The Experts below are selected from a list of 207 Experts worldwide ranked by ideXlab platform
Jun Chen - One of the best experts on this subject based on the ideXlab platform.
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pressure and electrokinetic injections for on line sample stacking neutral analytes in microemulsion electrokinetic chromatography with salt containing matrixes
Electrophoresis, 2008Co-Authors: Jun Cao, Jun ChenAbstract:An on-line technique for pressure and electrokinetic injections of long sample plugs with simultaneous stacking of neutral analytes (notoGinsenoside R 1 , Ginsenoside Rg 1 , Ginsenoside Rf, Ginsenoside Rh 1 , Ginsenoside Rd, Ginsenoside Rg 3 ) in microemulsion electrokinetic chromatography is presented. The effects of salt concentration, sample plug length, oRganic modification of the sample matrix, oil phase and SDS concentration on stacking efficiency were examined in order to optimize the two injection methods. In microemulsion electrokinetic chromatography, the effect of the type of oil and SDS content on stacking mechanism is often sophisticated. This study had demonstrated that the oil type and SDS content in microemulsion indeed markedly altered the affinity of microemulsion with analytes. Finally, in comparison with the electrokinetic injection method, the most apparent disadvantages of the pressure injection method were the relatively high LOD and poor reproducibility.
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Pressure and electrokinetic injections for on‐line sample stacking neutral analytes in microemulsion electrokinetic chromatography with salt‐containing matrixes
Electrophoresis, 2008Co-Authors: Jun Cao, Jun ChenAbstract:An on-line technique for pressure and electrokinetic injections of long sample plugs with simultaneous stacking of neutral analytes (notoGinsenoside R 1 , Ginsenoside Rg 1 , Ginsenoside Rf, Ginsenoside Rh 1 , Ginsenoside Rd, Ginsenoside Rg 3 ) in microemulsion electrokinetic chromatography is presented. The effects of salt concentration, sample plug length, oRganic modification of the sample matrix, oil phase and SDS concentration on stacking efficiency were examined in order to optimize the two injection methods. In microemulsion electrokinetic chromatography, the effect of the type of oil and SDS content on stacking mechanism is often sophisticated. This study had demonstrated that the oil type and SDS content in microemulsion indeed markedly altered the affinity of microemulsion with analytes. Finally, in comparison with the electrokinetic injection method, the most apparent disadvantages of the pressure injection method were the relatively high LOD and poor reproducibility.
Jun Cao - One of the best experts on this subject based on the ideXlab platform.
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pressure and electrokinetic injections for on line sample stacking neutral analytes in microemulsion electrokinetic chromatography with salt containing matrixes
Electrophoresis, 2008Co-Authors: Jun Cao, Jun ChenAbstract:An on-line technique for pressure and electrokinetic injections of long sample plugs with simultaneous stacking of neutral analytes (notoGinsenoside R 1 , Ginsenoside Rg 1 , Ginsenoside Rf, Ginsenoside Rh 1 , Ginsenoside Rd, Ginsenoside Rg 3 ) in microemulsion electrokinetic chromatography is presented. The effects of salt concentration, sample plug length, oRganic modification of the sample matrix, oil phase and SDS concentration on stacking efficiency were examined in order to optimize the two injection methods. In microemulsion electrokinetic chromatography, the effect of the type of oil and SDS content on stacking mechanism is often sophisticated. This study had demonstrated that the oil type and SDS content in microemulsion indeed markedly altered the affinity of microemulsion with analytes. Finally, in comparison with the electrokinetic injection method, the most apparent disadvantages of the pressure injection method were the relatively high LOD and poor reproducibility.
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Pressure and electrokinetic injections for on‐line sample stacking neutral analytes in microemulsion electrokinetic chromatography with salt‐containing matrixes
Electrophoresis, 2008Co-Authors: Jun Cao, Jun ChenAbstract:An on-line technique for pressure and electrokinetic injections of long sample plugs with simultaneous stacking of neutral analytes (notoGinsenoside R 1 , Ginsenoside Rg 1 , Ginsenoside Rf, Ginsenoside Rh 1 , Ginsenoside Rd, Ginsenoside Rg 3 ) in microemulsion electrokinetic chromatography is presented. The effects of salt concentration, sample plug length, oRganic modification of the sample matrix, oil phase and SDS concentration on stacking efficiency were examined in order to optimize the two injection methods. In microemulsion electrokinetic chromatography, the effect of the type of oil and SDS content on stacking mechanism is often sophisticated. This study had demonstrated that the oil type and SDS content in microemulsion indeed markedly altered the affinity of microemulsion with analytes. Finally, in comparison with the electrokinetic injection method, the most apparent disadvantages of the pressure injection method were the relatively high LOD and poor reproducibility.
Ruo-yi Liao - One of the best experts on this subject based on the ideXlab platform.
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Ginsenoside Rg 1 promoted the wound closure of diabetic foot ulcer through inos elevation via mir 23a irf 1 axis
Life Sciences, 2019Co-Authors: Hua-an Cai, Liang Huang, Li-jun Zheng, Jing Wang, Ruo-yi LiaoAbstract:Abstract Background Impaired wound healing in diabetes foot ulcers (DFUs) brings a great burden to diabetic patients. Pro-angiogenesis through elevating nitric oxide (NO) is beneficial to the wound healing process. Ginsenoside Rg1, the main active in Notoginseng, is reported to regulate the angiogenesis in endothelial cells through modulating miR-23a. However, the effect of Rg1 in diabetes remains elusive. Methods High fat diet combined with streptozotocin-induced diabetic rats were treated with Rg1. Then incision area and tissue NO level were measured to evaluate the wound closure efficacy of Rg1. Then high glucose cultured HUVECs were employed to mimic diabetic environment in vitro. Overexpression and knockdown plasmids of miR-23a or IRF-1 were constructed and transfected in HUVECs. qPCR and western blot were used to determine the mRNA and protein level, respectively. Dual-luciferase reporter assay was utilized to determine the interaction of IRF-1/miR-23a. Results Rg1 accelerated the wound closure speed in diabetic rats and increased NO level through elevating iNOS expression. Knockdown of iNOS reversed Rg1-induced VEGF expression, cell proliferation, anti-apoptotic efficacy and cell migration ability in high glucose cultured HUVECs. Further investigation revealed that Rg1 mediated iNOS through miR-23a. miR-23a inhibited the expression of IRF-1, a protein which could directly bind to the iNOS mRNA 3’UTR. Conclusion Rg1 promoted angiogenesis in diabetic wound healing process through NO signaling via miR-23a, providing a novel candidate for DFUs treatment.
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Ginsenoside (Rg-1) promoted the wound closure of diabetic foot ulcer through iNOS elevation via miR-23a/IRF-1 axis.
Life sciences, 2019Co-Authors: Hua-an Cai, Liang Huang, Li-jun Zheng, Jing Wang, Ruo-yi LiaoAbstract:Abstract Background Impaired wound healing in diabetes foot ulcers (DFUs) brings a great burden to diabetic patients. Pro-angiogenesis through elevating nitric oxide (NO) is beneficial to the wound healing process. Ginsenoside Rg1, the main active in Notoginseng, is reported to regulate the angiogenesis in endothelial cells through modulating miR-23a. However, the effect of Rg1 in diabetes remains elusive. Methods High fat diet combined with streptozotocin-induced diabetic rats were treated with Rg1. Then incision area and tissue NO level were measured to evaluate the wound closure efficacy of Rg1. Then high glucose cultured HUVECs were employed to mimic diabetic environment in vitro. Overexpression and knockdown plasmids of miR-23a or IRF-1 were constructed and transfected in HUVECs. qPCR and western blot were used to determine the mRNA and protein level, respectively. Dual-luciferase reporter assay was utilized to determine the interaction of IRF-1/miR-23a. Results Rg1 accelerated the wound closure speed in diabetic rats and increased NO level through elevating iNOS expression. Knockdown of iNOS reversed Rg1-induced VEGF expression, cell proliferation, anti-apoptotic efficacy and cell migration ability in high glucose cultured HUVECs. Further investigation revealed that Rg1 mediated iNOS through miR-23a. miR-23a inhibited the expression of IRF-1, a protein which could directly bind to the iNOS mRNA 3’UTR. Conclusion Rg1 promoted angiogenesis in diabetic wound healing process through NO signaling via miR-23a, providing a novel candidate for DFUs treatment.
Hua-an Cai - One of the best experts on this subject based on the ideXlab platform.
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Ginsenoside Rg 1 promoted the wound closure of diabetic foot ulcer through inos elevation via mir 23a irf 1 axis
Life Sciences, 2019Co-Authors: Hua-an Cai, Liang Huang, Li-jun Zheng, Jing Wang, Ruo-yi LiaoAbstract:Abstract Background Impaired wound healing in diabetes foot ulcers (DFUs) brings a great burden to diabetic patients. Pro-angiogenesis through elevating nitric oxide (NO) is beneficial to the wound healing process. Ginsenoside Rg1, the main active in Notoginseng, is reported to regulate the angiogenesis in endothelial cells through modulating miR-23a. However, the effect of Rg1 in diabetes remains elusive. Methods High fat diet combined with streptozotocin-induced diabetic rats were treated with Rg1. Then incision area and tissue NO level were measured to evaluate the wound closure efficacy of Rg1. Then high glucose cultured HUVECs were employed to mimic diabetic environment in vitro. Overexpression and knockdown plasmids of miR-23a or IRF-1 were constructed and transfected in HUVECs. qPCR and western blot were used to determine the mRNA and protein level, respectively. Dual-luciferase reporter assay was utilized to determine the interaction of IRF-1/miR-23a. Results Rg1 accelerated the wound closure speed in diabetic rats and increased NO level through elevating iNOS expression. Knockdown of iNOS reversed Rg1-induced VEGF expression, cell proliferation, anti-apoptotic efficacy and cell migration ability in high glucose cultured HUVECs. Further investigation revealed that Rg1 mediated iNOS through miR-23a. miR-23a inhibited the expression of IRF-1, a protein which could directly bind to the iNOS mRNA 3’UTR. Conclusion Rg1 promoted angiogenesis in diabetic wound healing process through NO signaling via miR-23a, providing a novel candidate for DFUs treatment.
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Ginsenoside (Rg-1) promoted the wound closure of diabetic foot ulcer through iNOS elevation via miR-23a/IRF-1 axis.
Life sciences, 2019Co-Authors: Hua-an Cai, Liang Huang, Li-jun Zheng, Jing Wang, Ruo-yi LiaoAbstract:Abstract Background Impaired wound healing in diabetes foot ulcers (DFUs) brings a great burden to diabetic patients. Pro-angiogenesis through elevating nitric oxide (NO) is beneficial to the wound healing process. Ginsenoside Rg1, the main active in Notoginseng, is reported to regulate the angiogenesis in endothelial cells through modulating miR-23a. However, the effect of Rg1 in diabetes remains elusive. Methods High fat diet combined with streptozotocin-induced diabetic rats were treated with Rg1. Then incision area and tissue NO level were measured to evaluate the wound closure efficacy of Rg1. Then high glucose cultured HUVECs were employed to mimic diabetic environment in vitro. Overexpression and knockdown plasmids of miR-23a or IRF-1 were constructed and transfected in HUVECs. qPCR and western blot were used to determine the mRNA and protein level, respectively. Dual-luciferase reporter assay was utilized to determine the interaction of IRF-1/miR-23a. Results Rg1 accelerated the wound closure speed in diabetic rats and increased NO level through elevating iNOS expression. Knockdown of iNOS reversed Rg1-induced VEGF expression, cell proliferation, anti-apoptotic efficacy and cell migration ability in high glucose cultured HUVECs. Further investigation revealed that Rg1 mediated iNOS through miR-23a. miR-23a inhibited the expression of IRF-1, a protein which could directly bind to the iNOS mRNA 3’UTR. Conclusion Rg1 promoted angiogenesis in diabetic wound healing process through NO signaling via miR-23a, providing a novel candidate for DFUs treatment.
Xing Zeng - One of the best experts on this subject based on the ideXlab platform.
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Determination of Ginsenoside-Rg1 in human plasma and its application to pharmacokinetic studies following intravenous administration of ‘Shenmai’ injection
Phytotherapy research : PTR, 2009Co-Authors: Liu Yang, Yiming Liu, Xing ZengAbstract:'Shenmai' injection is derived from traditional Chinese medicine 'Shenmaisan' and made from Radix ginseng Rubra and Radix Ophiopogonis. Ginsenoside-Rg(1), as a major constituent of Radix ginseng Rubra, is considered responsible for the efficacy of this injection. A rapid, simple and accurate method has been established for determination of Ginsenoside-Rg(1) in Shenmai injection and human plasma using LC-ESI-MS/MS, and to study the pharmacokinetics of Rg(1) in ten healthy volunteers after intravenous single dosing of 60 mL of Shenmai injection. Following solid-phase extraction (SPE), samples were separated on a C(18) column coupled with electrospray ionization mass spectrometry. The protonated analyte was quantified by multiple reaction monitoring (MRM) with a quadruple mass spectrometer in positive mode. Linearity was confirmed in the concentration range of 1 to 1000 ng/mL for Rg(1), and the lower limit of quantification (LLOQ, S/N > 10) was 1 ng/mL. The intraday and interday RSDs were within 15% and mean extraction recoveries ranged from 98.6% to 104.9%. The pharmacokinetics of Rg(1) in healthy volunteers conforms to the two-compartment open model. The main pharmacokinetics parameters were as follows: t(1/2beta), 2.09 +/- 1.89 h; CL, 0.03 +/- 0.01 L kg(-1) h(-1); AUC (0 approximately infinity), 124.4 +/- 35.9 2 ng mL(-1) h and AUC (0 approximately infinity), 127.9 +/- 37.2 ng mL(-1) h, respectively.