The Experts below are selected from a list of 168 Experts worldwide ranked by ideXlab platform
Rekha S. Singhal - One of the best experts on this subject based on the ideXlab platform.
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Solid‐State Fermentation for Production of Griseofulvin on Rice Bran Using Penicillium griseofulvum
Biotechnology Progress, 2008Co-Authors: Sayali S Saykhedkar, Rekha S. SinghalAbstract:: Griseofulvin is a secondary metabolite produced from fungal species that have morphology suitable for solid-state fermentation (SSF). Reports on production of Griseofulvin by SSF are scarce. The present work investigates SSF for Griseofulvin production, optimization of its process parameters vis-a-vis the conventional submerged fermentation and its downstream processing from the same. Rice bran adjusted to an initial moisture content (IMC) of 50% (v/w) inoculated with 1 mL of a suspension of 10(6) spores/mL under agitation at 250 rpm containing the modified Czapek-Dox medium and additional 0.1% choline chloride as a precursor gave a yield of Griseofulvin in 9 days that was comparable to submerged fermentation after 28 days. The yield of Griseofulvin (microg/g dry biomass) was comparable in SSF and submerged fermentation. The biomass was estimated by estimation of chitin. Discussions on the effect of each parameter in SSF have also been included.
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solid state fermentation for production of Griseofulvin on rice bran using penicillium griseofulvum
Biotechnology Progress, 2004Co-Authors: Sayali S Saykhedkar, Rekha S. SinghalAbstract:: Griseofulvin is a secondary metabolite produced from fungal species that have morphology suitable for solid-state fermentation (SSF). Reports on production of Griseofulvin by SSF are scarce. The present work investigates SSF for Griseofulvin production, optimization of its process parameters vis-a-vis the conventional submerged fermentation and its downstream processing from the same. Rice bran adjusted to an initial moisture content (IMC) of 50% (v/w) inoculated with 1 mL of a suspension of 10(6) spores/mL under agitation at 250 rpm containing the modified Czapek-Dox medium and additional 0.1% choline chloride as a precursor gave a yield of Griseofulvin in 9 days that was comparable to submerged fermentation after 28 days. The yield of Griseofulvin (microg/g dry biomass) was comparable in SSF and submerged fermentation. The biomass was estimated by estimation of chitin. Discussions on the effect of each parameter in SSF have also been included.
Sayali S Saykhedkar - One of the best experts on this subject based on the ideXlab platform.
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Solid‐State Fermentation for Production of Griseofulvin on Rice Bran Using Penicillium griseofulvum
Biotechnology Progress, 2008Co-Authors: Sayali S Saykhedkar, Rekha S. SinghalAbstract:: Griseofulvin is a secondary metabolite produced from fungal species that have morphology suitable for solid-state fermentation (SSF). Reports on production of Griseofulvin by SSF are scarce. The present work investigates SSF for Griseofulvin production, optimization of its process parameters vis-a-vis the conventional submerged fermentation and its downstream processing from the same. Rice bran adjusted to an initial moisture content (IMC) of 50% (v/w) inoculated with 1 mL of a suspension of 10(6) spores/mL under agitation at 250 rpm containing the modified Czapek-Dox medium and additional 0.1% choline chloride as a precursor gave a yield of Griseofulvin in 9 days that was comparable to submerged fermentation after 28 days. The yield of Griseofulvin (microg/g dry biomass) was comparable in SSF and submerged fermentation. The biomass was estimated by estimation of chitin. Discussions on the effect of each parameter in SSF have also been included.
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solid state fermentation for production of Griseofulvin on rice bran using penicillium griseofulvum
Biotechnology Progress, 2004Co-Authors: Sayali S Saykhedkar, Rekha S. SinghalAbstract:: Griseofulvin is a secondary metabolite produced from fungal species that have morphology suitable for solid-state fermentation (SSF). Reports on production of Griseofulvin by SSF are scarce. The present work investigates SSF for Griseofulvin production, optimization of its process parameters vis-a-vis the conventional submerged fermentation and its downstream processing from the same. Rice bran adjusted to an initial moisture content (IMC) of 50% (v/w) inoculated with 1 mL of a suspension of 10(6) spores/mL under agitation at 250 rpm containing the modified Czapek-Dox medium and additional 0.1% choline chloride as a precursor gave a yield of Griseofulvin in 9 days that was comparable to submerged fermentation after 28 days. The yield of Griseofulvin (microg/g dry biomass) was comparable in SSF and submerged fermentation. The biomass was estimated by estimation of chitin. Discussions on the effect of each parameter in SSF have also been included.
Mohamed H M Elkomy - One of the best experts on this subject based on the ideXlab platform.
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efficacy of topical Griseofulvin in treatment of tinea corporis
Mycoses, 2006Co-Authors: Mohamed A Kassem, Samia Esmat, Eihab R Bendas, Mohamed H M ElkomyAbstract:Tinea infections are among the most common dermatological conditions throughout the world. Griseofulvin is a classical oral fungistatic antibiotic, active against Epidermophyton floccosum, Trichophyton and Microsporum species, the causative fungi of tinea corporis. To evaluate the efficacy of topical Griseofulvin in the treatment of tinea circinata using three different vehicles for drug delivery. Sixteen patients with tinea circinata were instructed to apply either Griseofulvin gel form in group A or a similar placebo gel for control group; a niosomal gel formulation of Griseofulvin for group B or; a liposomal gel formulation of Griseofulvin for group C. Patients were evaluated both clinically and mycologically after 3 weeks. Marked improvement was seen for groups A, B and C both clinically and mycologically while no improvement was observed in the placebo group. Mild and transient irritation was reported in four patients. Our results show that topical Griseofulvin preparations may be effective and safe in treating tinea circinata and that further large-scale studies may establish the high efficacy of the niosomal gel formulation.
Richard F. Ludueña - One of the best experts on this subject based on the ideXlab platform.
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Griseofulvin: Interaction with normal and subtilisin‐treated tubulin
Drug Development Research, 2001Co-Authors: Asish R. Chaudhuri, Richard F. LudueñaAbstract:Griseofulvin, an antifungal drug, possesses antimitotic activity by inhibiting microtubule assembly. The interaction of Griseofulvin with tubulin is unique in that it increases the sulfhydryl titer of tubulin without affecting the hydrophobic areas. Because the C-terminal regions of both α- and β-tubulin influence the conformational and the drug-binding properties of tubulin, we decided to study the interaction of Griseofulvin with αβ tubulin and tubulin treated with subtilisin to selectively remove the C-terminal regions from both the α- and β-subunits (αsβs). We found that the apparent Kd of Griseofulvin for αsβs tubulin (83 μM) was virtually unaltered compared to its Kd for αβ tubulin (91 μM) as determined by the tryptophan fluorescence quenching assay. The increment of the sulfhydryl titer (0.47 mol/mol) by Griseofulvin was not affected by removing the C-termini from the α- and β-subunits. Moreover, the lack of effect of Griseofulvin on the hydrophobic areas was not changed after the cleavage of the C-termini of the α- and β-subunits. These data therefore strongly suggest that Griseofulvin binds at a certain region of tubulin distant from the C-terminal domains of the α- and β-subunits and that the C-terminal domains of both subunits do not have any conformational influence over the binding site of Griseofulvin. Determining the binding site of Griseofulvin will help in understanding its role in regulating microtubule assembly and dynamics. Drug Dev. Res. 53:44–49, 2001. © 2001 Wiley-Liss, Inc.
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Griseofulvin: A novel interaction with bovine brain tubulin
Biochemical pharmacology, 1996Co-Authors: Asish R. Chaudhuri, Richard F. LudueñaAbstract:Griseofulvin is an anti-fungal drug whose mechanism of action is directed against microtubules. Although it inhibits the assembly of mammalian brain tubulin, its binding to tubulin has not been directly measured successfully. We have examined the interaction of Griseofulvin with tubulin fluorometrically by measuring the quenching of tubulin tryptophan fluorescence by Griseofulvin. From Scatchard analysis, we found that Griseofulvin bound to tubulin at one class of binding site with an affinity constant of 1.2 +/- 0.19 x 10(4) M(-1), and the binding was largely reversible. Griseofulvin caused a major change in the conformation of tubulin in that it increased the sulfhydryl titer of tubulin approximately 2-fold. The drug affected both the alpha and beta subunits of tubulin equally. Interestingly, Griseofulvin did not increase the sulfhydryl titer of the tubulin-colchicine complex although the binding site of Griseofulvin was distinctly different from that of colchicine. The change of conformation of tubulin upon interaction with Griseofulvin did not affect the exposure of hydrophobic areas on tubulin as shown by binding of bis-5,5'-[8(N-phenyl)aminonapthalene-1-sulfonic acid] (BisANS). Even in combination with colchicine, Griseofulvin had very little effect on BisANS binding to tubulin. Thus, Griseofulvin appears to interact with tubulin in a manner that is very different from that of many other tubulin ligands.
Mohamed A Kassem - One of the best experts on this subject based on the ideXlab platform.
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efficacy of topical Griseofulvin in treatment of tinea corporis
Mycoses, 2006Co-Authors: Mohamed A Kassem, Samia Esmat, Eihab R Bendas, Mohamed H M ElkomyAbstract:Tinea infections are among the most common dermatological conditions throughout the world. Griseofulvin is a classical oral fungistatic antibiotic, active against Epidermophyton floccosum, Trichophyton and Microsporum species, the causative fungi of tinea corporis. To evaluate the efficacy of topical Griseofulvin in the treatment of tinea circinata using three different vehicles for drug delivery. Sixteen patients with tinea circinata were instructed to apply either Griseofulvin gel form in group A or a similar placebo gel for control group; a niosomal gel formulation of Griseofulvin for group B or; a liposomal gel formulation of Griseofulvin for group C. Patients were evaluated both clinically and mycologically after 3 weeks. Marked improvement was seen for groups A, B and C both clinically and mycologically while no improvement was observed in the placebo group. Mild and transient irritation was reported in four patients. Our results show that topical Griseofulvin preparations may be effective and safe in treating tinea circinata and that further large-scale studies may establish the high efficacy of the niosomal gel formulation.
