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H Kucuk - One of the best experts on this subject based on the ideXlab platform.
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cryopreservation trial with semen of purebred arabian and Haflinger stallions in the turkish national stud in karacabey
Deutsche Tierarztliche Wochenschrift, 1993Co-Authors: N Tekin, N Yurdaydin, E Klug, Ali Daskin, O Keskin, H KucukAbstract:Within a German-Turkish university partnership deep freezing preservation of stallion semen was performed as a part project of the cooperation contract. In this study a modification of the introduced Makrotub method was used for semen freezing. The investigated characteristics of fresh semen of the Arab stallions were in the normal range cited in the international literature. However, the semen data obtained from the Haflinger stallions were markedly and partially significantly in lower range than measured for the Arab stallions. This may reflect an incomplete adaptation process of the imported Haflinger horse population. Local circumstances did not allow Al trials.
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karacabey tarim isletmesinde yetistirilen arap Haflinger ve arap x Haflinger fi melezi atlarin bazi verim ozellikleri uzerinde arastirmalar 1 dol verimi ve yasama gucu
Lalahan Hayvancılık Araştırma Enstitüsü Dergisi, 1992Co-Authors: H Kucuk, Ahmet AltinelAbstract:Bu calisma Karacabey Tarim Isletmesinde yetistirilen Arap, Haflinger ve Arap x Haflinger F1 atlarin verim duzeylerinin saptanmasi amaciyla yurutulen bir arastirmanin birinci bolumu ile ilgilidir. Bu bolumde kisraklarda dol verimi ile taylarda yasama gucu uzerinde durulmustur. Arastirmada dolverimi ozellikleri 1983-1987 yillari arasindaki verilere dayanilarak 309 adet arap, 338 adet Haflinger ve 61 adet Arap x Haflinger F1 kisrak uzerinde, yasama gucu ise 1987-1988 yillarinda canli dogan 111 adet Arap, 73 adet Haflinger ve 31 adet arap x Haflinger Fi tay uzerinde incelenmistir. Aygir alti kisrak sayisina gore gebelik ve dogum oranlari, Arap kisraklarda % 81.9 ve % 80.0, Haflinger kisraklarda % 74.9 ve % 67.8, Arap x Haflinger F1 kisraklarda ise % 74.2 ve % 74.2 duzeyinde bulunmustur. Taylarda yasama gucu, dogumdan itibaren 3., 6. ve 24. aya kadar Araplarda sirasiyla % 94.6, % 92.8 ve % 91.0, Haflingerlerde % 97.3, % 95.9 ve % 95.9, arap x Haflinger F1’lerde ise yine ayni siraya gore % 100.0, % 94.4 ve % 94.4 duzeyinde saptanmistir.
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karacabey tarim isletmesinde yetistirilen arap Haflinger ve arap x Haflinger fi melezi atlarin bazi verim ozellikleri uzerinde arastirmalar ii buyume ve canli ağirlik
Lalahan Hayvancılık Araştırma Enstitüsü Dergisi, 1992Co-Authors: Ahmet Altinel, H KucukAbstract:Bu calisma Karacabey Tarim Isletmesinde yetistirilen Arap, Haflinger ve Arap x Haflinger F1 atlarin verim duzeylerinin saptanmasi amaciyla yurutulen bir arastirmanin ikinci bolumu ile ilgilidir. Bu bolumde atlarda buyume ve kisraklarda canli agirlik ozellikleri uzerinde durulmustur. Buyume ve vucut olculeri degerleri toplam 322 tayin 1987-1988 yillarina ait verileri uzerinde incelenmistir. Canli agirliklar; Arap erkek taylarda; dogum, 12. ve 24. aylarda sirasiyla 46.35, 276.74 ve 377.27 kg, disi taylarda ise dogum, 12., 24. ve 36. aylarda sirasiyla 45.65, 264.67, 356.21 ve 402.00 kg bulunmustur. Haflinger erkek taylar dogum, 12. ve 24. ayda sirasiyla 39.05, 242.80 ve 381.71 kg, disi taylarda ise dogum 12., 24. ve 36. aylarda 38.50, 249.79, 345.79 ve 358.85 kg duzeyinde olmuslardir. Arap x Haflinger F1 erkek taylar, dogum ve 12. ayda 40.07 ve 248.00 kg, disi taylar ise dogum 12., 24. ve 36. aylarda sirasiyla 40.50, 257.88, 350.38 ve 371.25 kg agirlikta saptanmislardir. Buyume ve vucut olculeri uzerindeki etkiler olarak ana yasi, cinsiyet ve genotip uzerinde durulmustur. Ana yasinin etkisi 3. ay agirligi ve on incik cevresi uzerinde onemli bulunmustur. Cinsiyetin etkisi; 3. ay canli agirlik, 18. ay cidago yuksekligi, 6. ay on incik cevresi uzerinde onemli olmustur. Genotipin etkisi ise gogus cevresi ve cidago yuksekligi uzerinde, hemen butun buyume donemlerinde onemli bulunmustur.
Jean-luc Gaillard - One of the best experts on this subject based on the ideXlab platform.
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Equine alpha S1-casein: Characterization of alternative splicing isoforms and determination of phosphorylation levels
Journal of Dairy Science, 2009Co-Authors: Aurélie Matéos, Jean-michel Girardet, Daniel Mollé, Laurent Miclo, Annie Mourot, Jean-luc GaillardAbstract:αS1-Casein was isolated from Haflinger mare’s milk by hydrophobic interaction chromatography and displayed great micro-heterogeneity by 2-dimensional electrophoresis, probably because of a variable degree of phosphorylation and alternative splicing events. The aim of the present work was to investigate the complexity of the mare’s αS1-casein. The different isoforms present in milk were submitted to a double treatment of dephosphorylation, first by using alkaline phosphatase and then acid phosphatase to achieve complete dephosphorylation. The apoforms were then analyzed by electrospray ionization mass spectrometry. The results revealed the existence of a full-length protein and 7 variants resulting from posttranscriptional modifications; that is, exon skipping involving exon 7, exon 14, or both and use of a cryptic splice site encoding a glutamine residue. The determination of the different phosphorylation degrees of the native isoforms of αS1- casein was finally achieved by electrospray ionization mass spectrometry analysis after fractionation of the isoforms by ion-exchange chromatography. Thus, 36 different variants of equine αS1-casein were identified with several phosphate groups ranging from 2 to 6 or 8 depending on whether exon 7 was skipped. Key words: alternative splicing , cryptic splice site , equine αS1-casein , phosphorylation
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the primary structure of a low mr multiphosphorylated variant of β casein in equine milk
Proteomics, 2007Co-Authors: Antonio Silvio Do Egito, Jean-michel Girardet, Daniel Mollé, Laurent Miclo, Patrice Martin, Jean-luc GaillardAbstract:Highly phosphorylated casein with a low molecular mass was isolated from Haflinger mare's milk by RP-HPLC. It accounts for 4.0% of the casein content. Its mass was determined by LC-ESI-MS before and after treatment by alkaline phosphatase. The molecular mass found for the apo-form (10,591 +/- 2 Da) is in agreement with its primary structure, which was established by ESI-MS/MS from tryptic peptides. It appeared that this short protein (94 amino acid residues) is an internally truncated form of the full-length equine beta-casein (226 residues). This low-Mr variant of equine beta-casein displays a large deletion (residues 50-181), due to a cryptic splice site usage occurring within exon 7 during the course of primary transcripts processing. The phosphorylation pattern of this equine beta-casein variant was investigated by LC-ESI-MS and 2-DE. Seven phosphorylation forms were identified with one to seven phosphate groups with pIs ranging between 4.67 and 4.01. The major isoforms carry five and six phosphate groups.
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Determination of the phosphorylation level and deamidation susceptibility of equine beta-casein
Proteomics, 2006Co-Authors: Jean-michel Girardet, Daniel Mollé, Laurent Miclo, Sabrina Florent, Jean-luc GaillardAbstract:beta-Casein was isolated from Haflinger mare’s milk by RP-HPLC, and displayed microheterogeneity by urea-electrophoresis and 2-DE probably due to a variable degree of phosphorylation. To investigate the degree of phosphorylation, the primary structure of equine b-casein was determined by tryptic hydrolysis and MS of peptides released and by MS of the protein treated by alkaline phosphatase. The molecular mass found for the apo-form of Haflinger mare’s b-casein (25 514 6 3 Da) was close to the theoretical mass of the reported sequence (GenBank AAG43954) modified by insertion of a region (residues 27–34) encoded by an exon sometimes out-spliced (25 511.40 Da). Hence, the beta-casein isolated from Haflinger mare’s milk corresponded to a variant of 226 amino acid residues. The latter was composed by highly multi-phosphorylated isoforms with three to seven phosphate groups, and pIs, determined by 2-DE, ranging from 4.74 to 5.30.Moreover, the equine beta-casein was able to deamidate spontaneously, at the level of Asn in the potential deamidation motif 135Asn-Gly136. Approximately 80% of the protein was deamidated after 96 h of incubation under physiological conditions.
Laurent Miclo - One of the best experts on this subject based on the ideXlab platform.
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Equine alpha S1-casein: Characterization of alternative splicing isoforms and determination of phosphorylation levels
Journal of Dairy Science, 2009Co-Authors: Aurélie Matéos, Jean-michel Girardet, Daniel Mollé, Laurent Miclo, Annie Mourot, Jean-luc GaillardAbstract:αS1-Casein was isolated from Haflinger mare’s milk by hydrophobic interaction chromatography and displayed great micro-heterogeneity by 2-dimensional electrophoresis, probably because of a variable degree of phosphorylation and alternative splicing events. The aim of the present work was to investigate the complexity of the mare’s αS1-casein. The different isoforms present in milk were submitted to a double treatment of dephosphorylation, first by using alkaline phosphatase and then acid phosphatase to achieve complete dephosphorylation. The apoforms were then analyzed by electrospray ionization mass spectrometry. The results revealed the existence of a full-length protein and 7 variants resulting from posttranscriptional modifications; that is, exon skipping involving exon 7, exon 14, or both and use of a cryptic splice site encoding a glutamine residue. The determination of the different phosphorylation degrees of the native isoforms of αS1- casein was finally achieved by electrospray ionization mass spectrometry analysis after fractionation of the isoforms by ion-exchange chromatography. Thus, 36 different variants of equine αS1-casein were identified with several phosphate groups ranging from 2 to 6 or 8 depending on whether exon 7 was skipped. Key words: alternative splicing , cryptic splice site , equine αS1-casein , phosphorylation
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the primary structure of a low mr multiphosphorylated variant of β casein in equine milk
Proteomics, 2007Co-Authors: Antonio Silvio Do Egito, Jean-michel Girardet, Daniel Mollé, Laurent Miclo, Patrice Martin, Jean-luc GaillardAbstract:Highly phosphorylated casein with a low molecular mass was isolated from Haflinger mare's milk by RP-HPLC. It accounts for 4.0% of the casein content. Its mass was determined by LC-ESI-MS before and after treatment by alkaline phosphatase. The molecular mass found for the apo-form (10,591 +/- 2 Da) is in agreement with its primary structure, which was established by ESI-MS/MS from tryptic peptides. It appeared that this short protein (94 amino acid residues) is an internally truncated form of the full-length equine beta-casein (226 residues). This low-Mr variant of equine beta-casein displays a large deletion (residues 50-181), due to a cryptic splice site usage occurring within exon 7 during the course of primary transcripts processing. The phosphorylation pattern of this equine beta-casein variant was investigated by LC-ESI-MS and 2-DE. Seven phosphorylation forms were identified with one to seven phosphate groups with pIs ranging between 4.67 and 4.01. The major isoforms carry five and six phosphate groups.
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Determination of the phosphorylation level and deamidation susceptibility of equine beta-casein
Proteomics, 2006Co-Authors: Jean-michel Girardet, Daniel Mollé, Laurent Miclo, Sabrina Florent, Jean-luc GaillardAbstract:beta-Casein was isolated from Haflinger mare’s milk by RP-HPLC, and displayed microheterogeneity by urea-electrophoresis and 2-DE probably due to a variable degree of phosphorylation. To investigate the degree of phosphorylation, the primary structure of equine b-casein was determined by tryptic hydrolysis and MS of peptides released and by MS of the protein treated by alkaline phosphatase. The molecular mass found for the apo-form of Haflinger mare’s b-casein (25 514 6 3 Da) was close to the theoretical mass of the reported sequence (GenBank AAG43954) modified by insertion of a region (residues 27–34) encoded by an exon sometimes out-spliced (25 511.40 Da). Hence, the beta-casein isolated from Haflinger mare’s milk corresponded to a variant of 226 amino acid residues. The latter was composed by highly multi-phosphorylated isoforms with three to seven phosphate groups, and pIs, determined by 2-DE, ranging from 4.74 to 5.30.Moreover, the equine beta-casein was able to deamidate spontaneously, at the level of Asn in the potential deamidation motif 135Asn-Gly136. Approximately 80% of the protein was deamidated after 96 h of incubation under physiological conditions.
Ahmet Altinel - One of the best experts on this subject based on the ideXlab platform.
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karacabey tarim isletmesinde yetistirilen arap Haflinger ve arap x Haflinger fi melezi atlarin bazi verim ozellikleri uzerinde arastirmalar 1 dol verimi ve yasama gucu
Lalahan Hayvancılık Araştırma Enstitüsü Dergisi, 1992Co-Authors: H Kucuk, Ahmet AltinelAbstract:Bu calisma Karacabey Tarim Isletmesinde yetistirilen Arap, Haflinger ve Arap x Haflinger F1 atlarin verim duzeylerinin saptanmasi amaciyla yurutulen bir arastirmanin birinci bolumu ile ilgilidir. Bu bolumde kisraklarda dol verimi ile taylarda yasama gucu uzerinde durulmustur. Arastirmada dolverimi ozellikleri 1983-1987 yillari arasindaki verilere dayanilarak 309 adet arap, 338 adet Haflinger ve 61 adet Arap x Haflinger F1 kisrak uzerinde, yasama gucu ise 1987-1988 yillarinda canli dogan 111 adet Arap, 73 adet Haflinger ve 31 adet arap x Haflinger Fi tay uzerinde incelenmistir. Aygir alti kisrak sayisina gore gebelik ve dogum oranlari, Arap kisraklarda % 81.9 ve % 80.0, Haflinger kisraklarda % 74.9 ve % 67.8, Arap x Haflinger F1 kisraklarda ise % 74.2 ve % 74.2 duzeyinde bulunmustur. Taylarda yasama gucu, dogumdan itibaren 3., 6. ve 24. aya kadar Araplarda sirasiyla % 94.6, % 92.8 ve % 91.0, Haflingerlerde % 97.3, % 95.9 ve % 95.9, arap x Haflinger F1’lerde ise yine ayni siraya gore % 100.0, % 94.4 ve % 94.4 duzeyinde saptanmistir.
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karacabey tarim isletmesinde yetistirilen arap Haflinger ve arap x Haflinger fi melezi atlarin bazi verim ozellikleri uzerinde arastirmalar ii buyume ve canli ağirlik
Lalahan Hayvancılık Araştırma Enstitüsü Dergisi, 1992Co-Authors: Ahmet Altinel, H KucukAbstract:Bu calisma Karacabey Tarim Isletmesinde yetistirilen Arap, Haflinger ve Arap x Haflinger F1 atlarin verim duzeylerinin saptanmasi amaciyla yurutulen bir arastirmanin ikinci bolumu ile ilgilidir. Bu bolumde atlarda buyume ve kisraklarda canli agirlik ozellikleri uzerinde durulmustur. Buyume ve vucut olculeri degerleri toplam 322 tayin 1987-1988 yillarina ait verileri uzerinde incelenmistir. Canli agirliklar; Arap erkek taylarda; dogum, 12. ve 24. aylarda sirasiyla 46.35, 276.74 ve 377.27 kg, disi taylarda ise dogum, 12., 24. ve 36. aylarda sirasiyla 45.65, 264.67, 356.21 ve 402.00 kg bulunmustur. Haflinger erkek taylar dogum, 12. ve 24. ayda sirasiyla 39.05, 242.80 ve 381.71 kg, disi taylarda ise dogum 12., 24. ve 36. aylarda 38.50, 249.79, 345.79 ve 358.85 kg duzeyinde olmuslardir. Arap x Haflinger F1 erkek taylar, dogum ve 12. ayda 40.07 ve 248.00 kg, disi taylar ise dogum 12., 24. ve 36. aylarda sirasiyla 40.50, 257.88, 350.38 ve 371.25 kg agirlikta saptanmislardir. Buyume ve vucut olculeri uzerindeki etkiler olarak ana yasi, cinsiyet ve genotip uzerinde durulmustur. Ana yasinin etkisi 3. ay agirligi ve on incik cevresi uzerinde onemli bulunmustur. Cinsiyetin etkisi; 3. ay canli agirlik, 18. ay cidago yuksekligi, 6. ay on incik cevresi uzerinde onemli olmustur. Genotipin etkisi ise gogus cevresi ve cidago yuksekligi uzerinde, hemen butun buyume donemlerinde onemli bulunmustur.
V. Russo - One of the best experts on this subject based on the ideXlab platform.
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SNPs within the beta myosin heavy chain (MYH7) and the pyruvate kinase muscle (PKM2) genes in horse
Taylor & Francis Group, 2010Co-Authors: V. Russo, R. Davoli, E. Scotti, L. Fontanesi, Stefania Dall'olioAbstract:Two highly expressed skeletal muscle genes (the MYH7 gene encoding the myosin heavy chain slow/β-cardiac isoform and the PKM2 gene encoding the pyruvate kinase muscle isoforms) were investigated with the objective to identify DNA markers in horses. A panel of DNA samples from different horse breeds was analysed using a PCR-single strand conformation polymorphism (SSCP) approach. Four and two alleles were identified for the MYH7 and PKM2 loci, respectively. Mendelian inheritance of alleles of the two investigated genes was confirmed analysing horse families. Sequencing of PCR products obtained from the MYH7 and PKM2 genes made it possible to characterise two SSCP alleles for each gene. The polymorphisms found in the MYH7 and PKM2 genes were further studied in 61 and 68 horses of three (Italian Heavy Draught Horse, Italian Saddler and Murgese) and five (Franches-Montagnes, Haflinger, Italian Heavy Draught Horse, Murgese and Standardbred) breeds, respectively. Allele frequencies of the two loci varied among the considered breeds. The SNPs discovery in MYH7 and PKM2 genes makes it possible to locate new molecular markers to ECA1. The identified markers could be used in association analysis with performance traits in horses
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SNPs within the beta myosin heavy chain (MYH7) and the pyruvate kinase muscle (PKM2) genes in horse
2007Co-Authors: S. Dall'olio, R. Davoli, E. Scotti, L. Fontanesi, V. RussoAbstract:Two highly expressed skeletal muscle genes (the MYH7 gene encoding the myosin heavy chain slow/b-cardiac isoform and the PKM2 gene encoding the pyruvate kinase muscle isoforms) were investigated with the objective to identify DNA markers in horses. A panel of DNA samples from different horse breeds was analysed using a PCR-single strand conformation polymorphism (SSCP) approach. Four and two alleles were identified for the MYH7 and PKM2 loci, respectively. Mendelian inheritance of alleles of the two investigated genes was confirmed analysing horse families. Sequencing of PCR products obtained from the MYH7 and PKM2 genes allowed to characterize two SSCP alleles for each gene. The polymorphisms found in the MYH7 and PKM2 genes were further studied in 61 and 68 horses of three (Italian Heavy Draught Horse, Italian Saddler and Murgese) and five (Franches-Montagnes, Haflinger, Italian Heavy Draught Horse, Murgese and Standardbred) breeds, respectively. Allele frequencies of the two loci varied among the considered breeds. The SNPs discovery in MYH7 and PKM2 genes allows to locate new molecular markers to ECA1. The identified markers could be used in association analysis with performance traits in horses
