The Experts below are selected from a list of 51 Experts worldwide ranked by ideXlab platform
Marianne Van Hagehamsten - One of the best experts on this subject based on the ideXlab platform.
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cross reactivity studies of a new group 2 allergen from the dust mite Glycyphagus domesticus gly d 2 and group 2 allergens from dermatophagoides pteronyssinus lepidoglyphus destructor and tyrophagus putrescentiae with recombinant allergens
The Journal of Allergy and Clinical Immunology, 2001Co-Authors: Guro Gafvelin, Eva Johansson, Anna Lundin, Alisa M Smith, Martin D Chapman, David C Benjamin, Urszula Derewenda, Marianne Van HagehamstenAbstract:Abstract Background: Dust mites are important inducers of allergic disease. Group 2 allergens are recognized as major allergens in several mite species, including Dermatophagoides pteronys-sinus , Lepidoglyphus destructor , and Tyrophagus putrescentiae . No allergens have thus far been characterized on the molecular level from the dust mite Glycyphagus domesticus . Objective: We sought to examine the cross-reactivity among group 2 allergens of G domesticus , L destructor , T putrescentiae , and D pteronyssinus . Methods: A group 2 allergen from G domesticus , Gly d 2, was cloned and expressed as a recombinant protein. Cross-reactivity between Gly d 2 and 3 other group 2 allergens, Lep d 2, Tyr p 2, and Der p 2, was studied by using individual sera and a serum pool RAST-positive to G domesticus , L destructor , T putrescentiae , and D pteronyssinus . Recombinant allergens were used as inhibitors of IgE binding in immunoblotting experiments. Molecular modeling on the basis of the Der p 2 structure was carried out for Gly d 2, Lep d 2, and Tyr p 2. Results: Two cDNAs encoding isoforms of Gly d 2 were isolated, but only the Gly d 2.02 isoform was used in this study. Sixteen of 17 subjects had IgE to Gly d 2. The protein sequence of Gly d 2 revealed 79% identity to Lep d 2 and 46% and 41% identity to Tyr p 2 and Der p 2, respectively. Extensive cross-reactivity was demonstrated among Gly d 2, Lep d 2, and Tyr p 2, but little cross-reactivity was found between these allergens and Der p 2. According to the tertiary structure of Der p 2 and 3-dimensional models of Gly d 2, Lep d 2, and Tyr p 2, differences reside mainly in surface-exposed residues. Conclusion: Gly d 2 showed high sequence homology to Lep d 2. Cross-reactivity was observed between Gly d 2, Lep d 2, and Tyr p 2, but only limited cross-reactivity was demonstrated between these 3 allergens and Der p 2. (J Allergy Clin Immunol 2001;107:511-8.)
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clinical and immunologic aspects of storage mite allergy
Allergy, 1998Co-Authors: Marianne Van Hagehamsten, E. JohanssonAbstract:: Exposure to storage (nonpyroglyphid) mites has been increasingly recognized as a cause of asthma and rhinitis. Several species have been identified in Europe; e.g., Lepidoglyphus destructor, Acarus farris/siro, Tyrophagus spp., Glycyphagus domesticus, and Blomia tjibodas. Blomia tropicalis, on the other hand, predominates in subtropical and tropical areas. Studies from several countries have shown that IgE-mediated allergy in rural populations is of considerable importance and that storage mites are major allergens. Since these mites are found in homes, especially in regions with damp housing conditions, urban populations are at risk of becoming sensitized. Therefore, sensitization is not restricted only to those with occupational exposure. Several major allergens from storage mites have been identified and characterized. There seems to be a limited allergenic cross-reactivity between storage- and house dust mites. Molecular cloning has demonstrated that the Group 2 allergens from storage mites (Lep d 2 and Tyr p 2) show more than 40% sequence identity with the Group 2 allergens from Dermatophagoides spp. The availability of a large number of recombinant dust mite allergens will make it possible to investigate their homology and the number of allergens required for the diagnosis and treatment of storage mite allergy.
E. Johansson - One of the best experts on this subject based on the ideXlab platform.
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clinical and immunologic aspects of storage mite allergy
Allergy, 1998Co-Authors: Marianne Van Hagehamsten, E. JohanssonAbstract:: Exposure to storage (nonpyroglyphid) mites has been increasingly recognized as a cause of asthma and rhinitis. Several species have been identified in Europe; e.g., Lepidoglyphus destructor, Acarus farris/siro, Tyrophagus spp., Glycyphagus domesticus, and Blomia tjibodas. Blomia tropicalis, on the other hand, predominates in subtropical and tropical areas. Studies from several countries have shown that IgE-mediated allergy in rural populations is of considerable importance and that storage mites are major allergens. Since these mites are found in homes, especially in regions with damp housing conditions, urban populations are at risk of becoming sensitized. Therefore, sensitization is not restricted only to those with occupational exposure. Several major allergens from storage mites have been identified and characterized. There seems to be a limited allergenic cross-reactivity between storage- and house dust mites. Molecular cloning has demonstrated that the Group 2 allergens from storage mites (Lep d 2 and Tyr p 2) show more than 40% sequence identity with the Group 2 allergens from Dermatophagoides spp. The availability of a large number of recombinant dust mite allergens will make it possible to investigate their homology and the number of allergens required for the diagnosis and treatment of storage mite allergy.
D Barber - One of the best experts on this subject based on the ideXlab platform.
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limited ige cross reactivity between dermatophagoides pteronyssinus and Glycyphagus domesticus in patients naturally exposed to both mite species
The Journal of Allergy and Clinical Immunology, 2007Co-Authors: Jose Ariasirigoyen, M Lombardero, C Arteaga, J A Carpizo, D BarberAbstract:Background Contradictory results have been found when analyzing the IgE cross-reactivity among pyroglyphid mites and storage mites, as well as the role of these as true sensitizing agents. Objective We sought to study the prevalence of sensitization to Glycyphagus domesticus in patients naturally exposed to this mite together with the more ubiquitous Dermatophagoides pteronyssinus and the IgE cross-reactivity between them. Methods Mite species present in house dust samples of consecutive patients with mite allergy from Huelva (southwest Spain) were determined by means of light microscopy. Clinical sensitization was assessed by using skin prick and conjunctival provocation tests. Cross-reactivity at the IgE level was studied by using serum IgE determination and inhibition of RAST, IgE immunoblotting, and mite group 2 immunoassays. Results After D pteronyssinus , which is present in about 95% of house dust samples, G domesticus was the most important mite and present in about 50% of the samples. Tyrophagus putrescentiae and Lepidoglyphus destructor were detected in third and fourth place, respectively. About half of the patients with G domesticus at home were sensitized to this mite. A low IgE cross-reactivity was observed between D pteronyssinus and G domesticus , but an important IgE cross-reactivity was detected among glycyphagid mites at the level of group 2 allergens. Conclusion Glycyphagid mites can act as primary sensitizing agents independently of pyroglyphid mites in a subset of patients naturally exposed to them. Clinical implications The inclusion of glycyphagid mite extracts in the diagnostic battery in areas with adequate mite growing conditions is important to ensure proper diagnosis.
Guro Gafvelin - One of the best experts on this subject based on the ideXlab platform.
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cross reactivity studies of a new group 2 allergen from the dust mite Glycyphagus domesticus gly d 2 and group 2 allergens from dermatophagoides pteronyssinus lepidoglyphus destructor and tyrophagus putrescentiae with recombinant allergens
The Journal of Allergy and Clinical Immunology, 2001Co-Authors: Guro Gafvelin, Eva Johansson, Anna Lundin, Alisa M Smith, Martin D Chapman, David C Benjamin, Urszula Derewenda, Marianne Van HagehamstenAbstract:Abstract Background: Dust mites are important inducers of allergic disease. Group 2 allergens are recognized as major allergens in several mite species, including Dermatophagoides pteronys-sinus , Lepidoglyphus destructor , and Tyrophagus putrescentiae . No allergens have thus far been characterized on the molecular level from the dust mite Glycyphagus domesticus . Objective: We sought to examine the cross-reactivity among group 2 allergens of G domesticus , L destructor , T putrescentiae , and D pteronyssinus . Methods: A group 2 allergen from G domesticus , Gly d 2, was cloned and expressed as a recombinant protein. Cross-reactivity between Gly d 2 and 3 other group 2 allergens, Lep d 2, Tyr p 2, and Der p 2, was studied by using individual sera and a serum pool RAST-positive to G domesticus , L destructor , T putrescentiae , and D pteronyssinus . Recombinant allergens were used as inhibitors of IgE binding in immunoblotting experiments. Molecular modeling on the basis of the Der p 2 structure was carried out for Gly d 2, Lep d 2, and Tyr p 2. Results: Two cDNAs encoding isoforms of Gly d 2 were isolated, but only the Gly d 2.02 isoform was used in this study. Sixteen of 17 subjects had IgE to Gly d 2. The protein sequence of Gly d 2 revealed 79% identity to Lep d 2 and 46% and 41% identity to Tyr p 2 and Der p 2, respectively. Extensive cross-reactivity was demonstrated among Gly d 2, Lep d 2, and Tyr p 2, but little cross-reactivity was found between these allergens and Der p 2. According to the tertiary structure of Der p 2 and 3-dimensional models of Gly d 2, Lep d 2, and Tyr p 2, differences reside mainly in surface-exposed residues. Conclusion: Gly d 2 showed high sequence homology to Lep d 2. Cross-reactivity was observed between Gly d 2, Lep d 2, and Tyr p 2, but only limited cross-reactivity was demonstrated between these 3 allergens and Der p 2. (J Allergy Clin Immunol 2001;107:511-8.)
Jose Ariasirigoyen - One of the best experts on this subject based on the ideXlab platform.
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limited ige cross reactivity between dermatophagoides pteronyssinus and Glycyphagus domesticus in patients naturally exposed to both mite species
The Journal of Allergy and Clinical Immunology, 2007Co-Authors: Jose Ariasirigoyen, M Lombardero, C Arteaga, J A Carpizo, D BarberAbstract:Background Contradictory results have been found when analyzing the IgE cross-reactivity among pyroglyphid mites and storage mites, as well as the role of these as true sensitizing agents. Objective We sought to study the prevalence of sensitization to Glycyphagus domesticus in patients naturally exposed to this mite together with the more ubiquitous Dermatophagoides pteronyssinus and the IgE cross-reactivity between them. Methods Mite species present in house dust samples of consecutive patients with mite allergy from Huelva (southwest Spain) were determined by means of light microscopy. Clinical sensitization was assessed by using skin prick and conjunctival provocation tests. Cross-reactivity at the IgE level was studied by using serum IgE determination and inhibition of RAST, IgE immunoblotting, and mite group 2 immunoassays. Results After D pteronyssinus , which is present in about 95% of house dust samples, G domesticus was the most important mite and present in about 50% of the samples. Tyrophagus putrescentiae and Lepidoglyphus destructor were detected in third and fourth place, respectively. About half of the patients with G domesticus at home were sensitized to this mite. A low IgE cross-reactivity was observed between D pteronyssinus and G domesticus , but an important IgE cross-reactivity was detected among glycyphagid mites at the level of group 2 allergens. Conclusion Glycyphagid mites can act as primary sensitizing agents independently of pyroglyphid mites in a subset of patients naturally exposed to them. Clinical implications The inclusion of glycyphagid mite extracts in the diagnostic battery in areas with adequate mite growing conditions is important to ensure proper diagnosis.