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Roger B. Johnson - One of the best experts on this subject based on the ideXlab platform.
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relationship between Gingival angiopoietin 1 concentrations and depth of the adjacent Gingival sulcus
Journal of Periodontology, 2009Co-Authors: Reid S Lester, J.l. Bain, Francis G. Serio, Bradley D Harrelson, Roger B. JohnsonAbstract:Background: The purpose of this study was to assess concentrations of angiopoietin (Ang)-1 at various stages of Gingival inflammation. Ang-1 is an anti-inflammatory mediator present in various inflammatory diseases. However, its presence during the pathogenesis of Gingival inflammation has not been established in vivo.Methods: Gingiva was obtained from 110 human donors before extraction of the adjacent teeth. The tissue was grouped based on adjacent probing depth and bleeding on probing (BOP). Gingiva adjacent to a ≤3-mm sulcus without BOP was classified as “normal” (N); Gingiva adjacent to a 3-mm sulcus with BOP was classified as “diseased, slight” (DS); Gingiva adjacent to a 4- to 6-mm sulcus featuring BOP was classified as “diseased, moderate” (DM); and Gingiva adjacent to >6-mm sulci was classified as “diseased, severe” (DSev). Tissues were solublized, and concentrations of interleukin (IL)-1β and -6, tumor necrosis factor (TNF)-α, endothelin (ET)-1, Ang-1, vascular cell adhesion molecule (VCAM)-1, an...
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Relationship between the Gingival sulcus depth and interleukin-1 isoform concentrations within the adjacent Gingival tissue.
Journal of Periodontal Research, 2008Co-Authors: S. R. Lester, J.l. Bain, Francis G. Serio, Roger B. JohnsonAbstract:Background and Objective: While there is substantial information concerning the concentrations of interleukin-1 isoforms within Gingival crevicular fluid, there is little information concerning their concentrations within either normal or diseased Gingival tissues. Therefore, the aim of this study was to evaluate the relationship between the concentrations of Gingival interleukin-1 isoforms and the adjacent sulcular depth. Material and Methods: Interdental Gingival papillae were excised and grouped based on adjacent pocket depth and the presence of bleeding on probing. Gingiva adjacent to a sulcus of ≤ 3 mm without bleeding on probing were classified as ‘normal’; Gingiva adjacent to a 3-mm sulcus with bleeding on probing were classified as ‘diseased-slight’; Gingiva adjacent to a 4–6-mm sulcus featuring bleeding on probing were classified as ‘diseased-moderate’; and Gingiva adjacent to a sulcus of > 6 mm featuring bleeding on probing were classified as ‘diseased-severe’. Tissues were solublized and the concentrations of interleukin-1β, interleukin-1α, interleukin-1 receptor antagonist and interleukin-6 were assessed by enzyme-linked immunosorbent assay. Data were compared by factorial analysis of variance, the post-hoc Tukey test and the Pearson’s correlation test. Results: Gingival concentrations of interleukin-6, interleukin-1 receptor antagonist, interleukin-1α- and interleukin-1β were significantly greater at diseased-severe sites than at normal, diseased-slight, or diseased-moderate sites (p
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Interleukin-11 and IL-17 and the pathogenesis of periodontal disease.
Journal of Periodontology, 2004Co-Authors: Roger B. Johnson, N. Wood, Francis G. SerioAbstract:Background: Interleukin (IL)-11 and IL-17 are cytokines that modulate the inflammatory process and have not been assessed within normal or inflamed Gingival tissues. Our purpose was to compare concentrations of human IL-11 and IL-17 within healthy and diseased human Gingiva to determine their possible role in the initiation or progression of periodontal diseases. Methods: Biopsies from healthy (non-hemorrhagic Gingiva adjacent to a ≤3 mm Gingival sulcus) and diseased Gingiva (hemorrhagic Gingiva adjacent to a ≥3 mm periodontal pocket) were studied. IL-11, IL-17, RANTES, and IL-6 concentrations were assessed within solubilized Gingival biopsies by enzyme-linked immunosorbent assay. Data were compared by factorial analysis of variance and a post-hoc Tukey honestly significant difference (HSD) test. Regression analysis and partial correlation analysis (adjusted for sample weight) were also used to determine correlations between the variables. Results: Interleukin-11 concentrations were highest within Gingiva...
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Leptin Within Healthy and Diseased Human Gingiva
Journal of periodontology, 2001Co-Authors: Roger B. Johnson, Francis G. SerioAbstract:Background: Plasma leptin concentrations are reported to be elevated in patients with inflammatory diseases. There is no consensus concerning the biological mechanism for this phenomenon. To date, tissue leptin concentrations have not been assessed within normal or inflamed Gingiva. The purpose of this study was to assess concentrations of human leptin within healthy and diseased Gingiva to define its possible role in periodontal disease progression. Methods: Healthy (non-hemorrhagic Gingiva adjacent to ≤3 mm Gingival sulcus) and inflamed Gingiva (hemorrhagic Gingiva adjacent to a ≥3 mm periodontal pocket) were studied. Leptin, vascular endothelial growth factor (VEGF; to assess potential vascular expansion), and interleukin-6 (IL-6; to assess periodontal disease activity and severity) concentrations were assessed within solubilized Gingival biopsies by enzyme-linked immunosorbent assay. Data were grouped by sulcular depth and compared by factorial analysis of variance, regression analysis, and Scheffe co...
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vascular endothelial growth factors and progression of periodontal diseases
Journal of Periodontology, 1999Co-Authors: Roger B. Johnson, Francis G. SerioAbstract:Background: Tissues become hemorrhagic and edematous coincident to periodontal diseases; however, there is little information concerning the biologic mechanisms which may produce these changes. Vascular endothelial growth factor (VEGF) is a macromolecule which enhances blood vessel growth and permeability. However, there is no information concerning Gingival VEGF concentrations within normal or diseased Gingiva. The purpose of this study was to assess changes in Gingival concentrations of VEGF during initiation and progression of periodontal diseases and compare them to changes in the number of blood vessel profiles and concentration of recognized markers of periodontal disease severity (interleukin- 6[IL-6]). Methods: Normal (non-hemorrhagic Gingiva adjacent to a ≤3 mm Gingival sulcus) and inflamed Gingiva (hemorrhagic Gingiva adjacent to a ≤3 mm, 4 to 6 mm, or >6 mm periodontal pocket) were studied. VEGF and IL-6 concentrations were assessed by ELlSA and the number of blood vessels determined by histomo...
Francis G. Serio - One of the best experts on this subject based on the ideXlab platform.
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relationship between Gingival angiopoietin 1 concentrations and depth of the adjacent Gingival sulcus
Journal of Periodontology, 2009Co-Authors: Reid S Lester, J.l. Bain, Francis G. Serio, Bradley D Harrelson, Roger B. JohnsonAbstract:Background: The purpose of this study was to assess concentrations of angiopoietin (Ang)-1 at various stages of Gingival inflammation. Ang-1 is an anti-inflammatory mediator present in various inflammatory diseases. However, its presence during the pathogenesis of Gingival inflammation has not been established in vivo.Methods: Gingiva was obtained from 110 human donors before extraction of the adjacent teeth. The tissue was grouped based on adjacent probing depth and bleeding on probing (BOP). Gingiva adjacent to a ≤3-mm sulcus without BOP was classified as “normal” (N); Gingiva adjacent to a 3-mm sulcus with BOP was classified as “diseased, slight” (DS); Gingiva adjacent to a 4- to 6-mm sulcus featuring BOP was classified as “diseased, moderate” (DM); and Gingiva adjacent to >6-mm sulci was classified as “diseased, severe” (DSev). Tissues were solublized, and concentrations of interleukin (IL)-1β and -6, tumor necrosis factor (TNF)-α, endothelin (ET)-1, Ang-1, vascular cell adhesion molecule (VCAM)-1, an...
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Relationship between the Gingival sulcus depth and interleukin-1 isoform concentrations within the adjacent Gingival tissue.
Journal of Periodontal Research, 2008Co-Authors: S. R. Lester, J.l. Bain, Francis G. Serio, Roger B. JohnsonAbstract:Background and Objective: While there is substantial information concerning the concentrations of interleukin-1 isoforms within Gingival crevicular fluid, there is little information concerning their concentrations within either normal or diseased Gingival tissues. Therefore, the aim of this study was to evaluate the relationship between the concentrations of Gingival interleukin-1 isoforms and the adjacent sulcular depth. Material and Methods: Interdental Gingival papillae were excised and grouped based on adjacent pocket depth and the presence of bleeding on probing. Gingiva adjacent to a sulcus of ≤ 3 mm without bleeding on probing were classified as ‘normal’; Gingiva adjacent to a 3-mm sulcus with bleeding on probing were classified as ‘diseased-slight’; Gingiva adjacent to a 4–6-mm sulcus featuring bleeding on probing were classified as ‘diseased-moderate’; and Gingiva adjacent to a sulcus of > 6 mm featuring bleeding on probing were classified as ‘diseased-severe’. Tissues were solublized and the concentrations of interleukin-1β, interleukin-1α, interleukin-1 receptor antagonist and interleukin-6 were assessed by enzyme-linked immunosorbent assay. Data were compared by factorial analysis of variance, the post-hoc Tukey test and the Pearson’s correlation test. Results: Gingival concentrations of interleukin-6, interleukin-1 receptor antagonist, interleukin-1α- and interleukin-1β were significantly greater at diseased-severe sites than at normal, diseased-slight, or diseased-moderate sites (p
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Interleukin-11 and IL-17 and the pathogenesis of periodontal disease.
Journal of Periodontology, 2004Co-Authors: Roger B. Johnson, N. Wood, Francis G. SerioAbstract:Background: Interleukin (IL)-11 and IL-17 are cytokines that modulate the inflammatory process and have not been assessed within normal or inflamed Gingival tissues. Our purpose was to compare concentrations of human IL-11 and IL-17 within healthy and diseased human Gingiva to determine their possible role in the initiation or progression of periodontal diseases. Methods: Biopsies from healthy (non-hemorrhagic Gingiva adjacent to a ≤3 mm Gingival sulcus) and diseased Gingiva (hemorrhagic Gingiva adjacent to a ≥3 mm periodontal pocket) were studied. IL-11, IL-17, RANTES, and IL-6 concentrations were assessed within solubilized Gingival biopsies by enzyme-linked immunosorbent assay. Data were compared by factorial analysis of variance and a post-hoc Tukey honestly significant difference (HSD) test. Regression analysis and partial correlation analysis (adjusted for sample weight) were also used to determine correlations between the variables. Results: Interleukin-11 concentrations were highest within Gingiva...
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Leptin Within Healthy and Diseased Human Gingiva
Journal of periodontology, 2001Co-Authors: Roger B. Johnson, Francis G. SerioAbstract:Background: Plasma leptin concentrations are reported to be elevated in patients with inflammatory diseases. There is no consensus concerning the biological mechanism for this phenomenon. To date, tissue leptin concentrations have not been assessed within normal or inflamed Gingiva. The purpose of this study was to assess concentrations of human leptin within healthy and diseased Gingiva to define its possible role in periodontal disease progression. Methods: Healthy (non-hemorrhagic Gingiva adjacent to ≤3 mm Gingival sulcus) and inflamed Gingiva (hemorrhagic Gingiva adjacent to a ≥3 mm periodontal pocket) were studied. Leptin, vascular endothelial growth factor (VEGF; to assess potential vascular expansion), and interleukin-6 (IL-6; to assess periodontal disease activity and severity) concentrations were assessed within solubilized Gingival biopsies by enzyme-linked immunosorbent assay. Data were grouped by sulcular depth and compared by factorial analysis of variance, regression analysis, and Scheffe co...
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vascular endothelial growth factors and progression of periodontal diseases
Journal of Periodontology, 1999Co-Authors: Roger B. Johnson, Francis G. SerioAbstract:Background: Tissues become hemorrhagic and edematous coincident to periodontal diseases; however, there is little information concerning the biologic mechanisms which may produce these changes. Vascular endothelial growth factor (VEGF) is a macromolecule which enhances blood vessel growth and permeability. However, there is no information concerning Gingival VEGF concentrations within normal or diseased Gingiva. The purpose of this study was to assess changes in Gingival concentrations of VEGF during initiation and progression of periodontal diseases and compare them to changes in the number of blood vessel profiles and concentration of recognized markers of periodontal disease severity (interleukin- 6[IL-6]). Methods: Normal (non-hemorrhagic Gingiva adjacent to a ≤3 mm Gingival sulcus) and inflamed Gingiva (hemorrhagic Gingiva adjacent to a ≤3 mm, 4 to 6 mm, or >6 mm periodontal pocket) were studied. VEGF and IL-6 concentrations were assessed by ELlSA and the number of blood vessels determined by histomo...
Shin Nieh - One of the best experts on this subject based on the ideXlab platform.
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A stereomicroscopic and immunohistochemical study of vasculature in Gingiva bleeding after probing.
Journal of Periodontology, 1992Co-Authors: Earl Fu, Shin NiehAbstract:This study investigated the stereomicroscopic appearance of Gingival sulcular surfaces and the histologic alterations of subepithelial vasculature of Gingiva bleeding after probing. Sixty-four pairs of Gingival biopsies from 49 patients were obtained and the results of stereomicroscopically visible vasculature were observed in half of the specimens. The number of biopsies showing that the vasculature of bleeding Gingiva was significantly greater than those of non-bleeding Gingiva. The immunohistochemical marker UEA-1 was used to identify endothelial cells on the vascular walls of 34 Gingival biopsies from 15 patients. Following stereomicroscopic examination, tissue preparation with routine and UEA-1 stainings was performed. Volumetric and numerical analyses of vasculature in 6 divided portions of subepithelial connective tissue were measured. The results showed that significantly greater numbers of UEA-1 staining vessels were found within the apico-superficial portion of bleeding Gingiva than those of non...
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Histometric analysis of epithelial alterations in bleeding and non-bleeding Gingivae after probing and their corresponding stereomicroscopic findings of vasculature.
National Medical Journal of China, 1991Co-Authors: Shin Nieh, Earl Fu, E-chin ShenAbstract:: The present study was carried out to investigate and compare the thickness and number of rete pegs of the sulcular epithelium histometrically in bleeding and non-bleeding Gingivae with special reference to comparisons of their relationship with corresponding stereomicroscopic findings. A total of 78 biopsies (40 bleeding and 38 non-bleeding Gingival specimens) was obtained from 33 patients. Tissue sections with H&E stains were performed. Each Gingival section was divided into three portions (coronal, middle and apical) along the Gingival sulcular epithelium horizontally. The rete pegs were counted and the thickest and thinnest parts of the epithelium in three individual portions were also measured histometrically. The results show that the epithelial thickness of bleeding Gingiva at the coronal and middle portions was significantly thinner than that of non-bleeding Gingiva. There were also greater numbers of rete pegs of the sulcular epithelium in bleeding Gingiva than of those in non-bleeding Gingiva. However, there was no specific relationship between epithelial alterations of Gingiva and the visibility of vasculature by stereomicroscopy. It is suggested that epithelial alterations of Gingiva to some extent might be responsible for the Gingival bleeding on probing clinically and highly associated with the volumetric density of underlying infiltrated connective tissue, but might not be related to the visibility of vasculature by stereomicroscopy.
Jan Cosyn - One of the best experts on this subject based on the ideXlab platform.
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the Gingival biotype revisited transparency of the periodontal probe through the Gingival margin as a method to discriminate thin from thick Gingiva
Journal of Clinical Periodontology, 2009Co-Authors: Tim De Rouck, Rouhollah Eghbali, Kristiaan Collys, Hugo De Bruyn, Jan CosynAbstract:Aim: To detect groups of subjects in a sample of 100 periodontally healthy volunteers with different combinations of morphometric data related to central maxillary incisors and surrounding soft tissues. Material and Methods: Four clinical parameters were included in a cluster analysis: crown width/crown length ratio (CW/CL), Gingival width (GW), papilla height (PH) and Gingival thickness (GT). The latter was based on the transparency of the periodontal probe through the Gingival margin while probing the buccal sulcus. Every first volunteer out of 10 was re-examined to evaluate intra-examiner repeatability for all variables. Results: High agreement between duplicate recordings was found for all parameters, in particular for GT, pointing to 85% (κ=0.70; p=0.002). The partitioning method identified three clusters with specific features. Cluster A1 (nine males, 28 females) displayed a slender tooth form (CW/CL=0.79), a GW of 4.92 mm, a PH of 4.29 mm and a thin Gingiva (probe visible on one or both incisors in 100% of the subjects). Cluster A2 (29 males, five females) presented similar features (CW/CL=0.77; GW=5.2 mm; PH=4.54 mm), except for GT. These subjects showed a clear thick Gingiva (probe concealed on both incisors in 97% of the subjects). The third group (cluster B: 12 males, 17 females) differed substantially from the other clusters in many parameters. These subjects showed a more quadratic tooth form (CW/CL=0.88), a broad zone of keratinized tissue (GW=5.84 mm), low papillae (PH=2.84 mm) and a thick Gingiva (probe concealed on both incisors in 83% of the subjects). Conclusions: The present analysis, using a simple and reproducible method for GT assessment, confirmed the existence of Gingival biotypes. A clear thin Gingiva was found in about one-third of the sample in mainly female subjects with slender teeth, a narrow zone of keratinized tissue and a highly scalloped Gingival margin corresponding to the features of the previously introduced “thin-scalloped biotype” (cluster A1). A clear thick Gingiva was found in about two-thirds of the sample in mainly male subjects. About half of them showed quadratic teeth, a broad zone of keratinized tissue and a flat Gingival margin corresponding to the features of the previously introduced “thick-flat biotype” (cluster B). The other half could not be classified as such. These subjects showed a clear thick Gingiva with slender teeth, a narrow zone of keratinized tissue and a high Gingival scallop (cluster A2).
Earl Fu - One of the best experts on this subject based on the ideXlab platform.
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histometric analysis of cell populations in Gingiva with bleeding on probing by immunohistochemistry
National Medical Journal of China, 1993Co-Authors: Earl Fu, Shih NiehAbstract:: The present study was performed by immunohistochemical methods in order to morphometrically characterize and compare the composition of cellular infiltrates in human Gingivae with bleeding and non-bleeding upon probing. A total of 64 Gingival biopsies (33 bleeding and 31 non-bleeding Gingivae) were obtained from 23 patients. Routine Hematoxylin & Eosin and immunohistochemical stains were performed following tissue preparations. On each section, three zones were histologically divided. For each zone, the percentage and histometric analysis of plasma cells, phagocytic cells, B-cells and T-cells was calculated to characterize the composition of cellular infiltrates in Gingiva associated with bleeding on probing. Significant difference was found in the middle and apical zones of infiltrated connective tissue when compared with plasma cells expressed by kappa- or lambda-chain positive immunostaining between bleeding and non-bleeding Gingivae. Significant difference was also found in the coronal zone when compared with phagocytic cells and B lymphocytes between bleeding and non-bleeding Gingivae. It is suggested that clinical bleeding tendency of Gingiva may be highly associated with histological alterations of underlying inflammatory cell populations and a complicated immunological mechanism appears to be involved.
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A stereomicroscopic and immunohistochemical study of vasculature in Gingiva bleeding after probing.
Journal of Periodontology, 1992Co-Authors: Earl Fu, Shin NiehAbstract:This study investigated the stereomicroscopic appearance of Gingival sulcular surfaces and the histologic alterations of subepithelial vasculature of Gingiva bleeding after probing. Sixty-four pairs of Gingival biopsies from 49 patients were obtained and the results of stereomicroscopically visible vasculature were observed in half of the specimens. The number of biopsies showing that the vasculature of bleeding Gingiva was significantly greater than those of non-bleeding Gingiva. The immunohistochemical marker UEA-1 was used to identify endothelial cells on the vascular walls of 34 Gingival biopsies from 15 patients. Following stereomicroscopic examination, tissue preparation with routine and UEA-1 stainings was performed. Volumetric and numerical analyses of vasculature in 6 divided portions of subepithelial connective tissue were measured. The results showed that significantly greater numbers of UEA-1 staining vessels were found within the apico-superficial portion of bleeding Gingiva than those of non...
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Histometric analysis of epithelial alterations in bleeding and non-bleeding Gingivae after probing and their corresponding stereomicroscopic findings of vasculature.
National Medical Journal of China, 1991Co-Authors: Shin Nieh, Earl Fu, E-chin ShenAbstract:: The present study was carried out to investigate and compare the thickness and number of rete pegs of the sulcular epithelium histometrically in bleeding and non-bleeding Gingivae with special reference to comparisons of their relationship with corresponding stereomicroscopic findings. A total of 78 biopsies (40 bleeding and 38 non-bleeding Gingival specimens) was obtained from 33 patients. Tissue sections with H&E stains were performed. Each Gingival section was divided into three portions (coronal, middle and apical) along the Gingival sulcular epithelium horizontally. The rete pegs were counted and the thickest and thinnest parts of the epithelium in three individual portions were also measured histometrically. The results show that the epithelial thickness of bleeding Gingiva at the coronal and middle portions was significantly thinner than that of non-bleeding Gingiva. There were also greater numbers of rete pegs of the sulcular epithelium in bleeding Gingiva than of those in non-bleeding Gingiva. However, there was no specific relationship between epithelial alterations of Gingiva and the visibility of vasculature by stereomicroscopy. It is suggested that epithelial alterations of Gingiva to some extent might be responsible for the Gingival bleeding on probing clinically and highly associated with the volumetric density of underlying infiltrated connective tissue, but might not be related to the visibility of vasculature by stereomicroscopy.