The Experts below are selected from a list of 42 Experts worldwide ranked by ideXlab platform
Bengt Magnusson - One of the best experts on this subject based on the ideXlab platform.
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gas chromatography mass spectrometry determination of the pentafluorobenzoyl derivative of methylhydrazine in false morel gyromitra esculenta as a monitor for the content of the toxin Gyromitrin
Journal of Chromatography A, 2006Co-Authors: Mehrdad Arshadi, Calle Nilsson, Bengt MagnussonAbstract:The main toxic compound found in false morel (Gyromitra esculenta) is acetaldehyde-N-methyl-N-formylhydrazone (Gyromitrin). This paper describes a method of determining the total hydrazones content based on acid hydrolysis of Gyromitrin and other related hydrazones in air-dried false morel followed by derivatisation of methylhydrazine with pentafluorobenzoyl chloride. The derivative, tris-pentafluorobenzoyl methylhydrazine (tris-PFB-MH) is analyzed by gas chromatography–mass spectrometry. The overall precision of the method is better than 10% (relative standard deviation) for 0.5 ng/μl methylhydrazine in solution. The minimum detectable concentration of methylhydrazine (tris-PFB-MH) by this method is estimated to be approximately 12 pg/μl, which is equal to 0.3 μg/g dry matter (DM) of false morel.
Mehrdad Arshadi - One of the best experts on this subject based on the ideXlab platform.
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gas chromatography mass spectrometry determination of the pentafluorobenzoyl derivative of methylhydrazine in false morel gyromitra esculenta as a monitor for the content of the toxin Gyromitrin
Journal of Chromatography A, 2006Co-Authors: Mehrdad Arshadi, Calle Nilsson, Bengt MagnussonAbstract:The main toxic compound found in false morel (Gyromitra esculenta) is acetaldehyde-N-methyl-N-formylhydrazone (Gyromitrin). This paper describes a method of determining the total hydrazones content based on acid hydrolysis of Gyromitrin and other related hydrazones in air-dried false morel followed by derivatisation of methylhydrazine with pentafluorobenzoyl chloride. The derivative, tris-pentafluorobenzoyl methylhydrazine (tris-PFB-MH) is analyzed by gas chromatography–mass spectrometry. The overall precision of the method is better than 10% (relative standard deviation) for 0.5 ng/μl methylhydrazine in solution. The minimum detectable concentration of methylhydrazine (tris-PFB-MH) by this method is estimated to be approximately 12 pg/μl, which is equal to 0.3 μg/g dry matter (DM) of false morel.
Karlerik Hellenas - One of the best experts on this subject based on the ideXlab platform.
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methylation of rat and mouse dna by the mushroom poison Gyromitrin and its metabolite monomethylhydrazine
Cancer Letters, 1992Co-Authors: Kerstin Bergman, Karlerik HellenasAbstract:Abstract Consumption of false morel (Gyromitra esculenta Fr.) has been associated not only with acute poisoning, but also with a carcinogenic risk. The hydrolysis of acetaldehyde-N-methyl-N-formylhydrazone (Gyromitrin, the main toxic component of false morel) results in the formation of the methylating agents N-methyl-N-formylhydrazine (MFH) and N-methylhydrazine (MMH) (by further hydrolysis of MFH). This study reports traces of N-7-methylguanine (N7MeGu) in liver DNA from mice and a rat treated with Gyromitrin. After repeated administration of MMH, N7MeGu was identified in rat liver DNA. In mice exposed to MMH according to a dosing scheme identical to that reported to induce tumours in this species, O 6 -methylguanine was present in liver and kidney DNA. The results indicate that a relatively low carcinogenic risk is associated with false morel consumption. The risk may be greater in individuals with a decreased detoxification rate (acetylation) of MFH, in whom larger amounts of MMH are formed from Gyromitrin.
Calle Nilsson - One of the best experts on this subject based on the ideXlab platform.
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gas chromatography mass spectrometry determination of the pentafluorobenzoyl derivative of methylhydrazine in false morel gyromitra esculenta as a monitor for the content of the toxin Gyromitrin
Journal of Chromatography A, 2006Co-Authors: Mehrdad Arshadi, Calle Nilsson, Bengt MagnussonAbstract:The main toxic compound found in false morel (Gyromitra esculenta) is acetaldehyde-N-methyl-N-formylhydrazone (Gyromitrin). This paper describes a method of determining the total hydrazones content based on acid hydrolysis of Gyromitrin and other related hydrazones in air-dried false morel followed by derivatisation of methylhydrazine with pentafluorobenzoyl chloride. The derivative, tris-pentafluorobenzoyl methylhydrazine (tris-PFB-MH) is analyzed by gas chromatography–mass spectrometry. The overall precision of the method is better than 10% (relative standard deviation) for 0.5 ng/μl methylhydrazine in solution. The minimum detectable concentration of methylhydrazine (tris-PFB-MH) by this method is estimated to be approximately 12 pg/μl, which is equal to 0.3 μg/g dry matter (DM) of false morel.
Kerstin Bergman - One of the best experts on this subject based on the ideXlab platform.
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methylation of rat and mouse dna by the mushroom poison Gyromitrin and its metabolite monomethylhydrazine
Cancer Letters, 1992Co-Authors: Kerstin Bergman, Karlerik HellenasAbstract:Abstract Consumption of false morel (Gyromitra esculenta Fr.) has been associated not only with acute poisoning, but also with a carcinogenic risk. The hydrolysis of acetaldehyde-N-methyl-N-formylhydrazone (Gyromitrin, the main toxic component of false morel) results in the formation of the methylating agents N-methyl-N-formylhydrazine (MFH) and N-methylhydrazine (MMH) (by further hydrolysis of MFH). This study reports traces of N-7-methylguanine (N7MeGu) in liver DNA from mice and a rat treated with Gyromitrin. After repeated administration of MMH, N7MeGu was identified in rat liver DNA. In mice exposed to MMH according to a dosing scheme identical to that reported to induce tumours in this species, O 6 -methylguanine was present in liver and kidney DNA. The results indicate that a relatively low carcinogenic risk is associated with false morel consumption. The risk may be greater in individuals with a decreased detoxification rate (acetylation) of MFH, in whom larger amounts of MMH are formed from Gyromitrin.