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Pamela C Ronald - One of the best experts on this subject based on the ideXlab platform.
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tyrosine sulfation in a Gram Negative Bacterium
Nature Communications, 2012Co-Authors: Ofir Bahar, Benjamin Schwessinger, Michelle R Robinson, Jared B Shaw, James A Madsen, Jennifer S Brodbelt, Pamela C RonaldAbstract:The sulfation of protein tyrosine residues is a common post-translational modification in eukaryotes. Here, Han et al. show that the protein RaxST, produced by a plant Bacterium, has tyrosine sulfotransferase activity, demonstrating for the first time tyrosine sulfation in prokaryotes.
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Tyrosine sulfation in a Gram-Negative Bacterium
Nature communications, 2012Co-Authors: Sang-wook Han, Ofir Bahar, Benjamin Schwessinger, Michelle R Robinson, Jared B Shaw, James A Madsen, Jennifer S Brodbelt, Sang Won Lee, Pamela C RonaldAbstract:Tyrosine sulfation, a well-characterized post-translation modification in eukaryotes, has not previously been reported in prokaryotes. Here, we demonstrate that the RaxST protein from the Gram-Negative Bacterium, Xanthomonas oryzae pv. oryzae, is a tyrosine sulfotransferase. We used a newly developed sulfotransferase assay and ultraviolet photodissociation mass spectrometry to demonstrate that RaxST catalyses sulfation of tyrosine 22 of the Xoo Ax21 (activator of XA21-mediated immunity) protein. These results demonstrate a previously undescribed post-translational modification in a prokaryotic species with implications for studies of host immune responses and bacterial cell-cell communication systems.
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Small Protein-Mediated Quorum Sensing in a Gram-Negative Bacterium
PloS one, 2011Co-Authors: Sang-wook Han, Ofir Bahar, Sang Won Lee, Malinee Sriariyanun, Manoj K. Sharma, Zachary Bower, Pamela C RonaldAbstract:The rice XA21 pattern recognition receptor binds a type I secreted sulfated peptide, called axYS22, derived from the Ax21 (activator of XA21-mediated immunity) protein. The conservation of Ax21 in all sequenced Xanthomonas spp. and closely related genera suggests that Ax21 serves a key biological function. Here we show that the predicted N-terminal sequence of Ax21 is cleaved prior to secretion outside the cell and that mature Ax21 serves as a quorum sensing (QS) factor in Xanthomonas oryzae pv. oryzae. Ax21-mediated QS controls motility, biofilm formation and virulence. We provide genetic evidence that the Xoo RaxH histidine kinase serves as the bacterial receptor for Ax21. This work establishes a critical role for small protein-mediated QS in a Gram-Negative Bacterium.
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Small protein-mediated quorum sensing in a Gram-Negative Bacterium: novel targets for control of infectious disease.
Discovery medicine, 2011Co-Authors: Pamela C RonaldAbstract:Control of Gram-Negative bacterial infections of plants and animals remains a major challenge because conventional approaches are often not sufficient to eradicate these infections. One major reason for their persistence seems to be the capability of the bacteria to grow within biofilms that protect them from adverse environmental factors. Quorum sensing (QS) plays an important role in the formation of biofilms. In QS, small molecules serve as signals to recognize bacterial cell population size, leading to changes in expression of specific genes when a signal has accumulated to some threshold concentration. The small protein Ax21 (Activator of XA21-mediated immunity), serves as a QS factor that regulates biofilm formation and virulence in the Gram-Negative Bacterium, Xanthomonas oryzae pv. oryzae. Knowledge of small protein-mediated QS in Gram-Negative bacteria can be used to develop new methods to control persistent Gram-Negative infections.
Cheng-lin Jiang - One of the best experts on this subject based on the ideXlab platform.
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Rubellimicrobium roseum sp. nov., a Gram-Negative Bacterium isolated from the forest soil sample
Antonie van Leeuwenhoek, 2010Co-Authors: Yan-ru Cao, Yi Jiang, Qian Wang, Shu-kun Tang, Xue Quanhong, Cheng-lin JiangAbstract:A novel pink-coloured, non-spore-forming, non-motile, Gram-Negative Bacterium, designated YIM 48858T, is described by using a polyphasic approach. The strain can grow at pH 6.5–9 (optimum at pH 7) and 25–30°C (optimum at 28°C). NaCl is not required for its growth. Positive for oxidase and catalase. Urease activity, nitrate reduction, starch and Tween 80 tests are Negative reaction. 16S rRNA gene sequence similarity studies showed that strain YIM 48858T is a member of the genus Rubellimicrobium, with similarities of 96.3, 95.7 and 95.5% to Rubellimicrobium mesophilum MSL-20T, Rubellimicrobium aerolatum 5715S-9T and Rubellimicrobium thermophilum DSM 16684T, respectively. Q-10 was the predominant respiratory ubiquinone as in the other members of the genus Rubellimicrobium. The major polar lipids were diphosphatidylglycerol, phosphatidylcholine, phosphoglycolipid, glycolipid and the major fatty acids were C18:1 ω7c, C16:0 and C10:0 3-OH, which are very different from the valid published species. The DNA G + C content was 67.7 mol%. Both phylogenetic and chemotaxonomic evidence supports that YIM 48858T is a novel species of the genus Rubellimicrobium, for which the name Rubellimicrobium roseum sp. nov. is proposed. The type strain is YIM 48858T (=CCTCC AA 208029T =KCTC 23202T).
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Sphingomonas yunnanensis sp. nov., a novel Gram-Negative Bacterium from a contaminated plate
International Journal of Systematic and Evolutionary Microbiology, 2005Co-Authors: Yu-qin Zhang, Yi-guang Chen, Xin-peng Tian, Cheng-lin JiangAbstract:A Gram-Negative Bacterium, YIM 003T, which was isolated from a contaminated plate in the laboratory, was subjected to a polyphasic taxonomic study. The organism had short-rod-shaped, motile cells, formed yellow-pigmented colonies on ISP2 medium and its optimum growth pH was 7·0–7·5. The major respiratory lipoquinone was ubiquinone Q-10. The phosphate-containing lipids detected in strain YIM 003T were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, sphingoglycolipid and one unidentified phospholipid. The major fatty acids were C18 : 1 ω7c (59·8 %), C16 : 0 (9·9 %), ai-C17 : 0 (5·3 %), i-C17 : 0 (4·4 %) and C14 : 0 2-OH (15·8 %). The G+C content of the genomic DNA was 67·5 mol%. Strain YIM 003T exhibited levels of 16S rRNA gene sequence similarity of 98·2 % to Sphingomonas phyllosphaerae FA2T and 98·0 % to Sphingomonas adhaesiva DSM 7418T but showed less than 97·0 % similarity with respect to other species with validly published names. The DNA–DNA relatedness values of the isolate with S. phyllosphaerae FA2T and S. adhaesiva DSM 7418T were 59 and 26 %, respectively. The phenotypic characteristics and genotypic data indicate that strain YIM 003T should be distinguished from S. phyllosphaerae FA2T and S. adhaesiva DSM 7418T. Therefore, on the basis of the polyphasic taxonomic data presented, a novel species of the genus Sphingomonas, Sphingomonas yunnanensis sp. nov., is proposed, with the type strain YIM 003T (=CCTCC AB 204064T=KCTC 12346T).
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Sphingomonas yunnanensis sp. nov., a novel Gram-Negative Bacterium from a contaminated plate.
International journal of systematic and evolutionary microbiology, 2005Co-Authors: Yu-qin Zhang, Yi-guang Chen, Xin-peng Tian, Cheng-lin JiangAbstract:A Gram-Negative Bacterium, YIM 003T, which was isolated from a contaminated plate in the laboratory, was subjected to a polyphasic taxonomic study. The organism had short-rod-shaped, motile cells, formed yellow-pigmented colonies on ISP2 medium and its optimum growth pH was 7.0-7.5. The major respiratory lipoquinone was ubiquinone Q-10. The phosphate-containing lipids detected in strain YIM 003T were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, sphingoglycolipid and one unidentified phospholipid. The major fatty acids were C(18 : 1)omega7c (59.8 %), C(16 : 0) (9.9 %), ai-C(17 : 0) (5.3 %), i-C(17 : 0) (4.4 %) and C(14 : 0) 2-OH (15.8 %). The G+C content of the genomic DNA was 67.5 mol%. Strain YIM 003T exhibited levels of 16S rRNA gene sequence similarity of 98.2 % to Sphingomonas phyllosphaerae FA2T and 98.0 % to Sphingomonas adhaesiva DSM 7418T but showed less than 97.0 % similarity with respect to other species with validly published names. The DNA-DNA relatedness values of the isolate with S. phyllosphaerae FA2T and S. adhaesiva DSM 7418T were 59 and 26 %, respectively. The phenotypic characteristics and genotypic data indicate that strain YIM 003T should be distinguished from S. phyllosphaerae FA2T and S. adhaesiva DSM 7418T. Therefore, on the basis of the polyphasic taxonomic data presented, a novel species of the genus Sphingomonas, Sphingomonas yunnanensis sp. nov., is proposed, with the type strain YIM 003T (=CCTCC AB 204064T=KCTC 12346T).
Eric W. Triplett - One of the best experts on this subject based on the ideXlab platform.
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Runella zeae sp. nov., a novel Gram-Negative Bacterium from the stems of surface-sterilized
2016Co-Authors: Zea Mays, Marisa K. Chelius, Jennifer A. Henn, Eric W. TriplettAbstract:A Gram-Negative Bacterium, designated NS12T, was previously isolated from duplicate treatments of surface-sterilized Zea mays stems. The plants were grown in synthetic soil under greenhouse conditions and watered with fertilizer containing no nitrogen. Strain NS12T was not isolated from plants watered with the standard level or 20 % (w/v) of the standard level of nitrogen. Cells were bent rods that formed chains of irregular shapes in R2A broth. Unlike its closest described relative Runella slithyformis, strain NS12T fermented glucose and sucrose. The GMC content was 49 mol%. Phylogenetic analysis of the 16S rRNA gene showed that the strain was a member of the domain Bacteria and is most closely related to R. slithyformis, a member of the Flexibacter group within the Cytophaga–Flexibacter–Bacteroides phylum. Phenotypic and genotypic analyses indicated that strain NS12T could not be assigned to any recognized species; therefore a new species designated Runella zeae sp. nov. is proposed, for which NS12T (flATCC BAA-293T flLMG 21438T) is the type strain
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Runella zeae sp. nov., a novel Gram-Negative Bacterium from the stems of surface-sterilized Zea mays.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2002Co-Authors: Marisa K. Chelius, Jennifer A. Henn, Eric W. TriplettAbstract:A Gram-Negative Bacterium, designated NS12(T), was previously isolated from duplicate treatments of surface-sterilized Zea mays stems. The plants were grown in synthetic soil under greenhouse conditions and watered with fertilizer containing no nitrogen. Strain NS12(T) was not isolated from plants watered with the standard level or 20% (w/v) of the standard level of nitrogen. Cells were bent rods that formed chains of irregular shapes in R2A broth. Unlike its closest described relative Runella slithyformis, strain NS12(T) fermented glucose and sucrose. The G+C content was 49 mol%. Phylogenetic analysis of the 16S rRNA gene showed that the strain was a member of the domain Bacteria and is most closely related to R. slithyformis, a member of the Flexibacter group within the Cytophaga-Flexibacter-Bacteroides phylum. Phenotypic and genotypic analyses indicated that strain NS12(T) could not be assigned to any recognized species; therefore a new species designated Runella zeae sp. nov. is proposed, for which NS12(T) ( = ATCC BAA-293(T) = LMG 21438(T)) is the type strain.
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Dyadobacter fermentans gen. nov., sp. nov., a novel Gram-Negative Bacterium isolated from surface-sterilized Zea mays stems.
International Journal of Systematic and Evolutionary Microbiology, 2000Co-Authors: Marisa K. Chelius, Eric W. TriplettAbstract:A Gram-Negative Bacterium, designated NS114T, was isolated from duplicate treatments of surface-sterilized Zea mays stems. The plants were grown in synthetic soil under greenhouse conditions and watered with fertilizer containing no nitrogen. Strain NS114T could not be isolated from plants watered with the standard level or 20% (w/v) of the standard level of nitrogen. Cells occurred as pairs in young cultures that attached to form angled arrangements in R2A broth and occasionally formed rounded, horseshoe arrangements in YM broth. Cell variation resulted in flocculent chains of coccoid cells in old cultures. Strain NS114T fermented glucose and sucrose. The G + C content was 48 mol%. Phylogenetic analysis of the 16S rRNA gene showed that the strain was a member of the domain Bacteria and branched from a point equidistant from an aquatic organism, Runella slithyformis and a marine isolate, 'Microscilla furvescens'. Phenotypic and genotypic analyses indicated that strain NS114T could not be assigned to any recognized genus; therefore a new genus and species, Dyadobacter fermentans gen. nov., sp. nov., is proposed, for which NS114T is the type strain.
Yu-qin Zhang - One of the best experts on this subject based on the ideXlab platform.
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Sphingomonas yunnanensis sp. nov., a novel Gram-Negative Bacterium from a contaminated plate
International Journal of Systematic and Evolutionary Microbiology, 2005Co-Authors: Yu-qin Zhang, Yi-guang Chen, Xin-peng Tian, Cheng-lin JiangAbstract:A Gram-Negative Bacterium, YIM 003T, which was isolated from a contaminated plate in the laboratory, was subjected to a polyphasic taxonomic study. The organism had short-rod-shaped, motile cells, formed yellow-pigmented colonies on ISP2 medium and its optimum growth pH was 7·0–7·5. The major respiratory lipoquinone was ubiquinone Q-10. The phosphate-containing lipids detected in strain YIM 003T were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, sphingoglycolipid and one unidentified phospholipid. The major fatty acids were C18 : 1 ω7c (59·8 %), C16 : 0 (9·9 %), ai-C17 : 0 (5·3 %), i-C17 : 0 (4·4 %) and C14 : 0 2-OH (15·8 %). The G+C content of the genomic DNA was 67·5 mol%. Strain YIM 003T exhibited levels of 16S rRNA gene sequence similarity of 98·2 % to Sphingomonas phyllosphaerae FA2T and 98·0 % to Sphingomonas adhaesiva DSM 7418T but showed less than 97·0 % similarity with respect to other species with validly published names. The DNA–DNA relatedness values of the isolate with S. phyllosphaerae FA2T and S. adhaesiva DSM 7418T were 59 and 26 %, respectively. The phenotypic characteristics and genotypic data indicate that strain YIM 003T should be distinguished from S. phyllosphaerae FA2T and S. adhaesiva DSM 7418T. Therefore, on the basis of the polyphasic taxonomic data presented, a novel species of the genus Sphingomonas, Sphingomonas yunnanensis sp. nov., is proposed, with the type strain YIM 003T (=CCTCC AB 204064T=KCTC 12346T).
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Sphingomonas yunnanensis sp. nov., a novel Gram-Negative Bacterium from a contaminated plate.
International journal of systematic and evolutionary microbiology, 2005Co-Authors: Yu-qin Zhang, Yi-guang Chen, Xin-peng Tian, Cheng-lin JiangAbstract:A Gram-Negative Bacterium, YIM 003T, which was isolated from a contaminated plate in the laboratory, was subjected to a polyphasic taxonomic study. The organism had short-rod-shaped, motile cells, formed yellow-pigmented colonies on ISP2 medium and its optimum growth pH was 7.0-7.5. The major respiratory lipoquinone was ubiquinone Q-10. The phosphate-containing lipids detected in strain YIM 003T were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, sphingoglycolipid and one unidentified phospholipid. The major fatty acids were C(18 : 1)omega7c (59.8 %), C(16 : 0) (9.9 %), ai-C(17 : 0) (5.3 %), i-C(17 : 0) (4.4 %) and C(14 : 0) 2-OH (15.8 %). The G+C content of the genomic DNA was 67.5 mol%. Strain YIM 003T exhibited levels of 16S rRNA gene sequence similarity of 98.2 % to Sphingomonas phyllosphaerae FA2T and 98.0 % to Sphingomonas adhaesiva DSM 7418T but showed less than 97.0 % similarity with respect to other species with validly published names. The DNA-DNA relatedness values of the isolate with S. phyllosphaerae FA2T and S. adhaesiva DSM 7418T were 59 and 26 %, respectively. The phenotypic characteristics and genotypic data indicate that strain YIM 003T should be distinguished from S. phyllosphaerae FA2T and S. adhaesiva DSM 7418T. Therefore, on the basis of the polyphasic taxonomic data presented, a novel species of the genus Sphingomonas, Sphingomonas yunnanensis sp. nov., is proposed, with the type strain YIM 003T (=CCTCC AB 204064T=KCTC 12346T).
Ofir Bahar - One of the best experts on this subject based on the ideXlab platform.
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tyrosine sulfation in a Gram Negative Bacterium
Nature Communications, 2012Co-Authors: Ofir Bahar, Benjamin Schwessinger, Michelle R Robinson, Jared B Shaw, James A Madsen, Jennifer S Brodbelt, Pamela C RonaldAbstract:The sulfation of protein tyrosine residues is a common post-translational modification in eukaryotes. Here, Han et al. show that the protein RaxST, produced by a plant Bacterium, has tyrosine sulfotransferase activity, demonstrating for the first time tyrosine sulfation in prokaryotes.
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Tyrosine sulfation in a Gram-Negative Bacterium
Nature communications, 2012Co-Authors: Sang-wook Han, Ofir Bahar, Benjamin Schwessinger, Michelle R Robinson, Jared B Shaw, James A Madsen, Jennifer S Brodbelt, Sang Won Lee, Pamela C RonaldAbstract:Tyrosine sulfation, a well-characterized post-translation modification in eukaryotes, has not previously been reported in prokaryotes. Here, we demonstrate that the RaxST protein from the Gram-Negative Bacterium, Xanthomonas oryzae pv. oryzae, is a tyrosine sulfotransferase. We used a newly developed sulfotransferase assay and ultraviolet photodissociation mass spectrometry to demonstrate that RaxST catalyses sulfation of tyrosine 22 of the Xoo Ax21 (activator of XA21-mediated immunity) protein. These results demonstrate a previously undescribed post-translational modification in a prokaryotic species with implications for studies of host immune responses and bacterial cell-cell communication systems.
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Small Protein-Mediated Quorum Sensing in a Gram-Negative Bacterium
PloS one, 2011Co-Authors: Sang-wook Han, Ofir Bahar, Sang Won Lee, Malinee Sriariyanun, Manoj K. Sharma, Zachary Bower, Pamela C RonaldAbstract:The rice XA21 pattern recognition receptor binds a type I secreted sulfated peptide, called axYS22, derived from the Ax21 (activator of XA21-mediated immunity) protein. The conservation of Ax21 in all sequenced Xanthomonas spp. and closely related genera suggests that Ax21 serves a key biological function. Here we show that the predicted N-terminal sequence of Ax21 is cleaved prior to secretion outside the cell and that mature Ax21 serves as a quorum sensing (QS) factor in Xanthomonas oryzae pv. oryzae. Ax21-mediated QS controls motility, biofilm formation and virulence. We provide genetic evidence that the Xoo RaxH histidine kinase serves as the bacterial receptor for Ax21. This work establishes a critical role for small protein-mediated QS in a Gram-Negative Bacterium.
