The Experts below are selected from a list of 4467 Experts worldwide ranked by ideXlab platform
David F Archer - One of the best experts on this subject based on the ideXlab platform.
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levonorgestrel effects on serum androgens sex hormone binding globulin levels Hair Shaft diameter and sexual function
Fertility and Sterility, 2010Co-Authors: G Kovalevsky, Susan A Ballagh, Frank Z Stanczyk, Joann Cooper, David F ArcherAbstract:Objective To determine the effects of levonorgestrel (LNG) on serum androgens, sex hormone–binding globulin (SHBG), Hair Shaft diameter, and sexual function in women. Design Substudy of a prospective randomized double-blind study in women using an LNG SC implant (LNG-SI), who were treated with doxycycline or placebo. Setting Medical school department of obstetrics and gynecology. Patient(s) Forty women were enrolled; 36 completed the study. Intervention(s) Participants were randomized to doxycycline 20 mg or an identical placebo orally twice a day after LNG-SI insertion. Main Outcome Measure(s) Serum levels of total T (TT), free T (FT), androstenedione (A), dehydroepiandrosterone sulfate (DHEAS), dihydrotestosterone (DHT), androstanediol glucuronide (AG), SHBG, and LNG; Hair Shaft diameter; and sexual function using the Brief Index of Sexual Function for Women and the Arizona Sexual Experiences Scale were assessed. Result(s) Serum TT, A, DHT, DHEAS, and SHBG declined after LNG-SI insertion. No changes were found in FT, AG, Hair Shaft diameter, or sexual function. Serum LNG correlated with SHBG levels. There were no differences between the placebo and doxycycline groups. Conclusion(s) LNG reduced serum TT, A, DHT, DHEAS, and SHBG but had no effect on sexual function or markers of androgen bioactivity.
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Levonorgestrel effects on serum androgens, sex hormone–binding globulin levels, Hair Shaft diameter, and sexual function
Fertility and Sterility, 2009Co-Authors: G Kovalevsky, Susan A Ballagh, Frank Z Stanczyk, Joann Cooper, Jennifer Lee, David F ArcherAbstract:Objective To determine the effects of levonorgestrel (LNG) on serum androgens, sex hormone–binding globulin (SHBG), Hair Shaft diameter, and sexual function in women. Design Substudy of a prospective randomized double-blind study in women using an LNG SC implant (LNG-SI), who were treated with doxycycline or placebo. Setting Medical school department of obstetrics and gynecology. Patient(s) Forty women were enrolled; 36 completed the study. Intervention(s) Participants were randomized to doxycycline 20 mg or an identical placebo orally twice a day after LNG-SI insertion. Main Outcome Measure(s) Serum levels of total T (TT), free T (FT), androstenedione (A), dehydroepiandrosterone sulfate (DHEAS), dihydrotestosterone (DHT), androstanediol glucuronide (AG), SHBG, and LNG; Hair Shaft diameter; and sexual function using the Brief Index of Sexual Function for Women and the Arizona Sexual Experiences Scale were assessed. Result(s) Serum TT, A, DHT, DHEAS, and SHBG declined after LNG-SI insertion. No changes were found in FT, AG, Hair Shaft diameter, or sexual function. Serum LNG correlated with SHBG levels. There were no differences between the placebo and doxycycline groups. Conclusion(s) LNG reduced serum TT, A, DHT, DHEAS, and SHBG but had no effect on sexual function or markers of androgen bioactivity.
G Kovalevsky - One of the best experts on this subject based on the ideXlab platform.
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levonorgestrel effects on serum androgens sex hormone binding globulin levels Hair Shaft diameter and sexual function
Fertility and Sterility, 2010Co-Authors: G Kovalevsky, Susan A Ballagh, Frank Z Stanczyk, Joann Cooper, David F ArcherAbstract:Objective To determine the effects of levonorgestrel (LNG) on serum androgens, sex hormone–binding globulin (SHBG), Hair Shaft diameter, and sexual function in women. Design Substudy of a prospective randomized double-blind study in women using an LNG SC implant (LNG-SI), who were treated with doxycycline or placebo. Setting Medical school department of obstetrics and gynecology. Patient(s) Forty women were enrolled; 36 completed the study. Intervention(s) Participants were randomized to doxycycline 20 mg or an identical placebo orally twice a day after LNG-SI insertion. Main Outcome Measure(s) Serum levels of total T (TT), free T (FT), androstenedione (A), dehydroepiandrosterone sulfate (DHEAS), dihydrotestosterone (DHT), androstanediol glucuronide (AG), SHBG, and LNG; Hair Shaft diameter; and sexual function using the Brief Index of Sexual Function for Women and the Arizona Sexual Experiences Scale were assessed. Result(s) Serum TT, A, DHT, DHEAS, and SHBG declined after LNG-SI insertion. No changes were found in FT, AG, Hair Shaft diameter, or sexual function. Serum LNG correlated with SHBG levels. There were no differences between the placebo and doxycycline groups. Conclusion(s) LNG reduced serum TT, A, DHT, DHEAS, and SHBG but had no effect on sexual function or markers of androgen bioactivity.
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Levonorgestrel effects on serum androgens, sex hormone–binding globulin levels, Hair Shaft diameter, and sexual function
Fertility and Sterility, 2009Co-Authors: G Kovalevsky, Susan A Ballagh, Frank Z Stanczyk, Joann Cooper, Jennifer Lee, David F ArcherAbstract:Objective To determine the effects of levonorgestrel (LNG) on serum androgens, sex hormone–binding globulin (SHBG), Hair Shaft diameter, and sexual function in women. Design Substudy of a prospective randomized double-blind study in women using an LNG SC implant (LNG-SI), who were treated with doxycycline or placebo. Setting Medical school department of obstetrics and gynecology. Patient(s) Forty women were enrolled; 36 completed the study. Intervention(s) Participants were randomized to doxycycline 20 mg or an identical placebo orally twice a day after LNG-SI insertion. Main Outcome Measure(s) Serum levels of total T (TT), free T (FT), androstenedione (A), dehydroepiandrosterone sulfate (DHEAS), dihydrotestosterone (DHT), androstanediol glucuronide (AG), SHBG, and LNG; Hair Shaft diameter; and sexual function using the Brief Index of Sexual Function for Women and the Arizona Sexual Experiences Scale were assessed. Result(s) Serum TT, A, DHT, DHEAS, and SHBG declined after LNG-SI insertion. No changes were found in FT, AG, Hair Shaft diameter, or sexual function. Serum LNG correlated with SHBG levels. There were no differences between the placebo and doxycycline groups. Conclusion(s) LNG reduced serum TT, A, DHT, DHEAS, and SHBG but had no effect on sexual function or markers of androgen bioactivity.
Robert M Hoffman - One of the best experts on this subject based on the ideXlab platform.
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protocols for gelfoam histoculture of Hair Shaft producing mouse whisker follicles containing nestin gfp expressing Hair follicle associated pluripotent hap stem cells for long time periods
Methods of Molecular Biology, 2016Co-Authors: Yasuyuki Amoh, Fang Liu, Wenluo Cao, Robert M HoffmanAbstract:Gelfoam(®)-histocultured whisker follicles from nestin-driven-green fluorescent protein (ND-GFP) mice produced growing pigmented and unpigmented Hair Shafts. Hair-Shaft length increased rapidly by day 4 and continued growing until at least day 12 after which the Hair-Shaft length was constant. By day 63 in histoculture, the number of ND-GFP Hair follicle-associated pluripotent (HAP) stem cells increased significantly and the follicles were intact. Three-dimensional Gelfoam(®) histoculture of Hair follicles can provide a very long-term period for evaluating novel agents to promote Hair growth.
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extensive Hair Shaft growth after mouse whisker follicle isolation cryopreservation and transplantation in nude mice
PLOS ONE, 2015Co-Authors: Fang Liu, Yasuyuki Amoh, Wenluo Cao, Benjamin Tran, Satoshi Kajiura, Robert M HoffmanAbstract:We previously demonstrated that whole Hair follicles could be cryopreserved to maintain their stem-cells differentation potential. In the present study, we demonstrated that cryopreserved mouse whisker Hair follicles maintain their Hair growth potential. DMSO better cryopreserved mouse whisker follicles compared to glycerol. Cryopreserved Hair follicles also maintained the Hair follicle-associated-pluripotent (HAP) stem cells, evidenced by P75NTR expression. Subcutaneous transplantation of DMSO-cryopreserved Hair follicles in nude mice resulted in extensive Hair fiber growth over 8 weeks, indicating the functional recovery of Hair Shaft growth of cryopreserved Hair follicles.
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extensive Hair Shaft elongation by isolated mouse whisker follicles in very long term gelfoam histoculture
PLOS ONE, 2015Co-Authors: Wenluo Cao, Fang Liu, Sumiyuki Mii, Yasuyuki Amoh, Robert M HoffmanAbstract:We have previously studied mouse whisker follicles in Gelfoam® histoculture to determine the role of nestin-expressing plutipotent stem cells, located within the follicle, in the growth of the follicular sensory nerve. Long-term Gelfoam® whisker histoculture enabled Hair follicle nestin-expressing stem cells to promote the extensive elongation of the whisker sensory nerve, which contained axon fibers. Transgenic mice in which the nestin promoter drives green fluorescent protein (ND-GFP) were used as the source of the whiskers allowing imaging of the nestin-expressing stem cells as they formed the follicular sensory nerve. In the present report, we show that Gelfoam®-histocultured whisker follicles produced growing pigmented and unpigmented Hair Shafts. Hair-Shaft length increased rapidly by day-4 and continued growing until at least day-12 after which the Hair-Shaft length was constant. By day-63 in histoculture, the number of ND-GFP Hair follicle stem cells increased significantly and the follicles were intact. The present study shows that Gelfoam® histoculture can support extensive Hair-Shaft growth as well as Hair follicle sensory-nerve growth from isolated Hair follicles which were maintained over very long periods of time. Gelfoam® histoculture of Hair follicles can provide a very long-term period for evaluating novel agents to promote Hair growth.
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Hair Shaft elongation of mouse whiskers in Gelfoam® histoculture.
2015Co-Authors: Wenluo Cao, Fang Liu, Sumiyuki Mii, Yasuyuki Amoh, Robert M HoffmanAbstract:Time-course images of Hair Shaft growth from individual mouse whisker follicles, isolated from nestin-driven green fluorescent protein (ND-GFP) mice, histocultured on Gelfoam®. Green fluorescence was from the ND-GFP-expressing stem cells in the whisker Hair follicles which were enriched during 63 days of histoculture in vitro. Hair Shafts lengthened rapidly in the first 4 days, extended over 9–12 days, and remained the same length until day 63.
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Hair Shaft elongation follicle growth and spontaneous regression in long term gelatin sponge supported histoculture of human scalp skin
Proceedings of the National Academy of Sciences of the United States of America, 1992Co-Authors: Lingna Li, Leonid Margolis, Ralf Paus, Robert M HoffmanAbstract:Abstract In order to better understand the molecular mechanisms of human Hair growth control and to test Hair growth-modulatory drugs, appropriate in vitro models are required. Here, we report the long-term growth, Shaft elongation, and spontaneous regression of human Hair follicles in histoculture of intact scalp skin. Human scalp skin with abundant Hair follicles in various stages of the Hair growth cycle was grown for up to 40 days in a gelatin sponge-supported histoculture system at the air/liquid interface. Isolated follicles placed in the gelatin-sponge matrix also supported Hair Shaft elongation, with the Hair follicle cells remaining proliferative and viable for very long periods. Hair Shaft elongation occurred mainly during the first 10 days of histoculture of both intact skin and isolated follicles. However, Hair follicles were viable and follicle keratinocytes continued to incorporate [3H]thymidine for up to several weeks after Shaft elongation had ceased as shown by fluorescent-dye double staining, measured by confocal laser scanning microscopy, and by histological autoradiography of [3H]thymidine incorporation, respectively. Hair follicles could continue their cycle in histoculture; for example, apparent spontaneous catagen induction was observed both histologically and by the actual regression of the Hair follicle. In addition, vellus follicles were shown to be viable at day 40 after initiation of culture. In the histocultured human scalp we demonstrated the association of mast cells with anagen follicles and macrophages with catagen follicles, suggesting a role of these cells in the Hair cycle. This histoculture technique should serve as a powerful tool for future Hair research in the human system as well as a screening assay for compounds that can perturb the Hair cycle.
Joann Cooper - One of the best experts on this subject based on the ideXlab platform.
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levonorgestrel effects on serum androgens sex hormone binding globulin levels Hair Shaft diameter and sexual function
Fertility and Sterility, 2010Co-Authors: G Kovalevsky, Susan A Ballagh, Frank Z Stanczyk, Joann Cooper, David F ArcherAbstract:Objective To determine the effects of levonorgestrel (LNG) on serum androgens, sex hormone–binding globulin (SHBG), Hair Shaft diameter, and sexual function in women. Design Substudy of a prospective randomized double-blind study in women using an LNG SC implant (LNG-SI), who were treated with doxycycline or placebo. Setting Medical school department of obstetrics and gynecology. Patient(s) Forty women were enrolled; 36 completed the study. Intervention(s) Participants were randomized to doxycycline 20 mg or an identical placebo orally twice a day after LNG-SI insertion. Main Outcome Measure(s) Serum levels of total T (TT), free T (FT), androstenedione (A), dehydroepiandrosterone sulfate (DHEAS), dihydrotestosterone (DHT), androstanediol glucuronide (AG), SHBG, and LNG; Hair Shaft diameter; and sexual function using the Brief Index of Sexual Function for Women and the Arizona Sexual Experiences Scale were assessed. Result(s) Serum TT, A, DHT, DHEAS, and SHBG declined after LNG-SI insertion. No changes were found in FT, AG, Hair Shaft diameter, or sexual function. Serum LNG correlated with SHBG levels. There were no differences between the placebo and doxycycline groups. Conclusion(s) LNG reduced serum TT, A, DHT, DHEAS, and SHBG but had no effect on sexual function or markers of androgen bioactivity.
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Levonorgestrel effects on serum androgens, sex hormone–binding globulin levels, Hair Shaft diameter, and sexual function
Fertility and Sterility, 2009Co-Authors: G Kovalevsky, Susan A Ballagh, Frank Z Stanczyk, Joann Cooper, Jennifer Lee, David F ArcherAbstract:Objective To determine the effects of levonorgestrel (LNG) on serum androgens, sex hormone–binding globulin (SHBG), Hair Shaft diameter, and sexual function in women. Design Substudy of a prospective randomized double-blind study in women using an LNG SC implant (LNG-SI), who were treated with doxycycline or placebo. Setting Medical school department of obstetrics and gynecology. Patient(s) Forty women were enrolled; 36 completed the study. Intervention(s) Participants were randomized to doxycycline 20 mg or an identical placebo orally twice a day after LNG-SI insertion. Main Outcome Measure(s) Serum levels of total T (TT), free T (FT), androstenedione (A), dehydroepiandrosterone sulfate (DHEAS), dihydrotestosterone (DHT), androstanediol glucuronide (AG), SHBG, and LNG; Hair Shaft diameter; and sexual function using the Brief Index of Sexual Function for Women and the Arizona Sexual Experiences Scale were assessed. Result(s) Serum TT, A, DHT, DHEAS, and SHBG declined after LNG-SI insertion. No changes were found in FT, AG, Hair Shaft diameter, or sexual function. Serum LNG correlated with SHBG levels. There were no differences between the placebo and doxycycline groups. Conclusion(s) LNG reduced serum TT, A, DHT, DHEAS, and SHBG but had no effect on sexual function or markers of androgen bioactivity.
Susan A Ballagh - One of the best experts on this subject based on the ideXlab platform.
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levonorgestrel effects on serum androgens sex hormone binding globulin levels Hair Shaft diameter and sexual function
Fertility and Sterility, 2010Co-Authors: G Kovalevsky, Susan A Ballagh, Frank Z Stanczyk, Joann Cooper, David F ArcherAbstract:Objective To determine the effects of levonorgestrel (LNG) on serum androgens, sex hormone–binding globulin (SHBG), Hair Shaft diameter, and sexual function in women. Design Substudy of a prospective randomized double-blind study in women using an LNG SC implant (LNG-SI), who were treated with doxycycline or placebo. Setting Medical school department of obstetrics and gynecology. Patient(s) Forty women were enrolled; 36 completed the study. Intervention(s) Participants were randomized to doxycycline 20 mg or an identical placebo orally twice a day after LNG-SI insertion. Main Outcome Measure(s) Serum levels of total T (TT), free T (FT), androstenedione (A), dehydroepiandrosterone sulfate (DHEAS), dihydrotestosterone (DHT), androstanediol glucuronide (AG), SHBG, and LNG; Hair Shaft diameter; and sexual function using the Brief Index of Sexual Function for Women and the Arizona Sexual Experiences Scale were assessed. Result(s) Serum TT, A, DHT, DHEAS, and SHBG declined after LNG-SI insertion. No changes were found in FT, AG, Hair Shaft diameter, or sexual function. Serum LNG correlated with SHBG levels. There were no differences between the placebo and doxycycline groups. Conclusion(s) LNG reduced serum TT, A, DHT, DHEAS, and SHBG but had no effect on sexual function or markers of androgen bioactivity.
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Levonorgestrel effects on serum androgens, sex hormone–binding globulin levels, Hair Shaft diameter, and sexual function
Fertility and Sterility, 2009Co-Authors: G Kovalevsky, Susan A Ballagh, Frank Z Stanczyk, Joann Cooper, Jennifer Lee, David F ArcherAbstract:Objective To determine the effects of levonorgestrel (LNG) on serum androgens, sex hormone–binding globulin (SHBG), Hair Shaft diameter, and sexual function in women. Design Substudy of a prospective randomized double-blind study in women using an LNG SC implant (LNG-SI), who were treated with doxycycline or placebo. Setting Medical school department of obstetrics and gynecology. Patient(s) Forty women were enrolled; 36 completed the study. Intervention(s) Participants were randomized to doxycycline 20 mg or an identical placebo orally twice a day after LNG-SI insertion. Main Outcome Measure(s) Serum levels of total T (TT), free T (FT), androstenedione (A), dehydroepiandrosterone sulfate (DHEAS), dihydrotestosterone (DHT), androstanediol glucuronide (AG), SHBG, and LNG; Hair Shaft diameter; and sexual function using the Brief Index of Sexual Function for Women and the Arizona Sexual Experiences Scale were assessed. Result(s) Serum TT, A, DHT, DHEAS, and SHBG declined after LNG-SI insertion. No changes were found in FT, AG, Hair Shaft diameter, or sexual function. Serum LNG correlated with SHBG levels. There were no differences between the placebo and doxycycline groups. Conclusion(s) LNG reduced serum TT, A, DHT, DHEAS, and SHBG but had no effect on sexual function or markers of androgen bioactivity.
