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Maria Eugenia D’amato - One of the best experts on this subject based on the ideXlab platform.
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Isolation and characterization of microsatellite markers in the South African abalone (Haliotis midae)
Molecular Ecology Notes, 2004Co-Authors: Aletta E. Bester, Ruhan Slabbert, Maria Eugenia D’amatoAbstract:We report the isolation and characterization of 11 polymorphic microsatellite loci in the South African abalone Haliotis midae. These loci showed a range of five to 21 alleles per locus and observed heterozygosities ranging from 0.14 to 0.93 in a wild population of 32 individuals. All loci except four conformed to Hardy–Weinberg expectations and did not show linkage disequilibrium. The polymorphism exhibited at these loci indicate that they would be useful in determining levels of genetic variability in natural and commercial Haliotis midae populations as well as in parentage and Quantitative Trait Loci (QTL) analysis in hatchery reared abalone.
Carolyn S Friedman - One of the best experts on this subject based on the ideXlab platform.
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precipitous declines in pinto abalone Haliotis kamtschatkana kamtschatkana abundance in the san juan archipelago washington usa despite statewide fishery closure
Canadian Journal of Fisheries and Aquatic Sciences, 2008Co-Authors: Don Rothausd P P Rothaus, Brent Vadopalas, Carolyn S FriedmanAbstract:Pinto abalone (Haliotis kamtschatkana kamtschatkana) index stations in the San Juan Archipelago were systematically monitored by the Washington Department of Fish and Wildlife from 1992 through 200...
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withering syndrome in farmed red abalone Haliotis rufescens thermal induction and association with a gastrointestinal rickettsiales like prokaryote
Journal of Aquatic Animal Health, 2000Co-Authors: James D Moore, Thea T Robbins, Carolyn S FriedmanAbstract:Abstract Withering syndrome (WS) is a chronic wasting disease responsible for mass mortality in wild populations of black abalone Haliotis cracherodii. The etiology of WS is uncertain with limited evidence for the role of a gastrointestinal Rickettsiales-like prokaryote (RLP). We documented for the first time the occurrence of animals with clinical signs of WS and associated morphological changes in another haliotid species, the red abalone H. rufescens. In this study, 60 juvenile red abalone (8 cm) were randomly selected from a farmed population raised at 14°C that was known to have low-intensity RLP infections but lacked clinical signs of WS. The abalone were held in triplicate containers receiving water of approximately 14.7°C (Control, Co) or 18.5°C (elevated temperature, ET) and were fed equally for 220 d. Survival was 100% (30/30) for the Co group and 67% (20/30) for the ET group. The ET group animals had higher RLP infection intensities and showed more clinical signs (mantle retraction, lower weigh...
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Pseudoklossia Haliotis sp. n. (Apicomplexa) from the Kidney of California Abalone, Haliotis spp. (Mollusca)
Journal of Invertebrate Pathology, 1995Co-Authors: Carolyn S Friedman, George R. Gardner, Ronald P. Hedrick, Mark Stephenson, Richard J. Cawthorn, Steve J. UptonAbstract:A coccidian of the genus Pseudoklossia Leger and Duboscq, 1915 (Apicomplexa) occurs in the kidneys of abalone, Haliotis spp. (Mollusca), throughout waters off California. Parasites are often numerous in renal tissues, and developmental stages include oocysts, macrogametes, microgametocytes, and meronts. Renal coccidian infections occur in uninfected abalone after exposure to water occupied previously by infected animals. The life cycle of this parasite appears to be monoxenous and the coccidian evokes no host inflammatory response. We term this coccidian Pseudoklossia Haliotis sp. n. and describe the morphologic characteristics of the endogenous stages and oocysts. This represents only the seventh named species described from the genus.
Chongming Wang - One of the best experts on this subject based on the ideXlab platform.
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RNA-seq of HaHV-1-infected abalones reveals a common transcriptional signature of Malacoherpesviruses
Scientific Reports, 2019Co-Authors: Umberto Rosani, Ya Nan Li, Chongming WangAbstract:Haliotid herpesvirus-1 (HaHV-1) is the viral agent causative of abalone viral ganglioneuritis, a disease that has severely affected gastropod aquaculture. Although limited, the sequence similarity between HaHV-1 and Ostreid herpesvirus-1 supported the assignment of both viruses to Malacoherpesviridae, a Herpesvirales family distantly related with other viruses. In this study, we reported the first transcriptional data of HaHV-1, obtained from an experimental infection of Haliotis diversicolor supertexta. We also sequenced the genome draft of the Chinese HaHV-1 variant isolated in 2003 (HaHV-1-CN2003) by PacBio technology. Analysis of 13 million reads obtained from 3 RNA samples at 60 hours post injection (hpi) allowed the prediction of 51 new ORFs for a total of 117 viral genes and the identification of 207 variations from the reference genome, consisting in 135 Single Nucleotide Polymorphisms (SNPs) and 72 Insertions or Deletions (InDels). The pairing of genomic and transcriptomic data supported the identification of 60 additional SNPs, representing viral transcriptional variability and preferentially grouped in hotspots. The expression analysis of HaHV-1 ORFs revealed one putative secreted protein, two putative capsid proteins and a possible viral capsid protease as the most expressed genes and demonstrated highly synchronized viral expression patterns of the 3 infected animals at 60 hpi. Quantitative reverse transcription data of 37 viral genes supported the burst of viral transcription at 30 and 60 hpi during the 72 hours of the infection experiment, and allowed the distinction between early and late viral genes.
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Dual Transcriptomic Analysis Reveals a Delayed Antiviral Response of Haliotis diversicolor supertexta against Haliotid Herpesvirus-1
MDPI AG, 2019Co-Authors: Changming Bai, Umberto Rosani, Lu-sheng Xin, Shu-min Zhang, Chongming WangAbstract:Haliotid herpesvirus-1 (HaHV-1) is the first identified gastropod herpesvirus, causing a highly lethal neurologic disease of abalone species. The genome of HaHV-1 has been sequenced, but the functions of the putative genes and their roles during infection are still poorly understood. In the present study, transcriptomic profiles of Haliotis diversicolor supertexta at 0, 24 and 60 h post injection (hpi) with HaHV-1 were characterized through high-throughput RNA sequencing. A total of 448 M raw reads were obtained and assembled into 2.08 × 105 unigenes with a mean length of 1486 bp and an N50 of 2455 bp. Although we detected increased HaHV-1 DNA loads and active viral expression at 24 hpi, this evidence was not linked to significant changes of host transcriptomic profiles between 0 and 24 hpi, whereas a rich immune-related gene set was over-expressed at 60 hpi. These results indicate that, at least at the beginning of HaHV-1 infection, the virus can replicate with no activation of the host immune response. We propose that HaHV-1 may evolve more effective strategies to modulate the host immune response and hide during replication, so that it could evade the immune surveillance at the early stage of infection
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Susceptibility of two abalone species, Haliotis diversicolor supertexta and Haliotis discus hannai, to Haliotid herpesvirus 1 infection
Journal of invertebrate pathology, 2018Co-Authors: Changming Bai, P.-s. Chang, Jing-zhe Jiang, Lu-sheng Xin, Jiang-yong Wang, Chongming WangAbstract:Abalone viral ganglioneuritis (AVG), caused by Haliotid herpesvirus-1 (HaHV-1) infection, has been reported as the main cause of mortality and heavy losses of wild and cultivated abalone in Taiwan and Australia since 2003. HaHV-1 DNA has also been reported in diseased abalone collected in early 2000s in China. However, no data is available about the susceptibility, disease process and pathological changes of HaHV-1 infection in the primary cultivated abalone species in China. In the present study, two cultivated abalone species, Haliotis diversicolor supertexta and Haliotis discus hannai, were challenged with HaHV-1-CN2003 collected in 2003 in China using three different methods. Results showed that H. diversicolor supertexta was highly susceptible to HaHV-1-CN2003 infection and suffered acute mortality using all three challenge methods. H. discus hannai was not susceptible to the viral infection. Histopathology combined with transmission electron microscopy and quantitative PCR analysis revealed that the tropism of HaHV-1-CN2003 includes both neural tissue and haemocytes.
Jurgen Markl - One of the best experts on this subject based on the ideXlab platform.
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gene structure and hemocyanin isoform hth2 from the mollusc Haliotis tuberculata indicate early and late intron hot spots
Gene, 2002Co-Authors: Benjamin Altenhein, Jurgen Markl, Bernhard LiebAbstract:Abstract We have cloned and sequenced cDNAs coding for the complete primary structure of HtH2, the second hemocyanin isoform of the marine gastropod Haliotis tuberculata. The deduced protein sequence comprises 3399 amino acids, corresponding to a molecular mass of 392 kDa. It shares only 66% of structural identity with the previously analysed first isoform HtH1, and according to a molecular clock, the two isoforms of Haliotis hemocyanin separated ca. 320 million years ago. By genomic polymerase chain reaction and 5′ race, we have also sequenced the complete gene of HtH2 (18,598 bp), except of the 5′ region in front of the secreted protein. It encompasses 15 exons and 14 introns and shows several microsatellite-rich regions. It mirrors the modular structure of the encoded hemocyanin subunit, with a linear arrangement of eight different functional units separated and bordered by seven phase 1 ‘linker introns’. In addition, within regions encoding three of the functional units, the HtH2 gene contains six ‘internal introns’. Comparison to previously sequenced genes of Octopus dofleini hemocyanin and Haliotis hemocyanin isoform (HtH1) suggests Precambrian and Palaeocoic hot spot of intron gains, followed by 320 million years of absolute stasis.
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abalone Haliotis tuberculata hemocyanin type 1 hth1
FEBS Journal, 1999Co-Authors: Henning Keller, Benjamin Altenhein, Dagmar Gebauer, Stefanie Richter, Bernhard Lieb, Sigmar Stricker, Jurgen MarklAbstract:We have identified two separate hemocyanin types (HtH1 and HtH2) in the European abalone Haliotis tuberculata. HtH1/HtH2 hybrid molecules were not found. By selective dissociation of HtH2 we isolated HtH1 which, as revealed by electron microscopy and SDS/PAGE, is present as didecamers of a ≈ 400 kDa subunit. Immunologically, HtH1 and HtH2 correspond to keyhole limpet hemocyanin (KLH)1 and KLH2, respectively, the two well-studied hemocyanin types of the closely related marine gastropod Megathura crenulata. On the basis of limited proteolytic cleavage, two-dimensional immunoelectrophoresis, SDS/PAGE and N-terminal sequencing, we identified eight different 40–60 kDa functional units in HtH1, termed HtH1-a to HtH1-h, and determined their linear arrangement within the elongated subunit. From Haliotis mantle tissue, rich in hemocyanin-producing pore cells, we isolated mRNA and constructed a cDNA library. By expression screening with HtH-specific rabbit antibodies, a cDNA clone was isolated and sequenced which codes for the three C-terminal functional units f, g and h of HtH1. Their sequences were aligned to those available from other molluscs, notably to functional unit f and functional unit g from the cephalopod Octopus dofleini. HtH1-f, which is the first sequenced functional unit of type f from a gastropod hemocyanin, corresponds to functional unit f from Octopus. Also functional unit g from Haliotis and Octopus correspond to each other. HtH1-h is a gastropod hemocyanin functional unit type which is absent in cephalopods and has not been sequenced previously. It exhibits a unique tail extension of ≈ 95 amino acids, which is lacking in functional units a to g and aligns with a published peptide sequence of 48 amino acids from functional unit h of Helix pomatia hemocyanin. The new Haliotis sequences are discussed with respect to their counterparts in Octopus, the 15 A three-dimensional reconstruction of the KLH1 didecamer from electron micrographs, and the recent 2.3 A X-ray structure of functional unit g from Octopus hemocyanin.
Aletta E. Bester - One of the best experts on this subject based on the ideXlab platform.
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Isolation and characterization of microsatellite markers in the South African abalone (Haliotis midae)
Molecular Ecology Notes, 2004Co-Authors: Aletta E. Bester, Ruhan Slabbert, Maria Eugenia D’amatoAbstract:We report the isolation and characterization of 11 polymorphic microsatellite loci in the South African abalone Haliotis midae. These loci showed a range of five to 21 alleles per locus and observed heterozygosities ranging from 0.14 to 0.93 in a wild population of 32 individuals. All loci except four conformed to Hardy–Weinberg expectations and did not show linkage disequilibrium. The polymorphism exhibited at these loci indicate that they would be useful in determining levels of genetic variability in natural and commercial Haliotis midae populations as well as in parentage and Quantitative Trait Loci (QTL) analysis in hatchery reared abalone.
