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Elzbieta Romanowska - One of the best experts on this subject based on the ideXlab platform.
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Structure of the lipopolysaccharide core region of Hafnia alvei strains 1185 and 1204.
Carbohydrate Research, 2000Co-Authors: Joanna Kübler, Ewa Katzenellenbogen, Andrzej Gamian, Maria Bogulska, Andrzej Ejchart, Elzbieta RomanowskaAbstract:Abstract Sugar and methylation analyses using gas chromatography/mass spectrometry and NMR spectroscopy proved that the core oligosaccharides of Hafnia alvei strains 1185 and 1204 have the following formula: Download full-size image where Kdo=3-deoxy-oct-2-ulosonic acid and PPEtN=diphosphorylethanolamine. The structure shown above is a slight modification of the typical core region of H. alvei lipopolysaccharides. The difference refers to one sugar only: terminal galactose is present in the core of strains of 1185 and 1204, while terminal glucose in the typical core.
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Non-typical lipopolysaccharide core regions of some Hafnia alvei strains: structural and serological studies.
FEMS immunology and medical microbiology, 1999Co-Authors: Elzbieta Romanowska, Ewa Katzenellenbogen, Tomasz Niedziela, Wojciech Jachymek, Maria Bogulska, Czesław ŁugowskiAbstract:The lipopolysaccharides of Hafnia alvei strains 23, 1222 and 39 were found to have non-typical core region. On the basis of sugar and methylation analyses, 1H-nuclear magnetic resonance spectra and matrix-assisted laser-desorption ionization-time of flight mass spectrometry, it was concluded that the core oligosaccharide of strains 23 and 1222 has the same structure as Escherichia coli R4 core region, and the core oligosaccharide of strain 39 has the structure of Salmonella Ra core. Using the serological methods (passive hemagglutination, enzyme-linked immunosorbent assay and immunoblotting) and the anti-conjugate sera directed against E. coli R4 and Salmonella Ra core oligosaccharides we have confirmed the structural results presented above.
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Reinvestigation of the O-specific polysaccharides of Hafnia alvei lipopolysaccharides isolated from strains ATCC 13337 and 1187
Carbohydrate Research, 1998Co-Authors: Andrzej Gamian, Ewa Katzenellenbogen, Elzbieta Romanowska, H Grosskurth, Janusz DabrowskiAbstract:Abstract The structure of the O-specific polysaccharides of the lipopolysaccharides produced by Hafnia alvei strains ATCC 13337 and 1187 was reinvestigated. The position of phosphate group in the repeating units of the polysaccharides was established with the aid of 1 H detected, 31 P edited NMR spectra. According to the results obtained, the polysaccharides are teichoic acid-like polymers with the repeating units of the following structure: Download full-size image where Acyl= D -3-hydroxylbutyryl, and 3-O-acetylation was approximately 30%.
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Reinvestigation of the O-specific polysaccharides of Hafnia alvei lipopolysaccharides isolated from strains ATCC 13337 and 1187.
Carbohydrate research, 1998Co-Authors: Andrzej Gamian, Ewa Katzenellenbogen, Elzbieta Romanowska, H Grosskurth, Janusz DabrowskiAbstract:The structure of the O-specific polysaccharides of the lipopolysaccharides produced by Hafnia alvei strains ATCC 13337 and 1187 was reinvestigated. The position of phosphate group in the repeating units of the polysaccharides was established with the aid of 1H detected, 31P edited NMR spectra. According to the results obtained, the polysaccharides are teichoic acid-like polymers with the repeating units of the following structure: [formula: see text] where Acyl = D-3-hydroxylbutyryl, and 3-O-acetylation was approximately 30%.
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Structure of the O-specific polysaccharide, containing a glycerol phosphate substituent, of Hafnia alvei strain 1220 lipopolysaccharide
Carbohydrate research, 1996Co-Authors: Ursula Dabrowski, Ewa Katzenellenbogen, Maria Bogulska, Janusz Dabrowski, Elzbieta RomanowskaAbstract:The O-specific polysaccharide of the lipopolysaccharide produced by Hafnia alvei strain 1220 contained d-glucose, d-galactose, N-acetyl-d-glucosamine, N-acetyl-l-fucosamine (2-acetamido-2,6-dideoxy-l-galactose), glycerol, and phosphate. It was proved by composition and methylation analyses, Smith degradation, dephosphorylation, and one- and two-dimensional 1H NMR spectroscopy to be a teichoic acid-like polymer with a branched hexasaccharide repeating unit of the following structure:
Andrzej Gamian - One of the best experts on this subject based on the ideXlab platform.
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Structure of the lipopolysaccharide core region of Hafnia alvei strains 1185 and 1204.
Carbohydrate Research, 2000Co-Authors: Joanna Kübler, Ewa Katzenellenbogen, Andrzej Gamian, Maria Bogulska, Andrzej Ejchart, Elzbieta RomanowskaAbstract:Abstract Sugar and methylation analyses using gas chromatography/mass spectrometry and NMR spectroscopy proved that the core oligosaccharides of Hafnia alvei strains 1185 and 1204 have the following formula: Download full-size image where Kdo=3-deoxy-oct-2-ulosonic acid and PPEtN=diphosphorylethanolamine. The structure shown above is a slight modification of the typical core region of H. alvei lipopolysaccharides. The difference refers to one sugar only: terminal galactose is present in the core of strains of 1185 and 1204, while terminal glucose in the typical core.
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Reinvestigation of the O-specific polysaccharides of Hafnia alvei lipopolysaccharides isolated from strains ATCC 13337 and 1187
Carbohydrate Research, 1998Co-Authors: Andrzej Gamian, Ewa Katzenellenbogen, Elzbieta Romanowska, H Grosskurth, Janusz DabrowskiAbstract:Abstract The structure of the O-specific polysaccharides of the lipopolysaccharides produced by Hafnia alvei strains ATCC 13337 and 1187 was reinvestigated. The position of phosphate group in the repeating units of the polysaccharides was established with the aid of 1 H detected, 31 P edited NMR spectra. According to the results obtained, the polysaccharides are teichoic acid-like polymers with the repeating units of the following structure: Download full-size image where Acyl= D -3-hydroxylbutyryl, and 3-O-acetylation was approximately 30%.
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Reinvestigation of the O-specific polysaccharides of Hafnia alvei lipopolysaccharides isolated from strains ATCC 13337 and 1187.
Carbohydrate research, 1998Co-Authors: Andrzej Gamian, Ewa Katzenellenbogen, Elzbieta Romanowska, H Grosskurth, Janusz DabrowskiAbstract:The structure of the O-specific polysaccharides of the lipopolysaccharides produced by Hafnia alvei strains ATCC 13337 and 1187 was reinvestigated. The position of phosphate group in the repeating units of the polysaccharides was established with the aid of 1H detected, 31P edited NMR spectra. According to the results obtained, the polysaccharides are teichoic acid-like polymers with the repeating units of the following structure: [formula: see text] where Acyl = D-3-hydroxylbutyryl, and 3-O-acetylation was approximately 30%.
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The sensitivity of Hafnia alvei strains to the bactericidal effect of serum
FEMS immunology and medical microbiology, 1996Co-Authors: Jankowski S, Stefan Rowiński, Agnieszka Cisowska, Andrzej GamianAbstract:Most Hafnia alvei strains are sensitive to the bactericidal action of normal bovine serum (NBS) as well as to a serum in which the alternative pathway of complement activation has been thermally blocked. Introduction of polysaccharides (PS) to NBS lowers the bactericidal effect. In a serum in which the alternative pathway of complement activation is blocked, PS completely cancels the bacterial effect.
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Structural and serologic characteristics of O-specific polysaccharides of Hafnia alvei PCM 1185 and 1199
Postepy higieny i medycyny doswiadczalnej, 1996Co-Authors: J Kübler, Andrzej Gamian, Katzenellenbogen E, Małgorzata Mieszała, Romanowska EAbstract:This work describes results of studies of O-specific oligosaccharide repeating units from lipopolysaccharides of two related Hafnia alvei serotypes. The linkage between O-antigen and the core region in PCM 1199 LPS has been also established. The O-acetyl residues present in the polysaccharides are involved in formation of epitopes.
Czeslaw Lugowski - One of the best experts on this subject based on the ideXlab platform.
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Novel O-antigen of Hafnia alvei PCM 1195 lipopolysaccharide with a teichoic acid-like structure.
Carbohydrate research, 2009Co-Authors: Tomasz Niedziela, L Kenne, Czeslaw LugowskiAbstract:Abstract The lipopolysaccharide (LPS) of Hafnia alvei strain PCM 1195 was obtained by the hot phenol/water method. The O-specific polysaccharide was released by mild acidic hydrolysis and isolated by gel filtration. The structure of the O-specific polysaccharide was investigated by 1 H, 13 C, and 31 P NMR spectroscopy, MALDI-TOF MS, and GC–MS, accompanied by monosaccharide and methylation analysis. It was concluded that the O-specific polysaccharide is composed of a hexasaccharide repeating units interlinked with a phosphate group: {→4-α- d -Glc p -(1→3)-α- l - Fuc p NAc-(1→3)-[α- d -Glc p -(1→4)]-α- d -Glc p NAc-(1→3)-α- l - Fuc p NAc-(1→4)-α- d -Glc p -(1→P} n .
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Structural studies of the O-specific polysaccharide of Hafnia alvei strain PCM 1207 lipopolysaccharide
European journal of biochemistry, 1999Co-Authors: Wojciech Jachymek, Czeslaw Lugowski, Tomasz Niedziela, Jolanta Czaja, L KenneAbstract:The structure of the O-specific side chains of the Hafnia alvei strain 1209 lipopolysaccharide has been investigated. Methylation analysis and 1H-NMR and 13C-NMR spectroscopy were the principal methods used. It is concluded that the polysaccharide is composed of pentasaccharide repeating units that have the following structure: The relative intensity of the signals from the terminal repeating unit in the 1H-NMR spectrum, the amount of 2,3,6-tri-O -methylgalactose in the methylation analysis, and the matrix-assisted laser-desorption ionisation time-of-flight (MALDI-TOF) mass spectrum of the O-polysaccharide indicated that the structure is also the biological repeating unit and that the O-chains mainly consisted of 8–11 repeating units and, on average, ten repeating units.
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Structural studies of the O-specific chains of Hafnia alvei strains 744, PCM 1194 and PCM 1210 lipopolysaccharides.
European journal of biochemistry, 1997Co-Authors: C Petersson, Czeslaw Lugowski, Tomasz Niedziela, Wojciech Jachymek, A Klonowska, L KenneAbstract:The structures of the O-specific chains of the Hafnia alvei strain 744 and PCM strains 1194 and 1210 lipopolysaccharides have been investigated. Methylation analysis, dephosphorylation, NMR spectroscopy, matrix-assisted laser-desorption ionisation time-of-flight mass spectrometry and fast-atom-bombardment mass spectrometry were the principal methods used. It was concluded that the polysaccharides of strains 744 and PCM 1194 are composed of the same pentasaccharide repeating unit having the following structure: and that the polysaccharide of strain PCM 1210 is composed of a pentasaccharide repeating unit having the following structure:
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Structural studies of the O-specific chain of Hafnia alvei strain PCM 1190 lipopolysaccharide.
Carbohydrate research, 1997Co-Authors: C Petersson, Wojciech Jachymek, Tomasz Niedziela, L Kenne, Czeslaw LugowskiAbstract:The structure of the O-specific side-chain of the lipopolysaccharide of Hafnia alvei strain PCM 1190 has been investigated. Methylation analysis, partial acid hydrolysis, Smith degradation, NMR spectroscopy, MALDI-TOF, and FAB mass spectrometry in combination with collision-induced-decomposition MS/MS were the principal methods used. It was concluded that the polysaccharide is composed of heptasaccharide repeating units having the following structure: [formula: see text] Only 80% of the repeating units are complete, whereas 20% of them are lacking the alpha-D-glucopyranosyl group.
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Structural Studies of the O‐Specific Polysaccharide of Hafnia alvei Strain PCM 1206 Lipopolysaccharide Containing D‐Allothreonine
European journal of biochemistry, 1997Co-Authors: C Petersson, Tomasz Niedziela, Wojciech Jachymek, L Kenne, Przemyslaw Zarzecki, Czeslaw LugowskiAbstract:The structure of the O-specific side-chain of the Hafnia alvei strain PCM 1206 lipopolysaccharide has been investigated. Methylation analysis, partial acid hydrolysis, FAB-MS/MS and 1H-NMR and 13C-NMR spectroscopy were the principal methods used. D-Allothreonine (D-aThr), amide-linked to the D-galacturonic acid, was identified as a constituent in the polysaccharide and the following structure of a pentasaccharide repeating unit was established: [structure: see text].
Janusz Dabrowski - One of the best experts on this subject based on the ideXlab platform.
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Reinvestigation of the O-specific polysaccharides of Hafnia alvei lipopolysaccharides isolated from strains ATCC 13337 and 1187
Carbohydrate Research, 1998Co-Authors: Andrzej Gamian, Ewa Katzenellenbogen, Elzbieta Romanowska, H Grosskurth, Janusz DabrowskiAbstract:Abstract The structure of the O-specific polysaccharides of the lipopolysaccharides produced by Hafnia alvei strains ATCC 13337 and 1187 was reinvestigated. The position of phosphate group in the repeating units of the polysaccharides was established with the aid of 1 H detected, 31 P edited NMR spectra. According to the results obtained, the polysaccharides are teichoic acid-like polymers with the repeating units of the following structure: Download full-size image where Acyl= D -3-hydroxylbutyryl, and 3-O-acetylation was approximately 30%.
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Reinvestigation of the O-specific polysaccharides of Hafnia alvei lipopolysaccharides isolated from strains ATCC 13337 and 1187.
Carbohydrate research, 1998Co-Authors: Andrzej Gamian, Ewa Katzenellenbogen, Elzbieta Romanowska, H Grosskurth, Janusz DabrowskiAbstract:The structure of the O-specific polysaccharides of the lipopolysaccharides produced by Hafnia alvei strains ATCC 13337 and 1187 was reinvestigated. The position of phosphate group in the repeating units of the polysaccharides was established with the aid of 1H detected, 31P edited NMR spectra. According to the results obtained, the polysaccharides are teichoic acid-like polymers with the repeating units of the following structure: [formula: see text] where Acyl = D-3-hydroxylbutyryl, and 3-O-acetylation was approximately 30%.
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Structure of the O-specific polysaccharide, containing a glycerol phosphate substituent, of Hafnia alvei strain 1220 lipopolysaccharide
Carbohydrate research, 1996Co-Authors: Ursula Dabrowski, Ewa Katzenellenbogen, Maria Bogulska, Janusz Dabrowski, Elzbieta RomanowskaAbstract:The O-specific polysaccharide of the lipopolysaccharide produced by Hafnia alvei strain 1220 contained d-glucose, d-galactose, N-acetyl-d-glucosamine, N-acetyl-l-fucosamine (2-acetamido-2,6-dideoxy-l-galactose), glycerol, and phosphate. It was proved by composition and methylation analyses, Smith degradation, dephosphorylation, and one- and two-dimensional 1H NMR spectroscopy to be a teichoic acid-like polymer with a branched hexasaccharide repeating unit of the following structure:
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3 deoxy octulosonic acid containing trisaccharide fragment of an unusual core type of some Hafnia alvei lipopolysaccharides
Biochemical and Biophysical Research Communications, 1993Co-Authors: Ewa Katzenellenbogen, Andrzej Gamian, Elzbieta Romanowska, Ursula Dabrowski, Janusz DabrowskiAbstract:Abstract The 3-deoxy-octulosonic acid-containing trisaccharide was isolated from the carbohydrate material obtained after the acid hydrolysis of lipopolysaccharides of Hafnia alvei strains 2 and 1211 using gel filtration. For purification the trisaccharide fraction was rechromatographed on a Bio-Gel P2 column. On the basis of sugar and methylation analyses and 1 H NMR spectroscopy the trisaccharide was identified as 3-deoxy-7- O --(6- O -acetyl-α-D- galacto pyranosyl)-4- O -(α-L- glycero -D- manno heptopyranosyl)-D- manno -2-octulopyranosic acid. The occurence of such bifurcate Kdo-containing structure has not been previously reported in Enterobacteriaceae.
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Structure of the O−specific polysaccharide containing pentitol phosphate, isolated from Hafnia alvei strain PCM 1191 lipopolysaccharide
European journal of biochemistry, 1993Co-Authors: Andrzej Gamian, Elzbieta Romanowska, Ursula Dabrowski, Janusz DabrowskiAbstract:The O-specific polysaccharide of the lipopolysaccharide produced by Hafnia alvei strain PCM 1191 was shown by composition and methylation analyses, periodate oxidation and one- and two-dimensional 1H- and 31P-NMR spectroscopy to be a polymer of a branched hexasaccharide repeating units having the structure: [formula: see text] where LAraol = L-arabitol The polysaccharide of 1191 strain has teichoic-acid-like character. Its peculiar feature is the presence of arabitol phosphate, a component observed for the first time in bacterial lipopolysaccharides.
Tomasz Niedziela - One of the best experts on this subject based on the ideXlab platform.
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Novel O-antigen of Hafnia alvei PCM 1195 lipopolysaccharide with a teichoic acid-like structure.
Carbohydrate research, 2009Co-Authors: Tomasz Niedziela, L Kenne, Czeslaw LugowskiAbstract:Abstract The lipopolysaccharide (LPS) of Hafnia alvei strain PCM 1195 was obtained by the hot phenol/water method. The O-specific polysaccharide was released by mild acidic hydrolysis and isolated by gel filtration. The structure of the O-specific polysaccharide was investigated by 1 H, 13 C, and 31 P NMR spectroscopy, MALDI-TOF MS, and GC–MS, accompanied by monosaccharide and methylation analysis. It was concluded that the O-specific polysaccharide is composed of a hexasaccharide repeating units interlinked with a phosphate group: {→4-α- d -Glc p -(1→3)-α- l - Fuc p NAc-(1→3)-[α- d -Glc p -(1→4)]-α- d -Glc p NAc-(1→3)-α- l - Fuc p NAc-(1→4)-α- d -Glc p -(1→P} n .
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Structural studies of the O-specific polysaccharide of Hafnia alvei strain PCM 1207 lipopolysaccharide
European journal of biochemistry, 1999Co-Authors: Wojciech Jachymek, Czeslaw Lugowski, Tomasz Niedziela, Jolanta Czaja, L KenneAbstract:The structure of the O-specific side chains of the Hafnia alvei strain 1209 lipopolysaccharide has been investigated. Methylation analysis and 1H-NMR and 13C-NMR spectroscopy were the principal methods used. It is concluded that the polysaccharide is composed of pentasaccharide repeating units that have the following structure: The relative intensity of the signals from the terminal repeating unit in the 1H-NMR spectrum, the amount of 2,3,6-tri-O -methylgalactose in the methylation analysis, and the matrix-assisted laser-desorption ionisation time-of-flight (MALDI-TOF) mass spectrum of the O-polysaccharide indicated that the structure is also the biological repeating unit and that the O-chains mainly consisted of 8–11 repeating units and, on average, ten repeating units.
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Non-typical lipopolysaccharide core regions of some Hafnia alvei strains: structural and serological studies.
FEMS immunology and medical microbiology, 1999Co-Authors: Elzbieta Romanowska, Ewa Katzenellenbogen, Tomasz Niedziela, Wojciech Jachymek, Maria Bogulska, Czesław ŁugowskiAbstract:The lipopolysaccharides of Hafnia alvei strains 23, 1222 and 39 were found to have non-typical core region. On the basis of sugar and methylation analyses, 1H-nuclear magnetic resonance spectra and matrix-assisted laser-desorption ionization-time of flight mass spectrometry, it was concluded that the core oligosaccharide of strains 23 and 1222 has the same structure as Escherichia coli R4 core region, and the core oligosaccharide of strain 39 has the structure of Salmonella Ra core. Using the serological methods (passive hemagglutination, enzyme-linked immunosorbent assay and immunoblotting) and the anti-conjugate sera directed against E. coli R4 and Salmonella Ra core oligosaccharides we have confirmed the structural results presented above.
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Structural studies of the O-specific chains of Hafnia alvei strains 744, PCM 1194 and PCM 1210 lipopolysaccharides.
European journal of biochemistry, 1997Co-Authors: C Petersson, Czeslaw Lugowski, Tomasz Niedziela, Wojciech Jachymek, A Klonowska, L KenneAbstract:The structures of the O-specific chains of the Hafnia alvei strain 744 and PCM strains 1194 and 1210 lipopolysaccharides have been investigated. Methylation analysis, dephosphorylation, NMR spectroscopy, matrix-assisted laser-desorption ionisation time-of-flight mass spectrometry and fast-atom-bombardment mass spectrometry were the principal methods used. It was concluded that the polysaccharides of strains 744 and PCM 1194 are composed of the same pentasaccharide repeating unit having the following structure: and that the polysaccharide of strain PCM 1210 is composed of a pentasaccharide repeating unit having the following structure:
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Structural studies of the O-specific chain of Hafnia alvei strain PCM 1190 lipopolysaccharide.
Carbohydrate research, 1997Co-Authors: C Petersson, Wojciech Jachymek, Tomasz Niedziela, L Kenne, Czeslaw LugowskiAbstract:The structure of the O-specific side-chain of the lipopolysaccharide of Hafnia alvei strain PCM 1190 has been investigated. Methylation analysis, partial acid hydrolysis, Smith degradation, NMR spectroscopy, MALDI-TOF, and FAB mass spectrometry in combination with collision-induced-decomposition MS/MS were the principal methods used. It was concluded that the polysaccharide is composed of heptasaccharide repeating units having the following structure: [formula: see text] Only 80% of the repeating units are complete, whereas 20% of them are lacking the alpha-D-glucopyranosyl group.
