The Experts below are selected from a list of 279 Experts worldwide ranked by ideXlab platform
M. Hafner - One of the best experts on this subject based on the ideXlab platform.
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Rapid fluorometric test for the quantitative determination of deoxynivalenol in raw cereals
Mycotoxin Research, 2007Co-Authors: M. Hafner, Z. Kubus, M. Freudenschuss, E. M. Binder, R. KrskaAbstract:Fast test systems fulfilling legislative specifications are required to determine mycotoxin contamination in unprocessed cereals e.g. at Grain Elevators, import and export terminals, or the milling and brewing industry to prevent contamination of food and feed. We describe the first tests of a novel fluorescence-based test for deoxynivalenol (DON), which will be commercially available within the next few months. The analytical procedure of the new test takes less than 15 minutes for extraction, purification, derivatization and measurement. The system’s advantage is its speed and easy procedure providing quantitative DON determination. To ensure an international standard, the validation procedure for wheat, barley and maize will be performed following USDA/GIPSA requirements (03/2006) for DON tests.
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Rapid fluorometric test for the quantitative determination of deoxynivalenol in raw cereals.
Mycotoxin research, 2007Co-Authors: M. Hafner, Z. Kubus, M. Freudenschuss, E. M. Binder, Rudolf KrskaAbstract:Fast test systems fulfilling legislative specifications are required to determine mycotoxin contamination in unprocessed cerealse.g. at Grain Elevators, import and export terminals, or the milling and brewing industry to prevent contamination of food and feed.
Rudolf Krska - One of the best experts on this subject based on the ideXlab platform.
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Mycotoxins and other fungal metabolites in Grain dust from Norwegian Grain Elevators and compound feed mills
World Mycotoxin Journal, 2015Co-Authors: Anne Straumfors, Kari Kulvik Heldal, Wijnand Eduard, S. Uhlig, Gunnar Sundstøl Eriksen, Rudolf Krska, Michael SulyokAbstract:Employees at Grain Elevators and compound feed mills are exposed to large amounts of Grain dust during work, frequently leading to airway symptoms and asthma. Although the exposure to Grain dust, microorganisms, β-1→3-glucans and endotoxins has been extensively studied, the focus on the mycotoxin content of Grain dust has previously been limited to one or few mycotoxins. Our objective was therefore to screen settled Grain dust from Grain Elevators and compound feed mills for fungal metabolites by LC/MS-MS and explore differences between work places, seasons and climatic zones. Seventy fungal metabolites and two bacterial metabolites were detected. Trichothecenes, depsipeptides, ergot alkaloids, and other metabolites from Fusarium, Claviceps, Alternaria, Penicillium, Aspergillus, and other fungi were represented. The prevalence of individual metabolites was highly variable, and the concentration of each metabolite varied considerably between samples. The prevalence and concentration of most metabolites wer...
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Rapid fluorometric test for the quantitative determination of deoxynivalenol in raw cereals.
Mycotoxin research, 2007Co-Authors: M. Hafner, Z. Kubus, M. Freudenschuss, E. M. Binder, Rudolf KrskaAbstract:Fast test systems fulfilling legislative specifications are required to determine mycotoxin contamination in unprocessed cerealse.g. at Grain Elevators, import and export terminals, or the milling and brewing industry to prevent contamination of food and feed.
Wijnand Eduard - One of the best experts on this subject based on the ideXlab platform.
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on behalf of the British Occupational Hygiene Society. Exposure to Grain Dust and Microbial Components in the Norwegian Grain and Compound Feed Industry
2016Co-Authors: Ann Occup Hyg, Kari Kulvik Heldal, Inge M Wouters, Anne Straumfors Halstensen, Marit Skogstad, Dag G Ellingsen, Wijnand EduardAbstract:Objectives: The aim of this study was to extensively characterize Grain workers ’ personal expo-sure during work in Norwegian Grain Elevators and compound feed mills, to identify differences in exposures between the workplaces and seasons, and to study the correlations between different microbial components. Methods: Samples of airborne dust (n = 166) were collected by full-shift personal sampling during work in 20 Grain Elevators and compound feed mills during one autumn season and two winter seasons. The personal exposure to Grain dust, endotoxins, β-1→3-glucans, bacteria, and fungal spores was quantified. Correlations between dust and microbial components and differences between workplaces and seasons were investigated. Determinants of endotoxin and β-1→3-glucan exposure were evaluated by linear mixed-effect regression modeling. Results: The workers were exposed to an overall geometric mean of 1.0 mg m−3 inhalable Grain dust [geometric standard deviation (GSD) = 3.7], 628 endotoxin units m−3 (GSD = 5.9), 7.4 µg m−3 of β-1→3-glucan (GSD = 5.6), 21 × 104 bacteria m−3 (GSD = 7.9) and 3.6 × 104 fungal spores m−3 (GSD = 3.4). The Grain dust exposure levels were similar across workplaces and seasons, but the microbial content of the Grain dust varied substantially between workplaces. Exposure level
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Work Tasks as Determinants of Grain Dust and Microbial Exposure in the Norwegian Grain and Compound Feed Industry
The Annals of occupational hygiene, 2015Co-Authors: Anne Straumfors, Kari Kulvik Heldal, Inge M Wouters, Wijnand EduardAbstract:The Grain and compound feed industry entails inevitable risks of exposure to Grain dust and its microbial content. The objective of this study was therefore to investigate task-dependent exposure differences in order to create knowledge basis for awareness and exposure reducing measures in the Norwegian Grain and compound feed industry. A total of 166 samples of airborne dust were collected by full-shift personal sampling during work in 20 Grain Elevators and compound feed mills during one autumn season and two winter seasons. The personal exposure to Grain dust, endotoxins, β-1→3-glucans, bacteria, and fungal spores was quantified and used as individual outcomes in mixed models with worker nested in company as random effect and different departments and tasks as fixed effects. The exposure levels were highest in Grain elevator departments. Exposure to endotoxins was particularly high. Tasks that represented the highest and lowest exposures varied depending on the bioaerosol component. The most important determinants for elevated dust exposure were cleaning and process controlling. Cleaning increased the dust exposure level by a factor of 2.44 of the reference, from 0.65 to 1.58mg m(-3), whereas process controlling increased the dust exposure level by a factor of 2.97, from 0.65 to 1.93mg m(-3). Process controlling was associated with significantly less Grain dust exposure in compound feed mills and the combined Grain Elevators and compound feed mills, than in Grain Elevators. The exposure was reduced by a factor of 0.18 and 0.22, from 1.93 to 0.34mg m(-3) and to 0.42mg m(-3), respectively, compared with the Grain Elevators. Inspection/maintenance, cleaning, and Grain rotation and emptying were determinants of higher exposure to both endotoxin and β-1→3-glucans. Seed winnowing was in addition a strong determinant for endotoxin, whereas mixing of animal feed implied higher β-1→3-glucan exposure. Cleaning was the only task that contributed significantly to higher exposure to bacteria and fungal spores. Cleaning in all companies and process controlling in Grain Elevators were the strongest determinants for overall exposure, whereas seed winnowing was a particular strong determinant of endotoxin exposure. Exposure reduction by technical intervention or personal protective equipment should therefore be considered at work places with identified high exposure tasks. © The Author 2015. Published by Oxford University Press on behalf of the British Occupational Hygiene Society.
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Mycotoxins and other fungal metabolites in Grain dust from Norwegian Grain Elevators and compound feed mills
World Mycotoxin Journal, 2015Co-Authors: Anne Straumfors, Kari Kulvik Heldal, Wijnand Eduard, S. Uhlig, Gunnar Sundstøl Eriksen, Rudolf Krska, Michael SulyokAbstract:Employees at Grain Elevators and compound feed mills are exposed to large amounts of Grain dust during work, frequently leading to airway symptoms and asthma. Although the exposure to Grain dust, microorganisms, β-1→3-glucans and endotoxins has been extensively studied, the focus on the mycotoxin content of Grain dust has previously been limited to one or few mycotoxins. Our objective was therefore to screen settled Grain dust from Grain Elevators and compound feed mills for fungal metabolites by LC/MS-MS and explore differences between work places, seasons and climatic zones. Seventy fungal metabolites and two bacterial metabolites were detected. Trichothecenes, depsipeptides, ergot alkaloids, and other metabolites from Fusarium, Claviceps, Alternaria, Penicillium, Aspergillus, and other fungi were represented. The prevalence of individual metabolites was highly variable, and the concentration of each metabolite varied considerably between samples. The prevalence and concentration of most metabolites wer...
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exposure to Grain dust and microbial components in the norwegian Grain and compound feed industry
Annals of Occupational Hygiene, 2013Co-Authors: Anne Straumfors Halstensen, Kari Kulvik Heldal, Inge M Wouters, Marit Skogstad, Dag G Ellingsen, Wijnand EduardAbstract:Objectives: The aim of this study was to extensively characterize Grain workers’ personal exposure during work in Norwegian Grain Elevators and compound feed mills, to identify differences in exposures between the workplaces and seasons, and to study the correlations between different microbial components. Methods: Samples of airborne dust (n = 166) were collected by full-shift personal sampling during work in 20 Grain Elevators and compound feed mills during one autumn season and two winter seasons. The personal exposure to Grain dust, endotoxins, β-1→3-glucans, bacteria, and fungal spores was quantified. Correlations between dust and microbial components and differences between workplaces and seasons were investigated. Determinants of endotoxin and β-1→3-glucan exposure were evaluated by linear mixed-effect regression modeling. Results: The workers were exposed to an overall geometric mean of 1.0 mg m −3 inhalable Grain dust [geometric standard deviation (GSD) = 3.7], 628 endotoxin units m −3 (GSD = 5.9), 7.4 µg m −3 of β-1→3-glucan (GSD = 5.6), 21 × 10 4 bacteria m −3 (GSD = 7.9) and 3.6 × 10 4 fungal spores m −3 (GSD = 3.4). The Grain dust exposure levels were similar across workplaces and seasons, but the microbial content of the Grain dust varied substantially between workplaces. Exposure levels of all microbial components were significantly higher in Grain Elevators compared with all other workplaces. The Grain dust exposure was significantly correlated (Pearson’s r) with endotoxin (r p = 0.65), β-1→3-glucan (r p = 0.72), bacteria (r p = 0.44) and fungal spore (r p = 0.48) exposure, whereas the explained variances were strongly dependent on the workplace. Bacteria, Grain dust, and workplace were important determinants for endotoxin exposure, whereas fungal spores, Grain dust, and workplace were important determinants for β-1→3-glucan exposure. Conclusions: Although the workers were exposed to a relatively low mean dust level, the microbial exposure was high. Furthermore, the exposure levels of microbial components varied between workplaces although the dust levels were similar. We therefore recommend that exposure levels at different workplaces should be assessed separately and a task-based assessment should be done for detailed evaluation of efficient dust-reducing measures. The microbial content and knowledge of health effects of the microbial components should be considered in health risk evaluations of these workplaces.
R. Krska - One of the best experts on this subject based on the ideXlab platform.
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Rapid fluorometric test for the quantitative determination of deoxynivalenol in raw cereals
Mycotoxin Research, 2007Co-Authors: M. Hafner, Z. Kubus, M. Freudenschuss, E. M. Binder, R. KrskaAbstract:Fast test systems fulfilling legislative specifications are required to determine mycotoxin contamination in unprocessed cereals e.g. at Grain Elevators, import and export terminals, or the milling and brewing industry to prevent contamination of food and feed. We describe the first tests of a novel fluorescence-based test for deoxynivalenol (DON), which will be commercially available within the next few months. The analytical procedure of the new test takes less than 15 minutes for extraction, purification, derivatization and measurement. The system’s advantage is its speed and easy procedure providing quantitative DON determination. To ensure an international standard, the validation procedure for wheat, barley and maize will be performed following USDA/GIPSA requirements (03/2006) for DON tests.
Z. Kubus - One of the best experts on this subject based on the ideXlab platform.
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Rapid fluorometric test for the quantitative determination of deoxynivalenol in raw cereals
Mycotoxin Research, 2007Co-Authors: M. Hafner, Z. Kubus, M. Freudenschuss, E. M. Binder, R. KrskaAbstract:Fast test systems fulfilling legislative specifications are required to determine mycotoxin contamination in unprocessed cereals e.g. at Grain Elevators, import and export terminals, or the milling and brewing industry to prevent contamination of food and feed. We describe the first tests of a novel fluorescence-based test for deoxynivalenol (DON), which will be commercially available within the next few months. The analytical procedure of the new test takes less than 15 minutes for extraction, purification, derivatization and measurement. The system’s advantage is its speed and easy procedure providing quantitative DON determination. To ensure an international standard, the validation procedure for wheat, barley and maize will be performed following USDA/GIPSA requirements (03/2006) for DON tests.
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Rapid fluorometric test for the quantitative determination of deoxynivalenol in raw cereals.
Mycotoxin research, 2007Co-Authors: M. Hafner, Z. Kubus, M. Freudenschuss, E. M. Binder, Rudolf KrskaAbstract:Fast test systems fulfilling legislative specifications are required to determine mycotoxin contamination in unprocessed cerealse.g. at Grain Elevators, import and export terminals, or the milling and brewing industry to prevent contamination of food and feed.
