The Experts below are selected from a list of 228 Experts worldwide ranked by ideXlab platform
Jun Yang - One of the best experts on this subject based on the ideXlab platform.
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Oxytocin in hypothalamic supraoptic nucleus is transferred to the caudate nucleus to influence pain modulation
Neuropeptides, 2016Co-Authors: Yang-juan Pan, Jun Yang, Da-xin Wang, Xiao Naimin, Chang-hong Wang, Bao-cheng LinAbstract:Oxytocin (OXT), which is synthesized and secreted in the hypothalamic supraoptic nucleus (SON), is the most important bioactive substance in SON regulating pain process. Our previous study has pointed that OXT in the caudate nucleus (CdN) plays a role in pain modulation. The communication was designed to investigate the source of OXT in the rat CdN during pain process using the methods of push-pull perfusion and radioimmunoassay. The results showed that (1) pain stimulation increased the OXT concentration in the CdN perfusion liquid; (2) SON cauterization inhibited the increase of OXT concentration in CdN perfusion liquid induced by the pain stimulation, which role in both sides of SON cauterization was stronger than that in one side of SON cauterization; and (3) SON microinjection of l-Glutamate Sodium, which excited the SON neurons, increased OXT concentration in the CdN perfusion liquid. The data suggested that OXT in the CdN was influenced by SON during pain process, i.e., OXT in the SON might be transferred to the CdN to influence pain modulation.
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Oxytocin, but not arginine vasopressin is involving in the antinociceptive role of hypothalamic supraoptic nucleus
Peptides, 2011Co-Authors: Jun Yang, Jin-ying Liang, Xiao-yi Zhang, Pei-yong Qiu, Yan-juan Pan, Jing Zhang, Fang Hao, Da-xin Wang, Fu-lin YanAbstract:Abstract Our pervious study has demonstrated that the hypothalamic supraoptic nucleus (SON) plays a role in pain modulation. Oxytocin (OXT) and arginine vasopressin (AVP) are the important hormones synthesized and secreted by the SON. The experiment was designed to investigate which hormone was relating with the antinociceptive role of the SON in the rat. The results showed that (1) microinjection of l -Glutamate Sodium into the SON increased OXT and AVP concentrations in the SON perfusion liquid, (2) pain stimulation induces OXT, but not AVP release in the SON, and (3) intraventricular injection (pre-treatment) with OXT antiserum could inhibit the pain threshold increase induced by SON injection of l -Glutamate Sodium, but administration of AVP antiserum did not influence the antinociceptive role of SON stimulation. The data suggested that the antinociceptive role of the SON relates to OXT rather than AVP.
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Arginine vasopressin in hypothalamic paraventricular nucleus is transferred to the caudate nucleus to participate in pain modulation.
Peptides, 2010Co-Authors: Jun Yang, Xiao-yi Zhang, Yan-juan Pan, Jing Zhang, Fang Hao, Da-xin Wang, WangAbstract:Abstract Arginine vasopressin (AVP), which is synthesized and secreted in the hypothalamic paraventricular nucleus (PVN), is the most important bioactive substance in the pain modulation. Our pervious study had shown that AVP plays an important role in pain modulation in caudate nucleus (CdN). The experiment was designed to investigate the source of AVP in CdN by the nucleus push–pull perfusion and radioimmunoassay. The results showed that: (1) pain stimulation increased the AVP concentration in the CdN perfusion liquid, (2) PVN decreased the effect of pain stimulation which was stronger in both sides than in one side of PVN cauterization; and (3) L-Glutamate Sodium would excited the PVN neurons by the PVN microinjection that could increase the AVP concentration in the CdN perfusion liquid. The data suggested that AVP in the CdN might come from the PVN in the pain process, i.e., AVP in the PVN might be transferred to the CdN to participate in the pain modulation.
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Norepinephrine plays an important role in antinociceptive modulation of hypothalamic paraventricular nucleus in the rat.
The International journal of neuroscience, 2010Co-Authors: Xijian Zhou, Jun Yang, Fang Hao, Da-xin Wang, Fu-lin Yan, Xiao-qiang Fan, Xi-qing YanAbstract:Our previous study has proven that hypothalamic paraventricular nucleus (PVN) plays a role in antinociception. The effects of studied classical neurotransmitter on PVN antinociceptive modulation were investigated in the rat. The results showed: (1) Pain stimulation increased norepinephrine (NE), but not epinephrine, dopamine (DA), 3,4-dihydroxyphenylacetic acid (DA metabolic product), homovanilic acid (DA metabolic product), serotonin (5-HT), 5-hydroxyindoleacetic acid (5-HT metabolic product), acetycholine (Ach), choline (Ach metabolic product), gamma-aminobutyric acid (GABA), and L-Glutamate acid concentrations in the PVN perfusion liquid; (2) PVN stimulation with L-Glutamate Sodium, which excited local neurons only, did not influence the concentrations of the studied classical neurotransmitter and metabolic product in the PVN perfusion liquid; (3) Microinjection of NE, epinephrine, or L-Glutamate Sodium into the PVN elevated pain threshold, and local administration of GABA decreased pain threshold in a dose-dependent manner, but PVN administration of Ach, DA, or 5-HT did not change pain threshold; (4) Microinjection of phentolamine (alpha-receptor antagonist) or MK801 [NMDA-receptor antagonist] into the PVN reduced pain threshold, and local administration of bicuculline (GABA-receptor antagonist) raised pain threshold, but PVN administration of propranolol (beta-receptor antagonist), atropine (Muscarinic cholinergic receptor antagonist), 6-OH gallamine (Nicotinic cholinergic receptor antagonist), fluperidol (DA-receptor antagonist), or cyproheptadine (5-HT-receptor antagonist) did not alter pain threshold. The data suggested that endogenous NE, not epinephrine, 5-HT, Ach, GABA, and L-Glutamate acid played an important role in the PVN antinociceptive modulation.
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Norepinephrine regulates arginine vasopressin secretion in hypothalamic paraventricular nucleus relating with pain modulation.
Neuropeptides, 2009Co-Authors: Jun Yang, Wen-yan Liu, Huifeng Yuan, Xiao-xia Zhang, Feng Jianpeng, Da-wei Yang, WangAbstract:Abstract Our previous study has pointed that arginine vasopressin (AVP) and norepinephrine (NA) are two most important bioactive substances that play a role in hypothalamic paraventricular nucleus (PVN) regulating pain process. The communication was designed to investigate the interaction between AVP and NA in the rat PVN during the pain process. We used the potassium iontophoresis inducing tail-flick to test the pain threshold, PVN push–pull perfusion to collect the samples, high performance chromatography (HPLC) to determine the NA concentration and radioimmunoassay (RIA) to measure the AVP concentration. The results showed that (1) pain stimulation increased both NA and AVP concentrations in the PVN perfusion liquid; (2) PVN administration of l-Glutamate Sodium increased AVP, not NA concentration in the PVN perfusion liquid; (3) AVP or d(CH 2 ) 5 Tyr(Et)DAVP (AVP-receptor antagonist) neither changed pain threshold, nor influenced NA concentration in the PVN perfusion liquid; (4) Microinjection of NA into PVN could increase pain threshold in a dose-dependent manner, while PVN administration with phentolamine (α-receptor antagonist), not propranolol (β-receptor antagonist) decreased pain threshold; (5) Administration of NA increased AVP concentration, while phentolamine, not propranolol decreased AVP concentration in the PVN perfusion liquid. These data suggested that it is through α-receptor rather than β-receptor, NA induced PVN secretion of AVP that was delivered to the related brain regions to participate in pain modulation.
Bao-cheng Lin - One of the best experts on this subject based on the ideXlab platform.
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Oxytocin in hypothalamic supraoptic nucleus is transferred to the caudate nucleus to influence pain modulation
Neuropeptides, 2016Co-Authors: Yang-juan Pan, Jun Yang, Da-xin Wang, Xiao Naimin, Chang-hong Wang, Bao-cheng LinAbstract:Oxytocin (OXT), which is synthesized and secreted in the hypothalamic supraoptic nucleus (SON), is the most important bioactive substance in SON regulating pain process. Our previous study has pointed that OXT in the caudate nucleus (CdN) plays a role in pain modulation. The communication was designed to investigate the source of OXT in the rat CdN during pain process using the methods of push-pull perfusion and radioimmunoassay. The results showed that (1) pain stimulation increased the OXT concentration in the CdN perfusion liquid; (2) SON cauterization inhibited the increase of OXT concentration in CdN perfusion liquid induced by the pain stimulation, which role in both sides of SON cauterization was stronger than that in one side of SON cauterization; and (3) SON microinjection of l-Glutamate Sodium, which excited the SON neurons, increased OXT concentration in the CdN perfusion liquid. The data suggested that OXT in the CdN was influenced by SON during pain process, i.e., OXT in the SON might be transferred to the CdN to influence pain modulation.
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Arginine vasopressin in hypothalamic paraventricular nucleus is transferred to the nucleus raphe magnus to participate in pain modulation.
Peptides, 2009Co-Authors: Jun Yang, Wen-yan Liu, Huifeng Yuan, Wang, Cai Song, Daiwei Yang, Bao-cheng LinAbstract:Hypothalamic paraventricular nucleus (PVN) is one of the main sources of arginine vasopressin (AVP) synthesis and secretion. AVP is the most important bioactive substance in PVN regulating pain process. Our previous study has pointed that pain stimulation induced AVP increase in the nucleus raphe magnus (NRM), which plays a role in pain modulation. The present study was designed to investigate the source of AVP in the rat NRM during pain process using the methods of nucleus push-pull perfusion and radioimmunoassay. The results showed that pain stimulation increased the AVP concentration in the NRM perfusion liquid, PVN cauterization inhibited the role that pain stimulation induced the increase of AVP concentration in the NRM perfusion liquid, and PVN microinjection of L-Glutamate Sodium, which excited the PVN neurons, could increase the AVP concentration in the NRM perfusion liquid. The data suggested that AVP in the PVN might be transferred to the NRM to participate in pain modulation.
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Endogenous opiate peptides in the spinal cord are involved in the analgesia of hypothalamic paraventricular nucleus in the rat.
Peptides, 2009Co-Authors: Jun Yang, Yu Yang, Wen-yan Liu, Wang, Chenghai Wang, Jiegen Chu, Bao-cheng LinAbstract:Abstract Many studies have shown that hypothalamic paraventricular nucleus (PVN) plays a role in pain process, and endogenous opiate peptide system in the spinal cord is involved in nociception. This communication was designed to study the relationship between PVN and endogenous opiate system in the spinal cord in the rat. The results showed that in both the thoracic and the lumber spinal cord, microinjection of 100 ng l -Glutamate Sodium into PVN could increase leucine-enkephalin (L-Ek), β-endorphin (β-Ep), dynorphinA 1–13 (DynA 1–13 ) concentrations and PVN cauterization decreased L-Ek and β-Ep concentrations. Pretreatment of the spinal cord with 5 μg naloxone, an opiate receptor antagonist could partly reverse the analgesia induced by microinjection of 100 ng l -Glutamate Sodium into PVN. The data suggested that PVN analgesia might be involved in the endogenous opiate peptide system in the spinal cord independently.
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Investigating the Role of Hypothalamic Paraventricular Nucleus in Nociception of the Rat
The International journal of neuroscience, 2008Co-Authors: Jun Yang, Yu Yang, Jian-min Chen, Wen-yan Liu, Cao-you Song, Bao-cheng LinAbstract:The role of hypothalamic paraventricular nucleus (PVN) in nociception was investigated in the rat. Electrical stimulation of the PVN increased pain threshold, and microinjection of L-Glutamate Sodium into the PVN also elevated pain threshold in a dose-dependent manner, whereas cauterization of the PVN decreased pain threshold. Stimulation or cauterization of the area located within 1.0 mm of the outer perimeter of the PVN did not change pain threshold. Pituitary removal could not influence the effect of L-Glutamate Sodium microinjection into PVN-induced pain threshold increase. The data suggest that PVN plays a role in antinociception through the central nervous system rather than peripheral organs.
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Arginine vasopressin is an important regulator in antinociceptive modulation of hypothalamic paraventricular nucleus in the rat.
Neuropeptides, 2007Co-Authors: Jun Yang, Yu Yang, Jian-min Chen, Wen-yan Liu, Chenghai Wang, Bao-cheng LinAbstract:Abstract Our pervious study has proven that hypothalamic paraventricular nucleus (PVN) stimulation increases pain threshold and PVN cauterization decreases pain threshold. The studied neuropeptides in PVN were investigated to involve to pain modulation in the rat. The results showed that (1) intraventricular injection ( icv ) of anti-arginine vasopressin (AVP) serum completely reversed pain threshold increase induced by l -Glutamate Sodium (Glu) injection into the PVN, and local administration ( icv ) of anti-leucine-enkephalin (L-Ek) serum or anti-β-endorphin (β-Ep) serum partly attenuated pain threshold increase induced by Glu injection into the PVN, but pre-treatment of anti-oxytocin (OXT), dynorphinA 1-13 (DynA 1-13 ), cholecystokinin-like peptide (CCK), neurotensin (NT), corticotrophin-releasing hormone (CRH), adrenocorticotrophin (ACTH), somatostatin (SST), prolactin-releasing hormone (PRH), angiotensinII (AngII), vasoactive intestinal polypeptide (VIP), melanotropin-releasing hormone (MRH), thyrotropin-releasing hormone (TRH), substance P (SP) or growth hormone-releasing hormone (GHRH) serum ( icv ) did not influence the analgesic effect of PVN administration with Glu; (2) PVN stimulation with Glu elevated the concentrations of AVP, OXT, CCK, NT, CRH, SST, PRH and DynA 1-13 in PVN perfusion liquid, and could not change the concentrations of L-Ek, β-Ep, AngII, ACTH, VIP, MRH, TRH, SP and GHRH in PVN perfusion liquid; (3) Pain stimulation increased the concentrations of AVP, L-Ek, β-Ep, DynA 1-13 , CRH and ACTH in PVN perfusion liquid, and did not alter the concentrations of OXT, CCK, NT, SST, PRH, AngII, VIP, MRH, TRH, SP and GHRH in PVN perfusion liquid. The data suggested that AVP played a more important role than the other studied peptides (OXT, L-Ek, β-Ep, DynA 1-13 , CCK, NT, CRH, ACTH, SST, PRH, AngII, VIP, MRH, TRH, SP and GHRH) in PVN antinociceptive progress.
Wen-yan Liu - One of the best experts on this subject based on the ideXlab platform.
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Norepinephrine regulates arginine vasopressin secretion in hypothalamic paraventricular nucleus relating with pain modulation.
Neuropeptides, 2009Co-Authors: Jun Yang, Wen-yan Liu, Huifeng Yuan, Xiao-xia Zhang, Feng Jianpeng, Da-wei Yang, WangAbstract:Abstract Our previous study has pointed that arginine vasopressin (AVP) and norepinephrine (NA) are two most important bioactive substances that play a role in hypothalamic paraventricular nucleus (PVN) regulating pain process. The communication was designed to investigate the interaction between AVP and NA in the rat PVN during the pain process. We used the potassium iontophoresis inducing tail-flick to test the pain threshold, PVN push–pull perfusion to collect the samples, high performance chromatography (HPLC) to determine the NA concentration and radioimmunoassay (RIA) to measure the AVP concentration. The results showed that (1) pain stimulation increased both NA and AVP concentrations in the PVN perfusion liquid; (2) PVN administration of l-Glutamate Sodium increased AVP, not NA concentration in the PVN perfusion liquid; (3) AVP or d(CH 2 ) 5 Tyr(Et)DAVP (AVP-receptor antagonist) neither changed pain threshold, nor influenced NA concentration in the PVN perfusion liquid; (4) Microinjection of NA into PVN could increase pain threshold in a dose-dependent manner, while PVN administration with phentolamine (α-receptor antagonist), not propranolol (β-receptor antagonist) decreased pain threshold; (5) Administration of NA increased AVP concentration, while phentolamine, not propranolol decreased AVP concentration in the PVN perfusion liquid. These data suggested that it is through α-receptor rather than β-receptor, NA induced PVN secretion of AVP that was delivered to the related brain regions to participate in pain modulation.
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Arginine vasopressin in hypothalamic paraventricular nucleus is transferred to the nucleus raphe magnus to participate in pain modulation.
Peptides, 2009Co-Authors: Jun Yang, Wen-yan Liu, Huifeng Yuan, Wang, Cai Song, Daiwei Yang, Bao-cheng LinAbstract:Hypothalamic paraventricular nucleus (PVN) is one of the main sources of arginine vasopressin (AVP) synthesis and secretion. AVP is the most important bioactive substance in PVN regulating pain process. Our previous study has pointed that pain stimulation induced AVP increase in the nucleus raphe magnus (NRM), which plays a role in pain modulation. The present study was designed to investigate the source of AVP in the rat NRM during pain process using the methods of nucleus push-pull perfusion and radioimmunoassay. The results showed that pain stimulation increased the AVP concentration in the NRM perfusion liquid, PVN cauterization inhibited the role that pain stimulation induced the increase of AVP concentration in the NRM perfusion liquid, and PVN microinjection of L-Glutamate Sodium, which excited the PVN neurons, could increase the AVP concentration in the NRM perfusion liquid. The data suggested that AVP in the PVN might be transferred to the NRM to participate in pain modulation.
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Endogenous opiate peptides in the spinal cord are involved in the analgesia of hypothalamic paraventricular nucleus in the rat.
Peptides, 2009Co-Authors: Jun Yang, Yu Yang, Wen-yan Liu, Wang, Chenghai Wang, Jiegen Chu, Bao-cheng LinAbstract:Abstract Many studies have shown that hypothalamic paraventricular nucleus (PVN) plays a role in pain process, and endogenous opiate peptide system in the spinal cord is involved in nociception. This communication was designed to study the relationship between PVN and endogenous opiate system in the spinal cord in the rat. The results showed that in both the thoracic and the lumber spinal cord, microinjection of 100 ng l -Glutamate Sodium into PVN could increase leucine-enkephalin (L-Ek), β-endorphin (β-Ep), dynorphinA 1–13 (DynA 1–13 ) concentrations and PVN cauterization decreased L-Ek and β-Ep concentrations. Pretreatment of the spinal cord with 5 μg naloxone, an opiate receptor antagonist could partly reverse the analgesia induced by microinjection of 100 ng l -Glutamate Sodium into PVN. The data suggested that PVN analgesia might be involved in the endogenous opiate peptide system in the spinal cord independently.
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Investigating the Role of Hypothalamic Paraventricular Nucleus in Nociception of the Rat
The International journal of neuroscience, 2008Co-Authors: Jun Yang, Yu Yang, Jian-min Chen, Wen-yan Liu, Cao-you Song, Bao-cheng LinAbstract:The role of hypothalamic paraventricular nucleus (PVN) in nociception was investigated in the rat. Electrical stimulation of the PVN increased pain threshold, and microinjection of L-Glutamate Sodium into the PVN also elevated pain threshold in a dose-dependent manner, whereas cauterization of the PVN decreased pain threshold. Stimulation or cauterization of the area located within 1.0 mm of the outer perimeter of the PVN did not change pain threshold. Pituitary removal could not influence the effect of L-Glutamate Sodium microinjection into PVN-induced pain threshold increase. The data suggest that PVN plays a role in antinociception through the central nervous system rather than peripheral organs.
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Effect of hypothalamic supraoptic nucleus on acupuncture analgesia in the rat.
Brain research bulletin, 2007Co-Authors: Jun Yang, Yu Yang, Jian-min Chen, Wen-yan Liu, Bao-chen LinAbstract:Hypothalamic supraoptic nucleus (SON) has been demonstrated to involve in pain modulation. Acupuncture analgesia is a very useful clinical skill for pain relief, which has over 2500-year history in China. The present study investigated the effect of SON on acupuncture analgesia in the rat. Electrical stimulation of the SON or microinjection of a small dose L-Glutamate Sodium into the SON enhanced acupuncture analgesia in a dose-dependent manner, while cauterization of the SON weakened acupuncture analgesia. Pituitary removal did not influence the effect of L-Glutamate Sodium that enhanced acupuncture analgesia in the SON. The data suggested that the neurons and not the nerve fibers in the SON played an important role in acupuncture analgesia, which effect might be through the central nervous system rather than the hypothalamo-neurohypophyseal system.
G. Prezioso - One of the best experts on this subject based on the ideXlab platform.
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The Kinetic Mechanism of the Glutamate–Aspartate Carrier in Rat Intestinal Brush-Border Membrane Vesicles: The Role of Potassium
Journal of Bioenergetics and Biomembranes, 2002Co-Authors: V. Scalera, M. G. Mola, G. PreziosoAbstract:The Sodium dependent transport system for L-Glutamate and L-aspartate localized in the apical part of rat enterocytes has previously been kinetically characterized (Prezioso, G., and Scalera, V. (1996). Biochim. Biophys. Acta 1279 , 144–148). In this paper the mechanism by which the potassium cation specifically activates the L-Glutamate–Sodium cotransport process is investigated. Potassium has been found to act as an activator when it is present inside the membrane vesicles, while its presence outside is ineffective, and the effect is saturable. The kinetic parameters with respect to Sodium and Glutamate have been compared in the presence and in the absence of the activator. The results indicate that the ordered Sodium–Sodium Glutamate mechanism is not altered by potassium, and that the activation is probably exerted on both the rate determining steps of the transport process. It is proposed that (1) a specific binding site for potassium is present on the inside hydrophilic part of the membrane carrier, (2) the binding of the effector accelerates the intramembrane rearrangement steps of both the diSodium Glutamate–carrier complex and the free carrier, (3) the affinity of the carrier is lowered with respect to Sodium whereas it is increased for Glutamate, and (4) K^+ antiport is not performed by this carrier.
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The kinetic mechanism of the Glutamate-aspartate carrier in rat intestinal brush-border membrane vesicles: the role of potassium.
Journal of bioenergetics and biomembranes, 2002Co-Authors: V. Scalera, M. G. Mola, G. PreziosoAbstract:The Sodium dependent transport system for L-Glutamate and L-aspartate localized in the apical part of rat enterocytes has previously been kinetically characterized (Prezioso, G., and Scalera, V. (1996). Biochim. Biophys. Acta1279, 144–148). In this paper the mechanism by which the potassium cation specifically activates the L-Glutamate–Sodium cotransport process is investigated. Potassium has been found to act as an activator when it is present inside the membrane vesicles, while its presence outside is ineffective, and the effect is saturable. The kinetic parameters with respect to Sodium and Glutamate have been compared in the presence and in the absence of the activator. The results indicate that the ordered Sodium–Sodium Glutamate mechanism is not altered by potassium, and that the activation is probably exerted on both the rate determining steps of the transport process. It is proposed that (1) a specific binding site for potassium is present on the inside hydrophilic part of the membrane carrier, (2) the binding of the effector accelerates the intramembrane rearrangement steps of both the diSodium Glutamate–carrier complex and the free carrier, (3) the affinity of the carrier is lowered with respect to Sodium whereas it is increased for Glutamate, and (4) K+ antiport is not performed by this carrier.
Mindaugas Malakauskas - One of the best experts on this subject based on the ideXlab platform.
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The antimicrobial effect of spice-based marinades against Campylobacter jejuni on contaminated fresh broiler wings.
Journal of food science, 2015Co-Authors: Gintarė Zakarienė, Anita Rokaitytė, Sigita Ramonaitė, Aleksandr Novoslavskij, Kristina Mulkytė, Gintarė Zaborskienė, Mindaugas MalakauskasAbstract:The antimicrobial effect of spice-based marinades against Campylobacter jejuni on inoculated fresh broiler wings was investigated. Experiments were carried out with 1 strain of C. jejuni and 6 marinades. Four experimental marinades were composed for the study and contained spices (thyme, rosemary, basil, marjoram, and so on) and different combination of bioactive compounds. Two marinades were commercial and contained spices (black pepper, sweet red pepper, and so on) and chemical additives (monoSodium Glutamate, Sodium diacetate, calcium lactate), 1 commercial marinade was also enriched with bioactive compounds (linalool, cinnamaldehyde, lactic acid). Total aerobic bacterial count was examined to estimate the possible effect of tested marinades on the shelf-life of marinated broiler wings. Study revealed that thyme-based marinade was the most effective against C. jejuni on broiler wings and reduced the numbers of campylobacters by 1.04 log colony forming unit (CFU)/g (P ≤ 0.05) during storage for 168 h at 4 °C temperature. Moreover, it was more effective against C. jejuni than commercial marinade with 0.47 log CFU/g (P ≤ 0.05) reduction effect. Both experimental and commercial marinades had very similar effect on the total aerobic bacterial count. Although experimental and commercial marinades had different effect on pH of broiler wings, this parameter did not show a major impact on the antimicrobial effect of tested marinades (P ≥ 0.05). Our study shows that experimental natural thyme-based marinade can reduce numbers of C. jejuni more effectively than tested commercial marinades. Practical Application Experimental spice-based marinades could be used for marinating broiler meat to ensure the enhanced safety of contaminated broiler meat by reducing Campylobacter jejuni counts. Thyme-based and rosemary-based marinades may reduce public health risk to acquire campylobacteriosis through miss-handling and consumption of marinated broiler meat and satisfy consumers demand for more natural broiler meat products.
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The Antimicrobial Effect of Spice-Based Marinades against Campylobacter jejuni on Contaminated Fresh Broiler Wings GintarZakarienAnita RokaitytSigita RamonaitAleksandr Novoslavskij, Kristina MulkytGintarZaborskien˙
2015Co-Authors: Mindaugas MalakauskasAbstract:The antimicrobial effect of spice-based marinades against Campylobacter jejuni on inoculated fresh broiler wings was investigated. Experiments were carried out with 1 strain of C. jejuni and 6 marinades. Four experimental marinades were composed for the study and contained spices (thyme, rosemary, basil, marjoram, and so on) and different combination of bioactive compounds. Two marinades were commercial and contained spices (black pepper, sweet red pepper, and so on) and chemical additives (monoSodium Glutamate, Sodium diacetate, calcium lactate), 1 commercial marinade was also enriched with bioactive compounds (linalool, cinnamaldehyde, lactic acid). Total aerobic bacterial count was examined to estimate the possible effect of tested marinades on the shelf-life of marinated broiler wings. Study revealed that thyme-based marinade was the most effective against C. jejuni on broiler wings and reduced the numbers of campylobacters by 1.04 log colony forming unit (CFU)/g (P 0.05) during storage for 168 h at 4 ° Ct emperature. Moreover, it was more effective against C. jejuni than commercial marinade with 0.47 log CFU/g (P 0.05) reduction effect. Both experimental and commercial marinades had very similar effect on the total aerobic bacterial count. Although experimental and commercial marinades had different effect on pH of broiler wings, this parameter did not show a major impact on the antimicrobial effect of tested marinades (P 0.05). Our study shows that experimental natural thyme-based marinade can reduce numbers of C. jejuni more effectively than tested commercial marinades.