The Experts below are selected from a list of 87 Experts worldwide ranked by ideXlab platform
Thomas E. Finger - One of the best experts on this subject based on the ideXlab platform.
-
5 ht3a driven green fluorescent protein delineates Gustatory fibers innervating sour responsive taste cells a labeled line for sour taste
The Journal of Comparative Neurology, 2017Co-Authors: Jennifer M Stratford, Ruibiao Yang, Eric D Larson, Ernesto E Salcedo, Thomas E. FingerAbstract:Taste buds contain multiple cell types with each type expressing receptors and transduction components for a subset of taste qualities. The sour sensing cells, Type III cells, release serotonin (5-HT) in response to the presence of sour (acidic) tastants and this released 5-HT activates 5-HT3 receptors on the Gustatory nerves. We show here, using 5-HT3A GFP mice, that 5-HT3 -expressing nerve fibers preferentially contact and receive synaptic contact from Type III taste cells. Further, these 5-HT3 -expressing nerve fibers terminate in a restricted central-lateral portion of the Nucleus of the solitary tract (nTS)-the same area that shows increased c-Fos expression upon presentation of a sour tastant (30 mM citric acid). This acid stimulation also evokes c-Fos in the laterally adjacent mediodorsal spinal trigeminal Nucleus (DMSp5), but this trigeminal activation is not associated with the presence of 5-HT3 -expressing nerve fibers as it is in the nTS. Rather, the neuronal activation in the trigeminal complex likely is attributable to direct depolarization of acid-sensitive trigeminal nerve fibers, for example, polymodal nociceptors, rather than through taste buds. Taken together, these findings suggest that transmission of sour taste information involves communication between Type III taste cells and 5-HT3 -expressing afferent nerve fibers that project to a restricted portion of the nTS consistent with a crude mapping of taste quality information in the primary Gustatory Nucleus.
-
co occurrence of calcium binding proteins and calcium permeable glutamate receptors in the primary Gustatory Nucleus of goldfish
The Journal of Comparative Neurology, 2006Co-Authors: Takanori Ikenaga, Thomas E. Finger, Gema HuesaAbstract:Primary vagal Gustatory afferents utilize glutamate as a neurotransmitter acting on AMPA/kainate receptors of second-order neurons. Some forms of ionotropic glutamate receptors permit passage of Ca++ ions upon activation by appropriate ligands. Calcium-binding proteins (CaBPs) play a buffering role for regulating the concentration of intracellular calcium. In the present study, we used immunohistochemistry to examine the distribution and morphology of neurons with CaBPs, including calretinin, calbindin, and parvalbumin, and to compare this distribution with neurons exhibiting Ca++-permeable glutamate receptors as determined by kainate-stimulated uptake of Co++ in the vagal lobe of goldfish. Calretinin- and calbindin-positive neurons occurred throughout the sensory zone including round unipolar, horizontal; and perpendicular bipolar or multipolar somata. Parvalbumin neurons were mainly round monopolar neurons, especially common in the superficial layers of the sensory zone. In the motor zone, while parvalbumin labeled nearly all motoneurons, calretinin labeled only external motoneurons. In double labeling with calretinin and parvalbumin, few neurons in the sensory layer labeled with both antisera. Immunocytochemistry following kainate-stimulate uptake of Co++ showed that most calretinin, but few parvalbumin immunopositive neurons also were labeled by cobalt in the central and deep layers of the sensory zone. All motoneurons were labeled by Co++, including those immunoreactive for calretinin or parvalbumin. These results indicate that calretinin expression is strongly correlated with calcium-permeable ionotropic glutamate receptors in the neurons of the sensory zone of the goldfish vagal lobe, but even within this limited region, not all Ca++-permeable neurons possess any of the CaBPs examined. J. Comp. Neurol. 499:90–105, 2006. © 2006 Wiley-Liss, Inc.
-
kainate activated cobalt uptake in the primary Gustatory Nucleus in goldfish visualization of the morphology and distribution of cells expressing ampa kainate receptors in the vagal lobe
The Journal of Comparative Neurology, 2001Co-Authors: Barbel Bottger, Cynthia A Smeraski, Thomas E. FingerAbstract:Gustatory afferent fibers of the vagus nerve that innervate taste buds of the oropharynx of the goldfish, Carassius auratus, project to the vagal lobe, which is a laminated Gustatory Nucleus in the dorsal medulla. As in the mammalian Gustatory system, responses by second-order cells in the goldfish medulla are mediated by N-methyl-D-aspartate (NMDA) and non-NMDA ionotropic glutamate receptors. We utilized a cobalt uptake technique to label vagal lobe neurons that possess cobalt-permeable ionotropic glutamate receptors. Vagal lobe slices were bathed in kainate (40 μM) or glutamate (0.5 or 1 mM) in the presence of CoCl2, which can pass into cells through the ligand-gated cation channels of non-NMDA receptors made up of certain subunit combinations. Cobalt-filled cells and dendrites were observed in slices that were activated by kainate or glutamate, but not in control slices that were bathed in CoCl2 alone, nor in slices that were bathed with the non-NMDA receptor antagonist 6,7-dinitroquinoxaline-2,3-dione (10 μM) in addition to an agonist. Likewise, simple depolarization of the cells with KCl failed to induce cobalt loading. Cobalt-filled round unipolar cells, elongate or globular bipolar cells, and multipolar cells with elongate or polygonal perikarya were distributed throughout the cell layers in the sensory zone of the vagal lobe. Numerous labeled neurons had dendrites spanning layers IV and VI, the two principal layers of primary afferent input. Apical and basal dendrites often extended radially through neighboring laminae, but many cells also extended dendrites tangential to the lamination of the sensory zone. In the motor layer, cell bodies and proximal dendrites of small, multipolar neurons, and large motoneurons were regularly loaded with cobalt. J. Comp. Neurol. 431:59–74, 2001. © 2001 Wiley-Liss, Inc.
-
excitatory amino acid neurotransmission in the primary Gustatory Nucleus of the goldfish carassius auratus
Annals of the New York Academy of Sciences, 1998Co-Authors: Cynthia A Smeraski, Thomas V Dunwiddie, Lihong Diao, Thomas E. FingerAbstract:The vagal lobe in goldfish is a laminated structure in the midmedulla responsible for processing vagal Gustatory input from the oropharynx. The anatomical arrangement of the vagal lobe is conducive to an in vitro slice preparation for investigating the physiology and pharmacology of primary Gustatory fibers. Postsynaptic population responses (N2 and N3) were evoked from sensory layers of the vagal lobe following stimulation of the incoming vagal fibers. Application of 100 microM kynurenic acid, a broad spectrum glutamate receptor antagonist, abolished or significantly decreased the evoked responses. These results indicate that excitatory amino acids are the neurotransmitter at the first relay in the taste pathway in the central nervous system.
-
nmda and non nmda receptors mediate responses in the primary Gustatory Nucleus in goldfish
Chemical Senses, 1998Co-Authors: Cynthia A Smeraski, Thomas V Dunwiddie, Lihong Diao, Thomas E. FingerAbstract:Primary Gustatory afferents from the oropharynx of the goldfish, Carassius auratus, terminate in the vagal lobe, a laminated structure in the dorsal medulla comparable to the Gustatory portion of the Nucleus of the solitary tract in mammals. We utilized an in vitro brain slice preparation to test the role of different ionotropic glutamate receptor subtypes in synaptic transmission of Gustatory information by recording changes in field potentials after application of various glutamate receptor antagonists. Electrical stimulation of the vagus nerve (NX) evokes two short-latency postsynaptic field potentials from sensory layers of the vagal lobe. 6,7-Dinitroquinoxaline-2,3-dione and 6-nitro-7-sulphamoylbenzo[f]quinoxaline-2,3-dione, two non-N-methyl-D-aspartate (NMDA) ionotropic receptor antagonists, blocked these short-latency potentials. Slower potentials that were revealed under Mg 2+ -free conditions, were abolished by the NMDA receptor antagonist, D(-)-2-amino-5-phosphonovaleric acid (APV). Repetitive stimulation produced short-term facilitation, which was attenuated by application of APV. These results indicate that the synaptic responses in the vagal lobe produced by stimulation of the Gustatory roots of the NX involve both NMDA and non-NMDA receptors. An NMDA receptor-mediated facilitation may serve to amplify incoming bursts of primary afferent activity.
Julian Yanez - One of the best experts on this subject based on the ideXlab platform.
-
Gustatory and general visceral centers and their connections in the brain of adult zebrafish a carbocyanine dye tract tracing study
The Journal of Comparative Neurology, 2017Co-Authors: Julian Yanez, Monica Folgueira, Yara Souto, Laura Pineiro, Ramon AnadonAbstract:The central connections of the Gustatory/general visceral system of the adult zebrafish (Danio rerio) were examined by means of carbocyanine dye tracing. Main primary Gustatory centers (facial and vagal lobes) received sensory projections from the facial and vagal nerves, respectively. The vagal nerve also projects to the commissural Nucleus of Cajal, a general visceral sensory center. These primary centers mainly project on a prominent secondary Gustatory and general visceral Nucleus (SGN/V) located in the isthmic region. Secondary projections on the SGN/V were topographically organized, those of the facial lobe mainly ending medially to those of the vagal lobe, and those from the commissural Nucleus ventrolaterally. Descending facial lobe projections to the medial funicular Nucleus were also noted. Ascending fibers originating from the SGN/V mainly projected to the posterior thalamic Nucleus and the lateral hypothalamus (lateral torus, lateral recess Nucleus, hypothalamic inferior lobe diffuse Nucleus) and an intermediate cell- and fiber-rich region termed here the tertiary Gustatory Nucleus proper, but not to a Nucleus formerly considered as the zebrafish tertiary Gustatory Nucleus. The posterior thalamic Nucleus, tertiary Gustatory Nucleus proper, and Nucleus of the lateral recess gave rise to descending projections to the SGN/V and the vagal lobe. The connectivity between diencephalic Gustatory centers and the telencephalon was also investigated. The present results showed that the Gustatory connections of the adult zebrafish are rather similar to those reported in other cyprinids, excepting the tertiary Gustatory Nucleus. Similarities between the Gustatory systems of zebrafish and other fishes are also discussed. J. Comp. Neurol. 525:333-362, 2017. © 2016 Wiley Periodicals, Inc.
-
experimental study of the connections of the telencephalon in the rainbow trout oncorhynchus mykiss ii dorsal area and preoptic region
The Journal of Comparative Neurology, 2004Co-Authors: Monica Folgueira, Ramon Anadon, Julian YanezAbstract:In this study and the accompanying article (Folgueira et al., 2004a), the fluorescent carbocyanine dye 1,1-dioctadecyl 3,3,3,3-tetramethylindocarbocyanine perchlorate (DiI) was used in fixed tissue to comprehensively analyze the connections of the different regions of the telencephalic lobes and the preoptic region of the rainbow trout. Here, we analyze the connections of the dorsal area (D; pallium) of the telencephalon, and the preoptic region, as well as the telencephalic connections of several structures in the diencephalon and brainstem of juvenile trout. The dorsal plus dorsolateral pallial zone of D (DdDl-d) receives afferents from contralateral DdDl-d, the ventral area of the telencephalon, preoptic Nucleus, suprachiasmatic Nucleus, medial thalamus, preglomerular complex, anterior and lateral tuberal nuclei, posterior tuberal Nucleus, posterior hypothalamic lobe, superior raphe Nucleus, and the rhombencephalic central gray and reticular formation, and projects to the central zone of D (Dc), medial thalamus, and some caudomedial hypothalamic regions. The medial zone of D (Dm) maintains reciprocal connections with the preglomerular complex and also receives afferents from the preoptic Nucleus, suprachiasmatic Nucleus, anterior tuberal Nucleus, preglomerular tertiary Gustatory Nucleus, posterior tubercle, superior raphe Nucleus, locus coeruleus, and the rhombencephalic central gray, and reticular formation. Dc receives fibers mainly from DdDl-d, preoptic Nucleus, preglomerular complex, and torus semicircularis and projects to several extratelencephalic centers, including the paracommissural Nucleus, optic tectum, torus semicircularis, thalamus, preglomerular complex, posterior tubercle nuclei, and inferior hypothalamic lobes. The posterior zone of D (Dp) is mainly connected with the olfactory bulbs, the ventral and supracommissural nuclei of the ventral area (subpallium), the preoptic Nucleus, and the preglomerular complex and projects to wide hypothalamic and posterior tubercular regions. The preoptic Nucleus projects to the olfactory bulb, to most regions of the telencephalic lobes, and to several diencephalic and brainstem structures. These results reveal complex and specialized connectional patterns in the rainbow trout dorsal telencephalon and preoptic region. Most of these connections have not been described previously in salmonids. These connections indicate that the salmonid telencephalon is involved in multisensorial processing and modulation of brain activity. J. Comp. Neurol. 480:204–233, 2004. © 2004 Wiley-Liss, Inc. Indexing terms: olfactory bulb; pallium; thalamus; torus semicircularis; cerebellum; teleost
-
experimental study of the connections of the Gustatory system in the rainbow trout oncorhynchus mykiss
The Journal of Comparative Neurology, 2003Co-Authors: Monica Folgueira, Ramon Anadon, Julian YanezAbstract:Salmonids are a group of teleosts with a nonspecialized Gustatory system. With the aim of describing the Gustatory connections in a member of this group, we carried out tract-tracing experiments using the lipophilic carbocyanine dye 1,1'-dioctadecyl 3,3,3',3'-tetramethylindocarbocyanine perchlorate (DiI) in fixed brains of the rainbow trout (Oncorhynchus mykiss). The neural tracer was applied to the primary viscerosensory column, secondary Gustatory visceral Nucleus (SGN), torus lateralis (TL), and tertiary Gustatory Nucleus (TGN), the dorsal part of the ventral area of the telencephalon (dorsal-Vv), and the medial area of the dorsal telencephalon (Dm). The primary viscerosensory column projects mainly to the SGN. DiI application to the SGN showed a bilateral and reciprocal connection with the TGN and a rostral portion of the Nucleus of the lateral hypothalamic recess. The application of DiI in the dorsal-Vv and Dm at levels rostral to the anterior commissure led to labeling of a restricted group of diencephalic neurons in the TGN and sending dendrites to the TL. DiI application to the TL/TGN anterogradely labeled fibers that coursed in the medial forebrain bundle innervating the precommissural portion of the dorsal-Vv and Dm. Caudally, this type of application led to labeling of fibers in the viscerosensory column and perikarya in the SGN. Tract-tracing results showed direct projections from the diencephalic and rhombencephalic Gustatory nuclei to the telencephalon. There was a direct and reciprocal connection between the SGN and the ventral telencephalon. The results showed that the Gustatory connections of the trout are similar to those of teleosts, with highly specialized Gustatory centers as in cyprinids and ictalurids, and to that observed in the percomorph tilapia, thus demonstrating a basic organization that is shared by most teleosts.
-
distribution of choline acetyltransferase chat immunoreactivity in the brain of the adult trout and tract tracing observations on the connections of the nuclei of the isthmus
The Journal of Comparative Neurology, 2000Co-Authors: Silvia Eva Perez, Ramon Anadon, Julian Yanez, Oscar Marin, Agustin Gonzalez, Isabel RodriguezmoldesAbstract:The distribution of cholinergic neurons and fibers was studied in the brain and rostral spinal cord of the brown trout and rainbow trout by using an antiserum against the enzyme choline acetyltransferase (ChAT). Cholinergic neurons were observed in the ventral telencephalon, preoptic region, habenula, thalamus, hypothalamus, magnocellular superficial pretectal Nucleus, optic tectum, isthmus, cranial nerve motor nuclei, and spinal cord. In addition, new cholinergic groups were detected in the vascular organ of the lamina terminalis, the parvocellular and magnocellular parts of the preoptic Nucleus, the anterior tuberal Nucleus, and a mesencephalic tegmental Nucleus. The presence of ChAT in the magnocellular neurosecretory system of trout suggests that acetylcholine is involved in control of hormone release by neurosecretory terminals. In order to characterize the several cholinergic nuclei observed in the isthmus of trout, their projections were studied by application of 1,1;-dioctadecyl-3,3,3;, 3;-tetramethylindocarbocyanine perchlorate (DiI) to selected structures of the brain. The secondary Gustatory Nucleus projected mainly to the lateral hypothalamic lobes, whereas the Nucleus isthmi projected to the optic tectum and parvocellular superficial pretectal Nucleus, as previously described in other teleost groups. In addition, other isthmic cholinergic nuclei of trout may be homologs of the mesopontine system of mammals. We conclude that the cholinergic systems of teleosts show many primitive features that have been preserved during evolution, together with characteristics exclusive to the group.
Joseph B. Travers - One of the best experts on this subject based on the ideXlab platform.
-
intrinsic membrane properties of pre oromotor neurons in the intermediate zone of the medullary reticular formation
Neuroscience, 2010Co-Authors: Sharmila Venugopal, Jack A Boulant, Zhixiong Chen, Joseph B. TraversAbstract:Abstract Neurons in the lower brainstem that control consummatory behavior are widely distributed in the reticular formation (RF) of the pons and medulla. The intrinsic membrane properties of neurons within this distributed system shape complex excitatory and inhibitory inputs from both orosensory and central structures implicated in homeostatic control to produce coordinated oromotor patterns. The current study explored the intrinsic membrane properties of neurons in the intermediate subdivision of the medullary reticular formation (IRt). Neurons in the IRt receive input from the overlying (Gustatory) Nucleus of the solitary tract and project to the oromotor nuclei. Recent behavioral pharmacology studies as well as computational modeling suggest that inhibition in the IRt plays an important role in the transition from a taste-initiated oromotor pattern of ingestion to one of rejection. The present study explored the impact of hyperpolarization on membrane properties. In response to depolarization, neurons responded with either a tonic discharge, an irregular/burst pattern or were spike-adaptive. A hyperpolarizing pre-pulse modulated the excitability of most (82%) IRt neurons to subsequent depolarization. Instances of both increased (30%) and decreased (52%) excitability were observed. Currents induced by the hyperpolarization included an outward 4-aminopyridine (4-AP) sensitive K + current that suppressed excitability and an inward cation current that increased excitability. These currents are also present in other subpopulations of RF neurons that influence the oromotor nuclei and we discuss how these currents could alter firing characteristics to impact pattern generation.
-
local circuit input to the medullary reticular formation from the rostral Nucleus of the solitary tract
American Journal of Physiology-regulatory Integrative and Comparative Physiology, 2008Co-Authors: Jason S Nasse, David Terman, Sharmila Venugopal, Gerlinda E Hermann, Richard C Rogers, Joseph B. TraversAbstract:The intermediate reticular formation (IRt) subjacent to the rostral (Gustatory) Nucleus of the solitary tract (rNST) receives projections from the rNST and appears essential to the expression of taste-elicited ingestion and rejection responses. We used whole cell patch-clamp recording and calcium imaging to characterize responses from an identified population of prehypoglossal neurons in the IRt to electrical stimulation of the rNST in a neonatal rat pup slice preparation. The calcium imaging studies indicated that IRt neurons could be activated by rNST stimulation and that many neurons were under tonic inhibition. Whole cell patch-clamp recording revealed mono- and polysynaptic projections from the rNST to identified prehypoglossal neurons. The projection was primarily excitatory and glutamatergic; however, there were some inhibitory GABAergic projections, and many neurons received excitatory and inhibitory inputs. There was also evidence of disinhibition. Overall, bath application of GABAA antagonists increased the amplitude of excitatory currents, and, in several neurons, stimulation of the rNST systematically decreased inhibitory currents. We have hypothesized that the transition from licks to gapes by natural stimuli, such as quinine monohydrochloride, could occur via such disinhibition. We present an updated dynamic model that summarizes the complex synaptic interface between the rNST and the IRt and demonstrates how inhibition could contribute to the transition from ingestion to rejection.
-
ascending and descending projections from the rostral Nucleus of the solitary tract originate from separate neuronal populations
Neuroscience, 1996Co-Authors: Christopher B Halsell, Susan P Travers, Joseph B. TraversAbstract:Abstract Anterograde studies have shown that neurons within the rostra] (Gustatory) Nucleus of the solitary tract project to the parabrachial Nucleus, as well as to sites within the medulla including the reticular formation and caudal Nucleus of the solitary tract. In order to determine the degree to which the same neurons contribute to both projections, injections of retrograde tracers were made simultaneously into both the parabrachial nuclei and medullary reticular formation of the rat. Only a small proportion of neurons were double labeled. Consistent with studies in hamster, labeled neurons projecting to the parabrachial nuclei in rat consisted of both stellate and elongate neurons, concentrated within the central subdivision of the rostral Nucleus of the solitary tract. Injections into the medullary reticular formation also labeled both stellate and elongate neurons but these were concentrated in the ventral subdivision of the Nucleus. The results of the present study demonstrate that different populations of neurons in the Nucleus of the solitary tract contribute to ascending and descending pathways. This suggests a possbile functional specialization within the Nucleus of the solitary tract for those neurons whose output eventually reaches the forebrain compared to those neurons with local connections.
Ramon Anadon - One of the best experts on this subject based on the ideXlab platform.
-
Gustatory and general visceral centers and their connections in the brain of adult zebrafish a carbocyanine dye tract tracing study
The Journal of Comparative Neurology, 2017Co-Authors: Julian Yanez, Monica Folgueira, Yara Souto, Laura Pineiro, Ramon AnadonAbstract:The central connections of the Gustatory/general visceral system of the adult zebrafish (Danio rerio) were examined by means of carbocyanine dye tracing. Main primary Gustatory centers (facial and vagal lobes) received sensory projections from the facial and vagal nerves, respectively. The vagal nerve also projects to the commissural Nucleus of Cajal, a general visceral sensory center. These primary centers mainly project on a prominent secondary Gustatory and general visceral Nucleus (SGN/V) located in the isthmic region. Secondary projections on the SGN/V were topographically organized, those of the facial lobe mainly ending medially to those of the vagal lobe, and those from the commissural Nucleus ventrolaterally. Descending facial lobe projections to the medial funicular Nucleus were also noted. Ascending fibers originating from the SGN/V mainly projected to the posterior thalamic Nucleus and the lateral hypothalamus (lateral torus, lateral recess Nucleus, hypothalamic inferior lobe diffuse Nucleus) and an intermediate cell- and fiber-rich region termed here the tertiary Gustatory Nucleus proper, but not to a Nucleus formerly considered as the zebrafish tertiary Gustatory Nucleus. The posterior thalamic Nucleus, tertiary Gustatory Nucleus proper, and Nucleus of the lateral recess gave rise to descending projections to the SGN/V and the vagal lobe. The connectivity between diencephalic Gustatory centers and the telencephalon was also investigated. The present results showed that the Gustatory connections of the adult zebrafish are rather similar to those reported in other cyprinids, excepting the tertiary Gustatory Nucleus. Similarities between the Gustatory systems of zebrafish and other fishes are also discussed. J. Comp. Neurol. 525:333-362, 2017. © 2016 Wiley Periodicals, Inc.
-
experimental study of the connections of the telencephalon in the rainbow trout oncorhynchus mykiss ii dorsal area and preoptic region
The Journal of Comparative Neurology, 2004Co-Authors: Monica Folgueira, Ramon Anadon, Julian YanezAbstract:In this study and the accompanying article (Folgueira et al., 2004a), the fluorescent carbocyanine dye 1,1-dioctadecyl 3,3,3,3-tetramethylindocarbocyanine perchlorate (DiI) was used in fixed tissue to comprehensively analyze the connections of the different regions of the telencephalic lobes and the preoptic region of the rainbow trout. Here, we analyze the connections of the dorsal area (D; pallium) of the telencephalon, and the preoptic region, as well as the telencephalic connections of several structures in the diencephalon and brainstem of juvenile trout. The dorsal plus dorsolateral pallial zone of D (DdDl-d) receives afferents from contralateral DdDl-d, the ventral area of the telencephalon, preoptic Nucleus, suprachiasmatic Nucleus, medial thalamus, preglomerular complex, anterior and lateral tuberal nuclei, posterior tuberal Nucleus, posterior hypothalamic lobe, superior raphe Nucleus, and the rhombencephalic central gray and reticular formation, and projects to the central zone of D (Dc), medial thalamus, and some caudomedial hypothalamic regions. The medial zone of D (Dm) maintains reciprocal connections with the preglomerular complex and also receives afferents from the preoptic Nucleus, suprachiasmatic Nucleus, anterior tuberal Nucleus, preglomerular tertiary Gustatory Nucleus, posterior tubercle, superior raphe Nucleus, locus coeruleus, and the rhombencephalic central gray, and reticular formation. Dc receives fibers mainly from DdDl-d, preoptic Nucleus, preglomerular complex, and torus semicircularis and projects to several extratelencephalic centers, including the paracommissural Nucleus, optic tectum, torus semicircularis, thalamus, preglomerular complex, posterior tubercle nuclei, and inferior hypothalamic lobes. The posterior zone of D (Dp) is mainly connected with the olfactory bulbs, the ventral and supracommissural nuclei of the ventral area (subpallium), the preoptic Nucleus, and the preglomerular complex and projects to wide hypothalamic and posterior tubercular regions. The preoptic Nucleus projects to the olfactory bulb, to most regions of the telencephalic lobes, and to several diencephalic and brainstem structures. These results reveal complex and specialized connectional patterns in the rainbow trout dorsal telencephalon and preoptic region. Most of these connections have not been described previously in salmonids. These connections indicate that the salmonid telencephalon is involved in multisensorial processing and modulation of brain activity. J. Comp. Neurol. 480:204–233, 2004. © 2004 Wiley-Liss, Inc. Indexing terms: olfactory bulb; pallium; thalamus; torus semicircularis; cerebellum; teleost
-
experimental study of the connections of the Gustatory system in the rainbow trout oncorhynchus mykiss
The Journal of Comparative Neurology, 2003Co-Authors: Monica Folgueira, Ramon Anadon, Julian YanezAbstract:Salmonids are a group of teleosts with a nonspecialized Gustatory system. With the aim of describing the Gustatory connections in a member of this group, we carried out tract-tracing experiments using the lipophilic carbocyanine dye 1,1'-dioctadecyl 3,3,3',3'-tetramethylindocarbocyanine perchlorate (DiI) in fixed brains of the rainbow trout (Oncorhynchus mykiss). The neural tracer was applied to the primary viscerosensory column, secondary Gustatory visceral Nucleus (SGN), torus lateralis (TL), and tertiary Gustatory Nucleus (TGN), the dorsal part of the ventral area of the telencephalon (dorsal-Vv), and the medial area of the dorsal telencephalon (Dm). The primary viscerosensory column projects mainly to the SGN. DiI application to the SGN showed a bilateral and reciprocal connection with the TGN and a rostral portion of the Nucleus of the lateral hypothalamic recess. The application of DiI in the dorsal-Vv and Dm at levels rostral to the anterior commissure led to labeling of a restricted group of diencephalic neurons in the TGN and sending dendrites to the TL. DiI application to the TL/TGN anterogradely labeled fibers that coursed in the medial forebrain bundle innervating the precommissural portion of the dorsal-Vv and Dm. Caudally, this type of application led to labeling of fibers in the viscerosensory column and perikarya in the SGN. Tract-tracing results showed direct projections from the diencephalic and rhombencephalic Gustatory nuclei to the telencephalon. There was a direct and reciprocal connection between the SGN and the ventral telencephalon. The results showed that the Gustatory connections of the trout are similar to those of teleosts, with highly specialized Gustatory centers as in cyprinids and ictalurids, and to that observed in the percomorph tilapia, thus demonstrating a basic organization that is shared by most teleosts.
-
distribution of choline acetyltransferase chat immunoreactivity in the brain of the adult trout and tract tracing observations on the connections of the nuclei of the isthmus
The Journal of Comparative Neurology, 2000Co-Authors: Silvia Eva Perez, Ramon Anadon, Julian Yanez, Oscar Marin, Agustin Gonzalez, Isabel RodriguezmoldesAbstract:The distribution of cholinergic neurons and fibers was studied in the brain and rostral spinal cord of the brown trout and rainbow trout by using an antiserum against the enzyme choline acetyltransferase (ChAT). Cholinergic neurons were observed in the ventral telencephalon, preoptic region, habenula, thalamus, hypothalamus, magnocellular superficial pretectal Nucleus, optic tectum, isthmus, cranial nerve motor nuclei, and spinal cord. In addition, new cholinergic groups were detected in the vascular organ of the lamina terminalis, the parvocellular and magnocellular parts of the preoptic Nucleus, the anterior tuberal Nucleus, and a mesencephalic tegmental Nucleus. The presence of ChAT in the magnocellular neurosecretory system of trout suggests that acetylcholine is involved in control of hormone release by neurosecretory terminals. In order to characterize the several cholinergic nuclei observed in the isthmus of trout, their projections were studied by application of 1,1;-dioctadecyl-3,3,3;, 3;-tetramethylindocarbocyanine perchlorate (DiI) to selected structures of the brain. The secondary Gustatory Nucleus projected mainly to the lateral hypothalamic lobes, whereas the Nucleus isthmi projected to the optic tectum and parvocellular superficial pretectal Nucleus, as previously described in other teleost groups. In addition, other isthmic cholinergic nuclei of trout may be homologs of the mesopontine system of mammals. We conclude that the cholinergic systems of teleosts show many primitive features that have been preserved during evolution, together with characteristics exclusive to the group.
Monica Folgueira - One of the best experts on this subject based on the ideXlab platform.
-
Gustatory and general visceral centers and their connections in the brain of adult zebrafish a carbocyanine dye tract tracing study
The Journal of Comparative Neurology, 2017Co-Authors: Julian Yanez, Monica Folgueira, Yara Souto, Laura Pineiro, Ramon AnadonAbstract:The central connections of the Gustatory/general visceral system of the adult zebrafish (Danio rerio) were examined by means of carbocyanine dye tracing. Main primary Gustatory centers (facial and vagal lobes) received sensory projections from the facial and vagal nerves, respectively. The vagal nerve also projects to the commissural Nucleus of Cajal, a general visceral sensory center. These primary centers mainly project on a prominent secondary Gustatory and general visceral Nucleus (SGN/V) located in the isthmic region. Secondary projections on the SGN/V were topographically organized, those of the facial lobe mainly ending medially to those of the vagal lobe, and those from the commissural Nucleus ventrolaterally. Descending facial lobe projections to the medial funicular Nucleus were also noted. Ascending fibers originating from the SGN/V mainly projected to the posterior thalamic Nucleus and the lateral hypothalamus (lateral torus, lateral recess Nucleus, hypothalamic inferior lobe diffuse Nucleus) and an intermediate cell- and fiber-rich region termed here the tertiary Gustatory Nucleus proper, but not to a Nucleus formerly considered as the zebrafish tertiary Gustatory Nucleus. The posterior thalamic Nucleus, tertiary Gustatory Nucleus proper, and Nucleus of the lateral recess gave rise to descending projections to the SGN/V and the vagal lobe. The connectivity between diencephalic Gustatory centers and the telencephalon was also investigated. The present results showed that the Gustatory connections of the adult zebrafish are rather similar to those reported in other cyprinids, excepting the tertiary Gustatory Nucleus. Similarities between the Gustatory systems of zebrafish and other fishes are also discussed. J. Comp. Neurol. 525:333-362, 2017. © 2016 Wiley Periodicals, Inc.
-
experimental study of the connections of the telencephalon in the rainbow trout oncorhynchus mykiss ii dorsal area and preoptic region
The Journal of Comparative Neurology, 2004Co-Authors: Monica Folgueira, Ramon Anadon, Julian YanezAbstract:In this study and the accompanying article (Folgueira et al., 2004a), the fluorescent carbocyanine dye 1,1-dioctadecyl 3,3,3,3-tetramethylindocarbocyanine perchlorate (DiI) was used in fixed tissue to comprehensively analyze the connections of the different regions of the telencephalic lobes and the preoptic region of the rainbow trout. Here, we analyze the connections of the dorsal area (D; pallium) of the telencephalon, and the preoptic region, as well as the telencephalic connections of several structures in the diencephalon and brainstem of juvenile trout. The dorsal plus dorsolateral pallial zone of D (DdDl-d) receives afferents from contralateral DdDl-d, the ventral area of the telencephalon, preoptic Nucleus, suprachiasmatic Nucleus, medial thalamus, preglomerular complex, anterior and lateral tuberal nuclei, posterior tuberal Nucleus, posterior hypothalamic lobe, superior raphe Nucleus, and the rhombencephalic central gray and reticular formation, and projects to the central zone of D (Dc), medial thalamus, and some caudomedial hypothalamic regions. The medial zone of D (Dm) maintains reciprocal connections with the preglomerular complex and also receives afferents from the preoptic Nucleus, suprachiasmatic Nucleus, anterior tuberal Nucleus, preglomerular tertiary Gustatory Nucleus, posterior tubercle, superior raphe Nucleus, locus coeruleus, and the rhombencephalic central gray, and reticular formation. Dc receives fibers mainly from DdDl-d, preoptic Nucleus, preglomerular complex, and torus semicircularis and projects to several extratelencephalic centers, including the paracommissural Nucleus, optic tectum, torus semicircularis, thalamus, preglomerular complex, posterior tubercle nuclei, and inferior hypothalamic lobes. The posterior zone of D (Dp) is mainly connected with the olfactory bulbs, the ventral and supracommissural nuclei of the ventral area (subpallium), the preoptic Nucleus, and the preglomerular complex and projects to wide hypothalamic and posterior tubercular regions. The preoptic Nucleus projects to the olfactory bulb, to most regions of the telencephalic lobes, and to several diencephalic and brainstem structures. These results reveal complex and specialized connectional patterns in the rainbow trout dorsal telencephalon and preoptic region. Most of these connections have not been described previously in salmonids. These connections indicate that the salmonid telencephalon is involved in multisensorial processing and modulation of brain activity. J. Comp. Neurol. 480:204–233, 2004. © 2004 Wiley-Liss, Inc. Indexing terms: olfactory bulb; pallium; thalamus; torus semicircularis; cerebellum; teleost
-
experimental study of the connections of the Gustatory system in the rainbow trout oncorhynchus mykiss
The Journal of Comparative Neurology, 2003Co-Authors: Monica Folgueira, Ramon Anadon, Julian YanezAbstract:Salmonids are a group of teleosts with a nonspecialized Gustatory system. With the aim of describing the Gustatory connections in a member of this group, we carried out tract-tracing experiments using the lipophilic carbocyanine dye 1,1'-dioctadecyl 3,3,3',3'-tetramethylindocarbocyanine perchlorate (DiI) in fixed brains of the rainbow trout (Oncorhynchus mykiss). The neural tracer was applied to the primary viscerosensory column, secondary Gustatory visceral Nucleus (SGN), torus lateralis (TL), and tertiary Gustatory Nucleus (TGN), the dorsal part of the ventral area of the telencephalon (dorsal-Vv), and the medial area of the dorsal telencephalon (Dm). The primary viscerosensory column projects mainly to the SGN. DiI application to the SGN showed a bilateral and reciprocal connection with the TGN and a rostral portion of the Nucleus of the lateral hypothalamic recess. The application of DiI in the dorsal-Vv and Dm at levels rostral to the anterior commissure led to labeling of a restricted group of diencephalic neurons in the TGN and sending dendrites to the TL. DiI application to the TL/TGN anterogradely labeled fibers that coursed in the medial forebrain bundle innervating the precommissural portion of the dorsal-Vv and Dm. Caudally, this type of application led to labeling of fibers in the viscerosensory column and perikarya in the SGN. Tract-tracing results showed direct projections from the diencephalic and rhombencephalic Gustatory nuclei to the telencephalon. There was a direct and reciprocal connection between the SGN and the ventral telencephalon. The results showed that the Gustatory connections of the trout are similar to those of teleosts, with highly specialized Gustatory centers as in cyprinids and ictalurids, and to that observed in the percomorph tilapia, thus demonstrating a basic organization that is shared by most teleosts.