The Experts below are selected from a list of 315 Experts worldwide ranked by ideXlab platform
Yanhu Wang - One of the best experts on this subject based on the ideXlab platform.
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Silencing MAP3K1 expression inhibits the proliferation of Goat Hair follicle stem cells.
In vitro cellular & developmental biology. Animal, 2021Co-Authors: Jian Wang, Liuming Zhang, Changjiang Chu, Yunkui Feng, Qiang Wang, Yanhu WangAbstract:The Yangtze River Delta White Goat is the only Goat breed in the world that can produce superior-quality brush Hair. Previous studies have shown that some genes are expressed differentially in the skin tissues between the Goats produced superior-quality and normal-quality brush Hair. Studies also have shown that different gene play varied roles in regulating the proliferation and apoptosis of Hair follicle stem cells. However, the biological function of MAP3K1 (mitogen-activated protein kinase kinase kinase 1) gene in Hair follicle stem cells is not fully understood. This study aims to investigate the role of MAP3K1 knockdown during the proliferation and apoptosis of Hair follicle stem cells. RT-qPCR and Western blot were used to detect mRNA gene and protein expression level, CCK-8 and EdU assays were used to detect cell proliferation, and cell cycle and apoptosis were detected by flow cytometry. The results showed that the MAP3K1 expression level was significantly higher in the skin tissue of produced superior-quality brush Hair than that in produced normal-quality brush Hair. Moreover, functional studies indicated that si-MAP3K1 significantly inhibits the proliferation of Hair follicle stem cells that came from a superior Goat and promotes its apoptosis. Based on aforementioned assays, we speculated that MAP3K1 might play a regulatory effect in superior-quality brush Hair traits.
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mir 31 5p promotes proliferation and inhibits apoptosis of Goat Hair follicle stem cells by targeting rasa1 map3k1 pathway
Experimental Cell Research, 2021Co-Authors: Yunkui Feng, Jian Wang, Liuming Zhang, Changjiang Chu, Yanhu WangAbstract:Abstract The Yangtze River Delta white Goat is a sole Goat species that can naturally produce superior-quality brush Hair. It's worth to mention that study the developmental mechanism of Goat Hair follicle stem cells is vital for future breed preservation and molecular breeding. In this study, we successfully isolated Hair follicle stem cells from the skin tissue of fetal sheep neck spine, and harvested superior-quality and normal-quality brush Hair Goat tissue. The expression of miR-31-5p in Goat Hair follicle stem cells was verified by qPCR and Western blot. The effects of overexpression or inhibition of miR-31-5p on the proliferation and apoptosis of Hair follicle stem cells were detected by EdU, CCK-8, flow cytometry, etc. miR-31-5p can significantly improve cell proliferation and inhibit cell apoptosis by targeting RASA1 and upregulating MAP3K1 level, whereas miR-31-5p knockdown led to an opposite effect. These results reveal a miR-31-5p-associated regulatory network between miR-31-5p and RASA1/MAP3K1 during the progression of superiorquality brush Hair traits.
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miR-31-5p promotes proliferation and inhibits apoptosis of Goat Hair follicle stem cells by targeting RASA1/MAP3K1 pathway.
Experimental cell research, 2020Co-Authors: Feng Yunkui, Jian Wang, Ma Jinliang, Liuming Zhang, Changjiang Chu, Yanhu Wang, Li YongjunAbstract:Abstract The Yangtze River Delta white Goat is a sole Goat species that can naturally produce superior-quality brush Hair. It's worth to mention that study the developmental mechanism of Goat Hair follicle stem cells is vital for future breed preservation and molecular breeding. In this study, we successfully isolated Hair follicle stem cells from the skin tissue of fetal sheep neck spine, and harvested superior-quality and normal-quality brush Hair Goat tissue. The expression of miR-31-5p in Goat Hair follicle stem cells was verified by qPCR and Western blot. The effects of overexpression or inhibition of miR-31-5p on the proliferation and apoptosis of Hair follicle stem cells were detected by EdU, CCK-8, flow cytometry, etc. miR-31-5p can significantly improve cell proliferation and inhibit cell apoptosis by targeting RASA1 and upregulating MAP3K1 level, whereas miR-31-5p knockdown led to an opposite effect. These results reveal a miR-31-5p-associated regulatory network between miR-31-5p and RASA1/MAP3K1 during the progression of superiorquality brush Hair traits.
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miR-149-5p Regulates Goat Hair Follicle Stem Cell Proliferation and Apoptosis by Targeting the CMTM3/AR Axis During Superior-Quality Brush Hair Formation.
Frontiers in genetics, 2020Co-Authors: Jian Wang, Feng Yunkui, Ma Jinliang, Liuming Zhang, Changjiang Chu, Li Yongjun, Qu Jingwen, Yanhu WangAbstract:The Yangtze River Delta white Goat is a unique Goat species that can produce superior quality brush Hair. CKLF-like MARVEL transmembrane domain-containing 3 (CMTM3), which influences the transcriptional activity of androgen receptor (AR), was identified as a candidate gene related to superior-quality brush Hair formation. CMTM3 is generally expressed at low levels, but miR-149-5p is highly expressed in the skin tissues of these Goats. The mechanism by which CMTM3 regulates the proliferation and apoptosis of Goat Hair follicle stem cells has not been elucidated. Here, RT-qPCR, western blotting, 5-ethynyl-2'-deoxyuridine (EdU), cell cycle, apoptosis, and dual-luciferase assays were used to investigate the role and regulatory mechanism of CMTM3 and miR-149-5p. Functional studies showed that CMTM3 overexpression inhibited proliferation and induced apoptosis in cultured Hair follicle stem cells, whereas silencing CMTM3 markedly facilitated cell proliferation and deterred apoptosis in cultured Hair follicle stem cells. Then, using bioinformatic predictions and the aforementioned assays, including dual-luciferase assays, RT-qPCR, and western blotting, we confirmed that miR-149-5p targets CMTM3 and preliminarily investigated the interaction between CMTM3 and AR in Goat Hair follicle stem cells. Furthermore, miR-149-5p overexpression significantly accelerated the proliferation and attenuated the apoptosis of Hair follicle stem cells. Conversely, miR-149-5p inhibition suppressed the proliferation and induced the apoptosis of Hair follicle stem cells. These results reveal a miR-149-5p-related regulatory framework for the miR-149-5p/CMTM3/AR axis during superior quality brush Hair formation, in which CMTM3 plays a negative role.
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mir 149 5p regulates Goat Hair follicle stem cell proliferation and apoptosis by targeting the cmtm3 ar axis during superior quality brush Hair formation
Frontiers in Genetics, 2020Co-Authors: Jian Wang, Liuming Zhang, Changjiang Chu, Yunkui Feng, Yanhu WangAbstract:The Yangtze River Delta white Goat is a unique Goat species that can produce superior quality brush Hair. CKLF-like MARVEL transmembrane domain-containing 3 (CMTM3), which influences the transcriptional activity of androgen receptor (AR), was identified as a candidate gene related to superior-quality brush Hair formation. CMTM3 is generally expressed at low levels, but miR-149-5p is highly expressed in the skin tissues of these Goats. The mechanism by which CMTM3 regulates the proliferation and apoptosis of Goat Hair follicle stem cells has not been elucidated. Here, RT-qPCR, western blotting, 5-ethynyl-2'-deoxyuridine (EdU), cell cycle, apoptosis, and dual-luciferase assays were used to investigate the role and regulatory mechanism of CMTM3 and miR-149-5p. Functional studies showed that CMTM3 overexpression inhibited proliferation and induced apoptosis in cultured Hair follicle stem cells, whereas silencing CMTM3 markedly facilitated cell proliferation and deterred apoptosis in cultured Hair follicle stem cells. Then, using bioinformatic predictions and the aforementioned assays, including dual-luciferase assays, RT-qPCR, and western blotting, we confirmed that miR-149-5p targets CMTM3 and preliminarily investigated the interaction between CMTM3 and AR in Goat Hair follicle stem cells. Furthermore, miR-149-5p overexpression significantly accelerated the proliferation and attenuated the apoptosis of Hair follicle stem cells. Conversely, miR-149-5p inhibition suppressed the proliferation and induced the apoptosis of Hair follicle stem cells. These results reveal a miR-149-5p-related regulatory framework for the miR-149-5p/CMTM3/AR axis during superior quality brush Hair formation, in which CMTM3 plays a negative role.
Jian Wang - One of the best experts on this subject based on the ideXlab platform.
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Silencing MAP3K1 expression inhibits the proliferation of Goat Hair follicle stem cells.
In vitro cellular & developmental biology. Animal, 2021Co-Authors: Jian Wang, Liuming Zhang, Changjiang Chu, Yunkui Feng, Qiang Wang, Yanhu WangAbstract:The Yangtze River Delta White Goat is the only Goat breed in the world that can produce superior-quality brush Hair. Previous studies have shown that some genes are expressed differentially in the skin tissues between the Goats produced superior-quality and normal-quality brush Hair. Studies also have shown that different gene play varied roles in regulating the proliferation and apoptosis of Hair follicle stem cells. However, the biological function of MAP3K1 (mitogen-activated protein kinase kinase kinase 1) gene in Hair follicle stem cells is not fully understood. This study aims to investigate the role of MAP3K1 knockdown during the proliferation and apoptosis of Hair follicle stem cells. RT-qPCR and Western blot were used to detect mRNA gene and protein expression level, CCK-8 and EdU assays were used to detect cell proliferation, and cell cycle and apoptosis were detected by flow cytometry. The results showed that the MAP3K1 expression level was significantly higher in the skin tissue of produced superior-quality brush Hair than that in produced normal-quality brush Hair. Moreover, functional studies indicated that si-MAP3K1 significantly inhibits the proliferation of Hair follicle stem cells that came from a superior Goat and promotes its apoptosis. Based on aforementioned assays, we speculated that MAP3K1 might play a regulatory effect in superior-quality brush Hair traits.
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mir 31 5p promotes proliferation and inhibits apoptosis of Goat Hair follicle stem cells by targeting rasa1 map3k1 pathway
Experimental Cell Research, 2021Co-Authors: Yunkui Feng, Jian Wang, Liuming Zhang, Changjiang Chu, Yanhu WangAbstract:Abstract The Yangtze River Delta white Goat is a sole Goat species that can naturally produce superior-quality brush Hair. It's worth to mention that study the developmental mechanism of Goat Hair follicle stem cells is vital for future breed preservation and molecular breeding. In this study, we successfully isolated Hair follicle stem cells from the skin tissue of fetal sheep neck spine, and harvested superior-quality and normal-quality brush Hair Goat tissue. The expression of miR-31-5p in Goat Hair follicle stem cells was verified by qPCR and Western blot. The effects of overexpression or inhibition of miR-31-5p on the proliferation and apoptosis of Hair follicle stem cells were detected by EdU, CCK-8, flow cytometry, etc. miR-31-5p can significantly improve cell proliferation and inhibit cell apoptosis by targeting RASA1 and upregulating MAP3K1 level, whereas miR-31-5p knockdown led to an opposite effect. These results reveal a miR-31-5p-associated regulatory network between miR-31-5p and RASA1/MAP3K1 during the progression of superiorquality brush Hair traits.
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miR-31-5p promotes proliferation and inhibits apoptosis of Goat Hair follicle stem cells by targeting RASA1/MAP3K1 pathway.
Experimental cell research, 2020Co-Authors: Feng Yunkui, Jian Wang, Ma Jinliang, Liuming Zhang, Changjiang Chu, Yanhu Wang, Li YongjunAbstract:Abstract The Yangtze River Delta white Goat is a sole Goat species that can naturally produce superior-quality brush Hair. It's worth to mention that study the developmental mechanism of Goat Hair follicle stem cells is vital for future breed preservation and molecular breeding. In this study, we successfully isolated Hair follicle stem cells from the skin tissue of fetal sheep neck spine, and harvested superior-quality and normal-quality brush Hair Goat tissue. The expression of miR-31-5p in Goat Hair follicle stem cells was verified by qPCR and Western blot. The effects of overexpression or inhibition of miR-31-5p on the proliferation and apoptosis of Hair follicle stem cells were detected by EdU, CCK-8, flow cytometry, etc. miR-31-5p can significantly improve cell proliferation and inhibit cell apoptosis by targeting RASA1 and upregulating MAP3K1 level, whereas miR-31-5p knockdown led to an opposite effect. These results reveal a miR-31-5p-associated regulatory network between miR-31-5p and RASA1/MAP3K1 during the progression of superiorquality brush Hair traits.
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miR-149-5p Regulates Goat Hair Follicle Stem Cell Proliferation and Apoptosis by Targeting the CMTM3/AR Axis During Superior-Quality Brush Hair Formation.
Frontiers in genetics, 2020Co-Authors: Jian Wang, Feng Yunkui, Ma Jinliang, Liuming Zhang, Changjiang Chu, Li Yongjun, Qu Jingwen, Yanhu WangAbstract:The Yangtze River Delta white Goat is a unique Goat species that can produce superior quality brush Hair. CKLF-like MARVEL transmembrane domain-containing 3 (CMTM3), which influences the transcriptional activity of androgen receptor (AR), was identified as a candidate gene related to superior-quality brush Hair formation. CMTM3 is generally expressed at low levels, but miR-149-5p is highly expressed in the skin tissues of these Goats. The mechanism by which CMTM3 regulates the proliferation and apoptosis of Goat Hair follicle stem cells has not been elucidated. Here, RT-qPCR, western blotting, 5-ethynyl-2'-deoxyuridine (EdU), cell cycle, apoptosis, and dual-luciferase assays were used to investigate the role and regulatory mechanism of CMTM3 and miR-149-5p. Functional studies showed that CMTM3 overexpression inhibited proliferation and induced apoptosis in cultured Hair follicle stem cells, whereas silencing CMTM3 markedly facilitated cell proliferation and deterred apoptosis in cultured Hair follicle stem cells. Then, using bioinformatic predictions and the aforementioned assays, including dual-luciferase assays, RT-qPCR, and western blotting, we confirmed that miR-149-5p targets CMTM3 and preliminarily investigated the interaction between CMTM3 and AR in Goat Hair follicle stem cells. Furthermore, miR-149-5p overexpression significantly accelerated the proliferation and attenuated the apoptosis of Hair follicle stem cells. Conversely, miR-149-5p inhibition suppressed the proliferation and induced the apoptosis of Hair follicle stem cells. These results reveal a miR-149-5p-related regulatory framework for the miR-149-5p/CMTM3/AR axis during superior quality brush Hair formation, in which CMTM3 plays a negative role.
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mir 149 5p regulates Goat Hair follicle stem cell proliferation and apoptosis by targeting the cmtm3 ar axis during superior quality brush Hair formation
Frontiers in Genetics, 2020Co-Authors: Jian Wang, Liuming Zhang, Changjiang Chu, Yunkui Feng, Yanhu WangAbstract:The Yangtze River Delta white Goat is a unique Goat species that can produce superior quality brush Hair. CKLF-like MARVEL transmembrane domain-containing 3 (CMTM3), which influences the transcriptional activity of androgen receptor (AR), was identified as a candidate gene related to superior-quality brush Hair formation. CMTM3 is generally expressed at low levels, but miR-149-5p is highly expressed in the skin tissues of these Goats. The mechanism by which CMTM3 regulates the proliferation and apoptosis of Goat Hair follicle stem cells has not been elucidated. Here, RT-qPCR, western blotting, 5-ethynyl-2'-deoxyuridine (EdU), cell cycle, apoptosis, and dual-luciferase assays were used to investigate the role and regulatory mechanism of CMTM3 and miR-149-5p. Functional studies showed that CMTM3 overexpression inhibited proliferation and induced apoptosis in cultured Hair follicle stem cells, whereas silencing CMTM3 markedly facilitated cell proliferation and deterred apoptosis in cultured Hair follicle stem cells. Then, using bioinformatic predictions and the aforementioned assays, including dual-luciferase assays, RT-qPCR, and western blotting, we confirmed that miR-149-5p targets CMTM3 and preliminarily investigated the interaction between CMTM3 and AR in Goat Hair follicle stem cells. Furthermore, miR-149-5p overexpression significantly accelerated the proliferation and attenuated the apoptosis of Hair follicle stem cells. Conversely, miR-149-5p inhibition suppressed the proliferation and induced the apoptosis of Hair follicle stem cells. These results reveal a miR-149-5p-related regulatory framework for the miR-149-5p/CMTM3/AR axis during superior quality brush Hair formation, in which CMTM3 plays a negative role.
Liuming Zhang - One of the best experts on this subject based on the ideXlab platform.
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Silencing MAP3K1 expression inhibits the proliferation of Goat Hair follicle stem cells.
In vitro cellular & developmental biology. Animal, 2021Co-Authors: Jian Wang, Liuming Zhang, Changjiang Chu, Yunkui Feng, Qiang Wang, Yanhu WangAbstract:The Yangtze River Delta White Goat is the only Goat breed in the world that can produce superior-quality brush Hair. Previous studies have shown that some genes are expressed differentially in the skin tissues between the Goats produced superior-quality and normal-quality brush Hair. Studies also have shown that different gene play varied roles in regulating the proliferation and apoptosis of Hair follicle stem cells. However, the biological function of MAP3K1 (mitogen-activated protein kinase kinase kinase 1) gene in Hair follicle stem cells is not fully understood. This study aims to investigate the role of MAP3K1 knockdown during the proliferation and apoptosis of Hair follicle stem cells. RT-qPCR and Western blot were used to detect mRNA gene and protein expression level, CCK-8 and EdU assays were used to detect cell proliferation, and cell cycle and apoptosis were detected by flow cytometry. The results showed that the MAP3K1 expression level was significantly higher in the skin tissue of produced superior-quality brush Hair than that in produced normal-quality brush Hair. Moreover, functional studies indicated that si-MAP3K1 significantly inhibits the proliferation of Hair follicle stem cells that came from a superior Goat and promotes its apoptosis. Based on aforementioned assays, we speculated that MAP3K1 might play a regulatory effect in superior-quality brush Hair traits.
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mir 31 5p promotes proliferation and inhibits apoptosis of Goat Hair follicle stem cells by targeting rasa1 map3k1 pathway
Experimental Cell Research, 2021Co-Authors: Yunkui Feng, Jian Wang, Liuming Zhang, Changjiang Chu, Yanhu WangAbstract:Abstract The Yangtze River Delta white Goat is a sole Goat species that can naturally produce superior-quality brush Hair. It's worth to mention that study the developmental mechanism of Goat Hair follicle stem cells is vital for future breed preservation and molecular breeding. In this study, we successfully isolated Hair follicle stem cells from the skin tissue of fetal sheep neck spine, and harvested superior-quality and normal-quality brush Hair Goat tissue. The expression of miR-31-5p in Goat Hair follicle stem cells was verified by qPCR and Western blot. The effects of overexpression or inhibition of miR-31-5p on the proliferation and apoptosis of Hair follicle stem cells were detected by EdU, CCK-8, flow cytometry, etc. miR-31-5p can significantly improve cell proliferation and inhibit cell apoptosis by targeting RASA1 and upregulating MAP3K1 level, whereas miR-31-5p knockdown led to an opposite effect. These results reveal a miR-31-5p-associated regulatory network between miR-31-5p and RASA1/MAP3K1 during the progression of superiorquality brush Hair traits.
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miR-31-5p promotes proliferation and inhibits apoptosis of Goat Hair follicle stem cells by targeting RASA1/MAP3K1 pathway.
Experimental cell research, 2020Co-Authors: Feng Yunkui, Jian Wang, Ma Jinliang, Liuming Zhang, Changjiang Chu, Yanhu Wang, Li YongjunAbstract:Abstract The Yangtze River Delta white Goat is a sole Goat species that can naturally produce superior-quality brush Hair. It's worth to mention that study the developmental mechanism of Goat Hair follicle stem cells is vital for future breed preservation and molecular breeding. In this study, we successfully isolated Hair follicle stem cells from the skin tissue of fetal sheep neck spine, and harvested superior-quality and normal-quality brush Hair Goat tissue. The expression of miR-31-5p in Goat Hair follicle stem cells was verified by qPCR and Western blot. The effects of overexpression or inhibition of miR-31-5p on the proliferation and apoptosis of Hair follicle stem cells were detected by EdU, CCK-8, flow cytometry, etc. miR-31-5p can significantly improve cell proliferation and inhibit cell apoptosis by targeting RASA1 and upregulating MAP3K1 level, whereas miR-31-5p knockdown led to an opposite effect. These results reveal a miR-31-5p-associated regulatory network between miR-31-5p and RASA1/MAP3K1 during the progression of superiorquality brush Hair traits.
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miR-149-5p Regulates Goat Hair Follicle Stem Cell Proliferation and Apoptosis by Targeting the CMTM3/AR Axis During Superior-Quality Brush Hair Formation.
Frontiers in genetics, 2020Co-Authors: Jian Wang, Feng Yunkui, Ma Jinliang, Liuming Zhang, Changjiang Chu, Li Yongjun, Qu Jingwen, Yanhu WangAbstract:The Yangtze River Delta white Goat is a unique Goat species that can produce superior quality brush Hair. CKLF-like MARVEL transmembrane domain-containing 3 (CMTM3), which influences the transcriptional activity of androgen receptor (AR), was identified as a candidate gene related to superior-quality brush Hair formation. CMTM3 is generally expressed at low levels, but miR-149-5p is highly expressed in the skin tissues of these Goats. The mechanism by which CMTM3 regulates the proliferation and apoptosis of Goat Hair follicle stem cells has not been elucidated. Here, RT-qPCR, western blotting, 5-ethynyl-2'-deoxyuridine (EdU), cell cycle, apoptosis, and dual-luciferase assays were used to investigate the role and regulatory mechanism of CMTM3 and miR-149-5p. Functional studies showed that CMTM3 overexpression inhibited proliferation and induced apoptosis in cultured Hair follicle stem cells, whereas silencing CMTM3 markedly facilitated cell proliferation and deterred apoptosis in cultured Hair follicle stem cells. Then, using bioinformatic predictions and the aforementioned assays, including dual-luciferase assays, RT-qPCR, and western blotting, we confirmed that miR-149-5p targets CMTM3 and preliminarily investigated the interaction between CMTM3 and AR in Goat Hair follicle stem cells. Furthermore, miR-149-5p overexpression significantly accelerated the proliferation and attenuated the apoptosis of Hair follicle stem cells. Conversely, miR-149-5p inhibition suppressed the proliferation and induced the apoptosis of Hair follicle stem cells. These results reveal a miR-149-5p-related regulatory framework for the miR-149-5p/CMTM3/AR axis during superior quality brush Hair formation, in which CMTM3 plays a negative role.
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mir 149 5p regulates Goat Hair follicle stem cell proliferation and apoptosis by targeting the cmtm3 ar axis during superior quality brush Hair formation
Frontiers in Genetics, 2020Co-Authors: Jian Wang, Liuming Zhang, Changjiang Chu, Yunkui Feng, Yanhu WangAbstract:The Yangtze River Delta white Goat is a unique Goat species that can produce superior quality brush Hair. CKLF-like MARVEL transmembrane domain-containing 3 (CMTM3), which influences the transcriptional activity of androgen receptor (AR), was identified as a candidate gene related to superior-quality brush Hair formation. CMTM3 is generally expressed at low levels, but miR-149-5p is highly expressed in the skin tissues of these Goats. The mechanism by which CMTM3 regulates the proliferation and apoptosis of Goat Hair follicle stem cells has not been elucidated. Here, RT-qPCR, western blotting, 5-ethynyl-2'-deoxyuridine (EdU), cell cycle, apoptosis, and dual-luciferase assays were used to investigate the role and regulatory mechanism of CMTM3 and miR-149-5p. Functional studies showed that CMTM3 overexpression inhibited proliferation and induced apoptosis in cultured Hair follicle stem cells, whereas silencing CMTM3 markedly facilitated cell proliferation and deterred apoptosis in cultured Hair follicle stem cells. Then, using bioinformatic predictions and the aforementioned assays, including dual-luciferase assays, RT-qPCR, and western blotting, we confirmed that miR-149-5p targets CMTM3 and preliminarily investigated the interaction between CMTM3 and AR in Goat Hair follicle stem cells. Furthermore, miR-149-5p overexpression significantly accelerated the proliferation and attenuated the apoptosis of Hair follicle stem cells. Conversely, miR-149-5p inhibition suppressed the proliferation and induced the apoptosis of Hair follicle stem cells. These results reveal a miR-149-5p-related regulatory framework for the miR-149-5p/CMTM3/AR axis during superior quality brush Hair formation, in which CMTM3 plays a negative role.
Changjiang Chu - One of the best experts on this subject based on the ideXlab platform.
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Silencing MAP3K1 expression inhibits the proliferation of Goat Hair follicle stem cells.
In vitro cellular & developmental biology. Animal, 2021Co-Authors: Jian Wang, Liuming Zhang, Changjiang Chu, Yunkui Feng, Qiang Wang, Yanhu WangAbstract:The Yangtze River Delta White Goat is the only Goat breed in the world that can produce superior-quality brush Hair. Previous studies have shown that some genes are expressed differentially in the skin tissues between the Goats produced superior-quality and normal-quality brush Hair. Studies also have shown that different gene play varied roles in regulating the proliferation and apoptosis of Hair follicle stem cells. However, the biological function of MAP3K1 (mitogen-activated protein kinase kinase kinase 1) gene in Hair follicle stem cells is not fully understood. This study aims to investigate the role of MAP3K1 knockdown during the proliferation and apoptosis of Hair follicle stem cells. RT-qPCR and Western blot were used to detect mRNA gene and protein expression level, CCK-8 and EdU assays were used to detect cell proliferation, and cell cycle and apoptosis were detected by flow cytometry. The results showed that the MAP3K1 expression level was significantly higher in the skin tissue of produced superior-quality brush Hair than that in produced normal-quality brush Hair. Moreover, functional studies indicated that si-MAP3K1 significantly inhibits the proliferation of Hair follicle stem cells that came from a superior Goat and promotes its apoptosis. Based on aforementioned assays, we speculated that MAP3K1 might play a regulatory effect in superior-quality brush Hair traits.
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mir 31 5p promotes proliferation and inhibits apoptosis of Goat Hair follicle stem cells by targeting rasa1 map3k1 pathway
Experimental Cell Research, 2021Co-Authors: Yunkui Feng, Jian Wang, Liuming Zhang, Changjiang Chu, Yanhu WangAbstract:Abstract The Yangtze River Delta white Goat is a sole Goat species that can naturally produce superior-quality brush Hair. It's worth to mention that study the developmental mechanism of Goat Hair follicle stem cells is vital for future breed preservation and molecular breeding. In this study, we successfully isolated Hair follicle stem cells from the skin tissue of fetal sheep neck spine, and harvested superior-quality and normal-quality brush Hair Goat tissue. The expression of miR-31-5p in Goat Hair follicle stem cells was verified by qPCR and Western blot. The effects of overexpression or inhibition of miR-31-5p on the proliferation and apoptosis of Hair follicle stem cells were detected by EdU, CCK-8, flow cytometry, etc. miR-31-5p can significantly improve cell proliferation and inhibit cell apoptosis by targeting RASA1 and upregulating MAP3K1 level, whereas miR-31-5p knockdown led to an opposite effect. These results reveal a miR-31-5p-associated regulatory network between miR-31-5p and RASA1/MAP3K1 during the progression of superiorquality brush Hair traits.
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miR-31-5p promotes proliferation and inhibits apoptosis of Goat Hair follicle stem cells by targeting RASA1/MAP3K1 pathway.
Experimental cell research, 2020Co-Authors: Feng Yunkui, Jian Wang, Ma Jinliang, Liuming Zhang, Changjiang Chu, Yanhu Wang, Li YongjunAbstract:Abstract The Yangtze River Delta white Goat is a sole Goat species that can naturally produce superior-quality brush Hair. It's worth to mention that study the developmental mechanism of Goat Hair follicle stem cells is vital for future breed preservation and molecular breeding. In this study, we successfully isolated Hair follicle stem cells from the skin tissue of fetal sheep neck spine, and harvested superior-quality and normal-quality brush Hair Goat tissue. The expression of miR-31-5p in Goat Hair follicle stem cells was verified by qPCR and Western blot. The effects of overexpression or inhibition of miR-31-5p on the proliferation and apoptosis of Hair follicle stem cells were detected by EdU, CCK-8, flow cytometry, etc. miR-31-5p can significantly improve cell proliferation and inhibit cell apoptosis by targeting RASA1 and upregulating MAP3K1 level, whereas miR-31-5p knockdown led to an opposite effect. These results reveal a miR-31-5p-associated regulatory network between miR-31-5p and RASA1/MAP3K1 during the progression of superiorquality brush Hair traits.
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miR-149-5p Regulates Goat Hair Follicle Stem Cell Proliferation and Apoptosis by Targeting the CMTM3/AR Axis During Superior-Quality Brush Hair Formation.
Frontiers in genetics, 2020Co-Authors: Jian Wang, Feng Yunkui, Ma Jinliang, Liuming Zhang, Changjiang Chu, Li Yongjun, Qu Jingwen, Yanhu WangAbstract:The Yangtze River Delta white Goat is a unique Goat species that can produce superior quality brush Hair. CKLF-like MARVEL transmembrane domain-containing 3 (CMTM3), which influences the transcriptional activity of androgen receptor (AR), was identified as a candidate gene related to superior-quality brush Hair formation. CMTM3 is generally expressed at low levels, but miR-149-5p is highly expressed in the skin tissues of these Goats. The mechanism by which CMTM3 regulates the proliferation and apoptosis of Goat Hair follicle stem cells has not been elucidated. Here, RT-qPCR, western blotting, 5-ethynyl-2'-deoxyuridine (EdU), cell cycle, apoptosis, and dual-luciferase assays were used to investigate the role and regulatory mechanism of CMTM3 and miR-149-5p. Functional studies showed that CMTM3 overexpression inhibited proliferation and induced apoptosis in cultured Hair follicle stem cells, whereas silencing CMTM3 markedly facilitated cell proliferation and deterred apoptosis in cultured Hair follicle stem cells. Then, using bioinformatic predictions and the aforementioned assays, including dual-luciferase assays, RT-qPCR, and western blotting, we confirmed that miR-149-5p targets CMTM3 and preliminarily investigated the interaction between CMTM3 and AR in Goat Hair follicle stem cells. Furthermore, miR-149-5p overexpression significantly accelerated the proliferation and attenuated the apoptosis of Hair follicle stem cells. Conversely, miR-149-5p inhibition suppressed the proliferation and induced the apoptosis of Hair follicle stem cells. These results reveal a miR-149-5p-related regulatory framework for the miR-149-5p/CMTM3/AR axis during superior quality brush Hair formation, in which CMTM3 plays a negative role.
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mir 149 5p regulates Goat Hair follicle stem cell proliferation and apoptosis by targeting the cmtm3 ar axis during superior quality brush Hair formation
Frontiers in Genetics, 2020Co-Authors: Jian Wang, Liuming Zhang, Changjiang Chu, Yunkui Feng, Yanhu WangAbstract:The Yangtze River Delta white Goat is a unique Goat species that can produce superior quality brush Hair. CKLF-like MARVEL transmembrane domain-containing 3 (CMTM3), which influences the transcriptional activity of androgen receptor (AR), was identified as a candidate gene related to superior-quality brush Hair formation. CMTM3 is generally expressed at low levels, but miR-149-5p is highly expressed in the skin tissues of these Goats. The mechanism by which CMTM3 regulates the proliferation and apoptosis of Goat Hair follicle stem cells has not been elucidated. Here, RT-qPCR, western blotting, 5-ethynyl-2'-deoxyuridine (EdU), cell cycle, apoptosis, and dual-luciferase assays were used to investigate the role and regulatory mechanism of CMTM3 and miR-149-5p. Functional studies showed that CMTM3 overexpression inhibited proliferation and induced apoptosis in cultured Hair follicle stem cells, whereas silencing CMTM3 markedly facilitated cell proliferation and deterred apoptosis in cultured Hair follicle stem cells. Then, using bioinformatic predictions and the aforementioned assays, including dual-luciferase assays, RT-qPCR, and western blotting, we confirmed that miR-149-5p targets CMTM3 and preliminarily investigated the interaction between CMTM3 and AR in Goat Hair follicle stem cells. Furthermore, miR-149-5p overexpression significantly accelerated the proliferation and attenuated the apoptosis of Hair follicle stem cells. Conversely, miR-149-5p inhibition suppressed the proliferation and induced the apoptosis of Hair follicle stem cells. These results reveal a miR-149-5p-related regulatory framework for the miR-149-5p/CMTM3/AR axis during superior quality brush Hair formation, in which CMTM3 plays a negative role.
Dunmin Lin - One of the best experts on this subject based on the ideXlab platform.
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Sulfur-encapsulated in heteroatom-doped hierarchical porous carbon derived from Goat Hair for high performance lithium–sulfur batteries
Journal of Energy Chemistry, 2019Co-Authors: Juan Ren, Yibei Zhou, Fengyu Xie, Dunmin LinAbstract:Abstract Biomass-derived carbon materials have aroused widespread concern as host material of sulfur to enhance electrochemical performances for lithium–sulfur batteries. Herein, Goat Hair, as a low-cost and eco-friendly precursor, is employed to fabricate cauliflower-like in-situ nitrogen, oxygen and phosphorus tri-doped porous biomass carbon (NOPC) by a facile activation with H3PO4 and carbonization process. The morphology and microstructure of NOPC can be readily tuned by altering pyrolysis temperature. The as-prepared NOPC matrix material carbonized at 600 °C possesses 3D hierarchical porous structure, high specific surface area (535.352 m2 g−1), and appropriate pore size and pore size distribution. Encapsulating sulfur into the NOPC depends on a stem-melting technology as cathode materials of Li–S batteries. Due to the synergistic effect of special physical structure and inherent tri-doping of N, O and P, electrons and ions transfer and utilization of active sulfur in the materials are improved, and the shuttle behaviors of soluble lithium polysulfides are also mitigated. Consequently, the S/NOPC-600 composite exhibits excellent electrochemical performance, giving a high initial discharge capacity of 1185 mA h g−1 at 0.05 C and maintaining a relatively considerable capacity of 489 mA h g−1 at 0.2 C after 300 cycles. Our work shows that a promising candidate for cathode material of Li–S batteries can be synthesized using low-cost and renewable biomass materials by a facile process.
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sulfur encapsulated in heteroatom doped hierarchical porous carbon derived from Goat Hair for high performance lithium sulfur batteries
Journal of Energy Chemistry, 2018Co-Authors: Juan Ren, Yibei Zhou, Fengyu Xie, Dunmin LinAbstract:Abstract Biomass-derived carbon materials have aroused widespread concern as host material of sulfur to enhance electrochemical performances for lithium–sulfur batteries. Herein, Goat Hair, as a low-cost and eco-friendly precursor, is employed to fabricate cauliflower-like in-situ nitrogen, oxygen and phosphorus tri-doped porous biomass carbon (NOPC) by a facile activation with H3PO4 and carbonization process. The morphology and microstructure of NOPC can be readily tuned by altering pyrolysis temperature. The as-prepared NOPC matrix material carbonized at 600 °C possesses 3D hierarchical porous structure, high specific surface area (535.352 m2 g−1), and appropriate pore size and pore size distribution. Encapsulating sulfur into the NOPC depends on a stem-melting technology as cathode materials of Li–S batteries. Due to the synergistic effect of special physical structure and inherent tri-doping of N, O and P, electrons and ions transfer and utilization of active sulfur in the materials are improved, and the shuttle behaviors of soluble lithium polysulfides are also mitigated. Consequently, the S/NOPC-600 composite exhibits excellent electrochemical performance, giving a high initial discharge capacity of 1185 mA h g−1 at 0.05 C and maintaining a relatively considerable capacity of 489 mA h g−1 at 0.2 C after 300 cycles. Our work shows that a promising candidate for cathode material of Li–S batteries can be synthesized using low-cost and renewable biomass materials by a facile process.