The Experts below are selected from a list of 27 Experts worldwide ranked by ideXlab platform

Carmen Marco - One of the best experts on this subject based on the ideXlab platform.

  • Incorporation of exogenous precursors into neutral lipids and phospholipids in rat hepatocytes: effect of ethanol in vitro
    Biochemical pharmacology, 1998
    Co-Authors: María P. Carrasco, Josefa L. Segovia, Carmen Marco
    Abstract:

    We studied the incorporation of different radioactively labeled exogenous substrates into the lipids of rat hepatocytes previously incubated with ethanol. Glycerol, Oleate, and serine were all incorporated into neutral lipids to a significantly greater degree in the presence of ethanol, the increase in radioactivity in the triacylGlycerol fraction being quite substantial. A similar ethanol-induced increase was found in the incorporation of these substrates into the various phospholipids. This lipogenic activity did not occur when the metabolism of ethanol was blocked by 4-methylpyrazole, an inhibitor of hepatic ADH (alcohol:NAD+ oxidoreductase, EC 1.1.1.1) activity, thus demonstrating that one of the initial effects of ethanol on lipid biosynthesis was mediated by some products of its metabolism in the liver. The only alteration that persisted in the presence of 4-methylpyrazole was an inhibitory effect on the esterification of free cholesterol from Oleate, suggesting that ethanol specifically inhibits hepatic ACAT (acyl CoA:cholesterol O-acyltransferase, EC 2.3.1.26) activity.

  • SHORT COMMUNICATION Incorporation of Exogenous Precursors into Neutral Lipids and Phospholipids in Rat Hepatocytes: Effect of Ethanol In Vitro
    1998
    Co-Authors: Josefa L. Segovia, Carmen Marco
    Abstract:

    We studied the incorporation of different radioactively labeled exogenous substrates into the lipids of rat hepatocytes previously incubated with ethanol. Glycerol, Oleate, and serine were all incorporated into neutral lipids to a significantly greater degree in the presence of ethanol, the increase in radioactivity in the triacylGlycerol fraction being quite substantial. A similar ethanol-induced increase was found in the incorpora- tion of these substrates into the various phospholipids. This lipogenic activity did not occur when the metabolism of ethanol was blocked by 4-methylpyrazole, an inhibitor of hepatic ADH (alcohol:NAD 1 oxidoreductase, EC 1.1.1.1) activity, thus demonstrating that one of the initial effects of ethanol on lipid biosynthesis was mediated by some products of its metabolism in the liver. The only alteration that persisted in the presence of 4-methylpyrazole was an inhibitory effect on the esterification of free cholesterol from Oleate, suggesting that ethanol specifically inhibits hepatic ACAT (acyl CoA:cholesterol O-acyltransferase, EC 2.3.1.26) activity. BIOCHEM PHARMACOL 56;12:1639 -1644, 1998. © 1998 Elsevier Science Inc.

María P. Carrasco - One of the best experts on this subject based on the ideXlab platform.

  • Incorporation of exogenous precursors into neutral lipids and phospholipids in rat hepatocytes: effect of ethanol in vitro
    Biochemical pharmacology, 1998
    Co-Authors: María P. Carrasco, Josefa L. Segovia, Carmen Marco
    Abstract:

    We studied the incorporation of different radioactively labeled exogenous substrates into the lipids of rat hepatocytes previously incubated with ethanol. Glycerol, Oleate, and serine were all incorporated into neutral lipids to a significantly greater degree in the presence of ethanol, the increase in radioactivity in the triacylGlycerol fraction being quite substantial. A similar ethanol-induced increase was found in the incorporation of these substrates into the various phospholipids. This lipogenic activity did not occur when the metabolism of ethanol was blocked by 4-methylpyrazole, an inhibitor of hepatic ADH (alcohol:NAD+ oxidoreductase, EC 1.1.1.1) activity, thus demonstrating that one of the initial effects of ethanol on lipid biosynthesis was mediated by some products of its metabolism in the liver. The only alteration that persisted in the presence of 4-methylpyrazole was an inhibitory effect on the esterification of free cholesterol from Oleate, suggesting that ethanol specifically inhibits hepatic ACAT (acyl CoA:cholesterol O-acyltransferase, EC 2.3.1.26) activity.

Josefa L. Segovia - One of the best experts on this subject based on the ideXlab platform.

  • Incorporation of exogenous precursors into neutral lipids and phospholipids in rat hepatocytes: effect of ethanol in vitro
    Biochemical pharmacology, 1998
    Co-Authors: María P. Carrasco, Josefa L. Segovia, Carmen Marco
    Abstract:

    We studied the incorporation of different radioactively labeled exogenous substrates into the lipids of rat hepatocytes previously incubated with ethanol. Glycerol, Oleate, and serine were all incorporated into neutral lipids to a significantly greater degree in the presence of ethanol, the increase in radioactivity in the triacylGlycerol fraction being quite substantial. A similar ethanol-induced increase was found in the incorporation of these substrates into the various phospholipids. This lipogenic activity did not occur when the metabolism of ethanol was blocked by 4-methylpyrazole, an inhibitor of hepatic ADH (alcohol:NAD+ oxidoreductase, EC 1.1.1.1) activity, thus demonstrating that one of the initial effects of ethanol on lipid biosynthesis was mediated by some products of its metabolism in the liver. The only alteration that persisted in the presence of 4-methylpyrazole was an inhibitory effect on the esterification of free cholesterol from Oleate, suggesting that ethanol specifically inhibits hepatic ACAT (acyl CoA:cholesterol O-acyltransferase, EC 2.3.1.26) activity.

  • SHORT COMMUNICATION Incorporation of Exogenous Precursors into Neutral Lipids and Phospholipids in Rat Hepatocytes: Effect of Ethanol In Vitro
    1998
    Co-Authors: Josefa L. Segovia, Carmen Marco
    Abstract:

    We studied the incorporation of different radioactively labeled exogenous substrates into the lipids of rat hepatocytes previously incubated with ethanol. Glycerol, Oleate, and serine were all incorporated into neutral lipids to a significantly greater degree in the presence of ethanol, the increase in radioactivity in the triacylGlycerol fraction being quite substantial. A similar ethanol-induced increase was found in the incorpora- tion of these substrates into the various phospholipids. This lipogenic activity did not occur when the metabolism of ethanol was blocked by 4-methylpyrazole, an inhibitor of hepatic ADH (alcohol:NAD 1 oxidoreductase, EC 1.1.1.1) activity, thus demonstrating that one of the initial effects of ethanol on lipid biosynthesis was mediated by some products of its metabolism in the liver. The only alteration that persisted in the presence of 4-methylpyrazole was an inhibitory effect on the esterification of free cholesterol from Oleate, suggesting that ethanol specifically inhibits hepatic ACAT (acyl CoA:cholesterol O-acyltransferase, EC 2.3.1.26) activity. BIOCHEM PHARMACOL 56;12:1639 -1644, 1998. © 1998 Elsevier Science Inc.

Yongsheng Chen - One of the best experts on this subject based on the ideXlab platform.

  • Characterization of a novel sn1,3 lipase from Ricinus communis L. suitable for production of oleic acid-palmitic acid-Glycerol Oleate.
    Scientific reports, 2021
    Co-Authors: Huajun Sun, Yongsheng Chen
    Abstract:

    The hydrolysis properties of lipase in castor was evaluated using two different substrate forms (tripalmitic glycerides and trioleic glycerides) to catalyze the reaction under different operational conditions. RcLipase was obtained from castor seeds and results show that RcLipase is a conservative serine lipase with a conserved catalytic center (SDH) and a conserved pentapeptide (GXSXG). This enzyme exhibited the greatest activity and tolerance to chloroform and toluene when it was expressed in Pichia pastoris GS115 at 40 ℃ and pH 8.0. Zn and Cu ions exerted obvious inhibitory effects on the enzyme, and displayed good hydrolytic activity for long-chain natural and synthetic lipids. HPLC analysis showed that this enzyme has 1,3 regioselectivity when Glycerol tripalmitate and oleic acid are used as substrates. The fatty acid composition in the reaction product was 21.3% oleic acid and 79.1% sn-2 palmitic acid.

Huajun Sun - One of the best experts on this subject based on the ideXlab platform.

  • Characterization of a novel sn1,3 lipase from Ricinus communis L. suitable for production of oleic acid-palmitic acid-Glycerol Oleate.
    Scientific reports, 2021
    Co-Authors: Huajun Sun, Yongsheng Chen
    Abstract:

    The hydrolysis properties of lipase in castor was evaluated using two different substrate forms (tripalmitic glycerides and trioleic glycerides) to catalyze the reaction under different operational conditions. RcLipase was obtained from castor seeds and results show that RcLipase is a conservative serine lipase with a conserved catalytic center (SDH) and a conserved pentapeptide (GXSXG). This enzyme exhibited the greatest activity and tolerance to chloroform and toluene when it was expressed in Pichia pastoris GS115 at 40 ℃ and pH 8.0. Zn and Cu ions exerted obvious inhibitory effects on the enzyme, and displayed good hydrolytic activity for long-chain natural and synthetic lipids. HPLC analysis showed that this enzyme has 1,3 regioselectivity when Glycerol tripalmitate and oleic acid are used as substrates. The fatty acid composition in the reaction product was 21.3% oleic acid and 79.1% sn-2 palmitic acid.