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Hideaki Takahashi - One of the best experts on this subject based on the ideXlab platform.
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the a allele of the cholecystokinin type a receptor gene g 420 c a polymorphism improves Growth Traits in amakusa daioh cross chicken
Journal of Poultry Science, 2019Co-Authors: Hideaki Takahashi, Miya Katayama, Kotoyo Michishita, Hiroaki YamashitaAbstract:Amakusa Daioh cross chickens are F1 hybrids of restored Amakusa Daioh sires and Kyushu Rhode dams. In the present study, the association between a single nucleotide polymorphism (SNP; AB604331, g.420 C>A) in the cholecystokinin type A receptor gene and Growth Traits in Amakusa Daioh cross chicken were investigated. We used 72 male and 72 female birds that had hatched on the same day, were raised in the same chicken house, and were fed the same diet ad libitum from day 0 to 17 weeks (wks) of age. Body weight was recorded at weekly intervals and average daily gain of each week interval was calculated from body weight data. Birds were sacrificed at 17 wks and carcass Traits were recorded. SNP genotyping was carried out using the mismatch amplification mutation assay. Associations between the SNP and Growth Traits were analyzed by a generalized linear model. Body weight from 6 to 17 wks was higher in birds with the A allele than in birds with the C allele, although significant differences in average daily gain Traits between birds with A and C alleles were not detected during most of the duration of the experiment. Carcass data showed that birds with the A allele had heavier wings and a smaller proportion of the gizzard than those with the C allele. The g.420 C>A SNP will be useful as a selection marker for parent stock lines to increase the Growth performance of Amakusa Daioh cross chickens.
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effect of a single nucleotide polymorphism in the cholecystokinin type a receptor gene on Growth Traits of the miyazaki jitokko chicken
Journal of Poultry Science, 2019Co-Authors: Shojiroh Horinouchi, Hiromi Nakayama, Hideaki TakahashiAbstract:The Miyazaki Jitokko chicken, native to the Miyazaki Prefecture in southern Kyushu Island, Japan, is the product of a three-way cross involving the Jitokko, White Plymouth Rock, and Kyushu Rhode breeds. In the present study, associations between a single nucleotide polymorphism (SNP; AB604331, g.420 C>A) of the chicken cholecystokinin type A receptor gene and Growth Traits in Miyazaki Jitokko chickens were investigated. Unrelated male birds (n=120) that had hatched on the same day were raised in the same chicken house and fed the same diet ad libitum from day 0 to 17 weeks of age. Body weight was recorded at 0, 1, 2, 3, 4, 5, 7, 9, 11, 13, 15, and 17 weeks and the average daily gain of each interval was calculated from the body weight data. SNP genotyping of each bird was performed using the mismatch amplification mutation assay. The associations between the SNP and Growth Traits were examined using the Thesias program. The genotype frequencies of AA, AC, and CC were 0.525, 0.383, and 0.092, respectively. AA birds were significantly heavier than CC birds from 4 to 17 weeks of age. In the estimated effect of alleles, body weight from 1 to 17 weeks of age in birds with the A allele was greater than that in birds with the C allele. During the rearing period, the effect of the A allele on average daily gain in the first half was greater than that in the second half. We conclude that the g.420 C>A SNP can be used as a selection marker for the parent stock lines of the Miyazaki Jitokko chickens to increase their Growth performance.
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association between motilin receptor gene haplotypes and Growth Traits in japanese hinai dori crossbred chickens
Asian-australasian Journal of Animal Sciences, 2014Co-Authors: Hideaki Takahashi, Kazuhiro Rikimaru, Megumi Komatsu, Yoshinobu Uemoto, Keiichi SuzukiAbstract:We previously identified quantitative trait loci (QTL) for body weight and average daily gain in a common region between ADL0198 (chr 1: 171.7 Mb) and ABR0287 (chr 1: 173.4 Mb) on chicken chromosome 1 in an F2 resource population produced by crossing low- and high-Growth lines of the Hinai-dori breed. Motilin receptor (MLNR) is a candidate gene affecting Growth Traits in the region. In this study, we genotyped polymorphisms of the MLNR gene and investigated its association with Growth Traits in a Hinai-dori F2 intercross population. All the exons of the MLNR gene in the parental population were subjected to PCR amplification, nucleotide sequenced and haplotypes identified. To distinguish resultant diplotype individuals in the F2 population, a mismatch amplification mutation assay was performed. Three haplotypes (Haplotypes 1–3) were accordingly identified. Six genotypes produced by the combination of three haplotypes (Haplotype 1, 2, and 3) were examined in order to identify associations between MLNR haplotypes and Growth Traits. The data showed that Haplotype 1 was superior to Haplotype 2 and 3 in body weight at 10 and 14 weeks of age, average daily gain between 4 and 10 weeks, 10 and 14 weeks, and 0 and 14 weeks of age in female in F2 females. It was concluded that MLNR is a useful marker of Growth Traits and could be used to develop strategies for improving Growth Traits in the Hinai-dori breed.
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association between cholecystokinin type a receptor haplotypes and Growth Traits in japanese hinai dori crossbred chickens
Molecular Biology Reports, 2012Co-Authors: Kazuhiro Rikimaru, Megumi Komatsu, Yoshinobu Uemoto, Keiichi Suzuki, Hisato Takeda, Hideaki TakahashiAbstract:We previously identified quantitative trait loci for body weight and average daily gain in a common region between MCW0240 (chr 4: 69.9 Mb) and ABR0622 (chr 4: 86.3 Mb) on chicken chromosome 4 in an F2 resource population produced by crossing low- and high-Growth lines of the Hinai-dori breed. Cholecystokinin type A receptor (CCKAR) is a candidate gene affecting Growth Traits in the region. In this study, we genotyped polymorphisms of the CCKAR gene and investigated its association with Growth Traits in a Hinai-dori F2 intercross population. All the exons of the CCKAR gene in the parental population were subjected to PCR amplification, nucleotide sequenced and haplotypes identified. To distinguish resultant diplotype individuals in the F2 population, a mismatch amplification mutation assay was performed. Five haplotypes (Haplotypes 1–5) were accordingly identified. Six genotypes produced by the combination of three haplotypes (Haplotype 1, 3, and 4) were examined in order to identify associations between CCKAR haplotypes and Growth Traits. The data indicate that Haplotype 1 was superior to Haplotype 3 and 4 in body weight at 10 and 14 weeks of age, average daily gain between 4 and 10 weeks, 10 and 14 weeks, and 0 and 14 weeks of age. It was concluded that CCKAR is a useful marker of Growth Traits and could be used to develop strategies for improving Growth Traits in the Hinai-dori breed.
Hong Chen - One of the best experts on this subject based on the ideXlab platform.
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Detection of 15-bp Deletion Mutation within PLAG1 Gene and Its Effects on Growth Traits in Goats
'MDPI AG', 2021Co-Authors: Zhenyu Wei, Hong Chen, Chuanying Pan, Ke Wang, Zhen Wang, Xianyong LanAbstract:Stature and weight are important Growth and development Traits for animals, which also significantly affect the productivity of livestock. Polymorphic adenoma gene 1 (PLAG1) is located in the Growth-related quantitative trait nucleotides (QTN), and its variation has been determined to significantly affect the body stature of bovines. This study found that novel 15-bp InDel could significantly influence important Growth Traits in goats. The frequencies of genotypes of the 15-bp mutation and relationship with core Growth Traits such as body weight, body height, height at hip cross, chest circumference, hip width and body index were explored in 1581 individuals among 4 Chinese native goat breeds. The most frequent genotypes of Shaanbei white Cashmere goat (SWCG), Inner Mongolia White Cashmere goat (IMCG) and Guanzhong Dairy goat (GZDG) were II genotypes (insertion/insertion), and the frequency of ID genotype (insertion/deletion) was found to be slightly higher than that of II genotype in Hainan Black goat (HNBG), showing that the frequency of the I allele was higher than that of the D allele. In adult goats, there were significant differences between 15-bp variation and body weight, chest circumference and body height Traits in SWCG (p p = 0.044) and hip width in GZDG (p = 0.002). In regard to lambs, there were significant differences in height at the hip cross of SWCG (p = 0.036) and hip width in IMWC (p = 0.005). The corresponding results suggest that the 15-bp InDel mutation of PLAG1 is associated with the regulation of important Growth characteristics of both adult and lamb of goats, which may serve as efficient molecular markers for goat breeding
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tetra primer amplification refractory mutation system pcr t arms pcr rapidly identified a critical missense mutation p236t of bovine acadvl gene affecting Growth Traits
Gene, 2015Co-Authors: Sihuan Zhang, Yonglong Dang, Qingfeng Zhang, Qiaomei Qin, Chuzhao Lei, Hong Chen, Xianyong LanAbstract:Acyl-CoA dehydrogenase, very long chain (ACADVL), encoding ACADVL protein, targets the inner mitochondrial membrane where it catalyzes the first step of the mitochondrial fatty acid beta-oxidation pathway and plays an important role in body metabolism and oxidation of long chain fatty acid releasing energy. Tetra-primer amplification refractory mutation system PCR (T-ARMS-PCR) is an easy-to-operate, rapid, inexpensive, and exact method for SNP genotyping. Herein, T-ARMS-PCR was carried out to detect a critical missense mutation (AC_000176:g.2885C>A; Pro236Thr) within the ACADVL gene in 644 individuals from two cattle breeds. In order to evaluate the accuracy of the T-ARMS-PCR at this locus, the genotype of the sampled individuals was also identified by PCR-RFLP. The concordance between these two methods was 98.76%. Statistical analysis showed that the bovine ACADVL gene had a significant effect on chest width (P < 0.05), chest depth (P < 0.05), and hip width (P < 0.05) in the Qinchuan breed. The cattle with AA genotype had superior Growth Traits compared to cattle with AC and/or CC genotypes. The “A” allele had positive effects on Growth Traits. Therefore, T-ARMS-PCR can replace PCR-RFLP for rapid genotyping of this mutation, which could be used as a DNA marker for selecting individuals with superior Growth Traits in the Qinchuan breed. These findings contribute to breeding and genetics in beef cattle industry.
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A critical functional missense mutation (H173R) in the bovine PROP1 gene significantly affects Growth Traits in cattle.
Gene, 2013Co-Authors: Chuanying Pan, Chuzhao Lei, Xianyong Lan, Hu Shenrong, Wenchao Jia, Hong ChenAbstract:Abstract The PROP1 protein, encoded by the prophet of Pit-1 (PROP1) gene, exhibits both DNA-binding and transcriptional activation abilities. Its expression leads to the ontogenesis of Growth hormone (GH), prolactin (PRL), thyroid-stimulating hormone (TSH), and pituitary hormone. The missense mutation H173R in PROP1 may result in deficiencies of GH, PRL, TSH, and Pit-1, thereby affecting Growth Traits. The objective of this study was to characterize the H173R mutation within the PROP1 gene and examine its associations with Growth Traits in cattle. Accordingly, the H173R mutation was genotyped in 1207 cows belonging to five Chinese native breeds. Three genotypes were identified among the specimens, with genotype AA being the major one. Consequently, the “G” allele was the minor allele. Association testing revealed that the H173R mutation was significantly associated with body weight, average daily weight gain and physical parameters in the analyzed breeds. Interestingly, the cows with genotype AG and/or AA had superior Growth Traits compared with those expressing the GG genotype, in all tested breeds. These findings revealed that the “A” allele had positive effects on Growth Traits, which was consistent with the increasing binding ability and enhanced activation capacity associated with the bovine isoform PROP1-173H, representing the “A” allele. Therefore, the H173R mutation can be considered as a DNA marker for selecting individuals with superior Growth Traits, thereby contributing to research on breeding and genetics in the beef industry.
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haplotypes and effects on Growth Traits of bovine wnt7a gene in chinese qinchuan cattle
Gene, 2013Co-Authors: Jing Xue, Chuzhao Lei, Xianyong Lan, Chunlei Zhang, Yujia Sun, Wenjiao Guo, Ziqi Yang, Huibin Tian, Hong ChenAbstract:Wnt7a is a member of the WNT gene family, which encodes secreted signaling proteins and responds to many biological processes. Specifically Wnt7a influences satellite stem cells and regulates the regenerative potential of the muscle. However, similar researches about the bovine Wnt7a gene are lacking. Therefore, in this study, polymorphisms of the bovine Wnt7a gene were detected in 488 individuals from Chinese Qinchuan cattle by DNA pooling, forced PCR-RFLP, and DNA sequencing methods. 3 novel SNPs were identified, two SNPs (g.T4926C and g.A21943G) were in the intron and the last one (g.C63777T) was in the exon. Five haplotypes involved in these three variant sites in the Wnt7a gene were identified and their effects on Growth Traits were analyzed. The results revealed that haplotype 1 had the highest haplotype frequencies and was highly significantly associated with body height (P<0.01), body weight (P<0.05), chest width (P<0.05) and height at hip cross (P<0.01) respectively.
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mrna expression pattern and association study with Growth Traits of bovine vaspin gene
Molecular Biology Reports, 2013Co-Authors: Xinsheng Lai, Chuzhao Lei, Xianyong Lan, Jing Wang, Chenge Zhang, Chen Wang, Chenlei Zhang, Hong ChenAbstract:Visceral adipose tissue-derived serine protease inhibitor (vaspin) is an interesting novel adipocytokine with insulin-sensitizing effects. Some studies have suggested that vaspin could play an important role in the development of obesity and metabolic disorders. However, the tissue expression patterns in cattle and impact of vaspin gene variants on the Growth Traits has not been determined yet. Herein, we firstly investigated the tissue expression patterns of vaspin gene in new born and adult cattle. The results showed that vaspin was ubiquitously expressed in most tissues and strongly expressed in the heart, skeletal muscle and fat. Then, genetic variants within bovine vaspin gene were screened in 1235 individuals from five Chinese indigenous cattle breeds. Two novel mutations in coding region (NW_001494061: g.1124477 G>A and g.1118561 T>C) of bovine vaspin gene were identified using MspI PCR–RFLP and HhaI ACRS PCR–RFLP detection. Association analysis revealed both two mutations were significantly associated with bodyweight and chest girth at 24 months in cattle (P < 0.05). Therefore, the MspI and HhaI genetic variants of bovine vaspin gene were recommended as DNA markers related to Growth Traits through marker-assisted selection for genetics and breeding in cattle.
Chuzhao Lei - One of the best experts on this subject based on the ideXlab platform.
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The evaluation of 23-bp and 12-bp insertion/deletion within the PRNP gene and their effects on Growth Traits in healthy Chinese native cattle breeds
Taylor & Francis Group, 2018Co-Authors: Qing Yang, Sihuan Zhang, Chuzhao Lei, Liangliang Liu, Fengpeng Lin, Jiming Liu, Rongmin WangAbstract:Polymorphisms in the prion protein gene (PRNP) have been linked with the occurrence of transmissible spongiform encephalopathies and the expression of some phenotypic Traits in healthy animals including cattle. The aim of this study was to verify the 23-bp insertion/deletion (indel) within promoter region and the 12-bp indel within intron 1 of PRNP, as well as to evaluate their associations with Growth Traits. Two loci of PRNP were genotyped in 1558 healthy Chinese cattle sampled from 6 indigenous breeds. Moreover, phenotypic records for Growth were used to find the relationship between PRNP polymorphisms and phenotypic performance. The results confirmed the occurrence of these two indel polymorphisms in the analysed breeds. Association analysis showed that the 23-bp indel was significantly related to the body length and heart girth in 18-months-old Nanyang cattle. The 12-bp indel was significantly related to the Growth Traits in three cattle breeds, such as the body weight in Xia'nan cattle, the daily gained weight in 12-months-old Nanyang cattle and the cannon circumference and rump length in Ji'an cattle. These findings indicated that these two indels may affect bovine Growth Traits, which could benefit to healthy cattle selection and breeding through marker-assisted selection. Abbreviations AI: artificial insemination; ANOVA: analysis of variance; Bp: base pair; BSE: bovine spongiform encephalopathy; D: deletion; DD: deletion/deletion; GLM: generalized linear model; reaction; He: heterozygosity; Ho: homozygosity; HWE: Hardy–Weinberg equilibrium; I: insertion; ID: insertion/deletion; II: insertion/insertion; Indel: insertion/deletion; MAS: marker-assisted selection; Ne: effective allele numbers; PCR: polymerase chain reaction; PIC: polymorphism information content; PrP: Prion protein (PrP); PRNP: the prion protein; RP58: repressor protein with a predicted molecular mass of 58 kDa; SNP: single nucleotide polymorphism; Sp1: specific protein 1; SPSS: statistical product and service solutions; TSE: transmissible spongiform encephalopathie
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tetra primer amplification refractory mutation system pcr t arms pcr rapidly identified a critical missense mutation p236t of bovine acadvl gene affecting Growth Traits
Gene, 2015Co-Authors: Sihuan Zhang, Yonglong Dang, Qingfeng Zhang, Qiaomei Qin, Chuzhao Lei, Hong Chen, Xianyong LanAbstract:Acyl-CoA dehydrogenase, very long chain (ACADVL), encoding ACADVL protein, targets the inner mitochondrial membrane where it catalyzes the first step of the mitochondrial fatty acid beta-oxidation pathway and plays an important role in body metabolism and oxidation of long chain fatty acid releasing energy. Tetra-primer amplification refractory mutation system PCR (T-ARMS-PCR) is an easy-to-operate, rapid, inexpensive, and exact method for SNP genotyping. Herein, T-ARMS-PCR was carried out to detect a critical missense mutation (AC_000176:g.2885C>A; Pro236Thr) within the ACADVL gene in 644 individuals from two cattle breeds. In order to evaluate the accuracy of the T-ARMS-PCR at this locus, the genotype of the sampled individuals was also identified by PCR-RFLP. The concordance between these two methods was 98.76%. Statistical analysis showed that the bovine ACADVL gene had a significant effect on chest width (P < 0.05), chest depth (P < 0.05), and hip width (P < 0.05) in the Qinchuan breed. The cattle with AA genotype had superior Growth Traits compared to cattle with AC and/or CC genotypes. The “A” allele had positive effects on Growth Traits. Therefore, T-ARMS-PCR can replace PCR-RFLP for rapid genotyping of this mutation, which could be used as a DNA marker for selecting individuals with superior Growth Traits in the Qinchuan breed. These findings contribute to breeding and genetics in beef cattle industry.
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A critical functional missense mutation (H173R) in the bovine PROP1 gene significantly affects Growth Traits in cattle.
Gene, 2013Co-Authors: Chuanying Pan, Chuzhao Lei, Xianyong Lan, Hu Shenrong, Wenchao Jia, Hong ChenAbstract:Abstract The PROP1 protein, encoded by the prophet of Pit-1 (PROP1) gene, exhibits both DNA-binding and transcriptional activation abilities. Its expression leads to the ontogenesis of Growth hormone (GH), prolactin (PRL), thyroid-stimulating hormone (TSH), and pituitary hormone. The missense mutation H173R in PROP1 may result in deficiencies of GH, PRL, TSH, and Pit-1, thereby affecting Growth Traits. The objective of this study was to characterize the H173R mutation within the PROP1 gene and examine its associations with Growth Traits in cattle. Accordingly, the H173R mutation was genotyped in 1207 cows belonging to five Chinese native breeds. Three genotypes were identified among the specimens, with genotype AA being the major one. Consequently, the “G” allele was the minor allele. Association testing revealed that the H173R mutation was significantly associated with body weight, average daily weight gain and physical parameters in the analyzed breeds. Interestingly, the cows with genotype AG and/or AA had superior Growth Traits compared with those expressing the GG genotype, in all tested breeds. These findings revealed that the “A” allele had positive effects on Growth Traits, which was consistent with the increasing binding ability and enhanced activation capacity associated with the bovine isoform PROP1-173H, representing the “A” allele. Therefore, the H173R mutation can be considered as a DNA marker for selecting individuals with superior Growth Traits, thereby contributing to research on breeding and genetics in the beef industry.
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haplotypes and effects on Growth Traits of bovine wnt7a gene in chinese qinchuan cattle
Gene, 2013Co-Authors: Jing Xue, Chuzhao Lei, Xianyong Lan, Chunlei Zhang, Yujia Sun, Wenjiao Guo, Ziqi Yang, Huibin Tian, Hong ChenAbstract:Wnt7a is a member of the WNT gene family, which encodes secreted signaling proteins and responds to many biological processes. Specifically Wnt7a influences satellite stem cells and regulates the regenerative potential of the muscle. However, similar researches about the bovine Wnt7a gene are lacking. Therefore, in this study, polymorphisms of the bovine Wnt7a gene were detected in 488 individuals from Chinese Qinchuan cattle by DNA pooling, forced PCR-RFLP, and DNA sequencing methods. 3 novel SNPs were identified, two SNPs (g.T4926C and g.A21943G) were in the intron and the last one (g.C63777T) was in the exon. Five haplotypes involved in these three variant sites in the Wnt7a gene were identified and their effects on Growth Traits were analyzed. The results revealed that haplotype 1 had the highest haplotype frequencies and was highly significantly associated with body height (P<0.01), body weight (P<0.05), chest width (P<0.05) and height at hip cross (P<0.01) respectively.
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mrna expression pattern and association study with Growth Traits of bovine vaspin gene
Molecular Biology Reports, 2013Co-Authors: Xinsheng Lai, Chuzhao Lei, Xianyong Lan, Jing Wang, Chenge Zhang, Chen Wang, Chenlei Zhang, Hong ChenAbstract:Visceral adipose tissue-derived serine protease inhibitor (vaspin) is an interesting novel adipocytokine with insulin-sensitizing effects. Some studies have suggested that vaspin could play an important role in the development of obesity and metabolic disorders. However, the tissue expression patterns in cattle and impact of vaspin gene variants on the Growth Traits has not been determined yet. Herein, we firstly investigated the tissue expression patterns of vaspin gene in new born and adult cattle. The results showed that vaspin was ubiquitously expressed in most tissues and strongly expressed in the heart, skeletal muscle and fat. Then, genetic variants within bovine vaspin gene were screened in 1235 individuals from five Chinese indigenous cattle breeds. Two novel mutations in coding region (NW_001494061: g.1124477 G>A and g.1118561 T>C) of bovine vaspin gene were identified using MspI PCR–RFLP and HhaI ACRS PCR–RFLP detection. Association analysis revealed both two mutations were significantly associated with bodyweight and chest girth at 24 months in cattle (P < 0.05). Therefore, the MspI and HhaI genetic variants of bovine vaspin gene were recommended as DNA markers related to Growth Traits through marker-assisted selection for genetics and breeding in cattle.
Kazuhiro Rikimaru - One of the best experts on this subject based on the ideXlab platform.
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association between motilin receptor gene haplotypes and Growth Traits in japanese hinai dori crossbred chickens
Asian-australasian Journal of Animal Sciences, 2014Co-Authors: Hideaki Takahashi, Kazuhiro Rikimaru, Megumi Komatsu, Yoshinobu Uemoto, Keiichi SuzukiAbstract:We previously identified quantitative trait loci (QTL) for body weight and average daily gain in a common region between ADL0198 (chr 1: 171.7 Mb) and ABR0287 (chr 1: 173.4 Mb) on chicken chromosome 1 in an F2 resource population produced by crossing low- and high-Growth lines of the Hinai-dori breed. Motilin receptor (MLNR) is a candidate gene affecting Growth Traits in the region. In this study, we genotyped polymorphisms of the MLNR gene and investigated its association with Growth Traits in a Hinai-dori F2 intercross population. All the exons of the MLNR gene in the parental population were subjected to PCR amplification, nucleotide sequenced and haplotypes identified. To distinguish resultant diplotype individuals in the F2 population, a mismatch amplification mutation assay was performed. Three haplotypes (Haplotypes 1–3) were accordingly identified. Six genotypes produced by the combination of three haplotypes (Haplotype 1, 2, and 3) were examined in order to identify associations between MLNR haplotypes and Growth Traits. The data showed that Haplotype 1 was superior to Haplotype 2 and 3 in body weight at 10 and 14 weeks of age, average daily gain between 4 and 10 weeks, 10 and 14 weeks, and 0 and 14 weeks of age in female in F2 females. It was concluded that MLNR is a useful marker of Growth Traits and could be used to develop strategies for improving Growth Traits in the Hinai-dori breed.
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association between cholecystokinin type a receptor haplotypes and Growth Traits in japanese hinai dori crossbred chickens
Molecular Biology Reports, 2012Co-Authors: Kazuhiro Rikimaru, Megumi Komatsu, Yoshinobu Uemoto, Keiichi Suzuki, Hisato Takeda, Hideaki TakahashiAbstract:We previously identified quantitative trait loci for body weight and average daily gain in a common region between MCW0240 (chr 4: 69.9 Mb) and ABR0622 (chr 4: 86.3 Mb) on chicken chromosome 4 in an F2 resource population produced by crossing low- and high-Growth lines of the Hinai-dori breed. Cholecystokinin type A receptor (CCKAR) is a candidate gene affecting Growth Traits in the region. In this study, we genotyped polymorphisms of the CCKAR gene and investigated its association with Growth Traits in a Hinai-dori F2 intercross population. All the exons of the CCKAR gene in the parental population were subjected to PCR amplification, nucleotide sequenced and haplotypes identified. To distinguish resultant diplotype individuals in the F2 population, a mismatch amplification mutation assay was performed. Five haplotypes (Haplotypes 1–5) were accordingly identified. Six genotypes produced by the combination of three haplotypes (Haplotype 1, 3, and 4) were examined in order to identify associations between CCKAR haplotypes and Growth Traits. The data indicate that Haplotype 1 was superior to Haplotype 3 and 4 in body weight at 10 and 14 weeks of age, average daily gain between 4 and 10 weeks, 10 and 14 weeks, and 0 and 14 weeks of age. It was concluded that CCKAR is a useful marker of Growth Traits and could be used to develop strategies for improving Growth Traits in the Hinai-dori breed.
Xianyong Lan - One of the best experts on this subject based on the ideXlab platform.
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Detection of 15-bp Deletion Mutation within PLAG1 Gene and Its Effects on Growth Traits in Goats
'MDPI AG', 2021Co-Authors: Zhenyu Wei, Hong Chen, Chuanying Pan, Ke Wang, Zhen Wang, Xianyong LanAbstract:Stature and weight are important Growth and development Traits for animals, which also significantly affect the productivity of livestock. Polymorphic adenoma gene 1 (PLAG1) is located in the Growth-related quantitative trait nucleotides (QTN), and its variation has been determined to significantly affect the body stature of bovines. This study found that novel 15-bp InDel could significantly influence important Growth Traits in goats. The frequencies of genotypes of the 15-bp mutation and relationship with core Growth Traits such as body weight, body height, height at hip cross, chest circumference, hip width and body index were explored in 1581 individuals among 4 Chinese native goat breeds. The most frequent genotypes of Shaanbei white Cashmere goat (SWCG), Inner Mongolia White Cashmere goat (IMCG) and Guanzhong Dairy goat (GZDG) were II genotypes (insertion/insertion), and the frequency of ID genotype (insertion/deletion) was found to be slightly higher than that of II genotype in Hainan Black goat (HNBG), showing that the frequency of the I allele was higher than that of the D allele. In adult goats, there were significant differences between 15-bp variation and body weight, chest circumference and body height Traits in SWCG (p p = 0.044) and hip width in GZDG (p = 0.002). In regard to lambs, there were significant differences in height at the hip cross of SWCG (p = 0.036) and hip width in IMWC (p = 0.005). The corresponding results suggest that the 15-bp InDel mutation of PLAG1 is associated with the regulation of important Growth characteristics of both adult and lamb of goats, which may serve as efficient molecular markers for goat breeding
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Exploration of Genetic Variants within the Goat A-Kinase Anchoring Protein 12 (AKAP12) Gene and Their Effects on Growth Traits
'MDPI AG', 2021Co-Authors: Yangyang Bai, Xianyong Lan, Zihong Kang, Rongrong Yuan, Yunyun Luo, Haijing Zhu, Xiaoyue SongAbstract:The A-kinase anchoring protein 12 gene (AKAP12) is a scaffold protein, which can target multiple signal transduction effectors, can promote mitosis and cytokinesis and plays an important role in the regulation of Growth and development. In our previous study, P1–7 bp (intron 3) and P2–13 bp (3′UTR) indels within the AKAP12 gene significantly influenced AKAP12 gene expression. Therefore, this study aimed to identify the association between these two genetic variations and Growth-related Traits in Shaanbei white cashmere goats (SBWC) (n = 1405). Herein, we identified two non-linkage insertions/deletions (indels). Notably, we found that the P1–7 bp indel mutation was related to the height at hip cross (HHC; p p AKAP12 affected Growth Traits in goats. Compared to the P1–7 bp indel, the P2–13 bp indel is more suitable for the breeding of goat Growth Traits
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tetra primer amplification refractory mutation system pcr t arms pcr rapidly identified a critical missense mutation p236t of bovine acadvl gene affecting Growth Traits
Gene, 2015Co-Authors: Sihuan Zhang, Yonglong Dang, Qingfeng Zhang, Qiaomei Qin, Chuzhao Lei, Hong Chen, Xianyong LanAbstract:Acyl-CoA dehydrogenase, very long chain (ACADVL), encoding ACADVL protein, targets the inner mitochondrial membrane where it catalyzes the first step of the mitochondrial fatty acid beta-oxidation pathway and plays an important role in body metabolism and oxidation of long chain fatty acid releasing energy. Tetra-primer amplification refractory mutation system PCR (T-ARMS-PCR) is an easy-to-operate, rapid, inexpensive, and exact method for SNP genotyping. Herein, T-ARMS-PCR was carried out to detect a critical missense mutation (AC_000176:g.2885C>A; Pro236Thr) within the ACADVL gene in 644 individuals from two cattle breeds. In order to evaluate the accuracy of the T-ARMS-PCR at this locus, the genotype of the sampled individuals was also identified by PCR-RFLP. The concordance between these two methods was 98.76%. Statistical analysis showed that the bovine ACADVL gene had a significant effect on chest width (P < 0.05), chest depth (P < 0.05), and hip width (P < 0.05) in the Qinchuan breed. The cattle with AA genotype had superior Growth Traits compared to cattle with AC and/or CC genotypes. The “A” allele had positive effects on Growth Traits. Therefore, T-ARMS-PCR can replace PCR-RFLP for rapid genotyping of this mutation, which could be used as a DNA marker for selecting individuals with superior Growth Traits in the Qinchuan breed. These findings contribute to breeding and genetics in beef cattle industry.
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A critical functional missense mutation (H173R) in the bovine PROP1 gene significantly affects Growth Traits in cattle.
Gene, 2013Co-Authors: Chuanying Pan, Chuzhao Lei, Xianyong Lan, Hu Shenrong, Wenchao Jia, Hong ChenAbstract:Abstract The PROP1 protein, encoded by the prophet of Pit-1 (PROP1) gene, exhibits both DNA-binding and transcriptional activation abilities. Its expression leads to the ontogenesis of Growth hormone (GH), prolactin (PRL), thyroid-stimulating hormone (TSH), and pituitary hormone. The missense mutation H173R in PROP1 may result in deficiencies of GH, PRL, TSH, and Pit-1, thereby affecting Growth Traits. The objective of this study was to characterize the H173R mutation within the PROP1 gene and examine its associations with Growth Traits in cattle. Accordingly, the H173R mutation was genotyped in 1207 cows belonging to five Chinese native breeds. Three genotypes were identified among the specimens, with genotype AA being the major one. Consequently, the “G” allele was the minor allele. Association testing revealed that the H173R mutation was significantly associated with body weight, average daily weight gain and physical parameters in the analyzed breeds. Interestingly, the cows with genotype AG and/or AA had superior Growth Traits compared with those expressing the GG genotype, in all tested breeds. These findings revealed that the “A” allele had positive effects on Growth Traits, which was consistent with the increasing binding ability and enhanced activation capacity associated with the bovine isoform PROP1-173H, representing the “A” allele. Therefore, the H173R mutation can be considered as a DNA marker for selecting individuals with superior Growth Traits, thereby contributing to research on breeding and genetics in the beef industry.
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haplotypes and effects on Growth Traits of bovine wnt7a gene in chinese qinchuan cattle
Gene, 2013Co-Authors: Jing Xue, Chuzhao Lei, Xianyong Lan, Chunlei Zhang, Yujia Sun, Wenjiao Guo, Ziqi Yang, Huibin Tian, Hong ChenAbstract:Wnt7a is a member of the WNT gene family, which encodes secreted signaling proteins and responds to many biological processes. Specifically Wnt7a influences satellite stem cells and regulates the regenerative potential of the muscle. However, similar researches about the bovine Wnt7a gene are lacking. Therefore, in this study, polymorphisms of the bovine Wnt7a gene were detected in 488 individuals from Chinese Qinchuan cattle by DNA pooling, forced PCR-RFLP, and DNA sequencing methods. 3 novel SNPs were identified, two SNPs (g.T4926C and g.A21943G) were in the intron and the last one (g.C63777T) was in the exon. Five haplotypes involved in these three variant sites in the Wnt7a gene were identified and their effects on Growth Traits were analyzed. The results revealed that haplotype 1 had the highest haplotype frequencies and was highly significantly associated with body height (P<0.01), body weight (P<0.05), chest width (P<0.05) and height at hip cross (P<0.01) respectively.
