The Experts below are selected from a list of 225 Experts worldwide ranked by ideXlab platform

M E Figueroa - One of the best experts on this subject based on the ideXlab platform.

  • effects of soil physicochemical properties on plant performance of Glaucium flavum crantz
    Plant and Soil, 2015
    Co-Authors: J Cambrolle, Sara Munozvalles, J M Mancillaleyton, L Andradesmoreno, T Luque, M E Figueroa
    Abstract:

    Background and aims Glaucium flavum is an endangered coastal herb capable of growth in a wide variety of ecosystems. We explore plant-soil relationships in this species by evaluating plant performance and soil physicochemical properties in the different types of ecosystems where it grows.

  • Effects of soil physicochemical properties on plant performance of Glaucium flavum Crantz
    Plant and Soil, 2015
    Co-Authors: J Cambrolle, T Luque, S. Muñoz-vallés, J. M. Mancilla-leytón, L. Andrades-moreno, M E Figueroa
    Abstract:

    Background and aims Glaucium flavum is an endangered coastal herb capable of growth in a wide variety of ecosystems. We explore plant-soil relationships in this species by evaluating plant performance and soil physicochemical properties in the different types of ecosystems where it grows. Methods Ten natural populations from southern Spain were chosen for the study. In each experimental site, a number of variables related to the growth, reproduction and nutritional status of G. flavum were studied and an analysis of the soil physicochemical properties (texture, pH, electrical conductivity and total N, P, S and Ca concentration) was conducted. Data were statistically analyzed in order to characterize the habitat of G. flavum and to explore possible relationships between the studied variables. Results At all experimental sites, the soil presented an alkaline pH and high concentrations of Ca. Despite the low levels of soil N, P and S, our data did not indicate nutrient deficiencies in leaf tissues. The percentage of reproductive plants and number of seeds per fruit correlated positively with leaf Ca concentrations. Conclusions Increasing Ca concentrations in plant tissues and elevated soil pH have a positive effect on plant performance of G. flavum .

  • Seasonal ecophysiology of an endangered coastal species, the yellow-horned poppy (Glaucium flavum Crantz)
    Russian Journal of Ecology, 2014
    Co-Authors: J Cambrolle, S. Redondo-gómez, E. Mateos-naranjo, T Luque, M E Figueroa
    Abstract:

    Glaucium flavum Crantz. is a short-lived perennial herb distributed in coastal zones from the Black Sea to southern, western and north-western Europe. Despite its diminishing area of distribution and potential pharmacological value, little is known about the ecophysiological features of this coastal species. We investigated the photosynthetic performance of G. flavum by measuring gas exchange, chlorophyll fluorescence, photosynthetic pigment concentration and leaf water content over the space of a year in a coastal habitat of SW Spain. We also measured the variation in total concentrations of nitrogen, phosphorus, sulphur, potassium, calcium and magnesium, in the leaves and soil, throughout the study period. G. flavum showed a high resistance to summer drought conditions which appeared to be due to the high degree of stomatal control. The potential photochemical efficiency of photosystem II showed minimum values during the winter, indicating that low temperatures can produce negative effects within the photosynthetic apparatus. However, the marked decline in net photosynthesis during the winter seems to be mainly related to a loss of metabolic activity. Although leaf nutrient concentrations were, in general, within the normal ranges, phosphorus availability seems to be limited by the high calcium concentrations detected in the soil of the study site. Our study points out the efficiency of the different physiological adaptations of this rare and endangered coastal species in coping with the strong seasonal variability of the Mediterranean climate.

  • growth reproductive and photosynthetic responses to copper in the yellow horned poppy Glaucium flavum crantz
    Environmental and Experimental Botany, 2011
    Co-Authors: J Cambrolle, T Luque, Susana Redondogomez, Enrique Mateosnaranjo, M E Figueroa
    Abstract:

    Abstract Glaucium flavum Crantz. is found in an anthropized coastal grassland at the joint estuary of the Tinto and Odiel rivers (SW Spain), growing under the influence of high levels of copper contamination derived from nearby petrochemical industries, with no obvious adverse affects on the performance of the plant. In addition, this species exhibits a series of ecological characteristics which may render it appropriate for use in the phytoremediation of contaminated areas. Nonetheless, the response of G. flavum to elevated copper concentrations has not been studied. A greenhouse experiment was conducted to investigate the effects of a range of Cu concentrations (0 to 47 mmol l −1 ) on the growth, reproduction and photosynthetic performance of G. flavum , by measuring relative growth rate, fruit and seed production, chlorophyll fluorescence parameters, gas exchange and photosynthetic pigment concentrations. We also determined total copper, nitrogen, phosphorous, sulphur, calcium and magnesium concentrations. G. flavum survived with concentrations of up to 730 mg Cu kg −1 DW in the leaves, when treated with 30 mmol Cu l −1 (2000 mg l −1 ). Quantum efficiency of PSII, net photosynthesis rate, as well as leaf Ca and Mg concentrations were all negatively affected by Cu concentrations greater than 9 mmol l −1 in the nutrient solution. Our results indicate that the reduction in photosynthetic performance may be attributed to the adverse effect of excess Cu on the photosynthetic apparatus of the plant, both directly, via a decrease in pigment concentrations, and indirectly, via interference of Cu with Ca ions of PSII. Growth and seed production were only slightly affected by leaf tissue concentrations as high as 230 mg Cu kg −1 dry mass, which suggests that this species could play an important role in phytoremediation of Cu-contaminated soils.

  • physiological responses to salinity in the yellow horned poppy Glaucium flavum
    Plant Physiology and Biochemistry, 2011
    Co-Authors: J Cambrolle, T Luque, Susana Redondogomez, Enrique Mateosnaranjo, M E Figueroa
    Abstract:

    Abstract Glaucium flavum Crantz. is a short-lived perennial herb found in coastal habitats in southern Spain growing under a wide range of interstitial soil salinity levels, from that of fresh water up to the high concentration typical of sea water. An experiment was designed to investigate the effect of exposure to this range of salinity on the photosynthetic apparatus, growth and reproduction of G. flavum , by measuring relative growth rate, percentage of dead leaves, seed production, leaf relative water content, chlorophyll fluorescence parameters, gas exchange and photosynthetic pigment concentrations. We also determined total sodium, potassium, calcium, magnesium, and nitrogen concentrations. G. flavum survived at NaCl concentrations as high as 300 mM, although the excess of NaCl resulted in a biomass reduction of between 26 and 76% (in 60 and 300 mM NaCl treatments, respectively). The long-term effects of salinity on the growth and reproduction of G. flavum were mainly linked to an overall reduction in carbon gain as a result of stomatal conductance regulation. Also, the excess of salt caused a reduction in pigment concentrations, as well as Ca-, Mg- and N-uptake. The results indicate that, in the presence of excess soil-water salinity, G. flavum sustains little overall effects on the photochemical (PSII) apparatus, and is capable of tolerating a very high and continued exposure to salinity by maintaining low levels of net photosynthesis.

J Cambrolle - One of the best experts on this subject based on the ideXlab platform.

  • effects of soil physicochemical properties on plant performance of Glaucium flavum crantz
    Plant and Soil, 2015
    Co-Authors: J Cambrolle, Sara Munozvalles, J M Mancillaleyton, L Andradesmoreno, T Luque, M E Figueroa
    Abstract:

    Background and aims Glaucium flavum is an endangered coastal herb capable of growth in a wide variety of ecosystems. We explore plant-soil relationships in this species by evaluating plant performance and soil physicochemical properties in the different types of ecosystems where it grows.

  • Effects of soil physicochemical properties on plant performance of Glaucium flavum Crantz
    Plant and Soil, 2015
    Co-Authors: J Cambrolle, T Luque, S. Muñoz-vallés, J. M. Mancilla-leytón, L. Andrades-moreno, M E Figueroa
    Abstract:

    Background and aims Glaucium flavum is an endangered coastal herb capable of growth in a wide variety of ecosystems. We explore plant-soil relationships in this species by evaluating plant performance and soil physicochemical properties in the different types of ecosystems where it grows. Methods Ten natural populations from southern Spain were chosen for the study. In each experimental site, a number of variables related to the growth, reproduction and nutritional status of G. flavum were studied and an analysis of the soil physicochemical properties (texture, pH, electrical conductivity and total N, P, S and Ca concentration) was conducted. Data were statistically analyzed in order to characterize the habitat of G. flavum and to explore possible relationships between the studied variables. Results At all experimental sites, the soil presented an alkaline pH and high concentrations of Ca. Despite the low levels of soil N, P and S, our data did not indicate nutrient deficiencies in leaf tissues. The percentage of reproductive plants and number of seeds per fruit correlated positively with leaf Ca concentrations. Conclusions Increasing Ca concentrations in plant tissues and elevated soil pH have a positive effect on plant performance of G. flavum .

  • Seasonal ecophysiology of an endangered coastal species, the yellow-horned poppy (Glaucium flavum Crantz)
    Russian Journal of Ecology, 2014
    Co-Authors: J Cambrolle, S. Redondo-gómez, E. Mateos-naranjo, T Luque, M E Figueroa
    Abstract:

    Glaucium flavum Crantz. is a short-lived perennial herb distributed in coastal zones from the Black Sea to southern, western and north-western Europe. Despite its diminishing area of distribution and potential pharmacological value, little is known about the ecophysiological features of this coastal species. We investigated the photosynthetic performance of G. flavum by measuring gas exchange, chlorophyll fluorescence, photosynthetic pigment concentration and leaf water content over the space of a year in a coastal habitat of SW Spain. We also measured the variation in total concentrations of nitrogen, phosphorus, sulphur, potassium, calcium and magnesium, in the leaves and soil, throughout the study period. G. flavum showed a high resistance to summer drought conditions which appeared to be due to the high degree of stomatal control. The potential photochemical efficiency of photosystem II showed minimum values during the winter, indicating that low temperatures can produce negative effects within the photosynthetic apparatus. However, the marked decline in net photosynthesis during the winter seems to be mainly related to a loss of metabolic activity. Although leaf nutrient concentrations were, in general, within the normal ranges, phosphorus availability seems to be limited by the high calcium concentrations detected in the soil of the study site. Our study points out the efficiency of the different physiological adaptations of this rare and endangered coastal species in coping with the strong seasonal variability of the Mediterranean climate.

  • growth reproductive and photosynthetic responses to copper in the yellow horned poppy Glaucium flavum crantz
    Environmental and Experimental Botany, 2011
    Co-Authors: J Cambrolle, T Luque, Susana Redondogomez, Enrique Mateosnaranjo, M E Figueroa
    Abstract:

    Abstract Glaucium flavum Crantz. is found in an anthropized coastal grassland at the joint estuary of the Tinto and Odiel rivers (SW Spain), growing under the influence of high levels of copper contamination derived from nearby petrochemical industries, with no obvious adverse affects on the performance of the plant. In addition, this species exhibits a series of ecological characteristics which may render it appropriate for use in the phytoremediation of contaminated areas. Nonetheless, the response of G. flavum to elevated copper concentrations has not been studied. A greenhouse experiment was conducted to investigate the effects of a range of Cu concentrations (0 to 47 mmol l −1 ) on the growth, reproduction and photosynthetic performance of G. flavum , by measuring relative growth rate, fruit and seed production, chlorophyll fluorescence parameters, gas exchange and photosynthetic pigment concentrations. We also determined total copper, nitrogen, phosphorous, sulphur, calcium and magnesium concentrations. G. flavum survived with concentrations of up to 730 mg Cu kg −1 DW in the leaves, when treated with 30 mmol Cu l −1 (2000 mg l −1 ). Quantum efficiency of PSII, net photosynthesis rate, as well as leaf Ca and Mg concentrations were all negatively affected by Cu concentrations greater than 9 mmol l −1 in the nutrient solution. Our results indicate that the reduction in photosynthetic performance may be attributed to the adverse effect of excess Cu on the photosynthetic apparatus of the plant, both directly, via a decrease in pigment concentrations, and indirectly, via interference of Cu with Ca ions of PSII. Growth and seed production were only slightly affected by leaf tissue concentrations as high as 230 mg Cu kg −1 dry mass, which suggests that this species could play an important role in phytoremediation of Cu-contaminated soils.

  • physiological responses to salinity in the yellow horned poppy Glaucium flavum
    Plant Physiology and Biochemistry, 2011
    Co-Authors: J Cambrolle, T Luque, Susana Redondogomez, Enrique Mateosnaranjo, M E Figueroa
    Abstract:

    Abstract Glaucium flavum Crantz. is a short-lived perennial herb found in coastal habitats in southern Spain growing under a wide range of interstitial soil salinity levels, from that of fresh water up to the high concentration typical of sea water. An experiment was designed to investigate the effect of exposure to this range of salinity on the photosynthetic apparatus, growth and reproduction of G. flavum , by measuring relative growth rate, percentage of dead leaves, seed production, leaf relative water content, chlorophyll fluorescence parameters, gas exchange and photosynthetic pigment concentrations. We also determined total sodium, potassium, calcium, magnesium, and nitrogen concentrations. G. flavum survived at NaCl concentrations as high as 300 mM, although the excess of NaCl resulted in a biomass reduction of between 26 and 76% (in 60 and 300 mM NaCl treatments, respectively). The long-term effects of salinity on the growth and reproduction of G. flavum were mainly linked to an overall reduction in carbon gain as a result of stomatal conductance regulation. Also, the excess of salt caused a reduction in pigment concentrations, as well as Ca-, Mg- and N-uptake. The results indicate that, in the presence of excess soil-water salinity, G. flavum sustains little overall effects on the photochemical (PSII) apparatus, and is capable of tolerating a very high and continued exposure to salinity by maintaining low levels of net photosynthesis.

T Luque - One of the best experts on this subject based on the ideXlab platform.

  • effects of soil physicochemical properties on plant performance of Glaucium flavum crantz
    Plant and Soil, 2015
    Co-Authors: J Cambrolle, Sara Munozvalles, J M Mancillaleyton, L Andradesmoreno, T Luque, M E Figueroa
    Abstract:

    Background and aims Glaucium flavum is an endangered coastal herb capable of growth in a wide variety of ecosystems. We explore plant-soil relationships in this species by evaluating plant performance and soil physicochemical properties in the different types of ecosystems where it grows.

  • Effects of soil physicochemical properties on plant performance of Glaucium flavum Crantz
    Plant and Soil, 2015
    Co-Authors: J Cambrolle, T Luque, S. Muñoz-vallés, J. M. Mancilla-leytón, L. Andrades-moreno, M E Figueroa
    Abstract:

    Background and aims Glaucium flavum is an endangered coastal herb capable of growth in a wide variety of ecosystems. We explore plant-soil relationships in this species by evaluating plant performance and soil physicochemical properties in the different types of ecosystems where it grows. Methods Ten natural populations from southern Spain were chosen for the study. In each experimental site, a number of variables related to the growth, reproduction and nutritional status of G. flavum were studied and an analysis of the soil physicochemical properties (texture, pH, electrical conductivity and total N, P, S and Ca concentration) was conducted. Data were statistically analyzed in order to characterize the habitat of G. flavum and to explore possible relationships between the studied variables. Results At all experimental sites, the soil presented an alkaline pH and high concentrations of Ca. Despite the low levels of soil N, P and S, our data did not indicate nutrient deficiencies in leaf tissues. The percentage of reproductive plants and number of seeds per fruit correlated positively with leaf Ca concentrations. Conclusions Increasing Ca concentrations in plant tissues and elevated soil pH have a positive effect on plant performance of G. flavum .

  • Seasonal ecophysiology of an endangered coastal species, the yellow-horned poppy (Glaucium flavum Crantz)
    Russian Journal of Ecology, 2014
    Co-Authors: J Cambrolle, S. Redondo-gómez, E. Mateos-naranjo, T Luque, M E Figueroa
    Abstract:

    Glaucium flavum Crantz. is a short-lived perennial herb distributed in coastal zones from the Black Sea to southern, western and north-western Europe. Despite its diminishing area of distribution and potential pharmacological value, little is known about the ecophysiological features of this coastal species. We investigated the photosynthetic performance of G. flavum by measuring gas exchange, chlorophyll fluorescence, photosynthetic pigment concentration and leaf water content over the space of a year in a coastal habitat of SW Spain. We also measured the variation in total concentrations of nitrogen, phosphorus, sulphur, potassium, calcium and magnesium, in the leaves and soil, throughout the study period. G. flavum showed a high resistance to summer drought conditions which appeared to be due to the high degree of stomatal control. The potential photochemical efficiency of photosystem II showed minimum values during the winter, indicating that low temperatures can produce negative effects within the photosynthetic apparatus. However, the marked decline in net photosynthesis during the winter seems to be mainly related to a loss of metabolic activity. Although leaf nutrient concentrations were, in general, within the normal ranges, phosphorus availability seems to be limited by the high calcium concentrations detected in the soil of the study site. Our study points out the efficiency of the different physiological adaptations of this rare and endangered coastal species in coping with the strong seasonal variability of the Mediterranean climate.

  • growth reproductive and photosynthetic responses to copper in the yellow horned poppy Glaucium flavum crantz
    Environmental and Experimental Botany, 2011
    Co-Authors: J Cambrolle, T Luque, Susana Redondogomez, Enrique Mateosnaranjo, M E Figueroa
    Abstract:

    Abstract Glaucium flavum Crantz. is found in an anthropized coastal grassland at the joint estuary of the Tinto and Odiel rivers (SW Spain), growing under the influence of high levels of copper contamination derived from nearby petrochemical industries, with no obvious adverse affects on the performance of the plant. In addition, this species exhibits a series of ecological characteristics which may render it appropriate for use in the phytoremediation of contaminated areas. Nonetheless, the response of G. flavum to elevated copper concentrations has not been studied. A greenhouse experiment was conducted to investigate the effects of a range of Cu concentrations (0 to 47 mmol l −1 ) on the growth, reproduction and photosynthetic performance of G. flavum , by measuring relative growth rate, fruit and seed production, chlorophyll fluorescence parameters, gas exchange and photosynthetic pigment concentrations. We also determined total copper, nitrogen, phosphorous, sulphur, calcium and magnesium concentrations. G. flavum survived with concentrations of up to 730 mg Cu kg −1 DW in the leaves, when treated with 30 mmol Cu l −1 (2000 mg l −1 ). Quantum efficiency of PSII, net photosynthesis rate, as well as leaf Ca and Mg concentrations were all negatively affected by Cu concentrations greater than 9 mmol l −1 in the nutrient solution. Our results indicate that the reduction in photosynthetic performance may be attributed to the adverse effect of excess Cu on the photosynthetic apparatus of the plant, both directly, via a decrease in pigment concentrations, and indirectly, via interference of Cu with Ca ions of PSII. Growth and seed production were only slightly affected by leaf tissue concentrations as high as 230 mg Cu kg −1 dry mass, which suggests that this species could play an important role in phytoremediation of Cu-contaminated soils.

  • physiological responses to salinity in the yellow horned poppy Glaucium flavum
    Plant Physiology and Biochemistry, 2011
    Co-Authors: J Cambrolle, T Luque, Susana Redondogomez, Enrique Mateosnaranjo, M E Figueroa
    Abstract:

    Abstract Glaucium flavum Crantz. is a short-lived perennial herb found in coastal habitats in southern Spain growing under a wide range of interstitial soil salinity levels, from that of fresh water up to the high concentration typical of sea water. An experiment was designed to investigate the effect of exposure to this range of salinity on the photosynthetic apparatus, growth and reproduction of G. flavum , by measuring relative growth rate, percentage of dead leaves, seed production, leaf relative water content, chlorophyll fluorescence parameters, gas exchange and photosynthetic pigment concentrations. We also determined total sodium, potassium, calcium, magnesium, and nitrogen concentrations. G. flavum survived at NaCl concentrations as high as 300 mM, although the excess of NaCl resulted in a biomass reduction of between 26 and 76% (in 60 and 300 mM NaCl treatments, respectively). The long-term effects of salinity on the growth and reproduction of G. flavum were mainly linked to an overall reduction in carbon gain as a result of stomatal conductance regulation. Also, the excess of salt caused a reduction in pigment concentrations, as well as Ca-, Mg- and N-uptake. The results indicate that, in the presence of excess soil-water salinity, G. flavum sustains little overall effects on the photochemical (PSII) apparatus, and is capable of tolerating a very high and continued exposure to salinity by maintaining low levels of net photosynthesis.

Ivan Svinyarov - One of the best experts on this subject based on the ideXlab platform.

  • Ionic Liquid-Based Aqueous Biphasic Systems—A Facile Approach for Ionic Liquid Regeneration from Crude Plant Extracts
    Processes, 2015
    Co-Authors: Rozalina Keremedchieva, Ivan Svinyarov, Milen G. Bogdanov
    Abstract:

    The possibility for simultaneous controlled partition of the biologically active alkaloid S-(+)-glaucine, presented in a crude ionic liquid-aqueous plant extract, and separation of the ionic liquid (IL) employed by means of ionic liquid-based aqueous biphasic systems (IL-ABS) was investigated in this study. The ABS were formed by the addition of inorganic salts with distinct water-structuring properties such as Na2CO3, MgSO4, (NH4)2SO4, and NaH2PO4 to an enriched of glaucine extract of Glaucium flavum Cr. (Papaveraceae). The influence of the salt type and concentration on the phase-forming ability, as well as the pH value on the partition of glaucine between the two phases formed, was comprehensively studied. It was found that the target alkaloid is predominantly transferred into the IL-rich phase, regardless the influential factors. The results obtained were further used as a platform for the development of an improved extractive procedure, ensuring simultaneous glaucine recovery, IL recycling and water removal in a single technological step. Thus, based on the formation of a three-phase system consisting of butyl acetate, IL-rich phase, and salt-rich phase, nearly quantitative recovery of glaucine (>99%), IL recycling (ca. 90%), and water removal (ca. 85%) were achieved by salt-induced separation of the crude plant extract.

  • ionic liquid supported solid liquid extraction of bioactive alkaloids iii ionic liquid regeneration and glaucine recovery from ionic liquid aqueous crude extract of Glaucium flavum cr papaveraceae
    Separation and Purification Technology, 2015
    Co-Authors: Milen G. Bogdanov, Rozalina Keremedchieva, Ivan Svinyarov
    Abstract:

    Abstract In continuation of a research project aiming at introducing ionic liquids (ILs) as an alternative to the widely applied for the recovery of natural products of industrial interest conventional molecular solvents, we developed a concise procedure for isolation of the biologically active alkaloid S -(+)-glaucine from IL-based aqueous crude extract. To this end, a comparative study of the behavior of 1 M [C 4 C 1 im][Ace]-aqueous solution and methanol in a series of consecutive extractions with the same extractant was conducted. The results obtained proved the better performance of the IL-based system in the solid–liquid extraction step, since the latter showed constantly higher extraction efficiency (ca. 35% enhanced) compared to methanol. The above procedure allows glaucine accumulation from at least ten successive extractions, while simultaneously reduces the total solid–liquid ratio from 1:40 to 1:7.2, without loss of efficiency. Furthermore, the loss of IL into the matrix pores after extraction was also considered, suggesting the need for IL recycling by posttreatment of the residual biomass. To recover glaucine from the crude IL-based aqueous extract, a series of non-miscible with water molecular solvents were tested. As a result, optimal conditions for quantitative extraction into chloroform were found, from which, after solvent removal and subsequent crystallization from ethanol, the target compound was isolated as a hydrobromide salt, the latter being the marketed form of glaucine.

  • Ionic liquid-supported solid–liquid extraction of bioactive alkaloids. III. Ionic liquid regeneration and glaucine recovery from ionic liquid-aqueous crude extract of Glaucium flavum Cr. (Papaveraceae)
    Separation and Purification Technology, 2015
    Co-Authors: Milen G. Bogdanov, Rozalina Keremedchieva, Ivan Svinyarov
    Abstract:

    Abstract In continuation of a research project aiming at introducing ionic liquids (ILs) as an alternative to the widely applied for the recovery of natural products of industrial interest conventional molecular solvents, we developed a concise procedure for isolation of the biologically active alkaloid S -(+)-glaucine from IL-based aqueous crude extract. To this end, a comparative study of the behavior of 1 M [C 4 C 1 im][Ace]-aqueous solution and methanol in a series of consecutive extractions with the same extractant was conducted. The results obtained proved the better performance of the IL-based system in the solid–liquid extraction step, since the latter showed constantly higher extraction efficiency (ca. 35% enhanced) compared to methanol. The above procedure allows glaucine accumulation from at least ten successive extractions, while simultaneously reduces the total solid–liquid ratio from 1:40 to 1:7.2, without loss of efficiency. Furthermore, the loss of IL into the matrix pores after extraction was also considered, suggesting the need for IL recycling by posttreatment of the residual biomass. To recover glaucine from the crude IL-based aqueous extract, a series of non-miscible with water molecular solvents were tested. As a result, optimal conditions for quantitative extraction into chloroform were found, from which, after solvent removal and subsequent crystallization from ethanol, the target compound was isolated as a hydrobromide salt, the latter being the marketed form of glaucine.

  • ionic liquid supported solid liquid extraction of bioactive alkaloids ii kinetics modeling and mechanism of glaucine extraction from Glaucium flavum cr papaveraceae
    Separation and Purification Technology, 2013
    Co-Authors: Milen G. Bogdanov, Ivan Svinyarov
    Abstract:

    Abstract The temperature dependence on the global kinetics of both methanol and 1-butyl-3-methylimidazolium acesulfamate ([C4mim][Ace])-supported extraction of the bioactive alkaloid S-(+)-glaucine from plant material of Glaucium flavum Crantz (Papaveraceae) was measured and a comparative analysis in respect to the extractant type was performed. The experimental data was fitted with high coefficients of determination by means of an empirical model showing that the extraction process in both cases follows second-order kinetics. The concentration at saturation, Cs, extraction rate constants, k2, initial rates of extraction, h, and activation energies of extraction, Ea, were then calculated and discussed. The obtained results give a considerable advantage to the Ionic liquid-supported (IL-supported) extraction procedure due to the faster and quantitative glaucine transfer, regardless the temperature. A plausible extraction mechanism scenario, describing the whole system in terms of solute–solvent, solute–matrix and matrix–solvent interactions at every stage of the extraction process was proposed. As a result, the apparent kinetics and yield of the IL-supported extraction were attributed to the cell wall modification by H-bonding interactions between both ions of [C4mim][Ace] and cellulose, resulting in the plant tissues disruption. The latter was further unequivocally confirmed by means of scanning electron microscopy. The kinetics parameters obtained were then implemented into a model for the prediction of glaucine amount extracted in 1 M [C4mim][Ace] water solution at any temperature and time with good predictive ability.

  • Ionic liquid-supported solid–liquid extraction of bioactive alkaloids. II. Kinetics, modeling and mechanism of glaucine extraction from Glaucium flavum Cr. (Papaveraceae)
    Separation and Purification Technology, 2013
    Co-Authors: Milen G. Bogdanov, Ivan Svinyarov
    Abstract:

    Abstract The temperature dependence on the global kinetics of both methanol and 1-butyl-3-methylimidazolium acesulfamate ([C4mim][Ace])-supported extraction of the bioactive alkaloid S-(+)-glaucine from plant material of Glaucium flavum Crantz (Papaveraceae) was measured and a comparative analysis in respect to the extractant type was performed. The experimental data was fitted with high coefficients of determination by means of an empirical model showing that the extraction process in both cases follows second-order kinetics. The concentration at saturation, Cs, extraction rate constants, k2, initial rates of extraction, h, and activation energies of extraction, Ea, were then calculated and discussed. The obtained results give a considerable advantage to the Ionic liquid-supported (IL-supported) extraction procedure due to the faster and quantitative glaucine transfer, regardless the temperature. A plausible extraction mechanism scenario, describing the whole system in terms of solute–solvent, solute–matrix and matrix–solvent interactions at every stage of the extraction process was proposed. As a result, the apparent kinetics and yield of the IL-supported extraction were attributed to the cell wall modification by H-bonding interactions between both ions of [C4mim][Ace] and cellulose, resulting in the plant tissues disruption. The latter was further unequivocally confirmed by means of scanning electron microscopy. The kinetics parameters obtained were then implemented into a model for the prediction of glaucine amount extracted in 1 M [C4mim][Ace] water solution at any temperature and time with good predictive ability.

Milen G. Bogdanov - One of the best experts on this subject based on the ideXlab platform.

  • Ionic Liquid-Based Aqueous Biphasic Systems—A Facile Approach for Ionic Liquid Regeneration from Crude Plant Extracts
    Processes, 2015
    Co-Authors: Rozalina Keremedchieva, Ivan Svinyarov, Milen G. Bogdanov
    Abstract:

    The possibility for simultaneous controlled partition of the biologically active alkaloid S-(+)-glaucine, presented in a crude ionic liquid-aqueous plant extract, and separation of the ionic liquid (IL) employed by means of ionic liquid-based aqueous biphasic systems (IL-ABS) was investigated in this study. The ABS were formed by the addition of inorganic salts with distinct water-structuring properties such as Na2CO3, MgSO4, (NH4)2SO4, and NaH2PO4 to an enriched of glaucine extract of Glaucium flavum Cr. (Papaveraceae). The influence of the salt type and concentration on the phase-forming ability, as well as the pH value on the partition of glaucine between the two phases formed, was comprehensively studied. It was found that the target alkaloid is predominantly transferred into the IL-rich phase, regardless the influential factors. The results obtained were further used as a platform for the development of an improved extractive procedure, ensuring simultaneous glaucine recovery, IL recycling and water removal in a single technological step. Thus, based on the formation of a three-phase system consisting of butyl acetate, IL-rich phase, and salt-rich phase, nearly quantitative recovery of glaucine (>99%), IL recycling (ca. 90%), and water removal (ca. 85%) were achieved by salt-induced separation of the crude plant extract.

  • ionic liquid supported solid liquid extraction of bioactive alkaloids iii ionic liquid regeneration and glaucine recovery from ionic liquid aqueous crude extract of Glaucium flavum cr papaveraceae
    Separation and Purification Technology, 2015
    Co-Authors: Milen G. Bogdanov, Rozalina Keremedchieva, Ivan Svinyarov
    Abstract:

    Abstract In continuation of a research project aiming at introducing ionic liquids (ILs) as an alternative to the widely applied for the recovery of natural products of industrial interest conventional molecular solvents, we developed a concise procedure for isolation of the biologically active alkaloid S -(+)-glaucine from IL-based aqueous crude extract. To this end, a comparative study of the behavior of 1 M [C 4 C 1 im][Ace]-aqueous solution and methanol in a series of consecutive extractions with the same extractant was conducted. The results obtained proved the better performance of the IL-based system in the solid–liquid extraction step, since the latter showed constantly higher extraction efficiency (ca. 35% enhanced) compared to methanol. The above procedure allows glaucine accumulation from at least ten successive extractions, while simultaneously reduces the total solid–liquid ratio from 1:40 to 1:7.2, without loss of efficiency. Furthermore, the loss of IL into the matrix pores after extraction was also considered, suggesting the need for IL recycling by posttreatment of the residual biomass. To recover glaucine from the crude IL-based aqueous extract, a series of non-miscible with water molecular solvents were tested. As a result, optimal conditions for quantitative extraction into chloroform were found, from which, after solvent removal and subsequent crystallization from ethanol, the target compound was isolated as a hydrobromide salt, the latter being the marketed form of glaucine.

  • Ionic liquid-supported solid–liquid extraction of bioactive alkaloids. III. Ionic liquid regeneration and glaucine recovery from ionic liquid-aqueous crude extract of Glaucium flavum Cr. (Papaveraceae)
    Separation and Purification Technology, 2015
    Co-Authors: Milen G. Bogdanov, Rozalina Keremedchieva, Ivan Svinyarov
    Abstract:

    Abstract In continuation of a research project aiming at introducing ionic liquids (ILs) as an alternative to the widely applied for the recovery of natural products of industrial interest conventional molecular solvents, we developed a concise procedure for isolation of the biologically active alkaloid S -(+)-glaucine from IL-based aqueous crude extract. To this end, a comparative study of the behavior of 1 M [C 4 C 1 im][Ace]-aqueous solution and methanol in a series of consecutive extractions with the same extractant was conducted. The results obtained proved the better performance of the IL-based system in the solid–liquid extraction step, since the latter showed constantly higher extraction efficiency (ca. 35% enhanced) compared to methanol. The above procedure allows glaucine accumulation from at least ten successive extractions, while simultaneously reduces the total solid–liquid ratio from 1:40 to 1:7.2, without loss of efficiency. Furthermore, the loss of IL into the matrix pores after extraction was also considered, suggesting the need for IL recycling by posttreatment of the residual biomass. To recover glaucine from the crude IL-based aqueous extract, a series of non-miscible with water molecular solvents were tested. As a result, optimal conditions for quantitative extraction into chloroform were found, from which, after solvent removal and subsequent crystallization from ethanol, the target compound was isolated as a hydrobromide salt, the latter being the marketed form of glaucine.

  • ionic liquid supported solid liquid extraction of bioactive alkaloids ii kinetics modeling and mechanism of glaucine extraction from Glaucium flavum cr papaveraceae
    Separation and Purification Technology, 2013
    Co-Authors: Milen G. Bogdanov, Ivan Svinyarov
    Abstract:

    Abstract The temperature dependence on the global kinetics of both methanol and 1-butyl-3-methylimidazolium acesulfamate ([C4mim][Ace])-supported extraction of the bioactive alkaloid S-(+)-glaucine from plant material of Glaucium flavum Crantz (Papaveraceae) was measured and a comparative analysis in respect to the extractant type was performed. The experimental data was fitted with high coefficients of determination by means of an empirical model showing that the extraction process in both cases follows second-order kinetics. The concentration at saturation, Cs, extraction rate constants, k2, initial rates of extraction, h, and activation energies of extraction, Ea, were then calculated and discussed. The obtained results give a considerable advantage to the Ionic liquid-supported (IL-supported) extraction procedure due to the faster and quantitative glaucine transfer, regardless the temperature. A plausible extraction mechanism scenario, describing the whole system in terms of solute–solvent, solute–matrix and matrix–solvent interactions at every stage of the extraction process was proposed. As a result, the apparent kinetics and yield of the IL-supported extraction were attributed to the cell wall modification by H-bonding interactions between both ions of [C4mim][Ace] and cellulose, resulting in the plant tissues disruption. The latter was further unequivocally confirmed by means of scanning electron microscopy. The kinetics parameters obtained were then implemented into a model for the prediction of glaucine amount extracted in 1 M [C4mim][Ace] water solution at any temperature and time with good predictive ability.

  • Ionic liquid-supported solid–liquid extraction of bioactive alkaloids. II. Kinetics, modeling and mechanism of glaucine extraction from Glaucium flavum Cr. (Papaveraceae)
    Separation and Purification Technology, 2013
    Co-Authors: Milen G. Bogdanov, Ivan Svinyarov
    Abstract:

    Abstract The temperature dependence on the global kinetics of both methanol and 1-butyl-3-methylimidazolium acesulfamate ([C4mim][Ace])-supported extraction of the bioactive alkaloid S-(+)-glaucine from plant material of Glaucium flavum Crantz (Papaveraceae) was measured and a comparative analysis in respect to the extractant type was performed. The experimental data was fitted with high coefficients of determination by means of an empirical model showing that the extraction process in both cases follows second-order kinetics. The concentration at saturation, Cs, extraction rate constants, k2, initial rates of extraction, h, and activation energies of extraction, Ea, were then calculated and discussed. The obtained results give a considerable advantage to the Ionic liquid-supported (IL-supported) extraction procedure due to the faster and quantitative glaucine transfer, regardless the temperature. A plausible extraction mechanism scenario, describing the whole system in terms of solute–solvent, solute–matrix and matrix–solvent interactions at every stage of the extraction process was proposed. As a result, the apparent kinetics and yield of the IL-supported extraction were attributed to the cell wall modification by H-bonding interactions between both ions of [C4mim][Ace] and cellulose, resulting in the plant tissues disruption. The latter was further unequivocally confirmed by means of scanning electron microscopy. The kinetics parameters obtained were then implemented into a model for the prediction of glaucine amount extracted in 1 M [C4mim][Ace] water solution at any temperature and time with good predictive ability.