GRAS Substance

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Xue Li - One of the best experts on this subject based on the ideXlab platform.

  • antifungal activity of e poly l lysine on trichothecium roseum in vitro and its mechanisms
    Physiological and Molecular Plant Pathology, 2018
    Co-Authors: Yonghong Ge, Canying Li, Yanru Chen, Wenhui Wang, Bin Duan, Xue Li
    Abstract:

    Abstract Trichothecium roseum is the main pathogen that causes postharvest decay in several fruits and vegetables. e-poly-L-lysine (PL), generally regarded as a safe (GRAS) Substance, is a cationic homopolymer that consists of e-amino and α-hydroxyl. The effect of PL at different concentrations (0, 12.5, 25. 50, 100, and 200 μL L−1) on the mycelia growth and spore germination of T. roseum was investigated in this study. The mechanisms of PL inhibition on T. roseum growth were also studied. The results indicated that the mycelia growth and spore germination of T. roseum were significantly inhibited at 100 and 200 μL L−1 PL. Electrical conductivity and extracellular protein showed that PL treatment destroyed the plasma membrane integrity of the mycelia and spores, respectively, and decreased extracellular malondialdehyde (MDA) and sugar content after treatment. Scanning electron microscopy indicated that PL treatment damaged spore morphology, and the surface was rough and shrinking. Transmission electron microscopy indicated that PL severely destroyed the cell wall and the plasma membrane of the T. roseum spore, leading to the loss of cytoplasm and cracking of vacuole. These results suggest that PL effectively inhibits the growth of T. roseum, and the mechanism is related to the damage of cell walls and cell membranes.

  • Antifungal activity of ε-poly-L-lysine on Trichothecium roseum in vitro and its mechanisms
    Physiological and Molecular Plant Pathology, 2018
    Co-Authors: Yonghong Ge, Canying Li, Yanru Chen, Wenhui Wang, Bin Duan, Xue Li
    Abstract:

    Abstract Trichothecium roseum is the main pathogen that causes postharvest decay in several fruits and vegetables. e-poly-L-lysine (PL), generally regarded as a safe (GRAS) Substance, is a cationic homopolymer that consists of e-amino and α-hydroxyl. The effect of PL at different concentrations (0, 12.5, 25. 50, 100, and 200 μL L−1) on the mycelia growth and spore germination of T. roseum was investigated in this study. The mechanisms of PL inhibition on T. roseum growth were also studied. The results indicated that the mycelia growth and spore germination of T. roseum were significantly inhibited at 100 and 200 μL L−1 PL. Electrical conductivity and extracellular protein showed that PL treatment destroyed the plasma membrane integrity of the mycelia and spores, respectively, and decreased extracellular malondialdehyde (MDA) and sugar content after treatment. Scanning electron microscopy indicated that PL treatment damaged spore morphology, and the surface was rough and shrinking. Transmission electron microscopy indicated that PL severely destroyed the cell wall and the plasma membrane of the T. roseum spore, leading to the loss of cytoplasm and cracking of vacuole. These results suggest that PL effectively inhibits the growth of T. roseum, and the mechanism is related to the damage of cell walls and cell membranes.

Yonghong Ge - One of the best experts on this subject based on the ideXlab platform.

  • antifungal activity of e poly l lysine on trichothecium roseum in vitro and its mechanisms
    Physiological and Molecular Plant Pathology, 2018
    Co-Authors: Yonghong Ge, Canying Li, Yanru Chen, Wenhui Wang, Bin Duan, Xue Li
    Abstract:

    Abstract Trichothecium roseum is the main pathogen that causes postharvest decay in several fruits and vegetables. e-poly-L-lysine (PL), generally regarded as a safe (GRAS) Substance, is a cationic homopolymer that consists of e-amino and α-hydroxyl. The effect of PL at different concentrations (0, 12.5, 25. 50, 100, and 200 μL L−1) on the mycelia growth and spore germination of T. roseum was investigated in this study. The mechanisms of PL inhibition on T. roseum growth were also studied. The results indicated that the mycelia growth and spore germination of T. roseum were significantly inhibited at 100 and 200 μL L−1 PL. Electrical conductivity and extracellular protein showed that PL treatment destroyed the plasma membrane integrity of the mycelia and spores, respectively, and decreased extracellular malondialdehyde (MDA) and sugar content after treatment. Scanning electron microscopy indicated that PL treatment damaged spore morphology, and the surface was rough and shrinking. Transmission electron microscopy indicated that PL severely destroyed the cell wall and the plasma membrane of the T. roseum spore, leading to the loss of cytoplasm and cracking of vacuole. These results suggest that PL effectively inhibits the growth of T. roseum, and the mechanism is related to the damage of cell walls and cell membranes.

  • Antifungal activity of ε-poly-L-lysine on Trichothecium roseum in vitro and its mechanisms
    Physiological and Molecular Plant Pathology, 2018
    Co-Authors: Yonghong Ge, Canying Li, Yanru Chen, Wenhui Wang, Bin Duan, Xue Li
    Abstract:

    Abstract Trichothecium roseum is the main pathogen that causes postharvest decay in several fruits and vegetables. e-poly-L-lysine (PL), generally regarded as a safe (GRAS) Substance, is a cationic homopolymer that consists of e-amino and α-hydroxyl. The effect of PL at different concentrations (0, 12.5, 25. 50, 100, and 200 μL L−1) on the mycelia growth and spore germination of T. roseum was investigated in this study. The mechanisms of PL inhibition on T. roseum growth were also studied. The results indicated that the mycelia growth and spore germination of T. roseum were significantly inhibited at 100 and 200 μL L−1 PL. Electrical conductivity and extracellular protein showed that PL treatment destroyed the plasma membrane integrity of the mycelia and spores, respectively, and decreased extracellular malondialdehyde (MDA) and sugar content after treatment. Scanning electron microscopy indicated that PL treatment damaged spore morphology, and the surface was rough and shrinking. Transmission electron microscopy indicated that PL severely destroyed the cell wall and the plasma membrane of the T. roseum spore, leading to the loss of cytoplasm and cracking of vacuole. These results suggest that PL effectively inhibits the growth of T. roseum, and the mechanism is related to the damage of cell walls and cell membranes.

Novalia Rachmawati - One of the best experts on this subject based on the ideXlab platform.

  • PENGGUNAAN CETYLPERIDINIUM CHLORIDE SEBAGAI ANTI BAKTERI PADA UDANG Application of Cetylperidinium Chloride (CPC) as Anti Bacterial Agent on Shrimp
    2020
    Co-Authors: Radestya Triwibowo, Novalia Rachmawati
    Abstract:

    Application of Cetylperidinium Chloride (CPC) as anti bacterial agent has been done on artificially contaminated shrimp. Cetylperidinium Chloride application in food products has been permitted by The Food and Drug Administration (FDA) and European Food Safety Authority (EFSA) since it has been categorized as a Generally Recognized As Safe (GRAS) Substance. Pathogenic bacteria used were E. coli, Salmonella sp. and Vibrio parahaemolyticus with cell density of 0.5 McFarland. Concentrations of CPC solution were based on a preliminary experiment, i.e. 0.4; 0.8; 1.2% (w/v) and aquadest was used as control. Shrimp was dipped into the solution for approximately 1 minute, dried for 10 minutes and packed. Shrimp was then stored at ±4 oC and ±25 oC. The growth of pathogenic bacteria was analyzed periodically. The result showed that CPC was able to prevent the growth of E. coli and Vibrio parahaemolyticus on shrimp stored at ±4 oC and ±25 oC. However, the growth of Salmonella sp. was prevented on shrimp stored at ±4 oC only. Cetylperidinium Chloride at the concentration of 0.8 and 1.2% had the same effectivity, therefore the recommended concentration of CPC is 0.8%.

Bin Duan - One of the best experts on this subject based on the ideXlab platform.

  • antifungal activity of e poly l lysine on trichothecium roseum in vitro and its mechanisms
    Physiological and Molecular Plant Pathology, 2018
    Co-Authors: Yonghong Ge, Canying Li, Yanru Chen, Wenhui Wang, Bin Duan, Xue Li
    Abstract:

    Abstract Trichothecium roseum is the main pathogen that causes postharvest decay in several fruits and vegetables. e-poly-L-lysine (PL), generally regarded as a safe (GRAS) Substance, is a cationic homopolymer that consists of e-amino and α-hydroxyl. The effect of PL at different concentrations (0, 12.5, 25. 50, 100, and 200 μL L−1) on the mycelia growth and spore germination of T. roseum was investigated in this study. The mechanisms of PL inhibition on T. roseum growth were also studied. The results indicated that the mycelia growth and spore germination of T. roseum were significantly inhibited at 100 and 200 μL L−1 PL. Electrical conductivity and extracellular protein showed that PL treatment destroyed the plasma membrane integrity of the mycelia and spores, respectively, and decreased extracellular malondialdehyde (MDA) and sugar content after treatment. Scanning electron microscopy indicated that PL treatment damaged spore morphology, and the surface was rough and shrinking. Transmission electron microscopy indicated that PL severely destroyed the cell wall and the plasma membrane of the T. roseum spore, leading to the loss of cytoplasm and cracking of vacuole. These results suggest that PL effectively inhibits the growth of T. roseum, and the mechanism is related to the damage of cell walls and cell membranes.

  • Antifungal activity of ε-poly-L-lysine on Trichothecium roseum in vitro and its mechanisms
    Physiological and Molecular Plant Pathology, 2018
    Co-Authors: Yonghong Ge, Canying Li, Yanru Chen, Wenhui Wang, Bin Duan, Xue Li
    Abstract:

    Abstract Trichothecium roseum is the main pathogen that causes postharvest decay in several fruits and vegetables. e-poly-L-lysine (PL), generally regarded as a safe (GRAS) Substance, is a cationic homopolymer that consists of e-amino and α-hydroxyl. The effect of PL at different concentrations (0, 12.5, 25. 50, 100, and 200 μL L−1) on the mycelia growth and spore germination of T. roseum was investigated in this study. The mechanisms of PL inhibition on T. roseum growth were also studied. The results indicated that the mycelia growth and spore germination of T. roseum were significantly inhibited at 100 and 200 μL L−1 PL. Electrical conductivity and extracellular protein showed that PL treatment destroyed the plasma membrane integrity of the mycelia and spores, respectively, and decreased extracellular malondialdehyde (MDA) and sugar content after treatment. Scanning electron microscopy indicated that PL treatment damaged spore morphology, and the surface was rough and shrinking. Transmission electron microscopy indicated that PL severely destroyed the cell wall and the plasma membrane of the T. roseum spore, leading to the loss of cytoplasm and cracking of vacuole. These results suggest that PL effectively inhibits the growth of T. roseum, and the mechanism is related to the damage of cell walls and cell membranes.

Wenhui Wang - One of the best experts on this subject based on the ideXlab platform.

  • antifungal activity of e poly l lysine on trichothecium roseum in vitro and its mechanisms
    Physiological and Molecular Plant Pathology, 2018
    Co-Authors: Yonghong Ge, Canying Li, Yanru Chen, Wenhui Wang, Bin Duan, Xue Li
    Abstract:

    Abstract Trichothecium roseum is the main pathogen that causes postharvest decay in several fruits and vegetables. e-poly-L-lysine (PL), generally regarded as a safe (GRAS) Substance, is a cationic homopolymer that consists of e-amino and α-hydroxyl. The effect of PL at different concentrations (0, 12.5, 25. 50, 100, and 200 μL L−1) on the mycelia growth and spore germination of T. roseum was investigated in this study. The mechanisms of PL inhibition on T. roseum growth were also studied. The results indicated that the mycelia growth and spore germination of T. roseum were significantly inhibited at 100 and 200 μL L−1 PL. Electrical conductivity and extracellular protein showed that PL treatment destroyed the plasma membrane integrity of the mycelia and spores, respectively, and decreased extracellular malondialdehyde (MDA) and sugar content after treatment. Scanning electron microscopy indicated that PL treatment damaged spore morphology, and the surface was rough and shrinking. Transmission electron microscopy indicated that PL severely destroyed the cell wall and the plasma membrane of the T. roseum spore, leading to the loss of cytoplasm and cracking of vacuole. These results suggest that PL effectively inhibits the growth of T. roseum, and the mechanism is related to the damage of cell walls and cell membranes.

  • Antifungal activity of ε-poly-L-lysine on Trichothecium roseum in vitro and its mechanisms
    Physiological and Molecular Plant Pathology, 2018
    Co-Authors: Yonghong Ge, Canying Li, Yanru Chen, Wenhui Wang, Bin Duan, Xue Li
    Abstract:

    Abstract Trichothecium roseum is the main pathogen that causes postharvest decay in several fruits and vegetables. e-poly-L-lysine (PL), generally regarded as a safe (GRAS) Substance, is a cationic homopolymer that consists of e-amino and α-hydroxyl. The effect of PL at different concentrations (0, 12.5, 25. 50, 100, and 200 μL L−1) on the mycelia growth and spore germination of T. roseum was investigated in this study. The mechanisms of PL inhibition on T. roseum growth were also studied. The results indicated that the mycelia growth and spore germination of T. roseum were significantly inhibited at 100 and 200 μL L−1 PL. Electrical conductivity and extracellular protein showed that PL treatment destroyed the plasma membrane integrity of the mycelia and spores, respectively, and decreased extracellular malondialdehyde (MDA) and sugar content after treatment. Scanning electron microscopy indicated that PL treatment damaged spore morphology, and the surface was rough and shrinking. Transmission electron microscopy indicated that PL severely destroyed the cell wall and the plasma membrane of the T. roseum spore, leading to the loss of cytoplasm and cracking of vacuole. These results suggest that PL effectively inhibits the growth of T. roseum, and the mechanism is related to the damage of cell walls and cell membranes.