Stereomicroscopy

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Andreas Taeger - One of the best experts on this subject based on the ideXlab platform.

  • Pachyprotasis kojimai Taeger & Shinohara, 2017, paratype female (NSMT)
    2017
    Co-Authors: Andreas Taeger
    Abstract:

    Additional figures for the paper:Pachyprotasis kojimai sp. nov. — the “Pachyprotasis nigronotata” of Japanese authors (Hymenoptera: Tenthredinidae) by Andreas Taeger, Akihiko Shinohara & Katja Kramp (Zootaxa, 2017)Photos were taken by the first author with a Leica DFC 495 digital camera and a Leica M405 C stereomicroscope. Composite images with an extended depth of field were created from stacks of images using the software CombineZP, and finally arranged and partly enhanced with Ulead PhotoImpact X3.

  • Pachyprotasis kojimai Taeger & Shinohara, 2017, male (NSMT)
    2017
    Co-Authors: Andreas Taeger
    Abstract:

    Additional figures for the paper:Pachyprotasis kojimai sp. nov.—the “Pachyprotasis nigronotata” of Japanese authors (Hymenoptera: Tenthredinidae) by Andreas Taeger, Akihiko Shinohara & Katja Kramp (Zootaxa, 2017)Photos were taken by the first author with a Leica DFC 495 digital camera and a Leica M405 C stereomicroscope. Composite images with an extended depth of field were created from stacks of images using the software CombineZP, and finally arranged and partly enhanced with Ulead PhotoImpact X3.

  • Pachyprotasis kojimai Taeger & Shinohara, 2017, holotype (NSMT)
    2017
    Co-Authors: Andreas Taeger
    Abstract:

    Additional figures for the paper:Pachyprotasis kojimai sp. nov. — the “Pachyprotasis nigronotata” of Japanese authors (Hymenoptera: Tenthredinidae) by Andreas Taeger, Akihiko Shinohara & Katja Kramp (Zootaxa, 2017)Photos were taken by the first author with a Leica DFC 495 digital camera and a Leica M405 C stereomicroscope. Composite images with an extended depth of field were created from stacks of images using the software CombineZP, and finally arranged and partly enhanced with Ulead PhotoImpact X3.

  • Pachyprotasis simulans (Klug, 1817) from Germany
    2017
    Co-Authors: Andreas Taeger
    Abstract:

    Additional figures for the paper:Pachyprotasis kojimai sp. nov.—the “Pachyprotasis nigronotata” of Japanese authors (Hymenoptera: Tenthredinidae) by Andreas Taeger, Akihiko Shinohara & Katja Kramp (Zootaxa, 2017)Photos were taken by the first author with a Leica DFC 495 digital camera and a Leica M405 C stereomicroscope. Composite images with an extended depth of field were created from stacks of images using the software CombineZP, and finally arranged and partly enhanced with Ulead PhotoImpact X3.

  • Pachyprotasis kojimai Taeger & Shinohara, 2017, male (SDEI)
    2017
    Co-Authors: Andreas Taeger
    Abstract:

    Additional figures for the paper:Pachyprotasis kojimai sp. nov. — the “Pachyprotasis nigronotata” of Japanese authors (Hymenoptera: Tenthredinidae) by Andreas Taeger, Akihiko Shinohara & Katja Kramp (Zootaxa, 2017)Photos were taken by the first author with a Leica DFC 495 digital camera and a Leica M405 C stereomicroscope. Composite images with an extended depth of field were created from stacks of images using the software CombineZP, and finally arranged and partly enhanced with Ulead PhotoImpact X3.

Andrea Seitun - One of the best experts on this subject based on the ideXlab platform.

A Cupello - One of the best experts on this subject based on the ideXlab platform.

Barth F Smets - One of the best experts on this subject based on the ideXlab platform.

  • novel assay to assess permissiveness of a soil microbial community toward receipt of mobile genetic elements
    Applied and Environmental Microbiology, 2010
    Co-Authors: Sanin Musovic, Arnaud Dechesne, Jan Tind Sorensen, Barth F Smets
    Abstract:

    There is a wealth of evidence indicating that mobile genetic elements can spread in natural microbial communities. However, little is known regarding the fraction of the community that actually engages in this behavior. Here we report on a new approach to quantify the fraction of a bacterial community that is able to receive and maintain an exogenous conjugal plasmid termed community permissiveness. Conjugal transfer of a broad-host-range plasmid labeled with a zygotically inducible green fluorescent protein (RP4::gfp) from a donor strain (Pseudomonas putida) to a soil bacterial suspension was examined. The mixture of cells was incubated on membrane filters supported by different solid media. Plasmid transfer was scored by in situ visualization of green fluorescent transconjugant microcolonies, and host range was determined by traditional plating or microcolony isolation by using a micromanipulator. Among the conditions tested, the highest plasmid transfer incidence (approximately 1 transfer per 10(4) soil bacteria) was measured after 48 h of incubation on either a 10% soil extract or a 10-fold diluted R2A medium. Stereomicroscopy combined with image analysis allowed easy examination and enumeration of green fluorescent microcolonies. In all experiments, however, Stereomicroscopy consistently underestimated the number of conjugation events (approximately 10-fold) in comparison to confocal laser scanning microscopy. The plasmid host range was broad and included bacteria belonging to the Alphaproteobacteria, Betaproteobacteria, and Gammaproteobacteria classes of proteobacteria. The isolation of transconjugant microcolonies by micromanipulation greatly extended the estimated plasmid host range among soil bacteria. The new approach can be applied to examine the permissiveness of various communities toward receipt of different mobile elements.

M V Rapallino - One of the best experts on this subject based on the ideXlab platform.