The Experts below are selected from a list of 246 Experts worldwide ranked by ideXlab platform
Yang Bi - One of the best experts on this subject based on the ideXlab platform.
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early defense responses involved in mitochondrial energy metabolism and reactive oxygen species accumulation in harvested muskmelons infected by Trichothecium roseum
Journal of Agricultural and Food Chemistry, 2019Co-Authors: Yang Bi, Shenge Li, Zhong Zhang, Dov PruskyAbstract:Mitochondria play an essential part in fighting against pathogen infection in the defense responses of fruits. In this study, we investigated the reactive oxygen species (ROS) production, energy metabolism, and changes of mitochondrial proteins in harvested muskmelon fruits (Cucumis melo cv. Yujinxiang) inoculated with Trichothecium roseum. The results indicated that the fungal infection obviously induced the H2O2 accumulation in mitochondria. Enzyme activities were inhibited in the first 6 h postinoculation (hpi), including succinic dehydrogenase, cytochrome c oxidase, H+-ATPase, and Ca2+-ATPase. However, the activities of Ca2+-ATPase and H+-ATPase and the contents of intracellular adenosine triphosphate (ATP) were improved to a higher level at 12 hpi. A total of 42 differentially expressed proteins were identified through tandem mass tags-based proteomic analyses, which are mainly involved in energy metabolism, stress responses and redox homeostasis, glycolysis and tricarboxylic acid cycle, and transpor...
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influence of storage temperature and cultivars on t 2 toxin and neosolaniol accumulation in apples inoculated with Trichothecium roseum
Journal of Food Processing and Preservation, 2018Co-Authors: Yang Bi, Y Tang, S Hukkeri, X Li, L Pu, G Fu, Yi Wang, Y LiAbstract:Trichothecium roseum is one of the most dreadful postharvest pathogens responsible for core rot of apples. Besides causing economic loss, the disease is also associated with mycotoxins contamination. The effects of storage temperature and lesion diameter on T-2 toxin and neosolaniol (NEO) accumulation were evaluated in genetically diverse apple cultivars. The results showed T-2 concentration was much higher in Fuji than that in Ralls, while NEO was only detected in Red Delicious. The room temperature is favorable for T-2 and NEO accumulation compared with low temperature. We also noticed the positive relationship between the size of lesion diameter and the concentrations of NEO and T-2. NEO and T-2 were found not only in the rotten part, but in the adjacent asymptomatic tissue, indicating that the spread of the trichothecenes to non-infected of the fruits, and trichothecenes concentration showed a trend of decline with increase in distance from the rotten parts. Practical application Trichothecium roseum is one of the most important postharvest pathogens, and can cause core rot of apples. The disease not only causes economic loss, but also results in trichothecene contamination. Storage temperature and the size of lesion diameter significantly affected T-2 and neosolaniol accumulation in genetically diverse apple. The results showed T-2 toxin concentration was much higher in Fuji than that in Ralls, while neosolaniol was only detected in Red Delicious. The room temperature was favorable for T-2 toxin and neosolaniol accumulation compared with low temperature. In addition, the positive relationship between the size of lesion diameter and the concentrations of neosolaniol and T-2 toxin was observed. T-2 toxin and neosolaniol were found not only in the rotten part, but also in the adjacent asymptomatic tissue, and the concentration showed a trend of decline with increase in distance from the rotten parts. The study will be useful not only for apple post-harvest storage and reduce the health risk of exposure to the trichothecenes of T-2 toxin and neosolaniol, but also provide baseline information for the introduction of EU-limits for T-2 toxin and neosolaniol.
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damage to Trichothecium roseum caused by sodium silicate is independent from ph
Canadian Journal of Microbiology, 2016Co-Authors: Yang Bi, Yi Wang, Shenggui Zhang, Yongcai Li, Alejandro CalderonurreaAbstract:Trichothecium roseum is one of the most important postharvest pathogens in arid and semiarid regions. Sodium silicate (NaSi) and environmental pH have significant inhibitory effects on fungal growth. However, no study has addressed the relationship of NaSi and pH in combination and the effects on T. roseum. In this work, we showed that spore germination, germ tube elongation, and mycelial growth of T. roseum were significantly inhibited by various NaSi concentrations, which had corresponding increasing pHs. Furthermore, these NaSi solutions showed a much greater impact than did pH treatments alone. The pathogenicity of NaSi-treated conidia on a model assay (conidia-inoculated apple fruit) was dramatically reduced, whereas no changes of pathogenicity were evident for the corresponding pH (various sodium hydroxide (NaOH) solutions) treatments. Fluorescent microscopy, using propidium iodide staining, showed damage of the plasma membranes of T. roseum conidia treated with both NaSi and NaOH, although the dama...
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Use of silicon oxide and sodium silicate for controlling Trichothecium roseum postharvest rot in Chinese cantaloupe (Cucumis melo L.)
International Journal of Food Science and Technology, 2007Co-Authors: Jian Zhao, Yang BiAbstract:Summary Silicon oxide and sodium silicate were investigated as potential agents for the control of postharvest pink rot in Chinese cantaloupe (cultivar Yujingxing) caused by Trichothecium roseum. In vitro tests showed that sodium silicate, when added to potato dextrose agar, was effective in suppressing the radial growth of the pathogen on the medium, whereas silicon oxide was ineffective. The effectiveness of sodium silicate increased with concentration, and the growth of the fungus was completely inhibited at 100 mm. When melons were dipped in the solutions, both silicon oxide and sodium silicate significantly (P
Xiaomei Zhang - One of the best experts on this subject based on the ideXlab platform.
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endophytic fungus Trichothecium roseum lz93 antagonizing pathogenic fungi in vitro and its secondary metabolites
Journal of Microbiology, 2010Co-Authors: Xiaomei Zhang, Guohong Li, Ying Zeng, Peiji ZhaoAbstract:The endophytic fungus Trichothecium roseum LZ93 from Maytenus hookeri was found to antagonize other pathogenic fungi in vitro. To identify which compound contributed substantially to the antagonism, we fermented the strain and purified its fermentation products. Eleven compounds were obtained, including two trichothecenes, five rosenonolactones, two cardiotonic cyclodepsipeptides, and two sterols. Compound 11β-hydroxyrosenonolactone (1) was assigned according to 1D and 2D-NMR data for the first time. At the same time, the 1H and 13C-NMR assignments for 6β-hydroxyrosenonolactone (2) were revised. Of all of them, only trichothecin (6) showed strong antifungal activity. Based on our observations of the antagonistic activity and the other experimental results, we suggest that the antifungal compound trichothecin was the main contributor to the antagonistic action of T. roseum LZ93.
Genuino Negri - One of the best experts on this subject based on the ideXlab platform.
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effect of Trichothecium roseum lime sulphur and phosphites to control blossom blight and brown rot on peach
Canadian Journal of Plant Pathology-revue Canadienne De Phytopathologie, 2014Co-Authors: Genuino Negri, Themis J MichailidesAbstract:AbstractThis study assessed the efficacy of phosphites, Trichothecium roseum (TR) and a combination of phosphites and TR compared with lime sulphur (LS) to control brown rot, caused by Monilinia fructicola, in an organic orchard with high inoculum pressure under subtropical conditions in Brazil. The treatments were applied from blossom to harvest on two peach cultivars (‘Granada’ and ‘Chimarrita’) during 2005 and 2006. Disease incidence was assessed from full bloom to post-harvest. Two other experiments were performed under post-harvest conditions using mature fruit harvested from organic and conventional orchards and inoculated with 106 conidia mL−1 of TR followed by M. fructicola. The incidence of blossom blight ranged from 25% to 64% on flowers collected from untreated controls, whereas all treatments reduced blight over both cultivars and years. For ‘Chimarrita’, disease incidence reached 8% in mature fruit on the tree and 43% on fruit post-harvest. In both years, TR treatment reduced fruit infection ...
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producao e armazenamento de Trichothecium roseum para uso como biopesticida
Random Structures and Algorithms, 2010Co-Authors: Genuino Negri, Joao Americo Wordell FilhoAbstract:Trichothecium roseum is a potential antagonistic fungus to control peach Brown rot reducing the pathogen infection and disease damage. The current experiment aimed at developing a methodology for production of the antagonist Trichothecium roseum in different substrates, and to test its viability when stored in several temperatures. The substrates used were brown rice, rice hulls, sorghum grain and wheat grain boiled to tap water for 5, 10 and 20 min and not boiled, with and without added of nutrients with four repetitions. The evaluation of the stored inoculum viability was tested in temperatures: -4, 4, 15, 25, 35 o C and room temperature, for 90 days. The analysis of variance indicated significant interactions among the substrates, the time of boiling and the types of treatment with or without the use of nutrients. The rice and wheat substrates without PD (potato + dextrose) nutrients addition were the more efficients in the production of the antagonist, demonstrating that these do not require the addition of nutrients for multiplication of the fungus. To maintain the viability of the inoculum The best storage condition was at temperature -4 o C, where of rates in viability of the conidia were 74% at 30 days, 44% at 60 days and 32% at 90 days; this temperature can easily be obtained in freezers; therefore, future users do not need to make high investments in equipment for the conservation of the antagonist.
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producao e armazenamento de Trichothecium roseum para uso como biopesticida production and storage of Trichothecium roseum to use as biopesticide
2010Co-Authors: Genuino Negri, Louise LarissaAbstract:Trichothecium roseum is a potential antagonistic fungus to control peach Brown rot reducing the pathogen infection and disease damage. The current experiment aimed at developing a methodology for production of the antagonist Trichothecium roseum in different substrates, and to test its viability when stored in several temperatures. The substrates used were brown rice, rice hulls, sorghum grain and wheat grain boiled to tap water for 5, 10 and 20 min and not boiled, with and without added of nutrients with four repetitions. The evaluation of the stored inoculum viability was tested in temperatures: -4, 4, 15, 25, 35 o C and room temperature, for 90 days. The analysis of variance indicated significant interactions among the substrates, the time of boiling and the types of treatment with or without the use of nutrients. The rice and wheat substrates without PD (potato + dextrose) nutrients addition were the more efficients in the production of the antagonist, demonstrating that these do not require the addition of nutrients for multiplication of the fungus. To maintain the viability of the inoculum The best storage condition was at temperature -4 o C, where of rates in viability of the conidia were 74% at 30 days, 44% at 60 days and 32% at 90 days; this temperature can easily be obtained in freezers; therefore, future users do not need to make high investments in equipment for the conservation of the antagonist.
Peiji Zhao - One of the best experts on this subject based on the ideXlab platform.
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endophytic fungus Trichothecium roseum lz93 antagonizing pathogenic fungi in vitro and its secondary metabolites
Journal of Microbiology, 2010Co-Authors: Xiaomei Zhang, Guohong Li, Ying Zeng, Peiji ZhaoAbstract:The endophytic fungus Trichothecium roseum LZ93 from Maytenus hookeri was found to antagonize other pathogenic fungi in vitro. To identify which compound contributed substantially to the antagonism, we fermented the strain and purified its fermentation products. Eleven compounds were obtained, including two trichothecenes, five rosenonolactones, two cardiotonic cyclodepsipeptides, and two sterols. Compound 11β-hydroxyrosenonolactone (1) was assigned according to 1D and 2D-NMR data for the first time. At the same time, the 1H and 13C-NMR assignments for 6β-hydroxyrosenonolactone (2) were revised. Of all of them, only trichothecin (6) showed strong antifungal activity. Based on our observations of the antagonistic activity and the other experimental results, we suggest that the antifungal compound trichothecin was the main contributor to the antagonistic action of T. roseum LZ93.
Nicholas H Oberlies - One of the best experts on this subject based on the ideXlab platform.
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cyclodepsipeptides sesquiterpenoids and other cytotoxic metabolites from the filamentous fungus Trichothecium sp msx 51320
Journal of Natural Products, 2011Co-Authors: Arlene A Sycordero, Tyler N Graf, Audrey F Adcock, David J Kroll, Qi Shen, Steven M Swanson, Mansukh C Wani, Cedric J Pearce, Nicholas H OberliesAbstract:Two new cyclodepsipeptides (1 and 2), two new sesquiterpenoids (3 and 4), and the known compounds guangomide A (5), roseotoxin S, and three simple trichothecenes were isolated from the cytotoxic organic extract of a terrestrial filamentous fungus, Trichothecium sp. The structures were determined using NMR spectroscopy and mass spectrometry. Absolute configurations of the cyclodepsipeptides were established by employing chiral HPLC, while the relative configurations of 3 and 4 were determined via NOESY data. The isolation of guangomide A was of particular interest, since it was reported previously from a marine-derived fungus.
