The Experts below are selected from a list of 312 Experts worldwide ranked by ideXlab platform

Robert W Mahley - One of the best experts on this subject based on the ideXlab platform.

  • Glucuronic Acid epimerase is associated with plasma triglyceride and high density lipoprotein cholesterol levels in turks
    Annals of Human Genetics, 2011
    Co-Authors: Ugur Hodoglugil, David Williamson, Lindsay A Farrer, Robert W Mahley
    Abstract:

    We narrowed chromosome 15q21-23 linkage to plasma high-density lipoprotein cholesterol (HDL-C) levels in Turkish families by fine mapping, then focused on Glucuronic Acid epimerase (GLCE), a heparan sulfate proteoglycan (HSPG) biosynthesis enzyme. HSPGs participate in lipid metabolism along with apolipoprotein (apo) E. Of 31 SNPs in the GLCE locus, nine analyzed by haplotype were associated with HDL-C and triglyceride levels (permuted p = 0.006 and 0.013, respectively) in families. Of five tagging GLCE SNPs in two cohorts of unrelated subjects, three (rs16952868, rs11631403, and rs3865014) were associated with triglyceride and HDL-C levels in males (nonpermuted p < 0.05). The association was stronger in APOE 2/3 subjects (apoE2 has reduced binding to HSPGs) and reached multiple-testing significance (p < 0.05) in both males and females (n= 2612). Similar results were obtained in the second cohort (n= 1164). Interestingly, at the GLCE locus, bounded by recombination hotspots, Turks had a minor allele frequency of SNPs resembling Chinese more than European ancestry; adjoining regions resembled the European pattern. Studies of glce(+/-) apoe(-/-) mice fed a chow or high-fat diet supported a role for GLCE in lipid metabolism. Thus, SNPs in GLCE are associated with triglyceride and HDL-C levels in Turks, and mouse studies support a role for glce in lipid metabolism.

  • Glucuronic Acid epimerase is associated with plasma triglyceride and high density lipoprotein cholesterol levels in turks
    Annals of Human Genetics, 2011
    Co-Authors: Ugur Hodoglugil, David Williamson, Lindsay A Farrer, Yi Yu, Robert W Mahley
    Abstract:

    We narrowed chromosome 15q21-23 linkage to plasma high density lipoprotein cholesterol (HDL-C) levels in atherogenic dyslipidemic Turkish families by fine mapping, then focused on Glucuronic Acid epimerase (GLCE), a heparan sulfate proteoglycan (HSPG) biosynthesis enzyme. HSPGs participate in lipid metabolism along with apolipoprotein (apo) E. Of 31 SNPs in the GLCE locus, nine analyzed by haplotype were associated with plasma HDL-C and triglyceride levels (permuted p = 0.006 and 0.013, respectively) in families. Of five tagging GLCE SNPs in two cohorts of unrelated subjects, three (rs16952868, rs11631403, rs3865014) were associated with triglyceride and HDL-C levels in males (non-permuted p < 0.05). The association was stronger in APOE 2/3 subjects (apoE2 has reduced binding to HSPGs) and reached multiple-testing significance (p < 0.05) in both males and females (n = 2612). Similar results were obtained in the second cohort (n = 1164). Interestingly, at the GLCE locus, bounded by recombination hotspots, Turks had a minor allele frequency of SNPs resembling Chinese more than European ancestry; adjoining regions on chromosome 15 resembled the European pattern. Studies of glce+/–apoe–/– mice fed a chow or high-fat diet supported a role for GLCE in lipid metabolism. Thus, SNPs in GLCE are associated with triglyceride and HDL-C levels in Turks, and mouse studies support a role for glce in lipid metabolism.

Joong Kon Park - One of the best experts on this subject based on the ideXlab platform.

  • effects of Glucuronic Acid oligomers on the production structure and properties of bacterial cellulose
    Carbohydrate Polymers, 2013
    Co-Authors: Mazhar Ulislam, Taous Khan, Joong Kon Park
    Abstract:

    The addition of certain supplementary carbon sources to the culture media can influence the production, structural features and mechanical properties of bacterial cellulose (BC). In this study, different concentrations (0, 1, 2 and 4%) of a by-product, single sugar α-linked Glucuronic Acid-based oligosaccharide (SSGO), were added to the culture media during the production of BC. Production with 1% (BC1), 2% (BC2) and 4% (BC3) SSGO led to increases in BC production of 10.45, 12.74 and 9.01 g/L, respectively, after 10 days of cultivation under static conditions, while it was only 7.4 g/L when no SSGO was added (BC0). The structures of BC0, BC1, BC2, and BC3 were confirmed by XRD and FT-IR analysis. FE-SEM micrographs showed increased fibril thickness and decreased pore size in the SSGO added samples. The tensile strength of the BC0 was 16.73 MPa, while it was 25.05 MPa for BC1. However, with further increases in the concentration of SSGO, the tensile strength decreased to 20.76 and 19.77 MPa for BC2 and BC3, respectively. The results of this study provide further insight into the additive role of SSGO and improvement of the physico-mechanical properties of BC.

  • bacterial cellulose production from a single sugar α linked Glucuronic Acid based oligosaccharide
    Process Biochemistry, 2011
    Co-Authors: Nasrullah Shah, Taous Khan, Mazhar Ulislam, Joong Kon Park
    Abstract:

    Abstract A single sugar α-linked Glucuronic Acid-based oligosaccharide (SSGO), which is a by-product produced during bacterial cellulose (BC) synthesis by Gluconacetobacter hansenii , was evaluated as an additive to synthetic medium during BC production. We assessed the ability of the SSGO to serve as an alternative carbon source to glucose for BC production. To improve the BC yield from glucose by G. hansenii PJK, SSGO was added to a chemically defined medium (CDM). In the presence of 1% SSGO, the amount of BC produced was 10.5 g/L after 10 days of cultivation, whereas in the absence of SSGO, only 7.4 g/L of BC was produced. These results indicate that SSGO can be used as an alternative carbon source for BC production. Moreover, the addition of SSGO to the CDM increased the diameter of the BC fibers, which improved its tensile strength and water release properties.

  • the structure and physical properties of Glucuronic Acid oligomers produced by a gluconacetobacter hansenii strain using the waste from beer fermentation broth
    Carbohydrate Polymers, 2008
    Co-Authors: Taous Khan, Joong Kon Park
    Abstract:

    The aim of this work was to study the chemical structure and physical properties of water-soluble oligosaccharides (WSOS) produced by Gluconacetobacter hansenii PJK using the waste from beer fermentation broth as a basal medium. The analysis of the hydrolyzed products and the spectroscopic studies of the native WSOS showed that it is a mixture of oligomers all having a single sugar α-linked Glucuronic Acid as building blocks with an O-acetyl and O-methyl group, in the terminal unit of the non-reducing end. The thermal studies displayed a progressive degradation of WSOS without a major weight loss throughout a range of temperatures. The melting point and pyrolysis temperature were found to be 130.16 and 275.25 °C, respectively. The optimum concentration of WSOS for a maximum emulsifying ability was found to be 0.10% (w/v). The resulting emulsions, however, did not demonstrate a noteworthy stability.

  • structural studies of the Glucuronic Acid oligomers produced by gluconacetobacter hansenii strain
    Carbohydrate Polymers, 2006
    Co-Authors: Joong Kon Park, Taous Khan, Jae Yong Jung
    Abstract:

    Abstract Gluconacetobacter hansenii PJK, a cellulose producing bacterium recently isolated from the rotten apples, produced fair amounts of the water-soluble polysaccharides (WSPS). WSPS were studied for their monosaccharides composition after Acid hydrolysis, which revealed that the hydrolysates consist only of one sugar, Glucuronic Acid. The structure of the WSPS was investigated using various spectroscopic techniques including FT-IR, MALDI-TOF MS and 1 H, and 13 C NMR. These studies revealed that the product is a mixture of oligomers with the α-Glucuronic Acid as building blocks. The possible structure of the major oligosaccharide in the mixture has been deduced.

Ugur Hodoglugil - One of the best experts on this subject based on the ideXlab platform.

  • Glucuronic Acid epimerase is associated with plasma triglyceride and high density lipoprotein cholesterol levels in turks
    Annals of Human Genetics, 2011
    Co-Authors: Ugur Hodoglugil, David Williamson, Lindsay A Farrer, Robert W Mahley
    Abstract:

    We narrowed chromosome 15q21-23 linkage to plasma high-density lipoprotein cholesterol (HDL-C) levels in Turkish families by fine mapping, then focused on Glucuronic Acid epimerase (GLCE), a heparan sulfate proteoglycan (HSPG) biosynthesis enzyme. HSPGs participate in lipid metabolism along with apolipoprotein (apo) E. Of 31 SNPs in the GLCE locus, nine analyzed by haplotype were associated with HDL-C and triglyceride levels (permuted p = 0.006 and 0.013, respectively) in families. Of five tagging GLCE SNPs in two cohorts of unrelated subjects, three (rs16952868, rs11631403, and rs3865014) were associated with triglyceride and HDL-C levels in males (nonpermuted p < 0.05). The association was stronger in APOE 2/3 subjects (apoE2 has reduced binding to HSPGs) and reached multiple-testing significance (p < 0.05) in both males and females (n= 2612). Similar results were obtained in the second cohort (n= 1164). Interestingly, at the GLCE locus, bounded by recombination hotspots, Turks had a minor allele frequency of SNPs resembling Chinese more than European ancestry; adjoining regions resembled the European pattern. Studies of glce(+/-) apoe(-/-) mice fed a chow or high-fat diet supported a role for GLCE in lipid metabolism. Thus, SNPs in GLCE are associated with triglyceride and HDL-C levels in Turks, and mouse studies support a role for glce in lipid metabolism.

  • Glucuronic Acid epimerase is associated with plasma triglyceride and high density lipoprotein cholesterol levels in turks
    Annals of Human Genetics, 2011
    Co-Authors: Ugur Hodoglugil, David Williamson, Lindsay A Farrer, Yi Yu, Robert W Mahley
    Abstract:

    We narrowed chromosome 15q21-23 linkage to plasma high density lipoprotein cholesterol (HDL-C) levels in atherogenic dyslipidemic Turkish families by fine mapping, then focused on Glucuronic Acid epimerase (GLCE), a heparan sulfate proteoglycan (HSPG) biosynthesis enzyme. HSPGs participate in lipid metabolism along with apolipoprotein (apo) E. Of 31 SNPs in the GLCE locus, nine analyzed by haplotype were associated with plasma HDL-C and triglyceride levels (permuted p = 0.006 and 0.013, respectively) in families. Of five tagging GLCE SNPs in two cohorts of unrelated subjects, three (rs16952868, rs11631403, rs3865014) were associated with triglyceride and HDL-C levels in males (non-permuted p < 0.05). The association was stronger in APOE 2/3 subjects (apoE2 has reduced binding to HSPGs) and reached multiple-testing significance (p < 0.05) in both males and females (n = 2612). Similar results were obtained in the second cohort (n = 1164). Interestingly, at the GLCE locus, bounded by recombination hotspots, Turks had a minor allele frequency of SNPs resembling Chinese more than European ancestry; adjoining regions on chromosome 15 resembled the European pattern. Studies of glce+/–apoe–/– mice fed a chow or high-fat diet supported a role for GLCE in lipid metabolism. Thus, SNPs in GLCE are associated with triglyceride and HDL-C levels in Turks, and mouse studies support a role for glce in lipid metabolism.

Taous Khan - One of the best experts on this subject based on the ideXlab platform.

  • effects of Glucuronic Acid oligomers on the production structure and properties of bacterial cellulose
    Carbohydrate Polymers, 2013
    Co-Authors: Mazhar Ulislam, Taous Khan, Joong Kon Park
    Abstract:

    The addition of certain supplementary carbon sources to the culture media can influence the production, structural features and mechanical properties of bacterial cellulose (BC). In this study, different concentrations (0, 1, 2 and 4%) of a by-product, single sugar α-linked Glucuronic Acid-based oligosaccharide (SSGO), were added to the culture media during the production of BC. Production with 1% (BC1), 2% (BC2) and 4% (BC3) SSGO led to increases in BC production of 10.45, 12.74 and 9.01 g/L, respectively, after 10 days of cultivation under static conditions, while it was only 7.4 g/L when no SSGO was added (BC0). The structures of BC0, BC1, BC2, and BC3 were confirmed by XRD and FT-IR analysis. FE-SEM micrographs showed increased fibril thickness and decreased pore size in the SSGO added samples. The tensile strength of the BC0 was 16.73 MPa, while it was 25.05 MPa for BC1. However, with further increases in the concentration of SSGO, the tensile strength decreased to 20.76 and 19.77 MPa for BC2 and BC3, respectively. The results of this study provide further insight into the additive role of SSGO and improvement of the physico-mechanical properties of BC.

  • bacterial cellulose production from a single sugar α linked Glucuronic Acid based oligosaccharide
    Process Biochemistry, 2011
    Co-Authors: Nasrullah Shah, Taous Khan, Mazhar Ulislam, Joong Kon Park
    Abstract:

    Abstract A single sugar α-linked Glucuronic Acid-based oligosaccharide (SSGO), which is a by-product produced during bacterial cellulose (BC) synthesis by Gluconacetobacter hansenii , was evaluated as an additive to synthetic medium during BC production. We assessed the ability of the SSGO to serve as an alternative carbon source to glucose for BC production. To improve the BC yield from glucose by G. hansenii PJK, SSGO was added to a chemically defined medium (CDM). In the presence of 1% SSGO, the amount of BC produced was 10.5 g/L after 10 days of cultivation, whereas in the absence of SSGO, only 7.4 g/L of BC was produced. These results indicate that SSGO can be used as an alternative carbon source for BC production. Moreover, the addition of SSGO to the CDM increased the diameter of the BC fibers, which improved its tensile strength and water release properties.

  • the structure and physical properties of Glucuronic Acid oligomers produced by a gluconacetobacter hansenii strain using the waste from beer fermentation broth
    Carbohydrate Polymers, 2008
    Co-Authors: Taous Khan, Joong Kon Park
    Abstract:

    The aim of this work was to study the chemical structure and physical properties of water-soluble oligosaccharides (WSOS) produced by Gluconacetobacter hansenii PJK using the waste from beer fermentation broth as a basal medium. The analysis of the hydrolyzed products and the spectroscopic studies of the native WSOS showed that it is a mixture of oligomers all having a single sugar α-linked Glucuronic Acid as building blocks with an O-acetyl and O-methyl group, in the terminal unit of the non-reducing end. The thermal studies displayed a progressive degradation of WSOS without a major weight loss throughout a range of temperatures. The melting point and pyrolysis temperature were found to be 130.16 and 275.25 °C, respectively. The optimum concentration of WSOS for a maximum emulsifying ability was found to be 0.10% (w/v). The resulting emulsions, however, did not demonstrate a noteworthy stability.

  • structural studies of the Glucuronic Acid oligomers produced by gluconacetobacter hansenii strain
    Carbohydrate Polymers, 2006
    Co-Authors: Joong Kon Park, Taous Khan, Jae Yong Jung
    Abstract:

    Abstract Gluconacetobacter hansenii PJK, a cellulose producing bacterium recently isolated from the rotten apples, produced fair amounts of the water-soluble polysaccharides (WSPS). WSPS were studied for their monosaccharides composition after Acid hydrolysis, which revealed that the hydrolysates consist only of one sugar, Glucuronic Acid. The structure of the WSPS was investigated using various spectroscopic techniques including FT-IR, MALDI-TOF MS and 1 H, and 13 C NMR. These studies revealed that the product is a mixture of oligomers with the α-Glucuronic Acid as building blocks. The possible structure of the major oligosaccharide in the mixture has been deduced.

David Williamson - One of the best experts on this subject based on the ideXlab platform.

  • Glucuronic Acid epimerase is associated with plasma triglyceride and high density lipoprotein cholesterol levels in turks
    Annals of Human Genetics, 2011
    Co-Authors: Ugur Hodoglugil, David Williamson, Lindsay A Farrer, Robert W Mahley
    Abstract:

    We narrowed chromosome 15q21-23 linkage to plasma high-density lipoprotein cholesterol (HDL-C) levels in Turkish families by fine mapping, then focused on Glucuronic Acid epimerase (GLCE), a heparan sulfate proteoglycan (HSPG) biosynthesis enzyme. HSPGs participate in lipid metabolism along with apolipoprotein (apo) E. Of 31 SNPs in the GLCE locus, nine analyzed by haplotype were associated with HDL-C and triglyceride levels (permuted p = 0.006 and 0.013, respectively) in families. Of five tagging GLCE SNPs in two cohorts of unrelated subjects, three (rs16952868, rs11631403, and rs3865014) were associated with triglyceride and HDL-C levels in males (nonpermuted p < 0.05). The association was stronger in APOE 2/3 subjects (apoE2 has reduced binding to HSPGs) and reached multiple-testing significance (p < 0.05) in both males and females (n= 2612). Similar results were obtained in the second cohort (n= 1164). Interestingly, at the GLCE locus, bounded by recombination hotspots, Turks had a minor allele frequency of SNPs resembling Chinese more than European ancestry; adjoining regions resembled the European pattern. Studies of glce(+/-) apoe(-/-) mice fed a chow or high-fat diet supported a role for GLCE in lipid metabolism. Thus, SNPs in GLCE are associated with triglyceride and HDL-C levels in Turks, and mouse studies support a role for glce in lipid metabolism.

  • Glucuronic Acid epimerase is associated with plasma triglyceride and high density lipoprotein cholesterol levels in turks
    Annals of Human Genetics, 2011
    Co-Authors: Ugur Hodoglugil, David Williamson, Lindsay A Farrer, Yi Yu, Robert W Mahley
    Abstract:

    We narrowed chromosome 15q21-23 linkage to plasma high density lipoprotein cholesterol (HDL-C) levels in atherogenic dyslipidemic Turkish families by fine mapping, then focused on Glucuronic Acid epimerase (GLCE), a heparan sulfate proteoglycan (HSPG) biosynthesis enzyme. HSPGs participate in lipid metabolism along with apolipoprotein (apo) E. Of 31 SNPs in the GLCE locus, nine analyzed by haplotype were associated with plasma HDL-C and triglyceride levels (permuted p = 0.006 and 0.013, respectively) in families. Of five tagging GLCE SNPs in two cohorts of unrelated subjects, three (rs16952868, rs11631403, rs3865014) were associated with triglyceride and HDL-C levels in males (non-permuted p < 0.05). The association was stronger in APOE 2/3 subjects (apoE2 has reduced binding to HSPGs) and reached multiple-testing significance (p < 0.05) in both males and females (n = 2612). Similar results were obtained in the second cohort (n = 1164). Interestingly, at the GLCE locus, bounded by recombination hotspots, Turks had a minor allele frequency of SNPs resembling Chinese more than European ancestry; adjoining regions on chromosome 15 resembled the European pattern. Studies of glce+/–apoe–/– mice fed a chow or high-fat diet supported a role for GLCE in lipid metabolism. Thus, SNPs in GLCE are associated with triglyceride and HDL-C levels in Turks, and mouse studies support a role for glce in lipid metabolism.