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Xianlong Zhang - One of the best experts on this subject based on the ideXlab platform.
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gbsobir1 confers verticillium wilt resistance by phosphorylating the transcriptional factor gbbhlh171 in Gossypium barbadense
Plant Biotechnology Journal, 2019Co-Authors: Yi Zhou, Longfu Zhu, Longqing Sun, Ghulam Mustafa Wassan, Muhammad Shaban, Lin Zhang, Xianlong ZhangAbstract:Receptor-like kinases (RLKs) are important components of plant innate immunity. Although recent studies have revealed that the RLK suppressor of BIR1-1 (SOBIR1) can interact with multiple receptor-like proteins and is required for resistance against fungal pathogens, how the signal is transduced and triggers immune responses remains enigmatic. In this study, we identified a defence-related RLK from Gossypium barbadense (designated GbSOBIR1) and investigated its functional mechanism. Expression of the GbSOBIR1 gene is ubiquitous in cotton plants and is induced by Verticillium dahliae inoculation. Knock-down of GbSOBIR1 by virus-induced gene silencing resulted in attenuated resistance of cotton plants to V. dahliae, while heterologous overexpression of GbSOBIR1 in Arabidopsis improves resistance. We also found that the kinase region of GbSOBIR1 interacts with a basic helix-loop-helix (bHLH) transcription factor identified as GbbHLH171 in a yeast-two-hybrid screen. GbbHLH171 could interact with and be phosphorylated by GbSOBIR1 in vitro and in vivo and contributes positively to the resistance of cotton against V. dahliae. Furthermore, we found that this phosphorylation is essential to the transcriptional activity and functional role of GbbHLH171. We also show by spectrometric analysis and site-directed mutagenesis that Ser413 is the GbSOBIR1-mediated phosphorylation site of GbbHLH171. These results demonstrate that GbSOBIR1 interacts with GbbHLH171 and plays a critical role in cotton resistance to V. dahliae.
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identification of multiple stress responsive genes by sequencing a normalized cdna library from sea land cotton Gossypium barbadense l
PLOS ONE, 2016Co-Authors: Bin Zhou, Lin Zhang, Abid Ullah, Xin Jin, Xiyan Yang, Xianlong ZhangAbstract:Background Plants often face multiple stresses including drought, extreme temperature, salinity, nutrition deficiency and biotic stresses during growth and development. All the stresses result in a series of physiological and metabolic reactions and then generate reversible inhibition of metabolism and growth and can cause seriously irreversible damage, even death. At each stage of cotton growth, environmental stress conditions pose devastating threats to plant growth and development, especially yield and quality. Due to the complex stress conditions and unclear molecular mechanisms of stress response, there is an urgent need to explore the mechanisms of cotton response against abiotic stresses. Methodology and Principal Findings A normalized cDNA library was constructed using Gossypium barbadense Hai-7124 treated with different stress conditions (heat, cold, salt, drought, potassium and phosphorus deficit and Verticillium dahliae infection). Random sequencing of this library generated 6,047 high-quality expressed sequence tags (ESTs). The ESTs were clustered and assembled into 3,135 uniESTs, composed of 2,497 contigs and 638 singletons. The blastx results demonstrated 2,746 unigenes showing significant similarity to known genes, 74 uniESTs displaying significant similarity to genes of predicted proteins, and 315 uniESTs remain uncharacterized. Functional classification unveiled the abundance of uniESTs in binding, catalytic activity, and structural molecule activity. Annotations of the uniESTs by the plant transcription factor database (PlantTFDB) and Plant Stress Protein Database (PSPDB) disclosed that transcription factors and stress-related genes were enriched in the current library. The expression of some transcription factors and specific stress-related genes were verified by RT-PCR under various stress conditions. Conclusions/Significance Annotation results showed that a huge number of genes respond to stress in our study, such as MYB-related, C2H2, FAR1, bHLH, bZIP, MADS, and mTERF. These results will improve our knowledge of stress tolerance in cotton. In addition, they are also helpful in discovering candidate genes related to stress tolerance. The publicly available ESTs from G. barbadense are a valuable genomic resource that will facilitate further molecular study and breeding of stress-tolerant cotton.
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an ethylene response related factor gberf1 like from Gossypium barbadense improves resistance to verticillium dahliae via activating lignin synthesis
Plant Molecular Biology, 2016Co-Authors: W W Guo, Longfu Zhu, Li Jin, Yuhuan Miao, Kai Guo, Xianlong ZhangAbstract:An ethylene response-related factor, GbERF1-like, from Gossypium barbadense cv. '7124' involved in the defence response to Verticillium dahliae was characterized. GbERF1-like transcripts present ubiquitously in various tissues, with higher accumulation in flower organs. GbERF1-like was also responsive to defence-related phytohormones and V. dahliae infection. The downregulation of GbERF1-like increased the susceptibility of cotton plants to V. dahliae infection, while overexpression of this gene improved disease resistance in both cotton and Arabidopsis, coupled with activation of the pathogenesis-related proteins. Further analysis revealed that genes involved in lignin synthesis, such as PAL, C4H, C3H, HCT, CCoAOMT, CCR and F5H, showed higher expression levels in the overexpressing cotton and Arabidopsis lines and lower expression levels in the RNAi cotton lines. The expression levels of these genes increased obviously when the GbERF1-like-overexpressing plants were inoculated with V. dahliae. Meanwhile, significant differences in the content of whole lignin could be found in the stems of transgenic and wild-type plants after inoculation with V. dahliae, as revealed by metabolic and histochemical analysis. More lignin could be detected in GbERF1-like-overexpressing cotton and Arabidopsis but less in GbERF1-like-silencing cotton compared with wild-type plants. The ratio of S and G monomers in GbERF1-like-overexpressing cotton and Arabidopsis increased significantly after infection by V. dahliae. Moreover, our results showed that the promoters of GhHCT1 and AtPAL3 could be transactivated by GbERF1-like in vivo based on yeast one-hybrid assays and dual-luciferase reporter assays. Knockdown of GhHCT1 in GbERF1-like over-expressing cotton decreases resistance to V. dahliae. Collectively, our results suggest that GbERF1-like acts as a positive regulator in lignin synthesis and contributes substantially to resistance to V. dahliae in plants.
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genetic diversity of sea island cotton Gossypium barbadense revealed by mapped ssrs
Genetics and Molecular Research, 2011Co-Authors: Xiaqing Wang, C H Feng, Zhongxu Lin, Xianlong ZhangAbstract:In order to evaluate the genetic diversity of sea- island cotton (Gossypium barbadense), 237 commonly mapped SSR markers covering the cotton genome were used to genotype 56 sea- island cotton accessions. A total of 218 polymorphic primer pairs (91.98%) amplified 361 loci, with a mean of 1.66 loci. Polymorphism information content values of the SSR primers ranged from 0.035 to 0.862, with a mean of 0.320. The highest mean polymorphism information content value for the SSR motifs was from a compound motif (0.402), and for the chromosomes it was Chr10 (0.589); the highest ratio of polymorphic primers in Xinjiang accessions was from Chr21 (83.33%). Genetic diversity was high in Xinjiang accessions. AMOVA showed that variation was 8 and 92% among populations and within populations, respectively. The 56 sea-island accessions were divided into three groups in the UPGMA dendrogram: Xinhai5 was in the first group; accessions from Xinjiang, except the five main ones, were in the second group, and the other 34 accessions
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genes expression analyses of sea island cotton Gossypium barbadense l during fiber development
Plant Cell Reports, 2007Co-Authors: Xianlong Zhang, Shaoguang Liang, Diqiu Liu, Longfu Zhu, Fanchang Zeng, Yichun Nie, Xiaoping Guo, Fenglin Deng, Jiafu TanAbstract:Sea-island cotton (Gossypium barbadense L.) is one of the most valuable cotton species due to its silkiness, luster, long staples, and high strength, but its fiber development mechanism has not been surveyed comprehensively. We constructed a normalized fiber cDNA library (from -2 to 25 dpa) of G. barbadense cv. Pima 3-79 (the genetic standard line) by saturation hybridization with genomic DNA. We screened Pima 3-79 fiber RNA from five developmental stages using a cDNA array including 9,126 plasmids randomly selected from the library, and we selected and sequenced 929 clones that had different signal intensities between any two stages. The 887 high-quality expressed sequence tags obtained were assembled into 645 consensus sequences (582 singletons and 63 contigs), of which 455 were assigned to functional categories using gene ontology. Almost 50% of binned genes belonged to metabolism functional categories. Based on subarray analysis of the 887 high-quality expressed sequence tags with 0-, 5-, 10-, 15-, and 20-dpa RNA of Pima 3-79 fibers and a mixture of RNA of nonfiber tissues, seven types of expression profiles were elucidated. Furthermore our results showed that phytohormones may play an important role in the fiber development.
Tianzhen Zhang - One of the best experts on this subject based on the ideXlab platform.
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cotton fiber elongation network revealed by expression profiling of longer fiber lines introgressed with different Gossypium barbadense chromosome segments
BMC Genomics, 2014Co-Authors: Lei Fang, Jiedan Chen, Ruiping Tian, Sen Wang, Peng Wang, Tianzhen ZhangAbstract:Cotton fiber, a highly elongated, thickened single cell of the seed epidermis, is a powerful cell wall research model. Fiber length, largely determined during the elongation stage, is a key property of fiber quality. Several studies using expressed sequence tags and microarray analysis have identified transcripts that accumulate preferentially during fiber elongation. To further show the mechanism of fiber elongation, we used Digital Gene Expression Tag Profiling to compare transcriptome data from longer fiber chromosome introgressed lines (CSILs) containing segments of various Gossypium barbadense chromosomes with data from its recurrent parent TM-1 during fiber elongation (from 5 DPA to 20 DPA). A large number of differentially expressed genes (DEGs) involved in carbohydrate, fatty acid and secondary metabolism, particularly cell wall biosynthesis, were highly upregulated during the fiber elongation stage, as determined by functional enrichment and pathway analysis. Furthermore, DEGs related to hormone responses and transcription factors showed upregulated expression levels in the CSILs. Moreover, metabolic and regulatory network analysis indicated that the same pathways were differentially altered, and distinct pathways exhibited altered gene expression, in the CSILs. Interestingly, mining of upregulated DEGs in the introgressed segments of these CSILs based on D-genome sequence data showed that these lines were enriched in glucuronosyltransferase, inositol-1, 4, 5-trisphosphate 3-kinase and desulfoglucosinolate sulfotransferase activity. These results were similar to the results of transcriptome analysis. This report provides an integrative network about the molecular mechanisms controlling fiber length, which are mainly tied to carbohydrate metabolism, cell wall biosynthesis, fatty acid metabolism, secondary metabolism, hormone responses and Transcription factors. The results of this study provide new insights into the critical factors associated with cell elongation and will facilitate further research aimed at understanding the mechanisms underlying cotton fiber elongation.
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characterization of expressed sequence tags from developing fibers of Gossypium barbadense and evaluation of insertion deletion variation in tetraploid cultivated cotton species
BMC Genomics, 2013Co-Authors: Yuanda Lv, Liang Zhao, Xiaoyang Xu, Lei Wang, Cheng Wang, Tianzhen ZhangAbstract:Cotton is the leading fiber crop worldwide. Gossypium barbadense is an important species of cotton because of its extra-long staple fibers with superior luster and silkiness. However, a systematic analysis and utilization of cDNA sequences from G. barbadense fiber development remains understudied. A total of 21,079 high quality sequences were generated from two non-normalized cDNA libraries prepared by using a mixture of G. barbadense Hai7124 fibers and ovules. After assembly processing, a set of 8,653 unigenes were obtained. Of those, 7,786 were matched to known proteins and 7,316 were assigned to functional categories. The molecular functions of these unigenes were mostly related to binding and catalytic activity, and carbohydrate, amino acid, and energy metabolisms were major contributors among the subsets of metabolism. Sequences comparison between G. barbadense and G. hirsutum revealed that 8,245 unigenes from G. barbadense were detected the similarity with those released publicly in G. hirsutum, however, the remaining 408 sequences had no hits against G. hirsutum unigenes database. Furthermore, 13,275 putative ESTs InDels loci involved in the orthologous and/or homoeologous differences between/within G. barbadense and G. hirsutum were discovered by in silico analyses, and 2,160 InDel markers were developed by ESTs with more than five insertions or deletions. By gel electrophoresis combined with sequencing verification, 71.11% candidate InDel loci were reconfirmed orthologous and/or homoeologous loci polymorphisms using G. hirsutum acc TM-1 and G. barbadense cv Hai7124. Blastx result showed among 2,160 InDel loci, 81 with significant function similarity with known genes associated with secondary wall synthesis process, indicating the important roles in fiber quality in tetraploid cultivated cotton species. Sequence comparisons and InDel markers development will lay the groundwork for promoting the identification of genes related to superior agronomic traits, genetic differentiation and comparative genomic studies between G. hirsutum and G. barbadense.
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qtls mapping for verticillium wilt resistance at seedling and maturity stages in Gossypium barbadense l
Plant Science, 2008Co-Authors: Chang Yang, Wangzhen Guo, Feng Gao, Shunshun Lin, Tianzhen ZhangAbstract:Abstract Verticillium wilt ( Verticillium dahliae ) is a major concern for cotton producers and consumers. The major strategy to control disease has been the development of resistant varieties of Gossypium . To identify Verticilium resistant genes in cotton, we generated F 2 and BC 1 populations by crossing a tolerant cultivar of Gossypium barbadense var. Hai 7124 with a susceptible cultivar, Gossypium hirsutum var. Junmian 1. Individual plant reaction to disease was quantified using a leaf trait in the seedling stage and a vascular tissue trait in mature stage. Two genetic linkage maps were constructed with SSR, RGA and DDRT markers. Four QTLs for the leaf trait, located on chr. A5, A7 and A8 and three QTLs for the vascular tissue trait on chr. A5, A7 and A9 were detected by composite interval mapping (CIM) in F 2 inoculated with a non-defoliating isolate BP2. BC 1 S 2 families were planted separately and inoculated with the isolates, BP2, VD8 and 592. In the BP2 nursery, one QTL located on chr. D4 for the leaf trait and two QTLs on chr. D4 and A8 for the vascular tissue trait were detected. We detected two QTLs on chr. A5 and A8 for the leaf trait and three QTLs on chr. A5, D5 and D11 for the vascular tissue trait in plants grown in the VD8 nursery. In the 592 nursery, three QTLs on chr. A5 and D5 for the leaf trait and two QTLs on chr. D5 and D11 for the vascular tissue trait were detected. The present QTL mapping results revealed that two stable QTLs existed in different populations, four QTLs expressed at both the seedling and mature stages, and some QTLs controlling resistance to Verticillium wilt were located on different chromosomes and in different developmental stages for different isolates. The markers associated with these QTLs may facilitate the use of Verticillium wilt resistant genes in resistant breeding programs for cotton.
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a comparison of genetic maps constructed from haploid and bc1 mapping populations from the same crossing between Gossypium hirsutum l and Gossypium barbadense l
Genome, 2005Co-Authors: Xianliang Song, Wangzhen Guo, Kai Wang, Jun Zhang, Tianzhen ZhangAbstract:Simple sequence repeat (SSR) genetic maps have been separately constructed based on doubled haploid (DH) and (or) haploid and BC1 populations from the same cross between Gossypium hirsutum L. 'TM-1' and Gossypium barbadense L. 'Hai7124'. The BC1 population was produced by pollinating individual plants of the 'TM-1' × 'Hai7124' F1 with 'TM-1', whereas the DH and (or) haploid population developed from the offspring of Vsg × ('TM-1' × 'Hai7124'). Vsg is a virescently marked semigamy line of Gossypium barbadense L. Pima. The BC1 map included 34 linkage groups with an average distance between markers of 9.80 cM (Kosambi, K) and covered 4331.2 cM (K) or approximately 78.7% of the tetraploid cotton genome constructed using 440 SSR and 2 morphological marker genes. Among them, 26 were assigned to 20 chromosomes, 7 to A or D subgenomes, and 1 was unassigned. The haploid map comprised 444 SSR markers mapped to 40 linkage groups with an average distance of 7.35 cM (K) between markers, covering 3262.9 cM (K) or appro...
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molecular linkage map of allotetraploid cotton Gossypium hirsutum l Gossypium barbadense l with a haploid population
Theoretical and Applied Genetics, 2002Co-Authors: Jun Zhang, Wanlin Guo, Tianzhen ZhangAbstract:In the present study, a haploid population from the cross of the two cultivated allotetraploid cottons, Gossypium hirsutum L. and Gossypium barbadense L., was developed by means of Vsg, a virescently marked semigamous line of Sea island cotton, and some target haploids were successfully doubled with colchicine. A molecular linkage map was constructed with 58 doubled and haploid plants. Among the total of 624 marker loci (510 SSRs and 114 RAPDs), 489 loci were assembled into 43 linkage groups and covered 3,314.5 centi-Morgans (cM). Using the monosomic and telodisomic genetic stocks, the linkage groups of the present map were associated with chromosomes of the allotetraploid genome, and some of the unassociated groups were connected to corresponding A or D subgenomes. Through the analysis of the assignment of the duplicated SSR loci in the chromosomes or the linkage groups, ten pairs of possible homoeologous chromosome (or linkage group) regions were identified. Among them, the pairs of Chrs. 1 and 15, Chrs. 4 and 22, and Chrs. 10 and 20 had already been determined as homoeologous by classical genetic and cytogenetic research, and the pair of Chrs. 9 and 23 had also been identified by the ISH method of molecular cytogenetics. But, from present research, it was assumed that Chrs. 5 and 18 might be a new pair of homoeologous chromosomes of the allotetraploid cotton genome detected by molecular mapping of the cotton genome.
Lorraine S Puckhaber - One of the best experts on this subject based on the ideXlab platform.
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differences in active defense responses of two Gossypium barbadense l cultivars resistant to fusarium oxysporum f sp vasinfectum race 4
Journal of Agricultural and Food Chemistry, 2018Co-Authors: Lorraine S Puckhaber, Robert D Stipanovic, Xiuting Zheng, Alois A Bell, Robert L Nichols, Jinggao Liu, Sara E DukeAbstract:A highly virulent race 4 genotype of Fusarium oxysporum f. sp. vasinfectum (Fov) was identified for the first time in the western hemisphere in 2002 in cotton fields in the San Joaquin Valley of California. The Gossypium barbadense L. cotton cultivars ‘Seabrook Sea Island 12B2’ (‘SBSI’) and ‘Pima S-6’ are resistant to Fov race 4. Active defense responses were quantitated by monitoring the accumulation of antimicrobial terpenoids (i.e., phytoalexins) in inoculated stem stele tissue in these cultivars. The increase in the concentration of the most toxic phytoalexins was statistically faster after 24 h in ‘SBSI’ compared to ‘Pima S-6’. The sesquiterpenoid hemigossylic acid lactone, which was observed for the first time in nature, also accumulated in diseased plants. Neither hemigossylic acid lactone nor the disesquiterpenoids gossypol, gossypol-6-methyl ether, and gossypol-6,6′-dimethyl ether showed toxicity to Fov. Segregation of F2 progeny from ‘SBSI’ × ‘Pima S-6’ crosses gave a few highly susceptible plan...
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Differences in Active Defense Responses of Two Gossypium barbadense L. Cultivars Resistant to Fusarium oxysporum f. sp. vasinfectum Race 4
2018Co-Authors: Lorraine S Puckhaber, Robert D Stipanovic, Xiuting Zheng, Alois A Bell, Robert L Nichols, Jinggao Liu, Sara E DukeAbstract:A highly virulent race 4 genotype of Fusarium oxysporum f. sp. vasinfectum (Fov) was identified for the first time in the western hemisphere in 2002 in cotton fields in the San Joaquin Valley of California. The Gossypium barbadense L. cotton cultivars ‘Seabrook Sea Island 12B2’ (‘SBSI’) and ‘Pima S-6’ are resistant to Fov race 4. Active defense responses were quantitated by monitoring the accumulation of antimicrobial terpenoids (i.e., phytoalexins) in inoculated stem stele tissue in these cultivars. The increase in the concentration of the most toxic phytoalexins was statistically faster after 24 h in ‘SBSI’ compared to ‘Pima S-6’. The sesquiterpenoid hemigossylic acid lactone, which was observed for the first time in nature, also accumulated in diseased plants. Neither hemigossylic acid lactone nor the disesquiterpenoids gossypol, gossypol-6-methyl ether, and gossypol-6,6′-dimethyl ether showed toxicity to Fov. Segregation of F2 progeny from ‘SBSI’ × ‘Pima S-6’ crosses gave a few highly susceptible plants and a few highly resistant plants, indicating separate genes for resistance in the two cultivars
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total and percent atropisomers of gossypol and gossypol 6 methyl ether in seeds from pima cottons and accessions of Gossypium barbadense l
Journal of Agricultural and Food Chemistry, 2009Co-Authors: Robert D Stipanovic, Lorraine S Puckhaber, Jinggao Liu, Alois A BellAbstract:Gossypol occurs naturally in the seed, foliage, and roots of the cotton plant ( Gossypium ) as atropisomers due to restricted rotation around the binaphthyl bond. The atropisomers differ in their biological activities. (-)-(R)-Gossypol is more toxic and exhibits significantly greater anticancer activity than the (+)-(S)-atropisomer. Most commercial Upland ( Gossypium hirsutum ) cottonseeds have an (R)- to (S)-gossypol ratio of approximately 2:3, but some Pima ( Gossypium barbadense ) seeds have an excess of (R)-gossypol. There is no known source of cottonseed with an (R)- to (S)-gossypol ratio of greater than approximately 70:30. Cottonseed with a high percentage of (R)-gossypol would be of value to the pharmaceutical industry. It was theorized that G. barbadense cotton might be a source of this desirable high (R)-gossypol seed trait. There are 671 different accessions of G. barbadense in the U.S. Cotton Germplasm Collection, few of which had been characterized with respect to their (R)- to (S)-gossypol ratio. This work completed that analysis and found considerable variation in the atropisomer ratio. Approximately half of the accessions have an excess of (R)-gossypol, and 52 accessions have essentially a 1:1 ratio. The highest percentage of (R)-gossypol was found in accessions GB26 (68.2%) and GB283 (67.3%). Surprisingly, five accessions had 5% or less of (R)-gossypol: GB516 (5.0%), GB761 (4.5%), GB577 (4.3%), GB719 (3.7%), and GB476 (2.3%). These accessions may be useful in a breeding program to reduce (R)-gossypol in Pima seed, which is a concern to the dairy industry because of the toxicity and male antifertility activity of this atropisomer. Also, GB710 was devoid of gossypol.
Sara E Duke - One of the best experts on this subject based on the ideXlab platform.
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differences in active defense responses of two Gossypium barbadense l cultivars resistant to fusarium oxysporum f sp vasinfectum race 4
Journal of Agricultural and Food Chemistry, 2018Co-Authors: Lorraine S Puckhaber, Robert D Stipanovic, Xiuting Zheng, Alois A Bell, Robert L Nichols, Jinggao Liu, Sara E DukeAbstract:A highly virulent race 4 genotype of Fusarium oxysporum f. sp. vasinfectum (Fov) was identified for the first time in the western hemisphere in 2002 in cotton fields in the San Joaquin Valley of California. The Gossypium barbadense L. cotton cultivars ‘Seabrook Sea Island 12B2’ (‘SBSI’) and ‘Pima S-6’ are resistant to Fov race 4. Active defense responses were quantitated by monitoring the accumulation of antimicrobial terpenoids (i.e., phytoalexins) in inoculated stem stele tissue in these cultivars. The increase in the concentration of the most toxic phytoalexins was statistically faster after 24 h in ‘SBSI’ compared to ‘Pima S-6’. The sesquiterpenoid hemigossylic acid lactone, which was observed for the first time in nature, also accumulated in diseased plants. Neither hemigossylic acid lactone nor the disesquiterpenoids gossypol, gossypol-6-methyl ether, and gossypol-6,6′-dimethyl ether showed toxicity to Fov. Segregation of F2 progeny from ‘SBSI’ × ‘Pima S-6’ crosses gave a few highly susceptible plan...
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Differences in Active Defense Responses of Two Gossypium barbadense L. Cultivars Resistant to Fusarium oxysporum f. sp. vasinfectum Race 4
2018Co-Authors: Lorraine S Puckhaber, Robert D Stipanovic, Xiuting Zheng, Alois A Bell, Robert L Nichols, Jinggao Liu, Sara E DukeAbstract:A highly virulent race 4 genotype of Fusarium oxysporum f. sp. vasinfectum (Fov) was identified for the first time in the western hemisphere in 2002 in cotton fields in the San Joaquin Valley of California. The Gossypium barbadense L. cotton cultivars ‘Seabrook Sea Island 12B2’ (‘SBSI’) and ‘Pima S-6’ are resistant to Fov race 4. Active defense responses were quantitated by monitoring the accumulation of antimicrobial terpenoids (i.e., phytoalexins) in inoculated stem stele tissue in these cultivars. The increase in the concentration of the most toxic phytoalexins was statistically faster after 24 h in ‘SBSI’ compared to ‘Pima S-6’. The sesquiterpenoid hemigossylic acid lactone, which was observed for the first time in nature, also accumulated in diseased plants. Neither hemigossylic acid lactone nor the disesquiterpenoids gossypol, gossypol-6-methyl ether, and gossypol-6,6′-dimethyl ether showed toxicity to Fov. Segregation of F2 progeny from ‘SBSI’ × ‘Pima S-6’ crosses gave a few highly susceptible plants and a few highly resistant plants, indicating separate genes for resistance in the two cultivars
Robert L Nichols - One of the best experts on this subject based on the ideXlab platform.
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differences in active defense responses of two Gossypium barbadense l cultivars resistant to fusarium oxysporum f sp vasinfectum race 4
Journal of Agricultural and Food Chemistry, 2018Co-Authors: Lorraine S Puckhaber, Robert D Stipanovic, Xiuting Zheng, Alois A Bell, Robert L Nichols, Jinggao Liu, Sara E DukeAbstract:A highly virulent race 4 genotype of Fusarium oxysporum f. sp. vasinfectum (Fov) was identified for the first time in the western hemisphere in 2002 in cotton fields in the San Joaquin Valley of California. The Gossypium barbadense L. cotton cultivars ‘Seabrook Sea Island 12B2’ (‘SBSI’) and ‘Pima S-6’ are resistant to Fov race 4. Active defense responses were quantitated by monitoring the accumulation of antimicrobial terpenoids (i.e., phytoalexins) in inoculated stem stele tissue in these cultivars. The increase in the concentration of the most toxic phytoalexins was statistically faster after 24 h in ‘SBSI’ compared to ‘Pima S-6’. The sesquiterpenoid hemigossylic acid lactone, which was observed for the first time in nature, also accumulated in diseased plants. Neither hemigossylic acid lactone nor the disesquiterpenoids gossypol, gossypol-6-methyl ether, and gossypol-6,6′-dimethyl ether showed toxicity to Fov. Segregation of F2 progeny from ‘SBSI’ × ‘Pima S-6’ crosses gave a few highly susceptible plan...
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Differences in Active Defense Responses of Two Gossypium barbadense L. Cultivars Resistant to Fusarium oxysporum f. sp. vasinfectum Race 4
2018Co-Authors: Lorraine S Puckhaber, Robert D Stipanovic, Xiuting Zheng, Alois A Bell, Robert L Nichols, Jinggao Liu, Sara E DukeAbstract:A highly virulent race 4 genotype of Fusarium oxysporum f. sp. vasinfectum (Fov) was identified for the first time in the western hemisphere in 2002 in cotton fields in the San Joaquin Valley of California. The Gossypium barbadense L. cotton cultivars ‘Seabrook Sea Island 12B2’ (‘SBSI’) and ‘Pima S-6’ are resistant to Fov race 4. Active defense responses were quantitated by monitoring the accumulation of antimicrobial terpenoids (i.e., phytoalexins) in inoculated stem stele tissue in these cultivars. The increase in the concentration of the most toxic phytoalexins was statistically faster after 24 h in ‘SBSI’ compared to ‘Pima S-6’. The sesquiterpenoid hemigossylic acid lactone, which was observed for the first time in nature, also accumulated in diseased plants. Neither hemigossylic acid lactone nor the disesquiterpenoids gossypol, gossypol-6-methyl ether, and gossypol-6,6′-dimethyl ether showed toxicity to Fov. Segregation of F2 progeny from ‘SBSI’ × ‘Pima S-6’ crosses gave a few highly susceptible plants and a few highly resistant plants, indicating separate genes for resistance in the two cultivars
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Identification of QTL regions and SSR markers associated with resistance to reniform nematode in Gossypium barbadense L. accession GB713
Theoretical and Applied Genetics, 2011Co-Authors: Osman A. Gutiérrez, Johnie N. Jenkins, Jack C. Mccarty, Arin F. Robinson, Martin J. Wubben, Franklin E. Callahan, Robert L NicholsAbstract:The identification of molecular markers that are closely linked to gene(s) in Gossypium barbadense L. accession GB713 that confer a high level of resistance to reniform nematode (RN), Rotylenchulus reniformis Linford & Oliveira, would be very useful in cotton breeding programs. Our objectives were to determine the inheritance of RN resistance in the accession GB713, to identify SSR markers linked with RN resistance QTLs, and to map these linked markers to specific chromosomes. We grew and scored plants for RN reproduction in the P_1, P_2, F_1, F_2, BC_1P_1, and BC_1P_2 generations from the cross of GB713 × Acala Nem-X. The generation means analysis using the six generations indicated that one or more genes were involved in the RN resistance of GB713. The interspecific F_2 population of 300 plants was genotyped with SSR molecular markers that covered most of the chromosomes of Upland cotton ( G. hirsutum L.). Results showed two QTLs on chromosome 21 and one QTL on chromosome 18. One QTL on chromosome 21 was at map position 168.6 (LOD 28.0) flanked by SSR markers, BNL 1551_162 and GH 132_199 at positions 154.2 and 177.3, respectively. A second QTL on chromosome 21 was at map position 182.7 (LOD 24.6) flanked by SSR markers BNL 4011_155 and BNL 3279_106 at positions 180.6 and 184.5, respectively. Our chromosome 21 map had 61 SSR markers covering 219 cM. One QTL with smaller genetic effects was localized to chromosome 18 at map position 39.6 (LOD 4.0) and flanked by SSR markers BNL 1721_178 and BNL 569_131 at positions 27.6 and 42.9, respectively. The two QTLs on chromosome 21 had significant additive and dominance effects, which were about equal for each QTL. The QTL on chromosome 18 showed larger additive than dominance effects. Following the precedent set by the naming of the G. longicalyx Hutchinson & Lee and G. aridum [(Rose & Standley) Skovsted] sources of resistance, we suggest the usage of Ren ^ barb1 and Ren ^ barb2 to designate these QTLs on chromosome 21 and Ren ^ barb3 on chromosome 18.
