The Experts below are selected from a list of 51 Experts worldwide ranked by ideXlab platform

Marcelo Lima Ribeiro - One of the best experts on this subject based on the ideXlab platform.

  • modulatory effects of yerba mate ilex paraguariensis on the pi3k akt signaling pathway
    Molecular Nutrition & Food Research, 2013
    Co-Authors: Alessandra Gambero, Marcelo Lima Ribeiro, Demetrius Paiva Arcari, Juliana Carvalho Santos, Lucio Fabio Caldas Ferraz
    Abstract:

    The aim of this study was to evaluate the effects of yerba mate (YM) extract on the phosphatidylinositol 3-kinase (PI3K)-AKT signaling pathway in vivo. The mice were introduced to either standard- or high-fat diet (HFD). After 8 weeks on an HFD, mice were randomly assigned to one of the two treatment conditions, water or yerba mate extract at 1.0 g/kg. After treatment, Glucose Blood Level and hepatic insulin response were evaluated. Liver tissue was examined to determine the mRNA Levels using the PI3K-AKT PCR array. The nuclear translocation of forkhead box O1 (FOXO1) was determined by an electrophoretic mobility-shift assay. Our data demonstrated that yerba mate extract significantly decreased the final body weight, Glucose Blood Levels, and insulin resistance of mice. Molecular analysis demonstrated that an HFD downregulated Akt2, Irs1, Irs2, Pi3kca, Pi3kcg, and Pdk1; after yerba mate treatment, the Levels of those genes returned to baseline. In addition, an HFD upregulated Pepck and G6pc and increased FOXO1 nuclear translocation. The intervention downregulated these genes by decreasing FOXO1 nuclear translocation. The results obtained demonstrate for the first time the specific action of yerba mate on the PI3K-AKT pathway, which contributed to the observed improvement in hepatic insulin signaling.

  • Modulatory effects of yerba maté (Ilex paraguariensis) on the PI3K-AKT signaling pathway.
    Molecular nutrition & food research, 2013
    Co-Authors: Demetrius Paiva Arcari, Alessandra Gambero, Juliana Carvalho Santos, Lucio Fabio Caldas Ferraz, Marcelo Lima Ribeiro
    Abstract:

    The aim of this study was to evaluate the effects of yerba maté (YM) extract on the phosphatidylinositol 3-kinase (PI3K)-AKT signaling pathway in vivo. The mice were introduced to either standard- or high-fat diet (HFD). After 8 weeks on an HFD, mice were randomly assigned to one of the two treatment conditions, water or yerba maté extract at 1.0 g/kg. After treatment, Glucose Blood Level and hepatic insulin response were evaluated. Liver tissue was examined to determine the mRNA Levels using the PI3K-AKT PCR array. The nuclear translocation of forkhead box O1 (FOXO1) was determined by an electrophoretic mobility-shift assay. Our data demonstrated that yerba maté extract significantly decreased the final body weight, Glucose Blood Levels, and insulin resistance of mice. Molecular analysis demonstrated that an HFD downregulated Akt2, Irs1, Irs2, Pi3kca, Pi3kcg, and Pdk1; after yerba maté treatment, the Levels of those genes returned to baseline. In addition, an HFD upregulated Pepck and G6pc and increased FOXO1 nuclear translocation. The intervention downregulated these genes by decreasing FOXO1 nuclear translocation. The results obtained demonstrate for the first time the specific action of yerba maté on the PI3K-AKT pathway, which contributed to the observed improvement in hepatic insulin signaling.

Demetrius Paiva Arcari - One of the best experts on this subject based on the ideXlab platform.

  • modulatory effects of yerba mate ilex paraguariensis on the pi3k akt signaling pathway
    Molecular Nutrition & Food Research, 2013
    Co-Authors: Alessandra Gambero, Marcelo Lima Ribeiro, Demetrius Paiva Arcari, Juliana Carvalho Santos, Lucio Fabio Caldas Ferraz
    Abstract:

    The aim of this study was to evaluate the effects of yerba mate (YM) extract on the phosphatidylinositol 3-kinase (PI3K)-AKT signaling pathway in vivo. The mice were introduced to either standard- or high-fat diet (HFD). After 8 weeks on an HFD, mice were randomly assigned to one of the two treatment conditions, water or yerba mate extract at 1.0 g/kg. After treatment, Glucose Blood Level and hepatic insulin response were evaluated. Liver tissue was examined to determine the mRNA Levels using the PI3K-AKT PCR array. The nuclear translocation of forkhead box O1 (FOXO1) was determined by an electrophoretic mobility-shift assay. Our data demonstrated that yerba mate extract significantly decreased the final body weight, Glucose Blood Levels, and insulin resistance of mice. Molecular analysis demonstrated that an HFD downregulated Akt2, Irs1, Irs2, Pi3kca, Pi3kcg, and Pdk1; after yerba mate treatment, the Levels of those genes returned to baseline. In addition, an HFD upregulated Pepck and G6pc and increased FOXO1 nuclear translocation. The intervention downregulated these genes by decreasing FOXO1 nuclear translocation. The results obtained demonstrate for the first time the specific action of yerba mate on the PI3K-AKT pathway, which contributed to the observed improvement in hepatic insulin signaling.

  • Modulatory effects of yerba maté (Ilex paraguariensis) on the PI3K-AKT signaling pathway.
    Molecular nutrition & food research, 2013
    Co-Authors: Demetrius Paiva Arcari, Alessandra Gambero, Juliana Carvalho Santos, Lucio Fabio Caldas Ferraz, Marcelo Lima Ribeiro
    Abstract:

    The aim of this study was to evaluate the effects of yerba maté (YM) extract on the phosphatidylinositol 3-kinase (PI3K)-AKT signaling pathway in vivo. The mice were introduced to either standard- or high-fat diet (HFD). After 8 weeks on an HFD, mice were randomly assigned to one of the two treatment conditions, water or yerba maté extract at 1.0 g/kg. After treatment, Glucose Blood Level and hepatic insulin response were evaluated. Liver tissue was examined to determine the mRNA Levels using the PI3K-AKT PCR array. The nuclear translocation of forkhead box O1 (FOXO1) was determined by an electrophoretic mobility-shift assay. Our data demonstrated that yerba maté extract significantly decreased the final body weight, Glucose Blood Levels, and insulin resistance of mice. Molecular analysis demonstrated that an HFD downregulated Akt2, Irs1, Irs2, Pi3kca, Pi3kcg, and Pdk1; after yerba maté treatment, the Levels of those genes returned to baseline. In addition, an HFD upregulated Pepck and G6pc and increased FOXO1 nuclear translocation. The intervention downregulated these genes by decreasing FOXO1 nuclear translocation. The results obtained demonstrate for the first time the specific action of yerba maté on the PI3K-AKT pathway, which contributed to the observed improvement in hepatic insulin signaling.

  • anti inflammatory effects of yerba mate extract ilex paraguariensis ameliorate insulin resistance in mice with high fat diet induced obesity
    Molecular and Cellular Endocrinology, 2011
    Co-Authors: Demetrius Paiva Arcari, Alessandra Gambero, Lucio Fabio Caldas Ferraz, Waldemar Bartchewsky, Tanila Wood Dos Santos, Karim A Oliveira, Carlorine C Deoliveira, Erica Martins Ferreira Gotardo, Jose Pedrazzoli, Patricia De Oliveira Carvalho
    Abstract:

    The aim of the present study was to evaluate the effects of yerba mate extract upon markers of insulin resistance and inflammatory markers in mice with high fat diet-induced obesity. The mice were introduced to either standard or high fat diets. After 12 weeks on a high fat diet, mice were randomly assigned to one of the two treatment conditions, water or yerba mate extract at 1.0 gkg(-1). After treatment, Glucose Blood Level and hepatic and soleus muscle insulin response were evaluated. Serum Levels of TNF-α and IL-6 were evaluated by ELISA, liver tissue was examined to determine the mRNA Levels of TNF-α, IL-6 and iNOS, and the nuclear translocation of NF-κB was determined by an electrophoretic mobility shift assay. Our data show improvements in both the basal Glucose Blood Levels and in the response to insulin administration in the treated animals. The molecular analysis of insulin signalling revealed a restoration of hepatic and muscle insulin substrate receptor (IRS)-1 and AKT phosphorylation. Our data show that the high fat diet caused an up-regulation of the TNF-α, IL-6, and iNOS genes. Although after intervention with yerba mate extract the expression Levels of those genes returned to baseline through the NF-κB pathway, these results could also be secondary to the weight loss observed. In conclusion, our results indicate that yerba mate has a potential anti-inflammatory effect. Additionally, these data demonstrate that yerba mate inhibits hepatic and muscle TNF-α and restores hepatic insulin signalling in mice with high fat diet-induced obesity.

Juliana Carvalho Santos - One of the best experts on this subject based on the ideXlab platform.

  • modulatory effects of yerba mate ilex paraguariensis on the pi3k akt signaling pathway
    Molecular Nutrition & Food Research, 2013
    Co-Authors: Alessandra Gambero, Marcelo Lima Ribeiro, Demetrius Paiva Arcari, Juliana Carvalho Santos, Lucio Fabio Caldas Ferraz
    Abstract:

    The aim of this study was to evaluate the effects of yerba mate (YM) extract on the phosphatidylinositol 3-kinase (PI3K)-AKT signaling pathway in vivo. The mice were introduced to either standard- or high-fat diet (HFD). After 8 weeks on an HFD, mice were randomly assigned to one of the two treatment conditions, water or yerba mate extract at 1.0 g/kg. After treatment, Glucose Blood Level and hepatic insulin response were evaluated. Liver tissue was examined to determine the mRNA Levels using the PI3K-AKT PCR array. The nuclear translocation of forkhead box O1 (FOXO1) was determined by an electrophoretic mobility-shift assay. Our data demonstrated that yerba mate extract significantly decreased the final body weight, Glucose Blood Levels, and insulin resistance of mice. Molecular analysis demonstrated that an HFD downregulated Akt2, Irs1, Irs2, Pi3kca, Pi3kcg, and Pdk1; after yerba mate treatment, the Levels of those genes returned to baseline. In addition, an HFD upregulated Pepck and G6pc and increased FOXO1 nuclear translocation. The intervention downregulated these genes by decreasing FOXO1 nuclear translocation. The results obtained demonstrate for the first time the specific action of yerba mate on the PI3K-AKT pathway, which contributed to the observed improvement in hepatic insulin signaling.

  • Modulatory effects of yerba maté (Ilex paraguariensis) on the PI3K-AKT signaling pathway.
    Molecular nutrition & food research, 2013
    Co-Authors: Demetrius Paiva Arcari, Alessandra Gambero, Juliana Carvalho Santos, Lucio Fabio Caldas Ferraz, Marcelo Lima Ribeiro
    Abstract:

    The aim of this study was to evaluate the effects of yerba maté (YM) extract on the phosphatidylinositol 3-kinase (PI3K)-AKT signaling pathway in vivo. The mice were introduced to either standard- or high-fat diet (HFD). After 8 weeks on an HFD, mice were randomly assigned to one of the two treatment conditions, water or yerba maté extract at 1.0 g/kg. After treatment, Glucose Blood Level and hepatic insulin response were evaluated. Liver tissue was examined to determine the mRNA Levels using the PI3K-AKT PCR array. The nuclear translocation of forkhead box O1 (FOXO1) was determined by an electrophoretic mobility-shift assay. Our data demonstrated that yerba maté extract significantly decreased the final body weight, Glucose Blood Levels, and insulin resistance of mice. Molecular analysis demonstrated that an HFD downregulated Akt2, Irs1, Irs2, Pi3kca, Pi3kcg, and Pdk1; after yerba maté treatment, the Levels of those genes returned to baseline. In addition, an HFD upregulated Pepck and G6pc and increased FOXO1 nuclear translocation. The intervention downregulated these genes by decreasing FOXO1 nuclear translocation. The results obtained demonstrate for the first time the specific action of yerba maté on the PI3K-AKT pathway, which contributed to the observed improvement in hepatic insulin signaling.

Alessandra Gambero - One of the best experts on this subject based on the ideXlab platform.

  • modulatory effects of yerba mate ilex paraguariensis on the pi3k akt signaling pathway
    Molecular Nutrition & Food Research, 2013
    Co-Authors: Alessandra Gambero, Marcelo Lima Ribeiro, Demetrius Paiva Arcari, Juliana Carvalho Santos, Lucio Fabio Caldas Ferraz
    Abstract:

    The aim of this study was to evaluate the effects of yerba mate (YM) extract on the phosphatidylinositol 3-kinase (PI3K)-AKT signaling pathway in vivo. The mice were introduced to either standard- or high-fat diet (HFD). After 8 weeks on an HFD, mice were randomly assigned to one of the two treatment conditions, water or yerba mate extract at 1.0 g/kg. After treatment, Glucose Blood Level and hepatic insulin response were evaluated. Liver tissue was examined to determine the mRNA Levels using the PI3K-AKT PCR array. The nuclear translocation of forkhead box O1 (FOXO1) was determined by an electrophoretic mobility-shift assay. Our data demonstrated that yerba mate extract significantly decreased the final body weight, Glucose Blood Levels, and insulin resistance of mice. Molecular analysis demonstrated that an HFD downregulated Akt2, Irs1, Irs2, Pi3kca, Pi3kcg, and Pdk1; after yerba mate treatment, the Levels of those genes returned to baseline. In addition, an HFD upregulated Pepck and G6pc and increased FOXO1 nuclear translocation. The intervention downregulated these genes by decreasing FOXO1 nuclear translocation. The results obtained demonstrate for the first time the specific action of yerba mate on the PI3K-AKT pathway, which contributed to the observed improvement in hepatic insulin signaling.

  • Modulatory effects of yerba maté (Ilex paraguariensis) on the PI3K-AKT signaling pathway.
    Molecular nutrition & food research, 2013
    Co-Authors: Demetrius Paiva Arcari, Alessandra Gambero, Juliana Carvalho Santos, Lucio Fabio Caldas Ferraz, Marcelo Lima Ribeiro
    Abstract:

    The aim of this study was to evaluate the effects of yerba maté (YM) extract on the phosphatidylinositol 3-kinase (PI3K)-AKT signaling pathway in vivo. The mice were introduced to either standard- or high-fat diet (HFD). After 8 weeks on an HFD, mice were randomly assigned to one of the two treatment conditions, water or yerba maté extract at 1.0 g/kg. After treatment, Glucose Blood Level and hepatic insulin response were evaluated. Liver tissue was examined to determine the mRNA Levels using the PI3K-AKT PCR array. The nuclear translocation of forkhead box O1 (FOXO1) was determined by an electrophoretic mobility-shift assay. Our data demonstrated that yerba maté extract significantly decreased the final body weight, Glucose Blood Levels, and insulin resistance of mice. Molecular analysis demonstrated that an HFD downregulated Akt2, Irs1, Irs2, Pi3kca, Pi3kcg, and Pdk1; after yerba maté treatment, the Levels of those genes returned to baseline. In addition, an HFD upregulated Pepck and G6pc and increased FOXO1 nuclear translocation. The intervention downregulated these genes by decreasing FOXO1 nuclear translocation. The results obtained demonstrate for the first time the specific action of yerba maté on the PI3K-AKT pathway, which contributed to the observed improvement in hepatic insulin signaling.

  • anti inflammatory effects of yerba mate extract ilex paraguariensis ameliorate insulin resistance in mice with high fat diet induced obesity
    Molecular and Cellular Endocrinology, 2011
    Co-Authors: Demetrius Paiva Arcari, Alessandra Gambero, Lucio Fabio Caldas Ferraz, Waldemar Bartchewsky, Tanila Wood Dos Santos, Karim A Oliveira, Carlorine C Deoliveira, Erica Martins Ferreira Gotardo, Jose Pedrazzoli, Patricia De Oliveira Carvalho
    Abstract:

    The aim of the present study was to evaluate the effects of yerba mate extract upon markers of insulin resistance and inflammatory markers in mice with high fat diet-induced obesity. The mice were introduced to either standard or high fat diets. After 12 weeks on a high fat diet, mice were randomly assigned to one of the two treatment conditions, water or yerba mate extract at 1.0 gkg(-1). After treatment, Glucose Blood Level and hepatic and soleus muscle insulin response were evaluated. Serum Levels of TNF-α and IL-6 were evaluated by ELISA, liver tissue was examined to determine the mRNA Levels of TNF-α, IL-6 and iNOS, and the nuclear translocation of NF-κB was determined by an electrophoretic mobility shift assay. Our data show improvements in both the basal Glucose Blood Levels and in the response to insulin administration in the treated animals. The molecular analysis of insulin signalling revealed a restoration of hepatic and muscle insulin substrate receptor (IRS)-1 and AKT phosphorylation. Our data show that the high fat diet caused an up-regulation of the TNF-α, IL-6, and iNOS genes. Although after intervention with yerba mate extract the expression Levels of those genes returned to baseline through the NF-κB pathway, these results could also be secondary to the weight loss observed. In conclusion, our results indicate that yerba mate has a potential anti-inflammatory effect. Additionally, these data demonstrate that yerba mate inhibits hepatic and muscle TNF-α and restores hepatic insulin signalling in mice with high fat diet-induced obesity.

Lucio Fabio Caldas Ferraz - One of the best experts on this subject based on the ideXlab platform.

  • modulatory effects of yerba mate ilex paraguariensis on the pi3k akt signaling pathway
    Molecular Nutrition & Food Research, 2013
    Co-Authors: Alessandra Gambero, Marcelo Lima Ribeiro, Demetrius Paiva Arcari, Juliana Carvalho Santos, Lucio Fabio Caldas Ferraz
    Abstract:

    The aim of this study was to evaluate the effects of yerba mate (YM) extract on the phosphatidylinositol 3-kinase (PI3K)-AKT signaling pathway in vivo. The mice were introduced to either standard- or high-fat diet (HFD). After 8 weeks on an HFD, mice were randomly assigned to one of the two treatment conditions, water or yerba mate extract at 1.0 g/kg. After treatment, Glucose Blood Level and hepatic insulin response were evaluated. Liver tissue was examined to determine the mRNA Levels using the PI3K-AKT PCR array. The nuclear translocation of forkhead box O1 (FOXO1) was determined by an electrophoretic mobility-shift assay. Our data demonstrated that yerba mate extract significantly decreased the final body weight, Glucose Blood Levels, and insulin resistance of mice. Molecular analysis demonstrated that an HFD downregulated Akt2, Irs1, Irs2, Pi3kca, Pi3kcg, and Pdk1; after yerba mate treatment, the Levels of those genes returned to baseline. In addition, an HFD upregulated Pepck and G6pc and increased FOXO1 nuclear translocation. The intervention downregulated these genes by decreasing FOXO1 nuclear translocation. The results obtained demonstrate for the first time the specific action of yerba mate on the PI3K-AKT pathway, which contributed to the observed improvement in hepatic insulin signaling.

  • Modulatory effects of yerba maté (Ilex paraguariensis) on the PI3K-AKT signaling pathway.
    Molecular nutrition & food research, 2013
    Co-Authors: Demetrius Paiva Arcari, Alessandra Gambero, Juliana Carvalho Santos, Lucio Fabio Caldas Ferraz, Marcelo Lima Ribeiro
    Abstract:

    The aim of this study was to evaluate the effects of yerba maté (YM) extract on the phosphatidylinositol 3-kinase (PI3K)-AKT signaling pathway in vivo. The mice were introduced to either standard- or high-fat diet (HFD). After 8 weeks on an HFD, mice were randomly assigned to one of the two treatment conditions, water or yerba maté extract at 1.0 g/kg. After treatment, Glucose Blood Level and hepatic insulin response were evaluated. Liver tissue was examined to determine the mRNA Levels using the PI3K-AKT PCR array. The nuclear translocation of forkhead box O1 (FOXO1) was determined by an electrophoretic mobility-shift assay. Our data demonstrated that yerba maté extract significantly decreased the final body weight, Glucose Blood Levels, and insulin resistance of mice. Molecular analysis demonstrated that an HFD downregulated Akt2, Irs1, Irs2, Pi3kca, Pi3kcg, and Pdk1; after yerba maté treatment, the Levels of those genes returned to baseline. In addition, an HFD upregulated Pepck and G6pc and increased FOXO1 nuclear translocation. The intervention downregulated these genes by decreasing FOXO1 nuclear translocation. The results obtained demonstrate for the first time the specific action of yerba maté on the PI3K-AKT pathway, which contributed to the observed improvement in hepatic insulin signaling.

  • anti inflammatory effects of yerba mate extract ilex paraguariensis ameliorate insulin resistance in mice with high fat diet induced obesity
    Molecular and Cellular Endocrinology, 2011
    Co-Authors: Demetrius Paiva Arcari, Alessandra Gambero, Lucio Fabio Caldas Ferraz, Waldemar Bartchewsky, Tanila Wood Dos Santos, Karim A Oliveira, Carlorine C Deoliveira, Erica Martins Ferreira Gotardo, Jose Pedrazzoli, Patricia De Oliveira Carvalho
    Abstract:

    The aim of the present study was to evaluate the effects of yerba mate extract upon markers of insulin resistance and inflammatory markers in mice with high fat diet-induced obesity. The mice were introduced to either standard or high fat diets. After 12 weeks on a high fat diet, mice were randomly assigned to one of the two treatment conditions, water or yerba mate extract at 1.0 gkg(-1). After treatment, Glucose Blood Level and hepatic and soleus muscle insulin response were evaluated. Serum Levels of TNF-α and IL-6 were evaluated by ELISA, liver tissue was examined to determine the mRNA Levels of TNF-α, IL-6 and iNOS, and the nuclear translocation of NF-κB was determined by an electrophoretic mobility shift assay. Our data show improvements in both the basal Glucose Blood Levels and in the response to insulin administration in the treated animals. The molecular analysis of insulin signalling revealed a restoration of hepatic and muscle insulin substrate receptor (IRS)-1 and AKT phosphorylation. Our data show that the high fat diet caused an up-regulation of the TNF-α, IL-6, and iNOS genes. Although after intervention with yerba mate extract the expression Levels of those genes returned to baseline through the NF-κB pathway, these results could also be secondary to the weight loss observed. In conclusion, our results indicate that yerba mate has a potential anti-inflammatory effect. Additionally, these data demonstrate that yerba mate inhibits hepatic and muscle TNF-α and restores hepatic insulin signalling in mice with high fat diet-induced obesity.