The Experts below are selected from a list of 6 Experts worldwide ranked by ideXlab platform

Tubagus Djumhana Atmakusuma - One of the best experts on this subject based on the ideXlab platform.

  • Association of Nucleophosmin-1 (NPM1) Expression with Leucocyte Count, Blast Count, and Gingiva Hypertrophy in Acute Myeloid Leukemia De Novo Patients in General Hospital of Dr M Djamil Padang Indonesia
    Blood, 2019
    Co-Authors: Irza Wahid, Tubagus Djumhana Atmakusuma
    Abstract:

    Acute myeloid leukemia (AML) is a malignant disease derived from hematopoietic system characterized by neoplastic transformation of myeloid progenitor cell, present as excessive proliferation and differentiation alteration leading to inhibition of myeloid maturation. From various studies, incident and prevalence of AML these days is increasing in morbidity and mortality. This make AML known with poor prognosis. Nucleophosmin-1 (NPM-1) was established by WHO revision 2016 as prognostic gene marker in AML. Nucleophosmin-1 gene is mutated in 35-60% of AML cases. Recently, molecular biologic development suggests that prognostic gene examination is needed to reduce morbidity and mortality of AML patients. If myeloid progenitor cell encounters carcinogenic/leukemogenic stresses, mutation of several genes could happen. NPM1 gene is a likely mutated gene and predominantly located in nucleolus. Overexpression of NPM1 will promote activities of tumor suppressors like P53, ARF and MDM2. Interactions of those tumor suppressors promote proliferation, differentiation, cell survival, apoptosis and DNA repair, which give a better prognosis in AML patients. To study about association of NPM1 expression with leucocyte count, blast count, and Gingiva Hypertrophy in AML de novo patients in general hospital of Dr M Djamil Padang, Indonesia. Analytic observational study with cross-sectional approach to 25 adult AML patients from Policlinic and Inpatient Installation of Dr M Djamil Hospital from March to August 2018 who met inclusion and exclusion criteria. Expression of NPM1 was measured with qRT-PCR. We found significant increase of NPM1 expression in AML in the amount of 22.62x107 copies/ml. There was a significant very strong correlation between NPM1 expression and leucocyte count (p<0.05 & r=0.870) and blast count (p<0.05 & r=0.828). There was also significant differences of NPM1 expression in AML patients with Gingival Hypertrophy and without Gingival Hypertrophy (31.3 x 107 copies/ml dan 9.6 x 107 copies/ml). There was a significant very strong correlation between NPM1 expression with leucocyte count and blast count. There was significant increases of NPM1 expression in AML patients with Gingival Hypertrophy. Keywords: acute myeloid leukemia, Nucleophosmin-1, leucocyte, blast, Gingival Hypertrophy Disclosures No relevant conflicts of interest to declare.

Irza Wahid - One of the best experts on this subject based on the ideXlab platform.

  • Association of Nucleophosmin-1 (NPM1) Expression with Leucocyte Count, Blast Count, and Gingiva Hypertrophy in Acute Myeloid Leukemia De Novo Patients in General Hospital of Dr M Djamil Padang Indonesia
    Blood, 2019
    Co-Authors: Irza Wahid, Tubagus Djumhana Atmakusuma
    Abstract:

    Acute myeloid leukemia (AML) is a malignant disease derived from hematopoietic system characterized by neoplastic transformation of myeloid progenitor cell, present as excessive proliferation and differentiation alteration leading to inhibition of myeloid maturation. From various studies, incident and prevalence of AML these days is increasing in morbidity and mortality. This make AML known with poor prognosis. Nucleophosmin-1 (NPM-1) was established by WHO revision 2016 as prognostic gene marker in AML. Nucleophosmin-1 gene is mutated in 35-60% of AML cases. Recently, molecular biologic development suggests that prognostic gene examination is needed to reduce morbidity and mortality of AML patients. If myeloid progenitor cell encounters carcinogenic/leukemogenic stresses, mutation of several genes could happen. NPM1 gene is a likely mutated gene and predominantly located in nucleolus. Overexpression of NPM1 will promote activities of tumor suppressors like P53, ARF and MDM2. Interactions of those tumor suppressors promote proliferation, differentiation, cell survival, apoptosis and DNA repair, which give a better prognosis in AML patients. To study about association of NPM1 expression with leucocyte count, blast count, and Gingiva Hypertrophy in AML de novo patients in general hospital of Dr M Djamil Padang, Indonesia. Analytic observational study with cross-sectional approach to 25 adult AML patients from Policlinic and Inpatient Installation of Dr M Djamil Hospital from March to August 2018 who met inclusion and exclusion criteria. Expression of NPM1 was measured with qRT-PCR. We found significant increase of NPM1 expression in AML in the amount of 22.62x107 copies/ml. There was a significant very strong correlation between NPM1 expression and leucocyte count (p<0.05 & r=0.870) and blast count (p<0.05 & r=0.828). There was also significant differences of NPM1 expression in AML patients with Gingival Hypertrophy and without Gingival Hypertrophy (31.3 x 107 copies/ml dan 9.6 x 107 copies/ml). There was a significant very strong correlation between NPM1 expression with leucocyte count and blast count. There was significant increases of NPM1 expression in AML patients with Gingival Hypertrophy. Keywords: acute myeloid leukemia, Nucleophosmin-1, leucocyte, blast, Gingival Hypertrophy Disclosures No relevant conflicts of interest to declare.

Simona Sica - One of the best experts on this subject based on the ideXlab platform.

  • Peculiar Morphological, Cytochemical, Biochemical and Immunophenotyping Features in One Case of Acute Myeloid Leukaemia at the Onset.
    Blood, 2005
    Co-Authors: Gina Zini, Elena Rossi, Mariagrazia Garzia, Carlo Rumi, Luca Laurenti, Antonella Di Mario, Simona Sica
    Abstract:

    On July 28 th 2005, a 53-year old woman was admitted to our Hematology Dpt. with fever (37.5 C), fatigue, marked Gingiva Hypertrophy, mild hepatomegaly and absence of lymphoadenopaty and splenomegaly. Laboratory tests showed high leukocyte count of 182 x10 9 /L, moderately increased platelet count (438 x10 9 /L), normocytic anemia (10,9 g/dl, MCV 84 fl). Biochemical parameters, including lysozyme, were in the normal range except for high level of lactate dehydrogenase (2524 UI/l). Peripheral blood (PB) smear showed the presence of 94% of blasts, without evident granules. At the cytochemical myeloperoxidase staining blast cells revealed the presence of single or multiple Auer rods, while cytoplasmic granules were positive to the α-naphtyl butyrate esterase. In the bone marrow (BM) aspirate blasts were 80% showing same morphological and cytochemical features as in peripheral blood, while the granulocytic maturating series was 14%. No evidence of morphologically identifiable monocytic series in PB neither in BM. Megakariocytes were slightly increased and there was trilineage dysplasia. Blast immunophenotyping in PB and in BM was as follow: CD45, CD33, CD13, CD7 and HLA-Dr positive, 36% MPO positive and 48% co-express CD11c an CD117. Moreover blasts were CD14, CD19 and CD56 negative. FAB diagnosis was AML-M4. Interphase Fluorescence in situ Hybridization (FISH) for detection of AML1/ETO and bcr/abl gene rearrangements was negative. After treatment with 2 g hydroxyurea and leucapheresis, the WBC count was still high (140 x10 9 /L) while after 1g of aracytin, WBC count decreased to 31000/mm 3. Patient is presently enrolled into the GIMEMA protocol AML-12. This case shows at the onset following abnormalities: - an increased platelet count is a rare evenience in an AML at the onset; - negativity for lysozyme; - absence of CD14; - presence of Auer rods in quite all the blast population, which is a very rare evenience associated with monocytic subtypes of acute myeloblastic leukemia (AML); - atypical homogenous cytochemical pattern of blasts, showing Auer rods MPO positive and granules ANBE positive; - absence of any morphological differentiation of monocytic series. S4479

Gina Zini - One of the best experts on this subject based on the ideXlab platform.

  • Peculiar Morphological, Cytochemical, Biochemical and Immunophenotyping Features in One Case of Acute Myeloid Leukaemia at the Onset.
    Blood, 2005
    Co-Authors: Gina Zini, Elena Rossi, Mariagrazia Garzia, Carlo Rumi, Luca Laurenti, Antonella Di Mario, Simona Sica
    Abstract:

    On July 28 th 2005, a 53-year old woman was admitted to our Hematology Dpt. with fever (37.5 C), fatigue, marked Gingiva Hypertrophy, mild hepatomegaly and absence of lymphoadenopaty and splenomegaly. Laboratory tests showed high leukocyte count of 182 x10 9 /L, moderately increased platelet count (438 x10 9 /L), normocytic anemia (10,9 g/dl, MCV 84 fl). Biochemical parameters, including lysozyme, were in the normal range except for high level of lactate dehydrogenase (2524 UI/l). Peripheral blood (PB) smear showed the presence of 94% of blasts, without evident granules. At the cytochemical myeloperoxidase staining blast cells revealed the presence of single or multiple Auer rods, while cytoplasmic granules were positive to the α-naphtyl butyrate esterase. In the bone marrow (BM) aspirate blasts were 80% showing same morphological and cytochemical features as in peripheral blood, while the granulocytic maturating series was 14%. No evidence of morphologically identifiable monocytic series in PB neither in BM. Megakariocytes were slightly increased and there was trilineage dysplasia. Blast immunophenotyping in PB and in BM was as follow: CD45, CD33, CD13, CD7 and HLA-Dr positive, 36% MPO positive and 48% co-express CD11c an CD117. Moreover blasts were CD14, CD19 and CD56 negative. FAB diagnosis was AML-M4. Interphase Fluorescence in situ Hybridization (FISH) for detection of AML1/ETO and bcr/abl gene rearrangements was negative. After treatment with 2 g hydroxyurea and leucapheresis, the WBC count was still high (140 x10 9 /L) while after 1g of aracytin, WBC count decreased to 31000/mm 3. Patient is presently enrolled into the GIMEMA protocol AML-12. This case shows at the onset following abnormalities: - an increased platelet count is a rare evenience in an AML at the onset; - negativity for lysozyme; - absence of CD14; - presence of Auer rods in quite all the blast population, which is a very rare evenience associated with monocytic subtypes of acute myeloblastic leukemia (AML); - atypical homogenous cytochemical pattern of blasts, showing Auer rods MPO positive and granules ANBE positive; - absence of any morphological differentiation of monocytic series. S4479

Elena Rossi - One of the best experts on this subject based on the ideXlab platform.

  • Peculiar Morphological, Cytochemical, Biochemical and Immunophenotyping Features in One Case of Acute Myeloid Leukaemia at the Onset.
    Blood, 2005
    Co-Authors: Gina Zini, Elena Rossi, Mariagrazia Garzia, Carlo Rumi, Luca Laurenti, Antonella Di Mario, Simona Sica
    Abstract:

    On July 28 th 2005, a 53-year old woman was admitted to our Hematology Dpt. with fever (37.5 C), fatigue, marked Gingiva Hypertrophy, mild hepatomegaly and absence of lymphoadenopaty and splenomegaly. Laboratory tests showed high leukocyte count of 182 x10 9 /L, moderately increased platelet count (438 x10 9 /L), normocytic anemia (10,9 g/dl, MCV 84 fl). Biochemical parameters, including lysozyme, were in the normal range except for high level of lactate dehydrogenase (2524 UI/l). Peripheral blood (PB) smear showed the presence of 94% of blasts, without evident granules. At the cytochemical myeloperoxidase staining blast cells revealed the presence of single or multiple Auer rods, while cytoplasmic granules were positive to the α-naphtyl butyrate esterase. In the bone marrow (BM) aspirate blasts were 80% showing same morphological and cytochemical features as in peripheral blood, while the granulocytic maturating series was 14%. No evidence of morphologically identifiable monocytic series in PB neither in BM. Megakariocytes were slightly increased and there was trilineage dysplasia. Blast immunophenotyping in PB and in BM was as follow: CD45, CD33, CD13, CD7 and HLA-Dr positive, 36% MPO positive and 48% co-express CD11c an CD117. Moreover blasts were CD14, CD19 and CD56 negative. FAB diagnosis was AML-M4. Interphase Fluorescence in situ Hybridization (FISH) for detection of AML1/ETO and bcr/abl gene rearrangements was negative. After treatment with 2 g hydroxyurea and leucapheresis, the WBC count was still high (140 x10 9 /L) while after 1g of aracytin, WBC count decreased to 31000/mm 3. Patient is presently enrolled into the GIMEMA protocol AML-12. This case shows at the onset following abnormalities: - an increased platelet count is a rare evenience in an AML at the onset; - negativity for lysozyme; - absence of CD14; - presence of Auer rods in quite all the blast population, which is a very rare evenience associated with monocytic subtypes of acute myeloblastic leukemia (AML); - atypical homogenous cytochemical pattern of blasts, showing Auer rods MPO positive and granules ANBE positive; - absence of any morphological differentiation of monocytic series. S4479