghao Tang, Tsunghan LeeAbstract:Chloride transport mechanisms in the Gills of the estuarine spotted green pufferfish (Tetraodon nigroviridis) were investigated. Protein abundance of Na(+)/K(+)-ATPase (NKA) and the other four chloride transporters, i.e., Na(+)/K(+)/2Cl(-) cotransporter (NKCC), cystic fibrosis transmembrane conductance regulator (CFTR), Cl(-)/HCO(3)(-) anion exchanger 1 (AE1), and chloride channel 3 (CLC-3) in Gills of the seawater- (SW; 35 per thousand) or freshwater (FW)-acclimatized fish were examined by immunoblot analysis. Appropriate negative controls were used to confirm the specificity of the antibodies to the target proteins. The relative protein abundance of NKA was higher (i.e., 2-fold) in Gills of the SW group compared to the FW group. NKCC and CFTR were expressed in Gills of the SW group but not in the FW group. In contrast, the levels of relative protein abundance of branchial AE1 and CLC-3 in the FW group were 23-fold and 2.7-fold higher, respectively, compared to those of the SW group. This study is first of its kind to provide direct in vivo evidence of the protein expression of CLC-3 in teleostean Gills, as well as to examine the simultaneous protein expression of the Cl(-) transporters, especially AE1 and CLC-3 of FW- and SW-acclimatized teleosts. The differential protein expression of NKA, chloride transporters in Gills of the FW- and SW-acclimatized T. nigroviridis observed in the present study shows their close relationship to the physiological homeostasis (stable blood osmolality), as well as explains the impressive ionoregulatory ability of this euryhaline species in response to salinity challenges.
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transmembrane conductance regulator, anion exchanger 1, and chloride channel 3 in Gills of a euryhaline teleost, Tetraodon nigroviridis
2007Co-Authors: C. H. Tang, Tsunghan LeeAbstract:Chloride transport mechanisms in the Gills of the estuarine spotted green pufferfish (Tetraodon nigroviridis) were investigated. Protein abundance of Na + /K + -ATPase (NKA) and the other four chloride transporters, i.e., Na + /K + /2Clcotransporter (NKCC), cystic fibrosis transmembrane conductance regulator (CFTR), Cl � /HCO3 anion exchanger 1 (AE1), and chloride channel 3 (CLC-3) in Gills of the seawater- (SW; 35‰) or freshwater (FW)-acclimatized fish were examined by immunoblot analysis. Appropriate negative controls were used to confirm the specificity of the antibodies to the target proteins. The relative protein abundance of NKA was higher (i.e., 2-fold) in Gills of the SW group compared to the FW group. NKCC and CFTR were expressed in Gills of the SW group but not in the FW group. In contrast, the levels of relative protein abundance of branchial AE1 and CLC-3 in the FW group were 23-fold and 2.7-fold higher, respectively, compared to those of the SW group. This study is first of its kind to provide direct in vivo evidence of the protein expression of CLC-3 in teleostean Gills, as well as to examine the simultaneous protein expression of the Cltransporters, especially AE1 and CLC-3 of FW- and SW-acclimatized teleosts. The differential protein expression of NKA, chloride transporters in Gills of the FW- and SW-acclimatized T. nigroviridis observed in the present study shows their close relationship to the physiological homeostasis (stable blood osmolality), as well as explains the impressive ionoregulatory ability of this euryhaline species in response to salinity challenges.
M N Fernandes - One of the best experts on this subject based on the ideXlab platform.
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morphometric partitioning of the respiratory surface area and diffusion capacity of the Gills and swim bladder in juvenile amazonian air breathing fish arapaima gigas
Micron, 2012Co-Authors: M N Fernandes, Andre Luis Da Cruz, Oscar Tadeu Ferreira Da Costa, Steven F PerryAbstract:a b s t r a c t The Gills and the respiratory swim bladders of juvenile specimens (mean body mass 100 g) of the basal teleost Arapaima gigas (Cuvier 1829) were evaluated using stereological methods in vertical sections. The surface areas, harmonic mean barrier thicknesses and morphometric diffusing capacities for oxygen and carbon dioxide were estimated. The average respiratory surface area of the swim bladder (2173 cm2 kg−1) exceeded that of the Gills (780 cm 2 kg −1 ) by a factor of 2.79. Due to the extremely thin air-blood barrier in the swim bladder (harmonic mean 0.22 m) and the much thicker water-blood barrier of the Gills (9.61 m), the morphometric diffusing capacity for oxygen and carbon dioxide was 88 times greater in the swim bladder than in the Gills. These data clearly indicate the importance of the swim bladder, even in juvenile A. gigas that still engage in aquatic respiration. Because of the much greater diffusion constant of CO2 than O2 in water, the Gills also remain important for CO2 release. © 2012 Elsevier Ltd. All rights reserved.
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morphometric partitioning of the respiratory surface area and diffusion capacity of the Gills and swim bladder in juvenile amazonian air breathing fish arapaima gigas
Micron, 2012Co-Authors: M N Fernandes, Andre Luis Da Cruz, Oscar Tadeu Ferreira Da Costa, Steven F PerryAbstract:a b s t r a c t The Gills and the respiratory swim bladders of juvenile specimens (mean body mass 100 g) of the basal teleost Arapaima gigas (Cuvier 1829) were evaluated using stereological methods in vertical sections. The surface areas, harmonic mean barrier thicknesses and morphometric diffusing capacities for oxygen and carbon dioxide were estimated. The average respiratory surface area of the swim bladder (2173 cm2 kg−1) exceeded that of the Gills (780 cm 2 kg −1 ) by a factor of 2.79. Due to the extremely thin air-blood barrier in the swim bladder (harmonic mean 0.22 m) and the much thicker water-blood barrier of the Gills (9.61 m), the morphometric diffusing capacity for oxygen and carbon dioxide was 88 times greater in the swim bladder than in the Gills. These data clearly indicate the importance of the swim bladder, even in juvenile A. gigas that still engage in aquatic respiration. Because of the much greater diffusion constant of CO2 than O2 in water, the Gills also remain important for CO2 release. © 2012 Elsevier Ltd. All rights reserved.
Katsunori Teranishi - One of the best experts on this subject based on the ideXlab platform.
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bioluminescence and chemiluminescence abilities of trans 3 hydroxyhispidin on the luminous fungus mycena chlorophos
Luminescence, 2018Co-Authors: Katsunori TeranishiAbstract:The fungus Mycena chlorophos emits green light from its pileus Gills but not from its stipes. The chemical mechanisms underlying its bioluminescence are unclear. Trans-3-hydroxyhispidin has been known to be a luminescence substrate for the bioluminescent mycelia of Neonothopanus nambi and N. gardneri. In the present study, the bioluminescence and chemiluminescence abilities of trans-3-hydroxyhispidin on pileus Gills and originally non-bioluminescent stipes of M. chlorophos were demonstrated. Trans-3-hydroxyhispidin induced bioluminescence of living Gills and stipes. The bioluminescence spectra of living Gills and stipes measured after the addition of trans-3-hydroxyhispidin were consistent with the original bioluminescence spectrum of Gills. Frozen-thawed (dead) Gills and stipes maintained trans-3-hydroxyhispidin luminescence activity, and the luminescence-active enzyme (luciferase) was partially purified in the water-insoluble state from both tissues using gel filtration followed by ultracentrifugation. The optimum temperature of the chemiluminescence reactions of trans-3-hydroxyhispidin in the presence of partially purified gill or stipe luciferase was 25°C. The chemiluminescence quantum yields of trans-3-hydroxyhispidin for gill luciferase and stipe luciferase in 20 mM phosphate buffer at 25°C were 0.017 and 0.00096, respectively and the chemiluminescence spectra were almost consistent with the bioluminescence spectrum of living Gills. These results indicate that trans-3-hydroxyhispidin can be a candidate as a substrate for M. chlorophos bioluminescence.
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trans 3 hydroxyhispidin is not an actual bioluminescence substrate in pileus Gills of the luminous fungus mycena chlorophos
Biochemical and Biophysical Research Communications, 2018Co-Authors: Katsunori TeranishiAbstract:Mycena chlorophos is a species of molecular oxygen-dependent bioluminescent fungus, and its pileus Gills emit bright green light. The chemical mechanisms underlying this bioluminescence phenomenon are not yet understood. An enzyme (luciferase) producing light from trans-3-hydroxyhispidin is present in M. chlorophos pileus Gills. However, it is unclear whether trans-3-hydroxyhispidin is an actual bioluminescence substrate (luciferin) in the natural bioluminescence of M. chlorophos. In the present study, this question is resolved. It was clearly demonstrated that the trans-3-hydroxyhispidin analog trans-3-hydroxybisnoryangonin significantly inhibited the artificial luminescence induced by the addition of trans-3-hydroxyhispidin to living pileus Gills but did not inhibit natural bioluminescence in living pileus Gills. This inhibition was due to the reaction of trans-3-hydroxybisnoryangonin with luciferase for trans-3-hydroxyhispidin. Even though trans-4-aminocinnamic acid is known to inhibit natural bioluminescence in living pileus Gills, in the present study, trans-4-aminocinnamic acid did not influence the artificial luminescence via trans-3-hydroxyhispidin in the presence of luciferase for trans-3-hydroxyhispidin. These inconsistencies between the natural bioluminescence and the artificial luminescence of trans-3-hydroxyhispidin indicate that trans-3-hydroxyhispidin is not an actual luciferin in natural bioluminescence. Trans-3,4-dihydroxycinnamic acid is generally known to be an intermediate in trans-3-hydroxyhispidin biosynthesis. The artificial luminescence induced by the addition of trans-3,4-dihydroxycinnamic acid to living pileus Gills was not inhibited by trans-3-hydroxybisnoryangonin. Therefore, trans-3,4-dihydroxycinnamic acid does not contribute to the luminescence involving trans-3-hydroxyhispidin in living pileus Gills.
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second bioluminescence activating component in the luminous fungus mycena chlorophos
Luminescence, 2017Co-Authors: Katsunori TeranishiAbstract:Mycena chlorophos is an oxygen-dependent bioluminescent fungus. The mechanisms underlying its light emission are unknown. A component that increased the bioluminescence intensity of the immature living Gills of M. chlorophos was isolated from mature M. chlorophos Gills and chemically characterized. The bioluminescence-activating component was found to be trans-3,4-dihydroxycinnamic acid and its bioluminescence activation was highly structure-specific. 13 C- and 18 O-labelling studies using the immature living Gills showed that trans-3,4-dihydroxycinnamic acid was synthesized from trans-4-hydroxycinnamic acid in the Gills by hydroxylation with molecular oxygen as well as by the general metabolism, and trans-3,4-dihydroxycinnamic acid did not produce hispidin (detection-limit concentration: 10 pmol/1 g wet gill). Addition of 0.01 mM hispidin to the immature living Gills generated no bioluminescence activation. These results suggested that the prompt bioluminescence activation resulting from addition of trans-3,4-dihydroxycinnamic acid could not be attributed to the generation of hispidin. Copyright © 2016 John Wiley & Sons, Ltd.
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a combination of nadhp and hispidin is not essential for bioluminescence in luminous fungal living Gills of mycena chlorophos
Luminescence, 2017Co-Authors: Katsunori TeranishiAbstract:The chemical mechanisms underlying visible bioluminescence in the fungus Mycena chlorophos are not clear. A combination of dihydronicotinamide adenine dinucleotide phosphate (NADPH) and hispidin, which has been reported to increase the intensity of in vitro luminescence in crude cold-water extracts prepared from the bioluminescent fruiting bodies of M. chlorophos, exhibited potential bioluminescence activation in the early bioluminescence stages, in which the bioluminescence was ultra-weak, for living Gills and luminescence activation for non-bioluminescent Gills, which was collapsed by freezing and subsequent thawing, at all bioluminescence stages. These abilities were not evident in considerably bioluminescent Gills. These abilities were blocked by trans-4-hydroxycinnamic acid and trans-3,4-dihydroxycinnamic acid, which were identified as in vivo bioluminescence-activating components. Original bioluminescence and bioluminescence produced from the addition of trans-4-hydroxycinnamic acid and trans-3,4-dihydroxycinnamic acid in living Gills were almost completely inhibited by 10 mM NaN3, whereas the luminescence produced form the combination of NADPH and hispidin in thawed non-bioluminescent and living Gills at the early weak bioluminescence stages was not inhibited by 10 mM NaN3. Thus, the combination of NADPH and hispidin plays different roles in luminescence systems compared with essential bioluminescence systems, and the combination of NADPH and hispidin was not essential for visible bioluminescence in living Gills.
Chenghao Tang - One of the best experts on this subject based on the ideXlab platform.
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the effect of environmental salinity on the protein expression of na k atpase na k 2cl cotransporter cystic fibrosis transmembrane conductance regulator anion exchanger 1 and chloride channel 3 in Gills of a euryhaline teleost tetraodon nigroviridis
Comparative Biochemistry and Physiology A-molecular & Integrative Physiology, 2007Co-Authors: Chenghao Tang, Tsunghan LeeAbstract:Abstract Chloride transport mechanisms in the Gills of the estuarine spotted green pufferfish ( Tetraodon nigroviridis ) were investigated. Protein abundance of Na + /K + -ATPase (NKA) and the other four chloride transporters, i.e., Na + /K + /2Cl − cotransporter (NKCC), cystic fibrosis transmembrane conductance regulator (CFTR), Cl − /HCO 3 − anion exchanger 1 (AE1), and chloride channel 3 (CLC-3) in Gills of the seawater- (SW; 35‰) or freshwater (FW)-acclimatized fish were examined by immunoblot analysis. Appropriate negative controls were used to confirm the specificity of the antibodies to the target proteins. The relative protein abundance of NKA was higher (i.e., 2-fold) in Gills of the SW group compared to the FW group. NKCC and CFTR were expressed in Gills of the SW group but not in the FW group. In contrast, the levels of relative protein abundance of branchial AE1 and CLC-3 in the FW group were 23-fold and 2.7-fold higher, respectively, compared to those of the SW group. This study is first of its kind to provide direct in vivo evidence of the protein expression of CLC-3 in teleostean Gills, as well as to examine the simultaneous protein expression of the Cl − transporters, especially AE1 and CLC-3 of FW- and SW-acclimatized teleosts. The differential protein expression of NKA, chloride transporters in Gills of the FW- and SW-acclimatized T. nigroviridis observed in the present study shows their close relationship to the physiological homeostasis (stable blood osmolality), as well as explains the impressive ionoregulatory ability of this euryhaline species in response to salinity challenges.
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the effect of environmental salinity on the protein expression of na k atpase na k 2cl cotransporter cystic fibrosis transmembrane conductance regulator anion exchanger 1 and chloride channel 3 in Gills of a euryhaline teleost tetraodon nigroviridis
Comparative Biochemistry and Physiology A-molecular & Integrative Physiology, 2007Co-Authors: Chenghao Tang, Tsunghan LeeAbstract:Chloride transport mechanisms in the Gills of the estuarine spotted green pufferfish (Tetraodon nigroviridis) were investigated. Protein abundance of Na(+)/K(+)-ATPase (NKA) and the other four chloride transporters, i.e., Na(+)/K(+)/2Cl(-) cotransporter (NKCC), cystic fibrosis transmembrane conductance regulator (CFTR), Cl(-)/HCO(3)(-) anion exchanger 1 (AE1), and chloride channel 3 (CLC-3) in Gills of the seawater- (SW; 35 per thousand) or freshwater (FW)-acclimatized fish were examined by immunoblot analysis. Appropriate negative controls were used to confirm the specificity of the antibodies to the target proteins. The relative protein abundance of NKA was higher (i.e., 2-fold) in Gills of the SW group compared to the FW group. NKCC and CFTR were expressed in Gills of the SW group but not in the FW group. In contrast, the levels of relative protein abundance of branchial AE1 and CLC-3 in the FW group were 23-fold and 2.7-fold higher, respectively, compared to those of the SW group. This study is first of its kind to provide direct in vivo evidence of the protein expression of CLC-3 in teleostean Gills, as well as to examine the simultaneous protein expression of the Cl(-) transporters, especially AE1 and CLC-3 of FW- and SW-acclimatized teleosts. The differential protein expression of NKA, chloride transporters in Gills of the FW- and SW-acclimatized T. nigroviridis observed in the present study shows their close relationship to the physiological homeostasis (stable blood osmolality), as well as explains the impressive ionoregulatory ability of this euryhaline species in response to salinity challenges.